From carl.hobbs <@t> kcl.ac.uk Tue Mar 1 02:52:04 2005 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] re GFP Message-ID: <002301c51e3b$f0dc7290$e8345c9f@Carlos> I'll concur with john coppens . I have done std pwax processing and still maintained good signal, provided I aq mounted in anti fade mountant. I cut resin sections at 4microns, again mounting with antifade mountant and was able to maintain strong enough signal to do deconvolution/ z stacks. . Again, I concur with John's suggestion to use an anti GFP Ab reagent. I use Molecular Probes'. Some pics of FFPWs of fly embryos immunostained for antiGFP are in the gallery on this site http://www.immunoportal.com/ From mucram11 <@t> comcast.net Tue Mar 1 06:08:55 2005 From: mucram11 <@t> comcast.net (pam marcum) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] ASCP - lots of questions!!! References: Message-ID: <42245B57.000001.02828@YOUR-4DI1S53IME> Amen Pearl. We histologist are often still thought of as the ugly step children in the laboratory and the only way we can become swans is to realize we can do it and get the education the other areas of ASCP and CLIA require. It is a big bullet to bite however those who are grandfathered in aren't required to go back for a degree. We just need keep up now. NSH has helped us and has given us a way to get CEUs however, they have been slow in getting to the education issues with ASCP as we histologist fought it. Those of us old enough to remember should start reminding every one about the early nineties when the CD markers were first coming into IHC and some hospitals starting to develop these labs. A large number of them thought they needed hematologist or MTs to run them. The medical technologist had the education and training for complex testing and we did not. It took several years for lab managers and pathologist to realize the hematologist knew very little if anything about how to handle tissue. It is very different from fluids and smears as we know. Many states have NSH state organizations and ways to get CEU's now and then of course the national meeting as well at teleconferences. We can do it and move forward to get the recognition we all want if we do it together and stop talking about how hard it will be. We are currently (chronically) in a shortage of histologist in all registered phases for ASCP (HT, HTL, QIHC etc) and it is getting worse. More schools have been opening for us and more ways to get registered with education. We need to tell our children in schools about Histology and how exciting and needed it is so we get more of them into our profession. Tell them we get the specimens from CSI and help them solve cases. In this day and age it is the a great way to start and any thing that gets attention use IT. I don't want the profession I have been in and loved for over 35 years to become a lab aid position because we waited too long, thought it was to hard and did not modernize the requirements when we could. Now Pearl and I have both put our feet in it so you can be angry at two of us or join in and make it better for Histology and the field we all love or we would not be reading HistoNet. Pam Marcum -------Original Message------- From: Gervaip@aol.com Date: 02/28/05 20:44:26 To: pruegg@ihctech.net; histonet@pathology.swmed.edu Subject: Re: [Histonet] ASCP - lots of questions!!! >From what I read, those newly registered will be required to have CEUs in order to renew their registry with the ASCP. I have not ready anything that is going to require those that have been registered to acquire CEUs. I have thought that eventually we will all be required to have the CEUs in order to renew membership. Why did the ASCP not do that? I think it is a good idea. But what will happen if one does not renew their registry? What kind of regulations are going to require that the histologists renew their membership? How are they going to enforce this? And if it turns out that this will be required of all of us ... what is to keep labs from hiring unregistered (unrenewed membership). Is this really going to amount to anymore then a pile of beans? It would be nice if it did. As it is, there are some pathologists that feel a monkey can crank that microtome. No respect for our professions. This is really a true statement. Here in the state of Louisiana when Licensure came up for medical professionals, we were not included. Intentionally not included. Not just an oversight. Manicurist and hair dressers here are licensed, but not the histotechnologist! And some of this disrespect has been earned! It has taken so long for us to accept the fact that we need college. How many of us out there can say that we have kept up with new technology in our field? If you are a member of this histonet server and an NSH member you most likely have taken those giant steps forward. Think of all the folks you know in histology that think they know it all and yet you would not want them in your lab! Maybe if we do this and bit that bullet, get our education (four years of college) and CEUs CLIA might recognize us! CLIA might just realize that we are a professional group that can do "complicated" tests, such as flow, fish and probes. If they knew how complicated immunohistochemistry is, they just might take that away from us too! If they knew that we are the professionals that make that final diagnosis they might not let us work in the lab! They can do all the blood work and do all kinds of tests.... but they still need us! My apologies,if I have offended anyone. My cage just got rattled and I just can't be quiet anymore. Pearl, from Louisiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Tue Mar 1 09:04:51 2005 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] ASCP - lots of questions!!! In-Reply-To: References: Message-ID: <42248493.3030608@bitstream.net> I have been out of the laboratory for some time (well actually... a very LONG time), so please be patient and understanding. Am I to understand from Pearl's missive that the ASCP is requiring CEUs in order to "renew" your Registry?? For the sake of clarity here, when you say "Registry" are you talking about the Big Test that the ASCP proctors and, if you pass it, then you are "Registered" as an ASCP -- HT/HLT/CT/MLA/MT/etc.? If this what is being discussed, then why is the ASCP requiring that you to maintain CEUs in order to "renew" your Registry? This was a big issue with Med Techs in the late 60's and 70's. In order to keep your "Registry" with ASCP, we had to send in our annual membership dues and receive a (not to attractive) "postage stamp" that we affixed to our Registry Certificate. (Anyone out there remember those little stamps??). If we did not send in our annual dues, we would be dropped from "The Registry". Then some one got wise and said "Wait a minute!". By taking and passing the ASCP Registry Exam, we had proven that we had reached a certain level of competence that is represented by the Certificate that was awarded to us by the certifying body (i.e. ASCP). It is no different than meeting all the requirements that are necessary to receive a college degree. To prove that you had met all of the requirements you, were awarded a Diploma that stated this fact from the College or University that set up this accreditation. But no one was required to send in annual dues to that College or University in order to keep that Diploma alive. Once you had it, ...you had it! It could not be taken away. The Med Tech Society took the ASCP to task over this and they dropped the annual "renewal dues" requirement. Now, however, depending on where we worked and how our lab was accredited, we WERE required to maintain CEUs in order to keep a license or an accreditation. But CEUs were not required to maintain our "Registry", for reasons already mentioned. Am I reading this thread correctly, or am I way out in space again? ~ Ford Ford M. Royer, MT(ASCP) Midwest Science & Biocenter Minneapolis, MN (800) 745-4869 Gervaip@aol.com wrote: >>From what I read, those newly registered will be required to have CEUs in >order to renew their registry with the ASCP. I have not ready anything that >is going to require those that have been registered to acquire CEUs. I have >thought that eventually we will all be required to have the CEUs in order to >renew membership. Why did the ASCP not do that? > > I think it is a good idea. But what will happen if one does not renew >their registry? What kind of regulations are going to require that the >histologists renew their membership? How are they going to enforce this? And if >it turns out that this will be required of all of us ... what is to keep >labs from hiring unregistered (unrenewed membership). Is this really going to >amount to anymore then a pile of beans? It would be nice if it did. > As it is, there are some pathologists that feel a monkey can crank that >microtome. No respect for our professions. This is really a true >statement. Here in the state of Louisiana when Licensure came up for medical >professionals, we were not included. Intentionally not included. Not just an >oversight. Manicurist and hair dressers here are licensed, but not the >histotechnologist! >And some of this disrespect has been earned! It has taken so long for us to >accept the fact that we need college. How many of us out there can say that >we have kept up with new technology in our field? If you are a member of >this histonet server and an NSH member you most likely have taken those giant >steps forward. Think of all the folks you know in histology that think they >know it all and yet you would not want them in your lab! Maybe if we do this >and bit that bullet, get our education (four years of college) and CEUs CLIA >might recognize us! CLIA might just realize that we are a professional group >that can do "complicated" tests, such as flow, fish and probes. If they >knew how complicated immunohistochemistry is, they just might take that away >from us too! If they knew that we are the professionals that make that final >diagnosis they might not let us work in the lab! They can do all the blood >work and do all kinds of tests.... but they still need us! > My apologies,if I have offended anyone. My cage just got rattled and I >just can't be quiet anymore. > >Pearl, from Louisiana >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From pex0220 <@t> yahoo.com.cn Tue Mar 1 09:27:15 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] bone tissue section Message-ID: <20050301152715.35275.qmail@web15504.mail.cnb.yahoo.com> Hi, histonetter I am doing immunoflourescence in bone tissue. but I have some difficulties in doing it. during the experiment, I find that bone tissue often drops from slides, I use the superfrost slide, which is better. so I do not know why this condition appears. Can someone give me some advice?Thank you! Qian --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From gcallis <@t> montana.edu Tue Mar 1 09:52:13 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Re: Flying brains Message-ID: <6.0.0.22.1.20050301083747.01b50e80@gemini.msu.montana.edu> Although breathing gently into a cryostat chamber may help solve the problem, this is also an aerosol although more gentle than a spray - neither is ideal when working with potential biohazards. Obviously warm moist air is solving the problem. We experience the "flying section syndrome" in our lack of humidity climate, and it is worse when using colder sectioning temperatures. Maybe a warm water dampened gauze sitting inside the chamber, or moisten back of gloves. Dryer sheets to wipe down metal parts, antiroll plates, knife holders, and gloves. We cut rodent brain at -16C. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Tue Mar 1 10:14:37 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] xylene free mounting media Message-ID: <6.0.0.22.1.20050301085914.01b50ff0@gemini.msu.montana.edu> The mounting media we purchase contains toluene. It is compatible with xylene substitutes, Clearite 3 and Propar and is commonly found as the solvent for MANY mountants. Just check the MSDS provided by companies before buying the media. If she is sensitive to xylene, be aware that some xylene substitutes are sensitizing, limonene (orange juice smelling stuff) has also been a problem. Hopefully your technician is wearing nitrile gloves, changes these frequently, and works at proper distance inside a hood during coverslipping, and stripping off gloves inside the hood. Mounting coverslips inside a downdraft hood which pulls solvent fumes down rather than up (towards) the face is a handy device. If the hood has a filter rather than vented to outside, be sure your filters are changed out correctly when saturated. Years ago, I saw one person so allergic to xylene almost pass out when exposed to these fumes drifting via poor venting from an exhibition room into a lecture room. A near disaster since he was the workshop lecturer. Vendors, thankfully, no longer exhibit with solvents in the tanks!! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From pex0220 <@t> yahoo.com.cn Tue Mar 1 11:27:05 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Cutting bone tissue sections Message-ID: <20050301172705.1486.qmail@web15505.mail.cnb.yahoo.com> I find that it is not easy to cut bone tissue section. If you have some experience, I hope that you can give me some advice! Thank you very much! Qian --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From mprice26 <@t> juno.com Tue Mar 1 11:31:30 2005 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] RE:Blade Holder for a Shandon AS325 Retractive Microtome Message-ID: <20050301.093213.860.13414@webmail35.nyc.untd.com> Histonetters, I am searching for a Blade Holder for an Shandon AS325 Retractive Microtome blade holder. Does anyone know where I might find one? Mine has a bick in the front part of the blade holder and causes a groove in every block I face into. Thank you. Marsha Price From John.Auld <@t> whnt.nhs.uk Tue Mar 1 11:41:57 2005 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Re: Histonet Digest, Vol 15, Issue 39 Message-ID: Hi Jean 34BE12 is notorious for inconsistency, I have previously used it with a mixture of Heat and trypsin (20 minutes MW in Citrate @ pH 6.0 followed by 15 seconds trypsin be careful not to overdo the trypsin as it can overdigest in a very few seconds. Cytokeratin 5/6 has been found to give more consistant results. Hope this is of some help John John Auld MSc CSci FIBMS BMS 4 Dept of Histopathology and Clinical Cytology Arrowe Park Hospital Arrowe Park Road Upton Wirral External Tel 0151 604 7025 Message: 13 Date: Fri, 25 Feb 2005 11:02:57 -0000 From: "Jean Gillson" Subject: [Histonet] 34BE12 on prostate[Scanned] To: Message-ID: <1030B679AD69D6119C3F00080210DD9D05AC0348@bhrv-nt-11.bhrv.nwest.nhs.uk> Content-Type: text/plain; charset="iso-8859-1" Dear all fellow histologist, We seem to have a recurrent problem with 34BE12 IHC staining on both prostate chippings and prostatectomy specimens. Some basal cells stain whilst others that should stain are negative. Our procedure is Trypsin for 12 minute at pH 7.8 and 37 degrees C. We use a Dako autostainer machine and our antibody is from Dako. Previous spec sheets state Trypsinisation but our most recent one states heat retrieval. So as a test we did both retrieval methods. Oddly enough, microwaved in Dako retrieval solution was slightly better in prostatectomy specimens than trypsin (still uneven staining) and worst in chipping specimens. Can anyone offer any advice? What antibody panels are people doing for prostate cases? Help desperately needed!! Jean Gillson BMS2 Histology Department Blackburn Royal Infirmary Blackburn UK From ploykasek <@t> phenopath.com Tue Mar 1 11:55:08 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] ASCP - lots of questions!!! In-Reply-To: Message-ID: Pearl, I do agree with most of your statement. Was just wondering what final diagnosis are you making? Histotechs can do FISH but NO ONE but a doc should interpret FISH on solid tumors - histo techs, med techs and cytogenetics techs do not have the training to determine what is tumor and what is not tumor. This is a regulation. For anatomic pathology, the CLIA & CAP etc consider the docs ?testing personnel? - they are the ones making the final diagnosis. We are here to perform technological services to ensure high quality patient care, and to support the pathologist. As I have moved along in my career, I have come to view my role a bit more as a ?support? for the docs. Without our high quality lab work, the docs can not make as good a diagnosis. I try to work with the docs to come up with the services to meet their needs. And if you?re really lucky, as I have been, you work for people who value your skills and knowledge. Just my thoughts on this rather interesting topic. Patti Loykasek PhenoPath Laboratories Seattle, WA \ From what I read, those newly registered will be required to have CEUs in > order to renew their registry with the ASCP. I have not ready anything that > is going to require those that have been registered to acquire CEUs. I have > thought that eventually we will all be required to have the CEUs in order to > renew membership. Why did the ASCP not do that? > > I think it is a good idea. But what will happen if one does not renew > their registry? What kind of regulations are going to require that the > histologists renew their membership? How are they going to enforce this? > And if > it turns out that this will be required of all of us ... what is to keep > labs from hiring unregistered (unrenewed membership). Is this really going > to > amount to anymore then a pile of beans? It would be nice if it did. > As it is, there are some pathologists that feel a monkey can crank that > microtome. No respect for our professions. This is really a true > statement. Here in the state of Louisiana when Licensure came up for medical > professionals, we were not included. Intentionally not included. Not just > an > oversight. Manicurist and hair dressers here are licensed, but not the > histotechnologist! > And some of this disrespect has been earned! It has taken so long for us to > accept the fact that we need college. How many of us out there can say that > we have kept up with new technology in our field? If you are a member of > this histonet server and an NSH member you most likely have taken those giant > steps forward. Think of all the folks you know in histology that think they > know it all and yet you would not want them in your lab! Maybe if we do this > and bit that bullet, get our education (four years of college) and CEUs CLIA > might recognize us! CLIA might just realize that we are a professional group > that can do "complicated" tests, such as flow, fish and probes. If they > knew how complicated immunohistochemistry is, they just might take that away > from us too! If they knew that we are the professionals that make that final > diagnosis they might not let us work in the lab! They can do all the blood > work and do all kinds of tests.... but they still need us! > My apologies,if I have offended anyone. My cage just got rattled and I > just can't be quiet anymore. > > Pearl, from Louisiana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Edmondson <@t> christie-tr.nwest.nhs.uk Tue Mar 1 11:53:54 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Help Message-ID: Hi, Did you find an answer, or ewven THE answer. We went on to use EDTA to make sure of the immuno looking good. Maybe the 15% Formic gave more "background" or non-specific staining. Dave Histology Christie Hosp Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Traphagan, Patricia Sent: 22 February 2005 18:05 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help We are looking for a formic acid decalcifier for our bone marrows. We have been told that this type of decalcifier works best on bone marrows when a Kappa and Lambda IP stain is requested. We have tried the BBC formic acid decalcifier, but found that the length of time it needed to be in the decal was long (up to 4 fours) and the core was still calcified. Are there any suggest on what could work? Thanks PRIVACY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain business confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If this e-mail was not intended for you, please notify the sender by reply e-mail that you received this in error. Destroy all copies of the original message and attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************* This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* From Bauer.Karen <@t> mayo.edu Tue Mar 1 12:01:31 2005 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Hematoxylin Message-ID: Hello, I was just wondering what everyone is using for Hematoxylin these days. We are one of those rare Histology Labs that makes their own hematoxylin and eosin and I just got the OK from our Pathologist to try out some commercial brands. What do you like? What are you using? Good and bad comments are greatly appreciated. Thanks!! Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From histobabs <@t> hotmail.com Tue Mar 1 12:26:24 2005 From: histobabs <@t> hotmail.com (B T) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] antibody diluting buffer Message-ID: Hello all, Apparantly Dakocytomation no longer has the antibody diluting buffer available that we have been using for many years. This is truely unfortunate as we have found it to be extremely stable over long periods of time. The new product they are forced to offer is not very stable, and unsuitable for our purposes. I know other companies must offer a similar product, so I am interested in what other people are using, from what company, and how long it seems to remain stable. Thanks so much for your input. Barb From janick.jean-marie <@t> laposte.net Tue Mar 1 12:30:02 2005 From: janick.jean-marie <@t> laposte.net (janick.jean-marie) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] double immunofluorescence in mammary gland tissue Message-ID: Hi fellows, I have some problems to make an double immunofluorescence with paraffin embedded tissue sections. I suppose, my problem is the protocol of Deparaffinize sections the bath of xylen and hydratation protocol, who may mask the antigenic determinants Have you got a protocol for help me ?... I think to do a treatment of tissue section for unmasked the antigenic determinants ... Have you got a good protocol for this process? THANKS !!! Janick JEAN-MARIE CRLC INSERM Acc?dez au courrier ?lectronique de La Poste : www.laposte.net ; 3615 LAPOSTENET (0,34?/mn) ; t?l : 08 92 68 13 50 (0,34?/mn) From Diane.Gladney <@t> se.amedd.army.mil Tue Mar 1 12:38:17 2005 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Hematoxylin Message-ID: <4D55B2E997EFAE4DA6081DDE100B830237C48D@amedmlsermc133.amed.ds.army.mil> We use Richard-Allan Scientific Hematoxylin 2, Clarifier 2, Bluing Reagent and Eosin w/ Phloxine. Our docs really like these stains. We have an auto tissue slide stainer. I even use the Hematoxylin, Bluing and Eosin w/ Phloxine for our frozen sections ("quick H & E"). Hope this helps. Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology/Cytology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart Avenue P.O. Box 484 Ft. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen Sent: Tuesday, March 01, 2005 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin Hello, I was just wondering what everyone is using for Hematoxylin these days. We are one of those rare Histology Labs that makes their own hematoxylin and eosin and I just got the OK from our Pathologist to try out some commercial brands. What do you like? What are you using? Good and bad comments are greatly appreciated. Thanks!! Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Mar 1 12:39:54 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Re:Hematoxylin In-Reply-To: References: Message-ID: <6.0.0.22.1.20050301113404.01b6a250@gemini.msu.montana.edu> Karen, Contact your Richard Allen sales rep, and have them send smaples of all the different types of hematoxylins (Gill formulations, Harris, progressive hematoxylins, and Mayers) or the just ones you want to try. We prefer hematoxylin 1, a progressive hematoxylin that does not need acid alcohol differentiation. It can be shipped to Montana in winter without freezing. Richard Allen has a wonderful hematoxylin and eosin staining guide, found on their website to help you out with any of the various types. WE never went wrong with Gill II formulations either. They also sell eosin y and other reagents needed for hematoxylin staining, clarifier (acetic acid solution) and bluing solution. Good luck At 11:01 AM 3/1/2005, you wrote: >Hello, > >I was just wondering what everyone is using for Hematoxylin these days. >We are one of those rare Histology Labs that makes their own hematoxylin >and eosin and I just got the OK from our Pathologist to try out some >commercial brands. What do you like? What are you using? Good and bad >comments are greatly appreciated. > >Thanks!! > >Karen Bauer HT(ASCP) >Histology Supervisor >Luther Hospital >Eau Claire, WI >********************Confidentiality Notice******************** > > > >This message is intended for the sole use of the individual and entity to >whom it is addressed, and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. Any >unauthorized review, use, disclosure or distribution of this email >message, including any attachment, is prohibited. If you are not the >intended recipient, please advise the sender by reply email and destroy >all copies of the original message. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From BriggsK <@t> drmc.drhsi.org Tue Mar 1 12:48:20 2005 From: BriggsK <@t> drmc.drhsi.org (Kevin Briggs) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] CAP Question Message-ID: <7C1D5BE1ADF06E488AEB7A6247214A86015FB2F4@HORNET.drmc.drhsi.org> Hello Netters, I am probing the pro's to get examples of how other sites are handling the following CAP checklist item: ANP.22925 Phase I For immunohistochemistry tests that provide independent predictive/prognostic information, does the patient report include information on specimen processing, the antibody clone, and the scoring method used? Thanks, Kevin D. Briggs, MS, CT(ASCP) Team Leader- Cytopathology/ Histopathology Services Danville Regional Medical Center 142 South Main Street Danville, VA 24541 Telephone: (434) 799-4470 ext.5451 Fax: (434) 799-2118 E-mail: briggsk@drhsi.org From hymclab <@t> hyhc.com Tue Mar 1 12:55:30 2005 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Hematoxylin Message-ID: Karen, We use Surgipath's Hematoxylin and Eosin. They are the best we have found and coincidently the cheapest also. Hope this helps. Dawn Schnedier HT(ASCP) Howard Young Medical Center Woodruff, WI -----Original Message----- From: Bauer, Karen [mailto:Bauer.Karen@mayo.edu] Sent: Tuesday, March 01, 2005 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin Hello, I was just wondering what everyone is using for Hematoxylin these days. We are one of those rare Histology Labs that makes their own hematoxylin and eosin and I just got the OK from our Pathologist to try out some commercial brands. What do you like? What are you using? Good and bad comments are greatly appreciated. Thanks!! Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carolb <@t> mail.phys.mcw.edu Tue Mar 1 12:55:38 2005 From: carolb <@t> mail.phys.mcw.edu (Carol Bobrowitz) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Dako Antibody Diluent Message-ID: <59DF6640CDC0D7119DF000D0B761393C1A9646@thor.phys.mcw.edu> I just spoke to Dako and the antibody diluents. Catalog #'s S-0809 Antibody Diluent and S-3022 Antibody Diluent with Background Reducing Components are still available and will be available. Nothing with these two items are changing. What antibody diluent are you speaking about? Thank you, Carol Ann Bobrowitz Histology Laboratory Department of Physiology - Room 541 Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, Wisconsin 53226 414-456-8179 FAX 414-456-6546 From KMH.02 <@t> ex.uchs.org Tue Mar 1 13:12:55 2005 From: KMH.02 <@t> ex.uchs.org (Hopkins, Karen) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] RE: disinfection of cryostat Message-ID: <640B23A5DC4B234BB065E56F2DB30596D9628B@uchex2ucmc.uchs.org> Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... From TJJ <@t> Stowers-Institute.org Tue Mar 1 13:15:40 2005 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] ASCP - lots of questions!!! Message-ID: I have not paid my annual ASCP dues for almost 10 years now. Perhaps until recently ( I wouldn't know because I no longer receive the publication) there were never any articles which had any application toward Histotechnology in the Lab Medicine journal. All the ASCP symposiums never had one workshop that pertained to Histotechnology. I sent in my last renewal in 1996 with a note complaining about this fact. I got no response and saw no changes about this. I decided then it was in my best interest for continuing education to focus instead on my local and national societies as they could best provide me with information I needed to be my best. I am HT(ASCP) certified whether I have my sticker or not; been there, done that, passed the test. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From histopath <@t> cvm.tamu.edu Tue Mar 1 13:27:41 2005 From: histopath <@t> cvm.tamu.edu (Histopath) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] ASCP Message-ID: I have a question about the certification also. If one were to get certified this year and leave the industry for a period of time, say to raise children, she or he would not be able to go back to histology? Without an employer to help with the cost of CEU many might not be able to keep their certification. As mentioned in another email you do not "lose" your BS degree from an accredited university. From ndumont <@t> neurochem.com Tue Mar 1 13:51:49 2005 From: ndumont <@t> neurochem.com (Dumont, Nancy) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] antibody anti mouse serum amyloid A (SAA) Message-ID: <1FAA5B74210C9A45A37A46EF6A969761076377@svrneurochem9.neurochem.local> Hi everybody! I'm a little bit desperate. Is there anybody here who has used a commercial antibody against mouse serum amyloid A (SAA) for immunohistochemistry on mouse sections? I'm looking for a supplier but so far, l couldn't find any. Or if you have a protocol working on mouse tissue with an anti-human SAA cross reacting with mouse it would be greatly appreciated. We tried many protocols but we've got nothing. Please help me!!! Nancy Dumont Neurochem Inc. Laval, Canada L'information contenue dans ce courriel est confidentielle et protegee par le secret professionnel. Elle n'est destinee qu'a l'usage du destinataire indique ci-dessus. Il est strictement interdit de distribuer ce document ou l'information qu'il contient. Si vous avez recu ce courriel par erreur, ou s'il ne vous est pas destine, veuillez le mentionner immediatement a l'expediteur et detruire tous les exemplaires de ce courriel. Merci. / This email is intended only for the party to whom it is addressed, and may contain information which is privileged or confidential. Any other disclosure is strictly prohibited. If you have received this email in error, or are not a named recipient, please notify the sender immediately and destroy all copies of this email. Thank you. From cwscouten <@t> myneurolab.com Tue Mar 1 14:04:27 2005 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] RE: disinfection of cryostat Message-ID: <5784D843593D874C93E9BADCB87342AB44F6B3@tpiserver03.Coretech-holdings.com> Why disinfect the cryostat? Let the durn thing disinfect itself. IF you have this requirement, you may want a self disinfecting cryostat. See the link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hopkins, Karen Sent: Tuesday, March 01, 2005 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disinfection of cryostat Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Tue Mar 1 14:07:15 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] antibody diluting buffer Message-ID: Hello Barb, I use DakoCytomation's Antibody Diluent # S3022. Very concerned. Which one do you use? Dana Settembre University Hospital-UMDNJ >>> B T 3/1/2005 1:26:24 PM >>> Hello all, Apparantly Dakocytomation no longer has the antibody diluting buffer available that we have been using for many years. This is truely unfortunate as we have found it to be extremely stable over long periods of time. The new product they are forced to offer is not very stable, and unsuitable for our purposes. I know other companies must offer a similar product, so I am interested in what other people are using, from what company, and how long it seems to remain stable. Thanks so much for your input. Barb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Mar 1 14:36:31 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] antibody anti mouse serum amyloid A (SAA) In-Reply-To: <1FAA5B74210C9A45A37A46EF6A969761076377@svrneurochem9.neurochem.local> Message-ID: <000001c51e9e$5a411d50$76d48a80@AMY> Nancy There is not a commercially available antibody to mouse saa. All the reference literature talk about a rabbit anti-mouse saa-1, but this antibody is not available commercially; you have to get it made. I did try an antibody to saa from abcam it was a mouse anti-human that was supposed to work in paraffin sections, but I could not get this to work in mouse tissue, my negative controls stained as positive as the antibody. I stopped working on it after awhile. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dumont, Nancy Sent: Tuesday, March 01, 2005 12:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody anti mouse serum amyloid A (SAA) Hi everybody! I'm a little bit desperate. Is there anybody here who has used a commercial antibody against mouse serum amyloid A (SAA) for immunohistochemistry on mouse sections? I'm looking for a supplier but so far, l couldn't find any. Or if you have a protocol working on mouse tissue with an anti-human SAA cross reacting with mouse it would be greatly appreciated. We tried many protocols but we've got nothing. Please help me!!! Nancy Dumont Neurochem Inc. Laval, Canada L'information contenue dans ce courriel est confidentielle et protegee par le secret professionnel. Elle n'est destinee qu'a l'usage du destinataire indique ci-dessus. Il est strictement interdit de distribuer ce document ou l'information qu'il contient. Si vous avez recu ce courriel par erreur, ou s'il ne vous est pas destine, veuillez le mentionner immediatement a l'expediteur et detruire tous les exemplaires de ce courriel. Merci. / This email is intended only for the party to whom it is addressed, and may contain information which is privileged or confidential. Any other disclosure is strictly prohibited. If you have received this email in error, or are not a named recipient, please notify the sender immediately and destroy all copies of this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HETZER <@t> surgery.wisc.edu Tue Mar 1 15:22:56 2005 From: HETZER <@t> surgery.wisc.edu (Michael Hetzer) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] disinfection of cryostat Message-ID: Use 100% ETOH to disinfect your cryostat without turning it off. We use the last of the dehydrating ETOH from the previous day's staining setup.Wear your PPE and use 4x4 gauze that is soaked with the ETOH to the point of dripping a little bit. Dispose of your blade first or if your using a knife first disifect both sides of the kife using only upward wipes and then set the knife in a safe out of the way place inside the cryostat preferrably a spot that has already been cleaned. Remove any waste materials with the wet gauze and then wipe the inner surfaces.Wipe out any excess ETOH with dry 4x4 gauze. It will take you five minutes at the most. Mike Hetzer Dermatologic Procedures Madison, WI From AnthonyH <@t> chw.edu.au Tue Mar 1 17:12:06 2005 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] disinfection of cryostat Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3741269E1CD@simba.kids> Michael, 100% ethanol is a poor disinfectant. 70% is better. The 30% water content is need for the ethanol to be reactive with the various pathogens that ethanol is effective against. Unfortunately the water content may result is ice formation but a good wipe with a dry rag could be used. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Michael Hetzer [mailto:HETZER@surgery.wisc.edu] Sent: Wednesday, 2 March 2005 8:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disinfection of cryostat Use 100% ETOH to disinfect your cryostat without turning it off. We use the last of the dehydrating ETOH from the previous day's staining setup.Wear your PPE and use 4x4 gauze that is soaked with the ETOH to the point of dripping a little bit. Dispose of your blade first or if your using a knife first disifect both sides of the kife using only upward wipes and then set the knife in a safe out of the way place inside the cryostat preferrably a spot that has already been cleaned. Remove any waste materials with the wet gauze and then wipe the inner surfaces.Wipe out any excess ETOH with dry 4x4 gauze. It will take you five minutes at the most. Mike Hetzer Dermatologic Procedures Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From TheBestTime23 <@t> aol.com Tue Mar 1 17:24:18 2005 From: TheBestTime23 <@t> aol.com (TheBestTime23@aol.com) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] The monkey cranking the microtome Message-ID: <92.21b748a8.2f5653a2@aol.com> If I were to go back to college for 4 years my goal would be more lofty than to remain a histotech. I think there are a lot of people who do this because it pays well and it doesn't take to long to learn. If you start having requirements like college credits, people will be less likely to take interest in histotechnology as a career. They just started these requirements, but the need for histotechs is starting to slow. I've heard from older techs that the ASCP has been back and forth on their pre-reqs many times depending on the surplus or deficit of techs. I do, however, think that there is nothing wrong with the continuing education. I only wish that there were more interesting seminars or the like to go to. I went and got my first credit already (having only been registered since December) and it was on basic fixation. Nothing that I didn't already know... Just a few thoughts, Megan From victor <@t> pathology.washington.edu Tue Mar 1 17:50:11 2005 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Deeper vs Levels Message-ID: <4224FFB3.5000003@pathology.washington.edu> We have this on going issue of the use of the terms such as deeper vs levels. For this example let's use 3 levels vs 3 deeper sections. There is no question regarding the understanding of 3 levels, but what about 3 deeper sections? My immediate boss says 3 deeper sections are essentially 3 recuts at one deeper level. In talking with some of the techs, they would treat 3 deeper sections the same as 3 levels. We want to clean-up our database and eliminate any confusion. Pathologist thinks they are ordering this and get that. Any comments? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From conniemoss <@t> relia.net Tue Mar 1 18:19:42 2005 From: conniemoss <@t> relia.net (Connie McManus) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] ASCP In-Reply-To: References: Message-ID: <49547.208.186.240.165.1109722782.squirrel@email.relia.net> I'm a bit lost in this thread, but from HISTOPATH's querry, I'm understanding her/him to mean that new regs from ASCP will require CEU's in order to retain the registry. I think CEU's are a good idea even if you're working in the field. However, my experiece has been a bit different. I had a hiatus from histo for a period of about 10 years. However, I also had about 10 yrs working in the field prior to that. During that hiatus period, I learned EM, got my BS in biology, worked as a microbiologist, learned tissue culture/virology, then came back to histology. During my hiatus, I kept my ASCP registration. I paid my dues every year, even when $$ were hard to come by. By reading the Laboraory Medicine journals that came to me, I was able to keep informed. I wish I had had the internet in those days!!! I firmly believe that anyone can do anything if they have the desire to do it. All they need is the opportunity and someone to support them/believe in them. Many times in my career as a laboratorian, I have been the recipient of both. What this means for someone who takes a hiatus for whatever reason is -- during your time out of the workplace, keep abreast of the technology in the field. This can be as simple staying tuned to Histonet and visiting other internet sites. Wouldn't it be a coold idea for the NSH or ASCP to provide some way for people on hiatus who want to keep current to take the CEU's by some sort of deferred payment plan or a loan or something until they successfully return to work??? Or is there already such a plan in place??? Just rambling off the top of my head. *g* -- Connie McManus, HT Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com ====== Histopath said: > I have a question about the certification also. If one were to get > certified this year and leave the industry for a period of time, say to > raise children, she or he would not be able to go back to histology? > Without an employer to help with the cost of CEU many might not be able > to keep their certification. As mentioned in another email you do > not "lose" your BS degree from an accredited university. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From conniemoss <@t> relia.net Tue Mar 1 18:32:01 2005 From: conniemoss <@t> relia.net (Connie McManus) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Deeper vs Levels In-Reply-To: <4224FFB3.5000003@pathology.washington.edu> References: <4224FFB3.5000003@pathology.washington.edu> Message-ID: <49575.208.186.240.165.1109723521.squirrel@email.relia.net> Victor, Stepping out on a limb, here... in my mind, "deeper" is just what the doc said, it's a recut. "Levels", on the other hand, implies a specified depth ... 10 um, 15 um, whatever, per level. I would think that whatever depth defines a "level" would be part of the way things are done in the lab (there's a term I'm looking for and it escapes me at the moment...grrr ... old age). this is an interesting question, one I've never delt with before. anyway, that's my thinking on it, for what it's worth. -- Connie McManus, HT Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com ====== Victor Tobias said: > We have this on going issue of the use of the terms such as deeper vs > levels. For this example let's use 3 levels vs 3 deeper sections. There > is no question regarding the understanding of 3 levels, but what about 3 > deeper sections? My immediate boss says 3 deeper sections are > essentially 3 recuts at one deeper level. In talking with some of the > techs, they would treat 3 deeper sections the same as 3 levels. We want > to clean-up our database and eliminate any confusion. Pathologist thinks > they are ordering this and get that. Any comments? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histology.bc <@t> shaw.ca Tue Mar 1 19:55:29 2005 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] The monkey cranking the microtome In-Reply-To: <92.21b748a8.2f5653a2@aol.com> References: <92.21b748a8.2f5653a2@aol.com> Message-ID: <42251D11.7080308@shaw.ca> Ooohh! I am sure that this will not be the only response you get to your e-mail !! For someone who has been qualified only since December, you are taking a very condescending attitude toward a profession that you obviously do not fully appreciate. If your only experience of histotechnology is that of section cutting and mass producing sections, you have overlooked major portions of the responsibilities of being a technologist. I am sure there are some workers out there who do "just crank the microtome" and who do not have any other career ambitions. These are the same people who describe themselves as "just a histotech", as though it were something to be ashamed of. If this is truly your attitude, I would suggest that you find another profession ... and quickly. The difference between "just a histotech" and a true histotechnologist is the same as the difference between an unmotivated short-order cook flipping hamburgers and a well educated chef preparing a gourmet feast. To the "just a histotech", it is just a job, a way of making few bucks without having to expend much effort. To the true professional, it is a career that requires a sold background in chemistry, biology, immunology, and physics, along with a wide range of experience. A well-educated histotechnologist is a vital member of the diagnostic team, without his/her participation and knowledge, an accurate diagnosis will not be possible. A diagnostic pathology laboratory can only be as good as the technologists who work in it. If they are minimally trained and unmotivated, they will not be appreciated, and the standards of the laboratory willl reflect that. If they are well-educated, knowledgeable, and enthusiastic about their profession, they will be respected by the other members of the diagnostic teams, and their advice will be sought in determining the best procedures for diagnosis. In the few months since your qualified, have you had chance to do any immunohistochemistry, frozen sections, renal biopsies, lymphoma studies, muscle biopsies, histochemistry or immunofluorescence? These are the skills required of a qualified technologist ... these are the investigations that can determine the future course of a patient's life. The success of any endeavour, whether it is a career, a sport, or a relationship, will be proportional to the effort that is put into it. If you are not willing to put effort and dedication into this career, you will not achieve any level of satisfaction ... and you will fail. Go to the nest NSH meeting, I defy you to say that "there was nothing I did not already know". You will gain a new (obviously much needed) insight into the profession of histotechnology. Paul Bradbury Kamloops, BC From cforster <@t> umn.edu Tue Mar 1 20:55:08 2005 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] (no subject) Message-ID: <42252B0C.1080905@umn.edu> Hats off to you Paul, well said. I agree with you completely. Our "profession" is a very specialized and demading one that requires the proper education and training. I wonder what part of the country is NOT in need of good histotechs? Not mine for sure. Colleen Forster HT(ASCP)QIHC University of Minnesota Division of Neuropathology From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 2 01:49:17 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] Hematoxylin[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F032@bhrv-nt-11.bhrv.nwest.nhs.uk> Good girl.... Gone are the days when I had 5 litre flasks of Harris's haematoxylin ripening in the Stoke-on-Trent sunshine; I felt like an alchemist. Then it was plagiarised 'Gills' haematoxylin ripening, then some infernal lead haematoxylin concoction that turned everything it touched black; I strangely put on weight too. The ripening of haematoxylin to haematin then to oxyhaematin was a delight to witness, the latter meant you have gone too far. The ability to carry out an H&E progressively without having to remove the cytoplasmic 'overstaining' a delight. I'm one for keeping 'home made' haematoxylin together with retaining the 'a' in haematin, but on both points alas I feel I shall fail. -----Original Message----- From: Bauer, Karen [mailto:Bauer.Karen@mayo.edu] Sent: 01 March 2005 18:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin[Scanned] Hello, I was just wondering what everyone is using for Hematoxylin these days. We are one of those rare Histology Labs that makes their own hematoxylin and eosin and I just got the OK from our Pathologist to try out some commercial brands. What do you like? What are you using? Good and bad comments are greatly appreciated. Thanks!! Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 2 01:50:11 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] RE: disinfection of cryostat[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F033@bhrv-nt-11.bhrv.nwest.nhs.uk> Do bugs grow at minus 20? -----Original Message----- From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: 01 March 2005 20:04 To: Hopkins, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Why disinfect the cryostat? Let the durn thing disinfect itself. IF you have this requirement, you may want a self disinfecting cryostat. See the link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=47510 2&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats &idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hopkins, Karen Sent: Tuesday, March 01, 2005 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disinfection of cryostat Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 2 01:55:45 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] The monkey cranking the microtome[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F035@bhrv-nt-11.bhrv.nwest.nhs.uk> Here, here -----Original Message----- From: Paul Bradbury [mailto:histology.bc@shaw.ca] Sent: 02 March 2005 01:55 To: TheBestTime23@aol.com; HistoNet Server Subject: Re: [Histonet] The monkey cranking the microtome[Scanned] Ooohh! I am sure that this will not be the only response you get to your e-mail !! For someone who has been qualified only since December, you are taking a very condescending attitude toward a profession that you obviously do not fully appreciate. If your only experience of histotechnology is that of section cutting and mass producing sections, you have overlooked major portions of the responsibilities of being a technologist. I am sure there are some workers out there who do "just crank the microtome" and who do not have any other career ambitions. These are the same people who describe themselves as "just a histotech", as though it were something to be ashamed of. If this is truly your attitude, I would suggest that you find another profession ... and quickly. The difference between "just a histotech" and a true histotechnologist is the same as the difference between an unmotivated short-order cook flipping hamburgers and a well educated chef preparing a gourmet feast. To the "just a histotech", it is just a job, a way of making few bucks without having to expend much effort. To the true professional, it is a career that requires a sold background in chemistry, biology, immunology, and physics, along with a wide range of experience. A well-educated histotechnologist is a vital member of the diagnostic team, without his/her participation and knowledge, an accurate diagnosis will not be possible. A diagnostic pathology laboratory can only be as good as the technologists who work in it. If they are minimally trained and unmotivated, they will not be appreciated, and the standards of the laboratory willl reflect that. If they are well-educated, knowledgeable, and enthusiastic about their profession, they will be respected by the other members of the diagnostic teams, and their advice will be sought in determining the best procedures for diagnosis. In the few months since your qualified, have you had chance to do any immunohistochemistry, frozen sections, renal biopsies, lymphoma studies, muscle biopsies, histochemistry or immunofluorescence? These are the skills required of a qualified technologist ... these are the investigations that can determine the future course of a patient's life. The success of any endeavour, whether it is a career, a sport, or a relationship, will be proportional to the effort that is put into it. If you are not willing to put effort and dedication into this career, you will not achieve any level of satisfaction ... and you will fail. Go to the nest NSH meeting, I defy you to say that "there was nothing I did not already know". You will gain a new (obviously much needed) insight into the profession of histotechnology. Paul Bradbury Kamloops, BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 2 01:59:13 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] The monkey cranking the microtome[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F036@bhrv-nt-11.bhrv.nwest.nhs.uk> Then Megan, organise meetings, seminars and workshops yourself. It's very easy to become demotivated and expect someone else to get you out of the doldrums. Self motivation is a trait not found too often nowadays I find, but then I'm just old. -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: 01 March 2005 23:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] The monkey cranking the microtome[Scanned] If I were to go back to college for 4 years my goal would be more lofty than to remain a histotech. I think there are a lot of people who do this because it pays well and it doesn't take to long to learn. If you start having requirements like college credits, people will be less likely to take interest in histotechnology as a career. They just started these requirements, but the need for histotechs is starting to slow. I've heard from older techs that the ASCP has been back and forth on their pre-reqs many times depending on the surplus or deficit of techs. I do, however, think that there is nothing wrong with the continuing education. I only wish that there were more interesting seminars or the like to go to. I went and got my first credit already (having only been registered since December) and it was on basic fixation. Nothing that I didn't already know... Just a few thoughts, Megan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Wed Mar 2 03:44:31 2005 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] The monkey cranking the microtome. Message-ID: <6.1.2.0.1.20050302094120.022b1520@ucdf.gla.ac.uk> So, we have someone who knows all about fixation. I'm really glad about that as after 38 years and 6 months I'm still learning. Ian. From marytedo <@t> hotmail.com Wed Mar 2 04:18:04 2005 From: marytedo <@t> hotmail.com (María Teresa Domínguez) Date: Fri Sep 16 15:24:41 2005 Subject: [Histonet] disinfection of cryostat In-Reply-To: Message-ID: I usualy use a gauze soaked with a bleach solution (5%) to clean the cryostat,but I turned it off first, and take it off the microtome unit to clean it outside and change the knife. All this process takes half an our or less. Then you can turn the cryostat on to be used again. HT. MARIA TERESA DOMINGUEZ ANATOMIC PATOLOGY SERVICE REG. HOSPITAL OF RIO GRANDE, TIERRA DEL FUEGO, ARGENTINA. >From: "Michael Hetzer" >To: >Subject: [Histonet] disinfection of cryostat >Date: Tue, 01 Mar 2005 15:22:56 -0600 > >Use 100% ETOH to disinfect your cryostat without turning it off. We use >the last of the dehydrating ETOH from the previous day's staining >setup.Wear your PPE and use 4x4 gauze that is soaked with the ETOH to >the point of dripping a little bit. Dispose of your blade first or if >your using a knife first disifect both sides of the kife using only >upward wipes and then set the knife in a safe out of the way place >inside the cryostat preferrably a spot that has already been cleaned. >Remove any waste materials with the wet gauze and then wipe the inner >surfaces.Wipe out any excess ETOH with dry 4x4 gauze. It will take you >five minutes at the most. > >Mike Hetzer >Dermatologic Procedures >Madison, WI > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Express yourself instantly with MSN Messenger! [1]MSN Messenger Download today it's FREE! References 1. http://g.msn.com/8HMAEN/2728??PS=47575 From Jackie.O'Connor <@t> abbott.com Wed Mar 2 06:47:57 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] RE: disinfection of cryostat[Scanned] Message-ID: No - but they don't die, either - so when you breathe them into your nice warm lungs - voila! They wake up and infect you. Jacqueline M. O'Connor HT(ASCP) Assistant Scientist Cancer Research Jackie.OConnor@abbott.com Kemlo Rogerson Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2005 01:50 AM To: "'Charles Scouten'" cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Do bugs grow at minus 20? -----Original Message----- From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: 01 March 2005 20:04 To: Hopkins, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Why disinfect the cryostat? Let the durn thing disinfect itself. IF you have this requirement, you may want a self disinfecting cryostat. See the link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=47510 2&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats &idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hopkins, Karen Sent: Tuesday, March 01, 2005 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disinfection of cryostat Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Edmondson <@t> christie-tr.nwest.nhs.uk Wed Mar 2 06:47:18 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] antibody diluting buffer Message-ID: DAKO's 2022 is still available over here, and working fine Dave Christie Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of B T Sent: 01 March 2005 18:26 To: histonet@pathology.swmed.edu Subject: [Histonet] antibody diluting buffer Hello all, Apparantly Dakocytomation no longer has the antibody diluting buffer available that we have been using for many years. This is truely unfortunate as we have found it to be extremely stable over long periods of time. The new product they are forced to offer is not very stable, and unsuitable for our purposes. I know other companies must offer a similar product, so I am interested in what other people are using, from what company, and how long it seems to remain stable. Thanks so much for your input. Barb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Wed Mar 2 06:56:17 2005 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] The Histology Career Message-ID: <590F08A18A72AF46917BF721E6476B5C226BAF@EXCHANGE2.ad.uams.edu> It has been very interesting reading all of the opinions about the career of histotechnology. I have decided to put my two cents in. I have been a Histology Technician for 40 years. I became certified in 1969 but prior to that I worked as a histology technician. I learned fluorence immunostaining back then, electron microscopy, and many other techniques some of which are pass? and some are not. The main thing I learned in all of the years of being one of the most rewarding professions (in my opinion) is that we are the one of the most responsible professions in the medical community. Our profession is the only profession, that I know of, that if something happens to the specimen-it is gone. I do believe that no one really takes a look at our profession at that angle. If you had only one mole on your body and it looked like a melanoma. The doctor removed it and sent it to Pathology and something happened to that mole during the process of preparing it to the final product (i.e. the specimen got loss, the specimen was over processed, the technician cut it away, etc.) The patient's diagnosis would not be able to be confirmed nor denied. I am proud of this profession. I have learned a lot about not only the field of histotechnology but of all of the fields in the medical technical area. The mind set I am reading is alarming to me. As Ian said, after 40 years I am still learning, everyday, information about the different areas of our field. I think that the technicians in our field need to re think how they feel. If they do not like the field then find something else. Education is a very important part of this field. Everyone should take the opportunity to learn something new everyday. Remember no one can take what you learn away from you. Thanks for letting me sound off. Frances L. Swain, HT(ASCP) A. A. S. Special Procedures Technician UAMS Center for Orthopaedic Research 4301 West Markham St., Slot 644 Little Rock AR 72205 Phone: (501) 686-8789 FAX: (501) 686-8987 email: swainfrancesl@uams.edu From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 2 07:28:55 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] RE: disinfection of cryostat[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F039@bhrv-nt-11.bhrv.nwest.nhs.uk> But why did you stick your head in a cryostat? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: 02 March 2005 12:48 To: Kemlo Rogerson Cc: 'Charles Scouten'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] No - but they don't die, either - so when you breathe them into your nice warm lungs - voila! They wake up and infect you. Jacqueline M. O'Connor HT(ASCP) Assistant Scientist Cancer Research Jackie.OConnor@abbott.com Kemlo Rogerson Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2005 01:50 AM To: "'Charles Scouten'" cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Do bugs grow at minus 20? -----Original Message----- From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: 01 March 2005 20:04 To: Hopkins, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Why disinfect the cryostat? Let the durn thing disinfect itself. IF you have this requirement, you may want a self disinfecting cryostat. See the link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=47510 2&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats &idsubcategory=182 Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hopkins, Karen Sent: Tuesday, March 01, 2005 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disinfection of cryostat Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDDeltour <@t> mar.med.navy.mil Wed Mar 2 07:44:44 2005 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] ASCP testing Message-ID: <080566D001A3D9459FFC0A391A646C9104BEF84C@marxchg03.mar.med.navy.mil> What is alarming to me is that the last exam had a 75% failure percentage(so I was told). Why was there a sudden need for ASCP to make the exam harder then what it used to be (pre-2001-2002)? Is it the almighty dollar? With the new standard in play needing an Associate Degree to be eligible, how will it effect the shortage of "ASCP eligible" techs? I see many going back to school or techs being hired just for ASCP title, not experience. Will this force employers to change there hiring standards or will it be harder to find a ASCP eligible tech to fill a position? For the record I sent my blocks in in 2000 so I have until the end of the year to pass the test. It is kind of discouraging when everyone around you is failing this test 2-3 times. Do I make sense here are am I way off?? As usual :) DOUGLAS D. DELTOUR HISTOLOGY TECHNICIAN NMC PORTSMOUTH VA From Jackie.O'Connor <@t> abbott.com Wed Mar 2 07:56:55 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] RE: disinfection of cryostat[Scanned] Message-ID: Well, it was a hot day, and I had to get the Popsicles out, anyway . . . . . Scenario: You get a frozen on a lung sample to rule out tumor - you cut it - you know how frozens sometimes shatter and that stuff is all over - it's a granuloma - HEY! That might be Tuberculosis! You're hands have been in the cryostat (gloved) and what are the odds you wash your hands between cutting a section and staining it? Now you might have TB bacteria warmed up all over everything you touched outside the cryostat. It could happen. Kemlo Rogerson Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2005 07:28 AM To: "'Jackie M O'Connor'" cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] But why did you stick your head in a cryostat? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: 02 March 2005 12:48 To: Kemlo Rogerson Cc: 'Charles Scouten'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] No - but they don't die, either - so when you breathe them into your nice warm lungs - voila! They wake up and infect you. Jacqueline M. O'Connor HT(ASCP) Assistant Scientist Cancer Research Jackie.OConnor@abbott.com Kemlo Rogerson Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2005 01:50 AM To: "'Charles Scouten'" cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Do bugs grow at minus 20? -----Original Message----- From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: 01 March 2005 20:04 To: Hopkins, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Why disinfect the cryostat? Let the durn thing disinfect itself. IF you have this requirement, you may want a self disinfecting cryostat. See the link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=47510 2&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats &idsubcategory=182 Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hopkins, Karen Sent: Tuesday, March 01, 2005 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disinfection of cryostat Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Mar 2 08:03:47 2005 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Deeper vs Levels Message-ID: <6CD94D97ED7D924BA5C2B588FA95282139678E@nt_exchange.lmhealth.org> To my way of thinking 3 deeper section would be 3 cuts at one deeper level. I wouldn't think it would be that big of a deal..... Just define it to be whatever you want it to be. We had a similar problem a while back with 2 relatively new (at least to us) pathologists. I added the various terms (levels, serial, deeper, exhaust, etc.) with definitions to the bottom of our request sheet. They now know what to order to get what they want. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio 43055 -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Tuesday, March 01, 2005 6:50 PM To: Histonet Subject: [Histonet] Deeper vs Levels We have this on going issue of the use of the terms such as deeper vs levels. For this example let's use 3 levels vs 3 deeper sections. There is no question regarding the understanding of 3 levels, but what about 3 deeper sections? My immediate boss says 3 deeper sections are essentially 3 recuts at one deeper level. In talking with some of the techs, they would treat 3 deeper sections the same as 3 levels. We want to clean-up our database and eliminate any confusion. Pathologist thinks they are ordering this and get that. Any comments? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> med.nyu.edu Wed Mar 2 08:14:07 2005 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] The monkey cranking the microtome[Scanned] In-Reply-To: <1030B679AD69D6119C3F00080210DD9D05A3F036@bhrv-nt-11.bhrv.nwest.nhs.uk> Message-ID: I think everyone here has made some excellent points and I have been following this thread with great interest. I have been in this field for 18+ years and I think it is safe to say that those of us who have remained in this field for more than just a few years,.....do so because we enjoy what we do. Above all else, if your not happy with what you do, "job", "profession", "career" all the money and respect in the world will not make you happy. I tell people (residents, attending, students) I enjoy what I do very much, so much that you don't have to pay me to do it (they barely do anyway!) That being said, I think that a couple of "take home messages" from this thread are extremely important. First, no one, except histologist, can change the way this discipline is viewed by the rest of the scientific community. That community is built upon science and that is the language that we need to speak in. Second, it is up to the experienced people (I count myself as one) to pass to the Megan's of the world the challenges that we all know exist in this discipline. Be they political or scientific. Most importantly, there still are many fundamental scientific questions that need to be answered in histology. Education is the basis for answering these questions. The histology field is in a precarious position, these are not easy times we face and the tasks ahead are challenging, but as is often the case "the greater the difficulty.....the greater the reward" LC>>> -------------------------------------- Luis Chiriboga Ph.D., HT (ASCP) QIHC New York University School Of Medicine NYU Cancer Institute and Bellevue Hospital Center Department Of Pathology 4W27 27th Street & First Avenue New York, N.Y. 10016 (212) 562-4667. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo Rogerson Sent: Wednesday, March 02, 2005 2:59 AM To: 'TheBestTime23@aol.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The monkey cranking the microtome[Scanned] Then Megan, organise meetings, seminars and workshops yourself. It's very easy to become demotivated and expect someone else to get you out of the doldrums. Self motivation is a trait not found too often nowadays I find, but then I'm just old. -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: 01 March 2005 23:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] The monkey cranking the microtome[Scanned] If I were to go back to college for 4 years my goal would be more lofty than to remain a histotech. I think there are a lot of people who do this because it pays well and it doesn't take to long to learn. If you start having requirements like college credits, people will be less likely to take interest in histotechnology as a career. They just started these requirements, but the need for histotechs is starting to slow. I've heard from older techs that the ASCP has been back and forth on their pre-reqs many times depending on the surplus or deficit of techs. I do, however, think that there is nothing wrong with the continuing education. I only wish that there were more interesting seminars or the like to go to. I went and got my first credit already (having only been registered since December) and it was on basic fixation. Nothing that I didn't already know... Just a few thoughts, Megan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From herme013 <@t> umn.edu Wed Mar 2 08:11:15 2005 From: herme013 <@t> umn.edu (Yves Heremans) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] question for Dr. C. van der Loos - Permanent Red Message-ID: Dear Dr. van der Loos, I am using your book "Immunoenzyme Multiple Staining Methods" as a very usefull tool in our lab. However, I was wondering how you would categorize Dako's recently introduced Permanent Red substrate with regard to sensitivity and sharpness to the older substrates. Yves Heremans University of Minnesota Stem Cell Institute Tel 612-625-0964 Fax 612-624-2436 Address for US Postal Mail: University of Minnesota MMC 716 420 Delaware Street SE Minneapolis, MN 55455 Address for COURIER DELIVERY: Suite 14-285 Moos Tower 515 Delaware Street SE From DDDeltour <@t> mar.med.navy.mil Wed Mar 2 08:42:58 2005 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] ASCP testing Message-ID: <080566D001A3D9459FFC0A391A646C9104BEF84D@marxchg03.mar.med.navy.mil> You got me all wrong. I passed my practical back in 2000. I have 2 more times to take it before the end of the year. IF I don't pass I will be placed in the new category where I will have to have an Associates and resubmit my blocks..ect.. The discouraging thing is that many good techs will be out in the cold unless they get an Associates degree. That is if they don't pass the ASCP test. I work with many good tech that are not registered and do not have a degree. If they would want to apply for another job they could not because they would not be ASCP eligible. If there is a shortage of techs this will make it harder for employers to find ASCP eligible techs. I am all for education and knowing your job but I was just amazed that 75% of test takers failed. IS the tests and the bar being raised too high at a time where there is a shortage of techs? -----Original Message----- From: Deltour, Douglas D. (HM2) Sent: Wednesday, March 02, 2005 8:45 AM To: histonet@pathology.swmed.edu Subject: [Histonet] ASCP testing What is alarming to me is that the last exam had a 75% failure percentage(so I was told). Why was there a sudden need for ASCP to make the exam harder then what it used to be (pre-2001-2002)? Is it the almighty dollar? With the new standard in play needing an Associate Degree to be eligible, how will it effect the shortage of "ASCP eligible" techs? I see many going back to school or techs being hired just for ASCP title, not experience. Will this force employers to change there hiring standards or will it be harder to find a ASCP eligible tech to fill a position? For the record I sent my blocks in in 2000 so I have until the end of the year to pass the test. It is kind of discouraging when everyone around you is failing this test 2-3 times. Do I make sense here are am I way off?? As usual :) DOUGLAS D. DELTOUR HISTOLOGY TECHNICIAN NMC PORTSMOUTH VA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From scoop <@t> mail.nih.gov Wed Mar 2 09:01:52 2005 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] removal of carbohydrates/glycosylation Message-ID: Hi, Does anyone know of a method to remove carbohydrate modifications/glycosylation from proteins in tissue sections on slides prior to immunohistochemistry? (I know it's a weird question.) Any suggestions, references, etc. would be greatly appreciated. Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-8417 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From vazquezr <@t> ohsu.edu Wed Mar 2 09:09:33 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Deeper vs Levels Message-ID: Connie&Victor, Deepers and levels are one in the same, a recut on the other hand is "right off the top". Basically, you don't go into the block at all. Or, maybe different parts of the country call it different names...just my two cents. Robyn OHSU >>> "Connie McManus" 03/01/05 4:32 PM >>> Victor, Stepping out on a limb, here... in my mind, "deeper" is just what the doc said, it's a recut. "Levels", on the other hand, implies a specified depth ... 10 um, 15 um, whatever, per level. I would think that whatever depth defines a "level" would be part of the way things are done in the lab (there's a term I'm looking for and it escapes me at the moment...grrr ... old age). this is an interesting question, one I've never delt with before. anyway, that's my thinking on it, for what it's worth. -- Connie McManus, HT Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com ====== Victor Tobias said: > We have this on going issue of the use of the terms such as deeper vs > levels. For this example let's use 3 levels vs 3 deeper sections. There > is no question regarding the understanding of 3 levels, but what about 3 > deeper sections? My immediate boss says 3 deeper sections are > essentially 3 recuts at one deeper level. In talking with some of the > techs, they would treat 3 deeper sections the same as 3 levels. We want > to clean-up our database and eliminate any confusion. Pathologist thinks > they are ordering this and get that. Any comments? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From manuvici <@t> med.unibo.it Wed Mar 2 09:20:14 2005 From: manuvici <@t> med.unibo.it (M.Vici) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Immunostaining on bone marrow smears Message-ID: <4225E7BE.23260.188A9F7@localhost> Hi everybody! I've just joined the histonet. My question: I need to immunostain old-air dried- bone marrow smears. I'm using an anti-nucleophosmin monoclonal antibody (nucleolus). Which fixation and peroxidase suppression protocols would you suggest? Thanks! Manuela From Terry.Marshall <@t> rothgen.nhs.uk Wed Mar 2 09:40:43 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Deeper vs Levels Message-ID: This question of what are levels, deepers etc. has come up now and then through the years. In most cases the pathologist is looking for something he suspects may be there, either because of a hint of it in the original or because the clinicians are suspecting it. In a sense, it doesn't matter how you arrive at finding it, he just wants it found. There is a sub-set of that situation where the lesion is just there but very small and he wants to see it bigger and better but is frightened it may get cut through. This I personally request as "levels - shallow trim", *and* I go see the techs to ensure we are on the same wavelength. This equates to Robyn's "recut". Occasionally one may need what I call semi-serials, which is cuts at levels, but serialed at those levels. Mostly this is for affections of hair follicles, when you are just trying to get one in the plane of section. I know as a fact from previous posts that people have different terms for the same thing, and that some have a very styled rigid system for detailing what is to be done, which I think is not necessary. The bottom line is to know what the pathologist is trying to find and knowing how best to do so. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 02 March 2005 15:10 To: victor@pathology.washington.edu; conniemoss@relia.net Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Deeper vs Levels Connie&Victor, Deepers and levels are one in the same, a recut on the other hand is "right off the top". Basically, you don't go into the block at all. Or, maybe different parts of the country call it different names...just my two cents. Robyn OHSU >>> "Connie McManus" 03/01/05 4:32 PM >>> Victor, Stepping out on a limb, here... in my mind, "deeper" is just what the doc said, it's a recut. "Levels", on the other hand, implies a specified depth ... 10 um, 15 um, whatever, per level. I would think that whatever depth defines a "level" would be part of the way things are done in the lab (there's a term I'm looking for and it escapes me at the moment...grrr ... old age). this is an interesting question, one I've never delt with before. anyway, that's my thinking on it, for what it's worth. -- Connie McManus, HT Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com ====== Victor Tobias said: > We have this on going issue of the use of the terms such as deeper vs > levels. For this example let's use 3 levels vs 3 deeper sections. There > is no question regarding the understanding of 3 levels, but what about 3 > deeper sections? My immediate boss says 3 deeper sections are > essentially 3 recuts at one deeper level. In talking with some of the > techs, they would treat 3 deeper sections the same as 3 levels. We want > to clean-up our database and eliminate any confusion. Pathologist thinks > they are ordering this and get that. Any comments? > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbroomal <@t> NEMOURS.ORG Wed Mar 2 09:51:28 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] ASCP Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A7946@wlmmsx01.nemours.org> I agree... that's a good idea Connie! Just one thought from personal experience is that not every employer in every profession will pay for CEU's, even though it might be required to keep up their registration status. I'm one of those recently registered (meaning CEU's are required for me) & if my employer pays for any of my cont ed, I'll be thankful (and I think they will pay for part of it, if not most). It's not their responsibility to see that we get our CEU's, it's ours. I think that if someone wants to keep up their registry, they need to suck it up & do what they need to do in order to keep it up. I went to plenty of CE conferences for my previous profession & had to pay for most of it myself (and I was an unregistered OTJ). Even the non-OTJ people had to pay their own way most of the time. There was no requirement for me to take CE, but I wanted to keep abreast of current techniques & sometimes it was just plain fun. CE doesn't have to be like pulling teeth for goodness sakes. JMHO Kristen Broomall, HT (ASCP) -----Original Message----- From: Connie McManus [mailto:conniemoss@relia.net] Sent: Tuesday, March 01, 2005 7:20 PM To: Histopath Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP I'm a bit lost in this thread, but from HISTOPATH's querry, I'm understanding her/him to mean that new regs from ASCP will require CEU's in order to retain the registry. I think CEU's are a good idea even if you're working in the field. However, my experiece has been a bit different. I had a hiatus from histo for a period of about 10 years. However, I also had about 10 yrs working in the field prior to that. During that hiatus period, I learned EM, got my BS in biology, worked as a microbiologist, learned tissue culture/virology, then came back to histology. During my hiatus, I kept my ASCP registration. I paid my dues every year, even when $$ were hard to come by. By reading the Laboraory Medicine journals that came to me, I was able to keep informed. I wish I had had the internet in those days!!! I firmly believe that anyone can do anything if they have the desire to do it. All they need is the opportunity and someone to support them/believe in them. Many times in my career as a laboratorian, I have been the recipient of both. What this means for someone who takes a hiatus for whatever reason is -- during your time out of the workplace, keep abreast of the technology in the field. This can be as simple staying tuned to Histonet and visiting other internet sites. Wouldn't it be a coold idea for the NSH or ASCP to provide some way for people on hiatus who want to keep current to take the CEU's by some sort of deferred payment plan or a loan or something until they successfully return to work??? Or is there already such a plan in place??? Just rambling off the top of my head. *g* -- Connie McManus, HT Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com ====== Histopath said: > I have a question about the certification also. If one were to get > certified this year and leave the industry for a period of time, say to > raise children, she or he would not be able to go back to histology? > Without an employer to help with the cost of CEU many might not be able > to keep their certification. As mentioned in another email you do > not "lose" your BS degree from an accredited university. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rschoon <@t> email.unc.edu Wed Mar 2 09:50:22 2005 From: rschoon <@t> email.unc.edu (rschoon) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] The Histology Career was monkeys In-Reply-To: <590F08A18A72AF46917BF721E6476B5C226BAF@EXCHANGE2.ad.uams.edu> References: <590F08A18A72AF46917BF721E6476B5C226BAF@EXCHANGE2.ad.uams.edu> Message-ID: <4225E0BE.805@email.unc.edu> Here I go on a short rant..... So read no further if easly offended. It is true that most anyone with normal co-ordination can be taught to "turn a crank" and get sections from the microtome as well as load an automatic stainer /processoer, push various buttons and put labels on slides. Don't know about monkeys but I have trained MD's ( humor is intended here but I know, hate mail is on the way) to do manual IHC with acceptable results. The old "take them out of housekeeping and let the pathologist train them to section" mentality. The point is that most "techniques" can be taught but what happens when things go wrong? The stuff nightmares are made of!!! The following examples were drawn from a short stint as a very partime positon I held in a large hospital path lab where I was the only HTL and one of two HT's out of eight "technicians" (the supervisor was also not registered). 1) The person doing Special Stains (automated) asked me why the acid alcohol was not decolorizing his PAS slides. I asked why he wanted to do that and he replied that the hematoxylin on the stainer had run out and he wanted to restian the slides and that the "pink" wouldn't come out. I explained acid/ base dye's but stoped short of trying to explain the Shiff's reaction when I realized I wasn't getting through. Finally just told him to put the slides in the hematoxylin of the H&E stainer and that all woud be well. 2) The person doing the microscopic QA on the H&E's before the slides were delivered to the pathologist could certainly reccognize folds and knife lines in the sections but came to me one evening and asked me to look at some slides that didn't have any "blue dots" on them. Briefly explained cells and nuclei , suggested we check the automatic H&E stainer and discovered that someone had forgotten to put the "H" in the stainer....... She had been promoted to "technician" from accessioning clerk several months before...... I could go on as there were numerous othere incidents, some with legal ramifications, that happened on a daily basis that simply would NOT have occurred had the laboratory benn staffed with certified HT's. One must be able to understand what one is doing in order to fix what went wrong when it does (check Murphy's Laws of Histology, it will). With the increase complexity of the procedures and tests that we now do, IHC, IA etc. education is a must. Robert Schoonhoven, HT, HTL (ASCP) From Rcartun <@t> harthosp.org Wed Mar 2 10:40:43 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Immunostaining on bone marrow smears Message-ID: I've noticed a loss of immunoreactivity for certain proteins in air-dried smears after 2-3 days. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "M.Vici" 03/02/05 10:20AM >>> Hi everybody! I've just joined the histonet. My question: I need to immunostain old-air dried- bone marrow smears. I'm using an anti-nucleophosmin monoclonal antibody (nucleolus). Which fixation and peroxidase suppression protocols would you suggest? Thanks! Manuela _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Wed Mar 2 10:42:56 2005 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] cassette writer Message-ID: Could I please receive information on a good cassette writer? I am looking for one that etches the cassette rather then using inks or a label. Also could you list pros and cons on the machine if you have any? Thanks! Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 From gcallis <@t> montana.edu Wed Mar 2 10:46:28 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:42 2005 Subject: NSH and CEU's other than taking workshops Re: [Histonet] ASCP In-Reply-To: <49547.208.186.240.165.1109722782.squirrel@email.relia.net> References: <49547.208.186.240.165.1109722782.squirrel@email.relia.net> Message-ID: <6.0.0.22.1.20050302091834.01b64e70@gemini.msu.montana.edu> Go to NSH website (www.NSH.org and check out education opportunities. State societies and NSH regions closer to people provide CEU workshops. NSH will have an online Continuing Education course starting in May, 2005. and I foresee this as a very popular route to obtain/update CEU's. Also, teleconferences in your part of the US - I would think a hospital could let you participate in one even if you were not working f - you would have to pay the fees of course. There are also teleconferences out of Texas. Not sure about continuing education loans, but NSH may have information on application for these. Take a look at list of schools of histotechnology - maybe they have ways to update CEU's for those working or taking leave for a time but close to where you live. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From GDawson <@t> dynacaremilwaukee.com Wed Mar 2 10:57:02 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] ASCP Message-ID: I came in at the middle of this thread so excuse me if someone stated this before. To maintain the QIHC qualification, you not only have to dig up documentation on a minimum of 30 CEU's related to immunohistochemistry every 5 years, but you have to pay $50 to maintain it. Although I believe it is vital that IHC people stay up to snuff on continuing education, I think it is obnoxious to have to pay for a qualification that you've had to pay to obtain already. The $50 every 5 years is not an exhorbitant amount but it is more the principle. It seems like a fee just for the sake of a fee and it does nothing for the person that has to pay it. I paid to be a member of NSH for a number of years only because the mailings I received were very aggressive and led me to believe that my certification would lapse if I didn't pay. I haven't renewed since I learned for certain that this was not the case. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From kbroomal <@t> NEMOURS.ORG Wed Mar 2 11:07:43 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] ASCP Certificate Maintenance Program Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A7949@wlmmsx01.nemours.org> This link may be helpful to some with questions regarding CEU's. http://www.ascp.org/bor/cmp/index.asp There's also a FAQ page. http://www.ascp.org/bor/cmp/cmp_faq.asp Kristen Broomall, HT (ASCP) From David.Edmondson <@t> christie-tr.nwest.nhs.uk Wed Mar 2 11:48:49 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] removal of carbohydrates/glycosylation Message-ID: I wonder that oxidation will do this but that there will be consequences for the antigens that you are working with. Years ago, I remember using Periodic acid and then Borohydride as a peroxidase block but that PD726 samples from Oxford did not work at all. The LCA is obviously a run of the mill antibody now and carbohydrate moities a significant part thereof. Any suggestions/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sharon Cooperman Sent: 02 March 2005 15:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] removal of carbohydrates/glycosylation Hi, Does anyone know of a method to remove carbohydrate modifications/glycosylation from proteins in tissue sections on slides prior to immunohistochemistry? (I know it's a weird question.) Any suggestions, references, etc. would be greatly appreciated. Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-8417 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Wed Mar 2 12:06:51 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Flow Cytometer Message-ID: Hey all, I'm in the market to purchase a flow cytometer. Any suggestions? This is a new area for me and I'm not that well versed. I appreciate your, in advance. Joe Nocito Histology Manager Pathology Reference Lab San Antonio, TX From JNocito <@t> Pathreflab.com Wed Mar 2 12:19:26 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] ASCP In-Reply-To: Message-ID: I'll probably get flamed here, but it's been a while since I've been flamed. I'm having real issues with ASCP these days. Last year, two of my techs, who I trained took the registry. They both got their tissue from the same autopsy, processed the tissue in the same processor and stained the slides on the same stainer. One passed, the other did not. Now, my medical director and I looked at every slide these techs made. The techs were getting upset with me because I was kicking back many of their slides for folds, wrinkles, uneven staining, etc. I called ASCP to find out how the slides can be re-screened. I told them that I was the supervisor and questioned whomever graded the slides. I was told that it would cost $100 to review the slides again. I then proceeded to tell this person what if the grader made a mistake. Too bad, it would cost $100. Get this, the American Association of Pathologists Assistants (AAPA) have aligned with ASCP. Before this alliance, to take the exam by AAPA was $150, now, I understand that the same exam given by ASCP is going to cost me $450. Is ASCP in it for the money? You bet, but what can you do when they are the only game in town? Let the flaming begin. Joe Nocito Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawson, Glen Sent: Wednesday, March 02, 2005 10:57 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP I came in at the middle of this thread so excuse me if someone stated this before. To maintain the QIHC qualification, you not only have to dig up documentation on a minimum of 30 CEU's related to immunohistochemistry every 5 years, but you have to pay $50 to maintain it. Although I believe it is vital that IHC people stay up to snuff on continuing education, I think it is obnoxious to have to pay for a qualification that you've had to pay to obtain already. The $50 every 5 years is not an exhorbitant amount but it is more the principle. It seems like a fee just for the sake of a fee and it does nothing for the person that has to pay it. I paid to be a member of NSH for a number of years only because the mailings I received were very aggressive and led me to believe that my certification would lapse if I didn't pay. I haven't renewed since I learned for certain that this was not the case. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Wed Mar 2 12:42:22 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 From thoward <@t> unm.edu Wed Mar 2 13:00:04 2005 From: thoward <@t> unm.edu (Tamara Howard) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] paraffin embedding center suggestions? Message-ID: We are hoping to be able to get a new paraffin embedding center and I'd be interested in any comments, positive or negative, about any of the new models on the market. If you'd prefer to reply directly via e-mail, instead of via the list, please do so - all comments will be kept in confidence. I'm interested in service experience as well as ease of use - we are a multi-user research group. Thanks! Tamara |--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward@unm.edu |--------------------------------------------------| From lucyb <@t> biocare.net Wed Mar 2 13:01:36 2005 From: lucyb <@t> biocare.net (Lucy Brooks) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment References: Message-ID: <001301c51f5a$447a7180$0201a8c0@LUCYSALES> David Davis from the University of Colorado, has a business called Advanced Innovations, he has designed and created a wonderful Microtome Aligner! Biocare Medical uses it and it is a wonderful product. Contact David Davis at djdavis408@cs.com business phone = 303-949-7506. If you need any additional information I will be happy to help you! Hope this helps! Lucy Brooks Biocare Medical ----- Original Message ----- From: "Bartlett, Jeanine" To: Sent: Wednesday, March 02, 2005 10:42 AM Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joeamateur <@t> hotmail.com Wed Mar 2 13:23:23 2005 From: joeamateur <@t> hotmail.com (Jack England) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Hematoxylin[Scanned] In-Reply-To: <1030B679AD69D6119C3F00080210DD9D05A3F032@bhrv-nt-11.bhrv.nwest.nhs.uk> Message-ID: So what's wrong with being an alchemist? Those folks did a lot for the science of their day and whenever I spend a little time on StainsFile I often feel that we histo people carry on their legacy. (Sirius red F3B! Crystal Violet! Light Green SF Yellowish! Eye of newt! etc...) This is one of the things I love about this field, that there is so much art left to the science. After all, many of the most interesting discoveries and innovations in "modern" science of any era were and are made by curious folks wandering off in the weeds somewhere. Per the hematoxylin thread, we're quite fond of Richard-Allen Scientific's Gill 2 hematoxylin, as well, though we've used a Weigert's formula from an American MasterTech kit with great results (we're a research lab, not a clinical one, so we run a low volume and kits are economical for us). To date we've only bought solutions, but at the moment, I'm ripening a couple of bottles of hematoxylin right now, just to learn how it's done. Good luck! --Aloha, Jack England Tissue Genesis, Inc. http://www.tissuegenesis.com >From: Kemlo Rogerson >To: "'Bauer, Karen'" >CC: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Hematoxylin[Scanned] >Date: Wed, 2 Mar 2005 07:49:17 -0000 > >Good girl.... > >Gone are the days when I had 5 litre flasks of Harris's haematoxylin >ripening in the Stoke-on-Trent sunshine; I felt like an alchemist. Then it >was plagiarised 'Gills' haematoxylin ripening, then some infernal lead >haematoxylin concoction that turned everything it touched black; I >strangely >put on weight too. > >The ripening of haematoxylin to haematin then to oxyhaematin was a delight >to witness, the latter meant you have gone too far. The ability to carry >out >an H&E progressively without having to remove the cytoplasmic >'overstaining' >a delight. > >I'm one for keeping 'home made' haematoxylin together with retaining the >'a' >in haematin, but on both points alas I feel I shall fail. > _________________________________________________________________ Don’t just search. Find. Check out the new MSN Search! http://search.msn.click-url.com/go/onm00200636ave/direct/01/ From maxim_71 <@t> mail.ru Wed Mar 2 13:44:48 2005 From: maxim_71 <@t> mail.ru (Maxim) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Silver stains Message-ID: <584883796.20050302224448@mail.ru> Dear histonettters! Can anyone send me copy of the article Garvey W. Silver stains. Journal of Histotechnol. 1996;19(3):203-209, if it possible in any type (better in e-mail). In Russian library's not available this journal nor for what year. Thank you. Maxim Peshkov, HTL. Department of biopsy and cytological researches. Pathological and anatomical bureau. Russia, Taganrog. Post address: Maxim Peshkov, Nijnjaja Linija, 93 Taganrog, Russia. 347942. mailto:maxim_71@mail.ru From gentras <@t> vetmed.auburn.edu Wed Mar 2 14:04:57 2005 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:24:42 2005 Subject: Fwd: [Histonet] (no subject) Message-ID: <6.0.1.1.0.20050302140434.01b26c80@mailhost.vetmed.auburn.edu> I second that motion!!! > I think CAP needs >to implement a set of regulations for histo techs. We have been too long in >the gray area unlike most fields, who have specific regulations to what they >can and can not do. From gentras <@t> vetmed.auburn.edu Wed Mar 2 14:07:11 2005 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:24:42 2005 Subject: Fwd: [Histonet] CAP question - liquid nitrogen Message-ID: <6.0.1.1.0.20050302140627.01b26dc8@mailhost.vetmed.auburn.edu> Please post answers to this question to the histonet??? > am asking this question for another lab: Does anyone out there have a > procedure on the handling of liquid nitrogen that they would be willing > to share? This is in reference to a new question on the CAP > checklist. It's on the Lab General checklist, GEN.70525 - "Are adequate > policies, procedures, and practices in place for the use of liquid nitrogen?" > >I would appreciate any help. Thanks! > >Laurie Colbert >Huntington Memorial Hospital >Pasadena, CA From stevemachinuk <@t> yahoo.co.uk Wed Mar 2 14:07:54 2005 From: stevemachinuk <@t> yahoo.co.uk (Steve Machin UK) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment In-Reply-To: <001301c51f5a$447a7180$0201a8c0@LUCYSALES> Message-ID: <20050302200754.39217.qmail@web25104.mail.ukl.yahoo.com> A few year ago I put the method I use to align blocks on the histonet website as a web page. It does not require any extra eqiupment and will align blocks anywhere in the world. If we all used this method all our microtome cassettes holders would be aligned with eachothers. http://www.histosearch.com/homepages/stevemachin.html --- Lucy Brooks wrote: > David Davis from the University of Colorado, has a business called > Advanced > Innovations, he has designed and created a wonderful Microtome > Aligner! > Biocare Medical uses it and it is a wonderful product. Contact > David Davis > at djdavis408@cs.com business phone = 303-949-7506. > > If you need any additional information I will be happy to help you! > Hope > this helps! > > Lucy Brooks > Biocare Medical > > ----- Original Message ----- > From: "Bartlett, Jeanine" > To: > Sent: Wednesday, March 02, 2005 10:42 AM > Subject: [Histonet] block alignment > > > Hi: > > Does anyone out there have any experience with block alignment > products > such as the Microtome Aligner from Newcomer or the Histo > Collimator? We > receive a huge number of outside blocks and I adjust my block > holder > accordingly, but others in the group say it's easier to re-embed > all > outside blocks (Yikes!) and then have every microtome aligned the > same. > I was just wondering if these aligners work and if so perhaps it > could > be an alternative for those individuals that normally would rather > re-embed than realign. > > Thanks! > Jeanine Bartlett, HT(ASCP) > Centers for Disease Control and Prevention > Infectious Disease Pathology Activity > 1600 Clifton Road, MS/G-32 > Atlanta, GA 30333 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Send instant messages to your online friends http://uk.messenger.yahoo.com From BennettW <@t> pac.dfo-mpo.gc.ca Wed Mar 2 15:48:33 2005 From: BennettW <@t> pac.dfo-mpo.gc.ca (BennettW@pac.dfo-mpo.gc.ca) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Razor Clams Message-ID: <7CBBD627E4E688499349A5D11D078316020DBBBC@msgpacpbs.rhq.pac.dfo-mpo.gc.ca> Hello Histonetters, I have a researcher that is ageing (aging) Razor Clams and would like to better differentiate the conchiolin, a keratin like substance, versus the calcium carbonate of the shell. The growth rings consist of alternating bands of calcium carbonate and conchiolin ( as far as I can find out conchiolin is keratin "like"). A smooth facet of the shell is produced after a diamond saw is used to slice it in two. The growth bands are in the smooth facet but difficult to see. The bands in older animals are very close together and very difficult to see. Does anyone have any suggestions re: stains or procedures that might help better differentiate the bands? Thanks in advance for any help. Cheers Bill Bennett Fisheries and Oceans Canada Pacific Biological Station Nanaimo, B.C. Canada From Barry.R.Rittman <@t> uth.tmc.edu Wed Mar 2 16:01:30 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Razor Clams Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0013F20A0@UTHEVS3.mail.uthouston.edu> Bill Talking through the back of my head - I know nothing about clams - I suspect that the growth rings may differ significantly in their absorption of many dyes. You may want to try to expose the cut surface of the shell to a dye such as dilute methylene blue to see if this is true. Could also use Procion dyes (chloro-s-triazines) to stain the protein component. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BennettW@pac.dfo-mpo.gc.ca Sent: Wednesday, March 02, 2005 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Razor Clams Hello Histonetters, I have a researcher that is ageing (aging) Razor Clams and would like to better differentiate the conchiolin, a keratin like substance, versus the calcium carbonate of the shell. The growth rings consist of alternating bands of calcium carbonate and conchiolin ( as far as I can find out conchiolin is keratin "like"). A smooth facet of the shell is produced after a diamond saw is used to slice it in two. The growth bands are in the smooth facet but difficult to see. The bands in older animals are very close together and very difficult to see. Does anyone have any suggestions re: stains or procedures that might help better differentiate the bands? Thanks in advance for any help. Cheers Bill Bennett Fisheries and Oceans Canada Pacific Biological Station Nanaimo, B.C. Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peoshel <@t> wisc.edu Wed Mar 2 16:05:06 2005 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Razor Clams In-Reply-To: <7CBBD627E4E688499349A5D11D078316020DBBBC@msgpacpbs.rhq.pac.dfo-mpo.gc.ca> References: <7CBBD627E4E688499349A5D11D078316020DBBBC@msgpacpbs.rhq.pac.dfo-mpo.gc.ca> Message-ID: Bill, Look for publications by Kent Gilkinson (I think I spelled that right), probably Gilkinson & Steele, and Gilkinson, K. G., J. A. Hutchings, P. E. Oshel, & R. L. Haedrich. 1986. Shell microstructure and observations on internal banding patterns in the bivalves Yoldia thraciaeformis Storer 1838 and Nuculana pernula Muller 1779 (Nuculanidae) from a deep-sea environment. Veliger 29(1):70-77. I give this last ref only because I have the reference handy. The better references will be Gilkinson or Gilkinson & Steele. Kent did an aging study in blue mussels from the Labrador Strait. The basic method is to make two radial saw cuts from the prochonch (? correct? I forget) to the mantle edge, polish one side, glue it to a rock-section slide, then finely polish the other side, polishing until the shell section is thin enough to allow light through. The growth bands are then easily visible, even tightly packed ones like those of very Old mussels from cold waters. Phil >Hello Histonetters, > >I have a researcher that is ageing (aging) Razor Clams and would like to >better differentiate the conchiolin, a keratin like substance, versus the >calcium carbonate of the shell. The growth rings consist of alternating >bands of calcium carbonate and conchiolin ( as far as I can find out >conchiolin is keratin "like"). A smooth facet of the shell is produced >after a diamond saw is used to slice it in two. The growth bands are in >the smooth facet but difficult to see. >The bands in older animals are very close together and very difficult to >see. Does anyone have any suggestions re: stains or procedures that might >help better differentiate the bands? >Thanks in advance for any help. > >Cheers >Bill Bennett >Fisheries and Oceans Canada >Pacific Biological Station >Nanaimo, B.C. >Canada -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From cmconway <@t> usgs.gov Wed Mar 2 16:37:50 2005 From: cmconway <@t> usgs.gov (Carla M Conway) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] permeabilization needed in IHC of viral protein? Message-ID: Hello, I am interested in using IHC to detect nucleocapsid (N) protein within rhabdovirus. I have had success in detecting the glycoprotein on the envelope surface, however the N protein is located within the virion. Sections (FFPE) will be enzymatically digested with 0.05% protease XIV in TBS according to our standard protocol. Would a permeabilization agent increase access to the interior proteins? Any suggestions/comments will be greatly appreciated. Thank you, Carla Conway Western Fisheries Research Center 6505 NE 65th St Seattle, WA 98115 ph: 206-526-6282 ext. 242 fax: 206-526-6654 From Linresearch <@t> aol.com Wed Mar 2 17:14:40 2005 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Negative Serum for Anti-chicken Ab Message-ID: <128.57a6e25d.2f57a2e0@aol.com> Hi, Can anyone suggest a negative serum to use with a goat x chicken antibody? I need to confirm that the localization that I am seeing is accurate. I know that: Neg Rat Ab & a Neg Rabbit Ab that can be purchased to confirm rat and rabbit abs. Lin From histology.bc <@t> shaw.ca Wed Mar 2 18:13:54 2005 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Hematoxylin and commercial reagents Message-ID: <422656C2.60602@shaw.ca> Good for you, Karen. Like Kemlo, I came from the school of home-made hematoxylin. The lab windows were ringed with flasks of hematoxylin at varying stages of maturity. Ehrlich's, Harris's, and Carazzi's were our favourite brews, carefully chosen for specific purposes. Home brewing requires some planning to anticipate future needs. Ehrlich's requires 6-12 months to reach fully maturity. A well ripened batch of Ehrlich's has an aroma that has to be experienced to be believed. The aroma is like a fine, well-aged port (although the taste is nothing close !!) Cost-wise, home made hematoxylins make a lot of sense. They are way cheaper than any commercially-prepared equivalent. They are not dificult to prepare, anybody who can read can make hematoxylin solutions. One of my pet peeves is the increasing reliance on commercially-prepared reagents for histotechnology. The manufacturers will sell any reagent/solution that we are prepared to buy ... for a price that is far, far beyond what it is worth ... and then we complain that we need bigger budgets. I shake my head when I read Histonet messages from subscribers who are looking for prepared solutions of neutral red, alcian blue, eosin, etc. These must be the same people who buy canned, mashed potatoes, pre-cooked rice, or any other "have dinner ready in 2 minutes" food. I am not suggesting that you try to brew up your own IHC reagents. Some things should be left to the commercial labs. But for hematoxylins, eosin, Schiff reagent, routine stains, reagents for Perls' Prussian blue, B-5 fixative, etc. ... save your money, make your own. Keep up the good work. Don't forget to let your employer know that you are saving them a fortune by preparing your own solutions. Some employers reward members of their staff who come up with cost-saving ideas. Just my thoughts for what they are worth, like Kemlo I am getting old and opinionated, Paul Bradbury, Kamloops, BC From Gervaip <@t> aol.com Wed Mar 2 19:42:09 2005 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] ASCP Message-ID: Joe, I agree with you, the ASCP is not very nice to deal with. I had a past experience where one of my employees had the IHC and she knew she had to have so many CEUs in order to renew. A few months before her renewal she finds out that PACE was not going to be accepted! More then half her credits were PACE. When I called to inquire as to why they no longer would accept PACE, they had attitude! And guess what, now the ASCP is going to accept PACE credits! I think the ASCP was trying to get everyone to get credits through them... to make more money. Like they don't make enough already. I am not happy with them, but like you said... they are the only game in town! Pearl From ninian_h <@t> yahoo.com Wed Mar 2 19:46:03 2005 From: ninian_h <@t> yahoo.com (ninian H) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] TH and golgi-cox? Message-ID: <20050303014603.93602.qmail@web50104.mail.yahoo.com> Hi all, Does anyone know about combining golgi-cox with tyrosine hydroxylase staining? the main purpose of mine is to distinguish different layers of cortex and TH positive fibers. I'll appreciate any information that might help me to see both pyramydal cells and TH containing fibers in cortical areas. Also co-staining with TH and neutral red didn't work out, and TH -stained neurons didn't stain with red color , any idea or suggestion? Thank you in advance, Ninian, ph.D student histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwesten.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: removal of carbohydrates/glycosylation (Edmondson David (RBV) NHS Christie Tr) ---------------------------------------------------------------------- Message: 1 Date: Wed, 2 Mar 2005 17:48:49 -0000 From: "Edmondson David \(RBV\) NHS Christie Tr" Subject: RE: [Histonet] removal of carbohydrates/glycosylation To: "Sharon Cooperman" Cc: "Histonet \(E-mail 2\)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I wonder that oxidation will do this but that there will be consequences for the antigens that you are working with. Years ago, I remember using Periodic acid and then Borohydride as a peroxidase block but that PD726 samples from Oxford did not work at all. The LCA is obviously a run of the mill antibody now and carbohydrate moities a significant part thereof. Any suggestions/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sharon Cooperman Sent: 02 March 2005 15:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] removal of carbohydrates/glycosylation Hi, Does anyone know of a method to remove carbohydrate modifications/glycosylation from proteins in tissue sections on slides prior to immunohistochemistry? (I know it's a weird question.) Any suggestions, references, etc. would be greatly appreciated. Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-8417 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 5 *************************************** --------------------------------- Celebrate Yahoo!'s 10th Birthday! Yahoo! Netrospective: 100 Moments of the Web From leahcox27 <@t> yahoo.com Wed Mar 2 20:24:23 2005 From: leahcox27 <@t> yahoo.com (Leah Cox) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Deeper vs Levels In-Reply-To: <4224FFB3.5000003@pathology.washington.edu> Message-ID: <20050303022423.92187.qmail@web50203.mail.yahoo.com> I do recuts for two different pathologists. One of them uses the term "deeper" and the other uses "levels" and they both mean the same thing (in our lab anyway)! I always cut 3 levels!!! GOOD LUCK! Victor Tobias wrote:We have this on going issue of the use of the terms such as deeper vs levels. For this example let's use 3 levels vs 3 deeper sections. There is no question regarding the understanding of 3 levels, but what about 3 deeper sections? My immediate boss says 3 deeper sections are essentially 3 recuts at one deeper level. In talking with some of the techs, they would treat 3 deeper sections the same as 3 levels. We want to clean-up our database and eliminate any confusion. Pathologist thinks they are ordering this and get that. Any comments? Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Celebrate Yahoo!'s 10th Birthday! Yahoo! Netrospective: 100 Moments of the Web From mucram11 <@t> comcast.net Wed Mar 2 20:31:49 2005 From: mucram11 <@t> comcast.net (pam marcum) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Job Change Message-ID: <42267715.000003.03524@YOUR-4DI1S53IME> Hi to All My Friends and Fellow HistoNetters, I will be changing jobs starting Monday. I have enjoyed working at Polysciences for the last five years and have now decided to leave and go back to a research laboratory for plastics full time. I am very excited and happy to be moving to the University of Pennsylvania, School of Veterinary Medicine doing plastics and special procedures for the school and some contract work. I hope to see many of you at one of the meetings I will attend this year or at NSH. Anyone who wants to contact can reach me through my home e-mail for now. Pam Marcum Polysciences will be looking for a replacement in the Histology/Microscopy area so anyone interested can contact either Jennifer Tenfelde in Human Resources or Debra Sesholtz at sesholtz@polysciences.com for information and requirements for the position. From sbindu20002000 <@t> yahoo.co.in Wed Mar 2 22:04:21 2005 From: sbindu20002000 <@t> yahoo.co.in (s bindu) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Haematoxylin Message-ID: <20050303040421.37577.qmail@web8506.mail.in.yahoo.com> Hi, It is very happy to hear this. In our lab too we are preparing our own hamatoxylin and eosin and getting good results. Regards Bindu.S SCTIMST,TVM. sbindu20002000@yahoo.co.in Yahoo! India Matrimony: Find your life partneronline. From Kemlo.Rogerson <@t> elht.nhs.uk Thu Mar 3 01:54:59 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Hematoxylin[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F049@bhrv-nt-11.bhrv.nwest.nhs.uk> Send me a bit? We don't get it anymore in these parts; has to have a kit mark! -----Original Message----- From: Jack England [mailto:joeamateur@hotmail.com] Sent: 02 March 2005 19:23 To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hematoxylin[Scanned] So what's wrong with being an alchemist? Those folks did a lot for the science of their day and whenever I spend a little time on StainsFile I often feel that we histo people carry on their legacy. (Sirius red F3B! Crystal Violet! Light Green SF Yellowish! Eye of newt! etc...) This is one of the things I love about this field, that there is so much art left to the science. After all, many of the most interesting discoveries and innovations in "modern" science of any era were and are made by curious folks wandering off in the weeds somewhere. Per the hematoxylin thread, we're quite fond of Richard-Allen Scientific's Gill 2 hematoxylin, as well, though we've used a Weigert's formula from an American MasterTech kit with great results (we're a research lab, not a clinical one, so we run a low volume and kits are economical for us). To date we've only bought solutions, but at the moment, I'm ripening a couple of bottles of hematoxylin right now, just to learn how it's done. Good luck! --Aloha, Jack England Tissue Genesis, Inc. http://www.tissuegenesis.com >From: Kemlo Rogerson >To: "'Bauer, Karen'" >CC: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Hematoxylin[Scanned] >Date: Wed, 2 Mar 2005 07:49:17 -0000 > >Good girl.... > >Gone are the days when I had 5 litre flasks of Harris's haematoxylin >ripening in the Stoke-on-Trent sunshine; I felt like an alchemist. Then it >was plagiarised 'Gills' haematoxylin ripening, then some infernal lead >haematoxylin concoction that turned everything it touched black; I >strangely >put on weight too. > >The ripening of haematoxylin to haematin then to oxyhaematin was a delight >to witness, the latter meant you have gone too far. The ability to carry >out >an H&E progressively without having to remove the cytoplasmic >'overstaining' >a delight. > >I'm one for keeping 'home made' haematoxylin together with retaining the >'a' >in haematin, but on both points alas I feel I shall fail. > _________________________________________________________________ Don't just search. Find. Check out the new MSN Search! http://search.msn.click-url.com/go/onm00200636ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Thu Mar 3 01:59:15 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Hematoxylin and commercial reagents[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F04A@bhrv-nt-11.bhrv.nwest.nhs.uk> Me opinionated???? Old I concede but hold on! Only jesting....................... -----Original Message----- From: Paul Bradbury [mailto:histology.bc@shaw.ca] Sent: 03 March 2005 00:14 To: HistoNet Server Subject: [Histonet] Hematoxylin and commercial reagents[Scanned] Good for you, Karen. Like Kemlo, I came from the school of home-made hematoxylin. The lab windows were ringed with flasks of hematoxylin at varying stages of maturity. Ehrlich's, Harris's, and Carazzi's were our favourite brews, carefully chosen for specific purposes. Home brewing requires some planning to anticipate future needs. Ehrlich's requires 6-12 months to reach fully maturity. A well ripened batch of Ehrlich's has an aroma that has to be experienced to be believed. The aroma is like a fine, well-aged port (although the taste is nothing close !!) Cost-wise, home made hematoxylins make a lot of sense. They are way cheaper than any commercially-prepared equivalent. They are not dificult to prepare, anybody who can read can make hematoxylin solutions. One of my pet peeves is the increasing reliance on commercially-prepared reagents for histotechnology. The manufacturers will sell any reagent/solution that we are prepared to buy ... for a price that is far, far beyond what it is worth ... and then we complain that we need bigger budgets. I shake my head when I read Histonet messages from subscribers who are looking for prepared solutions of neutral red, alcian blue, eosin, etc. These must be the same people who buy canned, mashed potatoes, pre-cooked rice, or any other "have dinner ready in 2 minutes" food. I am not suggesting that you try to brew up your own IHC reagents. Some things should be left to the commercial labs. But for hematoxylins, eosin, Schiff reagent, routine stains, reagents for Perls' Prussian blue, B-5 fixative, etc. ... save your money, make your own. Keep up the good work. Don't forget to let your employer know that you are saving them a fortune by preparing your own solutions. Some employers reward members of their staff who come up with cost-saving ideas. Just my thoughts for what they are worth, like Kemlo I am getting old and opinionated, Paul Bradbury, Kamloops, BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Krat18 <@t> aol.com Thu Mar 3 02:55:13 2005 From: Krat18 <@t> aol.com (Krat18@aol.com) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Hematoxylin Message-ID: <29.6e25fd19.2f582af1@aol.com> Our pathologists really like American Master Tech Hematoxylin. These companies will send you a free sample to try. _Click here: www.mastertechs.com: American Master*Tech Scientific, Inc. Main Page#products_ (http://www.americanhistology.com/#products) _Karen_Raterman@ssmhc.com_ (mailto:Karen_Raterman@ssmhc.com) From djamesnz <@t> orcon.net.nz Thu Mar 3 03:08:20 2005 From: djamesnz <@t> orcon.net.nz (Darren James) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] RE: disinfection of cryostat[Scanned] In-Reply-To: Message-ID: <200503030908.j23981P9028973@dbmail-mx4.orcon.co.nz> Similar thing happened to me with a punch bx of skin. Frozen section showed lots of granuloma and necrosis. The path asked for a Wade Fite and it turned out to be leprosy!! AFBs certainly wouldn't die in a cryostat. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 03 March 2005 02:57 To: Kemlo Rogerson Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Well, it was a hot day, and I had to get the Popsicles out, anyway . . . . . Scenario: You get a frozen on a lung sample to rule out tumor - you cut it - you know how frozens sometimes shatter and that stuff is all over - it's a granuloma - HEY! That might be Tuberculosis! You're hands have been in the cryostat (gloved) and what are the odds you wash your hands between cutting a section and staining it? Now you might have TB bacteria warmed up all over everything you touched outside the cryostat. It could happen. Kemlo Rogerson Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2005 07:28 AM To: "'Jackie M O'Connor'" cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] But why did you stick your head in a cryostat? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: 02 March 2005 12:48 To: Kemlo Rogerson Cc: 'Charles Scouten'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] No - but they don't die, either - so when you breathe them into your nice warm lungs - voila! They wake up and infect you. Jacqueline M. O'Connor HT(ASCP) Assistant Scientist Cancer Research Jackie.OConnor@abbott.com Kemlo Rogerson Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2005 01:50 AM To: "'Charles Scouten'" cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Do bugs grow at minus 20? -----Original Message----- From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: 01 March 2005 20:04 To: Hopkins, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Why disinfect the cryostat? Let the durn thing disinfect itself. IF you have this requirement, you may want a self disinfecting cryostat. See the link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=47510 2&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats &idsubcategory=182 Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hopkins, Karen Sent: Tuesday, March 01, 2005 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disinfection of cryostat Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.A.Harper <@t> pcola.med.navy.mil Thu Mar 3 05:54:15 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Need an opinion Message-ID: <807FE48C5A7CC940B973B58D32E7014318A76689@nhpens-exch1.pcola.med.navy.mil> I had written last week in regards to the sudden work change of me having to learn how to gross. I'm going to go ahead and learn but I need an opinion. I have 30 days to learn how to gross. Presently, the pathologist is training a cyto tech to gross. She got trained last week and this week is flying solo and she is very unsure of what she is doing and I have been telling her what she has to do. She already lost 2 cxbx on a case. I also observed this same type of training with my military co-worker. Trained one week, flying solo the next and that particular pathologist isn't always in his office. So she would have to de-glove and pick up the phone and call a pathologist. If you were in my situation, tell me in your opinion what 30 days of training would entail because maybe I'm expecting too much. I believe for those 30 days, the pathologist should be standing in the background, watching, listening and observing, no matter how fast you caught on to grossing and be there for any questions. I start training April 1st and would just like to know what the histo world would expect. Thanks in advance for any opinions. Heather A. Harper From Kemlo.Rogerson <@t> elht.nhs.uk Thu Mar 3 06:36:57 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Need an opinion[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F04C@bhrv-nt-11.bhrv.nwest.nhs.uk> You need at the very least a Standard Operational procedure for 'grossing'. This will have each specimen and how the Pathologist has trained you to 'cut it'. For example skin biopsies of a ?mole, would be cut TS with three slices across the mole. The ends retained 'just in case'. The same for anything else you dissect or 'put in'. That is each sample with have a procedure for you to follow, which you do with no alteration. I would have thought that if a Pathologist sat in for a few days whilst you carried out the 'cut up' then you would learn quickly; if I was the Pathologist then I would occasionally revisit to make sure. It really depends on your experience and training; the issues relating to tissue 'cross over' (on forceps, cut up board, etc) are maybe the most important, but other issues concerning under fixed tissue and your ability to know when to ask for help given an unusually finding need to be addressed. The latter is one of the most important; you can train people to do almost anything, the trick is to get them to recognise when they are 'stepping out' of that training (i.e. it's not a mole, but the Clinical data and gross macro suggests that a melanoma may be a likelihood, then you refer). Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Heather.A.Harper@pcola.med.navy.mil [mailto:Heather.A.Harper@pcola.med.navy.mil] Sent: 03 March 2005 11:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need an opinion[Scanned] I had written last week in regards to the sudden work change of me having to learn how to gross. I'm going to go ahead and learn but I need an opinion. I have 30 days to learn how to gross. Presently, the pathologist is training a cyto tech to gross. She got trained last week and this week is flying solo and she is very unsure of what she is doing and I have been telling her what she has to do. She already lost 2 cxbx on a case. I also observed this same type of training with my military co-worker. Trained one week, flying solo the next and that particular pathologist isn't always in his office. So she would have to de-glove and pick up the phone and call a pathologist. If you were in my situation, tell me in your opinion what 30 days of training would entail because maybe I'm expecting too much. I believe for those 30 days, the pathologist should be standing in the background, watching, listening and observing, no matter how fast you caught on to grossing and be there for any questions. I start training April 1st and would just like to know what the histo world would expect. Thanks in advance for any opinions. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fjones <@t> namsa.com Thu Mar 3 07:20:38 2005 From: fjones <@t> namsa.com (Fawn Jones) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Training Message-ID: <915E55B02E236E4D95258B181EEF6317075DC5@namsams01.namsa.int> I am having a new situation starting to occur, where our company wants to hire a high school student to help ship our slides and now they are telling us she can gross our tissues also. We work in a medical device testing company where we gross everything from muscles, to spines, to all organs from mice, rats, rabbits, dogs, swine, sheep and guinea pigs. These are not necessarily easy things to gross as we have to look for test articles and implant sites (which may not be visible macroscopically), and determine if there are any changes in the tissues. I'm not sure we are qualified enough ourselves to be doing these things let alone a high school senior. If anybody could send any information on the qualifications needed for this type of work so that we could show our managers, (which are not histotechs and have no histology experience), that hiring a high school senior to do grossing is not a good idea. We also will not have a pathologist on hand to help with grossing of these tissues or for training. There are no set standards for grossing as most of our special studies have different test articles and different things they are looking for, so no two of these studies are really ever the same. Thank you in advance Fawn Jones From GDawson <@t> dynacaremilwaukee.com Thu Mar 3 07:27:43 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu Mar 3 07:44:18 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D1DD@sjhaexc02.sjha.org> From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Julie.Sanders <@t> med.va.gov Thu Mar 3 07:46:23 2005 From: Julie.Sanders <@t> med.va.gov (Julie.Sanders@med.va.gov) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Basic ISH and IHC Message-ID: <457381D92B01BD44B21CF37CC02EBDFD02927596@vhacinexc5.v10.med.va.gov> Hello all, I am looking for any books/information regarding basic (very basic) theories and explanations for ISH and IHC. We do ISH in our lab and know the technique, but not the theory, so I'm trying to find information on these subjects. I've looked at Amazon.com, but most of the books seem way beyond "basic." What we are looking for is something that can give us at least "beginners" information. I spoke to our Ventana rep. and suggested that Ventana should put on regional workshops. (are you reading this Ventana?) With so many histology labs using this technology it would be helpful to have background. Its easy to slap some slides on a machine, put the dispensers on, click start and walk away....now we need to know the how and why we do this. Any information/suggestions would be greatly appreciated! Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VA Medical Center 3200 Vine St. ML113 Cincinnati, Ohio 45220 From JQB7 <@t> CDC.GOV Thu Mar 3 07:48:54 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From ktournear <@t> cox.net Thu Mar 3 07:59:31 2005 From: ktournear <@t> cox.net (ktournear@cox.net) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] CAP survey slids Message-ID: <20050303135931.HMQC19936.fed1rmmtao09.cox.net@smtp.west.cox.net> Hi out there, I have the new CAP survey IHC slides for 5 pathologists. My problem is, is that I only have 4 cases/10 slides each case and each pathologist is supposed to do this now. Can anyone tell me how this is done? I don't think I have enough slides for all of them. Thanks!! Kim Tournear, HT (ASCP) QIHC Specialists in Dermatology PLLC Tucson, AZ From JWEEMS <@t> sjha.org Thu Mar 3 08:10:41 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D1DF@sjhaexc02.sjha.org> It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From dsnider <@t> shrinenet.org Thu Mar 3 08:19:23 2005 From: dsnider <@t> shrinenet.org (Snider, Deanna) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Shandon Hypercenter XP Message-ID: Shriners Hospital for Children Research Dept. Cincinnatti, Oh 513-872-6388 Hello again everyone in Histoland! Does anyone use this processor? I had not used it prior to accepting this position. What seems to be the problem is that it seems to have a lot of carry over from station to station. I have read the manual, did all the maintenance it suggests, and I diligently keep up the daily routine maintenance. I process very small samples of clinically engineered skin. The largest workload I have ever had here was 34 blocks. Usually it is more like 8- 10 cassettes. I just changed all the reagents yesterday, including both paraffins. I had a run with 4 cassettes overnight and this morning the first paraffin station has a very strong xylene smell. Has anyone else found this to be a problem with this particular piece of equipment? I don't recall having this much of a problem with this in the VIP's or MVPs which I have worked with for the last 15 years. I read through the archives to see if anyone had mentioned this and found nothing, so your comments will be greatly appreciated. Thanks Deanna Snider HT CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From JQB7 <@t> CDC.GOV Thu Mar 3 08:14:47 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From kelly.mcqueeney <@t> bms.com Thu Mar 3 08:18:09 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Basic ISH and IHC In-Reply-To: <457381D92B01BD44B21CF37CC02EBDFD02927596@vhacinexc5.v10.med.va.gov> References: <457381D92B01BD44B21CF37CC02EBDFD02927596@vhacinexc5.v10.med.va.gov> Message-ID: <42271CA1.2020001@bms.com> Try the Roche website, it's pretty informative http://us.diagnostics.roche.com/ click on *research* then *printed materials* then *non-radioactive In Situ Hybridization Application Manual. *They give a nice description of the experiment, reagents, and WHY you use them. * For IHC, try *Current Protocols in Immunology. * http://www.does.org/masterli/cpi.html * Julie.Sanders@med.va.gov wrote: >Hello all, >I am looking for any books/information regarding basic (very basic) theories >and explanations for ISH and IHC. We do ISH in our lab and know the >technique, but not the theory, so I'm trying to find information on these >subjects. I've looked at Amazon.com, but most of the books seem way beyond >"basic." What we are looking for is something that can give us at least >"beginners" information. I spoke to our Ventana rep. and suggested that >Ventana should put on regional workshops. (are you reading this Ventana?) >With so many histology labs using this technology it would be helpful to >have background. Its easy to slap some slides on a machine, put the >dispensers on, click start and walk away....now we need to know the how and >why we do this. Any information/suggestions would be greatly appreciated! > >Julie Sanders, BA, HT(ASCP) >Supervisor, Anatomic Pathology >VA Medical Center >3200 Vine St. ML113 >Cincinnati, Ohio 45220 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mcauliff <@t> umdnj.edu Thu Mar 3 11:43:29 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] TH and golgi-cox? In-Reply-To: <20050303014603.93602.qmail@web50104.mail.yahoo.com> References: <20050303014603.93602.qmail@web50104.mail.yahoo.com> Message-ID: <42274CC1.7090405@umdnj.edu> Hi Ninian: As for Golgi-Cox, John Kiernan is the man to ask. As for a nuclear stain to go with TH, I have used Toluidine Blue, Thionin, Cresyl Violet and Celestine Blue B with excellent results. Assuming you are demonstrating TH with DAB, get some of Vector's "Intense", it turns the DAB a nice golden color which goes very well with any blue nuclear stain. If you are intensifying the DAB with nickel+cobalt a red nuclear stain would look nice. I don't know why neutral red did not work for you, I suggest a fresh batch. Or try "Scarba Red", a neutral red variant. See Slidders et al., J. Pathol. Bacterol. 75:476-478, 1958. Geoff ninian H wrote: >Hi all, >Does anyone know about combining golgi-cox with tyrosine hydroxylase staining? the main purpose of mine is to distinguish different layers of cortex and TH positive fibers. I'll appreciate any information that might help me to see both pyramydal cells and TH containing fibers in cortical areas. >Also co-staining with TH and neutral red didn't work out, and TH -stained neurons didn't stain with red color , any idea or suggestion? >Thank you in advance, >Ninian, ph.D student > > >histonet-request@lists.utsouthwestern.edu wrote: >Send Histonet mailing list submissions to >histonet@lists.utsouthwesten.edu > >To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to >histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at >histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > >1. RE: removal of carbohydrates/glycosylation >(Edmondson David (RBV) NHS Christie Tr) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Wed, 2 Mar 2005 17:48:49 -0000 >From: "Edmondson David \(RBV\) NHS Christie Tr" > >Subject: RE: [Histonet] removal of carbohydrates/glycosylation >To: "Sharon Cooperman" >Cc: "Histonet \(E-mail 2\)" >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > > >I wonder that oxidation will do this but that there will be consequences for the antigens that you are working with. Years ago, I remember using Periodic acid and then Borohydride as a peroxidase block but that PD726 samples from Oxford did not work at all. The LCA is obviously a run of the mill antibody now and carbohydrate moities a significant part thereof. >Any suggestions/ > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sharon >Cooperman >Sent: 02 March 2005 15:02 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] removal of carbohydrates/glycosylation > > >Hi, > >Does anyone know of a method to remove carbohydrate >modifications/glycosylation from proteins in tissue sections on >slides prior to immunohistochemistry? (I know it's a weird >question.) Any suggestions, references, etc. would be greatly >appreciated. > >Thanks, >Sharon > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From JWEEMS <@t> sjha.org Thu Mar 3 08:39:44 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D1E0@sjhaexc02.sjha.org> Unless it's changed, it doesn't fit Leicas. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 9:14 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From JQB7 <@t> CDC.GOV Thu Mar 3 08:43:40 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: The Histo Collimator doesn't, but the one from Newcomer does. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:40 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Unless it's changed, it doesn't fit Leicas. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 9:14 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Charles.Embrey <@t> carle.com Thu Mar 3 08:51:08 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Need an opinion Message-ID: Just for a comparison..... Pathologists' Assistants study two years to complete their degree to be able to gross. One week hardly seems adequate. Your Doc's must be glad that it is near to impossible to sue military doctors for malpractice. Poor patients. Charles Embrey -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Thursday, March 03, 2005 5:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need an opinion I had written last week in regards to the sudden work change of me having to learn how to gross. I'm going to go ahead and learn but I need an opinion. I have 30 days to learn how to gross. Presently, the pathologist is training a cyto tech to gross. She got trained last week and this week is flying solo and she is very unsure of what she is doing and I have been telling her what she has to do. She already lost 2 cxbx on a case. I also observed this same type of training with my military co-worker. Trained one week, flying solo the next and that particular pathologist isn't always in his office. So she would have to de-glove and pick up the phone and call a pathologist. If you were in my situation, tell me in your opinion what 30 days of training would entail because maybe I'm expecting too much. I believe for those 30 days, the pathologist should be standing in the background, watching, listening and observing, no matter how fast you caught on to grossing and be there for any questions. I start training April 1st and would just like to know what the histo world would expect. Thanks in advance for any opinions. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> earthlink.net Thu Mar 3 09:33:39 2005 From: amosbrooks <@t> earthlink.net (amosbrooks@earthlink.net) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: <29712159.1109864020294.JavaMail.root@statler.psp.pas.earthlink.net> Jeanine, I like to think about it as if I were the patient. If I had a focal lesion and it was on the surface of the block and some one decided that it would be easier to re-embed and re-face the block than to align the microtome and the lesion was faced off, I'd be really upset. Re-embedding the blocks is a rather dumb idea because you need to cut into the block to get a full face anyway, there is bound to be tissue loss. Aligning the block is a really quick process that can be done easily once you are familiar with doing it. You just need to know what to do. Know the tools you are working with and life will be easier for everyone. Re-embedding all the blocks is going to take a lot of time (you certainly aren't saving time with that option) and you are introducing another potential source of problems. What if the cassettes accidentally got switched or tissue from one block got mixed in with tissue from another. It can easily happen when re-embedding a LOT of blocks. It's just not worth it, re-align don't re-embed unless it's absolutly necessary. Just my $0.02 Amos ------------------------------ Message: 3 Date: Wed, 2 Mar 2005 13:42:22 -0500 From: "Bartlett, Jeanine" Subject: [Histonet] block alignment To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 From ree3 <@t> leicester.ac.uk Thu Mar 3 09:49:09 2005 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] UK prices Message-ID: I need to get some idea of how much it costs to process, cut and stain a block of tissue, any help/ideas most gratefully received. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER...U.K....... From JQB7 <@t> CDC.GOV Thu Mar 3 09:54:20 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: I appreciate all of the advice. Amos here is on EXACTLY the same page that I am. But I needed some evidence that I'm on the right track so when I present my concerns at our staff meeting it won't seem like just a difference in technique among techs. Thanks again. Jeanine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amosbrooks@earthlink.net Sent: Thursday, March 03, 2005 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Jeanine, I like to think about it as if I were the patient. If I had a focal lesion and it was on the surface of the block and some one decided that it would be easier to re-embed and re-face the block than to align the microtome and the lesion was faced off, I'd be really upset. Re-embedding the blocks is a rather dumb idea because you need to cut into the block to get a full face anyway, there is bound to be tissue loss. Aligning the block is a really quick process that can be done easily once you are familiar with doing it. You just need to know what to do. Know the tools you are working with and life will be easier for everyone. Re-embedding all the blocks is going to take a lot of time (you certainly aren't saving time with that option) and you are introducing another potential source of problems. What if the cassettes accidentally got switched or tissue from one block got mixed in with tissue from another. It can easily happen when re-embedding a LOT of blocks. It's just not worth it, re-align don't re-embed unless it's absolutly necessary. Just my $0.02 Amos ------------------------------ Message: 3 Date: Wed, 2 Mar 2005 13:42:22 -0500 From: "Bartlett, Jeanine" Subject: [Histonet] block alignment To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Thu Mar 3 10:41:13 2005 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment In-Reply-To: <29712159.1109864020294.JavaMail.root@statler.psp.pas.earthlink.net> Message-ID: <000201c5200f$d040e7c0$3601a8c0@brownpathology.net> I absolutely agree with you!! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amosbrooks@earthlink.net Sent: Thursday, March 03, 2005 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Jeanine, I like to think about it as if I were the patient. If I had a focal lesion and it was on the surface of the block and some one decided that it would be easier to re-embed and re-face the block than to align the microtome and the lesion was faced off, I'd be really upset. Re-embedding the blocks is a rather dumb idea because you need to cut into the block to get a full face anyway, there is bound to be tissue loss. Aligning the block is a really quick process that can be done easily once you are familiar with doing it. You just need to know what to do. Know the tools you are working with and life will be easier for everyone. Re-embedding all the blocks is going to take a lot of time (you certainly aren't saving time with that option) and you are introducing another potential source of problems. What if the cassettes accidentally got switched or tissue from one block got mixed in with tissue from another. It can easily happen when re-embedding a LOT of blocks. It's just not worth it, re-align don't re-embed unless it's absolutly necessary. Just my $0.02 Amos ------------------------------ Message: 3 Date: Wed, 2 Mar 2005 13:42:22 -0500 From: "Bartlett, Jeanine" Subject: [Histonet] block alignment To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Mar 3 10:55:14 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: I use the Collimator made by Microm. Glen Dawson -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 7:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment >From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [ mailto:JQB7@CDC.GOV ] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From David.Edmondson <@t> christie-tr.nwest.nhs.uk Thu Mar 3 11:08:41 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Shandon Hypercenter XP Message-ID: Hi, We have them still running daily , donkey's years old. They have regular maintenance, like yours, valve failure is not something that one can diagnose except that the system just does not run and significant fluid is lost to the drip tray. Now I wonder that the drain times on your programs may be too short and that carry over could be reduced by extending them. They work from 15 secs,(is that right) and you can extend them to a couple of minutes. After the last alcohol ours is say a minute and its longer after the last xylene. And after the waxes because of their greater viscosity. Dave Ed Christie, Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Snider, Deanna Sent: 03 March 2005 14:19 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Shandon Hypercenter XP Shriners Hospital for Children Research Dept. Cincinnatti, Oh 513-872-6388 Hello again everyone in Histoland! Does anyone use this processor? I had not used it prior to accepting this position. What seems to be the problem is that it seems to have a lot of carry over from station to station. I have read the manual, did all the maintenance it suggests, and I diligently keep up the daily routine maintenance. I process very small samples of clinically engineered skin. The largest workload I have ever had here was 34 blocks. Usually it is more like 8- 10 cassettes. I just changed all the reagents yesterday, including both paraffins. I had a run with 4 cassettes overnight and this morning the first paraffin station has a very strong xylene smell. Has anyone else found this to be a problem with this particular piece of equipment? I don't recall having this much of a problem with this in the VIP's or MVPs which I have worked with for the last 15 years. I read through the archives to see if anyone had mentioned this and found nothing, so your comments will be greatly appreciated. Thanks Deanna Snider HT CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From kbradshaw <@t> lcpath.com Thu Mar 3 11:49:40 2005 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] CAP survey slides In-Reply-To: <20050303135931.HMQC19936.fed1rmmtao09.cox.net@smtp.west.cox.net> Message-ID: When doing the IHC survey you stain only the required stain for each case. Example: MK-01; ALK, Pan Keratin,CD3,CD15,CD20,CD30, and LCA. Each case also needs to have an H&E and a negative control. You have been provided with plenty of slides to accomplish everything that is asked and them some extra for redo's if needed. Once all of your stains are completed make a copy of the Survey Result Form and Clinical Histories(pg.4 of Kit Instructions) for each of the pathologists that will be reviewing the slides. The slides will need to be passed from one pathologists to the next, but each pathologists will record their own results on their own form, without sharing results. In our laboratory, the Technical Director compiles the results from everyone who participated and then they are submitted. If you are CAP accredited you should have a policy that outlines how your peer educational program/proficiency testing program operates. GEN.10000;ANP.02000. :) Kari Bradshaw, HT(ASCP) Laboratory Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 (360)425-5620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of ktournear@cox.net Sent: Thursday, March 03, 2005 6:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP survey slids Hi out there, I have the new CAP survey IHC slides for 5 pathologists. My problem is, is that I only have 4 cases/10 slides each case and each pathologist is supposed to do this now. Can anyone tell me how this is done? I don't think I have enough slides for all of them. Thanks!! Kim Tournear, HT (ASCP) QIHC Specialists in Dermatology PLLC Tucson, AZ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> earthlink.net Thu Mar 3 12:07:30 2005 From: marktarango <@t> earthlink.net (Mark Adam Tarango) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Re: Histonet Digest, Vol 16, Issue 7 Message-ID: <15369390.1109873251154.JavaMail.root@scooter.psp.pas.earthlink.net> I started grossing right out of high school. I was 17, but at least I'd already graduated. I had a few months of standing next to the pathologist and handing them all the specimen bottles and closing lids, and one day the pathologist told me "well you've been watching me gross, why don't you just go ahead and do it...call me if you need any help." That was it, he went back into his office, and I just stood there for a few minutes thinking of what to do. After a while I caught on. I know that if that happened today, I'd insist i am not qualified to do gross, but i was younger then. I actually had a job after that one and all I did was gross. They should just have an ASCP grossing qualification or something. Then maybe more people would study / learn how to gross, and less high school seniors would end up doing it. Mark Tarango >Date: Thu, 3 Mar 2005 08:20:38 -0500 >From: "Fawn Jones" >Subject: [Histonet] Training >To: >Message-ID: <915E55B02E236E4D95258B181EEF6317075DC5@namsams01.namsa.int> >Content-Type: text/plain; charset="us-ascii" >I am having a new situation starting to occur, where our company wants >to hire a high school student to help ship our slides and now they are >telling us she can gross our tissues also. We work in a medical device >testing company where we gross everything from muscles, to spines, to >all organs from mice, rats, rabbits, dogs, swine, sheep and guinea pigs. >These are not necessarily easy things to gross as we have to look for >test articles and implant sites (which may not be visible >macroscopically), and determine if there are any changes in the tissues. >I'm not sure we are qualified enough ourselves to be doing these things >let alone a high school senior. If anybody could send any information >on the qualifications needed for this type of work so that we could show >our managers, (which are not histotechs and have no histology >experience), that hiring a high school senior to do grossing is not a >good idea. We also will not have a pathologist on hand to help with >grossing of these tissues or for training. There are no set standards >for grossing as most of our special studies have different test articles >and different things they are looking for, so no two of these studies >are really ever the same. >Thank you in advance >Fawn Jones From jkiernan <@t> uwo.ca Thu Mar 3 12:25:20 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] TH and golgi-cox? References: <20050303014603.93602.qmail@web50104.mail.yahoo.com> Message-ID: <42275690.A36BB5A3@uwo.ca> Golgi-Cox is for showing dendritic structures of individual neurons. It won't help much for recognizing the 6 layers of the cerebral cortex. For this use a Nissl stain, which is any cationic dye with fairly small, flat molecules. Examples are toluidine blue, thionine, cresyl violet and neutral red. The stain should be differentiated until only the Nissl bodies of neurons and the nuclei (most are of glial cells) are coloured. If the tyrosine hydroxylase immunohistochemistry is done with a permanent chromogen such as DAB, do the Nissl stain afterwards, and make it light enough to see the TH+ve fibres. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ ninian H wrote: > > Hi all, > Does anyone know about combining golgi-cox with tyrosine hydroxylase staining? the main purpose of mine is to distinguish different layers of cortex and TH positive fibers. I'll appreciate any information that might help me to see both pyramydal cells and TH containing fibers in cortical areas. > Also co-staining with TH and neutral red didn't work out, and TH -stained neurons didn't stain with red color , any idea or suggestion? > Thank you in advance, > Ninian, ph.D student From Julie.Sanders <@t> med.va.gov Thu Mar 3 12:11:31 2005 From: Julie.Sanders <@t> med.va.gov (Julie.Sanders@med.va.gov) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Survey slide Message-ID: <457381D92B01BD44B21CF37CC02EBDFD02927598@vhacinexc5.v10.med.va.gov> Kim, I have two pathologists and this is how I handle the survey. I copy the results form, one for each, and then give the survey slides to one who then evaluates them, then gives them to the other, they then compare their results and discuss differences to come up with an agreement for diagnosis. Then they mark one of their copies of the survey form with their answers and I transfer the answers to the form I fax back to CAP. Hope this helps. Julie Julie Sanders Supervisor, Anatomic Pathology Cincinnati VAMC Hi out there, I have the new CAP survey IHC slides for 5 pathologists. My problem is, is that I only have 4 cases/10 slides each case and each pathologist is supposed to do this now. Can anyone tell me how this is done? I don't think I have enough slides for all of them. Thanks!! Kim Tournear, HT (ASCP) QIHC Specialists in Dermatology PLLC Tucson, AZ Julie A. Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC From mari.ann.mailhiot <@t> leica-microsystems.com Thu Mar 3 12:43:40 2005 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] RE: disinfection of cryostat Message-ID: Karen As part of our safety campaign, Leica developed guidelines for disinfecting cryostats. They can be applied to any cryostat and we would be happy to share them with you if you are interested. They basically provide instructions for following the CAP guideline requiring that a cryostat be disinfected once a week with a tuberculocidal disinfectant if used daily. A list of approved disinfectants that are effective against various pathogens, including Mycobacteria tuberculosis (tubercle bacteria), human HIV-1 and Hepatitis B virus is available on the EPA website. You can find this list and others at http://www.epa.gov/oppad001/chemregindex.htm. Each disinfecting solution requires exposure to contaminated surfaces for specific times and, unfortunately, none of them are approved for use at cold temperatures. Therefore, the cryostat must be disinfected at room temperature and the directions on the individual solutions must be followed. Needless to say, the least corrosive solution should be selected. It might also be advisable to consult with the cryostat manufacturer for their recommendations. If you chose not to use a tuberculocidal disinfectant, you can use 5% bleach, followed by 70% alcohol (to penetrate the lipid capsule of the tubercle bacteria), followed by 100% alcohol (to remove any water). This procedure must also be done at room temperature and for specific exposure times that are included in our guidelines. Following either procedure, the cryostat will need to be thoroughly dried and lubricated before it is taken back down to operating temperature. If you have any questions or would like a copy of the guidelines, please give me a call here at Leica. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Hopkins, Karen" To: Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] RE: disinfection of cryostat western.edu 03/01/2005 01:12 PM Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From ploykasek <@t> phenopath.com Thu Mar 3 12:49:13 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Survey slide In-Reply-To: <457381D92B01BD44B21CF37CC02EBDFD02927598@vhacinexc5.v10.med.va.gov> Message-ID: Kim, we have 5 pathologists here, and I handle it like Julie. Seems to work for everyone. Patti Loykasek PhenoPath Laboratories Seattle, WA > Kim, > I have two pathologists and this is how I handle the survey. I copy the > results form, one for each, and then give the survey slides to one who then > evaluates them, then gives them to the other, they then compare their > results and discuss differences to come up with an agreement for diagnosis. > Then they mark one of their copies of the survey form with their answers and > I transfer the answers to the form I fax back to CAP. Hope this helps. > Julie > Julie Sanders > Supervisor, Anatomic Pathology > Cincinnati VAMC > Hi out there, > I have the new CAP survey IHC slides for 5 pathologists. My problem is, is > that I only have 4 cases/10 slides each case and each pathologist is > supposed to do this now. Can anyone tell me how this is done? I don't think > I have enough slides for all of them. > Thanks!! > > Kim Tournear, HT (ASCP) QIHC > Specialists in Dermatology PLLC > Tucson, AZ > > Julie A. Sanders, BA,HT(ASCP) > Supervisor, Anatomic Pathology > Cincinnati VAMC > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> earthlink.net Thu Mar 3 13:05:06 2005 From: amosbrooks <@t> earthlink.net (amosbrooks@earthlink.net) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] Re: Histonet Digest, Vol 16, Issue 7 Message-ID: <26901803.1109876707219.JavaMail.root@scooter.psp.pas.earthlink.net> Julie, I strongly reccommend the Immunohistochemical Staining Methods Handbook by DAKO. It should be on their website in .PDF format. Otherwise just call them and they'll send it to you. This is good if you are using any product (not just DAKO) and it gives a great overview of IHC from start to finish. Once you've read that, I would move on to Atlas of Diagnostic Immunopathology by Lawrence True (If you can get a copy, it's hard to find!). This is a fantastic second step book that I always reccommend after the DAKO book. Good luck, Amos Brooks ------------------------------ Message: 5 Date: Thu, 3 Mar 2005 07:46:23 -0600 From: Julie.Sanders@med.va.gov Subject: [Histonet] Basic ISH and IHC To: histonet@lists.utsouthwestern.edu Message-ID: <457381D92B01BD44B21CF37CC02EBDFD02927596@vhacinexc5.v10.med.va.gov> Content-Type: text/plain; charset="iso-8859-1" Hello all, I am looking for any books/information regarding basic (very basic) theories and explanations for ISH and IHC. We do ISH in our lab and know the technique, but not the theory, so I'm trying to find information on these subjects. I've looked at Amazon.com, but most of the books seem way beyond "basic." What we are looking for is something that can give us at least "beginners" information. I spoke to our Ventana rep. and suggested that Ventana should put on regional workshops. (are you reading this Ventana?) With so many histology labs using this technology it would be helpful to have background. Its easy to slap some slides on a machine, put the dispensers on, click start and walk away....now we need to know the how and why we do this. Any information/suggestions would be greatly appreciated! Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VA Medical Center 3200 Vine St. ML113 Cincinnati, Ohio 45220 From funderwood <@t> mcohio.org Thu Mar 3 13:11:32 2005 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] ATP tissue processor Message-ID: Hi All, Any of you have experience with the TBS ATP1 tissue processor? Love to hear your comments, good and bad. Thanks in advance. Fred Underwood Mont. Co. Coroner Dayton, OH From Charles.Embrey <@t> carle.com Thu Mar 3 13:22:41 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:42 2005 Subject: FW: [Histonet] Re: Histonet Digest, Vol 16, Issue 7 Message-ID: -----Original Message----- From: Charles.Embrey Sent: Thursday, March 03, 2005 12:28 PM To: 'Mark Adam Tarango' Subject: RE: [Histonet] Re: Histonet Digest, Vol 16, Issue 7 They do have an "ASCP grossing qualification" it is called PA(ASCP). Check into it, it is a great field. Pathologists' Assistants are finding themselves in great demand. I even now have an open position for another PA to help me out. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Adam Tarango Sent: Thursday, March 03, 2005 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 16, Issue 7 I started grossing right out of high school. I was 17, but at least I'd already graduated. I had a few months of standing next to the pathologist and handing them all the specimen bottles and closing lids, and one day the pathologist told me "well you've been watching me gross, why don't you just go ahead and do it...call me if you need any help." That was it, he went back into his office, and I just stood there for a few minutes thinking of what to do. After a while I caught on. I know that if that happened today, I'd insist i am not qualified to do gross, but i was younger then. I actually had a job after that one and all I did was gross. They should just have an ASCP grossing qualification or something. Then maybe more people would study / learn how to gross, and less high school seniors would end up doing it. Mark Tarango >Date: Thu, 3 Mar 2005 08:20:38 -0500 >From: "Fawn Jones" >Subject: [Histonet] Training >To: >Message-ID: <915E55B02E236E4D95258B181EEF6317075DC5@namsams01.namsa.int> >Content-Type: text/plain; charset="us-ascii" >I am having a new situation starting to occur, where our company wants >to hire a high school student to help ship our slides and now they are >telling us she can gross our tissues also. We work in a medical device >testing company where we gross everything from muscles, to spines, to >all organs from mice, rats, rabbits, dogs, swine, sheep and guinea pigs. >These are not necessarily easy things to gross as we have to look for >test articles and implant sites (which may not be visible >macroscopically), and determine if there are any changes in the tissues. >I'm not sure we are qualified enough ourselves to be doing these things >let alone a high school senior. If anybody could send any information >on the qualifications needed for this type of work so that we could show >our managers, (which are not histotechs and have no histology >experience), that hiring a high school senior to do grossing is not a >good idea. We also will not have a pathologist on hand to help with >grossing of these tissues or for training. There are no set standards >for grossing as most of our special studies have different test articles >and different things they are looking for, so no two of these studies >are really ever the same. >Thank you in advance >Fawn Jones _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Thu Mar 3 13:36:12 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] RE: permeabilization needed in IHC of viral protein? Message-ID: <11ff3fc1200f6b.1200f6b11ff3fc@amc.uva.nl> Hi Carla, Theoretically you don't need permeabilization. Since you have been cutting sections, the cell interior is fully exposed to the antibodies. On top of that you applied a protease step. My advise would be testing the staining under similar conditions without and with saponin 0.1% in all steps (including washing buffers). Again, to my opinion a different staining pattern is not very likely. Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- From Carla M Conway Date Wed, 02 Mar 2005 14:37:50 -0800 To Histonet@lists.utsouthwestern.edu Subject [Histonet] permeabilization needed in IHC of viral protein? Hello, I am interested in using IHC to detect nucleocapsid (N) protein within rhabdovirus. I have had success in detecting the glycoprotein on the envelope surface, however the N protein is located within the virion. Sections (FFPE) will be enzymatically digested with 0.05% protease XIV in TBS according to our standard protocol. Would a permeabilization agent increase access to the interior proteins? Any suggestions/comments will be greatly appreciated. Thank you, Carla Conway Western Fisheries Research Center 6505 NE 65th St Seattle, WA 98115 ph: 206-526-6282 ext. 242 fax: 206-526-6654 From JSCHUMA1 <@t> FAIRVIEW.ORG Thu Mar 3 13:37:37 2005 From: JSCHUMA1 <@t> FAIRVIEW.ORG (JENNIFER SCHUMACHER) Date: Fri Sep 16 15:24:42 2005 Subject: [Histonet] block alignment Message-ID: We currently are using the one marketed by Newcomer, which is the same one that has been previously mentioned as being designed by Mr. Davis in Colorado. They have two separate ones, one which will fit Leica and a different one which will fit Microm. They are not cheap, but it only has to save one specimen to be worth the money. Our techs didn't like them at first (felt they were a waste of money), but have grown to appreciate the importance. I don't have any experience with the Hiso Collimitor. Jennifer Fairview-University Medical Center Minneapolis, MN >>> "Bartlett, Jeanine" 03/03/05 08:14AM >>> The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including “protected health information.” If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> From JQB7 <@t> CDC.GOV Thu Mar 3 13:42:11 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] block alignment Message-ID: The one from Newcomer is just under $800.00 (if memory serves) and the Histo Collimator is around $2500.00. -----Original Message----- From: JENNIFER SCHUMACHER [mailto:JSCHUMA1@FAIRVIEW.ORG] Sent: Thursday, March 03, 2005 2:38 PM To: Bartlett, Jeanine; GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu; JWEEMS@sjha.org Subject: RE: [Histonet] block alignment We currently are using the one marketed by Newcomer, which is the same one that has been previously mentioned as being designed by Mr. Davis in Colorado. They have two separate ones, one which will fit Leica and a different one which will fit Microm. They are not cheap, but it only has to save one specimen to be worth the money. Our techs didn't like them at first (felt they were a waste of money), but have grown to appreciate the importance. I don't have any experience with the Hiso Collimitor. Jennifer Fairview-University Medical Center Minneapolis, MN >>> "Bartlett, Jeanine" 03/03/05 08:14AM >>> The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> From gcallis <@t> montana.edu Thu Mar 3 15:26:18 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Razor Clams In-Reply-To: <7CBBD627E4E688499349A5D11D078316020DBBBC@msgpacpbs.rhq.pac .dfo-mpo.gc.ca> References: <7CBBD627E4E688499349A5D11D078316020DBBBC@msgpacpbs.rhq.pac.dfo-mpo.gc.ca> Message-ID: <6.0.0.22.1.20050303135155.01b37da8@gemini.msu.montana.edu> Access this book: Histological techniques for marine bivalve mollusks. Howard DW , Smith CS. NOAA Technical memorandurm NMFS-F/NEC-25. US Dept of Commerce, National Oceanic and Atmospheric Administration, Woods Hole MA, 1983. I have heard this book has wonderful illustrations and includes techniques. Another book is: Harrison FW et al Microscopic Anatomy of Inverterbrates. Vol 5: Mollusca I and Vol. 6: Mollusca II. Wiley-Liss, New York NY 1991. Also, a simple 0.5 to 0.1% Toludine blue stain applied to a polished edge may also help you out. Any grooves caused by a diamond wafer blade can be gently sanded away with 600 grit paper, rinsed (ultrasonicator get rid of debris), dried and stained. For better depth of stain on calcium bands, simply etch the calcium away for 30 sec to 1 min with 1% formic acid, rinse well with water, blot dry and apply toluidine blue stain for 2 min or so. You will have to determine staining time. IF the stain is too dark, polish it off with 600 grit and stain for shorter time and/or more dilute stain. Diamond saws leave ski tracks - finer grit paper polishes saw marks away. This is similar to surface staining methylmethacrylate embedded thick bone sections that are acid etched and stained with 0.1% toluidine blue in pH 8 0.1M phosphate buffer. One could even take 2 mm thick slices of shell, superglue it to a white plastic slide before polishing, this will stabilize section for all other handling - polishing, staining, and microscopy. Etched sections will show darker bands where calcium resided before acid etching, so you will have darker and lighter areas. For microscopy, sit stained slice on slide (with a 1 1/2 thick coverslip on top of slice, and illuminate with the brightest microscope light possible (no filters), but make sure a clear glass slide has white paper under it to diffuse the light up and around the section rather than light passing through the sample. Coverslip corrects for refractive index so you can view at higher power without a fuzzy image. Viewing at low power is generally not a problem and you may be able to just view a stained edge at lower power and not mess with a coverslip. Coverslipping media is not needed. It may take some playing around to get the results you want, but T blue is simple and cheap. Good luck At 02:48 PM 3/2/2005, you wrote: >Hello Histonetters, > >I have a researcher that is ageing (aging) Razor Clams and would like to >better differentiate the conchiolin, a keratin like substance, versus the >calcium carbonate of the shell. The growth rings consist of alternating >bands of calcium carbonate and conchiolin ( as far as I can find out >conchiolin is keratin "like"). A smooth facet of the shell is produced >after a diamond saw is used to slice it in two. The growth bands are in >the smooth facet but difficult to see. >The bands in older animals are very close together and very difficult to >see. Does anyone have any suggestions re: stains or procedures that might >help better differentiate the bands? >Thanks in advance for any help. > >Cheers >Bill Bennett >Fisheries and Oceans Canada >Pacific Biological Station >Nanaimo, B.C. >Canada >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From JMacDonald <@t> mtsac.edu Thu Mar 3 15:29:53 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:43 2005 Subject: Fw: [Histonet] ASCP testing Message-ID: The ASCP has not made the exam more difficult. In the last two years there has been a record number of people attempting to take the exam to get in before the high school education/2 years experience was eliminated. Many of the people that took the exam and were not successful were not prepared. The ASCP is establishing standards of knowledge for histotechnicians. Feedback and decisions are made by a large number of people, many of whom are not employees of the ASCP, but Histotechnicians/Histotechnologists working in the field. We want to be taken seriously as professionals so we need standards to prove this. I personally don't feel that by requiring a degree or certificate to become a Histotechnician will cause a much greater shortage of HTs. There are many people that are now considering this a career, not just a job. The ASCP has a study guide available for the HT exam. The NSH has self-examination booklets. The ASCP also provides a list of study materials that would benefit an applicant, yet when I have spoken to people that were not successful on the exam many barely studied and others attempted the exam with the thought that since they have been working in a histology lab they know all there is to know. The ASCP is not the reason for the high failure rate. Also to the person who felt that they know all there is to know about fixation - unless you scored 999 on the fixation portion of the ASCP exam you still have more to learn. Jennifer MacDonald -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- To: histonet@pathology.swmed.edu From: "Deltour, Douglas D. (HM2)" Sent by: histonet-bounces@lists.utsouthwestern.edu Date: 03/02/2005 05:44AM Subject: [Histonet] ASCP testing What is alarming to me is that the last exam had a 75% failure percentage(so I was told). Why was there a sudden need for ASCP to make the exam harder then what it used to be (pre-2001-2002)? Is it the almighty dollar? With the new standard in play needing an Associate Degree to be eligible, how will it effect the shortage of "ASCP eligible" techs? I see many going back to school or techs being hired just for ASCP title, not experience. Will this force employers to change there hiring standards or will it be harder to find a ASCP eligible tech to fill a position? For the record I sent my blocks in in 2000 so I have until the end of the year to pass the test. It is kind of discouraging when everyone around you is failing this test 2-3 times. Do I make sense here are am I way off?? As usual :) DOUGLAS D. DELTOUR HISTOLOGY TECHNICIAN NMC PORTSMOUTH VA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Mar 3 15:38:30 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] RE: disinfection of cryostat[Scanned] In-Reply-To: <1030B679AD69D6119C3F00080210DD9D05A3F033@bhrv-nt-11.bhrv.nwest.nhs.uk> Message-ID: Microorganisms will not multipy at -20 but will remain dormant. As soon as they warm up they will continue to do what they did before the deep freeze. Kemlo Rogerson Sent by: histonet-bounces@lists.utsouthwestern.edu 03/01/2005 11:50 PM To 'Charles Scouten' cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] RE: disinfection of cryostat[Scanned] Do bugs grow at minus 20? -----Original Message----- From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: 01 March 2005 20:04 To: Hopkins, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned] Why disinfect the cryostat? Let the durn thing disinfect itself. IF you have this requirement, you may want a self disinfecting cryostat. See the link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=47510 2&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats &idsubcategory=182 Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hopkins, Karen Sent: Tuesday, March 01, 2005 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disinfection of cryostat Does anyone have protocol for disinfection of a Reichert Jung cryostat that would meet CAP requirements for disinfection and not involve removing the microtome from the unit. We would like to use our Reichert daily but it is too involved to disinfect it weekly (bringing the unit to room temp, removing the microtome etc...) I like the Shandons method using formaldehyde but do not know if I could get away with just wiping the insides out with formaldehyde or not? Please offer any advise you think may help.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.schmidt <@t> fuse.net Thu Mar 3 17:18:06 2005 From: a.schmidt <@t> fuse.net (Angela M. Schmidt) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Ventana workshops Message-ID: <20050303231618.EKCE20940.gx5.fuse.net@smtp.fuse.net> Julie, I think that it would be great if Ventana could put on a basic IHC and ISH workshop. We have their technology but understanding the basics in this growing part of our field would be greatly appreciated!! Ventana---Southwest OHIO needs you!!! Angela M. Schmidt HT(ASCP) Lead Histology Technician TriHealth Laboratories Good Samaritan-Bethesda Hospitals Cincinnati, Ohio 513-872-1508 a.schmidt@fuse.net From herme013 <@t> umn.edu Thu Mar 3 17:26:35 2005 From: herme013 <@t> umn.edu (Yves Heremans) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] lymphocyte markers NOD vs Balb c Message-ID: <91e91b43f48eb79999dc705c7edd4318@umn.edu> Dear All, Is anybody aware of lymphocyte markers which can distinguish between NOD and Balb c ? Preferably to be used for staining ? Tx. Yves Yves Heremans University of Minnesota Stem Cell Institute Tel 612-625-0964 Fax 612-624-2436 Address for US Postal Mail: University of Minnesota MMC 716 420 Delaware Street SE Minneapolis, MN 55455 Address for COURIER DELIVERY: Suite 14-285 Moos Tower 515 Delaware Street SE From csawrenk <@t> bccancer.bc.ca Thu Mar 3 17:34:30 2005 From: csawrenk <@t> bccancer.bc.ca (Sawrenko, Christina) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] xylene substitute Message-ID: <57AD92D1EDD1854BAAC02DD7DDAF0E360170055F@srvex10.phsabc.ehcnet.ca> Good afternoon all, Does anyone out there have any experience with a product "Formula 83", a xylene substitute, from CJB Biotech? We are especially interested in any effect on immunohistochemistry. Thanks in advance, Chris Sawrenko Histopathology BC Cancer Agency Vancouver, British Columbia, Canada From Krat18 <@t> aol.com Thu Mar 3 17:49:54 2005 From: Krat18 <@t> aol.com (Krat18@aol.com) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] xylene substitute Message-ID: No, but I'd be very interested to learn what you find out. We've received a sample of it and have not yet had a chance to try it ---- that is my main concern, the effect on IHC. So keep us informed on the 'net. Thanks. _Karen_Raterman@ssmhc.com_ (mailto:Karen_Raterman@ssmhc.com) From portera203 <@t> yahoo.com Thu Mar 3 18:34:23 2005 From: portera203 <@t> yahoo.com (Amy Porter) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Basic ISH and IHC In-Reply-To: 6667 Message-ID: <20050304003423.98966.qmail@web40912.mail.yahoo.com> Dako sells a nice very basic handbook for IHC it is now in it's 3rd edition. I believe it is also downloadable in pdf format from their website. Julie.Sanders@med.va.gov wrote: Hello all, I am looking for any books/information regarding basic (very basic) theories and explanations for ISH and IHC. We do ISH in our lab and know the technique, but not the theory, so I'm trying to find information on these subjects. I've looked at Amazon.com, but most of the books seem way beyond "basic." What we are looking for is something that can give us at least "beginners" information. I spoke to our Ventana rep. and suggested that Ventana should put on regional workshops. (are you reading this Ventana?) With so many histology labs using this technology it would be helpful to have background. Its easy to slap some slides on a machine, put the dispensers on, click start and walk away....now we need to know the how and why we do this. Any information/suggestions would be greatly appreciated! Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VA Medical Center 3200 Vine St. ML113 Cincinnati, Ohio 45220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Amy S.Porter, HT(ASCP), QIHC Michigan State University - Department of Physiology Division of Human Pathology Investigative Histopathology Laboratory 2201 Biomedical Physical Sciences Room 2133 East Lansing, MI 48824-3320 Ph: 517-355-6475 ext 1480/1481 Fax: 517-432-1368 portera@msu.edu --------------------------------- Celebrate Yahoo!'s 10th Birthday! Yahoo! Netrospective: 100 Moments of the Web From ktournear <@t> cox.net Thu Mar 3 19:19:58 2005 From: ktournear <@t> cox.net (ktournear@cox.net) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] CAP survey slides Message-ID: <20050304011959.TRAE19936.fed1rmmtao09.cox.net@smtp.west.cox.net> Thanks to all who repsonded to my question regarding the CAP survey. Looks like everyone is doing just about the same thing. Again, thank you so much!!!! Kim Tournear, HT (ASCP) QIHC Specialists in Dermatology PLLC Tucson, AZ From Kemlo.Rogerson <@t> elht.nhs.uk Fri Mar 4 01:49:03 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] UK prices[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F04D@bhrv-nt-11.bhrv.nwest.nhs.uk> Some years ago it was ?5 for processing, cutting and staining an H&E. -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: 03 March 2005 15:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] UK prices[Scanned] I need to get some idea of how much it costs to process, cut and stain a block of tissue, any help/ideas most gratefully received. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER...U.K....... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsukumar <@t> dpspa.com Fri Mar 4 04:55:33 2005 From: rsukumar <@t> dpspa.com (Ray Sukumar) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 7 In-Reply-To: <20050303165237.1DB7E34031@cmlapp23.siteprotect.com> Message-ID: How do we advertize for a Histology Manager and a histotech at your website? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, March 03, 2005 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Need an opinion[Scanned] (Kemlo Rogerson) 2. Training (Fawn Jones) 3. RE: block alignment (Dawson, Glen) 4. RE: block alignment (Weems, Joyce) 5. Basic ISH and IHC (Julie.Sanders@med.va.gov) 6. RE: block alignment (Bartlett, Jeanine) 7. CAP survey slids (ktournear@cox.net) 8. RE: block alignment (Weems, Joyce) 9. Shandon Hypercenter XP (Snider, Deanna) 10. RE: block alignment (Bartlett, Jeanine) 11. Re: Basic ISH and IHC (Kelly D Mcqueeney) 12. Re: TH and golgi-cox? (Geoff McAuliffe) 13. RE: block alignment (Weems, Joyce) 14. RE: block alignment (Bartlett, Jeanine) 15. RE: Need an opinion (Charles.Embrey) 16. block alignment (amosbrooks@earthlink.net) 17. UK prices (Edwards, R.E.) 18. RE: block alignment (Bartlett, Jeanine) 19. RE: block alignment (Bonnie Whitaker) ---------------------------------------------------------------------- Message: 1 Date: Thu, 3 Mar 2005 12:36:57 -0000 From: Kemlo Rogerson Subject: RE: [Histonet] Need an opinion[Scanned] To: "'Heather.A.Harper@pcola.med.navy.mil'" , histonet@lists.utsouthwestern.edu Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F04C@bhrv-nt-11.bhrv.nwest.nhs.uk> Content-Type: text/plain You need at the very least a Standard Operational procedure for 'grossing'. This will have each specimen and how the Pathologist has trained you to 'cut it'. For example skin biopsies of a ?mole, would be cut TS with three slices across the mole. The ends retained 'just in case'. The same for anything else you dissect or 'put in'. That is each sample with have a procedure for you to follow, which you do with no alteration. I would have thought that if a Pathologist sat in for a few days whilst you carried out the 'cut up' then you would learn quickly; if I was the Pathologist then I would occasionally revisit to make sure. It really depends on your experience and training; the issues relating to tissue 'cross over' (on forceps, cut up board, etc) are maybe the most important, but other issues concerning under fixed tissue and your ability to know when to ask for help given an unusually finding need to be addressed. The latter is one of the most important; you can train people to do almost anything, the trick is to get them to recognise when they are 'stepping out' of that training (i.e. it's not a mole, but the Clinical data and gross macro suggests that a melanoma may be a likelihood, then you refer). Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Heather.A.Harper@pcola.med.navy.mil [mailto:Heather.A.Harper@pcola.med.navy.mil] Sent: 03 March 2005 11:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need an opinion[Scanned] I had written last week in regards to the sudden work change of me having to learn how to gross. I'm going to go ahead and learn but I need an opinion. I have 30 days to learn how to gross. Presently, the pathologist is training a cyto tech to gross. She got trained last week and this week is flying solo and she is very unsure of what she is doing and I have been telling her what she has to do. She already lost 2 cxbx on a case. I also observed this same type of training with my military co-worker. Trained one week, flying solo the next and that particular pathologist isn't always in his office. So she would have to de-glove and pick up the phone and call a pathologist. If you were in my situation, tell me in your opinion what 30 days of training would entail because maybe I'm expecting too much. I believe for those 30 days, the pathologist should be standing in the background, watching, listening and observing, no matter how fast you caught on to grossing and be there for any questions. I start training April 1st and would just like to know what the histo world would expect. Thanks in advance for any opinions. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 3 Mar 2005 08:20:38 -0500 From: "Fawn Jones" Subject: [Histonet] Training To: Message-ID: <915E55B02E236E4D95258B181EEF6317075DC5@namsams01.namsa.int> Content-Type: text/plain; charset="us-ascii" I am having a new situation starting to occur, where our company wants to hire a high school student to help ship our slides and now they are telling us she can gross our tissues also. We work in a medical device testing company where we gross everything from muscles, to spines, to all organs from mice, rats, rabbits, dogs, swine, sheep and guinea pigs. These are not necessarily easy things to gross as we have to look for test articles and implant sites (which may not be visible macroscopically), and determine if there are any changes in the tissues. I'm not sure we are qualified enough ourselves to be doing these things let alone a high school senior. If anybody could send any information on the qualifications needed for this type of work so that we could show our managers, (which are not histotechs and have no histology experience), that hiring a high school senior to do grossing is not a good idea. We also will not have a pathologist on hand to help with grossing of these tissues or for training. There are no set standards for grossing as most of our special studies have different test articles and different things they are looking for, so no two of these studies are really ever the same. Thank you in advance Fawn Jones ------------------------------ Message: 3 Date: Thu, 3 Mar 2005 07:27:43 -0600 From: "Dawson, Glen" Subject: RE: [Histonet] block alignment To: "Bartlett, Jeanine" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 3 Mar 2005 08:44:18 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] block alignment To: "Dawson, Glen" , "Bartlett, Jeanine" , Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D1DD@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" >From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 5 Date: Thu, 3 Mar 2005 07:46:23 -0600 From: Julie.Sanders@med.va.gov Subject: [Histonet] Basic ISH and IHC To: histonet@lists.utsouthwestern.edu Message-ID: <457381D92B01BD44B21CF37CC02EBDFD02927596@vhacinexc5.v10.med.va.gov> Content-Type: text/plain; charset="iso-8859-1" Hello all, I am looking for any books/information regarding basic (very basic) theories and explanations for ISH and IHC. We do ISH in our lab and know the technique, but not the theory, so I'm trying to find information on these subjects. I've looked at Amazon.com, but most of the books seem way beyond "basic." What we are looking for is something that can give us at least "beginners" information. I spoke to our Ventana rep. and suggested that Ventana should put on regional workshops. (are you reading this Ventana?) With so many histology labs using this technology it would be helpful to have background. Its easy to slap some slides on a machine, put the dispensers on, click start and walk away....now we need to know the how and why we do this. Any information/suggestions would be greatly appreciated! Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VA Medical Center 3200 Vine St. ML113 Cincinnati, Ohio 45220 ------------------------------ Message: 6 Date: Thu, 3 Mar 2005 08:48:54 -0500 From: "Bartlett, Jeanine" Subject: RE: [Histonet] block alignment To: "Weems, Joyce" , "Dawson, Glen" , Message-ID: Content-Type: text/plain; charset="us-ascii" Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 7 Date: Thu, 3 Mar 2005 8:59:31 -0500 From: Subject: [Histonet] CAP survey slids To: Message-ID: <20050303135931.HMQC19936.fed1rmmtao09.cox.net@smtp.west.cox.net> Content-Type: text/plain; charset=ISO-8859-1 Hi out there, I have the new CAP survey IHC slides for 5 pathologists. My problem is, is that I only have 4 cases/10 slides each case and each pathologist is supposed to do this now. Can anyone tell me how this is done? I don't think I have enough slides for all of them. Thanks!! Kim Tournear, HT (ASCP) QIHC Specialists in Dermatology PLLC Tucson, AZ ------------------------------ Message: 8 Date: Thu, 3 Mar 2005 09:10:41 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] block alignment To: "Bartlett, Jeanine" , "Dawson, Glen" , Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D1DF@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 9 Date: Thu, 3 Mar 2005 09:19:23 -0500 From: "Snider, Deanna" Subject: [Histonet] Shandon Hypercenter XP To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Shriners Hospital for Children Research Dept. Cincinnatti, Oh 513-872-6388 Hello again everyone in Histoland! Does anyone use this processor? I had not used it prior to accepting this position. What seems to be the problem is that it seems to have a lot of carry over from station to station. I have read the manual, did all the maintenance it suggests, and I diligently keep up the daily routine maintenance. I process very small samples of clinically engineered skin. The largest workload I have ever had here was 34 blocks. Usually it is more like 8- 10 cassettes. I just changed all the reagents yesterday, including both paraffins. I had a run with 4 cassettes overnight and this morning the first paraffin station has a very strong xylene smell. Has anyone else found this to be a problem with this particular piece of equipment? I don't recall having this much of a problem with this in the VIP's or MVPs which I have worked with for the last 15 years. I read through the archives to see if anyone had mentioned this and found nothing, so your comments will be greatly appreciated. Thanks Deanna Snider HT CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. ------------------------------ Message: 10 Date: Thu, 3 Mar 2005 09:14:47 -0500 From: "Bartlett, Jeanine" Subject: RE: [Histonet] block alignment To: "Weems, Joyce" , "Dawson, Glen" , Message-ID: Content-Type: text/plain; charset="us-ascii" The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 11 Date: Thu, 03 Mar 2005 09:18:09 -0500 From: Kelly D Mcqueeney Subject: Re: [Histonet] Basic ISH and IHC To: Julie.Sanders@med.va.gov Cc: histonet@lists.utsouthwestern.edu Message-ID: <42271CA1.2020001@bms.com> Content-Type: text/plain; charset=us-ascii; format=flowed Try the Roche website, it's pretty informative http://us.diagnostics.roche.com/ click on *research* then *printed materials* then *non-radioactive In Situ Hybridization Application Manual. *They give a nice description of the experiment, reagents, and WHY you use them. * For IHC, try *Current Protocols in Immunology. * http://www.does.org/masterli/cpi.html * Julie.Sanders@med.va.gov wrote: >Hello all, >I am looking for any books/information regarding basic (very basic) theories >and explanations for ISH and IHC. We do ISH in our lab and know the >technique, but not the theory, so I'm trying to find information on these >subjects. I've looked at Amazon.com, but most of the books seem way beyond >"basic." What we are looking for is something that can give us at least >"beginners" information. I spoke to our Ventana rep. and suggested that >Ventana should put on regional workshops. (are you reading this Ventana?) >With so many histology labs using this technology it would be helpful to >have background. Its easy to slap some slides on a machine, put the >dispensers on, click start and walk away....now we need to know the how and >why we do this. Any information/suggestions would be greatly appreciated! > >Julie Sanders, BA, HT(ASCP) >Supervisor, Anatomic Pathology >VA Medical Center >3200 Vine St. ML113 >Cincinnati, Ohio 45220 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 12 Date: Thu, 03 Mar 2005 09:43:29 -0800 From: Geoff McAuliffe Subject: Re: [Histonet] TH and golgi-cox? To: ninian H Cc: histonet@lists.utsouthwestern.edu Message-ID: <42274CC1.7090405@umdnj.edu> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Hi Ninian: As for Golgi-Cox, John Kiernan is the man to ask. As for a nuclear stain to go with TH, I have used Toluidine Blue, Thionin, Cresyl Violet and Celestine Blue B with excellent results. Assuming you are demonstrating TH with DAB, get some of Vector's "Intense", it turns the DAB a nice golden color which goes very well with any blue nuclear stain. If you are intensifying the DAB with nickel+cobalt a red nuclear stain would look nice. I don't know why neutral red did not work for you, I suggest a fresh batch. Or try "Scarba Red", a neutral red variant. See Slidders et al., J. Pathol. Bacterol. 75:476-478, 1958. Geoff ninian H wrote: >Hi all, >Does anyone know about combining golgi-cox with tyrosine hydroxylase staining? the main purpose of mine is to distinguish different layers of cortex and TH positive fibers. I'll appreciate any information that might help me to see both pyramydal cells and TH containing fibers in cortical areas. >Also co-staining with TH and neutral red didn't work out, and TH -stained neurons didn't stain with red color , any idea or suggestion? >Thank you in advance, >Ninian, ph.D student > > >histonet-request@lists.utsouthwestern.edu wrote: >Send Histonet mailing list submissions to >histonet@lists.utsouthwesten.edu > >To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to >histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at >histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > >1. RE: removal of carbohydrates/glycosylation >(Edmondson David (RBV) NHS Christie Tr) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Wed, 2 Mar 2005 17:48:49 -0000 >From: "Edmondson David \(RBV\) NHS Christie Tr" > >Subject: RE: [Histonet] removal of carbohydrates/glycosylation >To: "Sharon Cooperman" >Cc: "Histonet \(E-mail 2\)" >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > > >I wonder that oxidation will do this but that there will be consequences for the antigens that you are working with. Years ago, I remember using Periodic acid and then Borohydride as a peroxidase block but that PD726 samples from Oxford did not work at all. The LCA is obviously a run of the mill antibody now and carbohydrate moities a significant part thereof. >Any suggestions/ > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sharon >Cooperman >Sent: 02 March 2005 15:02 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] removal of carbohydrates/glycosylation > > >Hi, > >Does anyone know of a method to remove carbohydrate >modifications/glycosylation from proteins in tissue sections on >slides prior to immunohistochemistry? (I know it's a weird >question.) Any suggestions, references, etc. would be greatly >appreciated. > >Thanks, >Sharon > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 13 Date: Thu, 3 Mar 2005 09:39:44 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] block alignment To: "Bartlett, Jeanine" , "Dawson, Glen" , Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D1E0@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" Unless it's changed, it doesn't fit Leicas. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 9:14 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 14 Date: Thu, 3 Mar 2005 09:43:40 -0500 From: "Bartlett, Jeanine" Subject: RE: [Histonet] block alignment To: "Weems, Joyce" , "Dawson, Glen" , Message-ID: Content-Type: text/plain; charset="us-ascii" The Histo Collimator doesn't, but the one from Newcomer does. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:40 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Unless it's changed, it doesn't fit Leicas. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 9:14 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 15 Date: Thu, 3 Mar 2005 08:51:08 -0600 From: "Charles.Embrey" Subject: RE: [Histonet] Need an opinion To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Just for a comparison..... Pathologists' Assistants study two years to complete their degree to be able to gross. One week hardly seems adequate. Your Doc's must be glad that it is near to impossible to sue military doctors for malpractice. Poor patients. Charles Embrey -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Thursday, March 03, 2005 5:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need an opinion I had written last week in regards to the sudden work change of me having to learn how to gross. I'm going to go ahead and learn but I need an opinion. I have 30 days to learn how to gross. Presently, the pathologist is training a cyto tech to gross. She got trained last week and this week is flying solo and she is very unsure of what she is doing and I have been telling her what she has to do. She already lost 2 cxbx on a case. I also observed this same type of training with my military co-worker. Trained one week, flying solo the next and that particular pathologist isn't always in his office. So she would have to de-glove and pick up the phone and call a pathologist. If you were in my situation, tell me in your opinion what 30 days of training would entail because maybe I'm expecting too much. I believe for those 30 days, the pathologist should be standing in the background, watching, listening and observing, no matter how fast you caught on to grossing and be there for any questions. I start training April 1st and would just like to know what the histo world would expect. Thanks in advance for any opinions. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 3 Mar 2005 10:33:39 -0500 (GMT-05:00) From: amosbrooks@earthlink.net Subject: [Histonet] block alignment To: histonet@lists.utsouthwestern.edu Message-ID: <29712159.1109864020294.JavaMail.root@statler.psp.pas.earthlink.net> Content-Type: text/plain; charset=us-ascii Jeanine, I like to think about it as if I were the patient. If I had a focal lesion and it was on the surface of the block and some one decided that it would be easier to re-embed and re-face the block than to align the microtome and the lesion was faced off, I'd be really upset. Re-embedding the blocks is a rather dumb idea because you need to cut into the block to get a full face anyway, there is bound to be tissue loss. Aligning the block is a really quick process that can be done easily once you are familiar with doing it. You just need to know what to do. Know the tools you are working with and life will be easier for everyone. Re-embedding all the blocks is going to take a lot of time (you certainly aren't saving time with that option) and you are introducing another potential source of problems. What if the cassettes accidentally got switched or tissue from one block got mixed in with tissue from another. It can easily happen when re-embedding a LOT of blocks. It's just not worth it, re-align don't re-embed unless it's absolutly necessary. Just my $0.02 Amos ------------------------------ Message: 3 Date: Wed, 2 Mar 2005 13:42:22 -0500 From: "Bartlett, Jeanine" Subject: [Histonet] block alignment To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 ------------------------------ Message: 17 Date: Thu, 3 Mar 2005 15:49:09 -0000 From: "Edwards, R.E." Subject: [Histonet] UK prices To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I need to get some idea of how much it costs to process, cut and stain a block of tissue, any help/ideas most gratefully received. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER...U.K....... ------------------------------ Message: 18 Date: Thu, 3 Mar 2005 10:54:20 -0500 From: "Bartlett, Jeanine" Subject: RE: [Histonet] block alignment To: , Message-ID: Content-Type: text/plain; charset="us-ascii" I appreciate all of the advice. Amos here is on EXACTLY the same page that I am. But I needed some evidence that I'm on the right track so when I present my concerns at our staff meeting it won't seem like just a difference in technique among techs. Thanks again. Jeanine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amosbrooks@earthlink.net Sent: Thursday, March 03, 2005 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Jeanine, I like to think about it as if I were the patient. If I had a focal lesion and it was on the surface of the block and some one decided that it would be easier to re-embed and re-face the block than to align the microtome and the lesion was faced off, I'd be really upset. Re-embedding the blocks is a rather dumb idea because you need to cut into the block to get a full face anyway, there is bound to be tissue loss. Aligning the block is a really quick process that can be done easily once you are familiar with doing it. You just need to know what to do. Know the tools you are working with and life will be easier for everyone. Re-embedding all the blocks is going to take a lot of time (you certainly aren't saving time with that option) and you are introducing another potential source of problems. What if the cassettes accidentally got switched or tissue from one block got mixed in with tissue from another. It can easily happen when re-embedding a LOT of blocks. It's just not worth it, re-align don't re-embed unless it's absolutly necessary. Just my $0.02 Amos ------------------------------ Message: 3 Date: Wed, 2 Mar 2005 13:42:22 -0500 From: "Bartlett, Jeanine" Subject: [Histonet] block alignment To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 3 Mar 2005 10:41:13 -0600 From: "Bonnie Whitaker" Subject: RE: [Histonet] block alignment To: , Message-ID: <000201c5200f$d040e7c0$3601a8c0@brownpathology.net> Content-Type: text/plain; charset="us-ascii" I absolutely agree with you!! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amosbrooks@earthlink.net Sent: Thursday, March 03, 2005 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Jeanine, I like to think about it as if I were the patient. If I had a focal lesion and it was on the surface of the block and some one decided that it would be easier to re-embed and re-face the block than to align the microtome and the lesion was faced off, I'd be really upset. Re-embedding the blocks is a rather dumb idea because you need to cut into the block to get a full face anyway, there is bound to be tissue loss. Aligning the block is a really quick process that can be done easily once you are familiar with doing it. You just need to know what to do. Know the tools you are working with and life will be easier for everyone. Re-embedding all the blocks is going to take a lot of time (you certainly aren't saving time with that option) and you are introducing another potential source of problems. What if the cassettes accidentally got switched or tissue from one block got mixed in with tissue from another. It can easily happen when re-embedding a LOT of blocks. It's just not worth it, re-align don't re-embed unless it's absolutly necessary. Just my $0.02 Amos ------------------------------ Message: 3 Date: Wed, 2 Mar 2005 13:42:22 -0500 From: "Bartlett, Jeanine" Subject: [Histonet] block alignment To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 7 *************************************** From r.e.hands <@t> qmul.ac.uk Fri Mar 4 06:10:32 2005 From: r.e.hands <@t> qmul.ac.uk (Rebecca Hands) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] OCT embedding... Message-ID: <00f301c520b3$2a185640$b886258a@mds.qmw.ac.uk> Dear All, I am currently using OCT as an embedding medium for fresh frozen tissue. Ideally i need to cut sections from this embedded tissue, laser dissect specific areas of interest and extract high quality RNA from the lasered tissue for subsequent RT-PCR and microarray analysis. Does anyone know if OCT medium degrades RNA?...if so can RNase inhibitors be added to such solutions? Also does the actual embedding procedure itself comprise RNA integrity?...does anyone have a good protocol for this techniwque and subsequent good quality RNA extraction? Regards Rebecca Rebecca Hands (MSc) PhD Research Fellow Academic Dept of Surgery 4th Floor Alexandra Wing The Royal London Hospital Whitechapel Road Whitechapel London E1 1BB Ext 2614 From BWinters <@t> NCH.ORG Fri Mar 4 06:52:11 2005 From: BWinters <@t> NCH.ORG (Winters, Bert) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] PART TIME HISTOTECHNOLOGIST Message-ID: <270614B321ACB44D8C1D91F4F921FDC362779A@NCH01EX02.nch.org> We are currently looking for a part time histotech to work in our laboratory. We are looking for a 3'00 am to 7 am tech to embed and cut biopsy specimens . Anyone interested can contact me in care of: Bert Winters, Histology supervisor 800 West central road, Arlington Heights, Ill. 60005 Phone 847-618-6190 E-mail: BWINTERS@NCH.ORG ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From JNocito <@t> Pathreflab.com Fri Mar 4 07:44:32 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Need an opinion In-Reply-To: Message-ID: Heather, I've been grossing for four years and I'm still learning. I'm trying to get accepted to take the PA exam this year. like Chuck said, most PA programs are two years. I think the pass rate for OJT trained PAs is 11%. I have a large mountain to climb even though I have 25 years experience in the field. One month is not adequate enough. Hell, pathology residents get more training than that. Joe Nocito Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Thursday, March 03, 2005 8:51 AM To: Heather.A.Harper@pcola.med.navy.mil; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Need an opinion Just for a comparison..... Pathologists' Assistants study two years to complete their degree to be able to gross. One week hardly seems adequate. Your Doc's must be glad that it is near to impossible to sue military doctors for malpractice. Poor patients. Charles Embrey -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Thursday, March 03, 2005 5:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need an opinion I had written last week in regards to the sudden work change of me having to learn how to gross. I'm going to go ahead and learn but I need an opinion. I have 30 days to learn how to gross. Presently, the pathologist is training a cyto tech to gross. She got trained last week and this week is flying solo and she is very unsure of what she is doing and I have been telling her what she has to do. She already lost 2 cxbx on a case. I also observed this same type of training with my military co-worker. Trained one week, flying solo the next and that particular pathologist isn't always in his office. So she would have to de-glove and pick up the phone and call a pathologist. If you were in my situation, tell me in your opinion what 30 days of training would entail because maybe I'm expecting too much. I believe for those 30 days, the pathologist should be standing in the background, watching, listening and observing, no matter how fast you caught on to grossing and be there for any questions. I start training April 1st and would just like to know what the histo world would expect. Thanks in advance for any opinions. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lwhite <@t> lakeridgehealth.on.ca Fri Mar 4 07:46:55 2005 From: lwhite <@t> lakeridgehealth.on.ca (White, Lori) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] RE: Antibody diluting buffer Message-ID: Barb, Please forward your message to Dakocytomation. I have been also using their diluting buffer for years and have tried both of their alternatives and am very unhappy! It is unfortunate that the product was switched without polling their current users (I think there are a fair number that used the original buffer). I also think they need to know the customers are unhappy and are looking for alternatives. Lori White, Oshawa, Ontario Message: 2 Date: Tue, 01 Mar 2005 13:26:24 -0500 From: "B T" Subject: [Histonet] antibody diluting buffer To: histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; format=flowed Hello all, Apparantly Dakocytomation no longer has the antibody diluting buffer available that we have been using for many years. This is truely unfortunate as we have found it to be extremely stable over long periods of time. The new product they are forced to offer is not very stable, and unsuitable for our purposes. I know other companies must offer a similar product, so I am interested in what other people are using, from what company, and how long it seems to remain stable. Thanks so much for your input. Barb ------------------------------ From sverma8 <@t> lycos.com Fri Mar 4 08:04:19 2005 From: sverma8 <@t> lycos.com (Subhash Verma) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] PNA staining Message-ID: <20050304140419.64AE7CA06F@ws7-4.us4.outblaze.com> Hello there, I wish to carry out PNA staining on formalin-fixed paraffin embedded section (as I did not cryopreserve them) from lymph nodes and spleen to distinguish between primary lymphoid follicles and secondary lymphoid follicles. Will it be possible to use PNA staining on formalin-fixed paraffin embedded section and will I be able to distuish between primary lymphoid follicles and secondary lymphoid follicles easily as opposed to looking on H&E stained sections. Thanks for your help. S Verma -- _______________________________________________ NEW! Lycos Dating Search. The only place to search multiple dating sites at once. http://datingsearch.lycos.com From la.sebree <@t> hosp.wisc.edu Fri Mar 4 08:28:21 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Ventana workshops Message-ID: Angela, We have found Ventana very receptive to such requests. They are more than happy to provide speakers/workshop leaders for state society meetings. So I suggest you contact them before your next meeting to see if something can be arranged. I know Ethel Macrea is an excellent, experienced speaker for just these sort of situations. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela M. Schmidt Sent: Thursday, March 03, 2005 5:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana workshops Julie, I think that it would be great if Ventana could put on a basic IHC and ISH workshop. We have their technology but understanding the basics in this growing part of our field would be greatly appreciated!! Ventana---Southwest OHIO needs you!!! Angela M. Schmidt HT(ASCP) Lead Histology Technician TriHealth Laboratories Good Samaritan-Bethesda Hospitals Cincinnati, Ohio 513-872-1508 a.schmidt@fuse.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Fri Mar 4 08:43:19 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] RE: Learning to gross in 30 days Message-ID: <20050304144319.68649.qmail@web30406.mail.mud.yahoo.com> Dear Heather, You are being asked to learn how to gross in 30 days. It is certainly possible to learn to put through biopsies, currettings, small orthopedic specimens, and a variety of small insignificant specimens where your task is limited to counting, measuring, describing and cassetting these samples. To learn to properly gross large specimens such as breasts, colons, laryngectomies, prostates, bladders, uteri, and the like it requires considerably more knowledge. You really need to have knowledge of pathology. A simple way to approach these specimens is to think about them in two ways: Anatomically and clinically. Speaking anatomically, you must know the anatomy and histology of the normal organ in order to recognize the pathology. All pathologic changes must be described in terms of size, shape, color, consistency, distance from anatomic landmarks, patterns of growth, relation to adjacent organs. You must be able to dissect and section each organ in the accepted way. To do this properly you must also have knowledge of the various disease processes which affect each organ. Speaking clinically you need to know what the clinician requires in order to treat the patient. You have to be familiar with the staging of each type of tumor and organ, so you can describe the pathology and take all of the sections correctly. In addition you must be skilled with dissection, and be able to cut your sections a uniform 3 mm thick. This is far more than I could learn in a month. I recommend Dr Westra's book Surgical Pathology Dissection as a good starting point. Start with only small simple specimens. Begin to learn the large specimens one at a time. Familiarize yourself with the anatomy and histology. Start with a book like this which will describe the approach to dissection and will give you the important clinical information and which sections to take. Make sure you ask for help from you pathologists on anything you are unsure of. Learning to be a PA is a two year program. I have trained a number of PA's on the job but they were all foreign MD's. And after years they still continue to need instruction. Take it slow, and ask for help. You would not want to do any patient a disservice. If you manage to pull it off you will have learned a job that is highly sought after and pays nicely. But if you are going to do it, do it right or you will loose a lot of sleep at nites when mistakes start rearing there ugly heads. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From ryaskovich <@t> dir.nidcr.nih.gov Fri Mar 4 08:46:32 2005 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR)) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] RE: Antibody diluting buffer Message-ID: <8F3AB322628548428A992EFB0E80F5D315D63064@nihexchange8.nih.gov> I spoke to my rep. at Dako Michelle Okonski this week and she said some items were going to be discontinued as far as she knows Antibody Diluent w/ Background Reducing Components #S3022 was not one of them. I don't know if that's the one everyone needs to know about or not. Michelle is still checking on the items for us. Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Drug Discovery Pain Branch > ---------- > From: White, Lori > Sent: Friday, March 4, 2005 7:46 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Antibody diluting buffer > > > Barb, > Please forward your message to Dakocytomation. I have been also using > their diluting buffer for years and have tried both of their > alternatives and am very unhappy! It is unfortunate that the product > was switched without polling their current users (I think there are a > fair number that used the original buffer). I also think they need to > know the customers are unhappy and are looking for alternatives. > > Lori White, > Oshawa, Ontario > > > > > Message: 2 > Date: Tue, 01 Mar 2005 13:26:24 -0500 > From: "B T" > Subject: [Histonet] antibody diluting buffer > To: histonet@pathology.swmed.edu > Message-ID: > Content-Type: text/plain; format=flowed > > Hello all, > > Apparantly Dakocytomation no longer has the antibody diluting buffer > available that we have been using for many years. This is truely > unfortunate > as we have found it to be extremely stable over long periods of time. > The > new product they are forced to offer is not very stable, and unsuitable > for > our purposes. I know other companies must offer a similar product, so I > am > interested in what other people are using, from what company, and how > long > it seems to remain stable. > > Thanks so much for your input. > > Barb > > > > > > ------------------------------ > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From HornHV <@t> archildrens.org Fri Mar 4 08:49:18 2005 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] block alignment Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6322BAA6@EMAIL.archildrens.org> Can someone tell how this device works? I have never heard of it. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital - Changing Children's Lives Phone - 501.364.4240 Fax - 501.364.3912 Visit us on the web at www. archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Thursday, March 03, 2005 1:42 PM To: JENNIFER SCHUMACHER; GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu; JWEEMS@sjha.org Subject: RE: [Histonet] block alignment The one from Newcomer is just under $800.00 (if memory serves) and the Histo Collimator is around $2500.00. -----Original Message----- From: JENNIFER SCHUMACHER [mailto:JSCHUMA1@FAIRVIEW.ORG] Sent: Thursday, March 03, 2005 2:38 PM To: Bartlett, Jeanine; GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu; JWEEMS@sjha.org Subject: RE: [Histonet] block alignment We currently are using the one marketed by Newcomer, which is the same one that has been previously mentioned as being designed by Mr. Davis in Colorado. They have two separate ones, one which will fit Leica and a different one which will fit Microm. They are not cheap, but it only has to save one specimen to be worth the money. Our techs didn't like them at first (felt they were a waste of money), but have grown to appreciate the importance. I don't have any experience with the Hiso Collimitor. Jennifer Fairview-University Medical Center Minneapolis, MN >>> "Bartlett, Jeanine" 03/03/05 08:14AM >>> The literature I have shows that it fits the majority of Microms. I know they are now the distributor but that hasn't always been the case. The one Newcomer has fits Microms and Leica, I believe. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 9:11 AM To: Bartlett, Jeanine; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment It only fits their microtomes. -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Thu 3/3/2005 8:48 AM To: Weems, Joyce; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Richard-Allan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, March 03, 2005 8:44 AM To: Dawson, Glen; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment From which company? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawson, Glen Sent: Thu 3/3/2005 8:27 AM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] block alignment Jeanine, I believe we have 7 Histo Collimators here and I've used one for at least 5 or 6 years and I couldn't go without it. Using the Collimator, I usually waste less than 10 microns worth of tissue on an outside block face-in. Thumbs Way Way Up on block aligners, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Bartlett, Jeanine [mailto:JQB7@CDC.GOV] Sent: Wednesday, March 02, 2005 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block alignment Hi: Does anyone out there have any experience with block alignment products such as the Microtome Aligner from Newcomer or the Histo Collimator? We receive a huge number of outside blocks and I adjust my block holder accordingly, but others in the group say it's easier to re-embed all outside blocks (Yikes!) and then have every microtome aligned the same. I was just wondering if these aligners work and if so perhaps it could be an alternative for those individuals that normally would rather re-embed than realign. Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. 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Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From vazquezr <@t> ohsu.edu Fri Mar 4 09:12:43 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] OCT embedding... Message-ID: Rebecca, Our researchers use it to hold their specimens to extract RNA from skin samples and seem to have no problems I believe. Robyn OHSU >>> "Rebecca Hands" 03/04/05 4:10 AM >>> Dear All, I am currently using OCT as an embedding medium for fresh frozen tissue. Ideally i need to cut sections from this embedded tissue, laser dissect specific areas of interest and extract high quality RNA from the lasered tissue for subsequent RT-PCR and microarray analysis. Does anyone know if OCT medium degrades RNA?...if so can RNase inhibitors be added to such solutions? Also does the actual embedding procedure itself comprise RNA integrity?...does anyone have a good protocol for this techniwque and subsequent good quality RNA extraction? Regards Rebecca Rebecca Hands (MSc) PhD Research Fellow Academic Dept of Surgery 4th Floor Alexandra Wing The Royal London Hospital Whitechapel Road Whitechapel London E1 1BB Ext 2614 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.A.Harper <@t> pcola.med.navy.mil Fri Mar 4 09:28:31 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Where Message-ID: <807FE48C5A7CC940B973B58D32E7014318A76762@nhpens-exch1.pcola.med.navy.mil> Where are the PA's schools? I'm all about corporate America...making more money. I just think histo techs don't get paid what they are worth if they are grossing. Thanks for all the opinions on grossing, now I'm going to learn to gross and am really tossing the PA school around. Does anybody go PT to PA school or do you have to do it FT? Have a great Friday histo world:-) Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola From TillRenee <@t> uams.edu Fri Mar 4 09:35:20 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] rat gi Message-ID: Does anyone have any suggestions for reducing background with rat gi? I have been trying to do a PCNA stain on distal and proximal colon with varying results. We use M0879 PC10 PCNA from Dako, which is a monoclonal mouse antibody, along with Biotin Conjugated Affinity Purified Goat anti-Mouse IgG from Rockland, and Peroxidase Conjugated Streptavidin from Jackson. I block with CAS block from Zymed and antigen retrieve with CitraPlus from Biogenex. I have also just tried using the Vectastain ABC Mouse kit, but that seems to turn out even worse than when I make my link and label. Could it be something as simple as longer washes or different antigen retrieval? Is it that important that you let the slides cool for the 20-30 mintues after retrieval as opposed to continuing right away or cooling them yourself? Or is gi just a tissue that is always going to give me background problems. Or could it be the antibody itself? I will admit I don't know enough about the specifics of how immunos work to figure out exactly what it is that could be causing the background staining. So far I have just tried to get them stained as best as possible without the background staining interfering with with my counting ( I have to count crypts cells by the intensity of staining, which is supposed to relate to the cell phase). Thanks, Renee' Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From petepath <@t> yahoo.com Fri Mar 4 09:50:28 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: <20050304155028.73251.qmail@web30409.mail.mud.yahoo.com> Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From liz <@t> premierlab.com Fri Mar 4 10:01:20 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] rat gi In-Reply-To: Message-ID: <000501c520d3$67cb4450$76d48a80@AMY> Till Try Dako's LSAB2 kit for rat specimens. I use it all the time for mouse monoclonal antibodies on rat specimens. With this kit it there is no background staining. I also use Dako's Serum free protein block. Off the histonet I'll send you some images of the same PCNA antibody on rat gut. I find with secondary reagents you need to check the specification sheet very carefully before purchasing, especially if you are dealing with animal tissue. On the spec sheet it will tell you the species crossreactivity. You need to check if your secondary will cross react with the same species of your tissue. I have bought secondary antibodies that I have in the fridge that I can't use (they were recommended by the vendor of the primary antibody) but they cross react with the tissue that I'm testing. I learned that little tid bit the hard way. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Friday, March 04, 2005 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rat gi Does anyone have any suggestions for reducing background with rat gi? I have been trying to do a PCNA stain on distal and proximal colon with varying results. We use M0879 PC10 PCNA from Dako, which is a monoclonal mouse antibody, along with Biotin Conjugated Affinity Purified Goat anti-Mouse IgG from Rockland, and Peroxidase Conjugated Streptavidin from Jackson. I block with CAS block from Zymed and antigen retrieve with CitraPlus from Biogenex. I have also just tried using the Vectastain ABC Mouse kit, but that seems to turn out even worse than when I make my link and label. Could it be something as simple as longer washes or different antigen retrieval? Is it that important that you let the slides cool for the 20-30 mintues after retrieval as opposed to continuing right away or cooling them yourself? Or is gi just a tissue that is always going to give me background problems. Or could it be the antibody itself? I will admit I don't know enough about the specifics of how immunos work to figure out exactly what it is that could be causing the background staining. So far I have just tried to get them stained as best as possible without the background staining interfering with with my counting ( I have to count crypts cells by the intensity of staining, which is supposed to relate to the cell phase). Thanks, Renee' Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joyce.christopher <@t> bayercropscience.com Fri Mar 4 10:01:35 2005 From: joyce.christopher <@t> bayercropscience.com (Joyce Christopher) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Klotz Solution Message-ID: Does anyone sell Klotz solution prepared? If not are there any suggestions/concerns for preparing? From plucas <@t> biopath.org Fri Mar 4 09:57:58 2005 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] EZ-Fix Message-ID: <000c01c520d2$f05cc600$7c01a8c0@biopath.org> Hello, Has anyone heard of or tried a product called EZ-Fix? A vendor told my administer of this, so I need to do some research on the product. It's described as a DMSO activated buffered alcoholic formalin that is used in the second and third station of the tissue processor. It is recommended for breast of other fatty tissues. It supposedly is 8x faster than formalin and 5x faster than Pen-Fix from Richard-Allan. Any discussions/comments are appreciated. Paula Bio-Path Medical Group From Rcartun <@t> harthosp.org Fri Mar 4 10:20:24 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Where Message-ID: To be perfectly honest, I think it requires more skill to cut a good paraffin section than it does to "gross" a biopsy specimen. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 03/04/05 10:28AM >>> Where are the PA's schools? I'm all about corporate America...making more money. I just think histo techs don't get paid what they are worth if they are grossing. Thanks for all the opinions on grossing, now I'm going to learn to gross and am really tossing the PA school around. Does anybody go PT to PA school or do you have to do it FT? Have a great Friday histo world:-) Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ynwang <@t> u.washington.edu Fri Mar 4 10:40:11 2005 From: ynwang <@t> u.washington.edu (Y. Wang) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] OCT embedding... In-Reply-To: <00f301c520b3$2a185640$b886258a@mds.qmw.ac.uk> References: <00f301c520b3$2a185640$b886258a@mds.qmw.ac.uk> Message-ID: Rebecca, We have recently aquired a laser capture microdissection system and have been embedding our tissue in OCT, capturing tissue for RT-PCR without much problem (although we are still quite new to this!). We just make sure that all our instruments that we use to take, embed and section the biopsy has been sterilized and washed in RNAse Away. Also we make sure that we embed as quickly as possible and store our blocs at -80deg. Our tissue is sectioned at -35 (we are working with skin) and sections are kept cold (store in -80) until use (we clean the cryostat out inbetween each bloc). We have an extremely fast staining protocol (I was surprised by how little time immuno could take!) that we adapted from Lindeman N et al. (Diagnostic Molecular pathology 2002 11(4):187-192) and we use a kit (picopure) for our RNA. So far we have had good results and it didn't take much time to set the procedures up. We have had good technical assistence from the LCM manufaturer (Arcturus) though. Check out the Arcturus web site if you need more technical info. Good luck Yak-Nam > Dear All, > > I am currently using OCT as an embedding medium for fresh frozen tissue. > Ideally i need to cut sections from this embedded tissue, laser dissect > specific areas of interest and extract high quality RNA from the lasered > tissue for subsequent RT-PCR and microarray analysis. Does anyone know > if OCT medium degrades RNA?...if so can RNase inhibitors be added to > such solutions? > > Also does the actual embedding procedure itself comprise RNA > integrity?...does anyone have a good protocol for this techniwque and > subsequent good quality RNA extraction? > > Regards > > Rebecca > > > Rebecca Hands (MSc) > > PhD Research Fellow > Academic Dept of Surgery > 4th Floor Alexandra Wing > The Royal London Hospital > Whitechapel Road > Whitechapel > London > E1 1BB > > Ext 2614 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Fri Mar 4 10:54:01 2005 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] cellsmears and cytospin for ihc Message-ID: Hi histonetters, We perform ihc on cellsmears and cytospins very rarely. Therefore we have no really experience. Can anyone tell me, if there is a general preperation protocol for these specimen, that is best for ihc? Air-drying / fixation in . / storage ? Or does it depend on the antibody or the kind of fluid? Thanks in advance Gudrun Lang Linz, Austria From mclarke <@t> allsaintshealthcare.org Fri Mar 4 11:14:49 2005 From: mclarke <@t> allsaintshealthcare.org (Clarke, Mary) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] toenails Message-ID: <3959B2255CA51443928A90517C973D6418EC4B@WFEXBE04.wfsi.priv> I am interested in finding out how other Histology labs deal with toenails. We get them and the pathologist wants them processed and stained for fungus. We have tried decal and that doesn't work very well. We have also tried Nair to soften them, but again that doesn't work real well either. Any help would be greatly appreciated. Thanks Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 From Charles.Embrey <@t> carle.com Fri Mar 4 11:26:00 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDDeltour <@t> mar.med.navy.mil Fri Mar 4 11:31:24 2005 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] toenails Message-ID: <080566D001A3D9459FFC0A391A646C9104BEF859@marxchg03.mar.med.navy.mil> Warm soapy water does the trick. -----Original Message----- From: Clarke, Mary [mailto:mclarke@allsaintshealthcare.org] Sent: Friday, March 04, 2005 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toenails I am interested in finding out how other Histology labs deal with toenails. We get them and the pathologist wants them processed and stained for fungus. We have tried decal and that doesn't work very well. We have also tried Nair to soften them, but again that doesn't work real well either. Any help would be greatly appreciated. Thanks Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Mar 4 11:38:04 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] toenails Message-ID: I suspect the success of procedures like soaking in Nair, Comfort, Mollifex, and even acid (I have given up trying to explain the illogic of this) is due to the one thing they have in common - soaking, i.e. water. My experience is that what I have called success, is limited in the extreme. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Deltour, Douglas D. (HM2) [mailto:DDDeltour@mar.med.navy.mil] Sent: 04 March 2005 17:31 To: 'Clarke, Mary'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] toenails Warm soapy water does the trick. -----Original Message----- From: Clarke, Mary [mailto:mclarke@allsaintshealthcare.org] Sent: Friday, March 04, 2005 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toenails I am interested in finding out how other Histology labs deal with toenails. We get them and the pathologist wants them processed and stained for fungus. We have tried decal and that doesn't work very well. We have also tried Nair to soften them, but again that doesn't work real well either. Any help would be greatly appreciated. Thanks Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Fri Mar 4 12:02:49 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] rat gi Message-ID: Renee, You could biotinylated your primary or you could try a rat adsorbed anti mouse secondary. I believe that Jackson has one made in horse. Sometimes this will decrease the sensitivity but that can usually be overcome with dilutions. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Till, Renee [mailto:TillRenee@uams.edu] Sent: Friday, March 04, 2005 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rat gi Does anyone have any suggestions for reducing background with rat gi? I have been trying to do a PCNA stain on distal and proximal colon with varying results. We use M0879 PC10 PCNA from Dako, which is a monoclonal mouse antibody, along with Biotin Conjugated Affinity Purified Goat anti-Mouse IgG from Rockland, and Peroxidase Conjugated Streptavidin from Jackson. I block with CAS block from Zymed and antigen retrieve with CitraPlus from Biogenex. I have also just tried using the Vectastain ABC Mouse kit, but that seems to turn out even worse than when I make my link and label. Could it be something as simple as longer washes or different antigen retrieval? Is it that important that you let the slides cool for the 20-30 mintues after retrieval as opposed to continuing right away or cooling them yourself? Or is gi just a tissue that is always going to give me background problems. Or could it be the antibody itself? I will admit I don't know enough about the specifics of how immunos work to figure out exactly what it is that could be causing the background staining. So far I have just tried to get them stained as best as possible without the background staining interfering with with my counting ( I have to count crypts cells by the intensity of staining, which is supposed to relate to the cell phase). Thanks, Renee' Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ekh9535 <@t> bjc.org Fri Mar 4 12:49:12 2005 From: ekh9535 <@t> bjc.org (Erin Herter) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] policy for slides for legal proc and sendouts Message-ID: Hi I am looking for someone willing to share their policies and procedures for sending out slides and or blocks for consultation and legal procedures. Thanks! Erin Erin Herter, Histology Department Alton Memorial Hospital One Memorial Drive Alton, IL 62002 618-463-7454 618-463-7641 fax ekh9535@bjc.org From Rcartun <@t> harthosp.org Fri Mar 4 13:44:00 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Fri Mar 4 14:18:37 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] toenails In-Reply-To: <3959B2255CA51443928A90517C973D6418EC4B@WFEXBE04.wfsi.priv> Message-ID: Terri, since we have cornered the "nail" market here in the southern part of Texas, we found out that soaking the block in warm soapy water works best also. We cut a couple of extras just in case the nail falls off the slide. We do a PAS/Fungus with a light green counter stain. The pathologists here love the contrast and staining so much that they stopped ordering a GMS on other cases. Joe "The Toe" Nocito Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Clarke, Mary Sent: Friday, March 04, 2005 11:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toenails I am interested in finding out how other Histology labs deal with toenails. We get them and the pathologist wants them processed and stained for fungus. We have tried decal and that doesn't work very well. We have also tried Nair to soften them, but again that doesn't work real well either. Any help would be greatly appreciated. Thanks Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Mar 4 17:39:34 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] EZ-Fix In-Reply-To: <000c01c520d2$f05cc600$7c01a8c0@biopath.org> References: <000c01c520d2$f05cc600$7c01a8c0@biopath.org> Message-ID: <4228F1B6.9060103@umdnj.edu> Hi Paul: Sales claims are just that, sales claims. Did your administrator expect the sales person to say EZ-fix was worse than Pen-Fix? And comparing an alcohol based fix to aqueous formalin is comparing apples to oranges. The only way you will know is to try it yourself. Geoff Paula Lucas wrote: >Hello, > >Has anyone heard of or tried a product called EZ-Fix? A vendor told my >administer of this, so I need to do some research on the product. > >It's described as a DMSO activated buffered alcoholic formalin that is used >in the second and third station of the tissue processor. It is recommended >for breast of other fatty tissues. It supposedly is 8x faster than formalin >and 5x faster than Pen-Fix from Richard-Allan. > >Any discussions/comments are appreciated. > >Paula >Bio-Path Medical Group > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From funderwood <@t> mcohio.org Fri Mar 4 14:36:57 2005 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] toenails Message-ID: Madge would recommend Palmolive. >>> "Joe Nocito" 03/04/05 03:18PM >>> Terri, since we have cornered the "nail" market here in the southern part of Texas, we found out that soaking the block in warm soapy water works best also. We cut a couple of extras just in case the nail falls off the slide. We do a PAS/Fungus with a light green counter stain. The pathologists here love the contrast and staining so much that they stopped ordering a GMS on other cases. Joe "The Toe" Nocito Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Clarke, Mary Sent: Friday, March 04, 2005 11:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toenails I am interested in finding out how other Histology labs deal with toenails. We get them and the pathologist wants them processed and stained for fungus. We have tried decal and that doesn't work very well. We have also tried Nair to soften them, but again that doesn't work real well either. Any help would be greatly appreciated. Thanks Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Mar 4 14:43:18 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: Some people don't like having the abbreviation Lab.Ass. after their name on their name tags. "Richard Cartun" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/04/2005 01:44 PM To: , , cc: Subject: RE: [Histonet] Pathology assistants programs What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Fri Mar 4 14:43:48 2005 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Sep 16 15:24:43 2005 Subject: FW: [Histonet] Pathology assistants programs Message-ID: I'll bet my salary against yours that you don't get paid like a lab assistant. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, March 04, 2005 2:44 PM To: Charles.Embrey@carle.com; histonet@lists.utsouthwestern.edu; petepath@yahoo.com Subject: RE: [Histonet] Pathology assistants programs What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ekh9535 <@t> bjc.org Fri Mar 4 14:53:42 2005 From: ekh9535 <@t> bjc.org (Erin Herter) Date: Fri Sep 16 15:24:43 2005 Subject: FW: [Histonet] Pathology assistants programs Message-ID: i'll take that action >>> "Charles, Roger" 3/4/2005 2:43:48 PM >>> I'll bet my salary against yours that you don't get paid like a lab assistant. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, March 04, 2005 2:44 PM To: Charles.Embrey@carle.com; histonet@lists.utsouthwestern.edu; petepath@yahoo.com Subject: RE: [Histonet] Pathology assistants programs What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vbaker60 <@t> yahoo.com Fri Mar 4 14:59:57 2005 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:24:43 2005 Subject: Fwd: RE: [Histonet] Pathology assistants programs Message-ID: <20050304205957.11856.qmail@web50105.mail.yahoo.com> To: Jackie M O'Connor Can you imagine "Path.Ass." after someone's name? Sorry, it's Friday afternoon and I'm getting punchy. --- Jackie M O'Connor > wrote: > > Some people don't like having the abbreviation > > Lab.Ass. after their name > > on their name tags. > > > > > > > > > > > > > > "Richard Cartun" > > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 03/04/2005 01:44 PM > > > > > > To: , > > , > > > > cc: > > Subject: RE: [Histonet] Pathology > > assistants programs > > > > > > What's wrong with being a lab assistant? I > consider > > myself a "lab > > assistant". > > > > Richard > > > > Richard W. Cartun, Ph.D. > > Director, Immunopathology & Histology > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 > > (860) 545-0174 Fax > > > > >>> "Charles.Embrey" > > 03/04/05 12:26PM >>> > > "Pathologists' Assistants" please. As a whole we > > don't like being > > called pathology assistants. It makes us sound > like > > lab assistants > > instead of assistants to the pathologist. If > anyone > > has questions > > about > > the program at Rosalind Franklin in Chicago, > please > > send me an e-mail > > or > > give me a call at (217)383-3666. > > > > Charles Embrey > > Pathologists' Assistant (AAPA) > > Urbana, IL > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] > > On Behalf Of > > Stephen > > Peters M.D. > > Sent: Friday, March 04, 2005 9:50 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Pathology assistants programs > > > > Heather, > > > > The AAPA web site contains all you need to find > out > > about this > > specialty. > > > > Here is a link with a listing of programs. > > > > > http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 > > > > > > Good Luck > > > > Stephen > > > > > > Stephen Peters M.D. > > Pathology Innovations, LLC > > 410 Old Mill Lane, > > Wyckoff, NJ 07481 > > 201 847 7600 > > www.pathologyinnovations.com > > > > Senior Attending Pathologist > > Hackensack University Medical Center > > 201 996 4836 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam > protection around > http://mail.yahoo.com > __________________________________ Celebrate Yahoo!'s 10th Birthday! Yahoo! Netrospective: 100 Moments of the Web http://birthday.yahoo.com/netrospective/ From kelly.mcqueeney <@t> bms.com Fri Mar 4 15:15:32 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:43 2005 Subject: FW: [Histonet] Pathology assistants programs In-Reply-To: References: Message-ID: <4228CFF4.6030903@bms.com> It's the same whether you work for a pathologist (pathologist's ass.) or scientist in a research lab...........still an assistant to someone. . Charles, Roger wrote: >I'll bet my salary against yours that you don't get paid like a lab >assistant. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard >Cartun >Sent: Friday, March 04, 2005 2:44 PM >To: Charles.Embrey@carle.com; histonet@lists.utsouthwestern.edu; >petepath@yahoo.com >Subject: RE: [Histonet] Pathology assistants programs > >What's wrong with being a lab assistant? I consider myself a "lab >assistant". > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > > >>>>"Charles.Embrey" 03/04/05 12:26PM >>> >>>> >>>> >"Pathologists' Assistants" please. As a whole we don't like being >called pathology assistants. It makes us sound like lab assistants >instead of assistants to the pathologist. If anyone has questions >about >the program at Rosalind Franklin in Chicago, please send me an e-mail >or >give me a call at (217)383-3666. > >Charles Embrey >Pathologists' Assistant (AAPA) >Urbana, IL > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Stephen >Peters M.D. >Sent: Friday, March 04, 2005 9:50 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Pathology assistants programs > >Heather, > >The AAPA web site contains all you need to find out about this >specialty. > >Here is a link with a listing of programs. > >http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 > >Good Luck > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From vazquezr <@t> ohsu.edu Fri Mar 4 15:32:25 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: Charles, Charles come down from your pedestal please. Robyn >>> "Richard Cartun" 03/04/05 11:44 AM >>> What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Fri Mar 4 16:09:43 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] IP 10 Message-ID: Is anyone doing any Immunohistochemistry using IP-10 any species frozen or paraffin? Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives From jkiernan <@t> uwo.ca Fri Mar 4 15:43:02 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] EZ-Fix Message-ID: <4228D666.64680035@uwo.ca> If this mixture is the 2nd or 3rd item on a tissue processor, what is the first? One of the functions of a fixative is to protect the tissue against adverse effects of processing. The fixative should be the first liquid a specimen encounters. Why would anyone want to use an alcoholic formaldehyde solution of unknown composition? There are plenty of published mixtures, mostly made with alcohol, formalin and acetic acid. All these liquids are inexpensive and are present in every lab that does histological work. There are also excellent published alcoholic fixatives that do not contain formaldehyde: Clarke's (3:1 ethanol-acetic) has been around for 154 years. Carnoy's (alcohol: chloroform:acetic, 60:30:10 ml) is about 115 years old. Methacarn (with MeOH instead of EtOH) is supposed to be better; I haven't used it myself, but am inclined to believe Holde Puchtler, who introduced methacarn. "Modified Carnoy" (James & Tas, 1984) is Carnoy with only 5 ml of acetic acid; it doesn't remove stainable RNA, which can happen with unduly long (overnight) exposure to regular Carnoy's. These alcohol-acetic fixatives can all give better micro-anatomical structural preservation better than is seen after neutral formaldehyde. Their reactions with proteins and nucleic acids are almost instantaneous, so fixation occurs with penetration - a few hours for ordinary specimens. These fixatives also do most of the dehydrating, and the specimens can be taken straight into the first 100% alcohol, saving time and materials. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Paula Lucas wrote: > > Hello, > > Has anyone heard of or tried a product called EZ-Fix? A vendor told my > administer of this, so I need to do some research on the product. > > It's described as a DMSO activated buffered alcoholic formalin that is used > in the second and third station of the tissue processor. It is recommended > for breast of other fatty tissues. It supposedly is 8x faster than formalin > and 5x faster than Pen-Fix from Richard-Allan. From maria <@t> ski.org Fri Mar 4 16:19:55 2005 From: maria <@t> ski.org (Maria Mejia) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] [Fwd: Re: c-fos] Message-ID: <4228DF0B.2080709@ski.org> -------- Original Message -------- Subject: Re: c-fos Date: Fri, 04 Mar 2005 14:12:05 -0800 From: Maria Mejia To: "LIAO,XIAOYAN" CC: histonet@lists.utsouthwester.edu References: <1109952713.422888c9657a4@mail.ucla.edu> Hello Liao, I would be happy to share my c-FOS protocol & I'm throwing in the ZIF-189 as a bonus. Use (agitiation) in steps from 5 to 15 especially the washing steps. Study of Immediate Early Response Genes using c-FOS & ZIF-189 Antibodies on Thick Free-Floating Section 1. Intracardiac perfusion of animal w/saline to wash vascular system followed by freshly made 4% paraformaldehyde/0.1M phosphate buffer (PB) at pH 7.4. 2. Store brain in 4% paraform. @ 4C - overnight. 3. Store brain in 30% sucrose/0.1M PB @ 4C - overnight (until brain sample sinks to bottom of container -can add 0.1-0.5% Thermosol (preservative) to sucrose solution to keep for longer period at 4C). 4. Cut brain using vibratome or sliding microtome section at 40 microns thick. Collect sections in wells containing freshly made 0.1M PB pH 7.4. The following is a special long term storing solution for immuno sections. Seal container with sections (well) and store @ 4C for several months: 30gm sucrose 1gm PVP-40 (1% polyvinylpyrolidone, Sigma #PVP40) 30ml ethylene glycol (30%) Make up the volume to 100ml w/0.1M PB pH 7.4. 5. Place tissue in soaking solution using gentle agitation @ room temperature - 2 hours. (until bubbles disappear - can use Tween 20 in place of TritionX - no bubbles). 8.7 ml Dulbecco's PBS (Sigma #D-5773) 300ul of 10% tritionX-100 1ml of 1% hydrogen peroxide (made from 30% H202) This solution makes about 10ml 6. Wash tissue well in 3 changes DPBS - 10-15 minutes each. 7. Place tissue in modified blocking solution for blocking nonspecific binding @ 4C w/gentle agitiation - overnight. 8.5ml of 2.5% BSA - I used Jackson ImmunoResearch. 300ul 10% Trition X-100 (again can use Tween-20) 1.5ml of super blocking solution (Innovex Bioscience) 100ul normal goat serum I picked up every third section and did c-FOS & ZIF-SC-189 alternatively: Before placing primary antibody on sections, leave a bit of the above blocking solution and using a pipette mix in with the antibody solution. 8. Place c-FOS (AB-5) Oncogene #PC38 Lot #D10535 primary polyclonal anti-rabbit and Santa Cruz ZIF (c-19) SC-189 Lot #B280 primary polyclonal anti-rabbit dilute both @ 1:10,000 with DPBS use gentle agitation @ room temp - 4 hrs. 9. Wash tissue well in DPBS X3 changes - 10-15 minutes each. 10. Place in secondary antibody biotinylated goat anti-rabbit (Vector BA-1000) dilute 1:1000/DPBS use gentle agitation @ room temp - 30 minutes. 11. Wash in DPBS X3 changes - 10-15 minutes each. 12. Place in Vectastain Elite ABC solution & incubate use gentle agitation at room temp - 30 minutes. 13. Wash well in DPBS X3 changes - 10-15 minutes each. 14. You can use any DAB substrate method with or without metals, e.g., nickel or cobalt. Mix desired DAB solution (agitate) and label - check under scope for intensity level. 15. Wash in DPBS X4 changes - 10-15 minutes each. 16. Mount on gelatin-coated glass slides. (I wash and coat my glass slides using a home- made gelatin protocol). 17. Air-dry slides - 1-2 days. 18. Dehydrate through x4 changes of absolute alcohol 100% - 10 minutes each. 19. Coverslip in DPX mounting media. I hope this protocol works as well as it did for me. regards Maria Bartola Mejia neurohistologist Smith-Kettlewell Eye Research Institute San Francisco, CA 94115 Email: maria@ski.org LIAO,XIAOYAN wrote: >hello, I am interested in your protocol for c-fos antibody staining in >thick free-floating sections. can you share with me your protocol and the >information about your antibody? Thanks. > > > > From Charles.Embrey <@t> carle.com Fri Mar 4 16:30:54 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: Robyn, You speak like you know me...don't. If you did know me you would understand that I am not speaking from "my pedestal" or down to anyone. I started life in the lab 26 years ago as a young Airman fresh out of high school. I worked as a histotech for 20 years and went on to my pathologists' assistant fellowship with the AAPA. I have gladly offered help and mentorship to anyone that asked at anytime. Actually I am a very light hearted and easy going guy that just wanted to clarify the job title. Sorry if I offended you or anyone else on Histonet in any way. ASCP has now listed the certification as Pathologists' Assistant and the initials as PA(ASCP). Charles Embrey Pathologists' Assistant Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Friday, March 04, 2005 3:32 PM To: Charles.Embrey; Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu; petepath@yahoo.com Subject: RE: [Histonet] Pathology assistants programs Charles, Charles come down from your pedestal please. Robyn >>> "Richard Cartun" 03/04/05 11:44 AM >>> What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TheBestTime23 <@t> aol.com Fri Mar 4 17:28:00 2005 From: TheBestTime23 <@t> aol.com (TheBestTime23@aol.com) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] tried posting this once already... didn't work. Message-ID: <1e1.374ee912.2f5a4900@aol.com> OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech From haldana <@t> unimoron.edu.ar Fri Mar 4 18:13:58 2005 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] The best cutting angle Message-ID: <00a001c52118$3a5c8200$a904a8c0@um.edu> Dears What is the best cutting angle on the microtome for paraffins sectioning using disposable blades. I have a Microm microtome that shows 0 to 17 variable angle in the blade holder. Thanks in advance Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html From LuckG <@t> empirehealth.org Fri Mar 4 18:27:40 2005 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: Richard, You're on a roll today! That's two for two correct for you (i.e. your comment on microtomy compared to grossing earlier) and your response to the offense taken at the pathology assistant label. Bravo, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Friday, March 04, 2005 11:44 AM To: Charles.Embrey@carle.com; histonet@lists.utsouthwestern.edu; petepath@yahoo.com Subject: RE: [Histonet] Pathology assistants programs What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From msdfloyd <@t> worldnet.att.net Sat Mar 5 07:55:07 2005 From: msdfloyd <@t> worldnet.att.net (Dina Floyd) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] The best cutting angle References: <00a001c52118$3a5c8200$a904a8c0@um.edu> Message-ID: <000801c5218a$f5729da0$e77e410c@yourxhtr8hvc4p> We set ours at 10, put that is personal preference. ----- Original Message ----- From: "Hernan Aldana Marcos" To: <> Sent: Friday, March 04, 2005 6:13 PM Subject: [Histonet] The best cutting angle Dears What is the best cutting angle on the microtome for paraffins sectioning using disposable blades. I have a Microm microtome that shows 0 to 17 variable angle in the blade holder. Thanks in advance Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From p_bourne_14526 <@t> yahoo.com Sat Mar 5 08:00:30 2005 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs In-Reply-To: Message-ID: <20050305140030.59858.qmail@web53708.mail.yahoo.com> The more years I live it always amazes me how we humans feel the need to separate ourselves from one another. Pathologist, Pathologist Assistant, Histotechnologist, Histotechician, Lab Assistant, Secretary, Enviromental Engineer etc.does it really matter? Without each other we could not function so in some ways we are all assistants to each other. We are a team. Over these past 30 years, I have known the good, the bad, and the ugly under each title so your title has nothing to do with your abilities. The pay differential should be enough for those who feel the need to separate but it should stop at the bank. We are all in this together. We all have a job to complete. We all should be willing to extend a helping hand to one another and treat each other with the respect which everyone deserves no matter what the title. So let's just put the titles away and get the job done without all this nonsense. --------------------------------- Celebrate Yahoo!'s 10th Birthday! Yahoo! Netrospective: 100 Moments of the Web From Rachael_Emerson <@t> URMC.Rochester.edu Sat Mar 5 12:53:51 2005 From: Rachael_Emerson <@t> URMC.Rochester.edu (Emerson, Rachael) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] C. elegans fixation Message-ID: Hello. Does anyone have protocols or suggestions on fixing the embryological stage of C.elegans onto a microscope slide? I have a colleague who is working with these animals and trying to perform IHC. Thank you Rachael Emerson From rsukumar <@t> dpspa.com Sun Mar 6 14:27:45 2005 From: rsukumar <@t> dpspa.com (Ray Sukumar) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 12 In-Reply-To: <20050306185412.64F4534069@cmlapp23.siteprotect.com> Message-ID: How do you find a list of 'slide-prep' labs for us to use if there is an emergency? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, March 06, 2005 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. C. elegans fixation (Emerson, Rachael) ---------------------------------------------------------------------- Message: 1 Date: Sat, 5 Mar 2005 13:53:51 -0500 From: "Emerson, Rachael" Subject: [Histonet] C. elegans fixation To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello. Does anyone have protocols or suggestions on fixing the embryological stage of C.elegans onto a microscope slide? I have a colleague who is working with these animals and trying to perform IHC. Thank you Rachael Emerson ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 12 **************************************** From JMacDonald <@t> mtsac.edu Sun Mar 6 16:12:47 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] ASCP, schools and education Message-ID: You guessed wrong on the availability of schools for Histotechnol ogy. If you check the NSH website you can see the schools and hospita These are N Associate Degrees. the minimum educa Jennifer MacD Mt. San Antonio College (one of the 12 schools offering as Degree) -----histonet-bounces@list To: histonet@lists.utsouthwestern.edu From: T Sent by: histonet-bounces@lists.utsouthwestern.edu< 03/04/2005 03:28PM Subject: [Histonet] tried posting this once work. OK. I and was really surprised by what I got. I want to start by apologizing to al have offended. It surely wasn't my intent. A thought of a lot of things to say in response to keep this within reason. < to have gotten i that pays me well, keeps me interested and has a lot for growth. I take pride in the work that I do every day. I have recently couldn't be more Second, and probably more to the point: I was really tr make a comment about the requirements for being a histote statement about the job itself. The e-mail I was of college as a pre-req, and probably have a s one sem but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histot had 4 years of college as a requirement and ad stuff, you're looking at 6 years. that amount of ti still be working in a similar field. That was my only real point. I &n want people to have more education and that's way that the ASCP also takes credit hours looking for a degree. Bu I would < would not have requirements been that stiff 20 years ago. I guess my th to be a h i started as a lab aid, heard about on the job traini from there. I know a lot of people who started something similar. How man know that most people get a totally blank look on their face when you tell them th work in histology. I had certainly never heard of it How many of you had? I can't see many people looki saying to themselves, "oh, histology because most of them wouldn't kn is mostly a guess) there aren't any 2 year programs at tech schools or anyth ing like that. Histology is kind of an anomaly that way. Ta and clinics, but not schools. Maybe the on &nbs a bad thing. At least it would get more concrete metho thought. hope won't get me into any more trouble : ) < Megan Grateful new histotech ____ ______________________ 5F__ Histonet mailing Histonet@lists.utsouthwestern.edu [1]http://lists.utsou References 1. 3D"http://lists.utsout=/ From joyce.christopher <@t> bayercropscience.com Mon Mar 7 06:56:45 2005 From: joyce.christopher <@t> bayercropscience.com (Joyce Christopher) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Klotz Message-ID: Is Klotz available for purchase anywhere? Are there any suggestions or concerns for preparation should I have to prepare ? From kelly.mcqueeney <@t> bms.com Mon Mar 7 07:52:56 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] The best cutting angle In-Reply-To: <00a001c52118$3a5c8200$a904a8c0@um.edu> References: <00a001c52118$3a5c8200$a904a8c0@um.edu> Message-ID: <422C5CB8.4060805@bms.com> I like 10 degrees, it always works for me. Good luck, Kelly Hernan Aldana Marcos wrote: >Dears >What is the best cutting angle on the microtome for paraffins sectioning using disposable blades. I have a Microm microtome that shows 0 to 17 variable angle in the blade holder. >Thanks in advance > >Dr. Hern?n J. Aldana Marcos >Facultad de Medicina. Universidad de Mor?n >Machado 914. B1708JPD. Buenos Aires. Argentina >e-mail alternativo hernanjavier@yahoo.com >web: http://hjaldanamarcos.bravepages.com >http://histologia.bigthicketdirectory.net/main.html > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JNocito <@t> Pathreflab.com Mon Mar 7 08:04:10 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs In-Reply-To: Message-ID: Hey Chuck, here's some water to douse those flames Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Friday, March 04, 2005 4:31 PM To: Robyn Vazquez Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathology assistants programs Robyn, You speak like you know me...don't. If you did know me you would understand that I am not speaking from "my pedestal" or down to anyone. I started life in the lab 26 years ago as a young Airman fresh out of high school. I worked as a histotech for 20 years and went on to my pathologists' assistant fellowship with the AAPA. I have gladly offered help and mentorship to anyone that asked at anytime. Actually I am a very light hearted and easy going guy that just wanted to clarify the job title. Sorry if I offended you or anyone else on Histonet in any way. ASCP has now listed the certification as Pathologists' Assistant and the initials as PA(ASCP). Charles Embrey Pathologists' Assistant Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Friday, March 04, 2005 3:32 PM To: Charles.Embrey; Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu; petepath@yahoo.com Subject: RE: [Histonet] Pathology assistants programs Charles, Charles come down from your pedestal please. Robyn >>> "Richard Cartun" 03/04/05 11:44 AM >>> What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From xia <@t> oakland.edu Mon Mar 7 08:16:53 2005 From: xia <@t> oakland.edu (xia@oakland.edu) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Postdoctoral Position in Cartilage Research Message-ID: We have been studying articular cartilage using multidisciplinary imaging techniques, including non-invasive microscopic magnetic resonance imaging (?MRI), polarized light microscopy (PLM), transmission electron microscopy (TEM), Fourier-transform infrared imaging (FTIRI), tissue histology, biochemical treatment, and mechanical characterization. The results from our fundamental research at microscopic resolution are used to guide the successful applications of non-invasive procedures in clinical management of osteoarthritis and related joint diseases. The successful candidate for this postdoctoral position should have a PhD or equivalent in biomedical sciences, tissue histology, bioengineering, or a related field. A solid background and previous knowledge in cartilage or other connective tissue is required. Working experience and skills with modern instrument and computer will be an asset. This is an excellent opportunity for a highly motivated individual with solid biological/medical background who wants to participate in the multidisciplinary research activities. Oakland University is located in suburban Rochester, Michigan, in north Oakland County, which boasts one of the most picturesque campuses in the country. Our state-of-art instrumentation includes a ?MRI system (Bruker AMX300 with a 7T wide-bore superconducting magnet and microimaging accessories), a mechanical testing system (EnduraTec ELF 3200), a digital polarized light microscope (Leica DM RXP interfaced with a 12-bit CCD camera), a soon-to-be-installed FTIRI system, and a number of histology equipments. In addition, research times on a Bruker 7T/20cm MRI system and a GE 3T whole-body MRI system are available at a nearby institution. Our web site contains more information regarding our lab and some of the recently completed cartilage projects. Interested individuals should send their CV, statements of research experience and research interest, and the names, telephone numbers, and e-mail addresses of at least three references to: Dr. Yang Xia Associate Professor of Physics Dept of Physics, Oakland University, Rochester, MI 48309, USA Tel: 248-370-3420; Fax: 248-370-3408; E-mail: xia@oakland.edu Web: http://www.oakland.edu/~xia/XiaLab_index.html. OU is an equal opportunity employer. -- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Yang Xia, Ph. D. 276 Hannah Hall, Physics Department Oakland University, Rochester, MI 48309-4487, USA Tel: (248) 370-3420(office), 370-2403(NMR), 370-3402 & 370-4873 (labs) Fax: (248) 370-3408 Email: xia@oakland.edu Web: http://www.oakland.edu/~xia/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From Kemlo.Rogerson <@t> elht.nhs.uk Mon Mar 7 08:46:58 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] The best cutting angle[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F061@bhrv-nt-11.bhrv.nwest.nhs.uk> If I remember, from the dim distant past, you could alter the angle for different types of tissue; but I can't remember why. Don't knives with narrower profiles have to be at a shallower angle to prevent 'chatters' or is that the 'old' type knives that I bear the scars from on my fingers? Used to sharpen them with wooden sticks and diamond paste; you slipped now and again, but A&E were very helpful in sewing bits back on, except for the end of my thumb. Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Kelly D Mcqueeney [mailto:kelly.mcqueeney@bms.com] Sent: 07 March 2005 13:53 To: Hernan Aldana Marcos Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The best cutting angle[Scanned] I like 10 degrees, it always works for me. Good luck, Kelly Hernan Aldana Marcos wrote: >Dears >What is the best cutting angle on the microtome for paraffins sectioning using disposable blades. I have a Microm microtome that shows 0 to 17 variable angle in the blade holder. >Thanks in advance > >Dr. Hern?n J. Aldana Marcos >Facultad de Medicina. Universidad de Mor?n >Machado 914. B1708JPD. Buenos Aires. Argentina >e-mail alternativo hernanjavier@yahoo.com >web: http://hjaldanamarcos.bravepages.com >http://histologia.bigthicketdirectory.net/main.html > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From julien_lambreydesouza <@t> uqar.qc.ca Mon Mar 7 08:47:47 2005 From: julien_lambreydesouza <@t> uqar.qc.ca (Julien LambreyDeSouza) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] overprocessed plant tissue? Message-ID: <6.1.1.1.1.20050307082706.0196fcf0@pop3.uqar.qc.ca> Hello, I am trying to cut embedded white Birch embryos. Because the seedcoat (mainly scl?renchyme) is too hard, we take the embryos out of the seedcoat and process them. I think, we're getting overprocessed (fried?) embryos, but then again, I am unexperienced with plant tissues. What would I have to look for in order to determine overprocessed tissue? The cells in the embryo are clearly visible, but their contents seems to have binded into spherical masses, like small pink marbles (colored in eosine). The nucleus sometimes appears blue (heamatox) in between the masses. The processing goes as follows: EtOH 70% 30min EtOH 80% 30min EtOH 90% 30min EtOH100% 30min EtOH100% 30min EtOH100% 30min EtOH100% 30min EtOH100% + Xylene (50/50) 30min Xylene 30min Xylene 45min Paraffin 45 min Paraffin 1H30 Coloration: Xylene 3min Xylene 3min Xylene 3min EtOH 100% 2min EtOH 100% 2min EtOH 100% 3min EtOH 95% 2min EtOH 95% 2min EtOH 70% 2min Water 2min Hematoxylin 4min Water 2min Bluing reagent 2min water 3min EtOH 95% 30sec Eosine 2min EtOH 95% 1min EtOH 95% 1min EtOH 100% 1min EtOH 100% 2min EtOH 100% 3min Xylene 3min Xylene 3min I posted some pics to the histonet site this morning. They should be their in 24H. Look for "plant embryo-10x.jpg", "plant embryo-40x.jpg" (3 pics) and "plant embryo-100x.jpg". In the meantime, does anybody have any suggestion to what the problem may be? Thanks, Julien Lambrey de Souza Biologie ?volutive, Universit? du Qu?bec ? Rimouski (418) 723-1986 #1714 From BlazekL <@t> childrensdayton.org Mon Mar 7 08:54:38 2005 From: BlazekL <@t> childrensdayton.org (Linda Blazek) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: Hey Chuck, maybe Robyn doesn't know you but there are people here that really do know you and we are trying to get a consensus of opinion on that one. "Actually I am a very light hearted and easy going guy" But we all agree that your incite and information that you share has always been appreciated. (With the exception of your attempt to recruit out supervisor) Linda Blazek, HT (ASCP) Department of Pathology Children's Medical Center Dayton, Ohio 45404 (937) 641-3358 fax (937)641-5482 blazekl@childrensdayton.org >>> "Charles.Embrey" 03/04/2005 5:30:54 PM >>> Robyn, You speak like you know me...don't. If you did know me you would understand that I am not speaking from "my pedestal" or down to anyone. I started life in the lab 26 years ago as a young Airman fresh out of high school. I worked as a histotech for 20 years and went on to my pathologists' assistant fellowship with the AAPA. I have gladly offered help and mentorship to anyone that asked at anytime. Actually I am a very light hearted and easy going guy that just wanted to clarify the job title. Sorry if I offended you or anyone else on Histonet in any way. ASCP has now listed the certification as Pathologists' Assistant and the initials as PA(ASCP). Charles Embrey Pathologists' Assistant Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Friday, March 04, 2005 3:32 PM To: Charles.Embrey; Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu; petepath@yahoo.com Subject: RE: [Histonet] Pathology assistants programs Charles, Charles come down from your pedestal please. Robyn >>> "Richard Cartun" 03/04/05 11:44 AM >>> What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Mon Mar 7 09:10:26 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: So Matt has been talking.... It's only a matter of time Linda. I'll talk him into the move. We can be histology's "children of the corn". Chuck -----Original Message----- From: Linda Blazek [mailto:BlazekL@childrensdayton.org] Sent: Monday, March 07, 2005 8:55 AM To: Charles.Embrey; vazquezr@ohsu.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathology assistants programs Hey Chuck, maybe Robyn doesn't know you but there are people here that really do know you and we are trying to get a consensus of opinion on that one. "Actually I am a very light hearted and easy going guy" But we all agree that your incite and information that you share has always been appreciated. (With the exception of your attempt to recruit out supervisor) Linda Blazek, HT (ASCP) Department of Pathology Children's Medical Center Dayton, Ohio 45404 (937) 641-3358 fax (937)641-5482 blazekl@childrensdayton.org >>> "Charles.Embrey" 03/04/2005 5:30:54 PM >>> Robyn, You speak like you know me...don't. If you did know me you would understand that I am not speaking from "my pedestal" or down to anyone. I started life in the lab 26 years ago as a young Airman fresh out of high school. I worked as a histotech for 20 years and went on to my pathologists' assistant fellowship with the AAPA. I have gladly offered help and mentorship to anyone that asked at anytime. Actually I am a very light hearted and easy going guy that just wanted to clarify the job title. Sorry if I offended you or anyone else on Histonet in any way. ASCP has now listed the certification as Pathologists' Assistant and the initials as PA(ASCP). Charles Embrey Pathologists' Assistant Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Friday, March 04, 2005 3:32 PM To: Charles.Embrey; Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu; petepath@yahoo.com Subject: RE: [Histonet] Pathology assistants programs Charles, Charles come down from your pedestal please. Robyn >>> "Richard Cartun" 03/04/05 11:44 AM >>> What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Mon Mar 7 09:13:30 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] tried posting this once already... didn't work. Message-ID: BRAVO Megan!!!!!!!!!!!!!!!!!!!!!!! I to fell into being a histotech. ALL started in the military. I was trained as a lab tech, but a billet came open for a lab tech in a histotechs job. Out of military into guess what? A histo job. All I had was On the job training, no schooling, just self taught and a lot of patient senior techs. Wahla, 14 years later and still a histotech without formal schooling for this position. My docs expect a great deal from me and I deliver without four years of schooling. Thanks for letting me share my views. Robyn OHSU >>> 03/04/05 3:28 PM >>> OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Mon Mar 7 09:16:57 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Pathology assistants programs Message-ID: Charles, No I don't know you and I am sure I could learn a lot from you. I think it is great that you went on and furthered your education, but not all people want to. Robyn OHSU >>> "Charles.Embrey" 03/04/05 2:30 PM >>> Robyn, You speak like you know me...don't. If you did know me you would understand that I am not speaking from "my pedestal" or down to anyone. I started life in the lab 26 years ago as a young Airman fresh out of high school. I worked as a histotech for 20 years and went on to my pathologists' assistant fellowship with the AAPA. I have gladly offered help and mentorship to anyone that asked at anytime. Actually I am a very light hearted and easy going guy that just wanted to clarify the job title. Sorry if I offended you or anyone else on Histonet in any way. ASCP has now listed the certification as Pathologists' Assistant and the initials as PA(ASCP). Charles Embrey Pathologists' Assistant Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Friday, March 04, 2005 3:32 PM To: Charles.Embrey; Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu; petepath@yahoo.com Subject: RE: [Histonet] Pathology assistants programs Charles, Charles come down from your pedestal please. Robyn >>> "Richard Cartun" 03/04/05 11:44 AM >>> What's wrong with being a lab assistant? I consider myself a "lab assistant". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Charles.Embrey" 03/04/05 12:26PM >>> "Pathologists' Assistants" please. As a whole we don't like being called pathology assistants. It makes us sound like lab assistants instead of assistants to the pathologist. If anyone has questions about the program at Rosalind Franklin in Chicago, please send me an e-mail or give me a call at (217)383-3666. Charles Embrey Pathologists' Assistant (AAPA) Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: Friday, March 04, 2005 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology assistants programs Heather, The AAPA web site contains all you need to find out about this specialty. Here is a link with a listing of programs. http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Good Luck Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joyce.christopher <@t> bayercropscience.com Mon Mar 7 09:53:27 2005 From: joyce.christopher <@t> bayercropscience.com (Joyce Christopher) Date: Fri Sep 16 15:24:43 2005 Subject: [Histonet] Klotz Message-ID: Hope this not a repeat. Looking to purchase prepared Klotz Solution. Any companies which provide? Are there any concerns or suggestions in preparation should I have to prepare? From gcallis <@t> montana.edu Mon Mar 7 09:54:28 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Re:The best cutting angle In-Reply-To: <000801c5218a$f5729da0$e77e410c@yourxhtr8hvc4p> References: <00a001c52118$3a5c8200$a904a8c0@um.edu> <000801c5218a$f5729da0$e77e410c@yourxhtr8hvc4p> Message-ID: <6.0.0.22.1.20050307084624.01b664a8@gemini.msu.montana.edu> A Richard Allen MICROM microtomy specialist gave hints that Richard Allen Edge Rite blades high profile are approx 11 degrees, and low profile may need to be a bit more at 12 degrees. However, blades from different manufacturers may have differences, although small and if one has difficulty with angles using any given blade, start at around 11 degrees or so (10 degrees would work too) and increase or decrease the angle in very tiny increments until you have optimal cutting for the blades one purchased. Differents lots of blades from any one manufacturer can have differences too, so learning to adjust angles is critical for good microtomy. At 06:55 AM 3/5/2005, you wrote: We set ours at 10, put that is personal preference. >----- Original Message ----- From: "Hernan Aldana Marcos" > >Subject: [Histonet] The best cutting angle > >What is the best cutting angle on the microtome for paraffins sectioning >using disposable blades. I have a Microm microtome that shows 0 to 17 >variable angle in the blade holder. >Thanks in advance Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From sbreeden <@t> nmda.nmsu.edu Mon Mar 7 10:13:09 2005 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] NexEs Protocol for Canine Distemper Virus Message-ID: I'm looking for a protocol for CDV to be run on the Ventana NexEs. The only protocol I have is one for a Dako and I'm new enough to this that I'm not sure of the conversion parameters from one manufacturer to another. I'd appreciate any help... Sara A. Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From kosmicdog <@t> hotmail.com Mon Mar 7 10:27:16 2005 From: kosmicdog <@t> hotmail.com (J m) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] IHC humidity chamber? Message-ID: salut, Does anyone know of a supplier in Canada for the IHC humidity chambers. I used to have a black plastic one with a clear lid but I don't know where it came from or where i can get another one. I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Regular tissue sections seem to "hold" the solution on the slide better than tissue arrays which are more apt to losing there solutions. I don't like using water repelent pens to circle the arrays but any suggestions would be appreciated. From mcclistd <@t> musc.edu Mon Mar 7 10:29:38 2005 From: mcclistd <@t> musc.edu (David McClister) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] mouse heart morphology Message-ID: Hello, I am embedding longitudinal sectioned mice heart in OTC and need to have better morphology to be able to clearly see both left and right ventricles. This lab has tried dentamold but I found it very difficult to section. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks David McClister, HTL (ASCP) Medical University of South Carolina Dept of Cardiothoracic Research 117 Doughty St Charleston, SC 29425 From kelly.mcqueeney <@t> bms.com Mon Mar 7 11:05:59 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] [Fwd: Re: c-fos] In-Reply-To: <4228DF0B.2080709@ski.org> References: <4228DF0B.2080709@ski.org> Message-ID: <422C89F7.8020609@bms.com> Hi Maria, Have you tried c-fos IHC on brain mounted on slides rather than processed in a plate? Thanks, Kelly Maria Mejia wrote: > > > -------- Original Message -------- > Subject: Re: c-fos > Date: Fri, 04 Mar 2005 14:12:05 -0800 > From: Maria Mejia > To: "LIAO,XIAOYAN" > CC: histonet@lists.utsouthwester.edu > References: <1109952713.422888c9657a4@mail.ucla.edu> > > > > Hello Liao, I would be happy to share my c-FOS protocol & I'm throwing > in the > ZIF-189 as a bonus. Use (agitiation) in steps from 5 to 15 especially > the washing > steps. > > Study of Immediate Early Response Genes using c-FOS & ZIF-189 > Antibodies on > Thick Free-Floating Section > 1. Intracardiac perfusion of animal w/saline to wash vascular system > followed by freshly > made 4% paraformaldehyde/0.1M phosphate buffer (PB) at pH 7.4. > 2. Store brain in 4% paraform. @ 4C - overnight. > 3. Store brain in 30% sucrose/0.1M PB @ 4C - overnight (until brain > sample sinks to > bottom of container -can add 0.1-0.5% Thermosol (preservative) to > sucrose solution to > keep for longer period at 4C). > 4. Cut brain using vibratome or sliding microtome section at 40 > microns thick. Collect > sections in wells containing freshly made 0.1M PB pH 7.4. > The following is a special long term storing solution for immuno > sections. Seal container > with sections (well) and store @ 4C for several months: > 30gm sucrose > 1gm PVP-40 (1% polyvinylpyrolidone, Sigma #PVP40) > 30ml ethylene glycol (30%) > Make up the volume to 100ml w/0.1M PB pH 7.4. > > 5. Place tissue in soaking solution using gentle agitation @ room > temperature - 2 hours. > (until bubbles disappear - can use Tween 20 in place of TritionX - no > bubbles). > 8.7 ml Dulbecco's PBS (Sigma #D-5773) > 300ul of 10% tritionX-100 > 1ml of 1% hydrogen peroxide (made from 30% H202) > This solution makes about 10ml > 6. Wash tissue well in 3 changes DPBS - 10-15 minutes each. > 7. Place tissue in modified blocking solution for blocking > nonspecific binding @ 4C > w/gentle agitiation - overnight. > 8.5ml of 2.5% BSA - I used Jackson ImmunoResearch. > 300ul 10% Trition X-100 (again can use Tween-20) > 1.5ml of super blocking solution (Innovex Bioscience) > 100ul normal goat serum > > I picked up every third section and did c-FOS & ZIF-SC-189 alternatively: > Before placing primary antibody on sections, leave a bit of the above > blocking solution > and using a pipette mix in with the antibody solution. > > 8. Place c-FOS (AB-5) Oncogene #PC38 Lot #D10535 primary polyclonal > anti-rabbit > and Santa Cruz ZIF (c-19) SC-189 Lot #B280 primary polyclonal > anti-rabbit dilute both > @ 1:10,000 with DPBS use gentle agitation @ room temp - 4 hrs. > 9. Wash tissue well in DPBS X3 changes - 10-15 minutes each. > 10. Place in secondary antibody biotinylated goat anti-rabbit (Vector > BA-1000) dilute > 1:1000/DPBS use gentle agitation @ room temp - 30 minutes. > 11. Wash in DPBS X3 changes - 10-15 minutes each. > 12. Place in Vectastain Elite ABC solution & incubate use gentle > agitation at room > temp - 30 minutes. > 13. Wash well in DPBS X3 changes - 10-15 minutes each. > 14. You can use any DAB substrate method with or without metals, > e.g., nickel or cobalt. > Mix desired DAB solution (agitate) and label - check under scope for > intensity level. > 15. Wash in DPBS X4 changes - 10-15 minutes each. > 16. Mount on gelatin-coated glass slides. (I wash and coat my glass > slides using a home- > made gelatin protocol). > 17. Air-dry slides - 1-2 days. > 18. Dehydrate through x4 changes of absolute alcohol 100% - 10 > minutes each. > 19. Coverslip in DPX mounting media. > > I hope this protocol works as well as it did for me. > > regards > Maria Bartola Mejia > neurohistologist > Smith-Kettlewell Eye Research Institute > San Francisco, CA 94115 > Email: maria@ski.org > > > > > > > > > > > > > > > > > > > > > > > > > > > > LIAO,XIAOYAN wrote: > >> hello, I am interested in your protocol for c-fos antibody staining in >> thick free-floating sections. can you share with me your protocol and >> the >> information about your antibody? Thanks. >> >> >> >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Mon Mar 7 11:17:55 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] mouse heart morphology Message-ID: <20050307171755.7289.qmail@web30410.mail.mud.yahoo.com> Dear David, I have developed a system for embedding frozen sections that will allow you to section your mouse hearts any way you desire. The embedding is done face down in freezing temperature well bars. If you visit my web site you will see a display of many techniques possible with this simple apparatus. I am also just introducing with a larger well bar (30 x 45 mm wells )and chucks which will allow you to embed a whole mouse. Please feel free to call me if you have any questions. http://pathologyinnovations.com/index.html Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From gcallis <@t> montana.edu Mon Mar 7 11:38:23 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] mouse heart morphology In-Reply-To: <20050307171755.7289.qmail@web30410.mail.mud.yahoo.com> References: <20050307171755.7289.qmail@web30410.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20050307103352.01b1fcf8@gemini.msu.montana.edu> Are you freezing inside the cryostat or using a solvent/liquid nitrogen method, or dry ice/isopentane snap freezing? When we have frozen mouse heart, for that matter, any murine tissues inside a cryostat using Peltier device cooling platforms, we get horrible freezing artifact with terrible morphology. Murine tissue snap freezing tends to be more stringent, and unfortunately the damage caused by slower, lower temperture inside cryostat freezing method. Can you use your device with liquid nitrogen surrounding it? If so, then murine tissue morphology would be greatly improved. At 10:17 AM 3/7/2005, you wrote: >Dear David, > >I have developed a system for embedding frozen sections that will allow >you to section >your mouse hearts any way you desire. The embedding is done face down in > freezing temperature well bars. If you visit my web site you will see a > display of > many techniques possible with this simple apparatus. I am also just > introducing with a >larger well bar (30 x 45 mm wells )and chucks which will allow you to >embed a whole mouse. >Please feel free to call me if you have any questions. > >http://pathologyinnovations.com/index.html > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon Mar 7 12:25:04 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] mouse heart morphology In-Reply-To: <20050307175232.56062.qmail@web30402.mail.mud.yahoo.com> References: <20050307175232.56062.qmail@web30402.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20050307105927.01b45ab8@gemini.msu.montana.edu> Stephen, Sorry buy -40C is NOT cold enough for freezing our murine tissues. I believe that others have experienced this problem also, the one of getting freezing artifact/damaged morphology with inside cryostat temperature freezing. Nothing scary about liquid nitrogen usage, although Isopentane is more of a worry for storage. I was thinking more of precooling the metal device containing wells i.e surround the device with liquid nitrogen, then you have the wells at the extremely cold temperatures. Liquid nitrogen in the wells would not be ideal, to be sure. We do this with a metal block sitting in liquid nitrogen so the metal block is at liquid nitrogen temperatures, then place an OCT embedded murine tissue (inside a Tissue Tek plastic mold) on top of the very cold metal block. This permits murine tissue to freeze at a much colder temperature and without artifact. I have seen your device and like its design, but if mouse tissue cannot be snap frozen at colder temperature than -40C, then we can't use it due to terrible morphology. Cryostat freezing temperatures for murine cryomicrotomy simply do not do the job well enough. It is the water ice crystal formation inside the tissue that presents the big problem and the colder the freezing temperature, the smaller the ice crystal formation - hence snap freezing in the true sense of the words. Precooling OCT and a tissue is not feasible when you have 10 mice lined up and collecting 15 tissues out of each mouse as fast as you can dissect and snap freeze plus the actual snap freezing takes only seconds or so - extremely fast. I would love to try your device in the way we use our metal block surround by liquid nitrogen as your device has a very efficient design. There is no doubt it works very well for clinical laboratories for rapid diagnostic work, but for our fussy murine tissues, colder temperatures must prevail. There is an excellent discussion by Charles Scouten on snap freezing biological samples can be found at www.myneurolab.com with details on ice crystal formation, etc. - what we experience with murine tissue. Gayle Callis At 10:52 AM 3/7/2005, you wrote: >HI Gail, > >I have not experimented with liquid nitrogen in my wells. Sounds scary! >In order to reduce freeze artifact using my system I have a few suggestions. >1) Cool the tissue and OCT to refriderator temp. I think this is a smart >idea for any > snap freezing technique. Why should we add the calories to go from room > temp to >0 degrees C, when it takes a lot less calories to go from 1 degree to O >degrees C. >2) My bars can be cooled down as low as about - 40 C and still maintain >the adhesive quality of the cold metal which is what allows us to be most >precise. Chucks can be put in >liquid nitrogen to get them super cooled. > >I would love to see someone try this and tell me how their result came >out. I think it would freeze pretty quickly. > >Any takers? Got to run for a frozen. > > >Stephen > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From mcclistd <@t> musc.edu Mon Mar 7 12:29:42 2005 From: mcclistd <@t> musc.edu (David McClister) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] mouse heart morphology Message-ID: Here is some more info about my situation with these mice hearts. Once the heart is extracted, it is put in saline 5 min, blotted dry, longitudinally cut and frozen in a mixture of ethanol and dry ice then stored in -80. The temperature of the crystat is -20. When the heart is cut, I cannot see the RV and LV chambers just one big heart. I'm doing a H&E and taking a 1x image to see the heart. Dentamold has been used in the past to keep the chambers separate but I could not get quality sections cutting through it. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks again, Dave McClister From katri <@t> cogeco.ca Mon Mar 7 12:41:41 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] PDGFR antibody Message-ID: <009e01c52345$4dbf1090$6a9a9618@Katri> Hi Histonetters, Has anyone used an antibody against PDGFR (platelet derived growth factor receptor) successfully on FFPE (formalin fixed paraffin embedded) human tissue? Could you let me know, which copany's antibody you used. Thank you! Katri Katri Tuomala Hamilton, Ontario, Canada From kbroomal <@t> NEMOURS.ORG Mon Mar 7 13:08:03 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] tried posting this once already... didn't work. Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A794E@wlmmsx01.nemours.org> Ok now.... "As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools." I'm sure someone else has already jumped on this one but, even though the numbers are diminishing, there are still colleges that offer degrees in Histotechnology. I just graduated from one last year and in fact am helping to teach this year's students. Here's a link: http://www.nsh.org/education/schools.html I have a Bachelor of Science and decided to return to school several years later to get an Associate of Science in Histotechnology. I think that my previous schooling only strengthens what I've learned in Histology. I don't know if I would retain half as much knowledge if I didn't already have a science background. I know that getting a 2 year degree after a 4 year degree is a bit backwards, but I'm pleased to tell you that I made a huge career leap forward by becoming a histotech. I've been a histotech for almost a year now & I can tell you that I'm still learning new things everyday and it is really rewarding to do stuff like finally get a ISH protocol right and be able to work out an immuno that just won't work right. I love what I do now and honestly, my 4 year degree helped me to get the histotech position that I have now. Just please don't put down the benefits of a 4 year degree. Knowledge is power. Kristen Broomall, HT (ASCP) -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: Friday, March 04, 2005 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tried posting this once already... didn't work. OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> bidmc.harvard.edu Mon Mar 7 13:27:28 2005 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] PKA antibody Message-ID: Hello, Could anyone recommend a good PKA antibody? I am looking for something that recognizes the C-alpha subunit and that is useful in mouse brain IHC or tissue culture. Any advice or suggestions would be appreciated. I would particularly like any hands on knowledge of the antibody in mouse tissue or western blot. Thanks, Caroline Bass Beth Israel Deaconess Medical Center From info <@t> instrumedics.com Mon Mar 7 13:48:01 2005 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] mouse heart morphology Message-ID: <00fc01c5234e$973d10b0$6401a8c0@INSTRUMEDICS22> David, If you freeze the heart on the Gentle Jane device and if you use the CryoJane Tape-Transfer process to prepare your frozen sections you can produce paraffin-quality frozen sections. Please visit our web site www.instrumedics.com to see the details. Click on the "gallery" to see many photomicrographs of CryoJane prepared frozen sections. You can request a CD which has a full demonstration of the tape-transfer process from snap-freezing the tissue on the Gentle Jane to fixation of the section We welcome any questions you may have. Bernice Instrumedics 800-237-2772 ----- Original Message ----- From: "David McClister" To: Sent: Monday, March 07, 2005 11:29 AM Subject: [Histonet] mouse heart morphology Hello, I am embedding longitudinal sectioned mice heart in OTC and need to have better morphology to be able to clearly see both left and right ventricles. This lab has tried dentamold but I found it very difficult to section. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks David McClister, HTL (ASCP) Medical University of South Carolina Dept of Cardiothoracic Research 117 Doughty St Charleston, SC 29425 From petepath <@t> yahoo.com Mon Mar 7 14:06:30 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] mouse heart morphology Message-ID: <20050307200630.14956.qmail@web30403.mail.mud.yahoo.com> David, Here is a idea based on no experience: Inject the fresh heart with OCT. Place a landmark with ink or suture to maintain orientation. Freeze it whole. Do either A or B A) Embed the entire heart in the desired orientation and simply trim down until you reach the desired plane of section. B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in the desired plane and embed the halves to achieve the desired plane of section. As far as getting high quality frozens section slides that is quite possible with good technique. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From tpmorken <@t> labvision.com Mon Mar 7 14:08:32 2005 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] RE: How people get into histology, and what education Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CA83@usca0082k08.labvision.apogent.com> Megan, Probably on the order of 99 percent of histotechs "fall" into the field. I travel around a lot and it is very, very rare to meet someone from the US who went through a formal program (and there are 4-year as well as 2-year programs). This is both a benefit and curse for histotechnology. A benefit because it means literally anyone with very basic biology and chemistry background can get into it, and it draws in a very diverse group of people. A curse because it means that few of those histotechs have the background to become well-versed in the field (as evidenced by the quantity of very basic questions on Histonet). In the past most histotechs did not become certifed by ASCP. I think that is changingnow , but most still only learn what they need for the job at hand. And most people in school never hear about the profession because of the lack of programs. Because of that the field does not draw well from the pool of people that are available for, and would be interested in this kind of work. Of course the current shortage is great for those in the field now - higher pay, pick your job, etc. You're right that for most hisotechnology work a AA degree is fine. In fact, it may be better because there are more openings for bench workers than for supervisory level people. It just depends on your goals. Even the vaunted Genentech biotech company has found that hiring AA-degree people is better for their business because they stay longer. They used to have a policy of only hiring BA/BS at a minimum, but found turnover was way too high for those people (average of two years). AA-degree people, for whatever reason, are more interested in staying in one job longer. It seems there is an annual Histonet discussion of the merits of on-the-job training (OJT) verses academic training. Of course, both are necessary and both contribute to success. We can find examples of both doing better or worse than others in the field. I've seen both groups do very well, and can tell horror stores of labs with the worst of each. The point is that the vast majority of histotechs fall into the position and learn on the job. But, from my own experience in working with may people, in general, those with more academic training are going to have the background to learn new things faster and maybe do better in more advanced technologies. For certain jobs that will be a key advantage. My advice to anyone in the field is to take your job description as a suggestion only and don't be tied down to it. Learn everything there is to do in the lab and take the opportunities as they come. It will be a much more interesting job and can take you to some interesting places! Tim Morken -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: Friday, March 04, 2005 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tried posting this once already... didn't work. OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelly.mcqueeney <@t> bms.com Mon Mar 7 14:08:36 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] PDGFR antibody In-Reply-To: <009e01c52345$4dbf1090$6a9a9618@Katri> References: <009e01c52345$4dbf1090$6a9a9618@Katri> Message-ID: <422CB4C4.2060604@bms.com> Hi Katria, I have used phospho-PDGFR antibody from Abcam (ab5511) on formalin-fixed paraffin sections of human kidney. You have to incubate O/N at 1:100 dilution and use TBS-tween as a wash buffer. Kelly Katri Tuomala wrote: > Hi Histonetters, > Has anyone used an antibody against PDGFR (platelet derived growth > factor receptor) successfully on FFPE (formalin fixed paraffin > embedded) human tissue? Could you let me know, which copany's antibody > you used. Thank you! > Katri > > Katri Tuomala > Hamilton, Ontario, Canada > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Mar 7 14:45:27 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:44 2005 Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology In-Reply-To: <20050307200630.14956.qmail@web30403.mail.mud.yahoo.com> References: <20050307200630.14956.qmail@web30403.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20050307133842.01b51640@gemini.msu.montana.edu> Filling the heart with OCT and then snap freezing is an excellent suggestion, and basically the same as filling mouse lungs with OCT to get stable, gently distended alveoli and excellent sectioning quality. When you bisect the heart after OCT fill and snap freeze, a room temperature teflon coated razor blade (EBS of Ted Pella or a teflon coated disposable microtome blade slices through frozen tissue/OCT smoothly, evenly plus the blades are very thin. We slice frozen tissue blocks frequently this way, without tissue damage. Gayle Callis At 01:06 PM 3/7/2005, you wrote: >David, > >Here is a idea based on no experience: > >Inject the fresh heart with OCT. Place a landmark with ink or suture to >maintain orientation. >Freeze it whole. Do either A or B > >A) Embed the entire heart in the desired orientation and simply trim down >until you reach the desired plane of section. > >B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in >the desired plane and embed the halves to achieve the desired plane of section. > >As far as getting high quality frozens section slides that is quite >possible with good technique. > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelly.mcqueeney <@t> bms.com Mon Mar 7 16:08:51 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:44 2005 Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology In-Reply-To: <6.0.0.22.1.20050307133842.01b51640@gemini.msu.montana.edu> References: <20050307200630.14956.qmail@web30403.mail.mud.yahoo.com> <6.0.0.22.1.20050307133842.01b51640@gemini.msu.montana.edu> Message-ID: <422CD0F3.5010207@bms.com> This may sound weird, but has anyone ever perfused a rodent with OCT (or a dilution of OCT) for better quality fresh brain tissue (we want to avoid fixation)? Thanks, Kelly Gayle Callis wrote: > Filling the heart with OCT and then snap freezing is an excellent > suggestion, and basically the same as filling mouse lungs with OCT to > get stable, gently distended alveoli and excellent sectioning quality. > > When you bisect the heart after OCT fill and snap freeze, a room > temperature teflon coated razor blade (EBS of Ted Pella or a teflon > coated disposable microtome blade slices through frozen tissue/OCT > smoothly, evenly plus the blades are very thin. We slice frozen > tissue blocks frequently this way, without tissue damage. > > Gayle Callis > > At 01:06 PM 3/7/2005, you wrote: > >> David, >> >> Here is a idea based on no experience: >> >> Inject the fresh heart with OCT. Place a landmark with ink or suture >> to maintain orientation. >> Freeze it whole. Do either A or B >> >> A) Embed the entire heart in the desired orientation and simply trim >> down until you reach the desired plane of section. >> >> B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut >> it in the desired plane and embed the halves to achieve the desired >> plane of section. >> >> As far as getting high quality frozens section slides that is quite >> possible with good technique. >> >> Stephen >> >> >> Stephen Peters M.D. >> Pathology Innovations, LLC >> 410 Old Mill Lane, >> Wyckoff, NJ 07481 >> 201 847 7600 >> www.pathologyinnovations.com >> >> Senior Attending Pathologist >> Hackensack University Medical Center >> 201 996 4836 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellinr <@t> amgen.com Mon Mar 7 16:17:32 2005 From: koellinr <@t> amgen.com (Koelling, Ray) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] RE: Excellent suggestions on filling heart for better (Re: [Histo net]) mouse heart morphology Message-ID: <16834C6DFFA6004C88DE4507FB8AE544018CC504@wa-mb4-sea.amgen.com> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Monday, March 07, 2005 12:45 PM To: Stephen Peters M.D.; Histonet@lists.utsouthwestern.edu Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology Filling the heart with OCT and then snap freezing is an excellent suggestion, and basically the same as filling mouse lungs with OCT to get stable, gently distended alveoli and excellent sectioning quality. When you bisect the heart after OCT fill and snap freeze, a room temperature teflon coated razor blade (EBS of Ted Pella or a teflon coated disposable microtome blade slices through frozen tissue/OCT smoothly, evenly plus the blades are very thin. We slice frozen tissue blocks frequently this way, without tissue damage. Gayle Callis At 01:06 PM 3/7/2005, you wrote: >David, > >Here is a idea based on no experience: > >Inject the fresh heart with OCT. Place a landmark with ink or suture to >maintain orientation. >Freeze it whole. Do either A or B > >A) Embed the entire heart in the desired orientation and simply trim down >until you reach the desired plane of section. > >B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in >the desired plane and embed the halves to achieve the desired plane of section. > >As far as getting high quality frozens section slides that is quite >possible with good technique. > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aetechserv <@t> hotmail.com Mon Mar 7 18:51:13 2005 From: aetechserv <@t> hotmail.com (awdaf asdfadf) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] workshops & society meeting attendees In-Reply-To: Message-ID: I agree ! Ventana is excellent about having knowledgeable speakers attend society meetings. Other Companies like Dako are also OK. Both have a high profile and professional presence. I've not heard of Vision Biosystems speaking at too many society meetings. They seem amateurish compared to the other 2. Since their house-cleaning last year, the prospects, from all accounts, are not great. The best sales people were let go to make room for a party group that spends more time socializing than attending to business. How many Companies can one person run into the ground ? Hope to hear Ventana at a future meeting. >From: "Sebree Linda A." >To: , >Subject: RE: [Histonet] Ventana workshops >Date: Fri, 4 Mar 2005 08:28:21 -0600 > >Angela, > >We have found Ventana very receptive to such requests. They are more than >happy to provide speakers/workshop leaders for state society meetings. So >I suggest you contact them before your next meeting to see if something >can be arranged. I know Ethel Macrea is an excellent, experienced speaker >for just these sort of situations. > >Linda A. Sebree >University of Wisconsin Hospital & Clinics >IHC/ISH Clinical & Research Laboratory >DM223-VA >600 Highland Ave. >Madison, WI 53792 >(608)265-6596 >FAX: (608)262-7174 > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela M. >Schmidt >Sent: Thursday, March 03, 2005 5:18 PM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Ventana workshops > > >Julie, > I think that it would be great if Ventana could put on a basic IHC and >ISH workshop. We have their technology but understanding the basics in this >growing part of our field would be greatly appreciated!! >Ventana---Southwest OHIO needs you!!! > > > >Angela M. Schmidt HT(ASCP) >Lead Histology Technician >TriHealth Laboratories >Good Samaritan-Bethesda Hospitals >Cincinnati, Ohio >513-872-1508 >a.schmidt@fuse.net > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar – get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ From yang5yc <@t> yahoo.com Mon Mar 7 19:01:14 2005 From: yang5yc <@t> yahoo.com (Kelly Yang) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] immunofluorescence staining in frozen tissue Message-ID: <20050308010114.63578.qmail@web52907.mail.yahoo.com> Dear histonetters, We are working on immunofluorescence staining with two markers, ki67 and DNMT1, in human bladder tissue. I had tried ki67 from Dako and DNMT1 from Imgenex, but none of them give us the good result. Does anyone have a protocol for staining frozen tissue with Ki67 or DNMT1? Also which antibody are you recommending? Thank you for your help in advance. Kelly Yang Graduate student Department of Epidemiology School of Public Heath University of California, Los Angeles 310-409-9179 ext. 57795 yangyc@ucla.edu __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From pigletbmw <@t> hotmail.com Mon Mar 7 19:24:08 2005 From: pigletbmw <@t> hotmail.com (alyssa piche) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Job Openings at UConn Message-ID: Hi Everyone, Just wanted to let everyone know that there are two part-time histotech positions at UConn Health Center. Check out their website at www.uchc.edu. Tina _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar – get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ From Amira.Fitieh <@t> neuro.gu.se Mon Mar 7 20:31:27 2005 From: Amira.Fitieh <@t> neuro.gu.se (Amira Fitieh) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Mast cells staining with TOL Message-ID: Hi all, We are looking for the Mast cells in rat brain. I have free floating tissue sections. The whole brain is fixed in formalin, sucrose and finally cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know what would be the most suitable protocol for staining the Mast cells with Toludine blue. I would also like to know if I could use positively charged glass slides instead of gelatin coated. Finally, I would like to know the most stable concentration for Toludine blue stock solution. Best regards, Amira ********************************************************************************************************************************** Amira Fitieh Research student Arvid Carlsson Institute for Neuroscience G?teborg University Medicinaregata 11 Box 432 405 30 G?teborg *********************************************************************************************************************************** From SURGPATH19 <@t> aol.com Mon Mar 7 21:51:36 2005 From: SURGPATH19 <@t> aol.com (SURGPATH19@aol.com) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] How do I Unsubscribe? Message-ID: <1d9.3806d79a.2f5e7b48@aol.com> From lpwenk <@t> sbcglobal.net Tue Mar 8 03:50:15 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] JACHO Compliance References: <7F1312711CA7474A89B3DF8BA0BA54D0F5F190@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <007801c523c4$3c48e6e0$1e2ad445@domainnotset.invalid> The quote from 2005 JCAHO regulations for laboratories is as follows: http://www.jcaho.org/accredited+organizations/laboratory+services/npsg/05_np sg_lab.htm "Use at least two patient identifiers (neither to be the patient's location) whenever collecting laboratory samples or administering medications or blood products, and use two identifiers to label sample collection containers in the presence of the patient. Processes are established to maintain samples' identity throughout the pre-analytical, analytical and post-analytical processes." Note that this is "whenever COLLECTING laboratory samples". In two different JCAHO laboratory FAQ: http://www.jcaho.org/accredited+organizations/laboratory+services/npsg/npsq_ faqs_2005.htm "Does the requirement to label all specimens with two identifiers in the presence of the patient apply to specimens collected by outside sources, such as physician offices? Yes. It is the intent of this goal to apply to all laboratory specimens. Laboratories may have limited ability to oversee this portion of the pre-analytical process, however, it is expected that all specimens arrive in the laboratory with a minimum of two identifiers on them. (New 1/18/05)" "Do the two sample identifiers have to be placed on every sample cup, container or aliquot used during the analytical process? No. When possible, laboratories are encouraged to label all aliquots with the two sample identifiers. However, it is impractical to expect this for all test systems due to space limitations on smaller sample cups and containers. As long as the samples' identity is maintained throughout the analytical process, this is acceptable. For example, identity is often maintained through use of an accession number or assigned position numbers on an analyzer. (New 1/18/05)" My interpretation is that this ruling applies to the COLLECTION of specimens in the OR, patients' rooms, doctors' offices. This ruling of needing two identifiers does not apply to the specimen once it is in the lab. However, there has to be a policy about accepting or rejecting specimens if they do not have two identifiers (from same FAQ 2005 page): "Does the Joint Commission require laboratories to reject specimens that arrive in the laboratory without two identifiers? No. While this may be an appropriate response for some situations, rejection and recollection may not always be an acceptable option for samples that are irretrievable, problematic or expensive to recollect (Pap smears), or when rejection will produce a significant treatment delay. The standards have previously required laboratories to establish written guidelines for specimen rejection. Such a policy may include a process for completing the specimen identification when recollection is not a reasonable option. It would be appropriate to include a cautionary statement on the laboratory report indicating the specimen was received in the laboratory without complete identification. (New 1/18/05)" Hope this helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Scholz, Stephen J." To: Sent: Friday, February 25, 2005 12:01 PM Subject: [Histonet] JACHO Compliance Hello all; We are about to have a JACHO inspection and a problem was brought to my attention. It involves using two identifiers on all laboratory specimens. The below paragraph is from JCAHO FAQ web site for laboratory compliance with the National Patient Safety Goals. This particular statement seems to answer the question about use of only one identifying number on a pathology or any lab specimen. Please let me know your interpretation and how your laboratories comply to it. Currently we have only the case number (hand written) on the blocks and only the case number (hand written) on the slides until after staining at which point there are printed labels put on them. Take note that this FAQ was updated in January, 2005. FAQ-We use a label for the initial patient sample that contains only one unique identifier, a number, to identify laboratory specimens. This unique identifier can be traced to multiple patient identifiers. Does this meet the intent of the goal? ANS-No. The intent is to use two separate identifiers on the specimen label. Confidence in an accurate identification improves as the number of identifiers increases, depending upon their uniqueness. A single identifier can be more easily misread, resulting in avoidable errors. Bar coding that includes two or more person-specific identifiers (not room number) will comply with this requirement. (New 1/18/05) What are suggestions to my problem. Stephen J. Scholz HT(ASCP) Histology Coordinator OSF St. Anthony Medical Center Rockford IL Phone: 815-395-5410 Fax: 815-395-5364 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From luckard <@t> verizon.net Tue Mar 8 03:46:49 2005 From: luckard <@t> verizon.net (luckard@verizon.net) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] (no subject) Message-ID: <0ID100D7K1U1HD62@vms048.mailsrvcs.net> From JOHN.PHILLIPS <@t> new-tr.wales.nhs.uk Tue Mar 8 04:00:52 2005 From: JOHN.PHILLIPS <@t> new-tr.wales.nhs.uk (JOHN PHILLIPS) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] IHC humidity chamber? Message-ID: <166A1E642B5B644DA694C08FD29D0ADC3E1384@ztroy.new-tr.wales.nhs.uk> Thermo Shandon supply this type of chamber, holds about 8 to 10 slides,if my memory serves me well - THISTIME!!! John, Wrexham, Wales, UK. -----Original Message----- From: J m [mailto:kosmicdog@hotmail.com] Sent: 07 March 2005 16:27 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC humidity chamber? salut, Does anyone know of a supplier in Canada for the IHC humidity chambers. I used to have a black plastic one with a clear lid but I don't know where it came from or where i can get another one. I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Regular tissue sections seem to "hold" the solution on the slide better than tissue arrays which are more apt to losing there solutions. I don't like using water repelent pens to circle the arrays but any suggestions would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu atynt. Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r rheolwr systemau wybod drwy ddefnyddio'r manylion isod. Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod, felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain Cymru. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau?r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru ar www.newalesnhstrust.org.uk This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager using the details below. The contents of this email represent the views of the individual(s) named above and do not necessarily represent the views of the North East Wales NHS Trust. Please be aware that, under the terms of the Freedom of Information Act 2000, the North East Wales NHS Trust may be required to make public the content of any emails or correspondence received. For futher information on Freedom of Information, please refer to the North East Wales NHS Trust website at www.newalesnhstrust.org.uk For further assistance, please contact system.administrator@new-tr.wales.nhs.uk. From lpwenk <@t> sbcglobal.net Tue Mar 8 04:21:20 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] HT pass rates Message-ID: <009901c523c8$930d9760$1e2ad445@domainnotset.invalid> Hi - My email server has been down, so I'm wading into this discussion late. Hope you all don't mind me putting in some statistics into some of the questions/comments. As for the pass rates of the HT exam: 1. July-Dec. 2004 - not yet published, so I don't know where the 75% failure rate figure is coming from for the "last exam". For Jan-June 2004, the HT pass rate was 49%. (that's those people who passed both the written and practical) 2. Typical pass rates: Usually in the range of 45-55%, from my looking back at the last several years of stats. So the first half of 2004 was in the same range. 3. Look back - in the years that followed the advent of DRG's (1984) and CLIA '88 (with the 1994 deadlines), many people who had been working in the field for many years, were scared that they might need to be certified. So they took the exam "at the last minute", without studying, and the pass rates did dip. If I were a betting person, I would be willing to gamble that, when we get the July-Dec 2004 stats, the pass rate is lower than usual, just based on my past experience with phone calls I received after " DRG and CLIA", and the ones I'm receiving now. People not passing the exam, wanting to know: - what books to buy (didn't look at the booklist they received, nor did they study from a book), - what topics to study (didn't look at the outline they received), - do they really HAVE to know all the chemicals in all the stains, or even, - do they have to know all the histology stains if they only do 6? They just figured, since they had been working in the field of "X" years, they knew it all. Sigh. Yes, some that didn't pass say they did study and tell me the books they studied from. But many didn't study, or didn't study enough, or didn't study the right material. So I try to help them over the phone, in my "free time", whatever that is. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lpwenk <@t> sbcglobal.net Tue Mar 8 04:32:47 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] PA Message-ID: <00a701c523ca$2c1ea920$1e2ad445@domainnotset.invalid> Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lpwenk <@t> sbcglobal.net Tue Mar 8 05:07:37 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] hardness of HT exam Message-ID: <00b501c523cf$0a53e580$1e2ad445@domainnotset.invalid> The question was raised - Has the HT (and HTL) exam has gotten harder over the years?. Yes and No, from my point of view. Yes - because there are always new topics. Things that were NOT on my certification exam (many years ago) but that I have to teach now: - AIDS, various hepatitis, PCP, Legionella - recycling - lot more IHC - automated equipment (coverslippers, H&E stainers, special stainers) - new stains - safety - regulations (CLIA, CAP, JCAHO) Every year, I add 1 or 2 new lectures, just to keep up with the changes in our field. But that's the natural progression of any medical field. No - because many of the questions are the same. If it was a good question about GMS/fungus 10 years ago, it is still a good question, and is still being used. Many of the questions in the exam pools have been around for years (decades even). My students talk to me after the exam, and they mention the same questions. No - because all the questions are benchmarked. That means - the ASCP histology exam committee knows the pass rate of the "established" questions. These questions have consistently had the same percent of people pass each time these questions are used. When a new question is used, it's pass rate is measured against "established" questions used on the same exam. If a question is out of it's "normal" range, that question is not counted on that exam, and the exam committee reviews the question, to see why it's pass rate changed. (old technology, typing error, whatever) No - because some of the questions on the exams are "test" questions - in other words, the committee is trying a couple out for the first time, to get statistics as to pass rate. These questions are NOT used in the applicants' pass rate. Some of these questions may be very tough on purpose, on new technology, or poorly written (not intentionally, but realized after re-evaluation based on the pass rate for that question), etc. But there have always been questions being tested for the first time. So this is nothing new. So why the perceptions that the exam is harder in the past year? My opinion - a lot of people are taking the exam to meet the 2005 associate degree deadline. In the years past (not including the 2003/2004 rush to take the exam because of the associate degree deadline), about 600 people per year were attempting the exam with about a 50% pass rate. So about 300 would not pass. In 2004, there were 480 that took the exam the first half of the year, and the numbers for the second have were reported via histonet to be around 1000. That means almost 1500 people took the HT exam last year, or more than double the usual number. And, the usual pass rate for the HT exam over the years is about 50%. So, based on past years, we can expect more numbers of people failing in 2004 (700+) than usually even attempt to take the exam in any other year. (Remember, this is my opinion at this time, and not based on any statistics that have been published.) Plus, Histonet is letting a larger community of people hear from people who have failed, or who work with someone who fails. So many histonetters are hearing the failure stories for the first time. As a program director, I've been hearing from people for years (and years). My opinion is that the failure rate has been constant for the past years. I'd love to hear from other program directors about this. I'll let everyone know the pass rate for July-Dec 2004, when they come out. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lsb <@t> jax.org Tue Mar 8 07:02:00 2005 From: lsb <@t> jax.org (Lesley S. Bechtold) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Veterinary Pathologist needed Message-ID: <6.2.1.2.2.20050308075837.02c6b2a0@aretha.jax.org> Hi Everyone, We are looking for a veterinary pathologist. We're located in Bar Harbor ME and work primarily with mice. The official posting and contact information is below. We're anxious to have our own pathologist on board. Thank you! Lesley Board Certified Veterinary Pathologist The Jackson Laboratory is seeking a board-certified veterinary pathologist with extensive experience in the pathology of laboratory mice to join its Laboratory Animal Health Services (LAHS) department. LAHS provides a variety of veterinary, diagnostic, and quality management services supporting the Research, Resource, and Mouse Service divisions of The Jackson Laboratory facility in Bar Harbor, Maine. The pathologist will provide anatomic pathology support for the internal sick mouse/health surveillance program, will mentor internal pathology trainees and otherwise participate in the educational mission of the Laboratory, and will interact with the Laboratory's scientific staff on a fee-for-service basis. Staff interactions will include phenotyping mutant mice, interpreting experimental results and consulting with staff members for the design and implementation of experiments. In addition, the pathologist will work with the Manager, Necropsy Service to develop and refine standard operating procedures for the Necropsy Service and to provide guidance in necropsy techniques and procedures for the pathology technicians. Provided that grant funding is available to cover this effort, up to 20% of the pathologist's time may be devoted to personal and/or collaborative research. The incumbent will also interact on a routine basis LAHS veterinarians specializing in microbiology, epidemiology, and laboratory animal medicine, and with The Jackson Laboratory's renowned mouse pathologists. This position requires excellent interpersonal and communication skills, a strong work ethic, and the ability to work in a diverse and energetic scientific community. Preference will be given to candidates who are eligible for licensure in the state of Maine. For consideration please send a curriculum vitae, the names and addresses of three professional references and a letter describing your qualifications for the position, and interest in this program to Dr. Peggy Danneman, Sr. Director, LAHS, The Jackson Laboratory, 600 Main St, Bar Harbor, ME 04609. For more than 70 years The Jackson Laboratory, an AAALAC-accredited, non-profit, independent research institution, has been dedicated to advancing human health through basic research in mammalian genetics. The Laboratory supplies resources and information to universities and research laboratories around the world and is a renowned education and training center. We are an equal opportunity employer situated in the small Maine coast town of Bar Harbor, adjacent to Acadia National Park. The surrounding area offers unlimited opportunities for outdoor activities, including hiking, biking, boating, kayaking and fishing. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From TrudgeonD <@t> rvh.on.ca Tue Mar 8 07:42:23 2005 From: TrudgeonD <@t> rvh.on.ca (Trudgeon, Debbie) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 14 Message-ID: <7969C47511F68542A97B2A721B8B2D7002296C3A@rvh-mail.rvh.on.ca> Hi, About 3 weeks ago, I subscribed to Histonet and I submitted a question. I have not heard back or seen anything about it. Does this mean the moderator turned it down? The question was - What specimen sites are other labs running a Giemsa stain on? We currently run them daily on esophagus, stomach, and duodenum. Thanks, Debbie Debbie Trudgeon Charge Technologist Histology Royal Victoria Hospital 201 Georgian Drive Barrie, ON L4M 6M2 (705) 728-9090 x6446 trudgeond@rvh.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 08, 2005 8:39 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: mouse heart morphology (Gayle Callis) 2. mouse heart morphology (David McClister) 3. PDGFR antibody (Katri Tuomala) 4. RE: tried posting this once already... didn't work. (Kristen Broomall) 5. PKA antibody (Caroline Bass) 6. mouse heart morphology (Instrumedics) 7. mouse heart morphology (Stephen Peters M.D.) 8. RE: How people get into histology, and what education (Morken, Tim - Labvision) 9. Re: PDGFR antibody (Kelly D Mcqueeney) 10. Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology (Gayle Callis) 11. Re: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology (Kelly D Mcqueeney) 12. RE: Excellent suggestions on filling heart for better (Re: [Histo net]) mouse heart morphology (Koelling, Ray) 13. RE: workshops & society meeting attendees (awdaf asdfadf) 14. immunofluorescence staining in frozen tissue (Kelly Yang) 15. Job Openings at UConn (alyssa piche) 16. Mast cells staining with TOL (Amira Fitieh) 17. How do I Unsubscribe? (SURGPATH19@aol.com) 18. Re: JACHO Compliance (lpwenk@sbcglobal.net) 19. (no subject) (luckard@verizon.net) 20. RE: IHC humidity chamber? (JOHN PHILLIPS) 21. HT pass rates (lpwenk@sbcglobal.net) 22. PA (lpwenk@sbcglobal.net) 23. hardness of HT exam (lpwenk@sbcglobal.net) 24. Veterinary Pathologist needed (Lesley S. Bechtold) ---------------------------------------------------------------------- Message: 1 Date: Mon, 07 Mar 2005 11:25:04 -0700 From: Gayle Callis Subject: Re: [Histonet] mouse heart morphology To: "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050307105927.01b45ab8@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Stephen, Sorry buy -40C is NOT cold enough for freezing our murine tissues. I believe that others have experienced this problem also, the one of getting freezing artifact/damaged morphology with inside cryostat temperature freezing. Nothing scary about liquid nitrogen usage, although Isopentane is more of a worry for storage. I was thinking more of precooling the metal device containing wells i.e surround the device with liquid nitrogen, then you have the wells at the extremely cold temperatures. Liquid nitrogen in the wells would not be ideal, to be sure. We do this with a metal block sitting in liquid nitrogen so the metal block is at liquid nitrogen temperatures, then place an OCT embedded murine tissue (inside a Tissue Tek plastic mold) on top of the very cold metal block. This permits murine tissue to freeze at a much colder temperature and without artifact. I have seen your device and like its design, but if mouse tissue cannot be snap frozen at colder temperature than -40C, then we can't use it due to terrible morphology. Cryostat freezing temperatures for murine cryomicrotomy simply do not do the job well enough. It is the water ice crystal formation inside the tissue that presents the big problem and the colder the freezing temperature, the smaller the ice crystal formation - hence snap freezing in the true sense of the words. Precooling OCT and a tissue is not feasible when you have 10 mice lined up and collecting 15 tissues out of each mouse as fast as you can dissect and snap freeze plus the actual snap freezing takes only seconds or so - extremely fast. I would love to try your device in the way we use our metal block surround by liquid nitrogen as your device has a very efficient design. There is no doubt it works very well for clinical laboratories for rapid diagnostic work, but for our fussy murine tissues, colder temperatures must prevail. There is an excellent discussion by Charles Scouten on snap freezing biological samples can be found at www.myneurolab.com with details on ice crystal formation, etc. - what we experience with murine tissue. Gayle Callis At 10:52 AM 3/7/2005, you wrote: >HI Gail, > >I have not experimented with liquid nitrogen in my wells. Sounds scary! >In order to reduce freeze artifact using my system I have a few suggestions. >1) Cool the tissue and OCT to refriderator temp. I think this is a smart >idea for any > snap freezing technique. Why should we add the calories to go from room > temp to >0 degrees C, when it takes a lot less calories to go from 1 degree to O >degrees C. >2) My bars can be cooled down as low as about - 40 C and still maintain >the adhesive quality of the cold metal which is what allows us to be most >precise. Chucks can be put in >liquid nitrogen to get them super cooled. > >I would love to see someone try this and tell me how their result came >out. I think it would freeze pretty quickly. > >Any takers? Got to run for a frozen. > > >Stephen > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 2 Date: Mon, 07 Mar 2005 13:29:42 -0500 From: "David McClister" Subject: [Histonet] mouse heart morphology To: Message-ID: Content-Type: text/plain; charset=US-ASCII Here is some more info about my situation with these mice hearts. Once the heart is extracted, it is put in saline 5 min, blotted dry, longitudinally cut and frozen in a mixture of ethanol and dry ice then stored in -80. The temperature of the crystat is -20. When the heart is cut, I cannot see the RV and LV chambers just one big heart. I'm doing a H&E and taking a 1x image to see the heart. Dentamold has been used in the past to keep the chambers separate but I could not get quality sections cutting through it. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks again, Dave McClister ------------------------------ Message: 3 Date: Mon, 7 Mar 2005 13:41:41 -0500 From: "Katri Tuomala" Subject: [Histonet] PDGFR antibody To: Message-ID: <009e01c52345$4dbf1090$6a9a9618@Katri> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Histonetters, Has anyone used an antibody against PDGFR (platelet derived growth factor receptor) successfully on FFPE (formalin fixed paraffin embedded) human tissue? Could you let me know, which copany's antibody you used. Thank you! Katri Katri Tuomala Hamilton, Ontario, Canada ------------------------------ Message: 4 Date: Mon, 7 Mar 2005 14:08:03 -0500 From: Kristen Broomall Subject: RE: [Histonet] tried posting this once already... didn't work. To: "'TheBestTime23@aol.com'" , histonet@lists.utsouthwestern.edu Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A794E@wlmmsx01.nemours.org> Content-Type: text/plain; charset="iso-8859-1" Ok now.... "As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools." I'm sure someone else has already jumped on this one but, even though the numbers are diminishing, there are still colleges that offer degrees in Histotechnology. I just graduated from one last year and in fact am helping to teach this year's students. Here's a link: http://www.nsh.org/education/schools.html I have a Bachelor of Science and decided to return to school several years later to get an Associate of Science in Histotechnology. I think that my previous schooling only strengthens what I've learned in Histology. I don't know if I would retain half as much knowledge if I didn't already have a science background. I know that getting a 2 year degree after a 4 year degree is a bit backwards, but I'm pleased to tell you that I made a huge career leap forward by becoming a histotech. I've been a histotech for almost a year now & I can tell you that I'm still learning new things everyday and it is really rewarding to do stuff like finally get a ISH protocol right and be able to work out an immuno that just won't work right. I love what I do now and honestly, my 4 year degree helped me to get the histotech position that I have now. Just please don't put down the benefits of a 4 year degree. Knowledge is power. Kristen Broomall, HT (ASCP) -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: Friday, March 04, 2005 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tried posting this once already... didn't work. OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 7 Mar 2005 14:27:28 -0500 From: Caroline Bass Subject: [Histonet] PKA antibody To: " " Message-ID: Content-Type: text/plain; charset=US-ASCII; format=flowed Hello, Could anyone recommend a good PKA antibody? I am looking for something that recognizes the C-alpha subunit and that is useful in mouse brain IHC or tissue culture. Any advice or suggestions would be appreciated. I would particularly like any hands on knowledge of the antibody in mouse tissue or western blot. Thanks, Caroline Bass Beth Israel Deaconess Medical Center ------------------------------ Message: 6 Date: Mon, 7 Mar 2005 14:48:01 -0500 From: "Instrumedics" Subject: [Histonet] mouse heart morphology To: Message-ID: <00fc01c5234e$973d10b0$6401a8c0@INSTRUMEDICS22> Content-Type: text/plain; charset="iso-8859-1" David, If you freeze the heart on the Gentle Jane device and if you use the CryoJane Tape-Transfer process to prepare your frozen sections you can produce paraffin-quality frozen sections. Please visit our web site www.instrumedics.com to see the details. Click on the "gallery" to see many photomicrographs of CryoJane prepared frozen sections. You can request a CD which has a full demonstration of the tape-transfer process from snap-freezing the tissue on the Gentle Jane to fixation of the section We welcome any questions you may have. Bernice Instrumedics 800-237-2772 ----- Original Message ----- From: "David McClister" To: Sent: Monday, March 07, 2005 11:29 AM Subject: [Histonet] mouse heart morphology Hello, I am embedding longitudinal sectioned mice heart in OTC and need to have better morphology to be able to clearly see both left and right ventricles. This lab has tried dentamold but I found it very difficult to section. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks David McClister, HTL (ASCP) Medical University of South Carolina Dept of Cardiothoracic Research 117 Doughty St Charleston, SC 29425 ------------------------------ Message: 7 Date: Mon, 7 Mar 2005 12:06:30 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] mouse heart morphology To: histonet@lists.utsouthwestern.edu Message-ID: <20050307200630.14956.qmail@web30403.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii David, Here is a idea based on no experience: Inject the fresh heart with OCT. Place a landmark with ink or suture to maintain orientation. Freeze it whole. Do either A or B A) Embed the entire heart in the desired orientation and simply trim down until you reach the desired plane of section. B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in the desired plane and embed the halves to achieve the desired plane of section. As far as getting high quality frozens section slides that is quite possible with good technique. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 ------------------------------ Message: 8 Date: Mon, 7 Mar 2005 15:08:32 -0500 From: "Morken, Tim - Labvision" Subject: [Histonet] RE: How people get into histology, and what education To: "histonet (E-mail)" Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CA83@usca0082k08.labvision.apogent.com> Content-Type: text/plain Megan, Probably on the order of 99 percent of histotechs "fall" into the field. I travel around a lot and it is very, very rare to meet someone from the US who went through a formal program (and there are 4-year as well as 2-year programs). This is both a benefit and curse for histotechnology. A benefit because it means literally anyone with very basic biology and chemistry background can get into it, and it draws in a very diverse group of people. A curse because it means that few of those histotechs have the background to become well-versed in the field (as evidenced by the quantity of very basic questions on Histonet). In the past most histotechs did not become certifed by ASCP. I think that is changingnow , but most still only learn what they need for the job at hand. And most people in school never hear about the profession because of the lack of programs. Because of that the field does not draw well from the pool of people that are available for, and would be interested in this kind of work. Of course the current shortage is great for those in the field now - higher pay, pick your job, etc. You're right that for most hisotechnology work a AA degree is fine. In fact, it may be better because there are more openings for bench workers than for supervisory level people. It just depends on your goals. Even the vaunted Genentech biotech company has found that hiring AA-degree people is better for their business because they stay longer. They used to have a policy of only hiring BA/BS at a minimum, but found turnover was way too high for those people (average of two years). AA-degree people, for whatever reason, are more interested in staying in one job longer. It seems there is an annual Histonet discussion of the merits of on-the-job training (OJT) verses academic training. Of course, both are necessary and both contribute to success. We can find examples of both doing better or worse than others in the field. I've seen both groups do very well, and can tell horror stores of labs with the worst of each. The point is that the vast majority of histotechs fall into the position and learn on the job. But, from my own experience in working with may people, in general, those with more academic training are going to have the background to learn new things faster and maybe do better in more advanced technologies. For certain jobs that will be a key advantage. My advice to anyone in the field is to take your job description as a suggestion only and don't be tied down to it. Learn everything there is to do in the lab and take the opportunities as they come. It will be a much more interesting job and can take you to some interesting places! Tim Morken -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: Friday, March 04, 2005 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tried posting this once already... didn't work. OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 07 Mar 2005 15:08:36 -0500 From: Kelly D Mcqueeney Subject: Re: [Histonet] PDGFR antibody To: Katri Tuomala Cc: histonet@lists.utsouthwestern.edu Message-ID: <422CB4C4.2060604@bms.com> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Hi Katria, I have used phospho-PDGFR antibody from Abcam (ab5511) on formalin-fixed paraffin sections of human kidney. You have to incubate O/N at 1:100 dilution and use TBS-tween as a wash buffer. Kelly Katri Tuomala wrote: > Hi Histonetters, > Has anyone used an antibody against PDGFR (platelet derived growth > factor receptor) successfully on FFPE (formalin fixed paraffin > embedded) human tissue? Could you let me know, which copany's antibody > you used. Thank you! > Katri > > Katri Tuomala > Hamilton, Ontario, Canada > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 07 Mar 2005 13:45:27 -0700 From: Gayle Callis Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology To: "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050307133842.01b51640@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Filling the heart with OCT and then snap freezing is an excellent suggestion, and basically the same as filling mouse lungs with OCT to get stable, gently distended alveoli and excellent sectioning quality. When you bisect the heart after OCT fill and snap freeze, a room temperature teflon coated razor blade (EBS of Ted Pella or a teflon coated disposable microtome blade slices through frozen tissue/OCT smoothly, evenly plus the blades are very thin. We slice frozen tissue blocks frequently this way, without tissue damage. Gayle Callis At 01:06 PM 3/7/2005, you wrote: >David, > >Here is a idea based on no experience: > >Inject the fresh heart with OCT. Place a landmark with ink or suture to >maintain orientation. >Freeze it whole. Do either A or B > >A) Embed the entire heart in the desired orientation and simply trim down >until you reach the desired plane of section. > >B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in >the desired plane and embed the halves to achieve the desired plane of section. > >As far as getting high quality frozens section slides that is quite >possible with good technique. > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 07 Mar 2005 17:08:51 -0500 From: Kelly D Mcqueeney Subject: Re: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology To: Gayle Callis Cc: Histonet@lists.utsouthwestern.edu, "Stephen Peters M.D." Message-ID: <422CD0F3.5010207@bms.com> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 This may sound weird, but has anyone ever perfused a rodent with OCT (or a dilution of OCT) for better quality fresh brain tissue (we want to avoid fixation)? Thanks, Kelly Gayle Callis wrote: > Filling the heart with OCT and then snap freezing is an excellent > suggestion, and basically the same as filling mouse lungs with OCT to > get stable, gently distended alveoli and excellent sectioning quality. > > When you bisect the heart after OCT fill and snap freeze, a room > temperature teflon coated razor blade (EBS of Ted Pella or a teflon > coated disposable microtome blade slices through frozen tissue/OCT > smoothly, evenly plus the blades are very thin. We slice frozen > tissue blocks frequently this way, without tissue damage. > > Gayle Callis > > At 01:06 PM 3/7/2005, you wrote: > >> David, >> >> Here is a idea based on no experience: >> >> Inject the fresh heart with OCT. Place a landmark with ink or suture >> to maintain orientation. >> Freeze it whole. Do either A or B >> >> A) Embed the entire heart in the desired orientation and simply trim >> down until you reach the desired plane of section. >> >> B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut >> it in the desired plane and embed the halves to achieve the desired >> plane of section. >> >> As far as getting high quality frozens section slides that is quite >> possible with good technique. >> >> Stephen >> >> >> Stephen Peters M.D. >> Pathology Innovations, LLC >> 410 Old Mill Lane, >> Wyckoff, NJ 07481 >> 201 847 7600 >> www.pathologyinnovations.com >> >> Senior Attending Pathologist >> Hackensack University Medical Center >> 201 996 4836 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 7 Mar 2005 14:17:32 -0800 From: "Koelling, Ray" Subject: [Histonet] RE: Excellent suggestions on filling heart for better (Re: [Histo net]) mouse heart morphology To: "'Gayle Callis'" , "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <16834C6DFFA6004C88DE4507FB8AE544018CC504@wa-mb4-sea.amgen.com> Content-Type: text/plain; charset="iso-8859-1" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Monday, March 07, 2005 12:45 PM To: Stephen Peters M.D.; Histonet@lists.utsouthwestern.edu Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology Filling the heart with OCT and then snap freezing is an excellent suggestion, and basically the same as filling mouse lungs with OCT to get stable, gently distended alveoli and excellent sectioning quality. When you bisect the heart after OCT fill and snap freeze, a room temperature teflon coated razor blade (EBS of Ted Pella or a teflon coated disposable microtome blade slices through frozen tissue/OCT smoothly, evenly plus the blades are very thin. We slice frozen tissue blocks frequently this way, without tissue damage. Gayle Callis At 01:06 PM 3/7/2005, you wrote: >David, > >Here is a idea based on no experience: > >Inject the fresh heart with OCT. Place a landmark with ink or suture to >maintain orientation. >Freeze it whole. Do either A or B > >A) Embed the entire heart in the desired orientation and simply trim down >until you reach the desired plane of section. > >B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in >the desired plane and embed the halves to achieve the desired plane of section. > >As far as getting high quality frozens section slides that is quite >possible with good technique. > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 08 Mar 2005 00:51:13 +0000 From: "awdaf asdfadf" Subject: RE: [Histonet] workshops & society meeting attendees To: la.sebree@hosp.wisc.edu, a.schmidt@fuse.net, Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed I agree ! Ventana is excellent about having knowledgeable speakers attend society meetings. Other Companies like Dako are also OK. Both have a high profile and professional presence. I've not heard of Vision Biosystems speaking at too many society meetings. They seem amateurish compared to the other 2. Since their house-cleaning last year, the prospects, from all accounts, are not great. The best sales people were let go to make room for a party group that spends more time socializing than attending to business. How many Companies can one person run into the ground ? Hope to hear Ventana at a future meeting. >From: "Sebree Linda A." >To: , >Subject: RE: [Histonet] Ventana workshops >Date: Fri, 4 Mar 2005 08:28:21 -0600 > >Angela, > >We have found Ventana very receptive to such requests. They are more than >happy to provide speakers/workshop leaders for state society meetings. So >I suggest you contact them before your next meeting to see if something >can be arranged. I know Ethel Macrea is an excellent, experienced speaker >for just these sort of situations. > >Linda A. Sebree >University of Wisconsin Hospital & Clinics >IHC/ISH Clinical & Research Laboratory >DM223-VA >600 Highland Ave. >Madison, WI 53792 >(608)265-6596 >FAX: (608)262-7174 > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela M. >Schmidt >Sent: Thursday, March 03, 2005 5:18 PM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Ventana workshops > > >Julie, > I think that it would be great if Ventana could put on a basic IHC and >ISH workshop. We have their technology but understanding the basics in this >growing part of our field would be greatly appreciated!! >Ventana---Southwest OHIO needs you!!! > > > >Angela M. Schmidt HT(ASCP) >Lead Histology Technician >TriHealth Laboratories >Good Samaritan-Bethesda Hospitals >Cincinnati, Ohio >513-872-1508 >a.schmidt@fuse.net > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar - get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ ------------------------------ Message: 14 Date: Mon, 7 Mar 2005 17:01:14 -0800 (PST) From: Kelly Yang Subject: [Histonet] immunofluorescence staining in frozen tissue To: histonet@lists.utsouthwestern.edu Message-ID: <20050308010114.63578.qmail@web52907.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear histonetters, We are working on immunofluorescence staining with two markers, ki67 and DNMT1, in human bladder tissue. I had tried ki67 from Dako and DNMT1 from Imgenex, but none of them give us the good result. Does anyone have a protocol for staining frozen tissue with Ki67 or DNMT1? Also which antibody are you recommending? Thank you for your help in advance. Kelly Yang Graduate student Department of Epidemiology School of Public Heath University of California, Los Angeles 310-409-9179 ext. 57795 yangyc@ucla.edu __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 15 Date: Mon, 07 Mar 2005 20:24:08 -0500 From: "alyssa piche" Subject: [Histonet] Job Openings at UConn To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Hi Everyone, Just wanted to let everyone know that there are two part-time histotech positions at UConn Health Center. Check out their website at www.uchc.edu. Tina _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar - get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ ------------------------------ Message: 16 Date: Tue, 8 Mar 2005 03:31:27 +0100 From: "Amira Fitieh" Subject: [Histonet] Mast cells staining with TOL To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all, We are looking for the Mast cells in rat brain. I have free floating tissue sections. The whole brain is fixed in formalin, sucrose and finally cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know what would be the most suitable protocol for staining the Mast cells with Toludine blue. I would also like to know if I could use positively charged glass slides instead of gelatin coated. Finally, I would like to know the most stable concentration for Toludine blue stock solution. Best regards, Amira ********************************************************************************************************************************** Amira Fitieh Research student Arvid Carlsson Institute for Neuroscience G?teborg University Medicinaregata 11 Box 432 405 30 G?teborg *********************************************************************************************************************************** ------------------------------ Message: 17 Date: Mon, 7 Mar 2005 22:51:36 EST From: SURGPATH19@aol.com Subject: [Histonet] How do I Unsubscribe? To: histonet@lists.utsouthwestern.edu Message-ID: <1d9.3806d79a.2f5e7b48@aol.com> Content-Type: text/plain; charset="US-ASCII" ------------------------------ Message: 18 Date: Tue, 8 Mar 2005 04:50:15 -0500 From: Subject: Re: [Histonet] JACHO Compliance To: "Scholz, Stephen J." , Message-ID: <007801c523c4$3c48e6e0$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" The quote from 2005 JCAHO regulations for laboratories is as follows: http://www.jcaho.org/accredited+organizations/laboratory+services/npsg/05_np sg_lab.htm "Use at least two patient identifiers (neither to be the patient's location) whenever collecting laboratory samples or administering medications or blood products, and use two identifiers to label sample collection containers in the presence of the patient. Processes are established to maintain samples' identity throughout the pre-analytical, analytical and post-analytical processes." Note that this is "whenever COLLECTING laboratory samples". In two different JCAHO laboratory FAQ: http://www.jcaho.org/accredited+organizations/laboratory+services/npsg/npsq_ faqs_2005.htm "Does the requirement to label all specimens with two identifiers in the presence of the patient apply to specimens collected by outside sources, such as physician offices? Yes. It is the intent of this goal to apply to all laboratory specimens. Laboratories may have limited ability to oversee this portion of the pre-analytical process, however, it is expected that all specimens arrive in the laboratory with a minimum of two identifiers on them. (New 1/18/05)" "Do the two sample identifiers have to be placed on every sample cup, container or aliquot used during the analytical process? No. When possible, laboratories are encouraged to label all aliquots with the two sample identifiers. However, it is impractical to expect this for all test systems due to space limitations on smaller sample cups and containers. As long as the samples' identity is maintained throughout the analytical process, this is acceptable. For example, identity is often maintained through use of an accession number or assigned position numbers on an analyzer. (New 1/18/05)" My interpretation is that this ruling applies to the COLLECTION of specimens in the OR, patients' rooms, doctors' offices. This ruling of needing two identifiers does not apply to the specimen once it is in the lab. However, there has to be a policy about accepting or rejecting specimens if they do not have two identifiers (from same FAQ 2005 page): "Does the Joint Commission require laboratories to reject specimens that arrive in the laboratory without two identifiers? No. While this may be an appropriate response for some situations, rejection and recollection may not always be an acceptable option for samples that are irretrievable, problematic or expensive to recollect (Pap smears), or when rejection will produce a significant treatment delay. The standards have previously required laboratories to establish written guidelines for specimen rejection. Such a policy may include a process for completing the specimen identification when recollection is not a reasonable option. It would be appropriate to include a cautionary statement on the laboratory report indicating the specimen was received in the laboratory without complete identification. (New 1/18/05)" Hope this helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Scholz, Stephen J." To: Sent: Friday, February 25, 2005 12:01 PM Subject: [Histonet] JACHO Compliance Hello all; We are about to have a JACHO inspection and a problem was brought to my attention. It involves using two identifiers on all laboratory specimens. The below paragraph is from JCAHO FAQ web site for laboratory compliance with the National Patient Safety Goals. This particular statement seems to answer the question about use of only one identifying number on a pathology or any lab specimen. Please let me know your interpretation and how your laboratories comply to it. Currently we have only the case number (hand written) on the blocks and only the case number (hand written) on the slides until after staining at which point there are printed labels put on them. Take note that this FAQ was updated in January, 2005. FAQ-We use a label for the initial patient sample that contains only one unique identifier, a number, to identify laboratory specimens. This unique identifier can be traced to multiple patient identifiers. Does this meet the intent of the goal? ANS-No. The intent is to use two separate identifiers on the specimen label. Confidence in an accurate identification improves as the number of identifiers increases, depending upon their uniqueness. A single identifier can be more easily misread, resulting in avoidable errors. Bar coding that includes two or more person-specific identifiers (not room number) will comply with this requirement. (New 1/18/05) What are suggestions to my problem. Stephen J. Scholz HT(ASCP) Histology Coordinator OSF St. Anthony Medical Center Rockford IL Phone: 815-395-5410 Fax: 815-395-5364 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 08 Mar 2005 09:46:49 +0000 From: Subject: [Histonet] (no subject) To: Message-ID: <0ID100D7K1U1HD62@vms048.mailsrvcs.net> Content-Type: text/plain; charset=ISO-8859-1 ------------------------------ Message: 20 Date: Tue, 8 Mar 2005 10:00:52 -0000 From: "JOHN PHILLIPS" Subject: RE: [Histonet] IHC humidity chamber? To: "J m" , Message-ID: <166A1E642B5B644DA694C08FD29D0ADC3E1384@ztroy.new-tr.wales.nhs.uk> Content-Type: text/plain; charset="iso-8859-1" Thermo Shandon supply this type of chamber, holds about 8 to 10 slides,if my memory serves me well - THISTIME!!! John, Wrexham, Wales, UK. -----Original Message----- From: J m [mailto:kosmicdog@hotmail.com] Sent: 07 March 2005 16:27 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC humidity chamber? salut, Does anyone know of a supplier in Canada for the IHC humidity chambers. I used to have a black plastic one with a clear lid but I don't know where it came from or where i can get another one. I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Regular tissue sections seem to "hold" the solution on the slide better than tissue arrays which are more apt to losing there solutions. I don't like using water repelent pens to circle the arrays but any suggestions would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu atynt. Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r rheolwr systemau wybod drwy ddefnyddio'r manylion isod. Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod, felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain Cymru. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau?r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru ar www.newalesnhstrust.org.uk This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager using the details below. The contents of this email represent the views of the individual(s) named above and do not necessarily represent the views of the North East Wales NHS Trust. Please be aware that, under the terms of the Freedom of Information Act 2000, the North East Wales NHS Trust may be required to make public the content of any emails or correspondence received. For futher information on Freedom of Information, please refer to the North East Wales NHS Trust website at www.newalesnhstrust.org.uk For further assistance, please contact system.administrator@new-tr.wales.nhs.uk. ------------------------------ Message: 21 Date: Tue, 8 Mar 2005 05:21:20 -0500 From: Subject: [Histonet] HT pass rates To: "Histonet" Message-ID: <009901c523c8$930d9760$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Hi - My email server has been down, so I'm wading into this discussion late. Hope you all don't mind me putting in some statistics into some of the questions/comments. As for the pass rates of the HT exam: 1. July-Dec. 2004 - not yet published, so I don't know where the 75% failure rate figure is coming from for the "last exam". For Jan-June 2004, the HT pass rate was 49%. (that's those people who passed both the written and practical) 2. Typical pass rates: Usually in the range of 45-55%, from my looking back at the last several years of stats. So the first half of 2004 was in the same range. 3. Look back - in the years that followed the advent of DRG's (1984) and CLIA '88 (with the 1994 deadlines), many people who had been working in the field for many years, were scared that they might need to be certified. So they took the exam "at the last minute", without studying, and the pass rates did dip. If I were a betting person, I would be willing to gamble that, when we get the July-Dec 2004 stats, the pass rate is lower than usual, just based on my past experience with phone calls I received after " DRG and CLIA", and the ones I'm receiving now. People not passing the exam, wanting to know: - what books to buy (didn't look at the booklist they received, nor did they study from a book), - what topics to study (didn't look at the outline they received), - do they really HAVE to know all the chemicals in all the stains, or even, - do they have to know all the histology stains if they only do 6? They just figured, since they had been working in the field of "X" years, they knew it all. Sigh. Yes, some that didn't pass say they did study and tell me the books they studied from. But many didn't study, or didn't study enough, or didn't study the right material. So I try to help them over the phone, in my "free time", whatever that is. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 22 Date: Tue, 8 Mar 2005 05:32:47 -0500 From: Subject: [Histonet] PA To: "Histonet" Message-ID: <00a701c523ca$2c1ea920$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 23 Date: Tue, 8 Mar 2005 06:07:37 -0500 From: Subject: [Histonet] hardness of HT exam To: "Histonet" Message-ID: <00b501c523cf$0a53e580$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" The question was raised - Has the HT (and HTL) exam has gotten harder over the years?. Yes and No, from my point of view. Yes - because there are always new topics. Things that were NOT on my certification exam (many years ago) but that I have to teach now: - AIDS, various hepatitis, PCP, Legionella - recycling - lot more IHC - automated equipment (coverslippers, H&E stainers, special stainers) - new stains - safety - regulations (CLIA, CAP, JCAHO) Every year, I add 1 or 2 new lectures, just to keep up with the changes in our field. But that's the natural progression of any medical field. No - because many of the questions are the same. If it was a good question about GMS/fungus 10 years ago, it is still a good question, and is still being used. Many of the questions in the exam pools have been around for years (decades even). My students talk to me after the exam, and they mention the same questions. No - because all the questions are benchmarked. That means - the ASCP histology exam committee knows the pass rate of the "established" questions. These questions have consistently had the same percent of people pass each time these questions are used. When a new question is used, it's pass rate is measured against "established" questions used on the same exam. If a question is out of it's "normal" range, that question is not counted on that exam, and the exam committee reviews the question, to see why it's pass rate changed. (old technology, typing error, whatever) No - because some of the questions on the exams are "test" questions - in other words, the committee is trying a couple out for the first time, to get statistics as to pass rate. These questions are NOT used in the applicants' pass rate. Some of these questions may be very tough on purpose, on new technology, or poorly written (not intentionally, but realized after re-evaluation based on the pass rate for that question), etc. But there have always been questions being tested for the first time. So this is nothing new. So why the perceptions that the exam is harder in the past year? My opinion - a lot of people are taking the exam to meet the 2005 associate degree deadline. In the years past (not including the 2003/2004 rush to take the exam because of the associate degree deadline), about 600 people per year were attempting the exam with about a 50% pass rate. So about 300 would not pass. In 2004, there were 480 that took the exam the first half of the year, and the numbers for the second have were reported via histonet to be around 1000. That means almost 1500 people took the HT exam last year, or more than double the usual number. And, the usual pass rate for the HT exam over the years is about 50%. So, based on past years, we can expect more numbers of people failing in 2004 (700+) than usually even attempt to take the exam in any other year. (Remember, this is my opinion at this time, and not based on any statistics that have been published.) Plus, Histonet is letting a larger community of people hear from people who have failed, or who work with someone who fails. So many histonetters are hearing the failure stories for the first time. As a program director, I've been hearing from people for years (and years). My opinion is that the failure rate has been constant for the past years. I'd love to hear from other program directors about this. I'll let everyone know the pass rate for July-Dec 2004, when they come out. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 24 Date: Tue, 08 Mar 2005 08:02:00 -0500 From: "Lesley S. Bechtold" Subject: [Histonet] Veterinary Pathologist needed To: histonet@Pathology.swmed.edu Message-ID: <6.2.1.2.2.20050308075837.02c6b2a0@aretha.jax.org> Content-Type: text/plain; charset="us-ascii"; format=flowed Hi Everyone, We are looking for a veterinary pathologist. We're located in Bar Harbor ME and work primarily with mice. The official posting and contact information is below. We're anxious to have our own pathologist on board. Thank you! Lesley Board Certified Veterinary Pathologist The Jackson Laboratory is seeking a board-certified veterinary pathologist with extensive experience in the pathology of laboratory mice to join its Laboratory Animal Health Services (LAHS) department. LAHS provides a variety of veterinary, diagnostic, and quality management services supporting the Research, Resource, and Mouse Service divisions of The Jackson Laboratory facility in Bar Harbor, Maine. The pathologist will provide anatomic pathology support for the internal sick mouse/health surveillance program, will mentor internal pathology trainees and otherwise participate in the educational mission of the Laboratory, and will interact with the Laboratory's scientific staff on a fee-for-service basis. Staff interactions will include phenotyping mutant mice, interpreting experimental results and consulting with staff members for the design and implementation of experiments. In addition, the pathologist will work with the Manager, Necropsy Service to develop and refine standard operating procedures for the Necropsy Service and to provide guidance in necropsy techniques and procedures for the pathology technicians. Provided that grant funding is available to cover this effort, up to 20% of the pathologist's time may be devoted to personal and/or collaborative research. The incumbent will also interact on a routine basis LAHS veterinarians specializing in microbiology, epidemiology, and laboratory animal medicine, and with The Jackson Laboratory's renowned mouse pathologists. This position requires excellent interpersonal and communication skills, a strong work ethic, and the ability to work in a diverse and energetic scientific community. Preference will be given to candidates who are eligible for licensure in the state of Maine. For consideration please send a curriculum vitae, the names and addresses of three professional references and a letter describing your qualifications for the position, and interest in this program to Dr. Peggy Danneman, Sr. Director, LAHS, The Jackson Laboratory, 600 Main St, Bar Harbor, ME 04609. For more than 70 years The Jackson Laboratory, an AAALAC-accredited, non-profit, independent research institution, has been dedicated to advancing human health through basic research in mammalian genetics. The Laboratory supplies resources and information to universities and research laboratories around the world and is a renowned education and training center. We are an equal opportunity employer situated in the small Maine coast town of Bar Harbor, adjacent to Acadia National Park. The surrounding area offers unlimited opportunities for outdoor activities, including hiking, biking, boating, kayaking and fishing. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 14 **************************************** **** CONFIDENTIALITY NOTICE **** This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above email address and delete this email immediately. Thank you. ************************************** From kelly.mcqueeney <@t> bms.com Tue Mar 8 08:04:47 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] immunofluorescence staining in frozen tissue In-Reply-To: <20050308010114.63578.qmail@web52907.mail.yahoo.com> References: <20050308010114.63578.qmail@web52907.mail.yahoo.com> Message-ID: <422DB0FF.4030300@bms.com> I used Ki67 antibody (ab833 at 1:50) from Abcam or H-300 antibosy at 1:200 from Santa Cruz. I fix the tissue in ice-cold acetone for 5 minutes. I found it was very important to permeabilize the tissue with TBS + 0.025% TritonX-100 (follow Abcam protocol) for 5 minutes before blocking. If I deleted this step, the nuclear staining was very weak for either antibody.. Best of luck, Kelly Kelly Yang wrote: >Dear histonetters, > > > >We are working on immunofluorescence staining with two markers, ki67 and DNMT1, in human bladder tissue. I had tried ki67 from Dako and DNMT1 from Imgenex, but none of them give us the good result. > > > >Does anyone have a protocol for staining frozen tissue with Ki67 or DNMT1? Also which antibody are you recommending? > >Thank you for your help in advance. > > > > > > >Kelly Yang >Graduate student >Department of Epidemiology >School of Public Heath >University of California, Los Angeles >310-409-9179 >ext. 57795 >yangyc@ucla.edu >__________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From c.m.vanderloos <@t> amc.uva.nl Tue Mar 8 08:45:42 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] RE: IHC humidity chamber? Message-ID: <251ad79251f236.251f236251ad79@amc.uva.nl> Hi, For the IHC humidity incubation chambers (holding 14 slides) you may contact: PanPath BV in the Netherlands ([1]frans@panpath.nl). Chris van der Loos, PhD Dept. of Pathology Academical Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 fax: +31 20 6960389 e-mail: [2]c.m.vanderloos@amc.uva.nl ----- Original Message ----- From "J m" Date Mon, 07 Mar 2005 08:27:16 -0800 To Histonet@lists.utsouthwestern.edu Subject [Histonet] IHC humidity chamber? salut, Does anyone know of a supplier in Canada for the IHC humidity chambers. I used to have a black plastic one with a clear lid but I don't know where it came from or where i can get another one. I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Regular tissue sections seem to "hold" the solution on the slide better than tissue arrays which are more apt to losing there solutions. I don't like using water repelent pens to circle the arrays but any suggestions would be appreciated. References 1. mailto:frans@panpath.nl 2. mailto:c.m.vanderloos@amc.uva.nl From lamarti2 <@t> gundluth.org Tue Mar 8 08:56:28 2005 From: lamarti2 <@t> gundluth.org (lamarti2@gundluth.org) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] PA Message-ID: The information regarding the $450 fee is accurate. Individuals who had previously contacted the AAPA/ASCP were sent notification with information that included the application fee as $450. Sent by: To: "Histonet" histonet-bounces@lists.utsouth cc: western.edu Subject: [Histonet] PA 03/08/2005 04:32 AM Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Tue Mar 8 09:04:17 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:44 2005 Subject: FW: [Histonet] PA Message-ID: -----Original Message----- From: Charles.Embrey Sent: Tuesday, March 08, 2005 9:04 AM To: 'lpwenk@sbcglobal.net' Subject: RE: [Histonet] PA Peggy, The $450 fee is what the ASCP is charging the current AAPA fellows for certification. We have been told that that will be the exam fee. If the actual exam fee is lower then I know several hundred AAPA fellows that pay $450 this year will be highly upset. The new certificates have been received by several of my fellow PA's and are very nicely done. You can see the fee listed on the ASCP application at: ftp://ftp.ascp.org/Grab/PDFs/BORpdf/APPLICATIONPST.pdf Any new news I get I will pass on to the Histonet as well. Charles Embrey Pathologists' Assistant -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lpwenk@sbcglobal.net Sent: Tuesday, March 08, 2005 4:33 AM To: Histonet Subject: [Histonet] PA Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RizoC <@t> chi.osu.edu Tue Mar 8 09:26:26 2005 From: RizoC <@t> chi.osu.edu (Rizo, Christian) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Pathologists' Assistant Position Opening Message-ID: <979FF5962E234F45B06CF0DB7C1AABB201FEA49A@chi2k3ms01.columbuschildrens.net> Good Day! My name is Christian M. Rizo. I am the Manager of the Anatomic Pathology Lab at Children's Hospital in Columbus Ohio. The reason for this e-mail is that I wanted to inform you that we have an opening for a Pathologists' Assistant at our institution. The position is a full-time, exempt position, dayshift with some weekends on-call. Applicants must be a Master's Degree in biological or allied health sciences graduate with three years of anatomical experience OR Bachelor's Degree in biological or allied health sciences graduate with five years of anatomical experience. Candidates who are certified by the AAPA are preferred. This position is responsible for some of the functions normally performed by the anatomic pathologists. Duties include autopsies, photography and grossing of surgical specimens. Duties also may change depending on workload and as assigned by the Vice Chair of the Anatomic Pathology Department. Applicants must have good customer relations, and technical competencies. Teamwork, dependability and communication are necessary for successful candidates. Children's Hospital Columbus Founded by a determined group of women in 1892, Children's Hospital began as a local charity to serve a dozen very ill children. Throughout the following century, this tiny community-funded mission matured into a health care system that today spans the Midwest as one of its preferred providers of pediatric health care. Columbus Children's today is ranked as one of the nation's ten largest children's hospitals and pediatric research centers. Our hospital campus has nearly 700,000 patient visits every year. Located just minutes from downtown Columbus, Children's Hospital is one of the nation's most progressive and sophisticated health care institutions. Specialty areas include * Surgery * Heart transplant/cardiac care * Cancer * Trauma * Rehabilitation * Dialysis * Bone Marrow Transplant * Neurosciences * Research Children's is also the region's only pediatric Level 1 Trauma Center. With over 1.5-million square feet of interior space, the campus at Children's Hospital is vast. * The main hospital is home to the emergency department (one of the busiest pediatric emergency departments in the nation), surgery suites, interventional radiology and all inpatient units. * In August 2005, a new clinical expansion will be established which includes 14 additional state-of-the-art operating suites (which include intra-operative MRI and robotic technologies), new space for the Children's Heart Center to include catheterization laboratories and an additional 28-bed neonatal intensive care unit as well as integrated clinical laboratory services Thank you for your time. Please forward the e-mail to personnel who you think will benefit from it. Christian M. Rizo MBA, HTL (ASCP) Manager, Anatomic Pathology Childrens Hospital 700 Childrens Drive Columbus, Ohio 43205 614.722.5465 RizoC@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From TrudgeonD <@t> rvh.on.ca Tue Mar 8 09:39:16 2005 From: TrudgeonD <@t> rvh.on.ca (Trudgeon, Debbie) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Giemsa Stain For Helicobacter Message-ID: <7969C47511F68542A97B2A721B8B2D7002296C3C@rvh-mail.rvh.on.ca> What specimen types are other labs running Giemsa stain for Helicobacter pylori? We currently run them daily on all stomach, esophagus and duodenum biopsies. Thanks, Debbie Trudgeon Charge Technologist Histology Royal Victoria Hospital 201 Georgian Drive Barrie, ON L4M 6M2 (705) 728-9090 x6446 trudgeond@rvh.on.ca **** CONFIDENTIALITY NOTICE **** This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above email address and delete this email immediately. Thank you. ************************************** From gcallis <@t> montana.edu Tue Mar 8 09:49:38 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Mast cells staining with TOL In-Reply-To: References: Message-ID: <6.0.0.22.1.20050308084612.01b46dd0@gemini.msu.montana.edu> The Churukian Shenk toluidine blue - a wonderfully simple method never gives you a lot of background staining in other tissues. I am doing to attach it to you privately. I know it works on paraffin sections from animal tissues and is NOT affected by decalcification methods either. Plus charge slides should work perfectly. At 07:31 PM 3/7/2005, you wrote: > >Hi all, > > > >We are looking for the Mast cells in rat brain. I have free floating >tissue sections. The whole brain is fixed in formalin, sucrose and finally >cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know >what would be the most suitable protocol for staining the Mast cells with >Toludine blue. I would also like to know if I could use positively charged >glass slides instead of gelatin coated. Finally, I would like to know the >most stable concentration for Toludine blue stock solution. > >Best regards, > >Amira > >********************************************************************************************************************************** >Amira Fitieh >Research student >Arvid Carlsson Institute for Neuroscience >G?teborg University >Medicinaregata 11 >Box 432 >405 30 G?teborg >*********************************************************************************************************************************** >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From hymclab <@t> hyhc.com Tue Mar 8 10:31:40 2005 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 14 Message-ID: If you mean for H. pylori, we run them on all stomach biopsies that come through. Most of them have the order for H. pylori written on the requisition with the stomach biposy. Dawn -----Original Message----- From: Trudgeon, Debbie [mailto:TrudgeonD@rvh.on.ca] Sent: Tuesday, March 08, 2005 7:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 14 Hi, About 3 weeks ago, I subscribed to Histonet and I submitted a question. I have not heard back or seen anything about it. Does this mean the moderator turned it down? The question was - What specimen sites are other labs running a Giemsa stain on? We currently run them daily on esophagus, stomach, and duodenum. Thanks, Debbie Debbie Trudgeon Charge Technologist Histology Royal Victoria Hospital 201 Georgian Drive Barrie, ON L4M 6M2 (705) 728-9090 x6446 trudgeond@rvh.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 08, 2005 8:39 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: mouse heart morphology (Gayle Callis) 2. mouse heart morphology (David McClister) 3. PDGFR antibody (Katri Tuomala) 4. RE: tried posting this once already... didn't work. (Kristen Broomall) 5. PKA antibody (Caroline Bass) 6. mouse heart morphology (Instrumedics) 7. mouse heart morphology (Stephen Peters M.D.) 8. RE: How people get into histology, and what education (Morken, Tim - Labvision) 9. Re: PDGFR antibody (Kelly D Mcqueeney) 10. Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology (Gayle Callis) 11. Re: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology (Kelly D Mcqueeney) 12. RE: Excellent suggestions on filling heart for better (Re: [Histo net]) mouse heart morphology (Koelling, Ray) 13. RE: workshops & society meeting attendees (awdaf asdfadf) 14. immunofluorescence staining in frozen tissue (Kelly Yang) 15. Job Openings at UConn (alyssa piche) 16. Mast cells staining with TOL (Amira Fitieh) 17. How do I Unsubscribe? (SURGPATH19@aol.com) 18. Re: JACHO Compliance (lpwenk@sbcglobal.net) 19. (no subject) (luckard@verizon.net) 20. RE: IHC humidity chamber? (JOHN PHILLIPS) 21. HT pass rates (lpwenk@sbcglobal.net) 22. PA (lpwenk@sbcglobal.net) 23. hardness of HT exam (lpwenk@sbcglobal.net) 24. Veterinary Pathologist needed (Lesley S. Bechtold) ---------------------------------------------------------------------- Message: 1 Date: Mon, 07 Mar 2005 11:25:04 -0700 From: Gayle Callis Subject: Re: [Histonet] mouse heart morphology To: "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050307105927.01b45ab8@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Stephen, Sorry buy -40C is NOT cold enough for freezing our murine tissues. I believe that others have experienced this problem also, the one of getting freezing artifact/damaged morphology with inside cryostat temperature freezing. Nothing scary about liquid nitrogen usage, although Isopentane is more of a worry for storage. I was thinking more of precooling the metal device containing wells i.e surround the device with liquid nitrogen, then you have the wells at the extremely cold temperatures. Liquid nitrogen in the wells would not be ideal, to be sure. We do this with a metal block sitting in liquid nitrogen so the metal block is at liquid nitrogen temperatures, then place an OCT embedded murine tissue (inside a Tissue Tek plastic mold) on top of the very cold metal block. This permits murine tissue to freeze at a much colder temperature and without artifact. I have seen your device and like its design, but if mouse tissue cannot be snap frozen at colder temperature than -40C, then we can't use it due to terrible morphology. Cryostat freezing temperatures for murine cryomicrotomy simply do not do the job well enough. It is the water ice crystal formation inside the tissue that presents the big problem and the colder the freezing temperature, the smaller the ice crystal formation - hence snap freezing in the true sense of the words. Precooling OCT and a tissue is not feasible when you have 10 mice lined up and collecting 15 tissues out of each mouse as fast as you can dissect and snap freeze plus the actual snap freezing takes only seconds or so - extremely fast. I would love to try your device in the way we use our metal block surround by liquid nitrogen as your device has a very efficient design. There is no doubt it works very well for clinical laboratories for rapid diagnostic work, but for our fussy murine tissues, colder temperatures must prevail. There is an excellent discussion by Charles Scouten on snap freezing biological samples can be found at www.myneurolab.com with details on ice crystal formation, etc. - what we experience with murine tissue. Gayle Callis At 10:52 AM 3/7/2005, you wrote: >HI Gail, > >I have not experimented with liquid nitrogen in my wells. Sounds scary! >In order to reduce freeze artifact using my system I have a few >suggestions. >1) Cool the tissue and OCT to refriderator temp. I think this is a smart >idea for any > snap freezing technique. Why should we add the calories to go from room > temp to >0 degrees C, when it takes a lot less calories to go from 1 degree to O >degrees C. >2) My bars can be cooled down as low as about - 40 C and still maintain >the adhesive quality of the cold metal which is what allows us to be most >precise. Chucks can be put in >liquid nitrogen to get them super cooled. > >I would love to see someone try this and tell me how their result came >out. I think it would freeze pretty quickly. > >Any takers? Got to run for a frozen. > > >Stephen > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 2 Date: Mon, 07 Mar 2005 13:29:42 -0500 From: "David McClister" Subject: [Histonet] mouse heart morphology To: Message-ID: Content-Type: text/plain; charset=US-ASCII Here is some more info about my situation with these mice hearts. Once the heart is extracted, it is put in saline 5 min, blotted dry, longitudinally cut and frozen in a mixture of ethanol and dry ice then stored in -80. The temperature of the crystat is -20. When the heart is cut, I cannot see the RV and LV chambers just one big heart. I'm doing a H&E and taking a 1x image to see the heart. Dentamold has been used in the past to keep the chambers separate but I could not get quality sections cutting through it. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks again, Dave McClister ------------------------------ Message: 3 Date: Mon, 7 Mar 2005 13:41:41 -0500 From: "Katri Tuomala" Subject: [Histonet] PDGFR antibody To: Message-ID: <009e01c52345$4dbf1090$6a9a9618@Katri> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Histonetters, Has anyone used an antibody against PDGFR (platelet derived growth factor receptor) successfully on FFPE (formalin fixed paraffin embedded) human tissue? Could you let me know, which copany's antibody you used. Thank you! Katri Katri Tuomala Hamilton, Ontario, Canada ------------------------------ Message: 4 Date: Mon, 7 Mar 2005 14:08:03 -0500 From: Kristen Broomall Subject: RE: [Histonet] tried posting this once already... didn't work. To: "'TheBestTime23@aol.com'" , histonet@lists.utsouthwestern.edu Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A794E@wlmmsx01.nemours.org> Content-Type: text/plain; charset="iso-8859-1" Ok now.... "As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools." I'm sure someone else has already jumped on this one but, even though the numbers are diminishing, there are still colleges that offer degrees in Histotechnology. I just graduated from one last year and in fact am helping to teach this year's students. Here's a link: http://www.nsh.org/education/schools.html I have a Bachelor of Science and decided to return to school several years later to get an Associate of Science in Histotechnology. I think that my previous schooling only strengthens what I've learned in Histology. I don't know if I would retain half as much knowledge if I didn't already have a science background. I know that getting a 2 year degree after a 4 year degree is a bit backwards, but I'm pleased to tell you that I made a huge career leap forward by becoming a histotech. I've been a histotech for almost a year now & I can tell you that I'm still learning new things everyday and it is really rewarding to do stuff like finally get a ISH protocol right and be able to work out an immuno that just won't work right. I love what I do now and honestly, my 4 year degree helped me to get the histotech position that I have now. Just please don't put down the benefits of a 4 year degree. Knowledge is power. Kristen Broomall, HT (ASCP) -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: Friday, March 04, 2005 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tried posting this once already... didn't work. OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 7 Mar 2005 14:27:28 -0500 From: Caroline Bass Subject: [Histonet] PKA antibody To: " " Message-ID: Content-Type: text/plain; charset=US-ASCII; format=flowed Hello, Could anyone recommend a good PKA antibody? I am looking for something that recognizes the C-alpha subunit and that is useful in mouse brain IHC or tissue culture. Any advice or suggestions would be appreciated. I would particularly like any hands on knowledge of the antibody in mouse tissue or western blot. Thanks, Caroline Bass Beth Israel Deaconess Medical Center ------------------------------ Message: 6 Date: Mon, 7 Mar 2005 14:48:01 -0500 From: "Instrumedics" Subject: [Histonet] mouse heart morphology To: Message-ID: <00fc01c5234e$973d10b0$6401a8c0@INSTRUMEDICS22> Content-Type: text/plain; charset="iso-8859-1" David, If you freeze the heart on the Gentle Jane device and if you use the CryoJane Tape-Transfer process to prepare your frozen sections you can produce paraffin-quality frozen sections. Please visit our web site www.instrumedics.com to see the details. Click on the "gallery" to see many photomicrographs of CryoJane prepared frozen sections. You can request a CD which has a full demonstration of the tape-transfer process from snap-freezing the tissue on the Gentle Jane to fixation of the section We welcome any questions you may have. Bernice Instrumedics 800-237-2772 ----- Original Message ----- From: "David McClister" To: Sent: Monday, March 07, 2005 11:29 AM Subject: [Histonet] mouse heart morphology Hello, I am embedding longitudinal sectioned mice heart in OTC and need to have better morphology to be able to clearly see both left and right ventricles. This lab has tried dentamold but I found it very difficult to section. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks David McClister, HTL (ASCP) Medical University of South Carolina Dept of Cardiothoracic Research 117 Doughty St Charleston, SC 29425 ------------------------------ Message: 7 Date: Mon, 7 Mar 2005 12:06:30 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] mouse heart morphology To: histonet@lists.utsouthwestern.edu Message-ID: <20050307200630.14956.qmail@web30403.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii David, Here is a idea based on no experience: Inject the fresh heart with OCT. Place a landmark with ink or suture to maintain orientation. Freeze it whole. Do either A or B A) Embed the entire heart in the desired orientation and simply trim down until you reach the desired plane of section. B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in the desired plane and embed the halves to achieve the desired plane of section. As far as getting high quality frozens section slides that is quite possible with good technique. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 ------------------------------ Message: 8 Date: Mon, 7 Mar 2005 15:08:32 -0500 From: "Morken, Tim - Labvision" Subject: [Histonet] RE: How people get into histology, and what education To: "histonet (E-mail)" Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CA83@usca0082k08.labvision.apogent.com> Content-Type: text/plain Megan, Probably on the order of 99 percent of histotechs "fall" into the field. I travel around a lot and it is very, very rare to meet someone from the US who went through a formal program (and there are 4-year as well as 2-year programs). This is both a benefit and curse for histotechnology. A benefit because it means literally anyone with very basic biology and chemistry background can get into it, and it draws in a very diverse group of people. A curse because it means that few of those histotechs have the background to become well-versed in the field (as evidenced by the quantity of very basic questions on Histonet). In the past most histotechs did not become certifed by ASCP. I think that is changingnow , but most still only learn what they need for the job at hand. And most people in school never hear about the profession because of the lack of programs. Because of that the field does not draw well from the pool of people that are available for, and would be interested in this kind of work. Of course the current shortage is great for those in the field now - higher pay, pick your job, etc. You're right that for most hisotechnology work a AA degree is fine. In fact, it may be better because there are more openings for bench workers than for supervisory level people. It just depends on your goals. Even the vaunted Genentech biotech company has found that hiring AA-degree people is better for their business because they stay longer. They used to have a policy of only hiring BA/BS at a minimum, but found turnover was way too high for those people (average of two years). AA-degree people, for whatever reason, are more interested in staying in one job longer. It seems there is an annual Histonet discussion of the merits of on-the-job training (OJT) verses academic training. Of course, both are necessary and both contribute to success. We can find examples of both doing better or worse than others in the field. I've seen both groups do very well, and can tell horror stores of labs with the worst of each. The point is that the vast majority of histotechs fall into the position and learn on the job. But, from my own experience in working with may people, in general, those with more academic training are going to have the background to learn new things faster and maybe do better in more advanced technologies. For certain jobs that will be a key advantage. My advice to anyone in the field is to take your job description as a suggestion only and don't be tied down to it. Learn everything there is to do in the lab and take the opportunities as they come. It will be a much more interesting job and can take you to some interesting places! Tim Morken -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: Friday, March 04, 2005 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tried posting this once already... didn't work. OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 07 Mar 2005 15:08:36 -0500 From: Kelly D Mcqueeney Subject: Re: [Histonet] PDGFR antibody To: Katri Tuomala Cc: histonet@lists.utsouthwestern.edu Message-ID: <422CB4C4.2060604@bms.com> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Hi Katria, I have used phospho-PDGFR antibody from Abcam (ab5511) on formalin-fixed paraffin sections of human kidney. You have to incubate O/N at 1:100 dilution and use TBS-tween as a wash buffer. Kelly Katri Tuomala wrote: > Hi Histonetters, > Has anyone used an antibody against PDGFR (platelet derived growth > factor receptor) successfully on FFPE (formalin fixed paraffin > embedded) human tissue? Could you let me know, which copany's antibody > you used. Thank you! > Katri > > Katri Tuomala > Hamilton, Ontario, Canada > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 07 Mar 2005 13:45:27 -0700 From: Gayle Callis Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology To: "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050307133842.01b51640@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Filling the heart with OCT and then snap freezing is an excellent suggestion, and basically the same as filling mouse lungs with OCT to get stable, gently distended alveoli and excellent sectioning quality. When you bisect the heart after OCT fill and snap freeze, a room temperature teflon coated razor blade (EBS of Ted Pella or a teflon coated disposable microtome blade slices through frozen tissue/OCT smoothly, evenly plus the blades are very thin. We slice frozen tissue blocks frequently this way, without tissue damage. Gayle Callis At 01:06 PM 3/7/2005, you wrote: >David, > >Here is a idea based on no experience: > >Inject the fresh heart with OCT. Place a landmark with ink or suture >to >maintain orientation. >Freeze it whole. Do either A or B > >A) Embed the entire heart in the desired orientation and simply trim >down >until you reach the desired plane of section. > >B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it >in >the desired plane and embed the halves to achieve the desired plane of section. > >As far as getting high quality frozens section slides that is quite >possible with good technique. > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 07 Mar 2005 17:08:51 -0500 From: Kelly D Mcqueeney Subject: Re: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology To: Gayle Callis Cc: Histonet@lists.utsouthwestern.edu, "Stephen Peters M.D." Message-ID: <422CD0F3.5010207@bms.com> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 This may sound weird, but has anyone ever perfused a rodent with OCT (or a dilution of OCT) for better quality fresh brain tissue (we want to avoid fixation)? Thanks, Kelly Gayle Callis wrote: > Filling the heart with OCT and then snap freezing is an excellent > suggestion, and basically the same as filling mouse lungs with OCT to > get stable, gently distended alveoli and excellent sectioning quality. > > When you bisect the heart after OCT fill and snap freeze, a room > temperature teflon coated razor blade (EBS of Ted Pella or a teflon > coated disposable microtome blade slices through frozen tissue/OCT > smoothly, evenly plus the blades are very thin. We slice frozen > tissue blocks frequently this way, without tissue damage. > > Gayle Callis > > At 01:06 PM 3/7/2005, you wrote: > >> David, >> >> Here is a idea based on no experience: >> >> Inject the fresh heart with OCT. Place a landmark with ink or suture >> to maintain orientation. >> Freeze it whole. Do either A or B >> >> A) Embed the entire heart in the desired orientation and simply trim >> down until you reach the desired plane of section. >> >> B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut >> it in the desired plane and embed the halves to achieve the desired >> plane of section. >> >> As far as getting high quality frozens section slides that is quite >> possible with good technique. >> >> Stephen >> >> >> Stephen Peters M.D. >> Pathology Innovations, LLC >> 410 Old Mill Lane, >> Wyckoff, NJ 07481 >> 201 847 7600 >> www.pathologyinnovations.com >> >> Senior Attending Pathologist >> Hackensack University Medical Center >> 201 996 4836 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 7 Mar 2005 14:17:32 -0800 From: "Koelling, Ray" Subject: [Histonet] RE: Excellent suggestions on filling heart for better (Re: [Histo net]) mouse heart morphology To: "'Gayle Callis'" , "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <16834C6DFFA6004C88DE4507FB8AE544018CC504@wa-mb4-sea.amgen.com> Content-Type: text/plain; charset="iso-8859-1" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Monday, March 07, 2005 12:45 PM To: Stephen Peters M.D.; Histonet@lists.utsouthwestern.edu Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology Filling the heart with OCT and then snap freezing is an excellent suggestion, and basically the same as filling mouse lungs with OCT to get stable, gently distended alveoli and excellent sectioning quality. When you bisect the heart after OCT fill and snap freeze, a room temperature teflon coated razor blade (EBS of Ted Pella or a teflon coated disposable microtome blade slices through frozen tissue/OCT smoothly, evenly plus the blades are very thin. We slice frozen tissue blocks frequently this way, without tissue damage. Gayle Callis At 01:06 PM 3/7/2005, you wrote: >David, > >Here is a idea based on no experience: > >Inject the fresh heart with OCT. Place a landmark with ink or suture >to >maintain orientation. >Freeze it whole. Do either A or B > >A) Embed the entire heart in the desired orientation and simply trim >down >until you reach the desired plane of section. > >B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it >in >the desired plane and embed the halves to achieve the desired plane of section. > >As far as getting high quality frozens section slides that is quite >possible with good technique. > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 08 Mar 2005 00:51:13 +0000 From: "awdaf asdfadf" Subject: RE: [Histonet] workshops & society meeting attendees To: la.sebree@hosp.wisc.edu, a.schmidt@fuse.net, Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed I agree ! Ventana is excellent about having knowledgeable speakers attend society meetings. Other Companies like Dako are also OK. Both have a high profile and professional presence. I've not heard of Vision Biosystems speaking at too many society meetings. They seem amateurish compared to the other 2. Since their house-cleaning last year, the prospects, from all accounts, are not great. The best sales people were let go to make room for a party group that spends more time socializing than attending to business. How many Companies can one person run into the ground ? Hope to hear Ventana at a future meeting. >From: "Sebree Linda A." >To: , >Subject: RE: [Histonet] Ventana workshops >Date: Fri, 4 Mar 2005 08:28:21 -0600 > >Angela, > >We have found Ventana very receptive to such requests. They are more >than >happy to provide speakers/workshop leaders for state society meetings. So >I suggest you contact them before your next meeting to see if something >can be arranged. I know Ethel Macrea is an excellent, experienced speaker >for just these sort of situations. > >Linda A. Sebree >University of Wisconsin Hospital & Clinics >IHC/ISH Clinical & Research Laboratory >DM223-VA >600 Highland Ave. >Madison, WI 53792 >(608)265-6596 >FAX: (608)262-7174 > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela >M. Schmidt >Sent: Thursday, March 03, 2005 5:18 PM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Ventana workshops > > >Julie, > I think that it would be great if Ventana could put on a basic IHC >and >ISH workshop. We have their technology but understanding the basics in this >growing part of our field would be greatly appreciated!! >Ventana---Southwest OHIO needs you!!! > > > >Angela M. Schmidt HT(ASCP) >Lead Histology Technician >TriHealth Laboratories >Good Samaritan-Bethesda Hospitals >Cincinnati, Ohio >513-872-1508 >a.schmidt@fuse.net > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar - get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ ------------------------------ Message: 14 Date: Mon, 7 Mar 2005 17:01:14 -0800 (PST) From: Kelly Yang Subject: [Histonet] immunofluorescence staining in frozen tissue To: histonet@lists.utsouthwestern.edu Message-ID: <20050308010114.63578.qmail@web52907.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear histonetters, We are working on immunofluorescence staining with two markers, ki67 and DNMT1, in human bladder tissue. I had tried ki67 from Dako and DNMT1 from Imgenex, but none of them give us the good result. Does anyone have a protocol for staining frozen tissue with Ki67 or DNMT1? Also which antibody are you recommending? Thank you for your help in advance. Kelly Yang Graduate student Department of Epidemiology School of Public Heath University of California, Los Angeles 310-409-9179 ext. 57795 yangyc@ucla.edu __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 15 Date: Mon, 07 Mar 2005 20:24:08 -0500 From: "alyssa piche" Subject: [Histonet] Job Openings at UConn To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Hi Everyone, Just wanted to let everyone know that there are two part-time histotech positions at UConn Health Center. Check out their website at www.uchc.edu. Tina _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar - get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ ------------------------------ Message: 16 Date: Tue, 8 Mar 2005 03:31:27 +0100 From: "Amira Fitieh" Subject: [Histonet] Mast cells staining with TOL To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all, We are looking for the Mast cells in rat brain. I have free floating tissue sections. The whole brain is fixed in formalin, sucrose and finally cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know what would be the most suitable protocol for staining the Mast cells with Toludine blue. I would also like to know if I could use positively charged glass slides instead of gelatin coated. Finally, I would like to know the most stable concentration for Toludine blue stock solution. Best regards, Amira **************************************************************************** ****************************************************** Amira Fitieh Research student Arvid Carlsson Institute for Neuroscience G?teborg University Medicinaregata 11 Box 432 405 30 G?teborg **************************************************************************** ******************************************************* ------------------------------ Message: 17 Date: Mon, 7 Mar 2005 22:51:36 EST From: SURGPATH19@aol.com Subject: [Histonet] How do I Unsubscribe? To: histonet@lists.utsouthwestern.edu Message-ID: <1d9.3806d79a.2f5e7b48@aol.com> Content-Type: text/plain; charset="US-ASCII" ------------------------------ Message: 18 Date: Tue, 8 Mar 2005 04:50:15 -0500 From: Subject: Re: [Histonet] JACHO Compliance To: "Scholz, Stephen J." , Message-ID: <007801c523c4$3c48e6e0$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" The quote from 2005 JCAHO regulations for laboratories is as follows: http://www.jcaho.org/accredited+organizations/laboratory+services/npsg/05_np sg_lab.htm "Use at least two patient identifiers (neither to be the patient's location) whenever collecting laboratory samples or administering medications or blood products, and use two identifiers to label sample collection containers in the presence of the patient. Processes are established to maintain samples' identity throughout the pre-analytical, analytical and post-analytical processes." Note that this is "whenever COLLECTING laboratory samples". In two different JCAHO laboratory FAQ: http://www.jcaho.org/accredited+organizations/laboratory+services/npsg/npsq_ faqs_2005.htm "Does the requirement to label all specimens with two identifiers in the presence of the patient apply to specimens collected by outside sources, such as physician offices? Yes. It is the intent of this goal to apply to all laboratory specimens. Laboratories may have limited ability to oversee this portion of the pre-analytical process, however, it is expected that all specimens arrive in the laboratory with a minimum of two identifiers on them. (New 1/18/05)" "Do the two sample identifiers have to be placed on every sample cup, container or aliquot used during the analytical process? No. When possible, laboratories are encouraged to label all aliquots with the two sample identifiers. However, it is impractical to expect this for all test systems due to space limitations on smaller sample cups and containers. As long as the samples' identity is maintained throughout the analytical process, this is acceptable. For example, identity is often maintained through use of an accession number or assigned position numbers on an analyzer. (New 1/18/05)" My interpretation is that this ruling applies to the COLLECTION of specimens in the OR, patients' rooms, doctors' offices. This ruling of needing two identifiers does not apply to the specimen once it is in the lab. However, there has to be a policy about accepting or rejecting specimens if they do not have two identifiers (from same FAQ 2005 page): "Does the Joint Commission require laboratories to reject specimens that arrive in the laboratory without two identifiers? No. While this may be an appropriate response for some situations, rejection and recollection may not always be an acceptable option for samples that are irretrievable, problematic or expensive to recollect (Pap smears), or when rejection will produce a significant treatment delay. The standards have previously required laboratories to establish written guidelines for specimen rejection. Such a policy may include a process for completing the specimen identification when recollection is not a reasonable option. It would be appropriate to include a cautionary statement on the laboratory report indicating the specimen was received in the laboratory without complete identification. (New 1/18/05)" Hope this helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Scholz, Stephen J." To: Sent: Friday, February 25, 2005 12:01 PM Subject: [Histonet] JACHO Compliance Hello all; We are about to have a JACHO inspection and a problem was brought to my attention. It involves using two identifiers on all laboratory specimens. The below paragraph is from JCAHO FAQ web site for laboratory compliance with the National Patient Safety Goals. This particular statement seems to answer the question about use of only one identifying number on a pathology or any lab specimen. Please let me know your interpretation and how your laboratories comply to it. Currently we have only the case number (hand written) on the blocks and only the case number (hand written) on the slides until after staining at which point there are printed labels put on them. Take note that this FAQ was updated in January, 2005. FAQ-We use a label for the initial patient sample that contains only one unique identifier, a number, to identify laboratory specimens. This unique identifier can be traced to multiple patient identifiers. Does this meet the intent of the goal? ANS-No. The intent is to use two separate identifiers on the specimen label. Confidence in an accurate identification improves as the number of identifiers increases, depending upon their uniqueness. A single identifier can be more easily misread, resulting in avoidable errors. Bar coding that includes two or more person-specific identifiers (not room number) will comply with this requirement. (New 1/18/05) What are suggestions to my problem. Stephen J. Scholz HT(ASCP) Histology Coordinator OSF St. Anthony Medical Center Rockford IL Phone: 815-395-5410 Fax: 815-395-5364 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 08 Mar 2005 09:46:49 +0000 From: Subject: [Histonet] (no subject) To: Message-ID: <0ID100D7K1U1HD62@vms048.mailsrvcs.net> Content-Type: text/plain; charset=ISO-8859-1 ------------------------------ Message: 20 Date: Tue, 8 Mar 2005 10:00:52 -0000 From: "JOHN PHILLIPS" Subject: RE: [Histonet] IHC humidity chamber? To: "J m" , Message-ID: <166A1E642B5B644DA694C08FD29D0ADC3E1384@ztroy.new-tr.wales.nhs.uk> Content-Type: text/plain; charset="iso-8859-1" Thermo Shandon supply this type of chamber, holds about 8 to 10 slides,if my memory serves me well - THISTIME!!! John, Wrexham, Wales, UK. -----Original Message----- From: J m [mailto:kosmicdog@hotmail.com] Sent: 07 March 2005 16:27 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC humidity chamber? salut, Does anyone know of a supplier in Canada for the IHC humidity chambers. I used to have a black plastic one with a clear lid but I don't know where it came from or where i can get another one. I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Regular tissue sections seem to "hold" the solution on the slide better than tissue arrays which are more apt to losing there solutions. I don't like using water repelent pens to circle the arrays but any suggestions would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu atynt. Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r rheolwr systemau wybod drwy ddefnyddio'r manylion isod. Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod, felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain Cymru. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau?r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru ar www.newalesnhstrust.org.uk This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager using the details below. The contents of this email represent the views of the individual(s) named above and do not necessarily represent the views of the North East Wales NHS Trust. Please be aware that, under the terms of the Freedom of Information Act 2000, the North East Wales NHS Trust may be required to make public the content of any emails or correspondence received. For futher information on Freedom of Information, please refer to the North East Wales NHS Trust website at www.newalesnhstrust.org.uk For further assistance, please contact system.administrator@new-tr.wales.nhs.uk. ------------------------------ Message: 21 Date: Tue, 8 Mar 2005 05:21:20 -0500 From: Subject: [Histonet] HT pass rates To: "Histonet" Message-ID: <009901c523c8$930d9760$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Hi - My email server has been down, so I'm wading into this discussion late. Hope you all don't mind me putting in some statistics into some of the questions/comments. As for the pass rates of the HT exam: 1. July-Dec. 2004 - not yet published, so I don't know where the 75% failure rate figure is coming from for the "last exam". For Jan-June 2004, the HT pass rate was 49%. (that's those people who passed both the written and practical) 2. Typical pass rates: Usually in the range of 45-55%, from my looking back at the last several years of stats. So the first half of 2004 was in the same range. 3. Look back - in the years that followed the advent of DRG's (1984) and CLIA '88 (with the 1994 deadlines), many people who had been working in the field for many years, were scared that they might need to be certified. So they took the exam "at the last minute", without studying, and the pass rates did dip. If I were a betting person, I would be willing to gamble that, when we get the July-Dec 2004 stats, the pass rate is lower than usual, just based on my past experience with phone calls I received after " DRG and CLIA", and the ones I'm receiving now. People not passing the exam, wanting to know: - what books to buy (didn't look at the booklist they received, nor did they study from a book), - what topics to study (didn't look at the outline they received), - do they really HAVE to know all the chemicals in all the stains, or even, - do they have to know all the histology stains if they only do 6? They just figured, since they had been working in the field of "X" years, they knew it all. Sigh. Yes, some that didn't pass say they did study and tell me the books they studied from. But many didn't study, or didn't study enough, or didn't study the right material. So I try to help them over the phone, in my "free time", whatever that is. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 22 Date: Tue, 8 Mar 2005 05:32:47 -0500 From: Subject: [Histonet] PA To: "Histonet" Message-ID: <00a701c523ca$2c1ea920$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 23 Date: Tue, 8 Mar 2005 06:07:37 -0500 From: Subject: [Histonet] hardness of HT exam To: "Histonet" Message-ID: <00b501c523cf$0a53e580$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" The question was raised - Has the HT (and HTL) exam has gotten harder over the years?. Yes and No, from my point of view. Yes - because there are always new topics. Things that were NOT on my certification exam (many years ago) but that I have to teach now: - AIDS, various hepatitis, PCP, Legionella - recycling - lot more IHC - automated equipment (coverslippers, H&E stainers, special stainers) - new stains - safety - regulations (CLIA, CAP, JCAHO) Every year, I add 1 or 2 new lectures, just to keep up with the changes in our field. But that's the natural progression of any medical field. No - because many of the questions are the same. If it was a good question about GMS/fungus 10 years ago, it is still a good question, and is still being used. Many of the questions in the exam pools have been around for years (decades even). My students talk to me after the exam, and they mention the same questions. No - because all the questions are benchmarked. That means - the ASCP histology exam committee knows the pass rate of the "established" questions. These questions have consistently had the same percent of people pass each time these questions are used. When a new question is used, it's pass rate is measured against "established" questions used on the same exam. If a question is out of it's "normal" range, that question is not counted on that exam, and the exam committee reviews the question, to see why it's pass rate changed. (old technology, typing error, whatever) No - because some of the questions on the exams are "test" questions - in other words, the committee is trying a couple out for the first time, to get statistics as to pass rate. These questions are NOT used in the applicants' pass rate. Some of these questions may be very tough on purpose, on new technology, or poorly written (not intentionally, but realized after re-evaluation based on the pass rate for that question), etc. But there have always been questions being tested for the first time. So this is nothing new. So why the perceptions that the exam is harder in the past year? My opinion - a lot of people are taking the exam to meet the 2005 associate degree deadline. In the years past (not including the 2003/2004 rush to take the exam because of the associate degree deadline), about 600 people per year were attempting the exam with about a 50% pass rate. So about 300 would not pass. In 2004, there were 480 that took the exam the first half of the year, and the numbers for the second have were reported via histonet to be around 1000. That means almost 1500 people took the HT exam last year, or more than double the usual number. And, the usual pass rate for the HT exam over the years is about 50%. So, based on past years, we can expect more numbers of people failing in 2004 (700+) than usually even attempt to take the exam in any other year. (Remember, this is my opinion at this time, and not based on any statistics that have been published.) Plus, Histonet is letting a larger community of people hear from people who have failed, or who work with someone who fails. So many histonetters are hearing the failure stories for the first time. As a program director, I've been hearing from people for years (and years). My opinion is that the failure rate has been constant for the past years. I'd love to hear from other program directors about this. I'll let everyone know the pass rate for July-Dec 2004, when they come out. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 24 Date: Tue, 08 Mar 2005 08:02:00 -0500 From: "Lesley S. Bechtold" Subject: [Histonet] Veterinary Pathologist needed To: histonet@Pathology.swmed.edu Message-ID: <6.2.1.2.2.20050308075837.02c6b2a0@aretha.jax.org> Content-Type: text/plain; charset="us-ascii"; format=flowed Hi Everyone, We are looking for a veterinary pathologist. We're located in Bar Harbor ME and work primarily with mice. The official posting and contact information is below. We're anxious to have our own pathologist on board. Thank you! Lesley Board Certified Veterinary Pathologist The Jackson Laboratory is seeking a board-certified veterinary pathologist with extensive experience in the pathology of laboratory mice to join its Laboratory Animal Health Services (LAHS) department. LAHS provides a variety of veterinary, diagnostic, and quality management services supporting the Research, Resource, and Mouse Service divisions of The Jackson Laboratory facility in Bar Harbor, Maine. The pathologist will provide anatomic pathology support for the internal sick mouse/health surveillance program, will mentor internal pathology trainees and otherwise participate in the educational mission of the Laboratory, and will interact with the Laboratory's scientific staff on a fee-for-service basis. Staff interactions will include phenotyping mutant mice, interpreting experimental results and consulting with staff members for the design and implementation of experiments. In addition, the pathologist will work with the Manager, Necropsy Service to develop and refine standard operating procedures for the Necropsy Service and to provide guidance in necropsy techniques and procedures for the pathology technicians. Provided that grant funding is available to cover this effort, up to 20% of the pathologist's time may be devoted to personal and/or collaborative research. The incumbent will also interact on a routine basis LAHS veterinarians specializing in microbiology, epidemiology, and laboratory animal medicine, and with The Jackson Laboratory's renowned mouse pathologists. This position requires excellent interpersonal and communication skills, a strong work ethic, and the ability to work in a diverse and energetic scientific community. Preference will be given to candidates who are eligible for licensure in the state of Maine. For consideration please send a curriculum vitae, the names and addresses of three professional references and a letter describing your qualifications for the position, and interest in this program to Dr. Peggy Danneman, Sr. Director, LAHS, The Jackson Laboratory, 600 Main St, Bar Harbor, ME 04609. For more than 70 years The Jackson Laboratory, an AAALAC-accredited, non-profit, independent research institution, has been dedicated to advancing human health through basic research in mammalian genetics. The Laboratory supplies resources and information to universities and research laboratories around the world and is a renowned education and training center. We are an equal opportunity employer situated in the small Maine coast town of Bar Harbor, adjacent to Acadia National Park. The surrounding area offers unlimited opportunities for outdoor activities, including hiking, biking, boating, kayaking and fishing. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 14 **************************************** **** CONFIDENTIALITY NOTICE **** This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above email address and delete this email immediately. Thank you. ************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Tue Mar 8 10:36:01 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] cellsmears and cytospin for ihc Message-ID: We do a few markers regularly on cytospins and or smears. The general rule is to fix in 95% alcohol right after spinning, do not let them air-dry. From the alcohol we then transfer to the immuno buffer and go from there. We have had good results with storing the cytospins in the same alcohol for short periods of time, but I wouldn't try it for very long. Good luck. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, March 04, 2005 10:54 AM To: Histonetliste (Histonetliste) Subject: [Histonet] cellsmears and cytospin for ihc Hi histonetters, We perform ihc on cellsmears and cytospins very rarely. Therefore we have no really experience. Can anyone tell me, if there is a general preperation protocol for these specimen, that is best for ihc? Air-drying / fixation in . / storage ? Or does it depend on the antibody or the kind of fluid? Thanks in advance Gudrun Lang Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Tue Mar 8 10:36:10 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] IHC humidity chamber? Message-ID: Kosmicdog, I agree with John from the UK. ThermoShandon, who mentions Canado on the back of their catalog sells just that kind of thing. Dana Settembre University Hospital- UMDNJ Newark, NJ USA >>> JOHN PHILLIPS 3/8/2005 5:00:52 AM >>> Thermo Shandon supply this type of chamber, holds about 8 to 10 slides,if my memory serves me well - THISTIME!!! John, Wrexham, Wales, UK. -----Original Message----- From: J m [mailto:kosmicdog@hotmail.com] Sent: 07 March 2005 16:27 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC humidity chamber? salut, Does anyone know of a supplier in Canada for the IHC humidity chambers. I used to have a black plastic one with a clear lid but I don't know where it came from or where i can get another one. I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Regular tissue sections seem to "hold" the solution on the slide better than tissue arrays which are more apt to losing there solutions. I don't like using water repelent pens to circle the arrays but any suggestions would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu atynt. Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r rheolwr systemau wybod drwy ddefnyddio'r manylion isod. Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod, felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain Cymru. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru ar www.newalesnhstrust.org.uk This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager using the details below. The contents of this email represent the views of the individual(s) named above and do not necessarily represent the views of the North East Wales NHS Trust. Please be aware that, under the terms of the Freedom of Information Act 2000, the North East Wales NHS Trust may be required to make public the content of any emails or correspondence received. For futher information on Freedom of Information, please refer to the North East Wales NHS Trust website at www.newalesnhstrust.org.uk For further assistance, please contact system.administrator@new-tr.wales.nhs.uk. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Tue Mar 8 12:08:19 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] PA In-Reply-To: Message-ID: I went to the ASCP website and it hasn't been updated since November. I called the ASCP BOR and left a voice message, no call was returned. I went to the AAPA website and was instructed to go to the ASCP website. I know all the information is for current fellows, but what about people who want to become eligible? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Tuesday, March 08, 2005 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] PA -----Original Message----- From: Charles.Embrey Sent: Tuesday, March 08, 2005 9:04 AM To: 'lpwenk@sbcglobal.net' Subject: RE: [Histonet] PA Peggy, The $450 fee is what the ASCP is charging the current AAPA fellows for certification. We have been told that that will be the exam fee. If the actual exam fee is lower then I know several hundred AAPA fellows that pay $450 this year will be highly upset. The new certificates have been received by several of my fellow PA's and are very nicely done. You can see the fee listed on the ASCP application at: ftp://ftp.ascp.org/Grab/PDFs/BORpdf/APPLICATIONPST.pdf Any new news I get I will pass on to the Histonet as well. Charles Embrey Pathologists' Assistant -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lpwenk@sbcglobal.net Sent: Tuesday, March 08, 2005 4:33 AM To: Histonet Subject: [Histonet] PA Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jewid <@t> mayo.edu Tue Mar 8 12:17:44 2005 From: jewid <@t> mayo.edu (Jewison, Donna E.) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] technovit 9000 new Message-ID: <94ACA3F800EA6748A320D779148E62000170C136@excsrv67.mayo.edu> Has anyone used the new technovit for embedding bone? Is is easy to cut on a microtome? Also is there a way to deplastize the sections for immuno staining? Thanks Donna Jewison From Charles.Embrey <@t> carle.com Tue Mar 8 12:22:55 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] PA Message-ID: I had an ASCP visitor two weeks ago and she said the whole OJT requirement and route question was still in debate. She had hoped that it would be worked out before the end of March. Cross your fingers but I think they may take longer to decide a course of action. As soon as I hear anything I'll post the info. Chuck -----Original Message----- From: Joe Nocito [mailto:JNocito@Pathreflab.com] Sent: Tuesday, March 08, 2005 12:08 PM To: Charles.Embrey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PA I went to the ASCP website and it hasn't been updated since November. I called the ASCP BOR and left a voice message, no call was returned. I went to the AAPA website and was instructed to go to the ASCP website. I know all the information is for current fellows, but what about people who want to become eligible? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Tuesday, March 08, 2005 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] PA -----Original Message----- From: Charles.Embrey Sent: Tuesday, March 08, 2005 9:04 AM To: 'lpwenk@sbcglobal.net' Subject: RE: [Histonet] PA Peggy, The $450 fee is what the ASCP is charging the current AAPA fellows for certification. We have been told that that will be the exam fee. If the actual exam fee is lower then I know several hundred AAPA fellows that pay $450 this year will be highly upset. The new certificates have been received by several of my fellow PA's and are very nicely done. You can see the fee listed on the ASCP application at: ftp://ftp.ascp.org/Grab/PDFs/BORpdf/APPLICATIONPST.pdf Any new news I get I will pass on to the Histonet as well. Charles Embrey Pathologists' Assistant -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lpwenk@sbcglobal.net Sent: Tuesday, March 08, 2005 4:33 AM To: Histonet Subject: [Histonet] PA Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jewid <@t> mayo.edu Tue Mar 8 12:57:15 2005 From: jewid <@t> mayo.edu (Jewison, Donna E.) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] (no subject) Message-ID: <94ACA3F800EA6748A320D779148E62000170C137@excsrv67.mayo.edu> Has anyone used the new technovit for embedding bone? Is is easy to cut on a microtome? Also is there a way to deplastize the sections for immuno staining? Thanks Donna Jewison From jkiernan <@t> uwo.ca Tue Mar 8 13:08:53 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Mast cells staining with TOL References: Message-ID: <422DF845.5C1D93D8@uwo.ca> To stain the mast cells selectively you need to use a cationic dye (such as toluidine blue) at a low pH such as 1.0. If the pH is higher than about 2.5 you will also see neurons (Nissl) and nuclei of all cells. If the method is done correctly the mast cell granules are metachromatic (red) and the RNA and DNA are stained blue. Often the red metachromasia is more of a purple, however, and in a densely cellular tissue such as brain it can be easy to miss mast cells. In the rat's brain they occur around blood vessels in the thalamus, but not usually in great numbers. An alternative approach is to use alcian blue, a cationic dye that stains the heparin in mast cells but not nucleic acids. It can be used at pH 1 or 2.5, and you can apply a pink counterstain to enhance contrast. The literature of mast cells in the brain goes back quite a long way. See, for example: J. Anat. 121:303-311 (1976) Dev. Neurosci. 4:220-224 (1981) -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Amira Fitieh wrote: > > > Hi all, > > > > We are looking for the Mast cells in rat brain. I have free floating tissue sections. The whole brain is fixed in formalin, sucrose and finally cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know what would be the most suitable protocol for staining the Mast cells with Toludine blue. I would also like to know if I could use positively charged glass slides instead of gelatin coated. Finally, I would like to know the most stable concentration for Toludine blue stock solution. > > Best regards, > > Amira > > ********************************************************************************************************************************** > Amira Fitieh > Research student > Arvid Carlsson Institute for Neuroscience > G?teborg University > Medicinaregata 11 > Box 432 > 405 30 G?teborg > *************************************************************************************************** From gcallis <@t> montana.edu Tue Mar 8 13:45:05 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] People who receive and send replies to Histonet Digest so we see RE: Histonet Digest In-Reply-To: References: Message-ID: <6.0.0.22.1.20050308123537.01c20e40@gemini.msu.montana.edu> Dear all who receive Histonet Digest, Don't send a reply to Histonet Digest - please. It kicks back all the messages to those who do not use the digest format, something they may not want to get again and again. Some of us tend to delete RE: Histonet Digest since there is nothing specific in the subject line. Send your answers to: Histonet@lists.utsouthwestern.edu. AND use the subject line with specific information to alert others you have commentary, answers to questions, or inquiries. This way all those who do not using digest format are not overloaded with uyneeded unwanted ton of daily messages. Thanks, folks Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Tue Mar 8 13:49:16 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] technovit 9000 new In-Reply-To: <94ACA3F800EA6748A320D779148E62000170C136@excsrv67.mayo.edu > References: <94ACA3F800EA6748A320D779148E62000170C136@excsrv67.mayo.edu> Message-ID: <6.0.0.22.1.20050308124528.01b65e50@gemini.msu.montana.edu> Technovit 9000 is a methyl methacrylate kit. To remove MMA from thin microtomed sections, soak sections in 60C xylene X 3 changes, proceed with rehydration. You probably will have to use Neil Hands rather stringent pressure cooker retrievals for IHC. He has a publication in J of Histotechnology a few years back on how he succeeded with retrieval using MMA. You can access the journal for first page/abstract of publication via www.NSH.org click on J of Histotechnology. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From lindas <@t> awesomenet.net Tue Mar 8 15:50:28 2005 From: lindas <@t> awesomenet.net (Linda Davis) Date: Fri Sep 16 15:24:44 2005 Subject: Fw: [Histonet] RE: Histonet Digest, Vol 16, Issue 14 Message-ID: <002601c52428$d8345fd0$760ac942@D6JLZ851> We do giemsa stains on all antral bxs, gastric bxs, stomach bxs and pyloric bxs. Linda Davis ----- Original Message ----- From: "Trudgeon, Debbie" To: Sent: Tuesday, March 08, 2005 7:42 AM Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 14 Hi, About 3 weeks ago, I subscribed to Histonet and I submitted a question. I have not heard back or seen anything about it. Does this mean the moderator turned it down? The question was - What specimen sites are other labs running a Giemsa stain on? We currently run them daily on esophagus, stomach, and duodenum. Thanks, Debbie Debbie Trudgeon Charge Technologist Histology Royal Victoria Hospital 201 Georgian Drive Barrie, ON L4M 6M2 (705) 728-9090 x6446 trudgeond@rvh.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 08, 2005 8:39 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: mouse heart morphology (Gayle Callis) 2. mouse heart morphology (David McClister) 3. PDGFR antibody (Katri Tuomala) 4. RE: tried posting this once already... didn't work. (Kristen Broomall) 5. PKA antibody (Caroline Bass) 6. mouse heart morphology (Instrumedics) 7. mouse heart morphology (Stephen Peters M.D.) 8. RE: How people get into histology, and what education (Morken, Tim - Labvision) 9. Re: PDGFR antibody (Kelly D Mcqueeney) 10. Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology (Gayle Callis) 11. Re: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology (Kelly D Mcqueeney) 12. RE: Excellent suggestions on filling heart for better (Re: [Histo net]) mouse heart morphology (Koelling, Ray) 13. RE: workshops & society meeting attendees (awdaf asdfadf) 14. immunofluorescence staining in frozen tissue (Kelly Yang) 15. Job Openings at UConn (alyssa piche) 16. Mast cells staining with TOL (Amira Fitieh) 17. How do I Unsubscribe? (SURGPATH19@aol.com) 18. Re: JACHO Compliance (lpwenk@sbcglobal.net) 19. (no subject) (luckard@verizon.net) 20. RE: IHC humidity chamber? (JOHN PHILLIPS) 21. HT pass rates (lpwenk@sbcglobal.net) 22. PA (lpwenk@sbcglobal.net) 23. hardness of HT exam (lpwenk@sbcglobal.net) 24. Veterinary Pathologist needed (Lesley S. Bechtold) ---------------------------------------------------------------------- Message: 1 Date: Mon, 07 Mar 2005 11:25:04 -0700 From: Gayle Callis Subject: Re: [Histonet] mouse heart morphology To: "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050307105927.01b45ab8@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Stephen, Sorry buy -40C is NOT cold enough for freezing our murine tissues. I believe that others have experienced this problem also, the one of getting freezing artifact/damaged morphology with inside cryostat temperature freezing. Nothing scary about liquid nitrogen usage, although Isopentane is more of a worry for storage. I was thinking more of precooling the metal device containing wells i.e surround the device with liquid nitrogen, then you have the wells at the extremely cold temperatures. Liquid nitrogen in the wells would not be ideal, to be sure. We do this with a metal block sitting in liquid nitrogen so the metal block is at liquid nitrogen temperatures, then place an OCT embedded murine tissue (inside a Tissue Tek plastic mold) on top of the very cold metal block. This permits murine tissue to freeze at a much colder temperature and without artifact. I have seen your device and like its design, but if mouse tissue cannot be snap frozen at colder temperature than -40C, then we can't use it due to terrible morphology. Cryostat freezing temperatures for murine cryomicrotomy simply do not do the job well enough. It is the water ice crystal formation inside the tissue that presents the big problem and the colder the freezing temperature, the smaller the ice crystal formation - hence snap freezing in the true sense of the words. Precooling OCT and a tissue is not feasible when you have 10 mice lined up and collecting 15 tissues out of each mouse as fast as you can dissect and snap freeze plus the actual snap freezing takes only seconds or so - extremely fast. I would love to try your device in the way we use our metal block surround by liquid nitrogen as your device has a very efficient design. There is no doubt it works very well for clinical laboratories for rapid diagnostic work, but for our fussy murine tissues, colder temperatures must prevail. There is an excellent discussion by Charles Scouten on snap freezing biological samples can be found at www.myneurolab.com with details on ice crystal formation, etc. - what we experience with murine tissue. Gayle Callis At 10:52 AM 3/7/2005, you wrote: >HI Gail, > >I have not experimented with liquid nitrogen in my wells. Sounds scary! >In order to reduce freeze artifact using my system I have a few >suggestions. >1) Cool the tissue and OCT to refriderator temp. I think this is a smart >idea for any > snap freezing technique. Why should we add the calories to go from room > temp to >0 degrees C, when it takes a lot less calories to go from 1 degree to O >degrees C. >2) My bars can be cooled down as low as about - 40 C and still maintain >the adhesive quality of the cold metal which is what allows us to be most >precise. Chucks can be put in >liquid nitrogen to get them super cooled. > >I would love to see someone try this and tell me how their result came >out. I think it would freeze pretty quickly. > >Any takers? Got to run for a frozen. > > >Stephen > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 2 Date: Mon, 07 Mar 2005 13:29:42 -0500 From: "David McClister" Subject: [Histonet] mouse heart morphology To: Message-ID: Content-Type: text/plain; charset=US-ASCII Here is some more info about my situation with these mice hearts. Once the heart is extracted, it is put in saline 5 min, blotted dry, longitudinally cut and frozen in a mixture of ethanol and dry ice then stored in -80. The temperature of the crystat is -20. When the heart is cut, I cannot see the RV and LV chambers just one big heart. I'm doing a H&E and taking a 1x image to see the heart. Dentamold has been used in the past to keep the chambers separate but I could not get quality sections cutting through it. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks again, Dave McClister ------------------------------ Message: 3 Date: Mon, 7 Mar 2005 13:41:41 -0500 From: "Katri Tuomala" Subject: [Histonet] PDGFR antibody To: Message-ID: <009e01c52345$4dbf1090$6a9a9618@Katri> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Histonetters, Has anyone used an antibody against PDGFR (platelet derived growth factor receptor) successfully on FFPE (formalin fixed paraffin embedded) human tissue? Could you let me know, which copany's antibody you used. Thank you! Katri Katri Tuomala Hamilton, Ontario, Canada ------------------------------ Message: 4 Date: Mon, 7 Mar 2005 14:08:03 -0500 From: Kristen Broomall Subject: RE: [Histonet] tried posting this once already... didn't work. To: "'TheBestTime23@aol.com'" , histonet@lists.utsouthwestern.edu Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A794E@wlmmsx01.nemours.org> Content-Type: text/plain; charset="iso-8859-1" Ok now.... "As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools." I'm sure someone else has already jumped on this one but, even though the numbers are diminishing, there are still colleges that offer degrees in Histotechnology. I just graduated from one last year and in fact am helping to teach this year's students. Here's a link: http://www.nsh.org/education/schools.html I have a Bachelor of Science and decided to return to school several years later to get an Associate of Science in Histotechnology. I think that my previous schooling only strengthens what I've learned in Histology. I don't know if I would retain half as much knowledge if I didn't already have a science background. I know that getting a 2 year degree after a 4 year degree is a bit backwards, but I'm pleased to tell you that I made a huge career leap forward by becoming a histotech. I've been a histotech for almost a year now & I can tell you that I'm still learning new things everyday and it is really rewarding to do stuff like finally get a ISH protocol right and be able to work out an immuno that just won't work right. I love what I do now and honestly, my 4 year degree helped me to get the histotech position that I have now. Just please don't put down the benefits of a 4 year degree. Knowledge is power. Kristen Broomall, HT (ASCP) -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: Friday, March 04, 2005 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tried posting this once already... didn't work. OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 7 Mar 2005 14:27:28 -0500 From: Caroline Bass Subject: [Histonet] PKA antibody To: " " Message-ID: Content-Type: text/plain; charset=US-ASCII; format=flowed Hello, Could anyone recommend a good PKA antibody? I am looking for something that recognizes the C-alpha subunit and that is useful in mouse brain IHC or tissue culture. Any advice or suggestions would be appreciated. I would particularly like any hands on knowledge of the antibody in mouse tissue or western blot. Thanks, Caroline Bass Beth Israel Deaconess Medical Center ------------------------------ Message: 6 Date: Mon, 7 Mar 2005 14:48:01 -0500 From: "Instrumedics" Subject: [Histonet] mouse heart morphology To: Message-ID: <00fc01c5234e$973d10b0$6401a8c0@INSTRUMEDICS22> Content-Type: text/plain; charset="iso-8859-1" David, If you freeze the heart on the Gentle Jane device and if you use the CryoJane Tape-Transfer process to prepare your frozen sections you can produce paraffin-quality frozen sections. Please visit our web site www.instrumedics.com to see the details. Click on the "gallery" to see many photomicrographs of CryoJane prepared frozen sections. You can request a CD which has a full demonstration of the tape-transfer process from snap-freezing the tissue on the Gentle Jane to fixation of the section We welcome any questions you may have. Bernice Instrumedics 800-237-2772 ----- Original Message ----- From: "David McClister" To: Sent: Monday, March 07, 2005 11:29 AM Subject: [Histonet] mouse heart morphology Hello, I am embedding longitudinal sectioned mice heart in OTC and need to have better morphology to be able to clearly see both left and right ventricles. This lab has tried dentamold but I found it very difficult to section. Does anyone have any suggestions how I can make the morphology of frozen tissue comparable to paraffin embedded tissue? Thanks David McClister, HTL (ASCP) Medical University of South Carolina Dept of Cardiothoracic Research 117 Doughty St Charleston, SC 29425 ------------------------------ Message: 7 Date: Mon, 7 Mar 2005 12:06:30 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] mouse heart morphology To: histonet@lists.utsouthwestern.edu Message-ID: <20050307200630.14956.qmail@web30403.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii David, Here is a idea based on no experience: Inject the fresh heart with OCT. Place a landmark with ink or suture to maintain orientation. Freeze it whole. Do either A or B A) Embed the entire heart in the desired orientation and simply trim down until you reach the desired plane of section. B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in the desired plane and embed the halves to achieve the desired plane of section. As far as getting high quality frozens section slides that is quite possible with good technique. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 ------------------------------ Message: 8 Date: Mon, 7 Mar 2005 15:08:32 -0500 From: "Morken, Tim - Labvision" Subject: [Histonet] RE: How people get into histology, and what education To: "histonet (E-mail)" Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CA83@usca0082k08.labvision.apogent.com> Content-Type: text/plain Megan, Probably on the order of 99 percent of histotechs "fall" into the field. I travel around a lot and it is very, very rare to meet someone from the US who went through a formal program (and there are 4-year as well as 2-year programs). This is both a benefit and curse for histotechnology. A benefit because it means literally anyone with very basic biology and chemistry background can get into it, and it draws in a very diverse group of people. A curse because it means that few of those histotechs have the background to become well-versed in the field (as evidenced by the quantity of very basic questions on Histonet). In the past most histotechs did not become certifed by ASCP. I think that is changingnow , but most still only learn what they need for the job at hand. And most people in school never hear about the profession because of the lack of programs. Because of that the field does not draw well from the pool of people that are available for, and would be interested in this kind of work. Of course the current shortage is great for those in the field now - higher pay, pick your job, etc. You're right that for most hisotechnology work a AA degree is fine. In fact, it may be better because there are more openings for bench workers than for supervisory level people. It just depends on your goals. Even the vaunted Genentech biotech company has found that hiring AA-degree people is better for their business because they stay longer. They used to have a policy of only hiring BA/BS at a minimum, but found turnover was way too high for those people (average of two years). AA-degree people, for whatever reason, are more interested in staying in one job longer. It seems there is an annual Histonet discussion of the merits of on-the-job training (OJT) verses academic training. Of course, both are necessary and both contribute to success. We can find examples of both doing better or worse than others in the field. I've seen both groups do very well, and can tell horror stores of labs with the worst of each. The point is that the vast majority of histotechs fall into the position and learn on the job. But, from my own experience in working with may people, in general, those with more academic training are going to have the background to learn new things faster and maybe do better in more advanced technologies. For certain jobs that will be a key advantage. My advice to anyone in the field is to take your job description as a suggestion only and don't be tied down to it. Learn everything there is to do in the lab and take the opportunities as they come. It will be a much more interesting job and can take you to some interesting places! Tim Morken -----Original Message----- From: TheBestTime23@aol.com [mailto:TheBestTime23@aol.com] Sent: Friday, March 04, 2005 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tried posting this once already... didn't work. OK. I really wasn't expecting much of a response from my post, and was really surprised by what I got. I want to start by apologizing to all that I have offended. It surely wasn't my intent. And while I have thought of a lot of things to say in response to your many e-mails, I will try to keep this within reason. First: I like my job. I feel very fortunate to have gotten into a field that pays me well, keeps me interested and has a lot of potential for growth. I take pride in the work that I do and I love learning new things every day. I have recently gotten the opportunity to train on immunos and I couldn't be more thrilled. Second, and probably more to the point: I was really trying to make a comment about the requirements for being a histotech, not a statement about the job itself. The e-mail I was responding to had mentioned 4 years of college as a pre-req, and I thought that was an excessive amount. I probably have a skewed point of view, having dropped out of college after only one semester, but I think that there are many bright and talented people that haven't gone to college that could still do wonderfully as histotechs. If you had 4 years of college as a requirement and add another 2 to learn the histo stuff, you're looking at 6 years. You could become a pathology assistant in that amount of time and be earning a whole lot more when you were done and still be working in a similar field. That was my only real point. I understand that they want people to have more education and that's fine. I like the way that the ASCP also takes credit hours into consideration and is not just looking for a degree. But 4 years, in my opinion, is too much. WAY too much. I would hate to think how many very talented histotechs we would not have now had the requirements been that stiff 20 years ago. I guess my third point is more of a question. I know how I got to be a histotech. I basically fell into it. I knew someone who worked in a lab and I started as a lab aid, heard about on the job training and went from there. I know a lot of people who started that way, or as phlebotomists or something similar. How many people got started in a similar way? I also know that most people get a totally blank look on their face when you tell them that you work in histology. I had certainly never heard of it before. How many of you had? I can't see many people looking through a course list and saying to themselves, "oh, histology, that would be perfect for me", because most of them wouldn't know what the heck it was. As far as I know (and this is mostly a guess) there aren't any 2 year programs at tech schools or anything like that. Histology is kind of an anomaly that way. Taught in hospitals and clinics, but not schools. Maybe the on the job training wasn't such a bad thing. At least it would get those remaining empty spots full, until some more concrete method of teaching our craft is set up. Just another thought. One that I hope won't get me into any more trouble : ) My apologies, Megan Grateful new histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 07 Mar 2005 15:08:36 -0500 From: Kelly D Mcqueeney Subject: Re: [Histonet] PDGFR antibody To: Katri Tuomala Cc: histonet@lists.utsouthwestern.edu Message-ID: <422CB4C4.2060604@bms.com> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Hi Katria, I have used phospho-PDGFR antibody from Abcam (ab5511) on formalin-fixed paraffin sections of human kidney. You have to incubate O/N at 1:100 dilution and use TBS-tween as a wash buffer. Kelly Katri Tuomala wrote: > Hi Histonetters, > Has anyone used an antibody against PDGFR (platelet derived growth > factor receptor) successfully on FFPE (formalin fixed paraffin > embedded) human tissue? Could you let me know, which copany's antibody > you used. Thank you! > Katri > > Katri Tuomala > Hamilton, Ontario, Canada > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 07 Mar 2005 13:45:27 -0700 From: Gayle Callis Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology To: "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050307133842.01b51640@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Filling the heart with OCT and then snap freezing is an excellent suggestion, and basically the same as filling mouse lungs with OCT to get stable, gently distended alveoli and excellent sectioning quality. When you bisect the heart after OCT fill and snap freeze, a room temperature teflon coated razor blade (EBS of Ted Pella or a teflon coated disposable microtome blade slices through frozen tissue/OCT smoothly, evenly plus the blades are very thin. We slice frozen tissue blocks frequently this way, without tissue damage. Gayle Callis At 01:06 PM 3/7/2005, you wrote: >David, > >Here is a idea based on no experience: > >Inject the fresh heart with OCT. Place a landmark with ink or suture to >maintain orientation. >Freeze it whole. Do either A or B > >A) Embed the entire heart in the desired orientation and simply trim down >until you reach the desired plane of section. > >B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in >the desired plane and embed the halves to achieve the desired plane of >section. > >As far as getting high quality frozens section slides that is quite >possible with good technique. > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 07 Mar 2005 17:08:51 -0500 From: Kelly D Mcqueeney Subject: Re: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology To: Gayle Callis Cc: Histonet@lists.utsouthwestern.edu, "Stephen Peters M.D." Message-ID: <422CD0F3.5010207@bms.com> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 This may sound weird, but has anyone ever perfused a rodent with OCT (or a dilution of OCT) for better quality fresh brain tissue (we want to avoid fixation)? Thanks, Kelly Gayle Callis wrote: > Filling the heart with OCT and then snap freezing is an excellent > suggestion, and basically the same as filling mouse lungs with OCT to > get stable, gently distended alveoli and excellent sectioning quality. > > When you bisect the heart after OCT fill and snap freeze, a room > temperature teflon coated razor blade (EBS of Ted Pella or a teflon > coated disposable microtome blade slices through frozen tissue/OCT > smoothly, evenly plus the blades are very thin. We slice frozen > tissue blocks frequently this way, without tissue damage. > > Gayle Callis > > At 01:06 PM 3/7/2005, you wrote: > >> David, >> >> Here is a idea based on no experience: >> >> Inject the fresh heart with OCT. Place a landmark with ink or suture >> to maintain orientation. >> Freeze it whole. Do either A or B >> >> A) Embed the entire heart in the desired orientation and simply trim >> down until you reach the desired plane of section. >> >> B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut >> it in the desired plane and embed the halves to achieve the desired >> plane of section. >> >> As far as getting high quality frozens section slides that is quite >> possible with good technique. >> >> Stephen >> >> >> Stephen Peters M.D. >> Pathology Innovations, LLC >> 410 Old Mill Lane, >> Wyckoff, NJ 07481 >> 201 847 7600 >> www.pathologyinnovations.com >> >> Senior Attending Pathologist >> Hackensack University Medical Center >> 201 996 4836 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 7 Mar 2005 14:17:32 -0800 From: "Koelling, Ray" Subject: [Histonet] RE: Excellent suggestions on filling heart for better (Re: [Histo net]) mouse heart morphology To: "'Gayle Callis'" , "Stephen Peters M.D." , Histonet@lists.utsouthwestern.edu Message-ID: <16834C6DFFA6004C88DE4507FB8AE544018CC504@wa-mb4-sea.amgen.com> Content-Type: text/plain; charset="iso-8859-1" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Monday, March 07, 2005 12:45 PM To: Stephen Peters M.D.; Histonet@lists.utsouthwestern.edu Subject: Excellent suggestions on filling heart for better (Re: [Histonet]) mouse heart morphology Filling the heart with OCT and then snap freezing is an excellent suggestion, and basically the same as filling mouse lungs with OCT to get stable, gently distended alveoli and excellent sectioning quality. When you bisect the heart after OCT fill and snap freeze, a room temperature teflon coated razor blade (EBS of Ted Pella or a teflon coated disposable microtome blade slices through frozen tissue/OCT smoothly, evenly plus the blades are very thin. We slice frozen tissue blocks frequently this way, without tissue damage. Gayle Callis At 01:06 PM 3/7/2005, you wrote: >David, > >Here is a idea based on no experience: > >Inject the fresh heart with OCT. Place a landmark with ink or suture to >maintain orientation. >Freeze it whole. Do either A or B > >A) Embed the entire heart in the desired orientation and simply trim down >until you reach the desired plane of section. > >B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in >the desired plane and embed the halves to achieve the desired plane of section. > >As far as getting high quality frozens section slides that is quite >possible with good technique. > >Stephen > > >Stephen Peters M.D. >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > >Senior Attending Pathologist >Hackensack University Medical Center >201 996 4836 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 08 Mar 2005 00:51:13 +0000 From: "awdaf asdfadf" Subject: RE: [Histonet] workshops & society meeting attendees To: la.sebree@hosp.wisc.edu, a.schmidt@fuse.net, Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed I agree ! Ventana is excellent about having knowledgeable speakers attend society meetings. Other Companies like Dako are also OK. Both have a high profile and professional presence. I've not heard of Vision Biosystems speaking at too many society meetings. They seem amateurish compared to the other 2. Since their house-cleaning last year, the prospects, from all accounts, are not great. The best sales people were let go to make room for a party group that spends more time socializing than attending to business. How many Companies can one person run into the ground ? Hope to hear Ventana at a future meeting. >From: "Sebree Linda A." >To: , >Subject: RE: [Histonet] Ventana workshops >Date: Fri, 4 Mar 2005 08:28:21 -0600 > >Angela, > >We have found Ventana very receptive to such requests. They are more than >happy to provide speakers/workshop leaders for state society meetings. So >I suggest you contact them before your next meeting to see if something >can be arranged. I know Ethel Macrea is an excellent, experienced speaker >for just these sort of situations. > >Linda A. Sebree >University of Wisconsin Hospital & Clinics >IHC/ISH Clinical & Research Laboratory >DM223-VA >600 Highland Ave. >Madison, WI 53792 >(608)265-6596 >FAX: (608)262-7174 > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela M. >Schmidt >Sent: Thursday, March 03, 2005 5:18 PM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Ventana workshops > > >Julie, > I think that it would be great if Ventana could put on a basic IHC and >ISH workshop. We have their technology but understanding the basics in this >growing part of our field would be greatly appreciated!! >Ventana---Southwest OHIO needs you!!! > > > >Angela M. Schmidt HT(ASCP) >Lead Histology Technician >TriHealth Laboratories >Good Samaritan-Bethesda Hospitals >Cincinnati, Ohio >513-872-1508 >a.schmidt@fuse.net > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar - get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ ------------------------------ Message: 14 Date: Mon, 7 Mar 2005 17:01:14 -0800 (PST) From: Kelly Yang Subject: [Histonet] immunofluorescence staining in frozen tissue To: histonet@lists.utsouthwestern.edu Message-ID: <20050308010114.63578.qmail@web52907.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear histonetters, We are working on immunofluorescence staining with two markers, ki67 and DNMT1, in human bladder tissue. I had tried ki67 from Dako and DNMT1 from Imgenex, but none of them give us the good result. Does anyone have a protocol for staining frozen tissue with Ki67 or DNMT1? Also which antibody are you recommending? Thank you for your help in advance. Kelly Yang Graduate student Department of Epidemiology School of Public Heath University of California, Los Angeles 310-409-9179 ext. 57795 yangyc@ucla.edu __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 15 Date: Mon, 07 Mar 2005 20:24:08 -0500 From: "alyssa piche" Subject: [Histonet] Job Openings at UConn To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Hi Everyone, Just wanted to let everyone know that there are two part-time histotech positions at UConn Health Center. Check out their website at www.uchc.edu. Tina _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar - get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ ------------------------------ Message: 16 Date: Tue, 8 Mar 2005 03:31:27 +0100 From: "Amira Fitieh" Subject: [Histonet] Mast cells staining with TOL To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all, We are looking for the Mast cells in rat brain. I have free floating tissue sections. The whole brain is fixed in formalin, sucrose and finally cryo-preserved in glycerol, ethylene glycol and PBS. I would like to know what would be the most suitable protocol for staining the Mast cells with Toludine blue. I would also like to know if I could use positively charged glass slides instead of gelatin coated. Finally, I would like to know the most stable concentration for Toludine blue stock solution. Best regards, Amira ********************************************************************************************************************************** Amira Fitieh Research student Arvid Carlsson Institute for Neuroscience G?teborg University Medicinaregata 11 Box 432 405 30 G?teborg *********************************************************************************************************************************** ------------------------------ Message: 17 Date: Mon, 7 Mar 2005 22:51:36 EST From: SURGPATH19@aol.com Subject: [Histonet] How do I Unsubscribe? To: histonet@lists.utsouthwestern.edu Message-ID: <1d9.3806d79a.2f5e7b48@aol.com> Content-Type: text/plain; charset="US-ASCII" ------------------------------ Message: 18 Date: Tue, 8 Mar 2005 04:50:15 -0500 From: Subject: Re: [Histonet] JACHO Compliance To: "Scholz, Stephen J." , Message-ID: <007801c523c4$3c48e6e0$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" The quote from 2005 JCAHO regulations for laboratories is as follows: http://www.jcaho.org/accredited+organizations/laboratory+services/npsg/05_np sg_lab.htm "Use at least two patient identifiers (neither to be the patient's location) whenever collecting laboratory samples or administering medications or blood products, and use two identifiers to label sample collection containers in the presence of the patient. Processes are established to maintain samples' identity throughout the pre-analytical, analytical and post-analytical processes." Note that this is "whenever COLLECTING laboratory samples". In two different JCAHO laboratory FAQ: http://www.jcaho.org/accredited+organizations/laboratory+services/npsg/npsq_ faqs_2005.htm "Does the requirement to label all specimens with two identifiers in the presence of the patient apply to specimens collected by outside sources, such as physician offices? Yes. It is the intent of this goal to apply to all laboratory specimens. Laboratories may have limited ability to oversee this portion of the pre-analytical process, however, it is expected that all specimens arrive in the laboratory with a minimum of two identifiers on them. (New 1/18/05)" "Do the two sample identifiers have to be placed on every sample cup, container or aliquot used during the analytical process? No. When possible, laboratories are encouraged to label all aliquots with the two sample identifiers. However, it is impractical to expect this for all test systems due to space limitations on smaller sample cups and containers. As long as the samples' identity is maintained throughout the analytical process, this is acceptable. For example, identity is often maintained through use of an accession number or assigned position numbers on an analyzer. (New 1/18/05)" My interpretation is that this ruling applies to the COLLECTION of specimens in the OR, patients' rooms, doctors' offices. This ruling of needing two identifiers does not apply to the specimen once it is in the lab. However, there has to be a policy about accepting or rejecting specimens if they do not have two identifiers (from same FAQ 2005 page): "Does the Joint Commission require laboratories to reject specimens that arrive in the laboratory without two identifiers? No. While this may be an appropriate response for some situations, rejection and recollection may not always be an acceptable option for samples that are irretrievable, problematic or expensive to recollect (Pap smears), or when rejection will produce a significant treatment delay. The standards have previously required laboratories to establish written guidelines for specimen rejection. Such a policy may include a process for completing the specimen identification when recollection is not a reasonable option. It would be appropriate to include a cautionary statement on the laboratory report indicating the specimen was received in the laboratory without complete identification. (New 1/18/05)" Hope this helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Scholz, Stephen J." To: Sent: Friday, February 25, 2005 12:01 PM Subject: [Histonet] JACHO Compliance Hello all; We are about to have a JACHO inspection and a problem was brought to my attention. It involves using two identifiers on all laboratory specimens. The below paragraph is from JCAHO FAQ web site for laboratory compliance with the National Patient Safety Goals. This particular statement seems to answer the question about use of only one identifying number on a pathology or any lab specimen. Please let me know your interpretation and how your laboratories comply to it. Currently we have only the case number (hand written) on the blocks and only the case number (hand written) on the slides until after staining at which point there are printed labels put on them. Take note that this FAQ was updated in January, 2005. FAQ-We use a label for the initial patient sample that contains only one unique identifier, a number, to identify laboratory specimens. This unique identifier can be traced to multiple patient identifiers. Does this meet the intent of the goal? ANS-No. The intent is to use two separate identifiers on the specimen label. Confidence in an accurate identification improves as the number of identifiers increases, depending upon their uniqueness. A single identifier can be more easily misread, resulting in avoidable errors. Bar coding that includes two or more person-specific identifiers (not room number) will comply with this requirement. (New 1/18/05) What are suggestions to my problem. Stephen J. Scholz HT(ASCP) Histology Coordinator OSF St. Anthony Medical Center Rockford IL Phone: 815-395-5410 Fax: 815-395-5364 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 08 Mar 2005 09:46:49 +0000 From: Subject: [Histonet] (no subject) To: Message-ID: <0ID100D7K1U1HD62@vms048.mailsrvcs.net> Content-Type: text/plain; charset=ISO-8859-1 ------------------------------ Message: 20 Date: Tue, 8 Mar 2005 10:00:52 -0000 From: "JOHN PHILLIPS" Subject: RE: [Histonet] IHC humidity chamber? To: "J m" , Message-ID: <166A1E642B5B644DA694C08FD29D0ADC3E1384@ztroy.new-tr.wales.nhs.uk> Content-Type: text/plain; charset="iso-8859-1" Thermo Shandon supply this type of chamber, holds about 8 to 10 slides,if my memory serves me well - THISTIME!!! John, Wrexham, Wales, UK. -----Original Message----- From: J m [mailto:kosmicdog@hotmail.com] Sent: 07 March 2005 16:27 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC humidity chamber? salut, Does anyone know of a supplier in Canada for the IHC humidity chambers. I used to have a black plastic one with a clear lid but I don't know where it came from or where i can get another one. I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Regular tissue sections seem to "hold" the solution on the slide better than tissue arrays which are more apt to losing there solutions. I don't like using water repelent pens to circle the arrays but any suggestions would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu atynt. Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r rheolwr systemau wybod drwy ddefnyddio'r manylion isod. Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod, felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain Cymru. Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau?r Ddeddf Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth, cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru ar www.newalesnhstrust.org.uk This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager using the details below. The contents of this email represent the views of the individual(s) named above and do not necessarily represent the views of the North East Wales NHS Trust. Please be aware that, under the terms of the Freedom of Information Act 2000, the North East Wales NHS Trust may be required to make public the content of any emails or correspondence received. For futher information on Freedom of Information, please refer to the North East Wales NHS Trust website at www.newalesnhstrust.org.uk For further assistance, please contact system.administrator@new-tr.wales.nhs.uk. ------------------------------ Message: 21 Date: Tue, 8 Mar 2005 05:21:20 -0500 From: Subject: [Histonet] HT pass rates To: "Histonet" Message-ID: <009901c523c8$930d9760$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Hi - My email server has been down, so I'm wading into this discussion late. Hope you all don't mind me putting in some statistics into some of the questions/comments. As for the pass rates of the HT exam: 1. July-Dec. 2004 - not yet published, so I don't know where the 75% failure rate figure is coming from for the "last exam". For Jan-June 2004, the HT pass rate was 49%. (that's those people who passed both the written and practical) 2. Typical pass rates: Usually in the range of 45-55%, from my looking back at the last several years of stats. So the first half of 2004 was in the same range. 3. Look back - in the years that followed the advent of DRG's (1984) and CLIA '88 (with the 1994 deadlines), many people who had been working in the field for many years, were scared that they might need to be certified. So they took the exam "at the last minute", without studying, and the pass rates did dip. If I were a betting person, I would be willing to gamble that, when we get the July-Dec 2004 stats, the pass rate is lower than usual, just based on my past experience with phone calls I received after " DRG and CLIA", and the ones I'm receiving now. People not passing the exam, wanting to know: - what books to buy (didn't look at the booklist they received, nor did they study from a book), - what topics to study (didn't look at the outline they received), - do they really HAVE to know all the chemicals in all the stains, or even, - do they have to know all the histology stains if they only do 6? They just figured, since they had been working in the field of "X" years, they knew it all. Sigh. Yes, some that didn't pass say they did study and tell me the books they studied from. But many didn't study, or didn't study enough, or didn't study the right material. So I try to help them over the phone, in my "free time", whatever that is. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 22 Date: Tue, 8 Mar 2005 05:32:47 -0500 From: Subject: [Histonet] PA To: "Histonet" Message-ID: <00a701c523ca$2c1ea920$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 23 Date: Tue, 8 Mar 2005 06:07:37 -0500 From: Subject: [Histonet] hardness of HT exam To: "Histonet" Message-ID: <00b501c523cf$0a53e580$1e2ad445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" The question was raised - Has the HT (and HTL) exam has gotten harder over the years?. Yes and No, from my point of view. Yes - because there are always new topics. Things that were NOT on my certification exam (many years ago) but that I have to teach now: - AIDS, various hepatitis, PCP, Legionella - recycling - lot more IHC - automated equipment (coverslippers, H&E stainers, special stainers) - new stains - safety - regulations (CLIA, CAP, JCAHO) Every year, I add 1 or 2 new lectures, just to keep up with the changes in our field. But that's the natural progression of any medical field. No - because many of the questions are the same. If it was a good question about GMS/fungus 10 years ago, it is still a good question, and is still being used. Many of the questions in the exam pools have been around for years (decades even). My students talk to me after the exam, and they mention the same questions. No - because all the questions are benchmarked. That means - the ASCP histology exam committee knows the pass rate of the "established" questions. These questions have consistently had the same percent of people pass each time these questions are used. When a new question is used, it's pass rate is measured against "established" questions used on the same exam. If a question is out of it's "normal" range, that question is not counted on that exam, and the exam committee reviews the question, to see why it's pass rate changed. (old technology, typing error, whatever) No - because some of the questions on the exams are "test" questions - in other words, the committee is trying a couple out for the first time, to get statistics as to pass rate. These questions are NOT used in the applicants' pass rate. Some of these questions may be very tough on purpose, on new technology, or poorly written (not intentionally, but realized after re-evaluation based on the pass rate for that question), etc. But there have always been questions being tested for the first time. So this is nothing new. So why the perceptions that the exam is harder in the past year? My opinion - a lot of people are taking the exam to meet the 2005 associate degree deadline. In the years past (not including the 2003/2004 rush to take the exam because of the associate degree deadline), about 600 people per year were attempting the exam with about a 50% pass rate. So about 300 would not pass. In 2004, there were 480 that took the exam the first half of the year, and the numbers for the second have were reported via histonet to be around 1000. That means almost 1500 people took the HT exam last year, or more than double the usual number. And, the usual pass rate for the HT exam over the years is about 50%. So, based on past years, we can expect more numbers of people failing in 2004 (700+) than usually even attempt to take the exam in any other year. (Remember, this is my opinion at this time, and not based on any statistics that have been published.) Plus, Histonet is letting a larger community of people hear from people who have failed, or who work with someone who fails. So many histonetters are hearing the failure stories for the first time. As a program director, I've been hearing from people for years (and years). My opinion is that the failure rate has been constant for the past years. I'd love to hear from other program directors about this. I'll let everyone know the pass rate for July-Dec 2004, when they come out. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 24 Date: Tue, 08 Mar 2005 08:02:00 -0500 From: "Lesley S. Bechtold" Subject: [Histonet] Veterinary Pathologist needed To: histonet@Pathology.swmed.edu Message-ID: <6.2.1.2.2.20050308075837.02c6b2a0@aretha.jax.org> Content-Type: text/plain; charset="us-ascii"; format=flowed Hi Everyone, We are looking for a veterinary pathologist. We're located in Bar Harbor ME and work primarily with mice. The official posting and contact information is below. We're anxious to have our own pathologist on board. Thank you! Lesley Board Certified Veterinary Pathologist The Jackson Laboratory is seeking a board-certified veterinary pathologist with extensive experience in the pathology of laboratory mice to join its Laboratory Animal Health Services (LAHS) department. LAHS provides a variety of veterinary, diagnostic, and quality management services supporting the Research, Resource, and Mouse Service divisions of The Jackson Laboratory facility in Bar Harbor, Maine. The pathologist will provide anatomic pathology support for the internal sick mouse/health surveillance program, will mentor internal pathology trainees and otherwise participate in the educational mission of the Laboratory, and will interact with the Laboratory's scientific staff on a fee-for-service basis. Staff interactions will include phenotyping mutant mice, interpreting experimental results and consulting with staff members for the design and implementation of experiments. In addition, the pathologist will work with the Manager, Necropsy Service to develop and refine standard operating procedures for the Necropsy Service and to provide guidance in necropsy techniques and procedures for the pathology technicians. Provided that grant funding is available to cover this effort, up to 20% of the pathologist's time may be devoted to personal and/or collaborative research. The incumbent will also interact on a routine basis LAHS veterinarians specializing in microbiology, epidemiology, and laboratory animal medicine, and with The Jackson Laboratory's renowned mouse pathologists. This position requires excellent interpersonal and communication skills, a strong work ethic, and the ability to work in a diverse and energetic scientific community. Preference will be given to candidates who are eligible for licensure in the state of Maine. For consideration please send a curriculum vitae, the names and addresses of three professional references and a letter describing your qualifications for the position, and interest in this program to Dr. Peggy Danneman, Sr. Director, LAHS, The Jackson Laboratory, 600 Main St, Bar Harbor, ME 04609. For more than 70 years The Jackson Laboratory, an AAALAC-accredited, non-profit, independent research institution, has been dedicated to advancing human health through basic research in mammalian genetics. The Laboratory supplies resources and information to universities and research laboratories around the world and is a renowned education and training center. We are an equal opportunity employer situated in the small Maine coast town of Bar Harbor, adjacent to Acadia National Park. The surrounding area offers unlimited opportunities for outdoor activities, including hiking, biking, boating, kayaking and fishing. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 14 **************************************** **** CONFIDENTIALITY NOTICE **** This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above email address and delete this email immediately. Thank you. ************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Anti-Virus. Version: 7.0.300 / Virus Database: 266.6.4 - Release Date: 3/7/2005 -- No virus found in this outgoing message. Checked by AVG Anti-Virus. Version: 7.0.300 / Virus Database: 266.6.4 - Release Date: 3/7/2005 From bradeupland <@t> yahoo.com Tue Mar 8 20:33:15 2005 From: bradeupland <@t> yahoo.com (brade upland) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] EGFR in FFPE mouse kidney Message-ID: <20050309023315.76330.qmail@web90002.mail.scd.yahoo.com> Hi Histonetters, I have been really struggling trying to get EGFR staining in the tubles of mouse kidneys. I hope that somewhere out there has had good success staining EGFR in mouse kidneys. I know that there are a lot of antibodies out there.....but I have a feeling some or alot of you out there already know the answere. Thank you soo much for your help.......brade --------------------------------- Celebrate Yahoo!'s 10th Birthday! Yahoo! Netrospective: 100 Moments of the Web From arvind <@t> nbrc.res.in Wed Mar 9 01:46:48 2005 From: arvind <@t> nbrc.res.in (Arvind Pundir) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 16 Message-ID: <7DE0291A47C4BC4D9CDEF65D5017CEF0A720@mail.nbrc.res.in> Can anyone tell me how to embed the soft tissue like human fetus brain 5 month old, and proced for immunohisto, please suggest me some fixative also thanks in advance Arvind Singh Pundir, Deptt. of Neuroanatomy, National Brain Research centre , haryana , INDIA From Hedley.Glencross <@t> CMMC.nhs.uk Wed Mar 9 02:03:04 2005 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:24:44 2005 Subject: [Histonet] Immunocytochemistry on cytospins Message-ID: Hi Gudrun ICC on cytospins is easier if you follow a few fundamentals. Fresh samples are best, and a monolayer must be achieved. This requires experimentation, but with experience the dilution factors and spinning times can be found. Air dried samples are OK, with post fixation in cold acetone, but if you decide to fix then as suggested light fixation is all that is needed, although I have kept samples fixed up to 4 days without too much problem. However, for suspected lymphomas air dried cytospins are best. Lastly, if you do a peroxidase method this will need a long block (around 30 minutes) in both hydrogen peroxide and non immune serum to reduce background. Have fun Good luck Hedley Glencross Manchester Cytology Centre Message: 1 Date: Tue, 8 Mar 2005 10:36:01 -0600 From: "GUTIERREZ, JUAN" Subject: RE: [Histonet] cellsmears and cytospin for ihc To: , "Histonetliste (Histonetliste)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We do a few markers regularly on cytospins and or smears. The general rule is to fix in 95% alcohol right after spinning, do not let them air-dry. From the alcohol we then transfer to the immuno buffer and go from there. We have had good results with storing the cytospins in the same alcohol for short periods of time, but I wouldn't try it for very long. Good luck. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, March 04, 2005 10:54 AM To: Histonetliste (Histonetliste) Subject: [Histonet] cellsmears and cytospin for ihc Hi histonetters, We perform ihc on cellsmears and cytospins very rarely. Therefore we have no really experience. Can anyone tell me, if there is a general preperation protocol for these specimen, that is best for ihc? Air-drying / fixation in . / storage ? Or does it depend on the antibody or the kind of fluid? Thanks in advance Gudrun Lang Linz, Austria From mikael.niku <@t> helsinki.fi Wed Mar 9 05:28:51 2005 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC humidity chamber? In-Reply-To: Message-ID: <000d01c5249b$2b323a50$8c0fd680@ekk1116> Hi! If you're doing lots of slides, you might try a big cake box by Tupperware. The box is about 50cm x 30 cm x 15 cm. It includes a tray with has sort of rails or bars which make excellent stands for the slides. We put wet paper towels on the bottom, then stack several trays in the box, and this way we can fit in a massive number of slides. Really the only drawback was that it was originally transparent, so I needed to paint/tape it. I'm not sure if these boxes are still available, though. Anyway, we later found out it's much better to use Shandon Coverplates for immunohistochemistry and in situ hybridization. We are nowadays doing almost everything in them. Just the racks and the plates, that's all you need. The racks are terribly expensive but worth it if you're doing lots of immunos. Samples can never dry out, and you save in reagent volumes, processing time, and your nerves. //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of J m > Sent: 7. maaliskuuta 2005 18:27 > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC humidity chamber? > > > salut, > > Does anyone know of a supplier in Canada for the IHC humidity > chambers. I > used to have a black plastic one with a clear lid but I don't > know where it > came from or where i can get another one. I am tired of > having slides dry > out during overnight incubation in the make-shift chambers I > have tried to > construct. Regular tissue sections seem to "hold" the > solution on the slide > better than tissue arrays which are more apt to losing there > solutions. I > don't like using water repelent pens to circle the arrays but any > suggestions would be appreciated. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Andrew.Prior <@t> Smith-Nephew.com Wed Mar 9 06:55:35 2005 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Re: replies to Histonet Digest Message-ID: To All Histonetters. Just to add to what Gayle said. Please don't reply to Histonet digest - for those of us who receive the digest it becomes impossible to find where one digest ends and the latest one restarts. Just cut and paste the relevant message and add a new subject line. This will help you get a response for 2 reasons: 1- people won't automatically delete your message as Gayle said she tended to do (as do I) when they see RE:Histonet Digest as the subject line. And 2 - you are less likely to be thought of as a fool (no offence) who can't follow simple instructions and guidelines for posting to a list server. (this will be where someone reminds me of one of my mistakes when posting....) Most people will happily try to help others on Histonet, even if questions are very basic, but get fed up when they have to hunt for ages to work out what the message is. I enjoy reading the postings on the digest, even those that aren't directly relevant to my field, and have learnt a great deal. Digest replies drive me nuts though. Histonet is a wonderful resource so please use it wisely. Andrew Andrew Prior Histologist Smith & Nephew Research Centre York Science Park Heslington York >>Message: 6 Date: Tue, 08 Mar 2005 12:45:05 -0700 From: Gayle Callis Subject: [Histonet] People who receive and send replies to Histonet Digest so we see RE: Histonet Digest To: hymclab , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050308123537.01c20e40@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Dear all who receive Histonet Digest, Don't send a reply to Histonet Digest - please. It kicks back all the messages to those who do not use the digest format, something they may not want to get again and again. Some of us tend to delete RE: Histonet Digest since there is nothing specific in the subject line. Send your answers to: Histonet@lists.utsouthwestern.edu. AND use the subject line with specific information to alert others you have commentary, answers to questions, or inquiries. This way all those who do not using digest format are not overloaded with uyneeded unwanted ton of daily messages. Thanks, folks Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. From Stephen.J.Scholz <@t> osfhealthcare.org Wed Mar 9 07:33:07 2005 From: Stephen.J.Scholz <@t> osfhealthcare.org (Scholz, Stephen J.) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Cassette printers Message-ID: <7F1312711CA7474A89B3DF8BA0BA54D0F5F1A2@pmc-rfd-mx01.intranet.osfnet.org> Hello all; Is there anyone out there who has set up a cassette and slide printer to their Copath information system. If you have I have several questions. Is it easy to use? Can you modify the data and print a just a couple of blocks after the initial data input and not have to print the whole case? What type of software support did you need for the printers to talk to Copath and about how much did it cost. I'm in a pickle with these printers and I don't know what direction to go. Any help would be greatly appreciated. Please feel free to call. Stephen J. Scholz HT(ASCP) Histology Coordinator OSF St. Anthony Medical Center Rockford IL Phone: 815-395-5410 Fax: 815-395-5364 From sladd <@t> hsc.usf.edu Wed Mar 9 08:32:46 2005 From: sladd <@t> hsc.usf.edu (Ladd, Sharron) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] 2003 ASCP/BOR Wage and Vacancy Survey HTL or HLT? Message-ID: <841D767DCDE87C49A02DA6DFC0BABABF674693@COMEXCHANGE.hscnet.hsc.usf.edu> I just received the March 2005 issue of LabMedicine which contains the 2003 ASCP/BOR Wage and Vacancy Survey. Unfortunately, the authors and editors were unable to remember from page to page whether the abbreviation for Histotechnologist was HTL or HLT? They got it right in the methods section, got it wrong in the big table (Table 2) and then wrote it both ways in the vacancy section of the Histotechnician/Histotechnologist portion of the article. Sharron Ladd, HTL or HLT (ASCP)?? Hmmmmm USF From AFeatherstone <@t> KaleidaHealth.Org Wed Mar 9 09:24:15 2005 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] The Use of Placenta in the Understanding of Perinatal Injury Message-ID: Am looking for information on collection, fixation and storage of placentas. Is anyone out there doing pathology on all placentas from there institution. If so, what are you doing for storage. Do you freeze any tissue for possible in-situ studies? thanks in advance Annette -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Tuesday, March 08, 2005 10:04 To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] PA -----Original Message----- From: Charles.Embrey Sent: Tuesday, March 08, 2005 9:04 AM To: 'lpwenk@sbcglobal.net' Subject: RE: [Histonet] PA Peggy, The $450 fee is what the ASCP is charging the current AAPA fellows for certification. We have been told that that will be the exam fee. If the actual exam fee is lower then I know several hundred AAPA fellows that pay $450 this year will be highly upset. The new certificates have been received by several of my fellow PA's and are very nicely done. You can see the fee listed on the ASCP application at: ftp://ftp.ascp.org/Grab/PDFs/BORpdf/APPLICATIONPST.pdf Any new news I get I will pass on to the Histonet as well. Charles Embrey Pathologists' Assistant -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lpwenk@sbcglobal.net Sent: Tuesday, March 08, 2005 4:33 AM To: Histonet Subject: [Histonet] PA Questions arose about the exam content and cost for the ASCP PA certification. There is a committee working on this (with members from ASCP, AAPA, other organizations). They expect to publish in the Spring of 2005. I'll keep an eye on the ASCP BOR webpage, and let the histonet community know when this information is available. As for the cost of the PA exam - that has not been established either. But from the cost of the other tests: http://www.ascp.org/bor/application/fees.asp - phlebotomists - $90 - technicians - $125 - technologists - $145 - specialists - $185 - diplomate (manager/supervisor) - $250 Notice, it relates to the salary - those who make more money, pay a higher application fee. I don't know where the $450 figure, mentioned in one email, is coming from. There is nothing on the ASCP BOR webpage about the PA application fee. And that mentioned figure just seems way out of line from the other fees already established. So let's just wait until the fee is published, OK? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From MSafron <@t> wilresearch.com Wed Mar 9 09:21:20 2005 From: MSafron <@t> wilresearch.com (Mike Safron) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Paraplast Plus Message-ID: We are using Paraplast Plus from Kendall (Tyco/Healthcare). We have noticed that after the paraffin melts (in embedding center and paraffin pot) that there is what appears to be a straw colored liquid that is deposited at the bottom of the units. It would appear that the liquid is not miscible with the melted paraffin. This liquid is causing problems with embedding. Does anybody know where this liquid is coming from? Michael Safron A.S.,HT(ASCP) Manager, Histology WIL Research Laboratories LLC 419-289-8700 ext: 3040 Ashland, Ohio 44805 From jkiernan <@t> uwo.ca Wed Mar 9 09:40:27 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] The subject line in Histonet emails. Message-ID: <422F18EB.A64D5FD4@uwo.ca> Well said, Gayle! The subject line is the MOST IMPORTANT PART of any email. (No apology for shouting this in capital letters) If you're asking a question it's worth thinking hard about those 7 or 8 words that indicate what the question is about. Recent good Histonet examples are: oxalate crystals removal of carbohydrates/glycosylation For answering a question, it's fine to "reply to all." The subject line will then be: Re: removal of carbohydrates/glycosylation (Did anyone answer this? A fascinating question, and I'd also like to know the answer. Biochemists must have been doing this for decades.) [This comment is an example of something that's not covered by the subject line, so I don't expect an answer. It's an example of what not to do.] Often, with replies to replies to replies, the true subject becomes completely different from that of the original email. If you write something that changes the subject, change the subject line too, so that we'll all know what the message is about. A useful trick has the form: Polar micro also for amyloid (Was Re: oxalate ...) This might help someone else looking at renal pathology, without entirely losing track of the thread of how to examine for crystals. Our beloved listserver now adds [Histonet] to the front end of every subject line. This increases the need for brevity. Gayle's message that prompted this one is quoted below. It showed up in the summary list as [Histonet] People who receive ... A longer line (but garbled and uninformative) appeared when my mouse cursor paused over the item. Brief subject lines are a must. End of rant. John Kiernan London, Canada ___________________________ Gayle Callis wrote: > Subject: [Histonet] People who receive and send replies to > Histonet Digest so we see RE: Histonet Digest > > Dear all who receive Histonet Digest, > > Don't send a reply to Histonet Digest - please. It kicks back all the > messages to those who do not use the digest format, something they may not > want to get again and again. Some of us tend to delete RE: Histonet Digest > since there is nothing specific in the subject line. > > Send your answers to: Histonet@lists.utsouthwestern.edu. AND use the > subject line with specific information to alert others you have commentary, > answers to questions, or inquiries. > This way all those who do not using digest format are not overloaded with > unneeded unwanted ton of daily messages. > > Thanks, folks > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > ---------------------------------------------- From cmichelini <@t> esbe.com Wed Mar 9 09:51:00 2005 From: cmichelini <@t> esbe.com (Carla Michelini) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] humidity chambers Message-ID: In Canada, ESBE Scientific sells the Thermobrite unit which is ideal for IHC as a humidity chamber. You may contact our main phone number at 1-800-268-3477 or your local ESBE Scientific representative. Carla Michelini, MLT, CCPE Product Manager ESBE Scientific 80 McPherson St Markham, Ontario L3R 3V6 1-800-268-3477 ext 309 www.esbe.com From tramjiganesh <@t> rics.bwh.harvard.edu Wed Mar 9 10:03:29 2005 From: tramjiganesh <@t> rics.bwh.harvard.edu (Tripu Ramjiganesh) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] (no subject) Message-ID: Hi, I have fixed some E13.5 and E16.5 mouse hearts in 4% PFA O/N and now i am thinking of doing confocal microscopy. What is the best way to embed? I see in the literature that cryo sections are better, since i already fixed them what would be the best bet? Thanks RT From akbitting <@t> geisinger.edu Wed Mar 9 10:03:16 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Paraplast Plus Message-ID: I've heard (from a Surgipath rep) that they have has problems with water in the past. Just thought I'd pass this on. Don't know how true it is. >>> "Mike Safron" 03/09/05 10:21 AM >>> We are using Paraplast Plus from Kendall (Tyco/Healthcare). We have noticed that after the paraffin melts (in embedding center and paraffin pot) that there is what appears to be a straw colored liquid that is deposited at the bottom of the units. It would appear that the liquid is not miscible with the melted paraffin. This liquid is causing problems with embedding. Does anybody know where this liquid is coming from? Michael Safron A.S.,HT(ASCP) Manager, Histology WIL Research Laboratories LLC 419-289-8700 ext: 3040 Ashland, Ohio 44805 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Linke_Noelle <@t> Allergan.com Wed Mar 9 10:04:01 2005 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] mouse macrophages Message-ID: Hi everyone! Does anyone have a good quality antibody/protocol to detect macrophages in FFPE mouse tissues? Thank you!! Noelle Linke, BS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From pruegg <@t> ihctech.net Wed Mar 9 10:22:01 2005 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] EGFR in FFPE mouse kidney In-Reply-To: <20050309023315.76330.qmail@web90002.mail.scd.yahoo.com> References: <20050309023315.76330.qmail@web90002.mail.scd.yahoo.com> Message-ID: <4466.67.38.6.185.1110385321.squirrel@67.38.6.185> Brade, I would recommend trying Lab Vision's rabbit monoclonal EGFR on your mouse kidneys. Patsy > Hi Histonetters, > I have been really struggling trying to get EGFR staining in the tubles of > mouse kidneys. I hope that somewhere out there has had good success > staining EGFR in mouse kidneys. I know that there are a lot of antibodies > out there.....but I have a feeling some or alot of you out there already > know the answere. Thank you soo much for your help.......brade > > > --------------------------------- > Celebrate Yahoo!'s 10th Birthday! > Yahoo! Netrospective: 100 Moments of the Web > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Wed Mar 9 10:26:15 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Inexpensive humidity chambers for IHC Message-ID: <6.0.0.22.1.20050309085713.01b58590@gemini.msu.montana.edu> Although you live in Canada, you could access Evergreen Scientific for there tidy little Immuno Stain Moisture Chamber either in clear or black, hold 10 slides per box with each slide in individual slots. Approx $80/pkg of 3 boxes. http://www.evergreensci.com or I have seen these boxes from other vendors but do not recall where. These boxes very efficient, cheap and fit in incubators, desktops or refrigerators. We use them frequently for cold temperature antibody incubation. Evergreen also manufactures for other companies, and they may have a source in Canada. Also Scytek (Logan Utah) has humidity chamber. Holds 20 slides with drip tray, slide holder tilts easy rinsing, slides stay in place from start to finish, comes with white plastic inserts to put in back of slides to observe chromogen development, and clear plastic lids over slide holders for easy access to slides for reagent application. SlideMasters are approx $400 US dollars. They fit in a refrigerator, incubator, or on bench top. I have 6 of them, and love using them. Go to www.scytek.com website. We often set up 5 Slidemasters for one days staining of 100 slides. Great for a small laboratory looking for more efficient manual IHC work. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From tramjiganesh <@t> rics.bwh.harvard.edu Wed Mar 9 10:39:38 2005 From: tramjiganesh <@t> rics.bwh.harvard.edu (Tripu Ramjiganesh) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Confocal microscopy Message-ID: Hi, I have fixed some E13.5 and E16.5 mouse hearts in 4% PFA O/N and now i am thinking of doing confocal microscopy. What is the best way to embed? I see in the literature that cryo sections are better, since i already fixed them what would be the best bet? Thanks for your help RT From pruegg <@t> ihctech.net Wed Mar 9 10:35:50 2005 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet]confocal microscopy In-Reply-To: References: Message-ID: <4502.67.38.6.185.1110386150.squirrel@67.38.6.185> RT, The reason frozen sectioning is recommended for CF is that flurescent labels are often used and usually work best on frozens, you can rinse the PFA from your fixed hearts with PBS or Tris buffer and then try freezing them to make sections for IHC. Patsy > Hi, I have fixed some E13.5 and E16.5 mouse hearts in 4% PFA O/N and > now i am thinking of doing confocal microscopy. What is the best way to > embed? I see in the literature that cryo sections are better, since i > already fixed them what would be the best bet? > Thanks > RT > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Wed Mar 9 10:40:42 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Paraplast Plus In-Reply-To: References: Message-ID: <6.0.0.22.1.20050309093058.01b762e0@gemini.msu.montana.edu> We had trouble with this paraffin in the past: Several things: Although the droplet appear not miscible, have you tried stirring the paraffin after melting to redistribute the plastic polymer additives, etc that are in the paraffin. This should be done before embedding, and before adding any melted paraffin to processor paraffin containers, pots. During processing, agitaion should keep these resuspended. Stirring paraffin before embedding EACH DAY is advisable to keep additives (heavier so they settle to bottom of paraffin pots) suspended or you will run into sectioning problems. A plastic spatula is very handy for this purpose. If stock paraffin is stored in a cold place and then melted, water condenses and water droplets form in bottom of paraffin pots/storage containers. Store your stock paraffin at recommended tempertures, cool, but not extremely cold place, dry. We found room temperature storage was adequate prior to melting. Also, do not melt Paraplast Plus over 62C, high temperatures create havoc with this paraffin, and you may damage additives - we never exceeded 62C for melting, storage or processing protocols. Poor sectioning occured with high temperature melting of this paraffin and also not stirring it before embedding or dispensing into paraffin processor pots. Clean storage pots and embedding centers frequently. and At 08:21 AM 3/9/2005, you wrote: >We are using Paraplast Plus from Kendall (Tyco/Healthcare). We have noticed >that after the paraffin melts (in embedding center and paraffin pot) that >there is what appears to be a straw colored liquid that is deposited at the >bottom of the units. It would appear that the liquid is not miscible with >the melted paraffin. This liquid is causing problems with embedding. Does >anybody know where this liquid is coming from? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From ian.montgomery <@t> bio.gla.ac.uk Wed Mar 9 10:47:59 2005 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:24:45 2005 Subject: Fwd: RE: [Histonet] UK prices[Scanned] Message-ID: <6.2.1.2.2.20050309164316.0300bdf0@udcf.gla.ac.uk> Just been doing this type of thing myself for a full economic costing exercise. One piece of tissue, tray of slides stained with H&E = ?60. You would be better giving me several pieces of tissue as the price drops, considerably, per specimen. Ian. >From: Kemlo Rogerson >To: "'Edwards, R.E.'" , > histonet@lists.utsouthwestern.edu >Date: Fri, 4 Mar 2005 07:49:03 -0000 >X-Mailer: Internet Mail Service (5.5.2653.19) >X-OriginalArrivalTime: 04 Mar 2005 07:48:49.0684 (UTC) > FILETIME=[9A582540:01C5208E] >X-Scan-Signature: 02458d915c013d2bafb5a1ca86d08841 >Cc: >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: fafe997e7c8aede70e4d53d18f8e9e53 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: RE: [Histonet] UK prices[Scanned] >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >X-GLA-Spam-Scan: R >X-GLA-Spam-Score: 0.0 (/) >X-GLA-Spam-Report: > >Some years ago it was ?5 for processing, cutting and staining an H&E. > >-----Original Message----- >From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] >Sent: 03 March 2005 15:49 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] UK prices[Scanned] > > > > I need to get some idea of how much it costs to process, cut and >stain a block of tissue, any help/ideas most gratefully received. > Richard Edwards > MRC TOXICOLOGY UNIT > LEICESTER...U.K....... > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07623 975451 e-mail: ian.montgomery@bio.gla.ac.uk From RitaR <@t> lexhealth.org Wed Mar 9 10:58:58 2005 From: RitaR <@t> lexhealth.org (Rita Riddle) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Cobalt Chloride in Processor Message-ID: Hello Everyone, I need some help. Can you tell me about Cobalt Chloride in the processor to stain the tissue. At what station do you add this to, how much do you use. Also would like to know if the blue staining affect the H&E in anyway. What about the embedding also. Any information regarding this will be most helpful. Thanks so much, Rita Riddle Lead Histology Tech Lexington Medical Center 2720 Sunset Blvd. West Columbia, SC 29169 803-791-2881 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From bwhitaker <@t> brownpathology.com Wed Mar 9 11:11:08 2005 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Houston Job Opportunity Message-ID: <000001c524ca$fc5d8bf0$3601a8c0@brownpathology.net> Hi All! I currently have a histo tech position open. The hours are 4:00am-12:30pm. The group is a private practice pathology group that has been around for over 50 years, but the laboratory is less than a year old. The benefits are great, including free, on-site parking. Our location is near the medical center at I-610 S at Almeda Rd. If you are interested, please fax or email your resume, or give me a call. Thanks, Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 Phone 713-741-6677 Fax 713-748-5860 bwhitaker@brownpathology.com From mcauliff <@t> umdnj.edu Wed Mar 9 13:57:17 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] mouse macrophages In-Reply-To: References: Message-ID: <422F551D.20406@umdnj.edu> You might get results with F4/80, although you may need antigen retrieval. Geoff Linke_Noelle wrote: >Hi everyone! > > > >Does anyone have a good quality antibody/protocol to detect macrophages >in FFPE mouse tissues? > > > > > >Thank you!! > > > > > > > >Noelle Linke, BS, HTL(ASCP)QIHC > >Allergan, Inc > >2525 Dupont Drive RD-2A > >Irvine, CA 92612 > >714-246-5568 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From SnyderW <@t> uhcwv.org Wed Mar 9 12:02:56 2005 From: SnyderW <@t> uhcwv.org (Snyder, Wendy) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Paraplast problems Message-ID: <7B5CC54F6E30BD42A8C61E4896484D19011A9217@uhc-exchange.uhc.wvuhs.com> Histonetters, We have also been experiancing problems with the Paraplast extra. We get a lot of air bubbles in the embedded mold. Has anyone else had this problem? Wendy Snyder HT(ASCP) United Hospital Center Clarksburg, WV From julien_lambreydesouza <@t> uqar.qc.ca Wed Mar 9 12:32:26 2005 From: julien_lambreydesouza <@t> uqar.qc.ca (Julien LambreyDeSouza) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] problem sending pics to the histonet Message-ID: <6.1.1.1.1.20050309133107.0199eb50@pop3.uqar.qc.ca> How do you send picture files (jpg) to the histonet? On histonet.org it is mentioned to send pics to pictures@histonet.org, but this is not working. Thanks, Julien Lambrey de Souza Biologie ?volutive, Universit? du Qu?bec ? Rimouski (418) 723-1986 #1714 From mucram11 <@t> comcast.net Wed Mar 9 12:56:43 2005 From: mucram11 <@t> comcast.net (pam marcum) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Paraplast Plus References: <6.0.0.22.1.20050309093058.01b762e0@gemini.msu.montana.edu> Message-ID: <422F46EB.000005.04084@YOUR-4DI1S53IME> Gayle is giving the best advise you can get for any paraffin on the market today. They all have additives and these problems can occur all of them if not properly stirred and mixed daily. One question I have is do you allow the paraffin to re-solidify overnight? IF so this can add to the problem. Pam Marcum -------Original Message------- From: Gayle Callis Date: 03/09/05 11:41:55 To: Mike Safron; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Plus We had trouble with this paraffin in the past: Several things: Although the droplet appear not miscible, have you tried stirring the paraffin after melting to redistribute the plastic polymer additives, etc that are in the paraffin. This should be done before embedding, and before adding any melted paraffin to processor paraffin containers, pots. During processing, agitaion should keep these resuspended. Stirring paraffin before embedding EACH DAY is advisable to keep additives (heavier so they settle to bottom of paraffin pots) suspended or you will run into sectioning problems. A plastic spatula is very handy for this purpose. If stock paraffin is stored in a cold place and then melted, water condenses and water droplets form in bottom of paraffin pots/storage containers. Store your stock paraffin at recommended tempertures, cool, but not extremely cold place, dry. We found room temperature storage was adequate prior to melting. Also, do not melt Paraplast Plus over 62C, high temperatures create havoc with this paraffin, and you may damage additives - we never exceeded 62C for melting, storage or processing protocols. Poor sectioning occured with high temperature melting of this paraffin and also not stirring it before embedding or dispensing into paraffin processor pots. Clean storage pots and embedding centers frequently. and At 08:21 AM 3/9/2005, you wrote: >We are using Paraplast Plus from Kendall (Tyco/Healthcare). We have noticed >that after the paraffin melts (in embedding center and paraffin pot) that >there is what appears to be a straw colored liquid that is deposited at the >bottom of the units. It would appear that the liquid is not miscible with >the melted paraffin. This liquid is causing problems with embedding. Does >anybody know where this liquid is coming from? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amoklebu <@t> seattlecca.org Wed Mar 9 12:56:58 2005 From: amoklebu <@t> seattlecca.org (Moklebust, Amanda C) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] LCA antibody for B5 tissue Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF3D9@wala01.seattlecca.org> Dear Histonetters, Do any of you know of a clone for CD45-LCA that stains B5 fixed tissue? We are using a clone from DakoCytomation, anti-CD45, T29/33. It stains formalin fixed tissue, but I see no staining in the B5 fixed tonsil control. I have tried different blocks of tonsil with the same result. All of the blocks stain with CD3 and CD20, so my guess is that this antibody does not recognize the antigen after B-5 fixation. Please let me know if you have had any success with CD45-LCA in B5 fixed tissue. Replies telling me not to use B5 are not helpful. I know it has a lot of negative qualities, and I would love to avoid the whole issue. Unfortunately, I have to work with an archive of tissue fixed in B5. Thanks for your assistance, Amanda Moklebust, HTL(QIHC) Seattle Cancer Care Alliance This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From Barry.R.Rittman <@t> uth.tmc.edu Wed Mar 9 13:08:03 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Paraplast Plus Message-ID: <566FB0B522443D43AF02D2ADBE35A6F001492865@UTHEVS3.mail.uthouston.edu> One point that has not yet been mentioned here is that paraffin is not a single compound but a mixture of compounds with various compositions and melting points. This is evident if you heat paraffin for some hours you can drive off the lower melting point components etc. You can also change melting point and cutting properties by the addition of various substances such as ceresin. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam marcum Sent: Wednesday, March 09, 2005 12:57 PM To: MSafron@wilresearch.com; Histonet@lists.utsouthwestern.edu; gcallis@montana.edu Subject: Re: [Histonet] Paraplast Plus Gayle is giving the best advise you can get for any paraffin on the market today. They all have additives and these problems can occur all of them if not properly stirred and mixed daily. One question I have is do you allow the paraffin to re-solidify overnight? IF so this can add to the problem. Pam Marcum -------Original Message------- From: Gayle Callis Date: 03/09/05 11:41:55 To: Mike Safron; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraplast Plus We had trouble with this paraffin in the past: Several things: Although the droplet appear not miscible, have you tried stirring the paraffin after melting to redistribute the plastic polymer additives, etc that are in the paraffin. This should be done before embedding, and before adding any melted paraffin to processor paraffin containers, pots. During processing, agitaion should keep these resuspended. Stirring paraffin before embedding EACH DAY is advisable to keep additives (heavier so they settle to bottom of paraffin pots) suspended or you will run into sectioning problems. A plastic spatula is very handy for this purpose. If stock paraffin is stored in a cold place and then melted, water condenses and water droplets form in bottom of paraffin pots/storage containers. Store your stock paraffin at recommended tempertures, cool, but not extremely cold place, dry. We found room temperature storage was adequate prior to melting. Also, do not melt Paraplast Plus over 62C, high temperatures create havoc with this paraffin, and you may damage additives - we never exceeded 62C for melting, storage or processing protocols. Poor sectioning occured with high temperature melting of this paraffin and also not stirring it before embedding or dispensing into paraffin processor pots. Clean storage pots and embedding centers frequently. and At 08:21 AM 3/9/2005, you wrote: >We are using Paraplast Plus from Kendall (Tyco/Healthcare). We have noticed >that after the paraffin melts (in embedding center and paraffin pot) that >there is what appears to be a straw colored liquid that is deposited at the >bottom of the units. It would appear that the liquid is not miscible with >the melted paraffin. This liquid is causing problems with embedding. Does >anybody know where this liquid is coming from? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Mar 9 13:48:47 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:45 2005 Subject: water contamination in Re: [Histonet] Paraplast Plus In-Reply-To: References: Message-ID: <6.0.0.22.1.20050309124650.01b897d0@gemini.msu.montana.edu> This is what we experienced in the past, but one could take a few droplets off bottom of paraffin pot with a plastic pasteur pipette and touch it to a dark paper towel to see if it is wetted versus the feel of paraffin on towel. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Wed Mar 9 13:57:59 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Two potential problems here Re:confocal microscopy In-Reply-To: <4502.67.38.6.185.1110386150.squirrel@67.38.6.185> References: <4502.67.38.6.185.1110386150.squirrel@67.38.6.185> Message-ID: <6.0.0.22.1.20050309124938.01b971d0@gemini.msu.montana.edu> First, if you fix with PFA, you need to cryoprotect the prefixed tissues with 25 to 30% sucrose overnight or longer (tissue should sink to bottom of solution) or you will have poor frozen sections. Just rinsing with PBS is still keeping high water concentration in the tissues for water ice formation problems. Secondly, if you snap freeze OCT embedded embryos without fixation, in other words fresh embryos, you will eliminate increased autofluorescence problems created by paraformaldehyde fixation. If they are already fixed, then you can still cryoprotect, and do snap freezing, cryosectioning or you can try paraffin embedding. Autofluorescence may be present at a level you can work with or deal with autofluorescence by using a fluorophore that is a contrasting color i.e. red rather than yellowish-green. Alexa 555 from Molecular Probes can be found conjugated to secondary antibodies and Strepavidin. Alexa fluorophores are more stable, less photobleaching when using Confocal or CLSM microscopes. At 09:35 AM 3/9/2005, you wrote: >RT, >The reason frozen sectioning is recommended for CF is that flurescent >labels are often used and usually work best on frozens, you can rinse the >PFA from your fixed hearts with PBS or Tris buffer and then try freezing >them to make sections for IHC. >Patsy > > Hi, I have fixed some E13.5 and E16.5 mouse hearts in 4% PFA O/N and > > now i am thinking of doing confocal microscopy. What is the best way to > > embed? I see in the literature that cryo sections are better, since i > > already fixed them what would be the best bet? > > Thanks > > RT > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From ploykasek <@t> phenopath.com Wed Mar 9 14:29:03 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] LCA antibody for B5 tissue In-Reply-To: <5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF3D9@wala01.seattlecca.org> Message-ID: Amanda, I?m almost positive I?ve had this antibody work on B5 fixed tissues, mainly bone marrows. What type of antigen retrieval are you using? This could make a difference. Are you doing any type of treatment to remove the precipitated mercuric chloride? This can lead to false negatives with some antibodies. I?ve routinely used a citrate based heat pretreatment. This recovers the antigen nicely and removes the mercuric chloride(in most cases). Hope this helps. Patti Loykasek PhenoPath Laboratories Seattle, WA> > > Dear Histonetters, > > Do any of you know of a clone for CD45-LCA that stains B5 fixed > tissue? We are using a clone from DakoCytomation, anti-CD45, T29/33. It > stains formalin fixed tissue, but I see no staining in the B5 fixed tonsil > control. I have tried different blocks of tonsil with the same result. All > of the blocks stain with CD3 and CD20, so my guess is that this antibody > does not recognize the antigen after B-5 fixation. > > Please let me know if you have had any success with CD45-LCA in B5 > fixed tissue. Replies telling me not to use B5 are not helpful. I know it > has a lot of negative qualities, and I would love to avoid the whole issue. > Unfortunately, I have to work with an archive of tissue fixed in B5. > > Thanks for your assistance, > > Amanda Moklebust, HTL(QIHC) > Seattle Cancer Care Alliance > > > > This electronic message transmission contains information which may be > confidential or privileged. The information is intended to be for the > use of the individual or entity named above. If you are not the > intended recipient, be aware that any disclosure, copying, distribution > or use of the contents of this information is prohibited. If you have > received this electronic transmission in error, please leave a message > via telephone at (206) 288-6266, notify me by electronic reply, and > delete this message. Opinions and ideas in this message that do not > relate to official business are understood as neither given nor > endorsed by the Seattle Cancer Care Alliance. To view our complete > Notice of Privacy Practices, visit our web site at www.seattlecca.org. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amoklebu <@t> seattlecca.org Wed Mar 9 15:11:55 2005 From: amoklebu <@t> seattlecca.org (Moklebust, Amanda C) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] LCA antibody for B5 tissue Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF3DE@wala01.seattlecca.org> Patti, I have been pretreating the slides with Dako's Target Retrival Solution pH 6.1 in a steamer for 20 minutes. The slides do not get treated to remove mercury pigment. I've gotten two replies recommending a different clone (2B11/PD7/26) from Dako. I don't think they were posted to everyone. Thanks for the help. Amanda -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Wednesday, March 09, 2005 12:29 PM To: histonet Subject: Re: [Histonet] LCA antibody for B5 tissue Amanda, I?m almost positive I?ve had this antibody work on B5 fixed tissues, mainly bone marrows. What type of antigen retrieval are you using? This could make a difference. Are you doing any type of treatment to remove the precipitated mercuric chloride? This can lead to false negatives with some antibodies. I?ve routinely used a citrate based heat pretreatment. This recovers the antigen nicely and removes the mercuric chloride(in most cases). Hope this helps. Patti Loykasek PhenoPath Laboratories Seattle, WA> This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From emerald_lake77 <@t> yahoo.com Wed Mar 9 15:17:42 2005 From: emerald_lake77 <@t> yahoo.com (- -) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Commonly used stains for kidney disease progession?? Message-ID: <20050309211742.56409.qmail@web42301.mail.yahoo.com> Hi everyone, Quick question: What are the commonly used stains/immunochemistry procedures to evaluate progression of disease within kidneys? (I may need to know WHY also in case I am not familiar with a certain technique, stain, or antibody) So far I have been told a number ranging from PAS, trichrome and a silver technique - but I started to become confused. They all seem great but I think I may need the WHY along with the WHAT when answers are being sent back in reply. As usual, thank you for your assistance. G.T. Hebert Cambridge Massachusetts --------------------------------- Celebrate Yahoo!'s 10th Birthday! Yahoo! Netrospective: 100 Moments of the Web From ohenry <@t> dfw.net Wed Mar 9 16:28:31 2005 From: ohenry <@t> dfw.net (Susan Owens) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] 'CAP' regs web site/link Message-ID: <001801c524f7$54906b40$53dd3040@Nationwide.net> Can anyone direct me to a web site where I can see the currant CAP regs???? Main point of interest is grossroom/cyrostat disinfection/cleaning. I did have some sites/links but they don't seem to be working. Susan Owens-TX ohenry@dfw.net From Jackie.O'Connor <@t> abbott.com Wed Mar 9 16:32:48 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] It's a mystery . . . Message-ID: Hi Histonetskis - Maybe I'm thinking too hard - I can't seem to get rid of false positive staining in negative control sections of human and mouse kidney human and pancreas tumor. I'm using EDTA HIER (for the first time), in an SABC protocol with a Goat Pab. I've tried two different (vendor) Rab anti Goat biotinylated secondarys. I'm using an avidin/biotin block - each for 20 minutes, (two different lots/vendors) but I'm still getting this false staining in the negative (omitted primary) control sections. Is it possible to not be able to block ALL biotin? I've never seen this before. I can't imagine what else I'm seeing - but like I said - my brain is tired. Before I switch to an HRP conjugated secondary - can anyone suggest what else this could be? I know EDTA can cause background, but there is no primary on these sections. Will someone loan me their brain? I can't help but wonder if the EDTA is causing my mystery - but I have to use it. Help me, Obi-wan-kenobi. Jackie O' From bwhitaker <@t> brownpathology.com Wed Mar 9 17:00:05 2005 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Polyoma virus probe Message-ID: <000701c524fb$bc7f55a0$3601a8c0@brownpathology.net> Hi All, A friend asked me about a good probe for polyoma virus. Does anyone have any recommendations? Thanks, Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 From bhewlett <@t> cogeco.ca Wed Mar 9 17:23:36 2005 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] LCA antibody for B5 tissue References: <5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF3DE@wala01.seattlecca.org> Message-ID: <004601c524ff$05150050$6500a8c0@mainbox> Amanda, We have used Dako's M0701, clones (2B11 + PD7/26) on B5 fixed material for lo' these many years with NO trouble. It's even mentioned on the product data sheet. HIER is not required! However, you should treat B5-fixed material with the iodine/hypo sequence to remove mercury fixation pigments(even if you don't see them). Dilution=1:100 incubate 60 min @ RT. Bryan ----- Original Message ----- From: "Moklebust, Amanda C" To: "'Patti Loykasek'" ; "histonet" Sent: Wednesday, March 09, 2005 4:11 PM Subject: RE: [Histonet] LCA antibody for B5 tissue Patti, I have been pretreating the slides with Dako's Target Retrival Solution pH 6.1 in a steamer for 20 minutes. The slides do not get treated to remove mercury pigment. I've gotten two replies recommending a different clone (2B11/PD7/26) from Dako. I don't think they were posted to everyone. Thanks for the help. Amanda -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Wednesday, March 09, 2005 12:29 PM To: histonet Subject: Re: [Histonet] LCA antibody for B5 tissue Amanda, I?m almost positive I?ve had this antibody work on B5 fixed tissues, mainly bone marrows. What type of antigen retrieval are you using? This could make a difference. Are you doing any type of treatment to remove the precipitated mercuric chloride? This can lead to false negatives with some antibodies. I?ve routinely used a citrate based heat pretreatment. This recovers the antigen nicely and removes the mercuric chloride(in most cases). Hope this helps. Patti Loykasek PhenoPath Laboratories Seattle, WA> This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.gorrie <@t> unsw.edu.au Wed Mar 9 17:27:11 2005 From: c.gorrie <@t> unsw.edu.au (Cath Gorrie) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] connector hose? Message-ID: Hi, Wondering of anybody can help me out before I go buying a new one of these. I'm looking for a connector hose from a nitrous oxide gas cylinder to a halothan set-up. It's a blue hose with fittings on either end to attach to regulator at the gas cylinder and a similar fitting on the other end. Maybe someone would have one of these laying around that they don't use anymore. It may be quite generic in some labs, certainly not in ours. If you have a spare and are willing to part with it, I'd really appreciate it. Regards, Cathy ---------------------------------------------------------------------------- Catherine Gorrie School of Medical Sciences University of New South Wales Sydney, NSW ph: 02 9385 2462 fax: 02 9385 8016 e-mail: c.gorrie@unsw.edu.au ---------------------------------------------------------------------------- From marktarango <@t> earthlink.net Wed Mar 9 17:35:40 2005 From: marktarango <@t> earthlink.net (Mark Adam Tarango) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Re: Histonet Digest, Vol 16, Issue 17 Message-ID: <16045760.1110411340848.JavaMail.root@ernie.psp.pas.earthlink.net> Hi Debbie, I'm a temp here at the Alaska Native Med. Center here in Anchorage, AK. They do Diff-Quik stains on all the Antral biopsies and when the Pathologist see that kind of epithelium. Mark Tarango ----- Original Message ----- From: "Trudgeon, Debbie" To: Sent: Tuesday, March 08, 2005 7:42 AM Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 14 Hi, About 3 weeks ago, I subscribed to Histonet and I submitted a question. I have not heard back or seen anything about it. Does this mean the moderator turned it down? The question was - What specimen sites are other labs running a Giemsa stain on? We currently run them daily on esophagus, stomach, and duodenum. Thanks, Debbie Debbie Trudgeon Charge Technologist Histology Royal Victoria Hospital 201 Georgian Drive Barrie, ON L4M 6M2 (705) 728-9090 x6446 trudgeond@rvh.on.ca From cfavara <@t> niaid.nih.gov Wed Mar 9 18:48:07 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IP 10 again Message-ID: I asked before and got no response, thought I would rty once more. Anyone doing any immunohistochemistry using anti-IP 10, any species frozen or paraffin? Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives From mayhew <@t> vaxxine.com Wed Mar 9 19:37:03 2005 From: mayhew <@t> vaxxine.com (D. Mayhew) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Giemsa stain for Helicobacter Message-ID: <003e01c52511$a9aa28e0$91e805d1@dave> Hi Debbie, We routinely use a Giemsa stain for Helicobacter on biopsies from anywhere in the stomach. Lee Mayhew MLT Niagara Health System St. Catharines General Site St. Catharines ON. From LuckG <@t> empirehealth.org Wed Mar 9 19:37:15 2005 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] 'CAP' regs web site/link Message-ID: Susan, Click on this link. www.cap.org/apps/docs/laboratory_accreditation/checklists/checklistftp.html Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Susan Owens [mailto:ohenry@dfw.net] Sent: Wednesday, March 09, 2005 2:29 PM To: Histonet Subject: [Histonet] 'CAP' regs web site/link Can anyone direct me to a web site where I can see the currant CAP regs???? Main point of interest is grossroom/cyrostat disinfection/cleaning. I did have some sites/links but they don't seem to be working. Susan Owens-TX ohenry@dfw.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.gorrie <@t> unsw.edu.au Wed Mar 9 19:44:54 2005 From: c.gorrie <@t> unsw.edu.au (Cath Gorrie) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] connector hose? In-Reply-To: Message-ID: Sorry, I sent this to the wrong list. Please ignore this message unless you happen to have one of these! Cath > Hi, > > Wondering of anybody can help me out before I go buying a new one of these. > > I'm looking for a connector hose from a nitrous oxide gas cylinder to a > halothan set-up. It's a blue hose with fittings on either end to attach to > regulator at the gas cylinder and a similar fitting on the other end. > > Maybe someone would have one of these laying around that they don't use > anymore. It may be quite generic in some labs, certainly not in ours. If you > have a spare and are willing to part with it, I'd really appreciate it. > > Regards, Cathy > > ---------------------------------------------------------------------------- > > Catherine Gorrie > School of Medical Sciences > University of New South Wales > Sydney, NSW > > ph: 02 9385 2462 > fax: 02 9385 8016 > e-mail: c.gorrie@unsw.edu.au > ---------------------------------------------------------------------------- > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katri <@t> cogeco.ca Wed Mar 9 20:27:08 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Commonly used stains for kidney disease progession?? References: <20050309211742.56409.qmail@web42301.mail.yahoo.com> Message-ID: <00b301c52518$a83e5fb0$6a9a9618@Katri> When we receive a native kidney biopsy (human), the routine is to cut serial sections at 3um and pick sections up on several slides. 3 or 4 get an H&E, 1 PAS, 1 Jones methenamine silver for basement membranes, 1 trichrome and 1 elastin stain ( we use Miller). Also one piece of the biopsy is snap frozen for consequent IF stains for immune complexes, and one piece is fixed for possible EM. There are many different kidney diseases and combination of all these stains will confirm or rule out many of them, but that is the pathologists' area of expertise. I know that for instance PAS and silver stains demonstrate the basement menbranes within the glomeruli and can indicate immune complex deposits within them anf if so the IF will determine what kind of immune complexes they are. These are just the routine stains, more are done if the pathologist requests. I think your pathologist is the best source of information in this area. Hope this helps a bit... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "- -" To: Sent: Wednesday, March 09, 2005 4:17 PM Subject: [Histonet] Commonly used stains for kidney disease progession?? > Hi everyone, > > Quick question: > > What are the commonly used stains/immunochemistry procedures to evaluate > progression of disease within kidneys? (I may need to know WHY also in > case I am not familiar with a certain technique, stain, or antibody) > > So far I have been told a number ranging from PAS, trichrome and a silver > technique - but I started to become confused. They all seem great but I > think I may need the WHY along with the WHAT when answers are being sent > back in reply. > > As usual, thank you for your assistance. > > G.T. Hebert > Cambridge Massachusetts > > > > > --------------------------------- > Celebrate Yahoo!'s 10th Birthday! > Yahoo! Netrospective: 100 Moments of the Web > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Mar 9 21:14:53 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] The Use of Placenta in the Understanding of Perinatal Injury Message-ID: A week. store, and divi Monday rolled around th disposed of. The refriger area. If there were any que placenta was available for testing. the abnormals, multiple births or any with quest Am looking f placentas. Is anyo institution. If for possible< thanks in advance Annette From dpconsult <@t> earthlink.net Thu Mar 10 05:44:45 2005 From: dpconsult <@t> earthlink.net (D Paulson [Source Medical Products]) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] NSH Self-Assessment Series CD - Cannot Install Message-ID: Has anyone had success installing this program? I have tried installing this on PC's with the following operating systems: Windows/XP Professional Windows/XP Home Windows 2000 Windows 98 I have the version that was released in December 2004. The contact at the NSH office said they personally had success using Windows/XP Professional but she acknowledged there are many people who have not been able to install it. This is the error message displayed initially: "The data for this program has been modified. The program will not start." then the install program attempts to install Microsoft Data Access Control (MDAC) version 2.5 For systems running Windows/XP you get a 'Fatal Setup Error' because Windows/XP includes MDAC with the operating system. The list of files on the CD in the 'Support' folder include: MDAC_TYP.DDF Date: 11/17/2004 Project1.DDF Date: 10/14/2004 Quiz.DDF Date: 01/29/2004 Quizbooks.DDF Date: 10/14/2004 SelfExam.exe Date: 11/17/2004 Start.exe Date: 11/17/2004 All the PC's have either Microsoft Office 2000/Professional or Microsoft Office 2003/Professional installed. Any insight you can give me would be appreciated. Thanks, Dick Paulson Source Medical Products dpconsult@earthlink.net Phone (800) 393-6345 From sagitalcuts <@t> yahoo.com Thu Mar 10 07:42:08 2005 From: sagitalcuts <@t> yahoo.com (Yolanda Maldonado) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC Validation on floating sections Message-ID: <20050310134209.11525.qmail@web53505.mail.yahoo.com> Hello Everyone: Can anyone tell me how to validate IHC on floating sections? Any input will be helpful Patricia Maldonado HT (ASCP) --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From Heather.A.Harper <@t> pcola.med.navy.mil Thu Mar 10 08:56:02 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Need Pay Info for VA hospitals or Naval Hospitals Message-ID: <807FE48C5A7CC940B973B58D32E7014318A76ADE@nhpens-exch1.pcola.med.navy.mil> Hi everyone, I just need information on pay grade. If you are a GS employee or contracter and work at a VA Hospital or a Naval Hospital and you gross, please let me know if you are a GS 7, 8 or 9. I'm about to write my own job description and I will be learning to gross so I need this information. Thanks in advance everyone and have a nice day. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL. From vazquezr <@t> ohsu.edu Thu Mar 10 09:02:57 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IP 10 again Message-ID: Cynthia, Sorry, but I don't. Responding just to let you know I am here and not ignoring your question. Robyn OHSU >>> "Favara, Cynthia (NIH/NIAID)" 03/09/05 4:48 PM >>> I asked before and got no response, thought I would rty once more. Anyone doing any immunohistochemistry using anti-IP 10, any species frozen or paraffin? Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Mar 10 09:14:30 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Need Pay Info for VA hospitals or Naval Hospitals Message-ID: Aren't all Federal job descriptions for GS ratings the same across the board? Heather.A.Harper@pcola.med.navy.mil Sent by: histonet-bounces@lists.utsouthwestern.edu 03/10/2005 08:56 AM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Need Pay Info for VA hospitals or Naval Hospitals Hi everyone, I just need information on pay grade. If you are a GS employee or contracter and work at a VA Hospital or a Naval Hospital and you gross, please let me know if you are a GS 7, 8 or 9. I'm about to write my own job description and I will be learning to gross so I need this information. Thanks in advance everyone and have a nice day. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Mar 10 10:04:43 2005 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Cell block preparation for paraffin embedding Message-ID: All, Would someone be willing to share their procedure for preparing cell blocks (from cell cultures) for processing to paraffin blocks. I haven't done this in ages...do you still use gelatin? What do you use to keep the cells in the cassettes? A colleague needs some guidance pretty much from square one. Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From Diane.Gladney <@t> se.amedd.army.mil Thu Mar 10 10:34:28 2005 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Need Pay Info for VA hospitals or Naval Hospitals Message-ID: <4D55B2E997EFAE4DA6081DDE100B830237CF46@amedmlsermc133.amed.ds.army.mil> No they are not!! Trust me on this one. Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology/Cytology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart Avenue P.O. Box 484 Ft. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, March 10, 2005 10:15 AM To: Heather.A.Harper@pcola.med.navy.mil Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need Pay Info for VA hospitals or Naval Hospitals Aren't all Federal job descriptions for GS ratings the same across the board? Heather.A.Harper@pcola.med.navy.mil Sent by: histonet-bounces@lists.utsouthwestern.edu 03/10/2005 08:56 AM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Need Pay Info for VA hospitals or Naval Hospitals Hi everyone, I just need information on pay grade. If you are a GS employee or contracter and work at a VA Hospital or a Naval Hospital and you gross, please let me know if you are a GS 7, 8 or 9. I'm about to write my own job description and I will be learning to gross so I need this information. Thanks in advance everyone and have a nice day. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Mar 10 10:43:28 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Fatty tissues processing Message-ID: I have previously asked for assistance in a processing schedule anyone may have for processing colons, lymph nodes, lipomas, breast tissues, and any "fatty" tissue that is difficult to process on a schedule with your routine tissue and biopsy schedules. I received a couple of responses, but, one of them has a time allotment that is not within the parameters that I have to work with. Is there anyone out there that has had some GREAT results and would you please share that by Emailing me or addressing it on the histonet? Thanks SO MUCH ahead of time!! You "histonetters" are the greatest!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From tpmorken <@t> labvision.com Thu Mar 10 10:53:48 2005 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Survey of microscopes used Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CAB4@usca0082k08.labvision.apogent.com> Histonetters, Would people please reply to me privately about which brand and model of microscope are favored by your general pathologists? I'm interested in bight field only, not fluorescent or specialized microsopes. Thanks! Tim Morken Product Development Lab Vision - Neomarkers From TillRenee <@t> uams.edu Thu Mar 10 11:05:42 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] cell surface antigens Message-ID: When staining for cell surface antigens, is there a difference between cyrosections or FFPE? Or would it depend on the particular antibody? Also, with antigen retrieval, have you noticed any difference between solutions and pH? Renee' Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tpmorken <@t> labvision.com Thu Mar 10 11:09:30 2005 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC Validation on floating sections Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CAB8@usca0082k08.labvision.apogent.com> Yolanda, Compare the floating section IHC to paraffin or frozen section IHC (whichever applies) in a parallel study. If the results are the same, it validates the floating section method. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yolanda Maldonado Sent: Thursday, March 10, 2005 5:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation on floating sections Hello Everyone: Can anyone tell me how to validate IHC on floating sections? Any input will be helpful Patricia Maldonado HT (ASCP) --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Thu Mar 10 11:11:58 2005 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR)) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Need Pay Info for VA hospitals or Naval Hospitals Message-ID: <8F3AB322628548428A992EFB0E80F5D315D6306F@nihexchange8.nih.gov> That's exactly right they are not. They can put anything on them they want to. Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Drug Discovery Pain Branch > ---------- > From: Gladney, Diane C Ms MACH > Sent: Thursday, March 10, 2005 10:34 AM > To: Jackie M O'Connor; Heather.A.Harper@pcola.med.navy.mil > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Need Pay Info for VA hospitals or Naval > Hospitals > > No they are not!! Trust me on this one. > > Diane > > Diane C. Gladney, HT (ASCP) > Supervisor, Histology/Cytology > Moncrief Army Community Hospital > Dept. of Pathology > 4500 Stuart Avenue > P.O. Box 484 > Ft. Jackson, SC 29207 > > Email: diane.gladney@se.amedd.army.mil > Phone: 803-751-2530 FAX: 803-751-7829 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M > O'Connor > Sent: Thursday, March 10, 2005 10:15 AM > To: Heather.A.Harper@pcola.med.navy.mil > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Need Pay Info for VA hospitals or Naval > Hospitals > > Aren't all Federal job descriptions for GS ratings the same across the > board? > > > > > > Heather.A.Harper@pcola.med.navy.mil > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/10/2005 08:56 AM > > > To: histonet@lists.utsouthwestern.edu > cc: > Subject: [Histonet] Need Pay Info for VA hospitals or > Naval Hospitals > > > Hi everyone, > > I just need information on pay grade. If you are a GS employee > or > contracter and work at a VA Hospital or a Naval Hospital and you gross, > please let me know if you are a GS 7, 8 or 9. I'm about to write my own > > job > description and I will be learning to gross so I need this information. > Thanks in advance everyone and have a nice day. > > > > Heather A. Harper > > Supervisor of Histology/Morgue > > Naval Hospital of Pensacola, FL. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Tom_Wells <@t> bcit.ca Thu Mar 10 11:30:55 2005 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Fri Sep 16 15:24:45 2005 Subject: *****SPAM***** [Histonet] NSH Self-Assessment Series CD - Cannot Install In-Reply-To: Message-ID: I have exactly the same problem. There is supposed to be a replacement cd available, but, so far I haven't received it. If you hear anything please let me know. Tom From Vickroy.Jim <@t> mhsil.com Thu Mar 10 11:43:30 2005 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] FDA statement for ASR antibodies used in immunohistochemistry Message-ID: When using the FDA disclaimer in surgical reports when using an FDA disclaimer are people using their own lab name in the disclaimer statement (since we now have to validate the use of ASR's and RUO's), or are they using the vendor's name of the company that developed the antibody, such as Cell Marque, Ventana, BD, etc. Jim Vickroy Memorial Medical Center From ahenn <@t> vaccinex.com Thu Mar 10 11:52:33 2005 From: ahenn <@t> vaccinex.com (Alicia Henn Ph.D.) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Re: false positive kidney References: <200503101706.j2AH6VC07813@nexserverpro.vaccinex.com> Message-ID: <002a01c52599$f02dbb00$2c01a8c0@alicia> Hi Jackie, We struggled with similar background problems staining human kidney and liver. Blocking didn't help. We switched to the Dako Envision polymer-based system for detection, which is biotin-free. Problems solved. Obi-wan. Now that's a name I've not heard in a long time... Alicia Henn, Ph.D. Vaccinex, Inc. Date: Wed, 9 Mar 2005 16:32:48 -0600 From: "Jackie M O'Connor" Subject: [Histonet] It's a mystery . . . To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetskis - Maybe I'm thinking too hard - I can't seem to get rid of false positive staining in negative control sections of human and mouse kidney human and pancreas tumor. I'm using EDTA HIER (for the first time), in an SABC protocol with a Goat Pab. I've tried two different (vendor) Rab anti Goat biotinylated secondarys. I'm using an avidin/biotin block - each for 20 minutes, (two different lots/vendors) but I'm still getting this false staining in the negative (omitted primary) control sections. Is it possible to not be able to block ALL biotin? I've never seen this before. I can't imagine what else I'm seeing - but like I said - my brain is tired. Before I switch to an HRP conjugated secondary - can anyone suggest what else this could be? I know EDTA can cause background, but there is no primary on these sections. Will someone loan me their brain? I can't help but wonder if the EDTA is causing my mystery - but I have to use it. Help me, Obi-wan-kenobi. Jackie O' From tpmorken <@t> labvision.com Thu Mar 10 11:59:57 2005 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] FDA statement for ASR antibodies used in immunohis tochemistry Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CABC@usca0082k08.labvision.apogent.com> Jim, you should put in the name of the lab that validates the antibody, not the name of the company you buy it from. The ASR is, by definition, not validated by the vendor, so the vendor cannot be named as the lab that "... developed..." and determined the "...performance characteristics..." that lead to validation. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, March 10, 2005 9:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FDA statement for ASR antibodies used in immunohistochemistry When using the FDA disclaimer in surgical reports when using an FDA disclaimer are people using their own lab name in the disclaimer statement (since we now have to validate the use of ASR's and RUO's), or are they using the vendor's name of the company that developed the antibody, such as Cell Marque, Ventana, BD, etc. Jim Vickroy Memorial Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Thu Mar 10 12:24:22 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Fatty tissues processing Message-ID: <20050310182422.63542.qmail@web30409.mail.mud.yahoo.com> Hi Dorothy, I am sure you will responses from people who know a lot more about such protocols than I do. I will give you a pathologists point of view. First, whoever is doing your grossing has to cut these tissues no more than 3 mm thick across the entire piece. If you are dealing with residents this is a common problem. From the edge it looks 3 mm but at the center it is thicker. I tell my gang to try for 2-3 mm with fatty tissues. Once cut the proper thickness, it must fix in a proper quantity of formalin for more time than it sits in the processor. I would make sure after cutting the 3mm section, most fatty tissues spend at least a 3 hours more in formalin before putting it in the processor. In the case of breast biopsies there is too much at risk in trying to read a poorly processed section. We have gone to letting most breast specimens fix overnight before processing. But it still has to be cut thin prior to fixing or you will be wasting your time. Rather than have special processing protocols for fatty tissue, you may find better results by simplymaking sure your grossing people taking proper care in sectioning and fixation of the tissues. Stephen Peters MD Pathology Innovations From akbitting <@t> geisinger.edu Thu Mar 10 12:29:53 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] FDA statement for ASR antibodies used in immunohistochemistry Message-ID: Univ. of Pittsburgh and Geisinger use our own names. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Vickroy, Jim" 03/10/05 12:43 PM >>> When using the FDA disclaimer in surgical reports when using an FDA disclaimer are people using their own lab name in the disclaimer statement (since we now have to validate the use of ASR's and RUO's), or are they using the vendor's name of the company that developed the antibody, such as Cell Marque, Ventana, BD, etc. Jim Vickroy Memorial Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From mtitford <@t> usouthal.edu Thu Mar 10 10:36:45 2005 From: mtitford <@t> usouthal.edu (Michael Titford) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Placenta anyone? Message-ID: Jennifer McDonald asks about placenta. We routinely exam every placenta from every baby born at out Children's & Woman's hospital. Normal placenta receive "gross only" treatment of weights, dimensions and description. Abnormal history or findings trigger a "gross in" of four blocks containing proximal, medial and distal cord, membranes, full thickness and sample of any pathological areas found. Abnormal history includes history of premature birth, abruption, history of more than one spontaneous abortion, overweight baby, etc, etc. I think examining placenta is now pretty much the standard of care. OBGYN people like documentation of placenta from problem births in case a case goes to court Mike Titford USA Pathology Mobile AL USA. From gcallis <@t> montana.edu Thu Mar 10 12:59:27 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] cell surface antigens In-Reply-To: References: Message-ID: <6.0.0.22.1.20050310115721.01b8f2c8@gemini.msu.montana.edu> Renee, For what species, human or murine? At 10:05 AM 3/10/2005, you wrote: >When staining for cell surface antigens, is there a difference between >cyrosections or FFPE? Or would it depend on the particular antibody? >Also, with antigen retrieval, have you noticed any difference between >solutions and pH? > > > >Renee' > > >Confidentiality Notice: This e-mail message, including any attachments, is >for the sole use of the intended recipient(s) and may contain confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, please >contact the sender by reply e-mail and destroy all copies of the original >message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From ktournear <@t> cox.net Thu Mar 10 13:29:17 2005 From: ktournear <@t> cox.net (Kim Tournear) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Updating my address book Message-ID: <14830568.1110482957989.JavaMail.Administrator@win01> Hi I am creating an address book that keeps all my contacts updated. I would like to include you in it. Please use the link below to enter your details for me: http://www.bebo.com/fr1/10343558a511515716b145692819c478583130d18 We will exchange our contact information and every time we make changes they will automatically update in our address books. Thank You! Kim From kerry.l.crabb <@t> gsk.com Thu Mar 10 14:07:02 2005 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] NSH Self Assessment Series CD Message-ID: NSH did have some defective CDs in the Self Assessment series. These were found after selling them during the S/C in Toronto. Most if not all of them have been replaced with a new copy. Additional problems have been found with some of the new CDs. NSH is working with the vendor to identify the source of these problems and how it will be fixed. If you have a problem with your CD, please contact the NSH office via email at "histo@nsh.org". The NSH office with communicate with you about a fix when the problem is resolved. From jhofecker <@t> yahoo.com Thu Mar 10 14:14:09 2005 From: jhofecker <@t> yahoo.com (Jennifer Hofecker) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Tau antibody Message-ID: <20050310201409.37564.qmail@web21002.mail.yahoo.com> Hi everyone, Are any of you currently using Tau from Dako? What detection system are you using? I Would appreciate all positive/negative input regarding this antibody. Also, has another supplier's Tau proven better in your experience? Thanks in advance, Jennifer Hofecker __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From JMacDonald <@t> mtsac.edu Thu Mar 10 15:05:37 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC humidity chamber? In-Reply-To: Message-ID: Ted Pella has Immunostain Moisture Chambers. They hold 10 slides. We are using them in our student IHC labs. They are working well for us. The catalog number is 21049 and list price is $43.00. See www.tedpella.com Jennifer MacDonald From JNocito <@t> Pathreflab.com Thu Mar 10 16:45:47 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] FDA statement for ASR antibodies used inimmunohistochemistry In-Reply-To: Message-ID: Jim, we just had a CAP inspection and the inspector was looking just for that statement. We have our lab name in the disclaimer because we were the ones that performed all the testing. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Thursday, March 10, 2005 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FDA statement for ASR antibodies used inimmunohistochemistry When using the FDA disclaimer in surgical reports when using an FDA disclaimer are people using their own lab name in the disclaimer statement (since we now have to validate the use of ASR's and RUO's), or are they using the vendor's name of the company that developed the antibody, such as Cell Marque, Ventana, BD, etc. Jim Vickroy Memorial Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Mar 10 17:15:04 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC humidity chamber? In-Reply-To: References: Message-ID: <6.0.0.22.1.20050310161125.01b54a48@gemini.msu.montana.edu> These are identical to the ones Evergreen Scientific makes and sells only their prices are 3 boxes/pkg and you pay approx $80 for the pkg of 3 boxes, considerably cheaper unless Evergreen has changed their pricing in recent times. I wonder if Evergreen is the manufacturer of these boxes for Ted Pella? They look exactly the same. 02:05 PM 3/10/2005, you wrote: >Ted Pella has Immunostain Moisture Chambers. They hold 10 slides. We are >using them in our student IHC labs. They are working well for us. The >catalog number is 21049 and list price is $43.00. See www.tedpella.com >Jennifer MacDonald >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From mab70 <@t> medschl.cam.ac.uk Fri Mar 11 02:41:49 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Adipose tissue Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211149F@mius2.medlan.cam.ac.uk> Hi Histonetters, Like Dorothy Webb, I too would like to receive advice about a good process for adipose tissue. In my case, I need good sections of adipose tissue itself. My process isn't too bad but I would like to improve it as I get breaks in some the cell boundaries and would like to ensure the best possible preparation. I am particularly interested in what clearing agents are recommended and if anyone has a preference as to which make of wax they like. I have to say that I am not keen on using xylene as clearing agent since it seems to harden some of my other tissues too much. I would like to use the same reagents for all my samples, even if I have to use different timings for different batches of processing, I can set up to 10 programmes on my processor (Leica TP1020, 12 stations, all with vacuum, ambient temperature except for the wax stations of course.) Thanks a lot. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR From JEllin <@t> yumaregional.org Fri Mar 11 06:53:01 2005 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC with decal Message-ID: I am wondering what people are using for a decal solution that does not interfere with IHC staining. ?? All the Help would greatly be appreciated. Jesus Ellin Yuma Regional Medical Center From miffordclark <@t> optonline.net Fri Mar 11 07:25:09 2005 From: miffordclark <@t> optonline.net (clifford berger) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC with decal References: Message-ID: <000601c5263d$bf499be0$0300a8c0@dellovo0ll7kuk> Any of our decalicifiers work fine. You should take a look at our website, www.decal-bone.com. The technical reprints and links page is filled with articles about IHC after decalcification. You can also call us for free samples. Best regards, Cliff Berger Decal Chemical Corporation 1-800-428-5856 www.decal-bone.com ----- Original Message ----- From: "Jesus Ellin" To: Sent: Friday, March 11, 2005 7:53 AM Subject: [Histonet] IHC with decal >I am wondering what people are using for a decal solution that does not >interfere with IHC staining. ?? All the Help would greatly be >appreciated. > > Jesus Ellin > Yuma Regional Medical Center > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wood <@t> dcpah.msu.edu Fri Mar 11 07:40:51 2005 From: wood <@t> dcpah.msu.edu (Thomas Wood) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC Decal Message-ID: We use Decal II from Surgipath and it does a descent job. RDO I have little luck. -Tom Wood Michigan State University From cgfields <@t> lexhealth.org Fri Mar 11 08:18:16 2005 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC with decal Message-ID: We use Rapid-Cal Immuno from BBC. It is working fine. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center W. Columbia, SC -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, March 11, 2005 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC with decal I am wondering what people are using for a decal solution that does not interfere with IHC staining. ?? All the Help would greatly be appreciated. Jesus Ellin Yuma Regional Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From emanzano <@t> eoh.pitt.edu Fri Mar 11 08:57:37 2005 From: emanzano <@t> eoh.pitt.edu (Lopez Manzano, Elisenda) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Question about markers for Graw Factor Antibody Message-ID: <30B8F0F69E08A9428D634ED8866334DC1F6222@eohserver01.eoh.pitt.edu> Hi histonetters, I am currently working with paraffin wax embedded mouse embryos and I am looking for markers for Graw Factor Antibody acn. I've found some interesting ones in my bibliography, however I couldn't find these companies on the web: - Oncogene - Signal transduction Does anybody know any official website or can anybody help me finding the price of these products? From: Oncogene - TGF Reference - GF 10 - TGF-R Reference - ms129091 From: Signal Transduction - EGF-R Reference 2232 - TGF-R1 Reference 3712 Thanks! Elisenda Lopez-Manzano From LettJ <@t> ent.wustl.edu Fri Mar 11 09:24:13 2005 From: LettJ <@t> ent.wustl.edu (Lett, Jaclynn) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Reichert Histostat manual Message-ID: <8153997545DC1A4DB92AE75F5DC34500CA0C9F@EXCHANGE.wusm-pcf.wustl.edu> Hello, We recently inherited a Reichert Histostat 855 made by Cambridge (the manufacturer's label also says "975CJ Cryostat Microtome"). Its operation manual could not be found. Does anyone have a manual and would you be willing to send us a copy? It would be greatly appreciated. Also, does anyone know of a disposable knife holder suitable for this model? The steel knife holder in this machine clamps the knife on its left side. I hope someone can assist us. Thank you so much, Jaclynn Lett Senior Research Technician Electron Microscopy Core Facility Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 Voice: 747-7257 Fax: 747-7230 Email: lettj@ent.wustl.edu
The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From ploykasek <@t> phenopath.com Fri Mar 11 10:17:07 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Placenta anyone? In-Reply-To: Message-ID: When I was working at a large community hospital in the South, all placentas were examined. For placentas of routine births an "embed & hold" was done. Briefly, this consisted of doing a gross exam, cutting in 2-3 blocks, processing these, & embedding the blocks. These blocks were not cut unless a physician called & requested it. For non-routine births, the placentas were grossed, cut, processed, sectioned & stained. We rarely froze any tissue. Usually the placenta would be examined in a group once or twice a week. They were held in formalin in a fridge until examined. This type of placenta service sure seemed to make the ob/gyn types very happy. Patti Loykasek PhenoPath Laboratories > Jennifer McDonald asks about placenta. > We routinely exam every placenta from every baby born at out Children's > & Woman's hospital. Normal placenta receive "gross only" treatment of > weights, dimensions and description. > Abnormal history or findings trigger a "gross in" of four blocks > containing proximal, medial and distal cord, membranes, full thickness > and sample of any pathological areas found. Abnormal history includes > history of premature birth, abruption, history of more than one > spontaneous abortion, overweight baby, etc, etc. > I think examining placenta is now pretty much the standard of care. > OBGYN people like documentation of placenta from problem births in case > a case goes to court > > Mike Titford > USA Pathology > Mobile AL USA. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Don.Birgerson <@t> leica-microsystems.com Fri Mar 11 10:18:17 2005 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Reichert Histostat manual Message-ID: Hi Jaclynn, I will E mail a PDF copy of the operating instructions under separate cover. If you have further questions, please phone me. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From settembr <@t> umdnj.edu Fri Mar 11 10:23:17 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Tau antibody Message-ID: Hello Jennifer, I use Tau from Dako with their detection kit LSAB2. But I have used other kits in the past that work too. Vectors ABC kit and I am sure many others work as well. We use it with an Alzheimer's brain control with no pretreatment. I use it at a 1:800 dilution incubated for 15 minutes. Good Luck, Dana Settembre University Hospital - UMDNJ Newark, NJ USA >>> Jennifer Hofecker 3/10/2005 3:14:09 PM >>> Hi everyone, Are any of you currently using Tau from Dako? What detection system are you using? I Would appreciate all positive/negative input regarding this antibody. Also, has another supplier's Tau proven better in your experience? Thanks in advance, Jennifer Hofecker __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From n.cragg <@t> epistem.co.uk Fri Mar 11 10:22:08 2005 From: n.cragg <@t> epistem.co.uk (ncragg) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] F4/80 on human tumour xenografts in mouse Message-ID: <01C52656.79AB2770.n.cragg@epistem.co.uk> Hello, Has anyone tried murine F4/80 IHC staining on human tumour xenografts grown in nude mice to look for infiltrating macrophages? We are using the Caltag rat monoclonal, BM8, although we've got it working nicely on normal mouse spleen, the staining pattern is unusual in the tumour xenografts, staining is seen around the human tumour cells (almost like tumour matrix) rather than infiltrating mouse cells. Has anyone seen this pattern before or can suggest what is happening here? Thank you in advance, Nicola Nicola Cragg BSc Epistem Ltd Manchester, UK From pruegg <@t> ihctech.net Fri Mar 11 10:36:34 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] IHC with decal In-Reply-To: Message-ID: <200503111636.j2BGaVdf026361@chip.viawest.net> I use Immunocal from Decal Chemicals or make my own 5% Formic Acid. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole Fields Sent: Friday, March 11, 2005 7:18 AM To: 'Jesus Ellin' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC with decal We use Rapid-Cal Immuno from BBC. It is working fine. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center W. Columbia, SC -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, March 11, 2005 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC with decal I am wondering what people are using for a decal solution that does not interfere with IHC staining. ?? All the Help would greatly be appreciated. Jesus Ellin Yuma Regional Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rolmsche <@t> unlnotes.unl.edu Fri Mar 11 11:38:55 2005 From: rolmsche <@t> unlnotes.unl.edu (Robin Olmscheid) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] workshops Message-ID: <4.1.20050311113302.00b01578@unlnotes01.unl.edu> I would like to know if there are any special stains workshops to attend. I know several years ago there was a week long workshop for learning and doing special stains. This week long workshop took place some where in the south, maybe Georgia. If anyone has any information concerning this please let me know. Thanks. From BoozerKA <@t> pa1.ah.org Fri Mar 11 11:51:12 2005 From: BoozerKA <@t> pa1.ah.org (Kathleen Boozer) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Prion Lab Message-ID: Is there a prion referral lab in the NW (Oregon)? From dlcowie <@t> prodigy.net Fri Mar 11 11:59:17 2005 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] exhausting tissue blocks Message-ID: <20050311175917.2794.qmail@web81003.mail.yahoo.com> hello histonetters, I was wondering what thoughts any of you have on the subject of cutting away and exhausting tissue blocks when doing recuts. We often get requests from our pathologists to cut deepers and exhaust the block ie: exhaust in 3 slides of deepers. Does anyone know of any reg's that cover this kind of thing? It really goes against the grain to cut away all the tissue. Plus, we are supposed to keep the blocks for 10 years. It defeats the purpose of doing so. Any response would be appreciated. Thanks, Dawn Cowie, HT From la.sebree <@t> hosp.wisc.edu Fri Mar 11 12:05:24 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] workshops Message-ID: Robin, Why don't you check into your next state society meeting and if nothing is lined up yet, suggest a special stain workshop be considered. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robin Olmscheid Sent: Friday, March 11, 2005 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] workshops I would like to know if there are any special stains workshops to attend. I know several years ago there was a week long workshop for learning and doing special stains. This week long workshop took place some where in the south, maybe Georgia. If anyone has any information concerning this please let me know. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From info <@t> instrumedics.com Fri Mar 11 12:11:23 2005 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] question Message-ID: <004601c52665$bf887270$6401a8c0@INSTRUMEDICS22> Does anyone know how I can reach Liz Chilpaia who is with Premier Histology Laboratory in Colorado. Noone picks up the phone at the lab. Bernice Schiller From Charles.Embrey <@t> carle.com Fri Mar 11 12:21:54 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] exhausting tissue blocks Message-ID: Have you expressed your concerns to the pathologists? Too many times I've seen histotechs timid about an open dialog with their pathologists. In this case the reason is simple to the pathologist. I have had reason to exhaust all tissue many times through my career. CAP says that you have to retain the blocks; it doesn't say you have to keep tissue in them. If the pathologist is looking for a small focus of malignancy and it hasn't shown up on the slides he has but is found later on deeper cuts he could be sued. As lawsuits are becoming more numerous pathologists are becoming more "gun shy" about leaving unexamined tissue within the block especially when the case is clinically highly suspicious for disease and none is found in the slides examined. Keeping the exhausted block for 10 years proves that no tissue remains and the slides the diagnosis was made from are also available. Charles Embrey Pathologists' Assistant Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Cowie Sent: Friday, March 11, 2005 11:59 AM To: histonet Subject: [Histonet] exhausting tissue blocks hello histonetters, I was wondering what thoughts any of you have on the subject of cutting away and exhausting tissue blocks when doing recuts. We often get requests from our pathologists to cut deepers and exhaust the block ie: exhaust in 3 slides of deepers. Does anyone know of any reg's that cover this kind of thing? It really goes against the grain to cut away all the tissue. Plus, we are supposed to keep the blocks for 10 years. It defeats the purpose of doing so. Any response would be appreciated. Thanks, Dawn Cowie, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Fri Mar 11 12:24:16 2005 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Thanks for the information on the Decal Message-ID: Thank you all for the information that you gave me on the decal, now my next question is how long to you let bone cores, bones from the joints, and other bone items sit for decal??? Jesus Ellin Yuma Regional Medical Center From gcallis <@t> montana.edu Fri Mar 11 12:37:51 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:45 2005 Subject: Question on Re: [Histonet] IHC Decal In-Reply-To: References: Message-ID: <6.0.0.22.1.20050311113612.01b698b0@gemini.msu.montana.edu> What does Decal II from Surgipath contain? Formic acid? Is it buffered with sodium citrate or sodium formate. RDO has a very high concentration of hydrochloric acid plus some additives - would be considered a rapid decalcifier. At 06:40 AM 3/11/2005, you wrote: > We use Decal II from Surgipath and it does a descent job. RDO I > have little luck. > >-Tom Wood >Michigan State University > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From SCheasty <@t> memorialcare.org Fri Mar 11 12:43:13 2005 From: SCheasty <@t> memorialcare.org (Sandy Cheasty) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Microwave GMS Problem Solved... Thanks! Message-ID: <59FE4C55358C6A42B84BAEC6092CEDBC05ACF5CE@sbnt7> Thanks to all who gave me suggestions... and the winner is: 1. Chromic acid 10 min room temp (any brand) 2. Tap H2O, rinse well 3. Sodium bisulfite 1 min (any brand) 4. Tap H2O, rinse well 5. DI water x 3 changes 6. SIGMA meth silver reagent (1 vial makes 100 ml, that will last at least a week if you don't add the Borax. We use 25 ml at a time and add 1 ml of SIGMA Borax) Place slides in microwave proof slide holder, zap in MW at full power in 15 seconds bursts to a maximum of 3 bursts. After each burst pour the reagent back and forth into another acid clean container to distribute the heat layers in the solution and check the control for adequate development. 7. Tap H2O, rinse well 8. DI water x 2-3 changes 9. Gold chloride 10. yadda 11. yadda 12. yadda 13. Coverslip The fungal and pneumocytis organism staining is thorough, delicate, fast and without background. Just like mother to used make. I tried this with Rowley reagents and Polyscientific reagents, the pneumocystis didn't stain well and the background was butt-ugly. Sandy ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Sign up for your free MemorialCare Medical Information and Access Card at http://www.memorialcare.com/apps/AccessCard/AboutCard.cfm From gcallis <@t> montana.edu Fri Mar 11 12:50:53 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:45 2005 Subject: [Histonet] Reichert Histostat manual In-Reply-To: <8153997545DC1A4DB92AE75F5DC34500CA0C9F@EXCHANGE.wusm-pcf.w ustl.edu> References: <8153997545DC1A4DB92AE75F5DC34500CA0C9F@EXCHANGE.wusm-pcf.wustl.edu> Message-ID: <6.0.0.22.1.20050311113937.01b54c60@gemini.msu.montana.edu> Jaclynn, The disposable knife holder that works in this old cryostat is the one invented for use with an AO 820 microtome many, many years ago. It is a solid holder with a preset angle that needs no adjustment. You can slip a high profile blade (only profile blade you can use in this holder) using a side release lever. The joy is holder bottom parts fit into the slots on this cryostat, and will lock securely into place. You have to play with picking up sections a bit but it works. There may some of these old disposable knife holders floating around storage rooms and someone willing to give one to you. Sorry, our manual is long gone but contact Don.Birgerson@leica-microsystems.com - he is a great source of info for these old instruments. One thing, keep all sliding parts well oiled with cryostat oil in this machine is a must but it will work. That side arm knife holder was a horrible monster to use, and not the safest design either. At 08:24 AM 3/11/2005, you wrote: >Hello, > >We recently inherited a Reichert Histostat 855 made by Cambridge (the >manufacturer's label also says "975CJ Cryostat Microtome"). Its >operation manual could not be found. Does anyone have a manual and >would you be willing to send us a copy? It would be greatly >appreciated. > >Also, does anyone know of a disposable knife holder suitable for this >model? The steel knife holder in this machine clamps the knife on its >left side. > >I hope someone can assist us. > >Thank you so much, > >Jaclynn Lett > >Senior Research Technician >Electron Microscopy Core Facility >Department of Otolaryngology >Washington University School of Medicine >660 S. Euclid Ave., Campus Box 8115 >St. Louis, MO 63110 > >Voice: 747-7257 >Fax: 747-7230 >Email: lettj@ent.wustl.edu > > >
The materials in this message are private and may contain Protected >Healthcare Information. If you are not the intended recipient, be advised >that any unauthorized use, disclosure, copying or the taking of any action >in reliance on the contents of this information is strictly prohibited. If >you have received this email in error, please immediately notify the >sender via telephone or return mail. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Dorothy.L.Webb <@t> HealthPartners.Com Fri Mar 11 12:53:31 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Decals for IHC Message-ID: We use Rapid Cal Immuno from BBC for our decals and it works great and is wonderful with the IHC's! It was a fairly recent switch and improved our quality. They are very good at giving you a sample. Contact Adrain Biesecker at 1-800-635-4477. Good luck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, March 11, 2005 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fatty tissues processing (Stephen Peters M.D.) 2. Re: FDA statement for ASR antibodies used in immunohistochemistry (Angela Bitting) 3. Placenta anyone? (Michael Titford) 4. Re: cell surface antigens (Gayle Callis) 5. Updating my address book (Kim Tournear) 6. NSH Self Assessment Series CD (kerry.l.crabb@gsk.com) 7. Tau antibody (Jennifer Hofecker) 8. RE: IHC humidity chamber? (Jennifer MacDonald) 9. RE: FDA statement for ASR antibodies used inimmunohistochemistry (Joe Nocito) 10. RE: IHC humidity chamber? (Gayle Callis) 11. Adipose tissue (Margaret Blount) 12. IHC with decal (Jesus Ellin) 13. Re: IHC with decal (clifford berger) 14. IHC Decal (Thomas Wood) 15. RE: IHC with decal (Carole Fields) 16. Question about markers for Graw Factor Antibody (Lopez Manzano, Elisenda) 17. Reichert Histostat manual (Lett, Jaclynn) 18. Re: Placenta anyone? (Patti Loykasek) 19. Re: Reichert Histostat manual (Don.Birgerson@leica-microsystems.com) 20. Re: Tau antibody (Dana Settembre) 21. F4/80 on human tumour xenografts in mouse (ncragg) 22. RE: IHC with decal (Patsy Ruegg) 23. workshops (Robin Olmscheid) 24. Prion Lab (Kathleen Boozer) 25. exhausting tissue blocks (Dawn Cowie) ---------------------------------------------------------------------- Message: 1 Date: Thu, 10 Mar 2005 10:24:22 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] Fatty tissues processing To: histonet@lists.utsouthwestern.edu Message-ID: <20050310182422.63542.qmail@web30409.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Dorothy, I am sure you will responses from people who know a lot more about such protocols than I do. I will give you a pathologists point of view. First, whoever is doing your grossing has to cut these tissues no more than 3 mm thick across the entire piece. If you are dealing with residents this is a common problem. From the edge it looks 3 mm but at the center it is thicker. I tell my gang to try for 2-3 mm with fatty tissues. Once cut the proper thickness, it must fix in a proper quantity of formalin for more time than it sits in the processor. I would make sure after cutting the 3mm section, most fatty tissues spend at least a 3 hours more in formalin before putting it in the processor. In the case of breast biopsies there is too much at risk in trying to read a poorly processed section. We have gone to letting most breast specimens fix overnight before processing. But it still has to be cut thin prior to fixing or you will be wasting your time. Rather than have special processing protocols for fatty tissue, you may find better results by simplymaking sure your grossing people taking proper care in sectioning and fixation of the tissues. Stephen Peters MD Pathology Innovations ------------------------------ Message: 2 Date: Thu, 10 Mar 2005 13:29:53 -0500 From: "Angela Bitting" Subject: Re: [Histonet] FDA statement for ASR antibodies used in immunohistochemistry To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Univ. of Pittsburgh and Geisinger use our own names. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Vickroy, Jim" 03/10/05 12:43 PM >>> When using the FDA disclaimer in surgical reports when using an FDA disclaimer are people using their own lab name in the disclaimer statement (since we now have to validate the use of ASR's and RUO's), or are they using the vendor's name of the company that developed the antibody, such as Cell Marque, Ventana, BD, etc. Jim Vickroy Memorial Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ------------------------------ Message: 3 Date: Thu, 10 Mar 2005 10:36:45 -0600 From: "Michael Titford" Subject: [Histonet] Placenta anyone? To: Message-ID: Content-Type: text/plain; charset=US-ASCII Jennifer McDonald asks about placenta. We routinely exam every placenta from every baby born at out Children's & Woman's hospital. Normal placenta receive "gross only" treatment of weights, dimensions and description. Abnormal history or findings trigger a "gross in" of four blocks containing proximal, medial and distal cord, membranes, full thickness and sample of any pathological areas found. Abnormal history includes history of premature birth, abruption, history of more than one spontaneous abortion, overweight baby, etc, etc. I think examining placenta is now pretty much the standard of care. OBGYN people like documentation of placenta from problem births in case a case goes to court Mike Titford USA Pathology Mobile AL USA. ------------------------------ Message: 4 Date: Thu, 10 Mar 2005 11:59:27 -0700 From: Gayle Callis Subject: Re: [Histonet] cell surface antigens To: "Till, Renee" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050310115721.01b8f2c8@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Renee, For what species, human or murine? At 10:05 AM 3/10/2005, you wrote: >When staining for cell surface antigens, is there a difference between >cyrosections or FFPE? Or would it depend on the particular antibody? >Also, with antigen retrieval, have you noticed any difference between >solutions and pH? > > > >Renee' > > >Confidentiality Notice: This e-mail message, including any attachments, >is >for the sole use of the intended recipient(s) and may contain confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, please >contact the sender by reply e-mail and destroy all copies of the original >message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 5 Date: Thu, 10 Mar 2005 19:29:17 +0000 (GMT) From: Kim Tournear Subject: [Histonet] Updating my address book To: histonet@pathology.swmed.edu Message-ID: <14830568.1110482957989.JavaMail.Administrator@win01> Content-Type: text/plain; charset=us-ascii Hi I am creating an address book that keeps all my contacts updated. I would like to include you in it. Please use the link below to enter your details for me: http://www.bebo.com/fr1/10343558a511515716b145692819c478583130d18 We will exchange our contact information and every time we make changes they will automatically update in our address books. Thank You! Kim ------------------------------ Message: 6 Date: Thu, 10 Mar 2005 15:07:02 -0500 From: kerry.l.crabb@gsk.com Subject: [Histonet] NSH Self Assessment Series CD To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii NSH did have some defective CDs in the Self Assessment series. These were found after selling them during the S/C in Toronto. Most if not all of them have been replaced with a new copy. Additional problems have been found with some of the new CDs. NSH is working with the vendor to identify the source of these problems and how it will be fixed. If you have a problem with your CD, please contact the NSH office via email at "histo@nsh.org". The NSH office with communicate with you about a fix when the problem is resolved. ------------------------------ Message: 7 Date: Thu, 10 Mar 2005 12:14:09 -0800 (PST) From: Jennifer Hofecker Subject: [Histonet] Tau antibody To: histonet@lists.utsouthwestern.edu Message-ID: <20050310201409.37564.qmail@web21002.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi everyone, Are any of you currently using Tau from Dako? What detection system are you using? I Would appreciate all positive/negative input regarding this antibody. Also, has another supplier's Tau proven better in your experience? Thanks in advance, Jennifer Hofecker __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ ------------------------------ Message: 8 Date: Thu, 10 Mar 2005 13:05:37 -0800 From: Jennifer MacDonald Subject: RE: [Histonet] IHC humidity chamber? To: Dana Settembre Cc: Histonet@lists.utsouthwestern.edu, JOHN.PHILLIPS@new-tr.wales.nhs.uk, histonet-bounces@lists.utsouthwestern.edu, kosmicdog@hotmail.com Message-ID: Content-Type: text/plain; charset="US-ASCII" Ted Pella has Immunostain Moisture Chambers. They hold 10 slides. We are using them in our student IHC labs. They are working well for us. The catalog number is 21049 and list price is $43.00. See www.tedpella.com Jennifer MacDonald ------------------------------ Message: 9 Date: Thu, 10 Mar 2005 16:45:47 -0600 From: "Joe Nocito" Subject: RE: [Histonet] FDA statement for ASR antibodies used inimmunohistochemistry To: "Vickroy, Jim" , Message-ID: Content-Type: text/plain; charset="us-ascii" Jim, we just had a CAP inspection and the inspector was looking just for that statement. We have our lab name in the disclaimer because we were the ones that performed all the testing. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Thursday, March 10, 2005 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FDA statement for ASR antibodies used inimmunohistochemistry When using the FDA disclaimer in surgical reports when using an FDA disclaimer are people using their own lab name in the disclaimer statement (since we now have to validate the use of ASR's and RUO's), or are they using the vendor's name of the company that developed the antibody, such as Cell Marque, Ventana, BD, etc. Jim Vickroy Memorial Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 10 Mar 2005 16:15:04 -0700 From: Gayle Callis Subject: RE: [Histonet] IHC humidity chamber? To: Jennifer MacDonald , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050310161125.01b54a48@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed These are identical to the ones Evergreen Scientific makes and sells only their prices are 3 boxes/pkg and you pay approx $80 for the pkg of 3 boxes, considerably cheaper unless Evergreen has changed their pricing in recent times. I wonder if Evergreen is the manufacturer of these boxes for Ted Pella? They look exactly the same. 02:05 PM 3/10/2005, you wrote: >Ted Pella has Immunostain Moisture Chambers. They hold 10 slides. We >are using them in our student IHC labs. They are working well for us. >The catalog number is 21049 and list price is $43.00. See >www.tedpella.com Jennifer MacDonald >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 11 Date: Fri, 11 Mar 2005 08:41:49 -0000 From: Margaret Blount Subject: [Histonet] Adipose tissue To: "Histonet (E-mail)" Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211149F@mius2.medlan.cam.ac.uk> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, Like Dorothy Webb, I too would like to receive advice about a good process for adipose tissue. In my case, I need good sections of adipose tissue itself. My process isn't too bad but I would like to improve it as I get breaks in some the cell boundaries and would like to ensure the best possible preparation. I am particularly interested in what clearing agents are recommended and if anyone has a preference as to which make of wax they like. I have to say that I am not keen on using xylene as clearing agent since it seems to harden some of my other tissues too much. I would like to use the same reagents for all my samples, even if I have to use different timings for different batches of processing, I can set up to 10 programmes on my processor (Leica TP1020, 12 stations, all with vacuum, ambient temperature except for the wax stations of course.) Thanks a lot. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR ------------------------------ Message: 12 Date: Fri, 11 Mar 2005 05:53:01 -0700 From: "Jesus Ellin" Subject: [Histonet] IHC with decal To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii I am wondering what people are using for a decal solution that does not interfere with IHC staining. ?? All the Help would greatly be appreciated. Jesus Ellin Yuma Regional Medical Center ------------------------------ Message: 13 Date: Fri, 11 Mar 2005 08:25:09 -0500 From: clifford berger Subject: Re: [Histonet] IHC with decal To: Jesus Ellin , histonet@lists.utsouthwestern.edu Message-ID: <000601c5263d$bf499be0$0300a8c0@dellovo0ll7kuk> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original Any of our decalicifiers work fine. You should take a look at our website, www.decal-bone.com. The technical reprints and links page is filled with articles about IHC after decalcification. You can also call us for free samples. Best regards, Cliff Berger Decal Chemical Corporation 1-800-428-5856 www.decal-bone.com ----- Original Message ----- From: "Jesus Ellin" To: Sent: Friday, March 11, 2005 7:53 AM Subject: [Histonet] IHC with decal >I am wondering what people are using for a decal solution that does not >interfere with IHC staining. ?? All the Help would greatly be >appreciated. > > Jesus Ellin > Yuma Regional Medical Center > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 11 Mar 2005 08:40:51 -0500 From: "Thomas Wood" Subject: [Histonet] IHC Decal To: Message-ID: Content-Type: text/plain; charset=US-ASCII We use Decal II from Surgipath and it does a descent job. RDO I have little luck. -Tom Wood Michigan State University ------------------------------ Message: 15 Date: Fri, 11 Mar 2005 09:18:16 -0500 From: Carole Fields Subject: RE: [Histonet] IHC with decal To: 'Jesus Ellin' Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use Rapid-Cal Immuno from BBC. It is working fine. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center W. Columbia, SC -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, March 11, 2005 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC with decal I am wondering what people are using for a decal solution that does not interfere with IHC staining. ?? All the Help would greatly be appreciated. Jesus Ellin Yuma Regional Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. ------------------------------ Message: 16 Date: Fri, 11 Mar 2005 09:57:37 -0500 From: "Lopez Manzano, Elisenda" Subject: [Histonet] Question about markers for Graw Factor Antibody To: Message-ID: <30B8F0F69E08A9428D634ED8866334DC1F6222@eohserver01.eoh.pitt.edu> Content-Type: text/plain; charset="utf-8" Hi histonetters, I am currently working with paraffin wax embedded mouse embryos and I am looking for markers for Graw Factor Antibody acn. I've found some interesting ones in my bibliography, however I couldn't find these companies on the web: - Oncogene - Signal transduction Does anybody know any official website or can anybody help me finding the price of these products? From: Oncogene - TGF Reference - GF 10 - TGF-R Reference - ms129091 From: Signal Transduction - EGF-R Reference 2232 - TGF-R1 Reference 3712 Thanks! Elisenda Lopez-Manzano ------------------------------ Message: 17 Date: Fri, 11 Mar 2005 09:24:13 -0600 From: "Lett, Jaclynn" Subject: [Histonet] Reichert Histostat manual To: Message-ID: <8153997545DC1A4DB92AE75F5DC34500CA0C9F@EXCHANGE.wusm-pcf.wustl.edu> Content-Type: text/plain; charset="us-ascii" Hello, We recently inherited a Reichert Histostat 855 made by Cambridge (the manufacturer's label also says "975CJ Cryostat Microtome"). Its operation manual could not be found. Does anyone have a manual and would you be willing to send us a copy? It would be greatly appreciated. Also, does anyone know of a disposable knife holder suitable for this model? The steel knife holder in this machine clamps the knife on its left side. I hope someone can assist us. Thank you so much, Jaclynn Lett Senior Research Technician Electron Microscopy Core Facility Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 Voice: 747-7257 Fax: 747-7230 Email: lettj@ent.wustl.edu
The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ------------------------------ Message: 18 Date: Fri, 11 Mar 2005 08:17:07 -0800 From: Patti Loykasek Subject: Re: [Histonet] Placenta anyone? To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" When I was working at a large community hospital in the South, all placentas were examined. For placentas of routine births an "embed & hold" was done. Briefly, this consisted of doing a gross exam, cutting in 2-3 blocks, processing these, & embedding the blocks. These blocks were not cut unless a physician called & requested it. For non-routine births, the placentas were grossed, cut, processed, sectioned & stained. We rarely froze any tissue. Usually the placenta would be examined in a group once or twice a week. They were held in formalin in a fridge until examined. This type of placenta service sure seemed to make the ob/gyn types very happy. Patti Loykasek PhenoPath Laboratories > Jennifer McDonald asks about placenta. > We routinely exam every placenta from every baby born at out > Children's & Woman's hospital. Normal placenta receive "gross only" > treatment of weights, dimensions and description. Abnormal history or > findings trigger a "gross in" of four blocks containing proximal, > medial and distal cord, membranes, full thickness and sample of any > pathological areas found. Abnormal history includes history of > premature birth, abruption, history of more than one spontaneous > abortion, overweight baby, etc, etc. I think examining placenta is now > pretty much the standard of care. OBGYN people like documentation of > placenta from problem births in case a case goes to court > > Mike Titford > USA Pathology > Mobile AL USA. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 11 Mar 2005 10:18:17 -0600 From: Don.Birgerson@leica-microsystems.com Subject: Re: [Histonet] Reichert Histostat manual To: "Lett, Jaclynn" Cc: histonet-bounces@lists.utsouthwestern.edu, histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Jaclynn, I will E mail a PDF copy of the operating instructions under separate cover. If you have further questions, please phone me. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 20 Date: Fri, 11 Mar 2005 11:23:17 -0500 From: Dana Settembre Subject: Re: [Histonet] Tau antibody To: histonet@lists.utsouthwestern.edu, jhofecker@yahoo.com Message-ID: Content-Type: text/plain; charset=US-ASCII Hello Jennifer, I use Tau from Dako with their detection kit LSAB2. But I have used other kits in the past that work too. Vectors ABC kit and I am sure many others work as well. We use it with an Alzheimer's brain control with no pretreatment. I use it at a 1:800 dilution incubated for 15 minutes. Good Luck, Dana Settembre University Hospital - UMDNJ Newark, NJ USA >>> Jennifer Hofecker 3/10/2005 3:14:09 PM >>> Hi everyone, Are any of you currently using Tau from Dako? What detection system are you using? I Would appreciate all positive/negative input regarding this antibody. Also, has another supplier's Tau proven better in your experience? Thanks in advance, Jennifer Hofecker __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Fri, 11 Mar 2005 16:22:08 -0000 From: ncragg Subject: [Histonet] F4/80 on human tumour xenografts in mouse To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <01C52656.79AB2770.n.cragg@epistem.co.uk> Content-Type: text/plain; charset="us-ascii" Hello, Has anyone tried murine F4/80 IHC staining on human tumour xenografts grown in nude mice to look for infiltrating macrophages? We are using the Caltag rat monoclonal, BM8, although we've got it working nicely on normal mouse spleen, the staining pattern is unusual in the tumour xenografts, staining is seen around the human tumour cells (almost like tumour matrix) rather than infiltrating mouse cells. Has anyone seen this pattern before or can suggest what is happening here? Thank you in advance, Nicola Nicola Cragg BSc Epistem Ltd Manchester, UK ------------------------------ Message: 22 Date: Fri, 11 Mar 2005 09:36:34 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] IHC with decal To: "'Carole Fields'" , "'Jesus Ellin'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <200503111636.j2BGaVdf026361@chip.viawest.net> Content-Type: text/plain; charset="us-ascii" I use Immunocal from Decal Chemicals or make my own 5% Formic Acid. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole Fields Sent: Friday, March 11, 2005 7:18 AM To: 'Jesus Ellin' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC with decal We use Rapid-Cal Immuno from BBC. It is working fine. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center W. Columbia, SC -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, March 11, 2005 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC with decal I am wondering what people are using for a decal solution that does not interfere with IHC staining. ?? All the Help would greatly be appreciated. Jesus Ellin Yuma Regional Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Fri, 11 Mar 2005 11:38:55 -0600 From: Robin Olmscheid Subject: [Histonet] workshops To: histonet@lists.utsouthwestern.edu Message-ID: <4.1.20050311113302.00b01578@unlnotes01.unl.edu> Content-Type: text/plain; charset="us-ascii" I would like to know if there are any special stains workshops to attend. I know several years ago there was a week long workshop for learning and doing special stains. This week long workshop took place some where in the south, maybe Georgia. If anyone has any information concerning this please let me know. Thanks. ------------------------------ Message: 24 Date: Fri, 11 Mar 2005 09:51:12 -0800 From: "Kathleen Boozer" Subject: [Histonet] Prion Lab To: Message-ID: Content-Type: text/plain; charset=US-ASCII Is there a prion referral lab in the NW (Oregon)? ------------------------------ Message: 25 Date: Fri, 11 Mar 2005 09:59:17 -0800 (PST) From: Dawn Cowie Subject: [Histonet] exhausting tissue blocks To: histonet Message-ID: <20050311175917.2794.qmail@web81003.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii hello histonetters, I was wondering what thoughts any of you have on the subject of cutting away and exhausting tissue blocks when doing recuts. We often get requests from our pathologists to cut deepers and exhaust the block ie: exhaust in 3 slides of deepers. Does anyone know of any reg's that cover this kind of thing? It really goes against the grain to cut away all the tissue. Plus, we are supposed to keep the blocks for 10 years. It defeats the purpose of doing so. Any response would be appreciated. Thanks, Dawn Cowie, HT ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 21 **************************************** ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From csawrenk <@t> bccancer.bc.ca Fri Mar 11 12:53:40 2005 From: csawrenk <@t> bccancer.bc.ca (Sawrenko, Christina) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] MSH2 rabbit polyclonal antibody from Oncogene Message-ID: <57AD92D1EDD1854BAAC02DD7DDAF0E3601700567@srvex10.phsabc.ehcnet.ca> Good morning all! Does anyone have experience with this antibody? We are experiencing a cytoplasmic "blush" in addition to very nice nuclear staining in a tumor with a MLH1 mutation. In order to achieve nice nuclear staining, we are using DAKO high pH 9.9 for heat mediated antigen retrieval. We use EnVision+ as the detection system. This blush may be OK so are asking for other's experience. Thanks for any help you can give. Chris Sawrenko Histopathology BC Cancer Agency Vancouver, BC Canada From sharon.osborn <@t> dnax.org Fri Mar 11 13:09:33 2005 From: sharon.osborn <@t> dnax.org (Osborn, Sharon) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] RE: special stain workshops Message-ID: <29B25753F6B1D51196110002A589D44402397F18@PALMSG30.us.schp.com> Robin, Lamar Jones has conducted week long special stain workshops. I do not have his direct information; you probably can find him on the NSH listing. Sharon Osborn DNAX Palo Alto, CA Date: Fri, 11 Mar 2005 11:38:55 -0600 From: Robin Olmscheid Subject: [Histonet] workshops To: histonet@lists.utsouthwestern.edu Message-ID: <4.1.20050311113302.00b01578@unlnotes01.unl.edu> Content-Type: text/plain; charset="us-ascii" I would like to know if there are any special stains workshops to attend. I know several years ago there was a week long workshop for learning and doing special stains. This week long workshop took place some where in the south, maybe Georgia. If anyone has any information concerning this please let me know. Thanks. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From Dorothy.L.Webb <@t> HealthPartners.Com Fri Mar 11 13:17:21 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Nails and decal for immunos Message-ID: We use a product called "Tween" that works great, but, gentle on nails and does not grow fungus as it sits around like Nair can. If you are interested in it, Email me or call at 651-254-2962 and I will give you the details. We use RapidCal Immuno from BBc Biochemical and have had very good results. They will be happy to send you a sample, just contact Adrian Biesecker @1-800-635-4477. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From MAUGER <@t> email.chop.edu Fri Mar 11 13:26:59 2005 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] bile pigment Message-ID: Hi Everyone, Does anyone know of a way to remove bile pigment from tissue sections? I have been searching, and can't find any methods. Thanks, Jo >>> "Sandy Cheasty" 03/11/05 1:43 PM >>> Thanks to all who gave me suggestions... and the winner is: 1. Chromic acid 10 min room temp (any brand) 2. Tap H2O, rinse well 3. Sodium bisulfite 1 min (any brand) 4. Tap H2O, rinse well 5. DI water x 3 changes 6. SIGMA meth silver reagent (1 vial makes 100 ml, that will last at least a week if you don't add the Borax. We use 25 ml at a time and add 1 ml of SIGMA Borax) Place slides in microwave proof slide holder, zap in MW at full power in 15 seconds bursts to a maximum of 3 bursts. After each burst pour the reagent back and forth into another acid clean container to distribute the heat layers in the solution and check the control for adequate development. 7. Tap H2O, rinse well 8. DI water x 2-3 changes 9. Gold chloride 10. yadda 11. yadda 12. yadda 13. Coverslip The fungal and pneumocytis organism staining is thorough, delicate, fast and without background. Just like mother to used make. I tried this with Rowley reagents and Polyscientific reagents, the pneumocystis didn't stain well and the background was butt-ugly. Sandy ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Sign up for your free MemorialCare Medical Information and Access Card at http://www.memorialcare.com/apps/AccessCard/AboutCard.cfm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Mar 11 13:45:02 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Thanks for the information on the Decal In-Reply-To: References: Message-ID: <6.0.0.22.1.20050311123740.01b8e968@gemini.msu.montana.edu> We do a decalcification endpoint test to know when the calcium is removed from any large bones, however a Jamshidi needle biopsy may require only a few hours with an acid decalcifier. There is a chemical test one can do - simple and cheap. An exact time for decalcification really can't be given, but if you let bone sit in acids (formic or hydrochloric) too long, then you will damage tissue, cells, and stainabitily, and in particular immunostaining. this is often referred to as overdecalcification but a better term is overexposure to acid. That is why endpoint testing is used - to control decalcification. At 11:24 AM 3/11/2005, you wrote: >Thank you all for the information that you gave me on the decal, now my >next question is how long to you let bone cores, bones from the joints, >and other bone items sit for decal??? > >Jesus Ellin >Yuma Regional Medical Center > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From LettJ <@t> ent.wustl.edu Fri Mar 11 13:51:37 2005 From: LettJ <@t> ent.wustl.edu (Lett, Jaclynn) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Reichert Histostat manual Message-ID: <8153997545DC1A4DB92AE75F5DC34500CA0CA1@EXCHANGE.wusm-pcf.wustl.edu> Gayle, Thank you for replying. Don Birgerson has already contacted me about the manual and will send a PDF file. Here's hoping that someone on the list has one of those disposable knife holders and no longer needs it. Maybe we can make a deal. Thank you, Jaclynn Lett Senior Research Technician Electron Microscopy Core Facility Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 >Voice: 747-7257 Fax: 747-7230 >Email: lettj@ent.wustl.edu > -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Friday, March 11, 2005 12:51 PM To: Lett, Jaclynn; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reichert Histostat manual Jaclynn, The disposable knife holder that works in this old cryostat is the one invented for use with an AO 820 microtome many, many years ago. It is a solid holder with a preset angle that needs no adjustment. You can slip a high profile blade (only profile blade you can use in this holder) using a side release lever. The joy is holder bottom parts fit into the slots on this cryostat, and will lock securely into place. You have to play with picking up sections a bit but it works. There may some of these old disposable knife holders floating around storage rooms and someone willing to give one to you. Sorry, our manual is long gone but contact Don.Birgerson@leica-microsystems.com - he is a great source of info for these old instruments. One thing, keep all sliding parts well oiled with cryostat oil in this machine is a must but it will work. That side arm knife holder was a horrible monster to use, and not the safest design either. At 08:24 AM 3/11/2005, you wrote: >Hello, > >We recently inherited a Reichert Histostat 855 made by Cambridge (the >manufacturer's label also says "975CJ Cryostat Microtome"). Its >operation manual could not be found. Does anyone have a manual and >would you be willing to send us a copy? It would be greatly >appreciated. > >Also, does anyone know of a disposable knife holder suitable for this >model? The steel knife holder in this machine clamps the knife on its >left side. > >I hope someone can assist us. > >Thank you so much, > >Jaclynn Lett > >Senior Research Technician >Electron Microscopy Core Facility >Department of Otolaryngology >Washington University School of Medicine >660 S. Euclid Ave., Campus Box 8115 >St. Louis, MO 63110 > >Voice: 747-7257 >Fax: 747-7230 >Email: lettj@ent.wustl.edu > > >
The materials in this message are private and may contain Protected >Healthcare Information. If you are not the intended recipient, be advised >that any unauthorized use, disclosure, copying or the taking of any action >in reliance on the contents of this information is strictly prohibited. If >you have received this email in error, please immediately notify the >sender via telephone or return mail. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX)
The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From JWEEMS <@t> sjha.org Fri Mar 11 15:25:25 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Reichert Histostat manual Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA4563D@sjhaexc02.sjha.org> Klaus Dern has disposable blade holders that fit in a regular blade holder. You might want to contact him - 706-635-8840. They set in the blade holder and tighten when you clamp it down just as you would a blade. Hope this helps. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lett, Jaclynn Sent: Friday, March 11, 2005 2:52 PM To: Gayle Callis; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reichert Histostat manual Gayle, Thank you for replying. Don Birgerson has already contacted me about the manual and will send a PDF file. Here's hoping that someone on the list has one of those disposable knife holders and no longer needs it. Maybe we can make a deal. Thank you, Jaclynn Lett Senior Research Technician Electron Microscopy Core Facility Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 >Voice: 747-7257 Fax: 747-7230 >Email: lettj@ent.wustl.edu > -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Friday, March 11, 2005 12:51 PM To: Lett, Jaclynn; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reichert Histostat manual Jaclynn, The disposable knife holder that works in this old cryostat is the one invented for use with an AO 820 microtome many, many years ago. It is a solid holder with a preset angle that needs no adjustment. You can slip a high profile blade (only profile blade you can use in this holder) using a side release lever. The joy is holder bottom parts fit into the slots on this cryostat, and will lock securely into place. You have to play with picking up sections a bit but it works. There may some of these old disposable knife holders floating around storage rooms and someone willing to give one to you. Sorry, our manual is long gone but contact Don.Birgerson@leica-microsystems.com - he is a great source of info for these old instruments. One thing, keep all sliding parts well oiled with cryostat oil in this machine is a must but it will work. That side arm knife holder was a horrible monster to use, and not the safest design either. At 08:24 AM 3/11/2005, you wrote: >Hello, > >We recently inherited a Reichert Histostat 855 made by Cambridge (the >manufacturer's label also says "975CJ Cryostat Microtome"). Its >operation manual could not be found. Does anyone have a manual and >would you be willing to send us a copy? It would be greatly >appreciated. > >Also, does anyone know of a disposable knife holder suitable for this >model? The steel knife holder in this machine clamps the knife on its >left side. > >I hope someone can assist us. > >Thank you so much, > >Jaclynn Lett > >Senior Research Technician >Electron Microscopy Core Facility >Department of Otolaryngology >Washington University School of Medicine >660 S. Euclid Ave., Campus Box 8115 >St. Louis, MO 63110 > >Voice: 747-7257 >Fax: 747-7230 >Email: lettj@ent.wustl.edu > > >
The materials in this message are private and may contain Protected >Healthcare Information. If you are not the intended recipient, be advised >that any unauthorized use, disclosure, copying or the taking of any action >in reliance on the contents of this information is strictly prohibited. If >you have received this email in error, please immediately notify the >sender via telephone or return mail. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX)
The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From bucana <@t> audumla.mdacc.tmc.edu Fri Mar 11 19:28:17 2005 From: bucana <@t> audumla.mdacc.tmc.edu (Corazon D. Bucana) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] F4/80 on human tumour xenografts in mouse In-Reply-To: <01C52656.79AB2770.n.cragg@epistem.co.uk> Message-ID: <5.1.1.6.0.20050311192433.023cdaf0@audumla.mdacc.tmc.edu> We published a paper in 1992 entitled: Different patterns of macrophage infiltration into allogeneic-murine and xenogeneic-human neoplasms growing in nude mice. Bucana et al. Am J Pathol 1992 Nov : 141(5):1225-36. At 04:22 PM 3/11/2005 +0000, you wrote: >Hello, > >Has anyone tried murine F4/80 IHC staining on human tumour xenografts grown >in nude mice to look for infiltrating macrophages? We are using the Caltag >rat monoclonal, BM8, although we've got it working nicely on normal mouse >spleen, the staining pattern is unusual in the tumour xenografts, staining >is seen around the human tumour cells (almost like tumour matrix) rather >than infiltrating mouse cells. Has anyone seen this pattern before or can >suggest what is happening here? > >Thank you in advance, > >Nicola > >Nicola Cragg BSc >Epistem Ltd >Manchester, UK > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BoozerKA <@t> pa1.ah.org Sun Mar 13 09:58:54 2005 From: BoozerKA <@t> pa1.ah.org (Kathleen Boozer) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Transporting legs from surgery Message-ID: We double bag but the bone still comes through occasionally. Any ideas on how to get from 2nd floor to first? Any commercial aid available? Thanks! From msdfloyd <@t> worldnet.att.net Sun Mar 13 19:53:05 2005 From: msdfloyd <@t> worldnet.att.net (Dina Floyd) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Transporting legs from surgery References: Message-ID: <000501c52838$91a75770$8cea400c@yourxhtr8hvc4p> We have a large bio hazard transport box we use. It is designed for this specific purpose. ----- Original Message ----- From: "Kathleen Boozer" To: Sent: Sunday, March 13, 2005 9:58 AM Subject: [Histonet] Transporting legs from surgery > We double bag but the bone still comes through occasionally. Any ideas > on how to get from 2nd floor to first? Any commercial aid available? > Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Mon Mar 14 03:27:09 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Tunel - Katy Whalley Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114A2@mius2.medlan.cam.ac.uk> Dear Katy, some time ago you offered to send out your protocol for the Roche Tunel kit to another member of histonet. I wonder if you would mind sharing it with me please. I have tried it in the past and got very much too much staining in spite of the controls working as they should. Thanks MargaretMargaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR From ajennings <@t> unmc.edu Mon Mar 14 08:09:28 2005 From: ajennings <@t> unmc.edu (ajennings@unmc.edu) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Question about markers for Graw Factor Antibody In-Reply-To: <30B8F0F69E08A9428D634ED8866334DC1F6222@eohserver01.eoh.pitt.edu> Message-ID: Oncogene is now part of Calbiochem, which is part of the EMD Bioscience family..... try here http://www.emdbiosciences.com/html/cbc/home.html and you wonder why you couldn't find it :-) Signal Transduction as a company name? I am not familiar with that one. "Lopez Manzano, Elisenda" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Question about markers for Graw Factor Antibody 03/11/2005 08:57 AM Hi histonetters, I am currently working with paraffin wax embedded mouse embryos and I am looking for markers for Graw Factor Antibody acn. I've found some interesting ones in my bibliography, however I couldn't find these companies on the web: - Oncogene - Signal transduction Does anybody know any official website or can anybody help me finding the price of these products? From: Oncogene - TGF Reference - GF 10 - TGF-R Reference - ms129091 From: Signal Transduction - EGF-R Reference 2232 - TGF-R1 Reference 3712 Thanks! Elisenda Lopez-Manzano _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Mon Mar 14 10:24:10 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] LCM in bone tissues Message-ID: <20050314162411.48885.qmail@web15507.mail.cnb.yahoo.com> Hi, histonetters, At present I am doing my experiments in bone metabolism. I plan to abtain osteoblasts or chondrocytes from bone and cartilage tissues, but I do not have some experience in LCM, so I hope that you can do me a favor . Thank you! Qian --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From gcallis <@t> montana.edu Mon Mar 14 10:48:26 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Transporting legs from surgery In-Reply-To: References: Message-ID: <6.0.0.22.1.20050314094246.01b7b6c8@gemini.msu.montana.edu> The doubling of biohazard bags is an excellent suggestion, but get heavy duty bags. If you could convince whoever is bagging the limbs, to wrap a cloth towel or heavy duty guaze around a jagged bone end before bagging to prevent puncturing the plastic bags. At 08:58 AM 3/13/2005, you wrote: >We double bag but the bone still comes through occasionally. Any ideas >on how to get from 2nd floor to first? Any commercial aid available? >Thanks! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Allison_Scott <@t> hchd.tmc.edu Mon Mar 14 12:11:38 2005 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Control Blocks Message-ID: I am in desperate need of a "PCP" control block. If any one has one to spare, I will be eternally grateful. We have a hard time getting controls these days. I am willing to share what control blocks that I have. Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Dept. of Pathology 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From relia1 <@t> earthlink.net Mon Mar 14 12:32:10 2005 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Relias Histology Job Opportunity Update 3/14/05 Message-ID: Hello Histonetters, My name is Pam Barker and I am a recruiter that has specialized in permanent placement in the histology profession for the past 2 ? years. All of the positions I work with are fulltime 40 hour per week positions with top hospitals, labs and doctors offices. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance and in some cases sign on bonuses. My services are FREE of charge to you. All of my fees are paid by my clients, (the facilities that I represent). I represent companies nationwide that are in need of histology supervisors, histotechnologists and histo technicians. Here is a list of my most exciting current openings: 1. Histopathology Supervisor ? Minnesota 2. Histo Tech ? Minnesota 3. Histo Tech ? Florida (current Florida license required) 4. Histo Tech ? Indiana 5. Histo Tech ? Illinois 6. Histo Tech ? Washington If you are interested in any of these positions please call me at 866-607-3542 or e-mail me at relia1@earthlink.net If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Also if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. Thank you, Pam ? 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From anthony.rosalia <@t> citigroup.com Mon Mar 14 12:52:31 2005 From: anthony.rosalia <@t> citigroup.com (Rosalia, Anthony [IIG]) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] IHC stainer Message-ID: I am trying to get feedback on the current options for IHC stainers. I am familiar with Ventana, Biogenex, and Dako products on paper but would love to get some feedback on their clinical performance to aid in decision making process. Thanks in advance. Anthony Rosalia (212)643-5884 From kelly.mcqueeney <@t> bms.com Mon Mar 14 13:06:29 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Anyone use the Fisher Microprobe Staining system? Message-ID: <4235E0B5.6010606@bms.com> Hi Histonetters, Has anyone used the Fisher Microprobe Staining system? Do you have any problems? We are interested in a very simple staining system for only one 60-minute incubation in hot ligand. I have used the Thermo Sequenza but we are having technical issues, plus loading the slides is a pain. We do not work with more than 20 slides at a time (and using radioligand, clean-up is an issue). I would appreciate no response from reps concerning complex, automated systems, we have already determined they are not right for us. Thanks, Kelly From pruegg <@t> ihctech.net Mon Mar 14 14:31:37 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] chrondrocytes and sub-chondral bone images Message-ID: <200503142031.j2EKVUdf028214@chip.viawest.net> Please could anyone direct me to, or share some digital micrographs showing chondrocytes and sub-chondral bone. Thank you, Patsy From anthony.rosalia <@t> citigroup.com Mon Mar 14 14:51:14 2005 From: anthony.rosalia <@t> citigroup.com (Rosalia, Anthony [IIG]) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] H&E Staining Message-ID: As of today H&E staining is a manual process in our lab. Would love to hear any feedback on automating this process. I run one busy practice, and one I would consider pretty small. Thanks, Anthony Rosalia (212)643-5884 From HornHV <@t> archildrens.org Mon Mar 14 15:23:25 2005 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] H&E Staining Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6322BAB3@EMAIL.archildrens.org> We have the Shandon Gemini and we really like it. It has a small footprint, is fairly easy to use and has been dependable, not without a few problems but nothing major. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital - Changing Children's Lives Phone - 501.364.4240 Fax - 501.364.3912 Visit us on the web at www. archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosalia, Anthony [IIG] Sent: Monday, March 14, 2005 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Staining As of today H&E staining is a manual process in our lab. Would love to hear any feedback on automating this process. I run one busy practice, and one I would consider pretty small. Thanks, Anthony Rosalia (212)643-5884 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From ynwang <@t> u.washington.edu Mon Mar 14 15:38:52 2005 From: ynwang <@t> u.washington.edu (Y. Wang) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] thought regarding transporting limbs Message-ID: I just had a thought regarding the transporting of limbs and the problem of bones sticking through the bags. I don't know if it would be suitable, but they make rubbish bags now that are meant to be un-pierceable (I remember seeing images of someone putting sharp twigs and boxes without having anything poking through) . Could you use two of these with some biohazard tape/label? Or one of these inside a biohaz bag? Yak-Nam From pruegg <@t> ihctech.net Mon Mar 14 17:15:10 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] seeking contract lab for histomorphometry Message-ID: <200503142315.j2ENF2df026939@chip.viawest.net> Anyone doing histomorphometry bone formation measurements on a contract basis? I have a customer doing a study with bone implants. I am preparing GMA sections for him without decalcification. He has fluorochrome (tetracyline) labelled the animals and needs to have the bone formation measurements between the two labels. I am sure he wants to measure osteoid and count osteoblasts and osteoclasts as well. I can do the sectioning but not equipted with an Image Analysis System to do the measurements. Patsy Ruegg From ploykasek <@t> phenopath.com Mon Mar 14 17:51:27 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] H&E Staining In-Reply-To: Message-ID: We are using a Sakura stainer. We do H&E's, deparaffinize/peroxidase block our immunos, & counterstain our immunos on it. I like the fact that it can run different programs at the same time. It has a good throughput, too. Please contact me if you need more specifics.> Patti Loykasek PhenoPath Laboratories Seattle, WA As of today H&E staining is a manual process in our lab. Would love to hear > any feedback on automating this process. I run one busy practice, and one I > would consider pretty small. > > Thanks, > Anthony Rosalia > (212)643-5884 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Mar 14 18:17:28 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] seeking contract lab for histomorphometry In-Reply-To: <200503142315.j2ENF2df026939@chip.viawest.net> References: <200503142315.j2ENF2df026939@chip.viawest.net> Message-ID: <6.0.0.22.1.20050314171605.01b4e878@gemini.msu.montana.edu> Patsy, Go on the web and type in bone histomorphometry, you will get several hits on first try for contract labs. At 04:15 PM 3/14/2005, you wrote: >Anyone doing histomorphometry bone formation measurements on a contract >basis? I have a customer doing a study with bone implants. I am preparing >GMA sections for him without decalcification. He has fluorochrome >(tetracyline) labelled the animals and needs to have the bone formation >measurements between the two labels. I am sure he wants to measure osteoid >and count osteoblasts and osteoclasts as well. I can do the sectioning but >not equipted with an Image Analysis System to do the measurements. > >Patsy Ruegg >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Laurie.Pereira <@t> sdcounty.ca.gov Mon Mar 14 18:30:01 2005 From: Laurie.Pereira <@t> sdcounty.ca.gov (Pereira, Laurie ) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Veterinary Pathologist wanted Message-ID: Hey all, Our laboratory is looking for a veterinary pathologist. We are located in beautiful San Diego, California. Please see the attached job posting. Thank you for your attention. <> Laurie L. Pereira, RVT SDCADDL (County Veterinarian) 5555 Overland Avenue Suite 4103 San Diego, CA. 92123 Phone: 858-694-3384 Fax: 858-571-4268 From Laurie.Pereira <@t> sdcounty.ca.gov Mon Mar 14 18:54:29 2005 From: Laurie.Pereira <@t> sdcounty.ca.gov (Pereira, Laurie ) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Veterinary Pathologist wanted Message-ID: Hey all, I tried this e-mail earlier but the attachment didn't attach:) Here we go again! Position Announcement: Veterinary Pathologist Veterinary Pathologist. The San Diego County Animal Disease Diagnostic Laboratory welcomes applicants for a full time position in a growing, dynamic, veterinary diagnostic laboratory. This County-sponsored laboratory diagnoses diseases in animals from veterinarians, citizens, the County Department of Animal Services, research institutions, biotech companies, wildlife groups, CA Fish and Game, USDA, U.S. Navy, universities and other organizations. It is also the West Coast Study Site for the C.L. Davis Foundation. In addition to necropsy and biopsy exams, the Veterinary Pathologist also provides educational seminars to the community and may hold adjunct professor status at area universities. We are looking for an innovative, forward-thinking individual who works well with others. The laboratory is centrally located in San Diego, with convenient access to freeways, shopping, restaurants, attractive communities, and beaches. Depending on qualifications, a salary of $89,377 to $108,659 is offered, with full medical, vacation and retirement benefits. For more information, please see , or contact Dr. Nikos Gurfield at (858) 694-8973, nikos.gurfield@sdcounty.ca.gov. Laurie L. Pereira, RVT SDCADDL (County Veterinarian) 5555 Overland Avenue Suite 4103 San Diego, CA. 92123 Phone: 858-694-3384 Fax: 858-571-4268 From CrochiereSteve <@t> aol.com Mon Mar 14 20:55:24 2005 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Legs from OR Message-ID: <1c8.247e95e6.2f67a89c@aol.com> Ours are delivered to the morgue cooler and the paperwork delivered to the lab by the OR staff. The legs are wrapped in absorbent pads (usually 2 or 3) and the double bagged. I haven't seen one bust out of the bag in the at least 4 years. Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 From paw555 <@t> yahoo.com Mon Mar 14 21:12:48 2005 From: paw555 <@t> yahoo.com (pam plumlee) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Cutting frozen fat Message-ID: <20050315031249.44880.qmail@web11603.mail.yahoo.com> Hi all: I have a project that the researcher wants frozen sections on mouse fat for immunofluorescence. I froze mesenteric and epididymal fat at necropsy in isopentane. I am having a hard time getting the tissue to cut-I'm getting the hole in the middle of the OCT block. I have changed the temp in the cryostat to range from -16 to -35 and inbetween to see if colder would help. Not much luck. Does anyone know if I can defrost and fix the tissue and will it improve the cutting or will that cause it to loose any FITC. Thanks for any suggestions. Pam __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From vbs_apps <@t> yahoo.com Mon Mar 14 23:01:52 2005 From: vbs_apps <@t> yahoo.com (JJS) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] IHC stainer In-Reply-To: 6667 Message-ID: <20050315050152.43630.qmail@web50901.mail.yahoo.com> May I suggest that you also take a look at Vision BioSystems and their Bond systems; Bond X and Bond maX. The Bond maX is a fully automated IHC stainer that will also automate ISH. http://www.vision-bio.com Best regards "Rosalia, Anthony [IIG]" wrote: I am trying to get feedback on the current options for IHC stainers. I am familiar with Ventana, Biogenex, and Dako products on paper but would love to get some feedback on their clinical performance to aid in decision making process. Thanks in advance. Anthony Rosalia (212)643-5884 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Mail - You care about security. So do we. From Kemlo.Rogerson <@t> elht.nhs.uk Tue Mar 15 02:20:25 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Advanced Biomedical Scientists[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F091@bhrv-nt-11.bhrv.nwest.nhs.uk> I have recently become involved in a project whose remit is to look at advancing the skills of the Biomedical Scientist in Cellular Pathology. I am completing a critical path process for work passing through a Histology Department to determine bottlenecks and those processes presently carried out by Medical Staff that could be devolved to BMS's; obviously Biomedical Scientists can assist their medical colleagues in a number of areas such as in the 'cut up', reading of ICC and the triage of 'special procedures'. I would be grateful for colleagues' thoughts as to how we may develop Biomedical Scientists to enhance their roles and what these roles exactly are. I am anxious that this is a constructive exercise that will result in the equal delivery of the service nationally and will enhance Biomedical Scientists jobs without stepping on anyone's toes. From abright <@t> brightinstruments.com Tue Mar 15 03:30:13 2005 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Cutting frozen fat Message-ID: Pam, You should have no problem sectioning mesenteric and epididymal fat at a specimen temperature of -30?C, but if your knife and anti-roll plate are at a warmer temperature than the specimen this would explain the results you report. Please come back to me if you require further assistance. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: pam plumlee [mailto:paw555@yahoo.com] Sent: 15 March 2005 03:13 To: HistoNet Server Subject: [Histonet] Cutting frozen fat Hi all: I have a project that the researcher wants frozen sections on mouse fat for immunofluorescence. I froze mesenteric and epididymal fat at necropsy in isopentane. I am having a hard time getting the tissue to cut-I'm getting the hole in the middle of the OCT block. I have changed the temp in the cryostat to range from -16 to -35 and inbetween to see if colder would help. Not much luck. Does anyone know if I can defrost and fix the tissue and will it improve the cutting or will that cause it to loose any FITC. Thanks for any suggestions. Pam __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Inga.Hansson <@t> neuro.uu.se Tue Mar 15 03:55:10 2005 From: Inga.Hansson <@t> neuro.uu.se (Inga Hansson) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] pJNK Message-ID: <1fa94fee9ee21fc9fb3134940f2bb35e@neuro.uu.se> Hi everyone, Has anyone successfully used this antbody on mouse FFPE-sections? It?s supposed to work but I don?t see any signal at all ! I tried diluting the antibody 1:100 and HIER for 10 minutes in citrate! Thanks in advance! Inga Inga Hansson Dept.neuroscience, div. neurobiology P.O. Box 587 Biomedical Centre S-751 23 Uppsala Sweden From Andrew.MacDuff <@t> ed.ac.uk Tue Mar 15 06:06:27 2005 From: Andrew.MacDuff <@t> ed.ac.uk (Andrew MacDuff) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Calculating antibody concentrations Message-ID: <001a01c52957$6abe5800$b9b2d781@universi8lynpq> Dear All I'm trying to find an easy way of calculating the required volumes of 2 stock antibodies to add to a fixed volume of cell suspension so that I can analyse the cells by flow cytometry at the correct Ab dilution for each Ab (ie to allow for the volume of the other Ab added to the sample so that each Ab is at its optimised titer). Can you tell me the maths needed? My poor head hurts and it has been a long time since I did anything more than use my fingers (and occasionally toes!). Many thanks Andrew From Christof.Krug <@t> t-online.de Tue Mar 15 08:05:34 2005 From: Christof.Krug <@t> t-online.de (Christof Krug) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] AW: Automated Image analysis In-Reply-To: <1DBCPz-26Iytk0@fwd18.sul.t-online.de> Message-ID: <1DBCfr-2Cd6dk0@fwd28.sul.t-online.de> Hi Yan I have developed an automatic image analysis software (www.cvistec.com ) for complex biomedical images. It is best suited for high throughput, high content image analysis and tissue microarrays. Customized measurement solutions for your specific images can be add. If you are interested, please get in touch with me. Kind regards Chistof Krug Informatikb?ro Christof Krug Dominikstr.21 D-81929 M?nchen Phone +49 89 99341972 Mobil +49 170 5836734 Fax +49 89 99341974 Email info@cvistec.com WWW www.cvistec.com From sluhisto <@t> yahoo.com Tue Mar 15 09:13:38 2005 From: sluhisto <@t> yahoo.com (Histology SLU) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] ASR disclaimer question Message-ID: <20050315151338.95726.qmail@web51003.mail.yahoo.com> Hello All: I have information from the CAP website from 10/2003 that states: 'The FDA very clearly distinguishes ASR's from immunohistochemistry (IHC) reagents (final rule effective Aug. 17 1998). The FDA defines IHC's as "in vitro diagnostic devices....intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASR's, IHC's are labeled by manufacturers with directions for use and performance indications.' I have checked with all of my vendors and all actually label their antibodies as IVD, research, or ASR. Those companies that have ASR antibodies available always use the letters ASR in the catalog number (at least all of the ones I have contacted). Using all of this information, our lab does not use ANY ASR's, so in you all's professional opinion, must we include the disclaimer? For those of you that have been inspected lately, what specifically are the inspector's comments about this topic? As always, it will be great to hear all of your feedback. Thanks, in advance. Susan __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From juan.gutierrez <@t> christushealth.org Tue Mar 15 09:25:04 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] ASR disclaimer question Message-ID: We only include the disclaimer on cases that we actually use antibodies labeled as ASR. Our pathologists are very picky about the way their reports look, so if we don't use them we don't disclaim. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology SLU Sent: Tuesday, March 15, 2005 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASR disclaimer question Hello All: I have information from the CAP website from 10/2003 that states: 'The FDA very clearly distinguishes ASR's from immunohistochemistry (IHC) reagents (final rule effective Aug. 17 1998). The FDA defines IHC's as "in vitro diagnostic devices....intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASR's, IHC's are labeled by manufacturers with directions for use and performance indications.' I have checked with all of my vendors and all actually label their antibodies as IVD, research, or ASR. Those companies that have ASR antibodies available always use the letters ASR in the catalog number (at least all of the ones I have contacted). Using all of this information, our lab does not use ANY ASR's, so in you all's professional opinion, must we include the disclaimer? For those of you that have been inspected lately, what specifically are the inspector's comments about this topic? As always, it will be great to hear all of your feedback. Thanks, in advance. Susan __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pzeitlow <@t> bbpllab.com Tue Mar 15 09:29:37 2005 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] ASR disclaimer question Message-ID: <813FB33DA405334F947F8BFC6EBD0B2AE69A@bbplsrv1.bbpl> Interestingly, I just got inspected yesterday and had a lengthy discussion with the inspector on ASR disclaimers. My strong opinion is that you do not need to use the disclaimer unless you are using antibodies or reagents that would be considered "home brew" (i.e. Detection systems not purchased ready to use, antibodies with no instructions for use, etc.). All of the reagents and antibodies that we use (regardless of manufacturer labeling) come with directions for use and performance indications, therefore we do not use the disclaimer. Naturally we validate every antibody and reagent before placing any protocol in use. There is a lengthy commentary in the newest checklist that I think clearly supports our decision not to use the disclaimer. The quote you included says it all. I produced a copy of the final rules with that highlighted for the inspector. I did not get sited nor was it recommended that I add the disclaimer. I look forward to the many opinions I will read on the Histonet. Pat -----Original Message----- From: Histology SLU [mailto:sluhisto@yahoo.com] Sent: Tuesday, March 15, 2005 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASR disclaimer question Hello All: I have information from the CAP website from 10/2003 that states: 'The FDA very clearly distinguishes ASR's from immunohistochemistry (IHC) reagents (final rule effective Aug. 17 1998). The FDA defines IHC's as "in vitro diagnostic devices....intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASR's, IHC's are labeled by manufacturers with directions for use and performance indications.' I have checked with all of my vendors and all actually label their antibodies as IVD, research, or ASR. Those companies that have ASR antibodies available always use the letters ASR in the catalog number (at least all of the ones I have contacted). Using all of this information, our lab does not use ANY ASR's, so in you all's professional opinion, must we include the disclaimer? For those of you that have been inspected lately, what specifically are the inspector's comments about this topic? As always, it will be great to hear all of your feedback. Thanks, in advance. Susan __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Tue Mar 15 09:55:03 2005 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] H&E Staining Message-ID: I'll second that. I currently have the DRS 2000 and have used a 600. Both worked great. I have no negative comments on either. Fred >>> Patti Loykasek 03/14/05 06:51PM >>> We are using a Sakura stainer. We do H&E's, deparaffinize/peroxidase block our immunos, & counterstain our immunos on it. I like the fact that it can run different programs at the same time. It has a good throughput, too. Please contact me if you need more specifics.> Patti Loykasek PhenoPath Laboratories Seattle, WA As of today H&E staining is a manual process in our lab. Would love to hear > any feedback on automating this process. I run one busy practice, and one I > would consider pretty small. > > Thanks, > Anthony Rosalia > (212)643-5884 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Mar 15 10:03:41 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Cutting frozen fat In-Reply-To: <20050315031249.44880.qmail@web11603.mail.yahoo.com> References: <20050315031249.44880.qmail@web11603.mail.yahoo.com> Message-ID: <6.0.0.22.1.20050315085546.01b42878@gemini.msu.montana.edu> -35 may NOT be cold enough for cryosectioning fat. That is probably why there are holes where fat comes out of OCT as the fat is just too soft, not in a truly solid state. Try cooling the block and knife with dry ice to lower the temperature further and see if that helps. Be sure to not give yourself frostbite. We were able to cut mink skin which contains almost pure mink oil for the purpose of seeing hair follicles, the lipid was basically liquid using Intrumedics tape transfer. The fatty parts of skin really did not stay on the tape very well but hair follicles were glued down nicely. I know one hospital laboratory who used this instrumentation to cut frozen sections of fatty human breast with success. Before thawing and trying to fix the fat, go to colder temperature instead. At 08:12 PM 3/14/2005, you wrote: >Hi all: I have a project that the researcher wants >frozen sections on mouse fat for immunofluorescence. >I froze mesenteric and epididymal fat at necropsy in >isopentane. I am having a hard time getting the >tissue to cut-I'm getting the hole in the middle of >the OCT block. I have changed the temp in the >cryostat to range from -16 to -35 and inbetween to see >if colder would help. Not much luck. Does anyone know >if I can defrost and fix the tissue and will it >improve the cutting or will that cause it to loose any >FITC. Thanks for any suggestions. Pam > > > >__________________________________ >Do you Yahoo!? >Yahoo! Small Business - Try our new resources site! >http://smallbusiness.yahoo.com/resources/ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDUE <@t> PARTNERS.ORG Tue Mar 15 10:06:55 2005 From: BDUE <@t> PARTNERS.ORG (Due, Brice) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Cutting frozen fat Message-ID: <59C772E2D8EDF345AE1601F5B60B3CB5027950@PHSXMB7.partners.org> Hello Pam, I have successfully cut 10um sections of human muscle bxs with end-stage myopathies where the muscle had been 99% replaced with fat. The trick as Alan Bright pointed out is to make sure nothing that touches the sections is warmer than the block. Dry ice is my trick. I use pieces of dry ice to chill the block, stage, blade, and roll plates as I'm cutting. I use pelletized dry ice. If I am careful I can place several pieces on the stage/blade holder without them getting in the way of the roll plate. I leave one piece hanging over the blade edge. Between sections I rub the roll plate with a piece of dry ice. My chuck holder allows me to place a piece of dry ice on top of the chuck without getting in the way of the blade. So basically I put dry ice everywhere and crank the chamber down as far as it will go. I usually cut 10um sections with a roll plate. The end-stage myopathy I mentioned above had to be cut at 14um even with dry ice. The OCT in thinner sections may not hold together well at dry ice temps. So you may have to experiment with timings. How long you chill the block face or how long you wait after you remove the dry ice from the specimen before you start sectioning. Sometimes I find that I get best results by chilling the block face and then pressing my thumb to the block between sections. Playing with dry ice gives you access to temps from -79C up to the chamber temp. One further dry-ice trick: At if you have a small cup of dry ice in the cryostat you can chill your foreceps. At such low temps, you can actually handle OCT sections with forceps. The sections do not stick to the forceps until your hand warms them up. I have used chilled foreceps to pick and place sections on slides, and I have used the forceps in place of a brush when cutting with the "brush" technique. Good luck! -brice Brigham & Women's Neuropathology Boston, MA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of pam plumlee Sent: Monday, March 14, 2005 10:13 PM To: HistoNet Server Subject: [Histonet] Cutting frozen fat Hi all: I have a project that the researcher wants frozen sections on mouse fat for immunofluorescence. I froze mesenteric and epididymal fat at necropsy in isopentane. I am having a hard time getting the tissue to cut-I'm getting the hole in the middle of the OCT block. I have changed the temp in the cryostat to range from -16 to -35 and inbetween to see if colder would help. Not much luck. Does anyone know if I can defrost and fix the tissue and will it improve the cutting or will that cause it to loose any FITC. Thanks for any suggestions. Pam __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sluhisto <@t> yahoo.com Tue Mar 15 11:48:50 2005 From: sluhisto <@t> yahoo.com (Histology SLU) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] ASR disclaimer question In-Reply-To: 6667 Message-ID: <20050315174850.5846.qmail@web51004.mail.yahoo.com> Tim: I did not mean to imply that all companies use the ASR, IVD, RUO in their catalog numbers. What I meant was that the companies that I spoke with, some you mention by the way, told me that they do list ASR in the catalog number, if the reagent is such; specifically meaning that if they had a catalog number without ASR in it, then it was to be considered IVD or RUO. Hope this clears it up. Susan "Morken, Tim - Labvision" wrote: Susan, Our company does not use "ASR", "IVD" or "RUO" in the catalog number, nor do any of the other top antibody vendors (DAKO, Novocastra, Zymed, BioGenex, BioCare), so rather than depend on catalog numbers, look at the datasheet that comes with the antibody. The datasheet should clearly state either ASR, IVD or RUO use. Also, the label on the antibody vial should clearly state "in vitro diagnostic device" if it is an IVD, ASR or RUO. If it does not, then contact the vendor to clarify. Tim Morken Product Development Lab Vision - Neomarkers 47790 Westinghouse Dr. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 email: tpmorken@labvision.com web: www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology SLU Sent: Tuesday, March 15, 2005 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASR disclaimer question Hello All: I have information from the CAP website from 10/2003 that states: 'The FDA very clearly distinguishes ASR's from immunohistochemistry (IHC) reagents (final rule effective Aug. 17 1998). The FDA defines IHC's as "in vitro diagnostic devices....intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASR's, IHC's are labeled by manufacturers with directions for use and performance indications.' I have checked with all of my vendors and all actually label their antibodies as IVD, research, or ASR. Those companies that have ASR antibodies available always use the letters ASR in the catalog number (at least all of the ones I have contacted). Using all of this information, our lab does not use ANY ASR's, so in you all's professional opinion, must we include the disclaimer? For those of you that have been inspected lately, what specifically are the inspector's comments about this topic? As always, it will be great to hear all of your feedback. Thanks, in advance. Susan __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From jlinda <@t> ces.clemson.edu Tue Mar 15 12:46:10 2005 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Re: Cutting frozen fat Message-ID: <5.2.1.1.2.20050315133430.00aac480@mailhost.ces.clemson.edu> Pam, Your message read: "Hi all: I have a project that the researcher wants frozen sections on mouse fat for immunofluorescence. I froze mesenteric and epididymal fat at necropsy in isopentane. I am having a hard time getting the tissue to cut-I'm getting the hole in the middle of the OCT block. I have changed the temp in the cryostat to range from -16 to -35 and inbetween to see if colder would help. Not much luck. Does anyone know if I can defrost and fix the tissue and will it improve the cutting or will that cause it to loose any FITC. Thanks for any suggestions. Pam" First of all...How did you store the specimens until cryosectioning? If you used a -80C freezer, I have found that it is useful to allow 1/2 day for acclimating to the -30C cryostat temp. Also, for holes in the sample, I use a wooden tongue depressor which has a drop of fresh freezing compound (OCT, etc) placed on one end and, using a "spackling" technique, I quickly fill the hole with fresh freezing compound and allow it to refreeze. Remember to back off on your knife setting as this will increase the height of the sample. Happy cutting! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From traphp <@t> parknicollet.com Tue Mar 15 14:15:55 2005 From: traphp <@t> parknicollet.com (Traphagan, Patricia) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] coverslippers Message-ID: We are having major air bubble problems after we have coversliped our slides using the Leica coverslipper. We use Cytoseal and Richard Allen coverslippers. We set the dials at 2.5 volume and 1 mountant type. Patty Traphagan Histology Technical Specialist Methodist Hospital Laboratory 952-993-2823 or 952-993-5304 PRIVACY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain business confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If this e-mail was not intended for you, please notify the sender by reply e-mail that you received this in error. Destroy all copies of the original message and attachments. From jengirl1014 <@t> yahoo.com Tue Mar 15 14:22:50 2005 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] processing Message-ID: <20050315202250.95682.qmail@web60605.mail.yahoo.com> I have some tissue that I processed and it came out a little bit drier than I thought it would. Is it possible to take it backwards through the processing and rehydrate it and then process it again, BUT at shorter times to ensure that the tissue would be processed properly the second time? Thanks in advance! Happy St. Patrick's Day! Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gcallis <@t> montana.edu Tue Mar 15 14:53:25 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] processing In-Reply-To: <20050315202250.95682.qmail@web60605.mail.yahoo.com> References: <20050315202250.95682.qmail@web60605.mail.yahoo.com> Message-ID: <6.0.0.22.1.20050315134812.01b64470@gemini.msu.montana.edu> Jennifer, I doubt this would help but rather make it worse as you have already removed both the free water in tissue (what you want to do) but if you take it backwards through alcohols again, you are going to further remove more of the bound water (that you want to retain) on proteins. The best thing to do is try soaking tissues after facing the block, with warm water then cool the block and cut. Even if you rehydrated it and then reprocessed, any water you put into tissue spaces is probably going to be removed again plus reexposure to heat of paraffin is going to compound the issue, cooked twice over - so to speak. Best is rethink the processing schedule for your particular tissue and use shorter processing times in future. At 01:22 PM 3/15/2005, you wrote: >I have some tissue that I processed and it came out a little bit drier >than I thought it would. Is it possible to take it backwards through the >processing and rehydrate it and then process it again, BUT at shorter >times to ensure that the tissue would be processed properly the second >time? Thanks in advance! > >Happy St. Patrick's Day! > > >Jennifer K. Sipes, RALAT >Sr. Laboratory Technician >Johns Hopkins University >Ross 929 >720 Rutland Avenue >Baltimore, MD 21205 >phone: 410-614-0131 >cell: 443-413-0853 >e-mail: jengirl1014@yahoo.com > > > > > > > > > > >__________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From AnthonyH <@t> chw.edu.au Tue Mar 15 15:01:39 2005 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] AW: Automated Image analysis Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3741269E1E5@simba.kids> This seems to be a blatant addvertisement! Christof this is not allowed on histonet Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Christof Krug [mailto:Christof.Krug@t-online.de] Sent: Wednesday, 16 March 2005 1:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AW: Automated Image analysis Hi Yan I have developed an automatic image analysis software (www.cvistec.com ) for complex biomedical images. It is best suited for high throughput, high content image analysis and tissue microarrays. Customized measurement solutions for your specific images can be add. If you are interested, please get in touch with me. Kind regards Chistof Krug Informatikb?ro Christof Krug Dominikstr.21 D-81929 M?nchen Phone +49 89 99341972 Mobil +49 170 5836734 Fax +49 89 99341974 Email info@cvistec.com WWW www.cvistec.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From tpmorken <@t> labvision.com Tue Mar 15 15:15:48 2005 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] ASR disclaimer question Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CAF6@usca0082k08.labvision.apogent.com> Pat, Disclaimer: I am part of a company that is FDA registerd to sell ASR and IVD antibodies, and RUO antibodies and IVD detection systems. The part of the CAP checklist you mention (reproduced below) is not new as far as ASR's are concerned, but is new for RUO's. ASR's were introduced to allow vendors to sell, and labs to use, antibodies that did not meet the "exempt" criteria of the Class I IVD IHC reagents. Exempt antibodies are those that are used in conjuction with other tests - antibody or otherwise - to reach a conclusion. Most of the antibodies being sold as ASR's today are those that are meant to be stand-alone predictive or prognostic tests (ER, PR, etc) and so require either FDA premarket approval - requiring validation testing by the manufacturer, or are Class III infectious agent antibodies that require extensive proof they detect what they are supposed to detect. ASR's must be sold to only CLIA certified clinical diagnostic labs and the vendor cannot include any information about how to use the antibody - the lab is supposed to validate the antibody in-house. However, ASR's are sold only by FDA-registered vendors, so are under GMP guidelines (good manufacturing practices) and are inspected by the FDA, so you do have some assurance that the antibodies are at least working right (the vendor must show the antibody labels the target molecule by some kind of test - western blost, elisa, IHC). If all your antibodies come with use instructions, then you are getting either IVD or RUO antibodies. If you use RUO antibodies, then,according to this new checklist, you must make a reasonable effort to find a supplier that is FDA registered (most RUO-only suppliers are not FDA registered and are not supposed to sell to clinical labs for clinical use applications). Apparently the CAP has recognized that some antibodies are not available through FDA registered vendors, so have allowed labs this as a way out of that problem. That is going to open a whole new can of worms because now labs are going to use antibodies that have been manufactured under no requirements at all - FDA registerd labs must follow GMP (good manufacturing practices) and under go FDA inspections. RUO-only vendors have none of these requirements. I would say that getting ASR's is a better bet than getting RUO's in this case. An RUO vendor does not have to test the antibody at all. What is really interesting is that CAP apparently makes no requirement to put in any disclaimer about the use of RUO antibodies. I can't find anything about that in the general lab checklist either. It seems, then, that a lab can use RUO's like IVD's now. So, maybe you are in the situation that you don't use any ASR's but do use IVD and RUO antibodies. From how I read the current CAP comments, you are right that you don't need any disclaimer. It's now become a really strange situation! If someone sees it differently I'd like to hear any comments. As far as "home brew" terminology is concerned, the FDA has defined "home brew" as any test that is not a complete, self-contained, FDA-approved kit. The primary reason they developed this whole IVD/ASR/RUO system is so that labs CAN make home brew tests. With this system a lab can mix and match antibodies and detection systems to their hearts content. The only part the FDA is concerned about is the analytic part of the test - the specific probe that detects the specific target. All the detection systems are termed "general laboratory reagents" and are not considered under this system (though detection kits can be termed IVD if they are produced by a FDA registered vendor). In fact, it is only if you BUY the reagent that FDA applies these rules: FDA has said that if you make the antibody in house, it is not considered an ASR, IVD or RUO - and you don't need any disclaimers (assuming you a CLIA certified lab an the lab still needs to document the validation procedure). Tim Morken Lab Vision - Neomarkers www.labvision.com ASR/IVD/RUO commentary **REVISED** 12/01/2003 ANP.12425 Phase II N/A YES NO If patient testing is performed using Class I analyte-specific reagents (ASR's) obtained or purchased from an outside vendor, does the patient report include the disclaimer required by federal regulations? NOTE: ASR's are antibodies, both polyclonal and monoclonal, specific receptor proteins, ligands, nucleic acid sequences, and similar reagents which, through specific binding or chemical reaction with substances in a specimen, are intended for use in a diagnostic application for identification and quantification of an individual chemical substance or ligand in biological specimens. By definition, an ASR is the active ingredient of an in-house-developed ("home brew") test system. ASR's may be obtained from outside vendors or synthesized in-house. ASR's from outside vendors are supplied individually. They are not bundled with other materials in kit form, and the accompanying product literature does not include any claims with respect to use or performance of the reagent. Class I ASR's in use in the anatomic pathology laboratory include some antibodies for immunohistochemistry and nucleic acid probes for FISH and ISH. Class I ASR's are not subject to preclearance by the U.S. Food and Drug Administration or to special controls by FDA. Thus, if the laboratory performs patient testing using Class I ASR's obtained or purchased from an outside vendor, federal regulations require that the following disclaimer accompany the test result on the patient report: "This test was developed and its performance characteristics determined by (laboratory name). It has not been cleared or approved by the U.S. Food and Drug Administration." The CAP recommends additional language, such as "The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing." The above disclaimer is not required when using reagents that are sold in kit form with other materials and/or an instrument, and/or with instructions for use, and/or when labeled by the manufacturer as Class I for in vitro diagnostic use (IVD), Class II IVD, or Class III IVD. Most antibodies used in immunohistochemistry are labeled "for in vitro diagnostic use" and thus do NOT require the disclaimer. Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew tests only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director. The laboratory must establish or verify the performance characteristics of tests using Class I ASR's and RUO's in accordance with the Method Performance Specifications section of the Laboratory General checklist. The laboratory may put an ASR disclaimer on the pathology report for all immunostains, FISH and ISH studies collectively used in a particular case. Separately tracking each reagent used for a case and selectively applying the disclaimer to only the class I ASR's is unnecessary. COMMENTARY: N/A REFERENCES: 1) Department of Health and Human Services, Food and Drug Administration. Medical devices; classification/reclassification; restricted devices; analyte specific reagents. Final rule. Fed Register. 1997(Nov 21);62243-45 [21CFR809, 21CFR864]; 2) Caldwell CW. Analyte-specific reagents in the flow cytometry laboratory. Arch Pathol Lab Med. 1998;122:861-864; 3) Graziano. Disclaimer now needed for analyte-specific reagents. Northfield, IL: College of American Pathologists CAP Today. 1998;12(11):5-11; 4) Analyte Specific Reagents; Small Entity Compliance Guidance. http://www.fda.gov/cdrh/oivd/guidance/1205.html, February 26, 2003; 5) Shapiro JD and Prebula RJ. FDA's Regulation of Analyte-Specific Reagents. Medical Devicelink, February 2003. http://www.devicelink.com/mddi/archive/03/02/018.html; 6) U.S. Department of Health and Human Services, Food and Drug Administration. www.fda.gov/cdrh/ode/immuno.html; 7) U.S. Department of Health and Human Services, Food and Drug Administration. http://www.fda.gov/cdrh/oivd/index.html. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Tuesday, March 15, 2005 7:30 AM To: Histology SLU; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASR disclaimer question Interestingly, I just got inspected yesterday and had a lengthy discussion with the inspector on ASR disclaimers. My strong opinion is that you do not need to use the disclaimer unless you are using antibodies or reagents that would be considered "home brew" (i.e. Detection systems not purchased ready to use, antibodies with no instructions for use, etc.). All of the reagents and antibodies that we use (regardless of manufacturer labeling) come with directions for use and performance indications, therefore we do not use the disclaimer. Naturally we validate every antibody and reagent before placing any protocol in use. There is a lengthy commentary in the newest checklist that I think clearly supports our decision not to use the disclaimer. The quote you included says it all. I produced a copy of the final rules with that highlighted for the inspector. I did not get sited nor was it recommended that I add the disclaimer. I look forward to the many opinions I will read on the Histonet. Pat -----Original Message----- From: Histology SLU [mailto:sluhisto@yahoo.com] Sent: Tuesday, March 15, 2005 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASR disclaimer question Hello All: I have information from the CAP website from 10/2003 that states: 'The FDA very clearly distinguishes ASR's from immunohistochemistry (IHC) reagents (final rule effective Aug. 17 1998). The FDA defines IHC's as "in vitro diagnostic devices....intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASR's, IHC's are labeled by manufacturers with directions for use and performance indications.' I have checked with all of my vendors and all actually label their antibodies as IVD, research, or ASR. Those companies that have ASR antibodies available always use the letters ASR in the catalog number (at least all of the ones I have contacted). Using all of this information, our lab does not use ANY ASR's, so in you all's professional opinion, must we include the disclaimer? For those of you that have been inspected lately, what specifically are the inspector's comments about this topic? As always, it will be great to hear all of your feedback. Thanks, in advance. Susan __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.babri <@t> uq.edu.au Tue Mar 15 16:06:33 2005 From: a.babri <@t> uq.edu.au (Awais BABRI) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Pentachrome staining Message-ID: <42375C69.4040408@uq.edu.au> I am looking for the pentachrome staining technique which appeared in Iranian Journal of Medical Science, an article by Haghighi S. Does any one have the protocol for his technique. Awaiting. Saleem. From dpahisto <@t> yahoo.com Tue Mar 15 17:12:00 2005 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Starting ER/PR Message-ID: <20050315231200.64347.qmail@web41309.mail.yahoo.com> Our doctors are considering starting ER/PR studies in our lab. I currently stain all immunos by hand, but they said they would buy a stainer if we add ER/PR to our protocols. Where do I start to obtain information on staining procedures, requirements, etc... thanks, Cindy DuBois Delta Pathology Assoc. --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From immrstambo <@t> hotmail.com Tue Mar 15 19:37:53 2005 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] I use the Fisher Microprobe Staining system In-Reply-To: <4235E0B5.6010606@bms.com> Message-ID: I love the Fisher Microprobe System, it is simple and easy for a small lab to get good consistent staining. It's reasonably priced too, and you save money in reagents. I hope this helps- Good Luck! Christine Tambasco HT (ASCP) St. Mary's Hospital Amsterdam, New York 12070 ph-518-841-7287 >From: Kelly D Mcqueeney >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Anyone use the Fisher Microprobe Staining system? >Date: Mon, 14 Mar 2005 14:06:29 -0500 > >Hi Histonetters, >Has anyone used the Fisher Microprobe Staining system? Do you have >any problems? We are interested in a very simple staining system for >only one 60-minute incubation in hot ligand. I have used the Thermo >Sequenza but we are having technical issues, plus loading the slides >is a pain. We do not work with more than 20 slides at a time (and >using radioligand, clean-up is an issue). I would appreciate no >response from reps concerning complex, automated systems, we have >already determined they are not right for us. > >Thanks, >Kelly > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 16 01:51:13 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] processing[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0A0@bhrv-nt-11.bhrv.nwest.nhs.uk> Wasn't there a procedure in Lillie's book about regenerating 'overcooked' tissue? I fancy it involved using glycerol or something like that; I'll have a scout around but I'm sure I used it when I was a pup. -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 15 March 2005 20:53 To: Jennifer Sipes; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] processing[Scanned] Jennifer, I doubt this would help but rather make it worse as you have already removed both the free water in tissue (what you want to do) but if you take it backwards through alcohols again, you are going to further remove more of the bound water (that you want to retain) on proteins. The best thing to do is try soaking tissues after facing the block, with warm water then cool the block and cut. Even if you rehydrated it and then reprocessed, any water you put into tissue spaces is probably going to be removed again plus reexposure to heat of paraffin is going to compound the issue, cooked twice over - so to speak. Best is rethink the processing schedule for your particular tissue and use shorter processing times in future. At 01:22 PM 3/15/2005, you wrote: >I have some tissue that I processed and it came out a little bit drier >than I thought it would. Is it possible to take it backwards through the >processing and rehydrate it and then process it again, BUT at shorter >times to ensure that the tissue would be processed properly the second >time? Thanks in advance! > >Happy St. Patrick's Day! > > >Jennifer K. Sipes, RALAT >Sr. Laboratory Technician >Johns Hopkins University >Ross 929 >720 Rutland Avenue >Baltimore, MD 21205 >phone: 410-614-0131 >cell: 443-413-0853 >e-mail: jengirl1014@yahoo.com > > > > > > > > > > >__________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 16 01:55:03 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] coverslippers[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0A1@bhrv-nt-11.bhrv.nwest.nhs.uk> Have you de-gassed the mountant, sometimes it can be a bit like TIZER. Air leaks also cause bubbles, too. -----Original Message----- From: Traphagan, Patricia [mailto:traphp@parknicollet.com] Sent: 15 March 2005 20:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslippers[Scanned] We are having major air bubble problems after we have coversliped our slides using the Leica coverslipper. We use Cytoseal and Richard Allen coverslippers. We set the dials at 2.5 volume and 1 mountant type. Patty Traphagan Histology Technical Specialist Methodist Hospital Laboratory 952-993-2823 or 952-993-5304 PRIVACY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain business confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If this e-mail was not intended for you, please notify the sender by reply e-mail that you received this in error. Destroy all copies of the original message and attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Wed Mar 16 01:59:27 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Cutting frozen fat Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114A6@mius2.medlan.cam.ac.uk> One thought on this subject: If you are setting up a lab with the knowledge that you will be cryosectioning fat, consider buying a cryostat with twin compressors, these will maintain much lower temperatures than single compressor versions and will attain low temperature quicker, with the added advantage that if one compressor fails, your cryostat will still work normally. Just a thought for anyone considering a purchase. The twin compressor cryostat I had came from Bright Instruments. Regards Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Due, Brice [mailto:BDUE@PARTNERS.ORG] Sent: Tuesday, March 15, 2005 4:07 PM To: pam plumlee; HistoNet Server Subject: RE: [Histonet] Cutting frozen fat Hello Pam, I have successfully cut 10um sections of human muscle bxs with end-stage myopathies where the muscle had been 99% replaced with fat. The trick as Alan Bright pointed out is to make sure nothing that touches the sections is warmer than the block. Dry ice is my trick. I use pieces of dry ice to chill the block, stage, blade, and roll plates as I'm cutting. I use pelletized dry ice. If I am careful I can place several pieces on the stage/blade holder without them getting in the way of the roll plate. I leave one piece hanging over the blade edge. Between sections I rub the roll plate with a piece of dry ice. My chuck holder allows me to place a piece of dry ice on top of the chuck without getting in the way of the blade. So basically I put dry ice everywhere and crank the chamber down as far as it will go. I usually cut 10um sections with a roll plate. The end-stage myopathy I mentioned above had to be cut at 14um even with dry ice. The OCT in thinner sections may not hold together well at dry ice temps. So you may have to experiment with timings. How long you chill the block face or how long you wait after you remove the dry ice from the specimen before you start sectioning. Sometimes I find that I get best results by chilling the block face and then pressing my thumb to the block between sections. Playing with dry ice gives you access to temps from -79C up to the chamber temp. One further dry-ice trick: At if you have a small cup of dry ice in the cryostat you can chill your foreceps. At such low temps, you can actually handle OCT sections with forceps. The sections do not stick to the forceps until your hand warms them up. I have used chilled foreceps to pick and place sections on slides, and I have used the forceps in place of a brush when cutting with the "brush" technique. Good luck! -brice Brigham & Women's Neuropathology Boston, MA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of pam plumlee Sent: Monday, March 14, 2005 10:13 PM To: HistoNet Server Subject: [Histonet] Cutting frozen fat Hi all: I have a project that the researcher wants frozen sections on mouse fat for immunofluorescence. I froze mesenteric and epididymal fat at necropsy in isopentane. I am having a hard time getting the tissue to cut-I'm getting the hole in the middle of the OCT block. I have changed the temp in the cryostat to range from -16 to -35 and inbetween to see if colder would help. Not much luck. Does anyone know if I can defrost and fix the tissue and will it improve the cutting or will that cause it to loose any FITC. Thanks for any suggestions. Pam __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rentonlf <@t> bru.wits.ac.za Wed Mar 16 01:53:43 2005 From: rentonlf <@t> bru.wits.ac.za (renton louise mrs) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Off Topic: Automated Image analysis Message-ID: <1110959623.8e6fb4dcrentonlf@bru.wits.ac.za> Actually I see no difference between this and Instrumedics' standard reply to any cutting or cryosectioning problem...perhaps we should put a ban on all punting of commercial wares regards -----Original Message----- From: Tony Henwood To: "'Christof Krug'" Date: Wed, 16 Mar 2005 08:01:39 +1100 Subject: RE: [Histonet] AW: Automated Image analysis This seems to be a blatant addvertisement! Christof this is not allowed on histonet Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Christof Krug [mailto:Christof.Krug@t-online.de] Sent: Wednesday, 16 March 2005 1:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AW: Automated Image analysis Hi Yan I have developed an automatic image analysis software (www.cvistec.com ) for complex biomedical images. It is best suited for high throughput, high content image analysis and tissue microarrays. Customized measurement solutions for your specific images can be add. If you are interested, please get in touch with me. Kind regards Chistof Krug Informatikb?ro Christof Krug Dominikstr.21 D-81929 M?nchen Phone +49 89 99341972 Mobil +49 170 5836734 Fax +49 89 99341974 Email info@cvistec.com WWW www.cvistec.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? From marco.prunotto <@t> medecine.unige.ch Wed Mar 16 03:29:47 2005 From: marco.prunotto <@t> medecine.unige.ch (Marco Prunotto) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] history of staining techs Message-ID: <4237FC8B.3040208@medecine.unige.ch> Dear All, I'm post-doc in prof. Gabbiani Lab in the Geneva Pathology Dept., Medicine Faculty. I'm also interested in history of Science. Have you got any idea of books or articles on history of staining techs and histological preparation in general? thanks a lot Marco Prunotto From darkdaym <@t> mindspring.com Wed Mar 16 05:16:02 2005 From: darkdaym <@t> mindspring.com (Mark Ray) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Off Topic: Automated Image analysis In-Reply-To: <1110959623.8e6fb4dcrentonlf@bru.wits.ac.za> References: <1110959623.8e6fb4dcrentonlf@bru.wits.ac.za> Message-ID: <42381572.1030806@mindspring.com> Louise and Tony and all the rest, I see this issue as slightly more complex. Intsrumedics' postings are generally a reply to someone's posted request for help. Such things are supposed to be OK on Histonet. I think you would agree, Tony, that vendor response to specific help requests is not necessarily a bad thing. Lab workers don't always have knowledge of every specialized product. Christof's posting doesn't fall into this category, however. It is an _unsolicited announcement_ of a new, apparently unique, product. I'm not exactly sure how I feel about this. Maybe Christof is doing a service to people who could use his software and might not otherwise hear about it for a while. This is the only announcement we've seen from him, it's not very long, obtrusive or hard sell. Maybe Histonet should allow these kind of postings if they don't become too annoying or get in the way. Pests have been flamed out of existence in the past and certainly could be in the future. Mark Ray renton louise mrs wrote: >Actually I see no difference between this and Instrumedics' standard reply to any cutting or cryosectioning problem...perhaps we should put a ban on all punting of commercial wares >regards > >-----Original Message----- >From: Tony Henwood >To: "'Christof Krug'" >Date: Wed, 16 Mar 2005 08:01:39 +1100 >Subject: RE: [Histonet] AW: Automated Image analysis > >This seems to be a blatant addvertisement! >Christof this is not allowed on histonet > > >Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) >Laboratory Manager & Senior Scientist >The Children's Hospital at Westmead, >Locked Bag 4001, Westmead, 2145, AUSTRALIA. >Tel: 612 9845 3306 >Fax: 612 9845 3318 > > > > > >-----Original Message----- >From: Christof Krug [mailto:Christof.Krug@t-online.de] >Sent: Wednesday, 16 March 2005 1:06 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] AW: Automated Image analysis > > >Hi Yan > >I have developed an automatic image analysis software (www.cvistec.com > ) for complex biomedical images. It is best suited >for high throughput, high content image analysis and tissue microarrays. >Customized measurement solutions for your specific images can be add. If you >are interested, please get in touch with me. > >Kind regards >Chistof Krug > >Informatikb?ro Christof Krug >Dominikstr.21 >D-81929 M?nchen >Phone +49 89 99341972 >Mobil +49 170 5836734 >Fax +49 89 99341974 >Email info@cvistec.com >WWW www.cvistec.com > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >********************************************************************** >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient, please >delete it and notify the sender. > >Views expressed in this message and any attachments are those >of the individual sender, and are not necessarily the views of The >Children's Hospital at Westmead > >This footnote also confirms that this email message has been >virus scanned and although no computer viruses were detected, >the Childrens Hospital at Westmead accepts no liability for any >consequential damage resulting from email containing computer >viruses. >********************************************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Louise Renton >Bone Research Unit >University of the Witwatersrand >Johannesburg >South Africa >.......so what IS the speed of dark? > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Christof.Krug <@t> t-online.de Wed Mar 16 05:30:03 2005 From: Christof.Krug <@t> t-online.de (Christof Krug) Date: Fri Sep 16 15:24:46 2005 Subject: WG: [Histonet] AW: Automated Image analysis Message-ID: <1DBWiu-0N1hvU0@fwd24.sul.t-online.de> Sorry, forgot to send it to histonet too. Christof -----Urspr?ngliche Nachricht----- Von: Christof Krug [mailto:Christof.Krug@t-online.de] Gesendet: Mittwoch, 16. M?rz 2005 08:43 An: 'Tony Henwood' Betreff: AW: [Histonet] AW: Automated Image analysis Hi Tony I carefully read the rules for histonet: "Vendors and those with commercial interests in histology products are welcome contributors however,we ask that blatant advertisements be avoided at all times. It is fine to refer to product that your company produces if it is pertinent to a topic being discussed on the list. Unsolicited advertisements are poorly tolerated by the members and you will likely receive a number of negative comments if you overstep the boundaries." Since I replied to a question from the archive of a member of histonet, I do not feel this is blatant advertisement, but according to the rules. Regards Christof Informatikb?ro Christof Krug Dominikstr.21 D-81929 M?nchen Phone +49 89 99341972 Mobil +49 170 5836734 Fax +49 89 99341974 Email info@cvistec.com WWW www.cvistec.com -----Urspr?ngliche Nachricht----- Von: Tony Henwood [mailto:AnthonyH@chw.edu.au] Gesendet: Dienstag, 15. M?rz 2005 22:02 An: 'Christof Krug' Cc: 'histonet@lists.utsouthwestern.edu' Betreff: RE: [Histonet] AW: Automated Image analysis This seems to be a blatant addvertisement! Christof this is not allowed on histonet Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Christof Krug [mailto:Christof.Krug@t-online.de] Sent: Wednesday, 16 March 2005 1:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AW: Automated Image analysis Hi Yan I have developed an automatic image analysis software (www.cvistec.com ) for complex biomedical images. It is best suited for high throughput, high content image analysis and tissue microarrays. Customized measurement solutions for your specific images can be add. If you are interested, please get in touch with me. Kind regards Chistof Krug Informatikb?ro Christof Krug Dominikstr.21 D-81929 M?nchen Phone +49 89 99341972 Mobil +49 170 5836734 Fax +49 89 99341974 Email info@cvistec.com WWW www.cvistec.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From JWEEMS <@t> sjha.org Wed Mar 16 05:46:08 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Off Topic: Automated Image analysis Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D204@sjhaexc02.sjha.org> And like Instrumedics, this a reply to a question that was posted. He addressed someone by name. If I had been interested in the same product, I would have appreciated hearing about it. I wasn't so it was no problem to delete. Chill everybody....j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mark Ray Sent: Wed 3/16/2005 6:16 AM To: renton louise mrs Cc: histonet@lists.utsouthwestern.edu; AnthonyH@chw.edu.au Subject: Re: [Histonet] Off Topic: Automated Image analysis Louise and Tony and all the rest, I see this issue as slightly more complex. Intsrumedics' postings are generally a reply to someone's posted request for help. Such things are supposed to be OK on Histonet. I think you would agree, Tony, that vendor response to specific help requests is not necessarily a bad thing. Lab workers don't always have knowledge of every specialized product. Christof's posting doesn't fall into this category, however. It is an _unsolicited announcement_ of a new, apparently unique, product. I'm not exactly sure how I feel about this. Maybe Christof is doing a service to people who could use his software and might not otherwise hear about it for a while. This is the only announcement we've seen from him, it's not very long, obtrusive or hard sell. Maybe Histonet should allow these kind of postings if they don't become too annoying or get in the way. Pests have been flamed out of existence in the past and certainly could be in the future. Mark Ray renton louise mrs wrote: >Actually I see no difference between this and Instrumedics' standard reply to any cutting or cryosectioning problem...perhaps we should put a ban on all punting of commercial wares >regards > >-----Original Message----- >From: Tony Henwood >To: "'Christof Krug'" >Date: Wed, 16 Mar 2005 08:01:39 +1100 >Subject: RE: [Histonet] AW: Automated Image analysis > >This seems to be a blatant addvertisement! >Christof this is not allowed on histonet > > >Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) >Laboratory Manager & Senior Scientist >The Children's Hospital at Westmead, >Locked Bag 4001, Westmead, 2145, AUSTRALIA. >Tel: 612 9845 3306 >Fax: 612 9845 3318 > > > > > >-----Original Message----- >From: Christof Krug [mailto:Christof.Krug@t-online.de] >Sent: Wednesday, 16 March 2005 1:06 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] AW: Automated Image analysis > > >Hi Yan > >I have developed an automatic image analysis software (www.cvistec.com > ) for complex biomedical images. It is best suited >for high throughput, high content image analysis and tissue microarrays. >Customized measurement solutions for your specific images can be add. If you >are interested, please get in touch with me. > >Kind regards >Chistof Krug > >Informatikb?ro Christof Krug >Dominikstr.21 >D-81929 M?nchen >Phone +49 89 99341972 >Mobil +49 170 5836734 >Fax +49 89 99341974 >Email info@cvistec.com >WWW www.cvistec.com > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >********************************************************************** >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient, please >delete it and notify the sender. > >Views expressed in this message and any attachments are those >of the individual sender, and are not necessarily the views of The >Children's Hospital at Westmead > >This footnote also confirms that this email message has been >virus scanned and although no computer viruses were detected, >the Childrens Hospital at Westmead accepts no liability for any >consequential damage resulting from email containing computer >viruses. >********************************************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Louise Renton >Bone Research Unit >University of the Witwatersrand >Johannesburg >South Africa >.......so what IS the speed of dark? > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From rschoon <@t> email.unc.edu Wed Mar 16 06:17:12 2005 From: rschoon <@t> email.unc.edu (rschoon) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Off topic Automated Image analysis In-Reply-To: <1DBWiu-0N1hvU0@fwd24.sul.t-online.de> References: <1DBWiu-0N1hvU0@fwd24.sul.t-online.de> Message-ID: <423823C8.2000401@email.unc.edu> Christof, If one were to, and I quote "Since I replied to a question from the _/*archive*/_ of a member of histonet" use that as an argument then there are a lot (thousands) of questions in the archives which would open up the Histonet to innumerable blatent advertizements such as yours. I certainly don't remember any recent questions on image analysis. There are many ways to get your product information out to potential customers but the way you chose is not acceptable. Robert Schoonhoven UNC - CH Christof Krug wrote: > >Sorry, forgot to send it to histonet too. >Christof > >-----Urspr?ngliche Nachricht----- >Von: Christof Krug [mailto:Christof.Krug@t-online.de] >Gesendet: Mittwoch, 16. M?rz 2005 08:43 >An: 'Tony Henwood' >Betreff: AW: [Histonet] AW: Automated Image analysis > >Hi Tony > >I carefully read the rules for histonet: > >"Vendors and those with commercial interests in histology products are >welcome contributors however,we ask that blatant advertisements be >avoided at all times. It is fine to refer to product that your company >produces if it is pertinent to a topic being discussed on the list. >Unsolicited advertisements are poorly tolerated by the members and you >will likely receive a number of negative comments if you overstep the >boundaries." > >Since I replied to a question from the archive of a member of histonet, I do >not feel this is blatant advertisement, but according to the rules. > >Regards >Christof > > >Informatikb?ro Christof Krug >Dominikstr.21 >D-81929 M?nchen >Phone +49 89 99341972 >Mobil +49 170 5836734 >Fax +49 89 99341974 >Email info@cvistec.com >WWW www.cvistec.com > >-----Urspr?ngliche Nachricht----- >Von: Tony Henwood [mailto:AnthonyH@chw.edu.au] >Gesendet: Dienstag, 15. M?rz 2005 22:02 >An: 'Christof Krug' >Cc: 'histonet@lists.utsouthwestern.edu' >Betreff: RE: [Histonet] AW: Automated Image analysis > >This seems to be a blatant addvertisement! >Christof this is not allowed on histonet > > >Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) >Laboratory Manager & Senior Scientist >The Children's Hospital at Westmead, >Locked Bag 4001, Westmead, 2145, AUSTRALIA. >Tel: 612 9845 3306 >Fax: 612 9845 3318 > > > > > >-----Original Message----- >From: Christof Krug [mailto:Christof.Krug@t-online.de] >Sent: Wednesday, 16 March 2005 1:06 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] AW: Automated Image analysis > > >Hi Yan > >I have developed an automatic image analysis software (www.cvistec.com > ) for complex biomedical images. It is best suited >for high throughput, high content image analysis and tissue microarrays. >Customized measurement solutions for your specific images can be add. If you >are interested, please get in touch with me. > >Kind regards >Chistof Krug > >Informatikb?ro Christof Krug >Dominikstr.21 >D-81929 M?nchen >Phone +49 89 99341972 >Mobil +49 170 5836734 >Fax +49 89 99341974 >Email info@cvistec.com >WWW www.cvistec.com > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >********************************************************************** >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient, please >delete it and notify the sender. > >Views expressed in this message and any attachments are those >of the individual sender, and are not necessarily the views of The >Children's Hospital at Westmead > >This footnote also confirms that this email message has been >virus scanned and although no computer viruses were detected, >the Childrens Hospital at Westmead accepts no liability for any >consequential damage resulting from email containing computer >viruses. >********************************************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Felix.Rintelen <@t> serono.com Wed Mar 16 06:25:15 2005 From: Felix.Rintelen <@t> serono.com (Felix.Rintelen@serono.com) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] T cell staining in Spleen Message-ID: Dear all, I'm trying to stain CD4 and CD8 T cells in cryosections from mouse spleen. However, with not much success so far. The good thing is that I only see staining in spleen (and not in liver, which was embedded in the same block together with the spleen). However, I don't really get staining in the areas where I would expect to see it and I only see small clusters of cells instead of big ones. And the real problem is that I get the exact same staining with the Isotype control as with the specific anti-CD4 and anti-CD8 antibodies. (I tired to post a picture of the staining on www.histonet.org, but so far I always get a message back of delivery failure, but I will try again...). Somehow it seems that IgG2a antibodies unspecifically bind to spleen but not to liver... I'm using the following antibodies that are supposed to work in immunohistochemistry: Rat anti-mouse CD4 IgG2a, Clone RM4-5, BD 550280 Rat anti-mouse CD8a IgG2a, Clone 53-6.7, BD 550281 Rat IgG2a Isotype control Clone R35-95, BD 559073 Secondary Antibody was Goat anti-Rat IgG (whole molecule) - FITC (SIGMA F-6258) The protocol that I was using is the following: Sections: 10um of mouse liver & spleen embedded in OCT medium Dry over night at room temperature Fix in 100% Aceton 4?C 10', dry 15' and then store at -80?C until use Take out of freezer and dry 5' Rehydrate 20' in PBS Block 20' with 3% normal goat serum in buffer (0.5%BSA, 0.1% Tween20 in PBS) Wash 5' in buffer Incubate with 1? Antibody (see above) 1hour (Dilutions: 1:10, 1:25, 1:50, Isotype control 1:10 and 1:50) Wash 3x 5' with buffer Incubate with 2? Antibody (see above) 1 hour (Dilution 1:25) Wash 3x 5' with buffer Mount in Fluoromount G (Electon Microscopy Sciences) I also tried to do Immunohistochemistry with the same antibodies using Vectastain Elite ABC Kit (Rat IgG) (Vector Laboratories) Peroxidase system with NovaRed as substrate and I get a similar result as with the fluorescence staining. I would very much appreciate your advice or to hear your opinion. Maybe somebody may recommend another protocol / antibodies that should work on cryostat sections. Thank you very much in advance, Best Regards, Felix ------------- Felix Rintelen (Post Doc Fellow) Serono Pharmaceutical Research Institute 14, Chemin des Aulx 1228 Plan-les-Ouates Geneva, Switzerland ----------------------------------------- S - This message contains confidential information and is intended only for the individual named. If you are not the named addressee, you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. e-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain malware. The presence of this disclaimer is not a proof that it was originated at Serono International S.A. or one of its affiliates. Serono International S.A and its affiliates therefore do not accept liability for any errors or omissions in the content of this message, which arise as a result of e-mail transmission. If verification is required, please request a hard-copy version. Serono International SA, 15bis Chemin Des Mines, Geneva, Switzerland, www.serono.com. From delventh3 <@t> aol.com Wed Mar 16 06:50:32 2005 From: delventh3 <@t> aol.com (delventh3@aol.com) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Christoff's message Message-ID: <8C6F83DDFBD9B64-C7C-1FE2@mblk-d43.sysops.aol.com> In discussing this posting we've aired this message thoroughly and certainly called more attention than the usual posting. Christoff certainly sounds like He did his homework and has respectfully replied. Can we just delete when things are not of importance to us? Priscilla From cgfields <@t> lexhealth.org Wed Mar 16 07:05:16 2005 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] CK903 w/Feulgen Message-ID: A fellow Histo tech at the VA in Columbia, SC is looking for a vendor for CK903 w/Feulgen (34BE12 w/Feulgen). He is having a problem finding this particular antibody. Any help is appreciated. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 (803) 936-8214 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From Heather.A.Harper <@t> pcola.med.navy.mil Wed Mar 16 07:10:54 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Are Gloves a CAP regulation? Message-ID: <807FE48C5A7CC940B973B58D32E7014318F9332A@nhpens-exch1.pcola.med.navy.mil> Since I work at a military facility, I have notice that all the military histo techs wear gloves to embed and cut. I went to school and we never did this. When CAP came last year, my co-worker insisted that this was a CAP regulation. Does anybody know if this is a CAP regulation, because this is news to me and I was never taught in school to wear gloves to embed or to cut. Thank you. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL From TMcNemar <@t> lmhealth.org Wed Mar 16 07:30:01 2005 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Starting ER/PR Message-ID: <6CD94D97ED7D924BA5C2B588FA952821396794@nt_exchange> Choose your stainer first. The protocols will vary from stainer to stainer. I have had the Ventana and am currently usiing the Dako. Never did ER/PR on the Ventana but they are gorgeous on the Dako (with Dako's antibodies). Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio 43055 -----Original Message----- From: Cindy DuBois [mailto:dpahisto@yahoo.com] Sent: Tuesday, March 15, 2005 6:12 PM To: Histonet Subject: [Histonet] Starting ER/PR Our doctors are considering starting ER/PR studies in our lab. I currently stain all immunos by hand, but they said they would buy a stainer if we add ER/PR to our protocols. Where do I start to obtain information on staining procedures, requirements, etc... thanks, Cindy DuBois Delta Pathology Assoc. --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kristam <@t> zoo.ufl.edu Mon Mar 14 16:27:58 2005 From: kristam <@t> zoo.ufl.edu (Krista McCoy) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] decalcifying embedded tissues Message-ID: <6.0.1.1.0.20050314165023.0274ec88@clas.ufl.edu> Hello, I am studying gonad morphology of frogs. I usually decalcify then embed the whole animal (because they are tiny). However, I recently forgot to decalcify (several samples). I have ordered and soaked many of my samples in RDO, but I am still having a hard time sectioning them. They are either scratched along the surface or "falling out" or the paraffin... Are their any suggestions on how I can de-calcyfy these specimens? Should I take them out of the block and soak them then re-embed? Thanks, Krista From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 16 08:27:45 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] processing[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0A3@bhrv-nt-11.bhrv.nwest.nhs.uk> I fancy it involved removing the paraffin and taking back to thin cedar wood oil (which is a 'clearing agent') and soaking for a few hours. Then you remove the cedar wood oil with three changes of molten paraffin. We also used 'Mollyfex' but I can't remember who sold it; the problem over taking tissue right back to water is that you have to dehydrate and clear again, all that hardens tissue. If the tissues are irretrievable then give it a try; but only after you have tried to get a diagnostic section. If you really want to sort it out then read 'Histopathologic Technic and Practical Histochemistry' RD Lillie and Harold M. FullmerMcGraw-Hill Inc 1975. Histology is becoming a bit like computers I think; I grew up with MSDOS and DRDOS and can still 'shell out' from Windows XP. Automatic tissue processors mean we are losing the 'art' in Histology; the grumblings of an old man. I suppose buying Lillie is like buying a book on MSDOS too; you never know when you need to go back to basics. Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Jennifer Sipes [mailto:jengirl1014@yahoo.com] Sent: 16 March 2005 12:34 To: Kemlo Rogerson Subject: RE: [Histonet] processing[Scanned] I'd greatly appreciate that. What is this Lillie book? I don't think I have that one. Would it be a good idea to purchase it? Thanks again for your help. Kemlo Rogerson wrote: Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com _____ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From mcauliff <@t> umdnj.edu Wed Mar 16 11:35:30 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] history of staining techs In-Reply-To: <4237FC8B.3040208@medecine.unige.ch> References: <4237FC8B.3040208@medecine.unige.ch> Message-ID: <42386E62.5050000@umdnj.edu> "History of Staining", third edition, by George Clark and Frederick Kasten, Williams and Wilkins, 1983. I don't know if there is a more recent edition. Marco Prunotto wrote: > Dear All, > > I'm post-doc in prof. Gabbiani Lab in the Geneva Pathology Dept., > Medicine Faculty. I'm also interested in history of Science. > Have you got any idea of books or articles on history of staining > techs and histological preparation in general? > > thanks a lot > > Marco Prunotto > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Heather.A.Harper <@t> pcola.med.navy.mil Wed Mar 16 08:38:19 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Reprocessing? Message-ID: <807FE48C5A7CC940B973B58D32E7014318F9334D@nhpens-exch1.pcola.med.navy.mil> I have read several emails where people talk about reprocessing. I went to histology school in 98 and graduated in Dec.99 and I was taught there is no such thing as reprocessing. This procedure only hardens the tissue more and makes it more difficult to cut. I have had the pathologists here cut some chunks of fat, and than wonder why they do not receive a good section and want to know if the tissue can be reprocessed and I tell them "NO". I slapped a nickel on the grossing bench and have told each pathologists that the sections can not be thicker than a nickel. If one can't take the time to gross in decent thin sections, than to me it's garbage in and garbage out and not to expect me to perform some miracle to get a good section. So do a lot of techs perform reprocessing? I'm curious at this procedure because I was taught that this doesn't work the majority of the time and I have even tried it a few times and to me it made matters worst. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL From Charles.Embrey <@t> carle.com Wed Mar 16 08:49:17 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Are Gloves a CAP regulation? Message-ID: I was a military histo tech and I never wore gloves when cutting or embedding. I have never seen anything in any of my CAP inspections that would require it. Have your co-worker tell you which CAP inspection checklist item addresses this. Charles Embrey -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Wednesday, March 16, 2005 7:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Gloves a CAP regulation? Since I work at a military facility, I have notice that all the military histo techs wear gloves to embed and cut. I went to school and we never did this. When CAP came last year, my co-worker insisted that this was a CAP regulation. Does anybody know if this is a CAP regulation, because this is news to me and I was never taught in school to wear gloves to embed or to cut. Thank you. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Wed Mar 16 09:12:06 2005 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Wondering... Message-ID: Hi, I haven't received anything from Histonet for a few days. Was wondering if there were problems... Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From twebster <@t> nmcinc.org Wed Mar 16 09:12:17 2005 From: twebster <@t> nmcinc.org (Tim Webster) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] (Almost) free Cytoseal 60 & Toluidine Blue O Message-ID: Hi Folks, We no longer use Cytoseal, and we can't return it. We have 7 4oz bottles going for the price of postage. We also have 6 bottles of Fisher Scientific Tol Blue O T161 Dye (25g) lot # 995514. If anyone is interested, let me know. - First come first served. Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 16 09:32:25 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Reprocessing?[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0A4@bhrv-nt-11.bhrv.nwest.nhs.uk> Re-processing, read altered processing; you can introduce elements into tissue that makes cutting easier after the blocks have been adversely affected by processing. For example remember the Shandon Processors that dangled the baskets from one pot to another? They sometimes went wrong and the tissues dried in the air after coming out of alcohol. They ended up like 'bricks' so you had to take them back to be rejuvenated. Maybe these terms have become redundant just like the TYPE command. Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Heather.A.Harper@pcola.med.navy.mil [mailto:Heather.A.Harper@pcola.med.navy.mil] Sent: 16 March 2005 14:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing?[Scanned] I have read several emails where people talk about reprocessing. I went to histology school in 98 and graduated in Dec.99 and I was taught there is no such thing as reprocessing. This procedure only hardens the tissue more and makes it more difficult to cut. I have had the pathologists here cut some chunks of fat, and than wonder why they do not receive a good section and want to know if the tissue can be reprocessed and I tell them "NO". I slapped a nickel on the grossing bench and have told each pathologists that the sections can not be thicker than a nickel. If one can't take the time to gross in decent thin sections, than to me it's garbage in and garbage out and not to expect me to perform some miracle to get a good section. So do a lot of techs perform reprocessing? I'm curious at this procedure because I was taught that this doesn't work the majority of the time and I have even tried it a few times and to me it made matters worst. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Andrew.MacDuff <@t> ed.ac.uk Wed Mar 16 09:58:36 2005 From: Andrew.MacDuff <@t> ed.ac.uk (Andrew MacDuff) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Granulocyte MPO staining Message-ID: <006001c52a41$030a61a0$b9b2d781@universi8lynpq> Hi all Does anyone have a protocol for Leder's stain for granulocyte specific esterase (or equivalent)? If so could you send me a copy Many thanks Andrew Andrew MacDuff Clinical Research Fellow Wilkie Laboratory Medical School Edinburgh University Teviot Place Edinburgh andrew.macduff@ed.ac.uk From MSafron <@t> wilresearch.com Wed Mar 16 09:59:58 2005 From: MSafron <@t> wilresearch.com (Mike Safron) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Annexin V and TUNEL Stain Message-ID: A am looking for a GLP laboratory with experience in Annexin and TUNEL Stain in the Gut associated lymphoid tissues. Can anybody offer help or suggestions. Michael Safron A.S.,HT(ASCP) Manager, Histology WIL Research Laboratories LLC 419-289-8700 ext: 3040 Ashland, Ohio 44805 From ploykasek <@t> phenopath.com Wed Mar 16 10:34:40 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] I use the Fisher Microprobe Staining system In-Reply-To: Message-ID: We use the Microprobe & love it. It's inexpensive, uses little reagent, very consistent heat & results. It does have a few quirks that you need to watch out for - be sure & use cap-gap slides, put tissue low on slides, carefully watch that your reagents drain & pick up well. It's important to have nice, thin sections. Once you get the feel of what to watch for, it's great. Be sure there are surfactants in your buffer. If you need more info, contact me. Good luck. Patti Loykasek PhenoPath Laboratories Seattle, WA > > I love the Fisher Microprobe System, it is simple and easy for a small > lab to get good consistent staining. It's reasonably priced too, and > you save money in reagents. I hope this helps- Good Luck! > > Christine Tambasco HT (ASCP) > > St. Mary's Hospital > > Amsterdam, New York 12070 > ph-518-841-7287 >> From: Kelly D Mcqueeney >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Anyone use the Fisher Microprobe Staining system? >> Date: Mon, 14 Mar 2005 14:06:29 -0500 >> >> Hi Histonetters, >> Has anyone used the Fisher Microprobe Staining system? Do you have >> any problems? We are interested in a very simple staining system for >> only one 60-minute incubation in hot ligand. I have used the Thermo >> Sequenza but we are having technical issues, plus loading the slides >> is a pain. We do not work with more than 20 slides at a time (and >> using radioligand, clean-up is an issue). I would appreciate no >> response from reps concerning complex, automated systems, we have >> already determined they are not right for us. >> >> Thanks, >> Kelly >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Wed Mar 16 10:38:53 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] T cell staining in Spleen Message-ID: In my opinion Gayle Callis is the expert in mouse CD4 and CD8 staining. I have followed her protocol with fabulous results. If she does not respond you could check the archives or let me know and I will pass on the information. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Felix.Rintelen@serono.com [mailto:Felix.Rintelen@serono.com] Sent: Wednesday, March 16, 2005 5:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] T cell staining in Spleen Dear all, I'm trying to stain CD4 and CD8 T cells in cryosections from mouse spleen. However, with not much success so far. The good thing is that I only see staining in spleen (and not in liver, which was embedded in the same block together with the spleen). However, I don't really get staining in the areas where I would expect to see it and I only see small clusters of cells instead of big ones. And the real problem is that I get the exact same staining with the Isotype control as with the specific anti-CD4 and anti-CD8 antibodies. (I tired to post a picture of the staining on www.histonet.org, but so far I always get a message back of delivery failure, but I will try again...). Somehow it seems that IgG2a antibodies unspecifically bind to spleen but not to liver... I'm using the following antibodies that are supposed to work in immunohistochemistry: Rat anti-mouse CD4 IgG2a, Clone RM4-5, BD 550280 Rat anti-mouse CD8a IgG2a, Clone 53-6.7, BD 550281 Rat IgG2a Isotype control Clone R35-95, BD 559073 Secondary Antibody was Goat anti-Rat IgG (whole molecule) - FITC (SIGMA F-6258) The protocol that I was using is the following: Sections: 10um of mouse liver & spleen embedded in OCT medium Dry over night at room temperature Fix in 100% Aceton 4?C 10', dry 15' and then store at -80?C until use Take out of freezer and dry 5' Rehydrate 20' in PBS Block 20' with 3% normal goat serum in buffer (0.5%BSA, 0.1% Tween20 in PBS) Wash 5' in buffer Incubate with 1? Antibody (see above) 1hour (Dilutions: 1:10, 1:25, 1:50, Isotype control 1:10 and 1:50) Wash 3x 5' with buffer Incubate with 2? Antibody (see above) 1 hour (Dilution 1:25) Wash 3x 5' with buffer Mount in Fluoromount G (Electon Microscopy Sciences) I also tried to do Immunohistochemistry with the same antibodies using Vectastain Elite ABC Kit (Rat IgG) (Vector Laboratories) Peroxidase system with NovaRed as substrate and I get a similar result as with the fluorescence staining. I would very much appreciate your advice or to hear your opinion. Maybe somebody may recommend another protocol / antibodies that should work on cryostat sections. Thank you very much in advance, Best Regards, Felix ------------- Felix Rintelen (Post Doc Fellow) Serono Pharmaceutical Research Institute 14, Chemin des Aulx 1228 Plan-les-Ouates Geneva, Switzerland ----------------------------------------- S - This message contains confidential information and is intended only for the individual named. If you are not the named addressee, you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. e-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain malware. The presence of this disclaimer is not a proof that it was originated at Serono International S.A. or one of its affiliates. Serono International S.A and its affiliates therefore do not accept liability for any errors or omissions in the content of this message, which arise as a result of e-mail transmission. If verification is required, please request a hard-copy version. Serono International SA, 15bis Chemin Des Mines, Geneva, Switzerland, www.serono.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Mar 16 10:52:22 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Murine CD4 and CD8 T cell staining in Spleen - long reply In-Reply-To: References: Message-ID: <6.0.0.22.1.20050316085406.01afae90@gemini.msu.montana.edu> Felix, Please send me your photo via private email, I will take a look at it without disturbing Histonet. I will be happy to visit with you privately on how to get IHC results also. Both your primary and secondary antibody concentrations are high. We do immunofluorescent (IFA) staining for CD4 and CD8 using the same antibodies you use and see definite patterns in the spleen (excellent positive control - it could be your liver does not have these cells present.) We never use Tween 20 for IFA work, only for IHC. Buffers can contain normal serum matched to host of secondary, approx 0.2% to keep section from drying out during staining. We prefer normal serum to BSA and if we had to use BSA, it is protease/immunglobulin free from Jackson. Most of the time, we keep the buffer pure, without any additives. After the secondary, we rinse 5X with pure buffer to insure there is no fluorophore sitting around - glowing garbage. Our FS are no thicker than 5 um. For IFA, we prefer to section, air dry overnight then fix with either cold acetone the next day, air dry 20 min and proceed with staining. Instead of air drying FS, storing, fixing, air drying and storing again - simply air dry frozen sections for a few hours at RT, fix in cold acetone, air dry 20 min to get rid of acetone, then store at -80C in a box containing a bag of 16 mesh silica gel to keep FS dry. Bring out acetone fixed FS on day of staining but DO NOT OPEN BOX for 30 min to allow these fixed FS to equilibrate to RT. Water condensation is the enemy, you want DRY FS. These FS will keep for a few weeks. Never take a box out of freezer, then pull a few slides for staining, as freeze/thaw is very damaging to these antigens. Use separate boxes/staining run or slide mailers in baggie with large tea bag of silica gel so you can pull slides without exposing other sections to freeze/thaw. You can get rid of some nonspecific binding by using a normal serum block of 5% goat serum/1% mouse serum, be sure the serums are heat inactivated, cooled and and microfiltered for purity/sterile conditions. We use this NSB before primary application, and for dilution of secondary. If is a good idea to do a panel dilution of your primary antibodies starting with target concentration of 10 ug/ml so you optimize your working concentration. Secondary antibody is usually used at approx 2 to 5 ug/ml, or usually about 1:250 of a 0.5 mg/ml stock concentration of secondary. It is important to purchase the goat anti rat secondary antibody adsorbed to mouse to prevent nonspecific binding. We prefer a secondary (goat antiRat, adsorbed to mouse) that is an F(ab')2 fragment of IgG to prevent fc portion of IgG molecule from binding to fc receptors on mouse tissues although whole IgG molecule secondaries will work. Excellent secondaries come from Jackson ImmunoResearch, Biosource/TAGO, Rockland and Southern Biotechnology for murine work. Jackson provides recommended concentration/dilution of their secondaries for immunostaining work (IFA/IHC? on their specification sheets. You can use TRIS buffered saline or Dulbecco PBS containing 0.2% normal serum matched to host of secondary, and do not add Tween 20, not needed for IFA work. Normal serum block 30 min at RT. For IFA work, we tend to work with a slightly higher concentration of primary as compared to our IHC with the same primaries you work with. For IFA work, try diluting your CD4 ((0.5mg/ml) 1:200 to 1:250, and dilute in 2% to 5% goat serum. For negative control the IgG2a must be the same concentration as your CD4 primary. For CD8 (0.5mg/ml) , we dilute 1:50 in diluent as CD4 (for CD8 IHC work, we dilute 1:100) IgG2a must be same concentration for CD8 negative control Incubation is 30 min at RT Secondary goat antiRat - FITC is diluted 1:250 to 1:300 in 5% goat, spun down to get rid of fluorophore protein aggregates before application to section and incubated at RT for 30 min IN THE DARK. Rinse well, coverslip. We prefer Molecular Probes antiFade, Prolong Gold ready to use mounting media to prevent photobleaching of FITC aothough your media is probably adequate. At 05:25 AM 3/16/2005, you wrote: >Dear all, > >I'm trying to stain CD4 and CD8 T cells in cryosections from mouse spleen. >However, with not much success so far. The good thing is that I only see >staining in spleen (and not in liver, which was embedded in the same block >together with the spleen). However, I don't really get staining in the >areas where I would expect to see it and I only see small clusters of cells >instead of big ones. And the real problem is that I get the exact same >staining with the Isotype control as with the specific anti-CD4 and >anti-CD8 antibodies. (I tired to post a picture of the staining on >www.histonet.org, but so far I always get a message back of delivery >failure, but I will try again...). Somehow it seems that IgG2a antibodies >unspecifically bind to spleen but not to liver... > >I'm using the following antibodies that are supposed to work in >immunohistochemistry: >Rat anti-mouse CD4 IgG2a, Clone RM4-5, BD 550280 >Rat anti-mouse CD8a IgG2a, Clone 53-6.7, BD 550281 >Rat IgG2a Isotype control Clone R35-95, BD 559073 > >Secondary Antibody was Goat anti-Rat IgG (whole molecule) - FITC (SIGMA >F-6258) > >The protocol that I was using is the following: > >Sections: 10um of mouse liver & spleen embedded in OCT medium >Dry over night at room temperature >Fix in 100% Aceton 4?C 10', dry 15' and then store at -80?C until use > >Take out of freezer and dry 5' >Rehydrate 20' in PBS >Block 20' with 3% normal goat serum in buffer (0.5%BSA, 0.1% Tween20 in >PBS) >Wash 5' in buffer >Incubate with 1? Antibody (see above) 1hour (Dilutions: 1:10, 1:25, 1:50, >Isotype control 1:10 and 1:50) >Wash 3x 5' with buffer >Incubate with 2? Antibody (see above) 1 hour (Dilution 1:25) >Wash 3x 5' with buffer >Mount in Fluoromount G (Electon Microscopy Sciences) > >I also tried to do Immunohistochemistry with the same antibodies using >Vectastain Elite ABC Kit (Rat IgG) (Vector Laboratories) Peroxidase system >with NovaRed as substrate and I get a similar result as with the >fluorescence staining. > >I would very much appreciate your advice or to hear your opinion. Maybe >somebody may recommend another protocol / antibodies that should work on >cryostat sections. > >Thank you very much in advance, > >Best Regards, > >Felix > >------------- > >Felix Rintelen (Post Doc Fellow) >Serono Pharmaceutical Research Institute >14, Chemin des Aulx >1228 Plan-les-Ouates >Geneva, Switzerland > > > >----------------------------------------- >S - This message contains confidential information and is intended only for >the individual named. If you are not the named addressee, you should not >disseminate, distribute or copy this e-mail. Please notify the sender >immediately by e-mail if you have received this e-mail by mistake and >delete this e-mail from your system. e-mail transmission cannot be >guaranteed to be secure or error-free as information could be intercepted, >corrupted, lost, destroyed, arrive late or incomplete, or contain malware. >The presence of this disclaimer is not a proof that it was originated at >Serono International S.A. or one of its affiliates. Serono International >S.A and its affiliates therefore do not accept liability for any errors or >omissions in the content of this message, which arise as a result of e-mail >transmission. If verification is required, please request a hard-copy >version. Serono International SA, 15bis Chemin Des Mines, Geneva, >Switzerland, www.serono.com. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From vazquezr <@t> ohsu.edu Wed Mar 16 10:55:27 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Are Gloves a CAP regulation? Message-ID: I agree with Charles, I too was a military (lab)histo tech and also in the private sector and have gone through several CAP inspections and have never been told to wear gloves. Robyn OHSU >>> "Charles.Embrey" 03/16/05 6:49 AM >>> I was a military histo tech and I never wore gloves when cutting or embedding. I have never seen anything in any of my CAP inspections that would require it. Have your co-worker tell you which CAP inspection checklist item addresses this. Charles Embrey -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Wednesday, March 16, 2005 7:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Gloves a CAP regulation? Since I work at a military facility, I have notice that all the military histo techs wear gloves to embed and cut. I went to school and we never did this. When CAP came last year, my co-worker insisted that this was a CAP regulation. Does anybody know if this is a CAP regulation, because this is news to me and I was never taught in school to wear gloves to embed or to cut. Thank you. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Mar 16 10:57:07 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:46 2005 Subject: [Histonet] Re: Are Gloves a CAP regulation? In-Reply-To: <807FE48C5A7CC940B973B58D32E7014318F9332A@nhpens-exch1.pcol a.med.navy.mil> References: <807FE48C5A7CC940B973B58D32E7014318F9332A@nhpens-exch1.pcola.med.navy.mil> Message-ID: <6.0.0.22.1.20050316095309.01b6bb50@gemini.msu.montana.edu> Even if it isn't a CAP regulation, gloves are a good idea when embedding and sectioning considering all the various diseases (prion?) you might run into without prior knowledge - we are finding this true of many animal projects also which do not come under the auspices of CAP. At 06:10 AM 3/16/2005, you wrote: > Since I work at a military facility, I have notice that all the military >histo techs wear gloves to embed and cut. I went to school and we never did >this. When CAP came last year, my co-worker insisted that this was a CAP >regulation. Does anybody know if this is a CAP regulation, because this is >news to me and I was never taught in school to wear gloves to embed or to >cut. Thank you. > > > >Heather A. Harper > >Supervisor of Histology/Morgue > >Naval Hospital of Pensacola, FL > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From MSafron <@t> wilresearch.com Wed Mar 16 11:03:11 2005 From: MSafron <@t> wilresearch.com (Mike Safron) Date: Fri Sep 16 15:24:46 2005 Subject: Re .[Histonet] decalcifying embedded tissues Message-ID: Krista, You will need to run these specimens back to alcohol or water to get them to decal properly. They will not decalcify properly with paraffin still infiltrated in the blocks. Michael Safron A.S.,HT(ASCP) Manager, Histology WIL Research Laboratories LLC 419-289-8700 ext: 3040 Ashland, Ohio 44805 From Nancy.Temple <@t> ssfhs.org Wed Mar 16 12:09:35 2005 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Reprocessing? Message-ID: <4862D47C7409844E9E52532B256090620101542F@ontexind01.ssfhs.org> We have reprocessed tissue blocks many times and have good success with it. Actually, the tissue cuts very well after reprocessing. We have done it 2 different ways. If the tissue has not been properly processed the first time (and these are usually fatty pieces of tissue), we melt the tissue block and run the tissue cassette through the cleaning cycle on our VIP processor. Put the cassette back into formalin and process again. We have also melted the paraffin block, blotted the tissue, and put the cassette directly into the formalin basket to be processed again. Both techniques worked just fine for us, and the tissue cut beautifully. Nancy Temple HT(ASCP) Histology Supervisor St. Francis Hospital Indianapolis, In -----Original Message----- From: Heather.A.Harper@pcola.med.navy.mil [mailto:Heather.A.Harper@pcola.med.navy.mil] Sent: Wednesday, March 16, 2005 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing? I have read several emails where people talk about reprocessing. I went to histology school in 98 and graduated in Dec.99 and I was taught there is no such thing as reprocessing. This procedure only hardens the tissue more and makes it more difficult to cut. I have had the pathologists here cut some chunks of fat, and than wonder why they do not receive a good section and want to know if the tissue can be reprocessed and I tell them "NO". I slapped a nickel on the grossing bench and have told each pathologists that the sections can not be thicker than a nickel. If one can't take the time to gross in decent thin sections, than to me it's garbage in and garbage out and not to expect me to perform some miracle to get a good section. So do a lot of techs perform reprocessing? I'm curious at this procedure because I was taught that this doesn't work the majority of the time and I have even tried it a few times and to me it made matters worst. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________ __________________________________ The information contained in this email and any accompanying documents is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by e-mail and destroy all copies of the original message. Thank you. From lori.langiano <@t> medtronic.com Wed Mar 16 12:17:22 2005 From: lori.langiano <@t> medtronic.com (Langiano, Lori, MS, HT) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] endothelial cell uptake of eosin Y in fresh tissue Message-ID: <52E5C33DF6727A448293BEE0B19D01DF01341C07@STSM1BMSGM01> Hello, does anyone have statistics on the affinity of eosin Y binding to endothelial cells, specifically denuded endothelial cells of arteries? Thanks in advance. Lori From mprice26 <@t> juno.com Wed Mar 16 12:26:01 2005 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Are Gloves a CAP regulation? Message-ID: <20050316.102619.16035.212642@webmail27.nyc.untd.com> The requirement of wearing gloves is more of a safety issue required by OSHA. Check your CAP checklist under general safety to see if it says anything about wearing gloves. I personally have never worn gloves while embedding and cutting because there is little risk of getting anything from formalin fixed/ paraffin embedded tissue. Marsha Price From LLElby <@t> LancasterGeneral.org Wed Mar 16 12:30:55 2005 From: LLElby <@t> LancasterGeneral.org (Elby, Lynette L) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] charging Message-ID: Hi Everyone, I would like to know how everyone is chrging for immuno's. See below for an exanple: S05-1234 A, B, C for S100 and Pankeratin. Do you charge for two or do you charge for six? (assuming that A, B, C is froom the first container.) Second............How would you charge if A, B, C is from three separate containers? Our instrument is the Ventana Benchmark. Please advise...Thank you. Lynette Elby Histology Supervisor Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5065 (717)544-5138 fax (717)305-6144 pager From HornHV <@t> archildrens.org Wed Mar 16 12:57:06 2005 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] charging Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6322BABE@EMAIL.archildrens.org> From the same container, only 2. From 3 separate containers, 6. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital - Changing Children's Lives Phone - 501.364.4240 Fax - 501.364.3912 Visit us on the web at www. archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elby, Lynette L Sent: Wednesday, March 16, 2005 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] charging Hi Everyone, I would like to know how everyone is chrging for immuno's. See below for an exanple: S05-1234 A, B, C for S100 and Pankeratin. Do you charge for two or do you charge for six? (assuming that A, B, C is froom the first container.) Second............How would you charge if A, B, C is from three separate containers? Our instrument is the Ventana Benchmark. Please advise...Thank you. Lynette Elby Histology Supervisor Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5065 (717)544-5138 fax (717)305-6144 pager _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From lfidgen <@t> vt.edu Wed Mar 16 13:19:19 2005 From: lfidgen <@t> vt.edu (Laura Fidgen) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] immunocytochemistry and IHC Message-ID: <6.0.0.22.0.20050316141355.02529ba8@pop.vt.edu> Good afternoon! We are a small lab trying to begin an IHC and immunocytochemistry program. Our pathologists have given us a list of various antibodies they would like to have and my question is, what is the best way to begin? There are various leukocyte markers, intermediate filaments, cytokeratins, hormones, immunoglobulins, and an "other" category proposed and I could use help getting started and pointed in the right direction. Almost all the MT's and HT's here have never been exposed to immunos. Any help would be GREATLY appreciated. Laura L. Fidgen, MScF, BSc, HT(ASCP) Laboratory and Research Practioner VMRCVM, Histopatholgy/Clinical Pathology Dept. Blacksburg, VA 24060-0443 From haldana <@t> unimoron.edu.ar Wed Mar 16 14:28:09 2005 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] immuno in block Message-ID: <002001c52a66$b32b0920$a904a8c0@um.edu> Dears I need to make immunos in the whole retinas. Can anybody have a protocol to do it? Or a similar technique in whole embryos. Times, concentrations or any advices to make Immunohistochemistry in thick tissues. Thanks in advance Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html From RizoC <@t> chi.osu.edu Wed Mar 16 14:52:28 2005 From: RizoC <@t> chi.osu.edu (Rizo, Christian) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Pathologists Assistant Position Message-ID: <979FF5962E234F45B06CF0DB7C1AABB201FEA4F6@chi2k3ms01.columbuschildrens.net> Good Day! My name is Christian M. Rizo. I am the Manager of the Anatomic Pathology Lab at Children's Hospital in Columbus Ohio. The reason for this e-mail is that I wanted to inform you that we have an opening for a Pathologists' Assistant at our institution. The position is a full-time, exempt position, dayshift with some weekends on-call. Applicants must be a Master's Degree in biological or allied health sciences graduate with three years of anatomical experience OR Bachelor's Degree in biological or allied health sciences graduate with five years of anatomical experience. Candidates who are certified by the AAPA are preferred. This position is responsible for some of the functions normally performed by the anatomic pathologists. Duties include autopsies, photography and grossing of surgical specimens. Duties also may change depending on workload and as assigned by the Vice Chair of the Anatomic Pathology Department. Applicants must have good customer relations, and technical competencies. Teamwork, dependability and communication are necessary for successful candidates. Children's Hospital Columbus Founded by a determined group of women in 1892, Children's Hospital began as a local charity to serve a dozen very ill children. Throughout the following century, this tiny community-funded mission matured into a health care system that today spans the Midwest as one of its preferred providers of pediatric health care. Columbus Children's today is ranked as one of the nation's ten largest children's hospitals and pediatric research centers. Our hospital campus has nearly 700,000 patient visits every year. Located just minutes from downtown Columbus, Children's Hospital is one of the nation's most progressive and sophisticated health care institutions. Specialty areas include * Surgery * Heart transplant/cardiac care * Cancer * Trauma * Rehabilitation * Dialysis * Bone Marrow Transplant * Neurosciences * Research Children's is also the region's only pediatric Level 1 Trauma Center. With over 1.5-million square feet of interior space, the campus at Children's Hospital is vast. * The main hospital is home to the emergency department (one of the busiest pediatric emergency departments in the nation), surgery suites, interventional radiology and all inpatient units. * In August 2005, a new clinical expansion will be established which includes 14 additional state-of-the-art operating suites (which include intra-operative MRI and robotic technologies), new space for the Children's Heart Center to include catheterization laboratories and an additional 28-bed neonatal intensive care unit as well as integrated clinical laboratory services Thank you for your time. Please forward the e-mail to personnel who you think will benefit from it. Christian M. Rizo MBA, HTL (ASCP) Manager, Anatomic Pathology Childrens Hospital 700 Childrens Drive Columbus, Ohio 43205 614.722.5465 RizoC@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From akbitting <@t> geisinger.edu Wed Mar 16 14:54:13 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Are Gloves a CAP regulation? Message-ID: I just had this argument today with our Safety & Hygiene officer. We've had quite a few cuts lately on scalpels and knives and he came in to watch out "process" and help us "improve it". He asked why some of us wear gloves and others don't. It turned into a battle-royal. (Anyway, gloves have nothing to do with preventing cuts.)I told him it is recommended but not yet "required". If anyone out there knows of a regulation stating that gloves must be worn when embedding and cutting blocks, I'd like to see it. Or, if anyone has information on the number of Histotechs who have contracted HIV or Creutzfeld-Jakob from a paraffin block, send it to me. Until then, I will remain gloveless! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> 03/16/05 8:10 AM >>> Since I work at a military facility, I have notice that all the military histo techs wear gloves to embed and cut. I went to school and we never did this. When CAP came last year, my co-worker insisted that this was a CAP regulation. Does anybody know if this is a CAP regulation, because this is news to me and I was never taught in school to wear gloves to embed or to cut. Thank you. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Stephen.J.Scholz <@t> osfhealthcare.org Wed Mar 16 14:56:42 2005 From: Stephen.J.Scholz <@t> osfhealthcare.org (Scholz, Stephen J.) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Thin-Prep Message-ID: <7F1312711CA7474A89B3DF8BA0BA54D0F5F1AA@pmc-rfd-mx01.intranet.osfnet.org> Hello all; I was instructed by a Pathologist that I work for to investigate using the ThinPrep technology in a non-gyne capacity. Is this done? I know that it is used for gyne specimens but I have never heard of using the technology for non-gyne. I'm a Histo-tech and my experience with cytology is limited so any information that I could get would be appreciated. (where to get the instrument, cost, is it applicable) Thank you, Stephen J. Scholz HT(ASCP) 815-395-5410 From cgfields <@t> lexhealth.org Wed Mar 16 15:01:34 2005 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] CK903 w/Feulgen Message-ID: Thank you...I will pass it on. Carole -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Wednesday, March 16, 2005 3:59 PM To: Carole Fields Subject: Re: [Histonet] CK903 w/Feulgen Feulgen is not an antibody. It stains DNA by the negative PO4 groups of the DNA reacting with the positively charged nitrogen groups of the dye. I've never seen a 34BE12 with a Feulgen, but I bet it's pretty. Patti Loykasek PhenoPath Laboratories Seattle, WA> A fellow Histo tech at the VA in Columbia, SC is looking for a vendor for > CK903 w/Feulgen (34BE12 w/Feulgen). He is having a problem finding this > particular antibody. Any help is appreciated. > > Carole Fields, HT,ASCP > Pathology Supervisor > Lexington Medical Center > 2720 Sunset Blvd. > W. Columbia, SC 29169 > > (803) 936-8214 > > > _________________________________ > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed above if one is provided. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From settembr <@t> umdnj.edu Wed Mar 16 14:46:24 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] immunocytochemistry and IHC Message-ID: Laura, I highly recommend that you contact DakoCytomation. They helped our lab with many aspects of immunohistochemistry. I often have people tell me that my immunos are one of the best they've seen and I have to hand if to Dako for all their help and great antibodies. Their antibodies work really nicely and incubation times can be as short as 15 minutes. Most of mine are. Call them and ask them who your representative is 1-800-235-5743. They have a great customer service team and their technical service team is so user friendly. I always had my Rolodex open to their number so I could ask them anything. I have also been to a small basic immunohistochemistry seminar in my area that was set up by Dako. Your lab could benefit from something like that too, if they offer it in your area. You can use their "ready to use" antibodies or use their concentrated ones. You can do them by hand or get automated. Dako could help you with either. Good Luck Dana Settembre, HT 1-973-972-5232 University Hospital, UMDNJ Newark, NJ >>> Laura Fidgen 3/16/2005 2:19:19 PM >>> Good afternoon! We are a small lab trying to begin an IHC and immunocytochemistry program. Our pathologists have given us a list of various antibodies they would like to have and my question is, what is the best way to begin? There are various leukocyte markers, intermediate filaments, cytokeratins, hormones, immunoglobulins, and an "other" category proposed and I could use help getting started and pointed in the right direction. Almost all the MT's and HT's here have never been exposed to immunos. Any help would be GREATLY appreciated. Laura L. Fidgen, MScF, BSc, HT(ASCP) Laboratory and Research Practioner VMRCVM, Histopatholgy/Clinical Pathology Dept. Blacksburg, VA 24060-0443 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TillRenee <@t> uams.edu Wed Mar 16 15:36:13 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] negative controls Message-ID: What is a good company to get IgG for negative controls? I need rabbit, mouse and goat. The places I've checked so far have been so different as far as quantity and price. And many don't have goat or only offer is in tiny amounts. We do mostly rabbit and mouse primaries, but a lot of our harder to find antibodies end up being goat. Renee' Till Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From kelly.mcqueeney <@t> bms.com Wed Mar 16 15:42:15 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] negative controls In-Reply-To: References: Message-ID: <4238A837.3010106@bms.com> Renee, Try Vector and Jackson for rabbit IgG. You must make small aliquots and freeze them. After using an aliquot, throw thw rest away, For some reason, rabbit IgG gives high background if it's not fresh out of the freezer. Good luck! Till, Renee wrote: >What is a good company to get IgG for negative controls? I need rabbit, >mouse and goat. The places I've checked so far have been so different as >far as quantity and price. And many don't have goat or only offer is in >tiny amounts. We do mostly rabbit and mouse primaries, but a lot of our >harder to find antibodies end up being goat. > > > >Renee' Till > > >Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JWEEMS <@t> sjha.org Wed Mar 16 16:02:29 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Thin-Prep Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA456A0@sjhaexc02.sjha.org> http://cytyc.com/ Above is the web site for Cytyc - a most reliable company for this process. I'm not sure how you would use it for tissue, but you could check them out. Good luck, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scholz, Stephen J. Sent: Wednesday, March 16, 2005 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thin-Prep Hello all; I was instructed by a Pathologist that I work for to investigate using the ThinPrep technology in a non-gyne capacity. Is this done? I know that it is used for gyne specimens but I have never heard of using the technology for non-gyne. I'm a Histo-tech and my experience with cytology is limited so any information that I could get would be appreciated. (where to get the instrument, cost, is it applicable) Thank you, Stephen J. Scholz HT(ASCP) 815-395-5410 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From TJJ <@t> Stowers-Institute.org Wed Mar 16 16:02:52 2005 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Missouri Society for Histotechnology 2005 Spring meeting Message-ID: On May 20-21, 2005 the Missouri Society for Histotechnology will host the Spring Conference at the beautiful Lodge of Four Seasons in Lake Ozark, MO. We invite you to participate in a unique learning experience. The program is outstanding this year and should have something for everyone. In addition there will be social activities and vendor displays. We look forward to seeing you. Friday, May 20, 2005 7:30 - 8:00 Registration 8:15 - 8:30 President Message - Sharon Ann Walsh 8:30 - Noon SCIENTIFIC SESSIONS: Jan Minshew, Leica Microsystems - "Ergonomics in the Workplace" Dr. Michael Graham, St Louis University - "Case Studies in Forensic Pathology" COFFEE BREAKS AND EXHIBITS Jerry Fredenburgh "Histochemistry of Special Stains" Konnie Zietner - Region V update 12:00 - 1:00 AWARDS LUNCHEON WORKSHOPS 1:30 - 4:30 3:00 - 3:30 COFFEE BREAKS AND EXHIBITS Workshop #1 - Theory of Routine & Microwave Fixation - Jerry Fredenburgy & Skip Brown Workshop #2 - A Tour Guide to Immunohistochemistry: How to get there and what to do when you arrive - Chris Moore, Ventana Medical Systems, Inc. Workshop #3 - Patient care in the Histology Lab: A Two-Way street between Histotechnologist and Pathologist - A. Weldon Schott, DO, WCP Laboratories, St. Louis, MO 6:00 - 8:00 Cruise on the "Lake of the Ozarks" - Hors d'Oeuvres provided, Cash Bar available. Visit with exhibitors and friends. Saturday, May 21, 2005 7:30 - 8:00 Registration 8:00 - 8:15 Saturday Welcome - Linda Collins-Weeks, VP MSH 8:15 - Noon SCIENTIFIC SESSIONS: Konnie Zietner, Neb. Medical Center - Creutzfeldt-Jakob Disease: Tissue Handling and Testing in the United Kingdom Dr. Sharad Mathur, VA Medical Center Kansas City, MO - Histologic Techniques in Hematopathology COFFEE BREAKS AND EXHIBITS Skip Brown, Lab Management Consultants, St. Louis, MO - Applications for Microwave Decalcification Procedures WORKSHOPS 1:30 - 4:30 Workshop #4 - Quality and Skill in Microtomy Technique, Jan Minshew, Leica Microsystems, Inc. Workshop #5 - Special Stains: The Once and Future King? - Chris Moore, Ventana Medical Systems, Inc. Workshop #6 - From Bench Tech to Management - Konnie Zietner, Neb. Medical Center. For additional information, contact: Sharon Ann Walsh - Conference Coordinator: userwalsh@aol.com Skip Brown - Educational Coordinator: skiplmcbrown@aol.com Teri Johnson - Exhibit Coordinator: mshexhibit@hotmail.com Rosetta Barkley - Registration Coordinator: rbarkley2@kumc.edu Hope to see you there! From JWEEMS <@t> sjha.org Wed Mar 16 16:39:32 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Thin-Prep Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA456AC@sjhaexc02.sjha.org> I just reread this and realized you weren't talking about tissue - duh. Let me try this again. The majority of our cytology is nons. We spin, vortex, pour off, pour in - etc. There are non-gyn supplies separate from the gyn for this purpose. If you'd like, I'll direct you to my Cytotech... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scholz, Stephen J. Sent: Wednesday, March 16, 2005 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thin-Prep Hello all; I was instructed by a Pathologist that I work for to investigate using the ThinPrep technology in a non-gyne capacity. Is this done? I know that it is used for gyne specimens but I have never heard of using the technology for non-gyne. I'm a Histo-tech and my experience with cytology is limited so any information that I could get would be appreciated. (where to get the instrument, cost, is it applicable) Thank you, Stephen J. Scholz HT(ASCP) 815-395-5410 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From gcallis <@t> montana.edu Wed Mar 16 17:14:55 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] RE: IgG negative control sources for all species In-Reply-To: References: Message-ID: <6.0.0.22.1.20050316160702.01b3a358@gemini.msu.montana.edu> Renee, Jackson ImmunoReasearch. They have all species IgG's even Golden syrian hamster, but NOT Armenian hamster, you have to do to BD Pharmingen for latter. They go a long way as they are very concentrated, and you can get them conjugated to biotin (a favorite for our for biotinylated primary control), FITC, and so on. Not terribly expensive as they go a longgggg way since their concentrations are always around 10 mg/ml or more. Also Jackson's shipping will not kill your budget, very reseasonable. Another source is Rockland Immunologicals, you can purchase these through VWR, shipping is free if you have any term contract with them. , At 02:36 PM 3/16/2005, you wrote: >What is a good company to get IgG for negative controls? I need rabbit, >mouse and goat. The places I've checked so far have been so different as >far as quantity and price. And many don't have goat or only offer is in >tiny amounts. We do mostly rabbit and mouse primaries, but a lot of our >harder to find antibodies end up being goat. > >Renee' Till Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From jkiernan <@t> uwo.ca Thu Mar 17 00:16:04 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Bubbles (Was Re: coverslippers). Also DPX References: Message-ID: <423920A4.B0A584D4@uwo.ca> Could your bubble trouble be from the medium rather than the machine? We have used cytoseal mounting media, which are poly(methylmethacrylate) with different viscosities, for several years. We apply coverslips manually. Bubbles that form near the edge of the coverslip are a regular occurrence, but it doesn't matter unless they occlude the specimen. The low price and ease of application of this mountant, which is also non-fluorescent, largely offset this bubble problem. A cooler hotplate suppresses or minimizes the bubble formation. Bubbles (other than from human error) never happened when we used DPX, a polystyrene-based mountant. DPX bought since about 1985 has contained tiny transparent droplets that can really get in the way when looking at sections more than 10-20um thick. When this problem arose, our suppliers recommended using entellan instead. Entellan is an expensive poly(methylmethacrylate) mountant that comes in a glass bottle - more troublesome to apply than squeezing cytoseal out of a plastic bottle with a narrow nozzle. With entellan we didn't get the new-DPX droplets or the bubbles at the edges of the coverslip that often occur with cytoseal. DPX was a published, non-secret resinous mountant that was almost perfect. Making it in-house was never a serious option because you could not easily buy the polystyrene ingredient from ordinary lab supply companies. We relied on vendors. The product from BDH was excellent in every way. Does anyone know a current source of DPX that meets the published (early 1950s) specs? It needs to be visibly transparent (not yellowy), devoid of autofluorescence, without microdrops that show up in thick preparations. Patricia Traphagen's bubbles may originate from a subtle imbalance between the volatility of the clearing agent and the viscosity of the mountant. Just a few thoughts to tickle up some more discussion! John Kiernan London, Canada. ------------------------------------------------------ "Traphagan, Patricia" wrote: > > We are having major air bubble problems after we have coversliped our > slides using the Leica coverslipper. We use Cytoseal and Richard Allen > coverslippers. We set the dials at 2.5 volume and 1 mountant type. > > Patty Traphagan > Histology Technical Specialist > Methodist Hospital Laboratory > 952-993-2823 or 952-993-5304 > > PRIVACY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain business confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If this e-mail was not intended for you, please notify the sender by reply e-mail that you received this in error. Destroy all copies of the original message and attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Thu Mar 17 02:00:33 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Thin-Prep[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0A6@bhrv-nt-11.bhrv.nwest.nhs.uk> We regularly use ThinPrep (on a T2000) for our Non-Gynae on one Site; Cytospins on the other. I believe all the instructions of use are in the manuals that accompany the machine. From my short stay here, everything seems fine but I have, as yet, not compared the two systems, but I shall; we will have to migrate to one technology. I screened a few urines and pleural fluids prepared by this technology whilst I worked in the Independent Sector in London, they seemed very good, but I had limited experience. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: 16 March 2005 22:40 To: Scholz, Stephen J.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thin-Prep[Scanned] I just reread this and realized you weren't talking about tissue - duh. Let me try this again. The majority of our cytology is nons. We spin, vortex, pour off, pour in - etc. There are non-gyn supplies separate from the gyn for this purpose. If you'd like, I'll direct you to my Cytotech... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scholz, Stephen J. Sent: Wednesday, March 16, 2005 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thin-Prep Hello all; I was instructed by a Pathologist that I work for to investigate using the ThinPrep technology in a non-gyne capacity. Is this done? I know that it is used for gyne specimens but I have never heard of using the technology for non-gyne. I'm a Histo-tech and my experience with cytology is limited so any information that I could get would be appreciated. (where to get the instrument, cost, is it applicable) Thank you, Stephen J. Scholz HT(ASCP) 815-395-5410 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From mab70 <@t> medschl.cam.ac.uk Thu Mar 17 03:20:38 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] immuno in block Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114AE@mius2.medlan.cam.ac.uk> I used to do this in skin sheets, I have also stained isolated hair follicles. I think your samples should fit into 6 well plates. I used to incubate overnight at 4C for the primary antibody, otherwise the method is very similar to that for sections. Remember to use plenty of each reagent so that your samples remain immersed at all times. You can transfer the reagents with pasteur pipettes or if your tissue is robust enough transfer it to a new well. When I stained hair follicles, I used a 3:1 solution of ethanol:acetic acid to permeabilise the samples. I would optimise the staining on frozen sections prior to starting on the whole mounts. I have no experience of staining whole embryos, so I'm not sure how well your reagents will penetrate the samples. No doubt someone else will give you an answer. Good luck Margaret -----Original Message----- From: Hernan Aldana Marcos [mailto:haldana@unimoron.edu.ar] Sent: Wednesday, March 16, 2005 8:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] immuno in block Dears I need to make immunos in the whole retinas. Can anybody have a protocol to do it? Or a similar technique in whole embryos. Times, concentrations or any advices to make Immunohistochemistry in thick tissues. Thanks in advance Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sakima <@t> bigpond.net.au Thu Mar 17 06:17:49 2005 From: sakima <@t> bigpond.net.au (Satoshi Akima) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] positive controls - adequacy? Message-ID: <81a80b730441eeff9e5235625622bf8d@bigpond.net.au> Dear Listmembers, this is my first post on the list and I was hoping to canvass some opinions. I am doing some research and would like to stain for neutrophils, lymphocytes and macrophages (to see which cell type seems to have a pathogenic role). The question is what is good enough as a positive control, especially when you are using commercial antibodies for which a published body of literature supporting the specificity of the antibody is out there. Would you: A. Just use a blood smear (whole blood) B. Use white cells from the Buffy coat C. Use a column (like the miniMACS system using antibody couples magnetic beads) to separate out lineages D. In the case of mononuclear cells use Lymphoprep to separate out PBMCs E. Culture your PBMCs to get a purer isolate of macrophages Option C is nice but would cause a big time blow out in the budget - all just to get a positive control for IHC. Seems a bit over the top. I'm also in a hurry to get my paper out and time management is an issue (reagents take time to order in from overseas). What do people think? Thanks for your help. Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia From NLemke <@t> asterand.com Thu Mar 17 08:28:56 2005 From: NLemke <@t> asterand.com (Nancy Lemke) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] training Message-ID: <56E63440ABF1FC4897947D5BA4CFA44F04A9E14E@ATL1VEXC005.usdom004.tco.tc> Histonetters, Can any or all of you tell me which immunostainer companies offer in house training included with the purchase of an instrument? I know that Dako does but do not know if any of the other companies do. Thanks in advance, Nancy Lemke Asterand, Inc. TechOne 440 Burroughs Detroit, MI 48202 This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. From NLemke <@t> asterand.com Thu Mar 17 09:37:22 2005 From: NLemke <@t> asterand.com (Nancy Lemke) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] training...again Message-ID: <56E63440ABF1FC4897947D5BA4CFA44F04A9E1A7@ATL1VEXC005.usdom004.tco.tc> Hello, I would like to know which companies offer both on site and in house(company) immunostainer training. Thanks again, Nancy Lemke Asterand, Inc. TechOne 440 Burroughs Detroit, MI 48202 This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. From DDDeltour <@t> mar.med.navy.mil Thu Mar 17 09:53:06 2005 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] training...again Message-ID: <080566D001A3D9459FFC0A391A646C9104BEF88D@marxchg03.mar.med.navy.mil> Ventana paid for our tech to go to Arizona for a week. Doug -----Original Message----- From: Nancy Lemke [mailto:NLemke@asterand.com] Sent: Thursday, March 17, 2005 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] training...again Hello, I would like to know which companies offer both on site and in house(company) immunostainer training. Thanks again, Nancy Lemke Asterand, Inc. TechOne 440 Burroughs Detroit, MI 48202 This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhawkins <@t> UTMB.EDU Thu Mar 17 10:06:55 2005 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] cassettes Message-ID: <8D6F233E2A5D574B929F3944F3316FD002BD88E6@EXCH2K3.utmb.edu> We are planning a multicenter study and would like to use histology embedding cassettes that are distinct from all the colors that are normally used at each institution. Does anyone know of a supplier of cassettes of unusual or distinctive colors or custom colors? Thanks. Hal Hawkins, UT Medical Branch, Galveston, TX From Julie.Sanders <@t> med.va.gov Thu Mar 17 10:10:55 2005 From: Julie.Sanders <@t> med.va.gov (Julie.Sanders@med.va.gov) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] uneven sections on slides Message-ID: <457381D92B01BD44B21CF37CC02EBDFD029275B3@vhacinexc5.v10.med.va.gov> Hello All, In the past few days one our pathologists has been complaining about "uneven sections." In other words, he's having to focus up and down a lot because the sections aren't flat on the slide. Any body have any explanations of why this could be happening and any solutions? We haven't been doing anything different, use plus charged slides and paraplast x-tra paraffin. 15 minutes in a 55 degree C oven and automatic stainer. Any comments are greatly appreciated. Julie Julie Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology VA Medical Center Cincinnati, Oh. 45220 Julie A. Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC From Julie.Sanders <@t> med.va.gov Thu Mar 17 10:34:47 2005 From: Julie.Sanders <@t> med.va.gov (Julie.Sanders@med.va.gov) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 28 Message-ID: <457381D92B01BD44B21CF37CC02EBDFD029275B5@vhacinexc5.v10.med.va.gov> Stephen, We use the Thin Prep for non gyn specimens. Do you already have this machine? If so, call Cytec and I am sure they could send someone to show you how to process non-gyn. Also in the procedure manual provided with the machine is a step by step procedure for all types of non-gyn specimens. Julie Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VA Medical Center Cincinnati, Ohio 45220 Hello all; I was instructed by a Pathologist that I work for to investigate using the ThinPrep technology in a non-gyn capacity. Is this done? I know that it is used for gyn specimens but I have never heard of using the technology for non-gyn. I'm a Histo-tech and my experience with cytology is limited so any information that I could get would be appreciated. (where to get the instrument, cost, is it applicable) Thank you, Stephen J. Scholz HT(ASCP) 815-395-5410 From RCHIOVETTI <@t> aol.com Thu Mar 17 10:54:09 2005 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] uneven sections on slides Message-ID: In a message dated 3/17/2005 9:25:56 AM US Mountain Standard Time, Julie.Sanders@med.va.gov writes: > In the past few days one our pathologists has been complaining about > "uneven > sections." In other words, he's having to focus up and down a lot because > the sections aren't flat on the slide. Julie, It sounds to me like the problem may lie in the microtome. It could be a type of "chatter" in the sections. They may indeed not be flat on the slide, or they could be of uneven thickness. This is almost always because something is loose on the microtome, such as the specimen clamp or something in the knife holder, and the main offender is usually the knife holder. A quick check would be to cut the same block on different microtomes, then ask the doc to evaluate the sections. See if you can pinpoint the problem to a specific instrument. If you find you have a problem with one microtome, be sure to check out the knife holder and the clamping mechanism for the blade. It could be something as simple as paraffin debris in the blade clamping mechanism, or maybe the clamping pressure needs to be adjusted or the mechanism's springs need to be replaced. Just my two cents' worth. Hope it helps! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. From POWELL_SA <@t> Mercer.edu Thu Mar 17 10:54:21 2005 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] uneven sections on slides In-Reply-To: <457381D92B01BD44B21CF37CC02EBDFD029275B3@vhacinexc5.v10.med.va.gov> Message-ID: <01LLZW3HK5BG8WWV13@Macon2.Mercer.edu> In the past when I had this problem, the mounting media was too thick and another time I had gotten a bad batch of coverslips, both of which were easily corrected. Coverslips have better QC now so I doubt if that is the problem. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julie.Sanders@med.va.gov Sent: Thursday, March 17, 2005 11:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] uneven sections on slides Hello All, In the past few days one our pathologists has been complaining about "uneven sections." In other words, he's having to focus up and down a lot because the sections aren't flat on the slide. Any body have any explanations of why this could be happening and any solutions? We haven't been doing anything different, use plus charged slides and paraplast x-tra paraffin. 15 minutes in a 55 degree C oven and automatic stainer. Any comments are greatly appreciated. Julie Julie Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology VA Medical Center Cincinnati, Oh. 45220 Julie A. Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billingconsultants <@t> yahoo.com Thu Mar 17 11:16:37 2005 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Charging Message-ID: <20050317171638.14475.qmail@web54207.mail.yahoo.com> If A,B, C are from one specimen, then you would charge for 2 immunos. If A,B & C are from separate specimens, then you would charge for 6 immunos. Hope this helps. Louri Billing Consultants, LLC www.billingconsultants.net --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From gcallis <@t> montana.edu Thu Mar 17 11:20:11 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] uneven sections on slides In-Reply-To: <457381D92B01BD44B21CF37CC02EBDFD029275B3@vhacinexc5.v10.me d.va.gov> References: <457381D92B01BD44B21CF37CC02EBDFD029275B3@vhacinexc5.v10.med.va.gov> Message-ID: <6.0.0.22.1.20050317101217.01b94e60@gemini.msu.montana.edu> Make sure you have very sharp blades (maybe techs are not changing blades often enough?), a proper waterbath temperature for your given paraffin - Waterbath may be too cool, so increase a few degrees, allow section to flatten on the waterbath - this is usually our biggest problem when you get in a hurry to get things done. When you pick up sections, do it so section comes into contact with Plus charge slide quickly, not the paraffin but the SECTION. A hint given to me by Marcia from Newcomer Supply!! We keep water level very high, at top edge of waterbath to help you do this, much easier than "fishing around for a section in a low pond" of water for the section. Drain plus charge slides well, slides should be vertical so water drains from under the section, then go to oven. Pooled water, even a tiny amount creates havoc. At 09:10 AM 3/17/2005, you wrote: >Hello All, >In the past few days one our pathologists has been complaining about "uneven >sections." In other words, he's having to focus up and down a lot because >the sections aren't flat on the slide. Any body have any explanations of >why this could be happening and any solutions? We haven't been doing >anything different, use plus charged slides and paraplast x-tra paraffin. 15 >minutes in a 55 degree C oven and automatic stainer. >Any comments are greatly appreciated. >Julie >Julie Sanders, BA,HT(ASCP) >Supervisor, Anatomic Pathology >VA Medical Center >Cincinnati, Oh. 45220 > >Julie A. Sanders, BA,HT(ASCP) >Supervisor, Anatomic Pathology >Cincinnati VAMC > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From liz <@t> premierlab.com Thu Mar 17 11:28:32 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] immunos on whole rat corneas Message-ID: <000001c52b16$bdda4380$76d48a80@AMY> Hello everyone Has anyone had any experience in immunostaining whole rat corneas? My biggest concern is permeabilizing the cornea to get sufficient penetration. In the past I have used 0.1% Triton X-100 for 50 micron free floating vibratome sections and that seemed to work quite well for that application. Any suggestions would be appreciated. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From Lynne.Bell <@t> hitchcock.org Thu Mar 17 11:36:22 2005 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] training...again Message-ID: Dakocytomation paid for me to go to Santa Barbara, CA for one week for training on their immunostainer. It was a wonderful course in a beautiful area of the country. The instructors were knowledgeable and I learned a lot about immunoperoxidase staining, as well as the ins and outs of the Autostainer. We have been using their Autostainer for nearly two years and just love it. Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 From marktarango <@t> earthlink.net Thu Mar 17 12:04:25 2005 From: marktarango <@t> earthlink.net (Mark Adam Tarango) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Re: Histonet Digest, Vol 16, Issue 29 Message-ID: <13898626.1111082665335.JavaMail.root@thecount.psp.pas.earthlink.net> Not very many institutions use red. I've never seen a black cassette.... Mark Tarango Date: Thu, 17 Mar 2005 10:06:55 -0600 From: "Hawkins, Hal K." Subject: [Histonet] cassettes To: Message-ID: <8D6F233E2A5D574B929F3944F3316FD002BD88E6@EXCH2K3.utmb.edu> Content-Type: text/plain; charset="us-ascii" We are planning a multicenter study and would like to use histology embedding cassettes that are distinct from all the colors that are normally used at each institution. Does anyone know of a supplier of cassettes of unusual or distinctive colors or custom colors? Thanks. Hal Hawkins, UT Medical Branch, Galveston, TX From miller <@t> coho.net Thu Mar 17 12:06:12 2005 From: miller <@t> coho.net (Diane G. Miller) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] positive controls - adequacy? References: <81a80b730441eeff9e5235625622bf8d@bigpond.net.au> Message-ID: <050b01c52b1c$05929ba0$0200a8c0@desktop> Hi Satoshi, I work in cell culture and we are using magnetic beads to pull out endothelium cells from the buffy coats of 50 ml blood samples. If you need to know the vendor for the beads, please contact me directly, it was pricey. I stained for CD34 and CD31, the CD 31 is positive, but I'm still having problems getting the right antibody for the CD34. There are internal controls that we know should stain. I have a very nice image of the membrane stain on the CD31 if you would like me to send it to you. It also depends on the passage of the cells, whether you are going to get positive staining or not. I have been very happy with the staining I've done with a new polymer kit that's just come out called PolyDetector by Bio SB. It has eliminated any background staining that I've experienced with other kits, especially since I'm working with Baboon blood. I would think you could use cells from the Buffy coat as a control. Just transfer some cells to 4 well chamber slides and feed with appropriate media. Grow to at least 80% confluency. Then fix with either 4% paraformaldehyde or 10% Buffered formalin for no more than 10 minutes, rinse in TBS, store in 70% alcohol until ready to stain. Then proceed with staining. If I can assist you with any protocols please let me know. Best Regards Diane G. Miller, HT(ASCP), QIHC Senior Research Associate OGI, BME, OHSU 503-627-0130 ----- Original Message ----- From: Satoshi Akima To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 17, 2005 4:17 AM Subject: [Histonet] positive controls - adequacy? Dear Listmembers, this is my first post on the list and I was hoping to canvass some opinions. I am doing some research and would like to stain for neutrophils, lymphocytes and macrophages (to see which cell type seems to have a pathogenic role). The question is what is good enough as a positive control, especially when you are using commercial antibodies for which a published body of literature supporting the specificity of the antibody is out there. Would you: A. Just use a blood smear (whole blood) B. Use white cells from the Buffy coat C. Use a column (like the miniMACS system using antibody couples magnetic beads) to separate out lineages D. In the case of mononuclear cells use Lymphoprep to separate out PBMCs E. Culture your PBMCs to get a purer isolate of macrophages Option C is nice but would cause a big time blow out in the budget - all just to get a positive control for IHC. Seems a bit over the top. I'm also in a hurry to get my paper out and time management is an issue (reagents take time to order in from overseas). What do people think? Thanks for your help. Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Mar 17 12:07:02 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] immunos on whole rat corneas In-Reply-To: <000001c52b16$bdda4380$76d48a80@AMY> References: <000001c52b16$bdda4380$76d48a80@AMY> Message-ID: <6.0.0.22.1.20050317110201.01b21a50@gemini.msu.montana.edu> Liz, I am sending an attachment to you privately although for mouse eye IHC. It was elegant and could be used for rat eyes, with some modifications for eye size, if needed. The author was accessible via email also. They did no special permeabilization for cell surface markers but did use saponin permeabilization for their cytokine work for corneas on whole eye PLP fixed paraffin embedded sections. J of General Virology, Stumpf et al, 83(7):1579, 2002 At 10:28 AM 3/17/2005, you wrote: >Hello everyone > >Has anyone had any experience in immunostaining whole rat corneas? My >biggest concern is permeabilizing the cornea to get sufficient >penetration. In the past I have used 0.1% Triton X-100 for 50 micron >free floating vibratome sections and that seemed to work quite well for >that application. Any suggestions would be appreciated. Thanks in >advance. > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Manager >Premier Laboratory, LLC >P.O. Box 18592 >Boulder, Colorado 80308 >Office: (303) 735-5001 >Fax: (303) 735-3540 >liz@premierlab.com >www.premierlab.com > >Ship to Address: >Premier Laboratory >University of Colorado >MCDB, Room A3B40 >Boulder, Colorado 80309 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From lisab <@t> hoho.org Thu Mar 17 12:22:11 2005 From: lisab <@t> hoho.org (Lisa Brenner) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Re: Histonet Digest, Vol 16, Issue 28 Message-ID: RE; Reprocessing of tissue. Heather, We reprocess tissue, I wouldn't say all the time, but if it needs it we do it. It is normally fatty breast tissue. This happens more often when our pathology assistant is off and one of the pathologists is grossing. We run it through the cleaning cycle on the VIP, then place it in the rack with formalin with the rest of the blocks to be processed that day. It has always worked for us and it cuts like butter. You get beautiful sections that the pathologists can make a diagnosis from. It's better all the way around, especially for the patient. Lisa Brenner HTL Holland Hospital Holland, MI ------------------------------------------------------------ Confidentiality Notice The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. <<<<>>>> From mcauliff <@t> umdnj.edu Thu Mar 17 16:06:51 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] history of staining techs In-Reply-To: <4237FC8B.3040208@medecine.unige.ch> References: <4237FC8B.3040208@medecine.unige.ch> Message-ID: <4239FF7B.4060303@umdnj.edu> In addition to the book I mentioned yesterday there is "History of Microtechnique" by Brian Bracegirdle. I think there is a second edition. Geoff Marco Prunotto wrote: > Dear All, > > I'm post-doc in prof. Gabbiani Lab in the Geneva Pathology Dept., > Medicine Faculty. I'm also interested in history of Science. > Have you got any idea of books or articles on history of staining > techs and histological preparation in general? > > thanks a lot > > Marco Prunotto > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From lindas <@t> awesomenet.net Thu Mar 17 13:47:43 2005 From: lindas <@t> awesomenet.net (Linda Davis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] training References: <56E63440ABF1FC4897947D5BA4CFA44F04A9E14E@ATL1VEXC005.usdom004.tco.tc> Message-ID: <003e01c52b2a$2f7b2eb0$880ac942@D6JLZ851> Ventana sends someone to set up the instrument, work with you on your protocols, and will train you. Linda Davis ----- Original Message ----- From: "Nancy Lemke" To: Sent: Thursday, March 17, 2005 8:28 AM Subject: [Histonet] training Histonetters, Can any or all of you tell me which immunostainer companies offer in house training included with the purchase of an instrument? I know that Dako does but do not know if any of the other companies do. Thanks in advance, Nancy Lemke Asterand, Inc. TechOne 440 Burroughs Detroit, MI 48202 This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Anti-Virus. Version: 7.0.300 / Virus Database: 266.7.2 - Release Date: 3/11/2005 -- No virus found in this outgoing message. Checked by AVG Anti-Virus. Version: 7.0.300 / Virus Database: 266.7.2 - Release Date: 3/11/2005 From JosefaNava <@t> texashealth.org Thu Mar 17 13:55:25 2005 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] ANP.22925 ,Phase 1( Independent predictive markers) Message-ID: <2C515C1049EAF5459EFD8C9B929078A419447E@phdex03.txhealth.org> Hello, I just want to know how many of you have started adding on their Pathology reports (on ER, PR, HER2/neu and EGFR), the information regarding the specimen fixation/processing,antibody clone,and detection system used. I I thank you for any information you can give me. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From jkiernan <@t> uwo.ca Thu Mar 17 14:19:30 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] history of staining techs References: <4237FC8B.3040208@medecine.unige.ch> <4239FF7B.4060303@umdnj.edu> Message-ID: <4239E652.D6C041E0@uwo.ca> Brian Bracegirdle also wrote the chapter on history of staining in the 10th (2002) edition of "Conn's Biological Stains" ISBN 1859960995. It's quite a short chapter (5 or 6 pages) but it has 73 references. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Geoff McAuliffe wrote: > > In addition to the book I mentioned yesterday there is "History of > Microtechnique" by Brian Bracegirdle. I think there is a second edition. > > Geoff > > Marco Prunotto wrote: > > > Dear All, > > > > I'm post-doc in prof. Gabbiani Lab in the Geneva Pathology Dept., > > Medicine Faculty. I'm also interested in history of Science. > > Have you got any idea of books or articles on history of staining > > techs and histological preparation in general? > > > > thanks a lot > > > > Marco Prunotto > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** From brett_connolly <@t> merck.com Thu Mar 17 14:55:55 2005 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Cleaved Caspase-3 antibody and thanks Message-ID: All, Is the Cell Signaling cleaved caspase-3 antibody still the best for IHC on FFPE sections? My in-house antibody bit the dust. Also, thanks to those who replied about my cell block question...I passed the info along. Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From ahenn <@t> vaccinex.com Thu Mar 17 15:02:10 2005 From: ahenn <@t> vaccinex.com (Alicia Henn Ph.D.) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Leica LN22 References: <200503171809.j2HI9vP30317@nexserverpro.vaccinex.com> Message-ID: <001c01c52b34$9626b4e0$2c01a8c0@alicia> Hi all, Has anyone used the Leica LN22 liquid nitrogen cooling system for cutting frozens with the Leica 2265 microtome? Would this make a decent cryostat? Alicia Henn, Ph.D. Vaccinex, Inc. From JMacDonald <@t> mtsac.edu Thu Mar 17 15:06:59 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] uneven sections on slides In-Reply-To: Message-ID: Multi levels of focus can also be attributed to sections that are too thick. Jennifer MacDonald RCHIOVETTI@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 03/17/2005 08:54 AM To Julie.Sanders@med.va.gov, Histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] uneven sections on slides In a message dated 3/17/2005 9:25:56 AM US Mountain Standard Time, Julie.Sanders@med.va.gov writes: > In the past few days one our pathologists has been complaining about > "uneven > sections." In other words, he's having to focus up and down a lot because > the sections aren't flat on the slide. Julie, It sounds to me like the problem may lie in the microtome. It could be a type of "chatter" in the sections. They may indeed not be flat on the slide, or they could be of uneven thickness. This is almost always because something is loose on the microtome, such as the specimen clamp or something in the knife holder, and the main offender is usually the knife holder. A quick check would be to cut the same block on different microtomes, then ask the doc to evaluate the sections. See if you can pinpoint the problem to a specific instrument. If you find you have a problem with one microtome, be sure to check out the knife holder and the clamping mechanism for the blade. It could be something as simple as paraffin debris in the blade clamping mechanism, or maybe the clamping pressure needs to be adjusted or the mechanism's springs need to be replaced. Just my two cents' worth. Hope it helps! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Mar 17 15:09:16 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] positive controls - adequacy? In-Reply-To: <81a80b730441eeff9e5235625622bf8d@bigpond.net.au> Message-ID: What type of preparation will the study slides have? The preparation of your controls slides should match that of the material that you are actually testing. Jennife MacDonald Satoshi Akima Sent by: histonet-bounces@lists.utsouthwestern.edu 03/17/2005 04:17 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] positive controls - adequacy? Dear Listmembers, this is my first post on the list and I was hoping to canvass some opinions. I am doing some research and would like to stain for neutrophils, lymphocytes and macrophages (to see which cell type seems to have a pathogenic role). The question is what is good enough as a positive control, especially when you are using commercial antibodies for which a published body of literature supporting the specificity of the antibody is out there. Would you: A. Just use a blood smear (whole blood) B. Use white cells from the Buffy coat C. Use a column (like the miniMACS system using antibody couples magnetic beads) to separate out lineages D. In the case of mononuclear cells use Lymphoprep to separate out PBMCs E. Culture your PBMCs to get a purer isolate of macrophages Option C is nice but would cause a big time blow out in the budget - all just to get a positive control for IHC. Seems a bit over the top. I'm also in a hurry to get my paper out and time management is an issue (reagents take time to order in from overseas). What do people think? Thanks for your help. Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jr <@t> infosight.com Thu Mar 17 15:34:14 2005 From: jr <@t> infosight.com (John Robertson) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Re Colored Cassettes for multi institution study Message-ID: <4F45E97E-972C-11D9-A589-000393CDFFF4@infosight.com> Message-ID: <8D6F233E2A5D574B929F3944F3316FD002BD88E6@EXCH2K3.utmb.edu> Hal- You might consider a cassette bearing a large letter for each institution. A sequentially bar coded set would permit accurate sample logging and tracking. These markings could be put on the side to permit traditional sharpie markings on the face. Alternatively the face could be marked with large letters leaving room for the sharpie. See a picture at http://www.vialabel.com/bigletter.jpg Hope this helps Hal. John From vsailes <@t> nd.edu Thu Mar 17 13:11:05 2005 From: vsailes <@t> nd.edu (vsailes@nd.edu) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Alpha Fetoprotein for mouse tissue Message-ID: <1111086665.4239d649f2c62@webmail.nd.edu> Hi Everyone, Does anyone know of an Alpha Fetoprotein antibody that works on formalin-fixed paraffin embedded mouse tissue. Any help or suggestions are greatly appreciated. Thanks. Valerie Research technician/Histo-tech University of Notre Dame Notre Dame, IN 46556 From Abizar.A.Lakdawalla <@t> appliedbiosystems.com Thu Mar 17 15:52:37 2005 From: Abizar.A.Lakdawalla <@t> appliedbiosystems.com (Abizar A Lakdawalla) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Re Colored Cassettes for multi institution study In-Reply-To: <4F45E97E-972C-11D9-A589-000393CDFFF4@infosight.com> Message-ID: I have John Kiernan's excellent book "Histological & Histochemical Methods" on sale at e-bay if anyone is interested please click on http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=6951494363 Thanks Abizar From Abizar.A.Lakdawalla <@t> appliedbiosystems.com Thu Mar 17 15:53:43 2005 From: Abizar.A.Lakdawalla <@t> appliedbiosystems.com (Abizar A Lakdawalla) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Kiernan's book for sale Message-ID: Sorry, had the wrong subject heading in the previous e-mail! ----- Forwarded by Abizar A Lakdawalla/FOS/PEC on 03/17/2005 01:52 PM ----- Abizar A Lakdawalla/FOS/PEC 03/17/2005 01:52 PM To Histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Re Colored Cassettes for multi institution study I have John Kiernan's excellent book "Histological & Histochemical Methods" on sale at e-bay if anyone is interested please click on http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=6951494363 Thanks Abizar From MadaryJ <@t> MedImmune.com Thu Mar 17 16:10:32 2005 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] multi colored casettes and slides(Merecedes MEdical) Message-ID: <746FDB897740814EA52BDDCB5ED1DDBC1BA453@medimmune4.medimmune.com> Mercedes Mecical has a wide variety of cassettes and slides as well as ome good pricing on lots of histo stuff. From maria <@t> ski.org Thu Mar 17 17:45:33 2005 From: maria <@t> ski.org (Maria Mejia) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] IHC for wholemount retina References: <002001c52a66$b32b0920$a904a8c0@um.edu> Message-ID: <423A169D.5070001@ski.org> Dr. Marcos, the following is what I did some years back on wholemount turle retina. Please, keep this in mind because a number of positive changes have occured in IHC techniques particularly in regard to IHC reagents. **Use agitation in all steps. 1. transport eyecup in cold (4C) Ringer's solution. 2. fix eyecup quickly in freshly made (cold - 4C) 4% paraformaldehyde (PFA) pH 7.4 - 5mins. **this period of time is sufficient for the retina to become fixed and firm enough to handle. Longer fixation time will cause the retina to adhere strongly to the pigment epithelium. 3. remove from fixative and place again in cold (4C) ringer's solution & using fine forceps & scope QUICKLY, but gently tease retina from eyecup & clean retina of choroid pigment. Remove as much of the vitreous & made 3-5 peripheral incisions (not too big) just enough to facilitate retinal flattening & ensure even fixation & IHC staining. **complete vitreous removal is essential for good fixation & IHC labeling. 4. drop whole retina back into cold 4% PFA @ 4C - overnight (this fix time depends on the antigen of interest). 5. wash retina in 0.1M PB pH 7.4 @ 4C - several changes - 1hr. **the retina is now a free-floating thick section, but can also mount on gel-coated slide (I did mine w/ganglion cell side up) or can place the retina FLAT on piece of 0.45um millipore filter for IHC. Method: 1. treat retina in 0.5% to 1% triton/0.1M PB pH 7.4 - 1-2 hours. 2. rinse in 3 changes of 0.1M PB - 10 minutes each. 3. block endogenous peroxidase activity w/0.3% H202/(with or without) methanol - 20mins. **check if tissue is not wrinkled or folded. 4. wash in PBS-T x3 changes - 10 mins each. 5. second block solution using Casein super blocker (several companies make it, e.g., SkyTek, Innovex) at room temp - 20-30 mins. 6. wash in 0.1MPB x3 changes - 10 mins each. 7. primary antibody/PBS-triton/Casein. 8. wash in PBS-Triton/casein - x3 changes - 5 mins each. 9. biotinylated secondary antibody (I used a super sensitive link at 1:50) at RT - 1-2 hours. 10. wash in PBS-T/casein x3 changes - 5 mins each. 11. peroxidase conjugated streptavidin (I used super sensitive at 1:50) at RT - 1-2 hours. **here time will vary from 1-2 hours or longer (if longer time place in 4C) 12. wash in PBS x3 changes - 5mins each. 13. rinse in Tris buffer 50mM pH 8 - 5mins. 14. react wholemounts with chromagen solution 1. nickel ammonium sulfate - 9.7mg DAB - 3.7mg 50mM Tris buffer pH 8 - 25ml microfilter solution and react - 1-2 hours 15. react in chromagen sol. 2 nickel ammonium sulfate - 9.7mg DAB - 3.7mg 50mM Tris buffer pH8 - 25ml 250ul (0.3% H202) microfilter and react. **there are quite a number of chromagen methods to use this is just one of several I used. **check using scope - stop when desired intensity is reached. 16. wash in x4 changes of PB - 10 minutes each. 17. mount whole mounts on double or triple coated gel slides. Air dry at least 4 hours or overnight. **the wholemounts on filter paper are peeled off and flat mounted on gel-coated slides and air-dryed. 18. Coverslip. That's it! regards MariaBartola Mejia neurohistologist Smith-Kettlewell Eye Research Institute San Francisco, CA 94115 Email: maria@ski.org Hernan Aldana Marcos wrote: >Dears >I need to make immunos in the whole retinas. Can anybody have a protocol to do it? > >Or a similar technique in whole embryos. Times, concentrations or any advices to make Immunohistochemistry in thick tissues. > >Thanks in advance > > > > >Dr. Hern?n J. Aldana Marcos >Facultad de Medicina. Universidad de Mor?n >Machado 914. B1708JPD. Buenos Aires. Argentina >e-mail alternativo hernanjavier@yahoo.com >web: http://hjaldanamarcos.bravepages.com >http://histologia.bigthicketdirectory.net/main.html > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mab70 <@t> medschl.cam.ac.uk Fri Mar 18 02:42:22 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] cassettes Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114B4@mius2.medlan.cam.ac.uk> Surgipath does a wide range of colours. I hope this helps. MargaretMargaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Hawkins, Hal K. [mailto:hhawkins@UTMB.EDU] Sent: Thursday, March 17, 2005 4:07 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cassettes We are planning a multicenter study and would like to use histology embedding cassettes that are distinct from all the colors that are normally used at each institution. Does anyone know of a supplier of cassettes of unusual or distinctive colors or custom colors? Thanks. Hal Hawkins, UT Medical Branch, Galveston, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From manuvici <@t> med.unibo.it Fri Mar 18 05:43:21 2005 From: manuvici <@t> med.unibo.it (M.Vici) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] anti-dyskerin Message-ID: <423ACCE9.17534.C2FD51@localhost> Hi all! I'd like to know if anybody tried SantaCruz anti-dyskerin (C-15) sc- 26982 (by IHC or IF) or if you know of any other commercially available anti-dyskerin successfully tested. Thanks! Manuela Vici From Don.Birgerson <@t> leica-microsystems.com Fri Mar 18 07:13:29 2005 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Leica LN22 Message-ID: Hi Dr. Henn, Comparing the LN22 device and a cryostat is like comparing a sport car with a truck ( they both transport a person but....). Give me a call and we can go over the pros and cons; maybe it would fit your application. Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 7:00 4:00 CT "Alicia Henn Ph.D." To: Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] Leica LN22 western.edu 03/17/2005 03:02 PM Hi all, Has anyone used the Leica LN22 liquid nitrogen cooling system for cutting frozens with the Leica 2265 microtome? Would this make a decent cryostat? Alicia Henn, Ph.D. Vaccinex, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From akbitting <@t> geisinger.edu Fri Mar 18 08:43:02 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From JWEEMS <@t> sjha.org Fri Mar 18 08:47:28 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA456D4@sjhaexc02.sjha.org> We do NOT. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Charles.Embrey <@t> carle.com Fri Mar 18 08:52:54 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: Not here Charles Embrey Carle Clinic Association Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Gladney <@t> se.amedd.army.mil Fri Mar 18 08:59:15 2005 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: <4D55B2E997EFAE4DA6081DDE100B83023D2C5E@amedmlsermc133.amed.ds.army.mil> Not here. Diane C. Gladney, HT (ASCP) Supervisor, Histology/Cytology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart Avenue P.O. Box 484 Ft. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Fri Mar 18 09:00:30 2005 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Cleaved Caspase-3 antibody and thanks Message-ID: <5CB39BCA5724F349BCB748675C6CA1A202C7567E@SJMEMXMB02.stjude.sjcrh.local> I could not get the Cleaved Caspase 3 from Cell Signaling to work for me; however I ordered it from Sigma Cat. # C5737 and it works great. Hope this helps. Also we use a lot of research antibodies and I have found that the Cell Signaling antibodies do not work very well and they are very expensive. I was wondering what experience others have had with antibodies from Cell Signaling. Thanks, Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Thursday, March 17, 2005 2:56 PM To: 'HISTONET' (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Cleaved Caspase-3 antibody and thanks All, Is the Cell Signaling cleaved caspase-3 antibody still the best for IHC on FFPE sections? My in-house antibody bit the dust. Also, thanks to those who replied about my cell block question...I passed the info along. Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------ ------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------ ------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Fri Mar 18 09:07:48 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0B9@bhrv-nt-11.bhrv.nwest.nhs.uk> Not here but we are British; only wear gloves at Dinner Parties, white ones. Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: 18 March 2005 14:43 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again[Scanned] I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BlazekL <@t> childrensdayton.org Fri Mar 18 09:11:10 2005 From: BlazekL <@t> childrensdayton.org (Linda Blazek) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: Not here or the last two places I worked. Linda Blazek, HT (ASCP) Department of Pathology Children's Medical Center Dayton, Ohio 45404 (937) 641-3358 fax (937)641-5482 blazekl@childrensdayton.org >>> "Angela Bitting" 03/18/2005 9:43:02 AM >>> I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Fri Mar 18 09:24:43 2005 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: <63B8B599DE283148B92E83C78B32C15D4C9524@cmhexbe2.childrensmemorial.org> Not here, but I don't understand a point of discussion. The GLOVES is your protection and, some times, it doesn't matter is it fixed tissue or not. Never know how good tissue is fixed. You are in your own responsibility: you and your health........... Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Friday, March 18, 2005 8:53 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here Charles Embrey Carle Clinic Association Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. From cforster <@t> umn.edu Fri Mar 18 09:27:07 2005 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] cleavedCaspase 3 Message-ID: <423AF34B.5020507@umn.edu> Biocare Medical has a nice Caspase 3 and so does Promega. I have used them both on human and mouse tissue wih success. Colleen Forster U of MN From histology.bc <@t> shaw.ca Fri Mar 18 09:31:06 2005 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves ... a question Message-ID: <423AF43A.8070709@shaw.ca> From your e-mail I am not sure if you are you waging a battle for or against the wearing of gloves during embedding and cutting? In all the labs I have ever worked in ( and that's quite a few), nobody has ever worn gloves for embedding/cutting. I don't see what the benefit would be. Paul From Kemlo.Rogerson <@t> elht.nhs.uk Fri Mar 18 09:35:21 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0BA@bhrv-nt-11.bhrv.nwest.nhs.uk> Plus they stop you getting gravy on your fingers! Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Margaryan, Naira [mailto:NMargaryan@childrensmemorial.org] Sent: 18 March 2005 15:25 To: Charles.Embrey; Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again[Scanned] Not here, but I don't understand a point of discussion. The GLOVES is your protection and, some times, it doesn't matter is it fixed tissue or not. Never know how good tissue is fixed. You are in your own responsibility: you and your health........... Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Friday, March 18, 2005 8:53 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here Charles Embrey Carle Clinic Association Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Fri Mar 18 09:34:16 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: I think the point is whether it is REQUIRED by a facility or agency. Of course everyone has the option. But remember: if something is not properly processed then you probably should be wearing a mask as well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, March 18, 2005 10:25 AM To: Charles.Embrey; Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here, but I don't understand a point of discussion. The GLOVES is your protection and, some times, it doesn't matter is it fixed tissue or not. Never know how good tissue is fixed. You are in your own responsibility: you and your health........... Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Friday, March 18, 2005 8:53 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here Charles Embrey Carle Clinic Association Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Mar 18 09:44:45 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA456D8@sjhaexc02.sjha.org> Point of discussion is that it's very difficult to learn to do these very hands on tasks with gloves, when you have been doing without for so long. I thought I'd never get used to doing frozens! There's nothing like a good warm thumb to make thyroid cut. Of course, I'm older than dirt and come from the days when we handled fresh and fixed tissue without gloves! We've come a long way - perhaps we should start new techs off in gloves and then they'd never know the difference. With prions running amuck, it would be a good idea. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Margaryan, Naira Sent: Friday, March 18, 2005 10:25 AM To: Charles.Embrey; Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here, but I don't understand a point of discussion. The GLOVES is your protection and, some times, it doesn't matter is it fixed tissue or not. Never know how good tissue is fixed. You are in your own responsibility: you and your health........... Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Friday, March 18, 2005 8:53 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here Charles Embrey Carle Clinic Association Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From RBARNHART <@t> summithealth.org Fri Mar 18 09:45:19 2005 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: We do not wear gloves for embedding or cutting but we do for coverslipping. Becky >>> "Angela Bitting" 3/18/2005 9:43:02 AM >>> I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Mar 18 09:50:46 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue cassettes Re:Colored Cassettes for multi institution study In-Reply-To: <4F45E97E-972C-11D9-A589-000393CDFFF4@infosight.com> References: <4F45E97E-972C-11D9-A589-000393CDFFF4@infosight.com> Message-ID: <6.0.0.22.1.20050318084130.01b533f8@gemini.msu.montana.edu> Caveat: True Sharpie marker, per se, ink is washed off by processing solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost Marker II pen that LOOK like "sharpie" markers but have special ink that stays on plastic cassettes? At 02:34 PM 3/17/2005, you wrote: >Hal- You might consider a cassette bearing a large letter for each >institution. A sequentially bar coded set would permit accurate sample >logging and tracking. These markings could be put on the side to permit >traditional sharpie markings on the face. Alternatively the face could be >marked with large letters leaving room for the sharpie. > >See a picture at >http://www.vialabel.com/bigletter.jpg > >Hope this helps Hal. >John > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From JQB7 <@t> CDC.GOV Fri Mar 18 10:03:33 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again[Scanned] Message-ID: But the heat from my cigarette keeps melting the tips! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, March 18, 2005 10:35 AM To: 'Margaryan, Naira'; Charles.Embrey; Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again[Scanned] Plus they stop you getting gravy on your fingers! Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Margaryan, Naira [mailto:NMargaryan@childrensmemorial.org] Sent: 18 March 2005 15:25 To: Charles.Embrey; Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again[Scanned] Not here, but I don't understand a point of discussion. The GLOVES is your protection and, some times, it doesn't matter is it fixed tissue or not. Never know how good tissue is fixed. You are in your own responsibility: you and your health........... Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Friday, March 18, 2005 8:53 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here Charles Embrey Carle Clinic Association Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Fri Mar 18 10:02:25 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: Prion shouldn't be that big of an issue unless you eat the block. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, March 18, 2005 10:45 AM To: Margaryan, Naira; Charles.Embrey; Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Point of discussion is that it's very difficult to learn to do these very hands on tasks with gloves, when you have been doing without for so long. I thought I'd never get used to doing frozens! There's nothing like a good warm thumb to make thyroid cut. Of course, I'm older than dirt and come from the days when we handled fresh and fixed tissue without gloves! We've come a long way - perhaps we should start new techs off in gloves and then they'd never know the difference. With prions running amuck, it would be a good idea. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Margaryan, Naira Sent: Friday, March 18, 2005 10:25 AM To: Charles.Embrey; Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here, but I don't understand a point of discussion. The GLOVES is your protection and, some times, it doesn't matter is it fixed tissue or not. Never know how good tissue is fixed. You are in your own responsibility: you and your health........... Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Friday, March 18, 2005 8:53 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves....again Not here Charles Embrey Carle Clinic Association Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 18, 2005 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From kgibbon <@t> qltinc.com Fri Mar 18 10:37:25 2005 From: kgibbon <@t> qltinc.com (kgibbon@qltinc.com) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Cleaved Caspase-3 antibody and thanks Message-ID: Hi Charlene, I have used some of the Cell signalling antibodies and like other companies I find that some of their product works really well at 1:1000 and others have to be used at 1:25. I would like to ask if you which of the cell signalling cleaved caspase 3 antibodies you used? thanks Kevin Gibbon QLT Inc. |---------+-----------------------------------------> | | "Henry, Charlene" | | | | | | Sent by: | | | histonet-bounces@lists.utsouth| | | western.edu | | | | | | | | | 03/18/2005 07:00 AM | | | | |---------+-----------------------------------------> >-------------------------------------------------------------------------------------------------------------------------------| | | | To: "Connolly, Brett M" , | | cc: | | Subject: RE: [Histonet] Cleaved Caspase-3 antibody and thanks | >-------------------------------------------------------------------------------------------------------------------------------| I could not get the Cleaved Caspase 3 from Cell Signaling to work for me; however I ordered it from Sigma Cat. # C5737 and it works great. Hope this helps. Also we use a lot of research antibodies and I have found that the Cell Signaling antibodies do not work very well and they are very expensive. I was wondering what experience others have had with antibodies from Cell Signaling. Thanks, Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Thursday, March 17, 2005 2:56 PM To: 'HISTONET' (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Cleaved Caspase-3 antibody and thanks All, Is the Cell Signaling cleaved caspase-3 antibody still the best for IHC on FFPE sections? My in-house antibody bit the dust. Also, thanks to those who replied about my cell block question...I passed the info along. Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------ ------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------ ------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Fri Mar 18 10:39:33 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves....again Message-ID: NOT. Glen Dawson Milwaukee, WI -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, March 18, 2005 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves....again I'm wondering how many hospital Histology labs out there "require" their techs to cut and/or embed with gloves. I'm waging a battle right now and am looking for back-up. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dholmes <@t> anatomy.umsmed.edu Fri Mar 18 10:46:15 2005 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] REmounting sections Message-ID: I have a very special OLD (approx. 10 years) brain slide with BDA reaction that has been 'crashed' by a 1X objective !! Is there a relatively safe way to remove the coverslip and keep the tissue intact to be remounted on a new slide? Ordinarily, I would soak the slide in xylene to remove the coverslip but I am not sure of the integrity of the tissue and whether it can withstand such harsh treatment. Any suggestions? Thanks - you guys have been wonderfully helpful in the past. From BoozerKA <@t> pa1.ah.org Fri Mar 18 10:50:57 2005 From: BoozerKA <@t> pa1.ah.org (Kathleen Boozer) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] REmounting sections Message-ID: I have been told you can put it in the -50 C freezer and it will pop off....I have never personally tried it though..... >>> "Dianne Holmes" 3/18/2005 8:46:15 AM >>> I have a very special OLD (approx. 10 years) brain slide with BDA reaction that has been 'crashed' by a 1X objective !! Is there a relatively safe way to remove the coverslip and keep the tissue intact to be remounted on a new slide? Ordinarily, I would soak the slide in xylene to remove the coverslip but I am not sure of the integrity of the tissue and whether it can withstand such harsh treatment. Any suggestions? Thanks - you guys have been wonderfully helpful in the past. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TillRenee <@t> uams.edu Fri Mar 18 11:01:00 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] frozen tissues Message-ID: Has anyone done regular paraffin embedded IHC with frozen tissues? I am going to need to do PCNA, Tunel, and an Agouti antibody on frozen mouse livers. I have only tried this once before years ago with rat prostate and testis, and those still worked fine. I wasn't sure whether the type of tissue would matter, or the stain being done. They wanted me to check before we start, though I will probably test just a couple first. I could do frozens...but paraffin would be so much easier for me. Renee' Till From akbitting <@t> geisinger.edu Fri Mar 18 11:16:38 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Gloves ... a question Message-ID: Thank you to everyone who replied to my question about wearing gloves during embedding and cutting blocks. I intentionally did not mention which side of the war I was on because I wanted evryone to respond without feeling threatened (Histonet is such a dangerous place)LOL Thank you again. By the way, I'm on the side of those who DO NOT wear gloves..... Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> Paul Bradbury 03/18/05 10:31 AM >>> >From your e-mail I am not sure if you are you waging a battle for or against the wearing of gloves during embedding and cutting? In all the labs I have ever worked in ( and that's quite a few), nobody has ever worn gloves for embedding/cutting. I don't see what the benefit would be. Paul _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Janet.Bonner <@t> FLHOSP.ORG Fri Mar 18 11:37:45 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue cassettes Re:Colored Cassettes for multi institution study Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41E8@fh2k093.fhmis.net> The Sharpie "industrial" survives the processing chemicals but we use the Statmark pen for the slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Friday, March 18, 2005 10:51 AM To: John Robertson; Histonet@lists.utsouthwestern.edu Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue cassettes Re:Colored Cassettes for multi institution study Caveat: True Sharpie marker, per se, ink is washed off by processing solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost Marker II pen that LOOK like "sharpie" markers but have special ink that stays on plastic cassettes? At 02:34 PM 3/17/2005, you wrote: >Hal- You might consider a cassette bearing a large letter for each >institution. A sequentially bar coded set would permit accurate sample >logging and tracking. These markings could be put on the side to permit >traditional sharpie markings on the face. Alternatively the face could be >marked with large letters leaving room for the sharpie. > >See a picture at >http://www.vialabel.com/bigletter.jpg > >Hope this helps Hal. >John > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> tc.umn.edu Fri Mar 18 13:00:30 2005 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue cassettes Re:Colored Cassettes for multi institution study In-Reply-To: <07AB60D5D7B9754EBF56F360F98D083DEB41E8@fh2k093.fhmis.net> References: <07AB60D5D7B9754EBF56F360F98D083DEB41E8@fh2k093.fhmis.net> Message-ID: <6.1.2.0.0.20050318125934.02868eb0@ander093.email.umn.edu> The Sharpie "industrial" did not work in our processor. LuAnn At 11:37 AM 3/18/05, Bonner, Janet wrote: >The Sharpie "industrial" survives the processing chemicals but we use the >Statmark pen for the slides. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle >Callis >Sent: Friday, March 18, 2005 10:51 AM >To: John Robertson; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue >cassettes Re:Colored Cassettes for multi institution study > > >Caveat: True Sharpie marker, per se, ink is washed off by processing >solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost >Marker II pen that LOOK like "sharpie" markers but have special ink that >stays on plastic cassettes? > > At 02:34 PM 3/17/2005, you wrote: > > >Hal- You might consider a cassette bearing a large letter for each > >institution. A sequentially bar coded set would permit accurate sample > >logging and tracking. These markings could be put on the side to permit > >traditional sharpie markings on the face. Alternatively the face could be > >marked with large letters leaving room for the sharpie. > > > >See a picture at > >http://www.vialabel.com/bigletter.jpg > > > >Hope this helps Hal. > >John > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jr <@t> infosight.com Fri Mar 18 13:15:38 2005 From: jr <@t> infosight.com (John Robertson) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Re. Not Too Sharpie ! Message-ID: <1D02357E-97E2-11D9-9DD8-000393CDFFF4@infosight.com> Thanks to you ( and others) Gayle for your timely alert, I only used "Sharpie" as a shorthand for pen marking - I know well that standard Sharpie brand markers don't survive processing. FYI --The markings shown in the referenced picture survive all processing . John John Robertson P.E. Ph.D. jr@infosight.com CEO InfoSight Corporation http://www.infosight.com " We Barcode Difficult Stuff" tm P.O. Box 5000 20700 Rt. 23 Chillicothe , OH 45601 Phone (740) 642 3600 (Eastern time zone) Fax (740) 642 5001 Private Fax. (740) 642 3106 PGP Key 0x8EDD65A2 Somewhere, something incredible is waiting to be known. -Carl Sagan --------------------------------------------- Date: Fri, 18 Mar 2005 08:50:46 -0700 From: Gayle Callis Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue cassettes Re:Colored Cassettes for multi institution study To: John Robertson , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050318084130.01b533f8@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Caveat: True Sharpie marker, per se, ink is washed off by processing solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost Marker II pen that LOOK like "sharpie" markers but have special ink that stays on plastic cassettes? At 02:34 PM 3/17/2005, you wrote: > Hal- You might consider a cassette bearing a large letter for each > institution. A sequentially bar coded set would permit accurate sample > logging and tracking. These markings could be put on the side to permit > traditional sharpie markings on the face. Alternatively the face could > be > marked with large letters leaving room for the sharpie. > > See a picture at > http://www.vialabel.com/bigletter.jpg > > Hope this helps Hal. > John > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet John Robertson P.E. Ph.D. jr@infosight.com CEO InfoSight Corporation http://www.infosight.com " We Barcode Difficult Stuff" tm P.O. Box 5000 20700 Rt. 23 Chillicothe , OH 45601 Phone (740) 642 3600 (Eastern time zone) Fax (740) 642 5001 Private Fax. (740) 642 3106 PGP Key 0x8EDD65A2 Somewhere, something incredible is waiting to be known. -Carl Sagan From kaleid11 <@t> yahoo.com Fri Mar 18 13:16:37 2005 From: kaleid11 <@t> yahoo.com (Adam Perry) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Problems with Gold-immunolabeling and silver enhancement in brain tissue Message-ID: <20050318191637.99496.qmail@web31603.mail.mud.yahoo.com> I'm trying to perform silver enhancement of my gold labeled secondary antibody (1.4nm gold) in an immunocytochemistry (ICC) protocol in rodent brain tissue. I know that the primary antibody and initial steps are working because when I use an avidin-biotinylated peroxidase kit to visualize a biotinylated secondary antibody (as opposed to using the gold conjugated secondary antibody)I get great labeling of my antigen. I've done a dot blot with the gold labeled antibody and I seem to get silver enhancement of particles only in the dot blot with the gold-labeled antibody (as opposed to unlabeled antibodies)- so I'm pretty sure there are gold particles attached to the IgG. Are there any obvious differences that need to be considered when working with a gold-conjugated antibody as opposed to antibodies that are biotinylated or conjugated to enzymes?? Any insights would be greatly appreciated.. Thanks, Adam Perry Department of Physiology and Biophysics University of Illinois Chicago, IL __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bwhitaker <@t> brownpathology.com Fri Mar 18 13:19:12 2005 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] .."Sharpie" marker pens In-Reply-To: <6.1.2.0.0.20050318125934.02868eb0@ander093.email.umn.edu> Message-ID: <000001c52bef$5e662de0$3601a8c0@brownpathology.net> It didn't work for me either! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Friday, March 18, 2005 1:01 PM To: Bonner, Janet; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Caveat about true "Sharpie" marker pens fortissue cassettes Re:Colored Cassettes for multi institution study The Sharpie "industrial" did not work in our processor. LuAnn At 11:37 AM 3/18/05, Bonner, Janet wrote: >The Sharpie "industrial" survives the processing chemicals but we use >the Statmark pen for the slides. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle >Callis >Sent: Friday, March 18, 2005 10:51 AM >To: John Robertson; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue >cassettes Re:Colored Cassettes for multi institution study > > >Caveat: True Sharpie marker, per se, ink is washed off by processing >solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost >Marker II pen that LOOK like "sharpie" markers but have special ink >that stays on plastic cassettes? > > At 02:34 PM 3/17/2005, you wrote: > > >Hal- You might consider a cassette bearing a large letter for each > >institution. A sequentially bar coded set would permit accurate > >sample logging and tracking. These markings could be put on the side > >to permit traditional sharpie markings on the face. Alternatively the > >face could be marked with large letters leaving room for the sharpie. > > > >See a picture at > >http://www.vialabel.com/bigletter.jpg > > > >Hope this helps Hal. > >John > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lowman034 <@t> yahoo.com Fri Mar 18 13:55:03 2005 From: lowman034 <@t> yahoo.com (Dave Low) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Thin-Prep In-Reply-To: 6667 Message-ID: <20050318195503.24482.qmail@web40914.mail.yahoo.com> Stephen, Yes, you can use the ThinPrep vial solution with your Non-Gyns. Here is the contact number: Cytyc Corporation Customer Service 85 Swanson Road Boxborough, MA 01719 1-800-442-9892 option 5 Good Luck! Dave Low Malcolm Grow Med Ctr --- "Scholz, Stephen J." wrote: > Hello all; > > I was instructed by a Pathologist that I work for to > investigate using the ThinPrep technology in a > non-gyne capacity. Is this done? I know that it is > used for gyne specimens but I have never heard of > using the technology for non-gyne. I'm a Histo-tech > and my experience with cytology is limited so any > information that I could get would be appreciated. > (where to get the instrument, cost, is it > applicable) > > Thank you, > > Stephen J. Scholz HT(ASCP) > > 815-395-5410 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From pzeitlow <@t> bbpllab.com Fri Mar 18 14:06:10 2005 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Ventana Benchmark HPV Message-ID: <813FB33DA405334F947F8BFC6EBD0B2AE69E@bbplsrv1.bbpl> Has anyone doing HPV ISH on Benchmark (not XT) experienced background problems on slides that prohibits evaluation? We are seeing random problems with this "Blue haze" and cannot seem to get it resolved. I realize I haven't provided much detail, but if anyone is having this same issue, they will recognize my description. Thanks in advance for any input! Pat Z Department of Molecular Pathology Boyce and Bynum Pathology Labs, P.C. From gcallis <@t> montana.edu Fri Mar 18 14:42:52 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Industrial Sharpie good news In-Reply-To: <07AB60D5D7B9754EBF56F360F98D083DEB41E8@fh2k093.fhmis.net> References: <07AB60D5D7B9754EBF56F360F98D083DEB41E8@fh2k093.fhmis.net> Message-ID: <6.0.0.22.1.20050318133223.01b22008@gemini.msu.montana.edu> Dear Janet, Thank you for info about Industrial Sharpie ink staying on in solvents. Where are you purchasing these special Sharpies? Staples, or some other vendor? I was worried that someone inadvertently grabs a run of the mill, everyday Sharpie marker used for labeling bottles, tape, and label their tissue cassettes or slides! We have had this happen with disasterous results when a whole project's labeling is dissolved away because the unaware used the wrong Sharpie marker. I plan to give the industrial Sharpies a try though. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Fri Mar 18 14:52:44 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Industrial pen woes! Oh Dear! In-Reply-To: <6.1.2.0.0.20050318125934.02868eb0@ander093.email.umn.edu> References: <07AB60D5D7B9754EBF56F360F98D083DEB41E8@fh2k093.fhmis.net> <6.1.2.0.0.20050318125934.02868eb0@ander093.email.umn.edu> Message-ID: <6.0.0.22.1.20050318135135.01b47008@gemini.msu.montana.edu> Hmmm guess I won't take the chance with industrial pens after all!!! Gayle Callis At 12:00 PM 3/18/2005, you wrote: >The Sharpie "industrial" did not work in our processor. >LuAnn > >At 11:37 AM 3/18/05, Bonner, Janet wrote: >>The Sharpie "industrial" survives the processing chemicals but we use the >>Statmark pen for the slides. >> >>- From gcallis <@t> montana.edu Fri Mar 18 15:08:05 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Problems with Gold-immunolabeling and silver enhancement in brain tissue In-Reply-To: <20050318191637.99496.qmail@web31603.mail.mud.yahoo.com> References: <20050318191637.99496.qmail@web31603.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20050318135726.01b24978@gemini.msu.montana.edu> How are you doing the silver enhancement, from inhouse prepared reagents? We found making up enhancement reagents inhouse a lot of work, then it was hard to control the enhancement, a painstaking procedure. Dr. Chris van der Loos uses a silver enhancement kit from http://www.aurion.nl . When I visited his lab, this was a snap to use the Aurion kit and we had excellent results. For visualizing IGGS staining, epi illumination is superior than trying to find the black enhanced particles via standard transmitted light microscopy. You may have staining, but can't see it very well - however with epi-illumination, the enhanced gold particles show up like little light bulbs!! You can also ask him about his immunogold methods at . At 12:16 PM 3/18/2005, you wrote: >I'm trying to perform silver enhancement of my gold >labeled secondary antibody (1.4nm gold) in an >immunocytochemistry (ICC) protocol in rodent brain >tissue. I know that the primary antibody and initial >steps are working because when I use an >avidin-biotinylated peroxidase kit to visualize a >biotinylated secondary antibody (as opposed to using >the gold conjugated secondary antibody)I get great >labeling of my antigen. I've done a dot blot with the >gold labeled antibody and I seem to get silver >enhancement of particles only in the dot blot with the >gold-labeled antibody (as opposed to unlabeled >antibodies)- so I'm pretty sure there are gold >particles attached to the IgG. > >Are there any obvious differences that need to be >considered when working with a gold-conjugated >antibody as opposed to antibodies that are >biotinylated or conjugated to enzymes?? > >Any insights would be greatly appreciated.. >Thanks, >Adam Perry >Department of Physiology and Biophysics >University of Illinois >Chicago, IL > >__________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Fri Mar 18 15:13:39 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:47 2005 Subject: [Histonet] Not Too Sharpie ! In-Reply-To: <1D02357E-97E2-11D9-9DD8-000393CDFFF4@infosight.com> References: <1D02357E-97E2-11D9-9DD8-000393CDFFF4@infosight.com> Message-ID: <6.0.0.22.1.20050318140841.01b497e0@gemini.msu.montana.edu> Dear John, I have to thank others too plus I learned about industrial Sharpie markers, new to me! Better alerted than dead in the sea of solvents we deal with. I appeciate it when others give me heads up warnings too - saves me a lot of grief. Gayle Callis At 12:15 PM 3/18/2005, you wrote: >Thanks to you ( and others) Gayle for your timely alert, > >I only used "Sharpie" as a shorthand for pen marking - I know well that >standard Sharpie brand markers don't survive processing. FYI --The >markings shown in the referenced picture survive all processing . > John > >John Robertson P.E. Ph.D. jr@infosight.com >CEO InfoSight Corporation http://www.infosight.com " We >Barcode Difficult Stuff" tm >P.O. Box 5000 >20700 Rt. 23 Chillicothe , OH 45601 >Phone (740) 642 3600 (Eastern time zone) >Fax (740) 642 5001 >Private Fax. (740) 642 3106 >PGP Key 0x8EDD65A2 From kaleid11 <@t> yahoo.com Fri Mar 18 15:44:49 2005 From: kaleid11 <@t> yahoo.com (Adam Perry) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re: Problems with Gold-immunolabeling and silver enhancement in brain tissue In-Reply-To: 6667 Message-ID: <20050318214449.2414.qmail@web31604.mail.mud.yahoo.com> I actually started with in house prepared reagents...tried every protocol I could get my hands on and none of them really worked well and all were time consuming to prepare...so I broke down and bought a kit (from Aurion) and it was much easier to use, but didn't seem to work. I haven't tried the epi-illumination scope yet though. What did your tissue sections look like after IGGS? In the original lab I trained in, the sections would go through a color change from yellow, to brown, to greenish brown and then we'd stop the silver enhancement. The antigen I'm staining for is CREB (transcription factor found all throughout the brain), so that may be why the entire section went through the color change...but the staining I did with the Aurion kit...the tissue looks amazingly clean, no background staining...or anything...is that typical with IGGS? Thanks again, Adam Department of Physiology and Biophysics University of Illinois Chicago, IL --- Gayle Callis wrote: > How are you doing the silver enhancement, from > inhouse prepared > reagents? We found making up enhancement reagents > inhouse a lot of work, > then it was hard to control the enhancement, a > painstaking procedure. > > Dr. Chris van der Loos uses a silver enhancement kit > from > http://www.aurion.nl . When I visited his lab, this > was a snap to use the > Aurion kit and we had excellent results. For > visualizing IGGS staining, > epi illumination is superior than trying to find the > black enhanced > particles via standard transmitted light microscopy. > You may have > staining, but can't see it very well - however with > epi-illumination, the > enhanced gold particles show up like little light > bulbs!! > > You can also ask him about his immunogold methods at > > . > > At 12:16 PM 3/18/2005, you wrote: > >I'm trying to perform silver enhancement of my gold > >labeled secondary antibody (1.4nm gold) in an > >immunocytochemistry (ICC) protocol in rodent brain > >tissue. I know that the primary antibody and > initial > >steps are working because when I use an > >avidin-biotinylated peroxidase kit to visualize a > >biotinylated secondary antibody (as opposed to > using > >the gold conjugated secondary antibody)I get great > >labeling of my antigen. I've done a dot blot with > the > >gold labeled antibody and I seem to get silver > >enhancement of particles only in the dot blot with > the > >gold-labeled antibody (as opposed to unlabeled > >antibodies)- so I'm pretty sure there are gold > >particles attached to the IgG. > > > >Are there any obvious differences that need to be > >considered when working with a gold-conjugated > >antibody as opposed to antibodies that are > >biotinylated or conjugated to enzymes?? > > > >Any insights would be greatly appreciated.. > >Thanks, > >Adam Perry > >Department of Physiology and Biophysics > >University of Illinois > >Chicago, IL > > > >__________________________________________________ > >Do You Yahoo!? > >Tired of spam? Yahoo! Mail has the best spam > protection around > >http://mail.yahoo.com > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > __________________________________ Do you Yahoo!? Make Yahoo! your home page http://www.yahoo.com/r/hs From timothy.macatee <@t> med.nyu.edu Fri Mar 18 15:33:03 2005 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Mouse histology books Message-ID: Hello everyone, Long time reader, first time writer. Since there has been a discussion about books, I was wondering if there is a good adult mouse histology book out there. This way I can see what things are 'suppose' to look like, and then I can test my abilities to orient and embed and cut and stain and ... Thanks Tim Macatee NYU Medical Center -- From Abizar.A.Lakdawalla <@t> appliedbiosystems.com Fri Mar 18 16:44:03 2005 From: Abizar.A.Lakdawalla <@t> appliedbiosystems.com (Abizar A Lakdawalla) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Mouse histology books In-Reply-To: Message-ID: Checkout this online atlas. http://www.deltagen.com/target/histologyatlas/HistologyAtlas.html Abizar Timothy Macatee Sent by: histonet-bounces@lists.utsouthwestern.edu 03/18/2005 01:33 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Mouse histology books Hello everyone, Long time reader, first time writer. Since there has been a discussion about books, I was wondering if there is a good adult mouse histology book out there. This way I can see what things are 'suppose' to look like, and then I can test my abilities to orient and embed and cut and stain and ... Thanks Tim Macatee NYU Medical Center -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lubbockcat <@t> hotmail.com Fri Mar 18 20:50:24 2005 From: lubbockcat <@t> hotmail.com (Terry Murphy) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Gloves ... a question In-Reply-To: Message-ID: I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? Terry Murphy HTL(ASCP) >From: "Angela Bitting" >To: , >Subject: Re: [Histonet] Gloves ... a question >Date: Fri, 18 Mar 2005 12:16:38 -0500 > >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. >I intentionally did not mention which side of the war I was on because I >wanted evryone to respond without feeling threatened (Histonet is such a >dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > >>> Paul Bradbury 03/18/05 10:31 AM >>> > >From your e-mail I am not sure if you are you waging a battle for or >against the wearing of gloves during embedding and cutting? > >In all the labs I have ever worked in ( and that's quite a few), nobody >has ever worn gloves for embedding/cutting. I don't see what the benefit > >would be. > >Paul > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >IMPORTANT WARNING: The information in this message (and the documents >attached to it, if any) is confidential and may be legally privileged. It >is intended solely for the addressee. Access to this message by anyone else >is unauthorized. If you are not the intended recipient, any disclosure, >copying, distribution or any action taken, or omitted to be taken, in >reliance on it is prohibited and may be unlawful. If you have received this >message in error, please delete all electronic copies of this message (and >the documents attached to it, if any), destroy any hard copies you may have >created and notify me immediately by replying to this email. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ On the road to retirement? Check out MSN Life Events for advice on how to get there! http://lifeevents.msn.com/category.aspx?cid=Retirement From cfavara <@t> niaid.nih.gov Sat Mar 19 09:23:00 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Gloves ... a question Message-ID: I have seen this myself. I think most pathologists are skilled at tuning out such things. I wear glove for this reason as well as many others. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Terry Murphy [mailto:lubbockcat@hotmail.com] Sent: Friday, March 18, 2005 7:50 PM To: akbitting@geisinger.edu; histonet@pathology.swmed.edu; histology.bc@shaw.ca Subject: Re: [Histonet] Gloves ... a question I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? Terry Murphy HTL(ASCP) >From: "Angela Bitting" >To: , >Subject: Re: [Histonet] Gloves ... a question >Date: Fri, 18 Mar 2005 12:16:38 -0500 > >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. >I intentionally did not mention which side of the war I was on because I >wanted evryone to respond without feeling threatened (Histonet is such a >dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > >>> Paul Bradbury 03/18/05 10:31 AM >>> > >From your e-mail I am not sure if you are you waging a battle for or >against the wearing of gloves during embedding and cutting? > >In all the labs I have ever worked in ( and that's quite a few), nobody >has ever worn gloves for embedding/cutting. I don't see what the benefit > >would be. > >Paul > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >IMPORTANT WARNING: The information in this message (and the documents >attached to it, if any) is confidential and may be legally privileged. It >is intended solely for the addressee. Access to this message by anyone else >is unauthorized. If you are not the intended recipient, any disclosure, >copying, distribution or any action taken, or omitted to be taken, in >reliance on it is prohibited and may be unlawful. If you have received this >message in error, please delete all electronic copies of this message (and >the documents attached to it, if any), destroy any hard copies you may have >created and notify me immediately by replying to this email. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ On the road to retirement? Check out MSN Life Events for advice on how to get there! http://lifeevents.msn.com/category.aspx?cid=Retirement _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janick.jean-marie <@t> laposte.net Sat Mar 19 15:18:09 2005 From: janick.jean-marie <@t> laposte.net (janick.jean-marie) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] lipids drpos inclusion in IHC for frozen sections Message-ID: Hi fellows !!! I have a prolem with IHC on frozen section ... I can see lipid inclusion (lipids drops)... in my section when i look my immunofluorescence I use this protocol ; 1. Formald?hyde ? 4% for 15 min 2. Bath of PBS 1x for 15 min 3. Antiboby A? against Beta-GAl(1/500) and against SCA-1(1/300) 4. 1 hour in humid chamber 5. Bath of PBS 1x for 15 min 6. 2 flurorescent secondary antibody (1/300) (Rhodamin and Fluorescein) 7. humid chamber for 30 min 8. wash with PBS 9. add mowiol for mountig scale 10. Stock at -80?C Have you got an idea for explain my problem ... and what can i do for remove the lipids drops ? Acc?dez au courrier ?lectronique de La Poste : www.laposte.net ; 3615 LAPOSTENET (0,34?/mn) ; t?l : 08 92 68 13 50 (0,34?/mn) From janick.jean-marie <@t> laposte.net Sat Mar 19 15:39:04 2005 From: janick.jean-marie <@t> laposte.net (janick.jean-marie) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] [histonet ] inclusion of lipids drops in ICH for frozen sections Message-ID: Hi fellows !!! I have a prolem with IHC on frozen section ... I can see lipid inclusion (lipids drops)... in my section when i look my immunofluorescence I use this protocol ; 1. Formald?hyde ? 4% for 15 min 2. Bath of PBS 1x for 15 min 3. Antiboby A? against Beta-GAl(1/500) and against SCA-1(1/300) 4. 1 hour in humid chamber 5. Bath of PBS 1x for 15 min 6. 2 flurorescent secondary antibody (1/300) (Rhodamin and Fluorescein) 7. humid chamber for 30 min 8. wash with PBS 9. add mowiol for mountig scale 10. Stock at -80?C Have you got an idea for explain my problem ... and what can i do for remove the lipids drops ? THANKS !!! Janick JEAN-MARIE CRLC INSERM Acc?dez au courrier ?lectronique de La Poste : www.laposte.net ; 3615 LAPOSTENET (0,34?/mn) ; t?l : 08 92 68 13 50 (0,34?/mn) From leswes <@t> shaw.ca Sat Mar 19 15:56:13 2005 From: leswes <@t> shaw.ca (Lesley Weston) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Gloves....again In-Reply-To: Message-ID: There's also the point that if it is not required, then someone who chooses to play it safe by wearing gloves might have to pay for them themselves, while if it is required, they wouldn't have to. -- Lesley Weston > From: "Bartlett, Jeanine" > Date: Fri, 18 Mar 2005 10:34:16 -0500 > To: "Margaryan, Naira" , "Charles.Embrey" > , Angela Bitting , > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Gloves....again > > I think the point is whether it is REQUIRED by a facility or agency. Of > course everyone has the option. But remember: if something is not > properly processed then you probably should be wearing a mask as well. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Margaryan, Naira > Sent: Friday, March 18, 2005 10:25 AM > To: Charles.Embrey; Angela Bitting; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Gloves....again > > > Not here, but I don't understand a point of discussion. The GLOVES is > your protection and, some times, it doesn't matter is it fixed tissue or > not. Never know how good tissue is fixed. You are in your own > responsibility: you and your health........... > > Naira V. Margaryan, Ph.D, D.V.M. > Research Associate II > Children's Memorial Research Center > 2300 Children's Plaza, Box 222 > Chicago, IL 60614-3394 > Tel: 773-880-4000/5-6740 > Fax: 773-755-6594 > nmargaryan@childrensmemorial.org > > For Express Mail: > CMRC, Room C.473 > 2430 N. Halsted Street > Chicago, IL 60614-4314 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Charles.Embrey > Sent: Friday, March 18, 2005 8:53 AM > To: Angela Bitting; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Gloves....again > > Not here > Charles Embrey > Carle Clinic Association > Urbana, IL > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela > Bitting > Sent: Friday, March 18, 2005 8:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Gloves....again > > I'm wondering how many hospital Histology labs out there "require" their > techs to cut and/or embed with gloves. I'm waging a battle right now > and am looking for back-up. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. > It is intended solely for the addressee. Access to this message by > anyone else is unauthorized. If you are not the intended recipient, any > disclosure, copying, distribution or any action taken, or omitted to be > taken, in reliance on it is prohibited and may be unlawful. If you have > received this message in error, please delete all electronic copies of > this message (and the documents attached to it, if any), destroy any > hard copies you may have created and notify me immediately by replying > to this email. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The contents of this e-mail message and any attachments are private and > confidential communications intended solely for the addressee(s) named > in this message. If you are not the intended recipient of this message, > please 1) immediately notify the sender by reply e-mail and then delete > this message and its attachments and 2) do not read, use, distribute > disclose or copy this message and/or any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janick.jean-marie <@t> laposte.net Sat Mar 19 15:58:17 2005 From: janick.jean-marie <@t> laposte.net (janick.jean-marie) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] probelm with an immunofluorescence of mammary gland on paraffin sections Message-ID: Hi fellows !! I have a problem with an immunofluorescence of mammary gland on paraffin sections Indeed , I have not a specific immunoflurescence marking ... My protocol : Deparaffinization stage : ? Xyl?ne , three times for min each ? 100% EtOH twice for 5 min ? 95% EtOH once for 5 min ? diH2O or PBS 1X Antigen Retrieval stage : Citrate buffer (10mM) Reagents: a. Citric Acid, monohydrate, granular Storage: Room Temperature b. Filtered distilled water c. 10M Sodium Hydroxide Preparation: 10 mM Citrate Buffer a. Dissolve 0.525g citric acid monohydrate in 250mL of distilled H20 (dH2O) b. pH to 6.0 +/- 0.3 with 10M NaOH Storage: Room Temperature -Preheated container water at 94?C and place the container of Citrate buffer in the bath. -Put the slides in the citrate buffer for 45 min -Remove the Citrate Buffer container with the slides , out of the bath and let?s cooling at RT for 20 min -Plunge the slide in PBS (or H2O) for stop the reaction ? Begin the Immunofluorescence Protocol 1. Antiboby A? against Beta-GAl(1/500) 2. 1 hour in humid chamber 3. Bath of PBS 1x for 15 min 4. flurorescent secondary antibody (1/300) (Rhodamin) 5. humid chamber for 30 min 6. wash with PBS 7. add mowiol for mountig scale 8. Stock at -80?C .... I don't knows what's wrong .... maybe i can use a wash of PBS-tween and before the step with Antibody A? , i can make a bath of milk(2%) for 1 hour ... Wath do you mean about my protocol and suggestions ? Another idea ? THANKS !!! Janick JEAN-MARIE CRLC INSERM Acc?dez au courrier ?lectronique de La Poste : www.laposte.net ; 3615 LAPOSTENET (0,34?/mn) ; t?l : 08 92 68 13 50 (0,34?/mn) From janick.jean-marie <@t> laposte.net Sat Mar 19 16:06:26 2005 From: janick.jean-marie <@t> laposte.net (janick.jean-marie) Date: Fri Sep 16 15:24:48 2005 Subject: [histonet] inclusion of lipids drops in Imunoluorecsece of frozen section Message-ID: Hi fellows !!! I have a prolem with an immunofluorescence on frozen section of mammary gland ... I can see lipid inclusion (lipids drops)... in my section when i look my immunofluorescence I use this protocol ; 1. Formalin 4% for 15 min 2. Bath of PBS 1x for 15 min 3. Antiboby A? against Beta-GAl(1/500) and against SCA-1(1/300) 4. 1 hour in humid chamber 5. Bath of PBS 1x for 15 min 6. 2 flurorescent secondary antibody (1/300) (Rhodamin and Fluorescein) 7. humid chamber for 30 min 8. wash with PBS 9. add mowiol for mountig scale 10. Stock at -80?C Have you got an idea for explain my problem ... and what can i do for remove the lipids drops ? THANKS !!! Janick JEAN-MARIE CRLC INSERM Acc?dez au courrier ?lectronique de La Poste : www.laposte.net ; 3615 LAPOSTENET (0,34?/mn) ; t?l : 08 92 68 13 50 (0,34?/mn) From RSRICHMOND <@t> aol.com Sat Mar 19 18:27:52 2005 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Gloves ... a question Message-ID: <46.6595c27a.2f6e1d88@aol.com> Terry Murphy HTL(ASCP) notes: >>I once had a pathologist complain [about] dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist?<< and Cynthia Favara, NIAID/NIH/RML/LPVD, in Hamilton, Montana replies: >>I have seen this myself. I think most pathologists are skilled at tuning out such things. I wear glove for this reason as well as many others.<< This old pathologist has ignored a lot of skin cells on slides - occasionally they're a nuisance in a confusing cytologic preparation, or in a cytokeratin immunostain - but normally they never rise to the level of consciousness. My present hospital is trying to be latex-free. Nitrile rubber gloves (the purple ones) are a lot more resistant to formaldehyde than latex gloves are. Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From lpwenk <@t> sbcglobal.net Sat Mar 19 18:39:08 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Are Gloves a CAP regulation? References: <807FE48C5A7CC940B973B58D32E7014318F9332A@nhpens-exch1.pcola.med.navy.mil> Message-ID: <016301c52ce5$3b0a7080$9cf7ff44@domainnotset.invalid> Long explanation, so if not interested in what CAP and OSHA say about gloves, BBP and fixed/processed tissues, delete immediately. Everything copied directly from a website is in quotes. My comments are not. I went to the CAP Laboratory General checklist - www.cap.org "GEN.72550 Phase II N/A YES NO Is appropriate personal protective equipment (gloves, gowns, masks and eye protectors, etc.) provided and maintained in a sanitary and reliable condition in all technical work areas in which blood and body substances are handled and in circumstances during which exposure is likely to occur? NOTE: If respiratory protection is needed because of potential exposure to an infectious agent by aerosol or droplet, personnel should use either a properly fit-tested NIOSH-approved filter respirator (N-95 or higher) or a powered air-purifying respirator (PAPRS) equipped with high efficiency particulate air (HEPA) filters. Accurate fit testing is a key component of effective respirator use. COMMENTARY: N/A REFERENCES: 1) Centers for Disease Control. Guidelines for prevention of transmission of human immunodeficiency virus and hepatitis B virus to health-care and public-safety workers. MMWR. 1989:38(suppl S-6):1-37; 2) Krienitz DR. Safety education in the laboratory. Lab Med. 1996;27:823-827; 3) Occupational Safety and Health Administration. Toxic and hazardous substances. Bloodborne pathogens. Washington, DC: US Government Printing Office, 1999(Jul 1): [29CFR1910.1030]; 4) Prinz Luebbert P. Q&A. Wearing laboratory coats during break. Lab Med. 1999;30:710; 5) NCCLS. Protection of laboratory workers from occupationally acquired infections; approved guideline M29-A2. Wayne, PA: NCCLS, 2002. " Notice it refers to OSHA 29CFR1910.1030 - Bloodborne pathogens. So I went to the OSHA website http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id =10051 "Occupational Exposure means reasonably anticipated skin, eye, mucous membrane, or parenteral contact with blood or other potentially infectious materials that may result from the performance of an employee's duties." "Other Potentially Infectious Materials means (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body fluids in situations where it is difficult or impossible to differentiate between body fluids; (2) Any unfixed tissue or organ (other than intact skin) from a human (living or dead); and (3) HIV-containing cell or tissue cultures, organ cultures, and HIV- or HBV-containing culture medium or other solutions; and blood, organs, or other tissues from experimental animals infected with HIV or HBV." "Parenteral means piercing mucous membranes or the skin barrier through such events as needlesticks, human bites, cuts, and abrasions." "Personal Protective Equipment is specialized clothing or equipment worn by an employee for protection against a hazard. General work clothes (e.g., uniforms, pants, shirts or blouses) not intended to function as protection against a hazard are not considered to be personal protective equipment." So - my interpretation = infectious material that pertains to Histology would be #2 - UNFIXED tissues or organs. So gloves should be worn when using a cryostat, working with fresh unfixed tissue, for example. Continuing - "General. Universal precautions shall be observed to prevent contact with blood or other potentially infectious materials. Under circumstances in which differentiation between body fluid types is difficult or impossible, all body fluids shall be considered potentially infectious materials." Also - "Engineering and work practice controls shall be used to eliminate or minimize employee exposure. Where occupational exposure remains after institution of these controls, personal protective equipment shall also be used." Concerning PPEs - "Provision. When there is occupational exposure, the employer shall provide, at no cost to the employee, appropriate personal protective equipment such as, but not limited to, gloves, gowns, laboratory coats, face shields or masks and eye protection, and mouthpieces, resuscitation bags, pocket masks, or other ventilation devices. Personal protective equipment will be considered "appropriate" only if it does not permit blood or other potentially infectious materials to pass through to or reach the employee's work clothes, street clothes, undergarments, skin, eyes, mouth, or other mucous membranes under normal conditions of use and for the duration of time which the protective equipment will be used." "Gloves. Gloves shall be worn when it can be reasonably anticipated that the employee may have hand contact with blood, other potentially infectious materials, mucous membranes, and non-intact skin; when performing vascular access procedures except as specified in paragraph (d)(3)(ix)(D); and when handling or touching contaminated items or surfaces." My interpretation - Universal precautions (which includes using gloves) are necessary when there is the potential for exposure to infectious material. If tissue is well fixed and processed, there is no need for gloves. In the case of something like prions, which are not inactivated with fixation/processing, then gloves might be a good idea when embedding/sectioning brain (though as someone else pointed out, you probably can only catch it if you eat the fixed/processed brain tissue, not from touching it). If you push the tissue flat in the mold with the forceps (i.e., you are not touching the tissue), there is no need for gloves. People can be taught to not touch the tissue (engineering controls). Some of my students wear gloves while embedding, some don't. (I tell them they don't have to wear them, that it is not a regulation, fixation kills microorganisms, that there is no documented exposure from embedding.) I don't wear gloves, so they aren't learning it from me. But some of our histotechs do, some don't. Those students and techs who do wear gloves fall into one of three categories of reasons: 1) they don't like the feel of the paraffin on their fingers, especially when it starts to solidify, 2) their fingers are sensitive to the temperature of the paraffin and they don't want to wait until they develop the histotech's ability to handle 60 degree C. paraffin, or 3) they still have a fear of "catching something", even after lectures on fixation and processing. Some Histonetter did raise a point that, since it is not an OSHA requirement, does the institution have to pay for the gloves? OSHA does say the employer must provide free gloves if there is a risk on occupational exposure. OSHA doesn't say that the employer has to provide PPE if it is not a requirement. That would be up to the employer as to whether they want to pay for them or not. But, in my opinion, if we are talking about 1 pair of gloves a day for a histotech to embed, and they are a good employee that you don't want to lose, and it makes them feel "safer", and by giving them the gloves, they will continue to work for you, I think the employer can afford the gloves. Cheaper than having to find a new histotech, or train a new histotech. (In my humble opinion.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Wednesday, March 16, 2005 8:10 AM Subject: [Histonet] Are Gloves a CAP regulation? > Since I work at a military facility, I have notice that all the military > histo techs wear gloves to embed and cut. I went to school and we never did > this. When CAP came last year, my co-worker insisted that this was a CAP > regulation. Does anybody know if this is a CAP regulation, because this is > news to me and I was never taught in school to wear gloves to embed or to > cut. Thank you. > > > > Heather A. Harper > > Supervisor of Histology/Morgue > > Naval Hospital of Pensacola, FL > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Mon Mar 21 03:01:51 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Thin-Prep[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0BB@bhrv-nt-11.bhrv.nwest.nhs.uk> I don't think you use the ThinPrep vial, I believe you use the one that is a preservative and lyses red blood cells, is it Cytolyse? It's in the manual. If you use the ThinPrep vial then you have problems with rbc's which become fixed. -----Original Message----- From: Dave Low [mailto:lowman034@yahoo.com] Sent: 18 March 2005 19:55 To: Scholz, Stephen J.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Thin-Prep[Scanned] Stephen, Yes, you can use the ThinPrep vial solution with your Non-Gyns. Here is the contact number: Cytyc Corporation Customer Service 85 Swanson Road Boxborough, MA 01719 1-800-442-9892 option 5 Good Luck! Dave Low Malcolm Grow Med Ctr --- "Scholz, Stephen J." wrote: > Hello all; > > I was instructed by a Pathologist that I work for to > investigate using the ThinPrep technology in a > non-gyne capacity. Is this done? I know that it is > used for gyne specimens but I have never heard of > using the technology for non-gyne. I'm a Histo-tech > and my experience with cytology is limited so any > information that I could get would be appreciated. > (where to get the instrument, cost, is it > applicable) > > Thank you, > > Stephen J. Scholz HT(ASCP) > > 815-395-5410 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Mon Mar 21 07:48:12 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Thin-Prep[Scanned] Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D21C@sjhaexc02.sjha.org> There are non-gyn supplies - Cytolyt is that name. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kemlo Rogerson Sent: Mon 3/21/2005 4:01 AM To: 'Dave Low'; Scholz, Stephen J.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thin-Prep[Scanned] I don't think you use the ThinPrep vial, I believe you use the one that is a preservative and lyses red blood cells, is it Cytolyse? It's in the manual. If you use the ThinPrep vial then you have problems with rbc's which become fixed. -----Original Message----- From: Dave Low [mailto:lowman034@yahoo.com] Sent: 18 March 2005 19:55 To: Scholz, Stephen J.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Thin-Prep[Scanned] Stephen, Yes, you can use the ThinPrep vial solution with your Non-Gyns. Here is the contact number: Cytyc Corporation Customer Service 85 Swanson Road Boxborough, MA 01719 1-800-442-9892 option 5 Good Luck! Dave Low Malcolm Grow Med Ctr --- "Scholz, Stephen J." wrote: > Hello all; > > I was instructed by a Pathologist that I work for to > investigate using the ThinPrep technology in a > non-gyne capacity. Is this done? I know that it is > used for gyne specimens but I have never heard of > using the technology for non-gyne. I'm a Histo-tech > and my experience with cytology is limited so any > information that I could get would be appreciated. > (where to get the instrument, cost, is it > applicable) > > Thank you, > > Stephen J. Scholz HT(ASCP) > > 815-395-5410 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From asmith <@t> mail.barry.edu Mon Mar 21 09:35:14 2005 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] AW: Automated Image analysis Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3CA3@exchsrv01.barrynet.barry.edu> When someone asks if a product exists, a discreet advertisement, such as Christof Krug's, is an appropriate response. In the case of a new product, it is the only possible response. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, March 15, 2005 4:02 PM To: 'Christof Krug' Cc: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] AW: Automated Image analysis This seems to be a blatant addvertisement! Christof this is not allowed on histonet Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Christof Krug [mailto:Christof.Krug@t-online.de] Sent: Wednesday, 16 March 2005 1:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AW: Automated Image analysis Hi Yan I have developed an automatic image analysis software (www.cvistec.com ) for complex biomedical images. It is best suited for high throughput, high content image analysis and tissue microarrays. Customized measurement solutions for your specific images can be add. If you are interested, please get in touch with me. Kind regards Chistof Krug Informatikb?ro Christof Krug Dominikstr.21 D-81929 M?nchen Phone +49 89 99341972 Mobil +49 170 5836734 Fax +49 89 99341974 Email info@cvistec.com WWW www.cvistec.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Janet.Bonner <@t> FLHOSP.ORG Mon Mar 21 09:44:55 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] RE: Industrial Sharpie good news Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41EB@fh2k093.fhmis.net> Gayle, We purchase the industrial sharpie from Office Depot. We only use them on the cassettes, not slides. We haven't had the problem of anyone grabbing the wrong pen, but we keep them in a separate place - pencil holder or drawer - depending on the area. We also use a cassette printer from TBS so we don't have to use the pen all of the time. For slides we use the Statmark because the industrial sharpie has been known to come off - for example, when we circle slides to indicate where we put the gelblock section, and it is put through the stainer and the Statmark pen has survived. Same story with labeling the slides - the industrial sharpie faded. Let me know if you need the Statmark information- Janet -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Friday, March 18, 2005 3:43 PM To: Bonner, Janet; Histonet@lists.utsouthwestern.edu Subject: Industrial Sharpie good news Dear Janet, Thank you for info about Industrial Sharpie ink staying on in solvents. Where are you purchasing these special Sharpies? Staples, or some other vendor? I was worried that someone inadvertently grabs a run of the mill, everyday Sharpie marker used for labeling bottles, tape, and label their tissue cassettes or slides! We have had this happen with disasterous results when a whole project's labeling is dissolved away because the unaware used the wrong Sharpie marker. I plan to give the industrial Sharpies a try though. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Janet.Bonner <@t> FLHOSP.ORG Mon Mar 21 10:05:35 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] .."Sharpie" marker pens Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41EC@fh2k093.fhmis.net> I wonder if it has something with the cassette manufacturer - maybe there is a coating - like oil? We use Sakura/Allegiance/Cardinal (whatever they're called now) with the industrial pen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonnie Whitaker Sent: Friday, March 18, 2005 2:19 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] .."Sharpie" marker pens It didn't work for me either! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Friday, March 18, 2005 1:01 PM To: Bonner, Janet; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Caveat about true "Sharpie" marker pens fortissue cassettes Re:Colored Cassettes for multi institution study The Sharpie "industrial" did not work in our processor. LuAnn At 11:37 AM 3/18/05, Bonner, Janet wrote: >The Sharpie "industrial" survives the processing chemicals but we use >the Statmark pen for the slides. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle >Callis >Sent: Friday, March 18, 2005 10:51 AM >To: John Robertson; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue >cassettes Re:Colored Cassettes for multi institution study > > >Caveat: True Sharpie marker, per se, ink is washed off by processing >solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost >Marker II pen that LOOK like "sharpie" markers but have special ink >that stays on plastic cassettes? > > At 02:34 PM 3/17/2005, you wrote: > > >Hal- You might consider a cassette bearing a large letter for each > >institution. A sequentially bar coded set would permit accurate > >sample logging and tracking. These markings could be put on the side > >to permit traditional sharpie markings on the face. Alternatively the > >face could be marked with large letters leaving room for the sharpie. > > > >See a picture at > >http://www.vialabel.com/bigletter.jpg > > > >Hope this helps Hal. > >John > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RBARNHART <@t> summithealth.org Mon Mar 21 10:14:38 2005 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] .."Sharpie" marker pens Message-ID: We have been able to use a sharpie industrial pen with Sakura, Ted Pella and SurgiPath cassettes. We don't use it on slides because it fades just enough to passably cause problems. >>> "Bonner, Janet" 3/21/2005 11:05:35 AM >>> I wonder if it has something with the cassette manufacturer - maybe there is a coating - like oil? We use Sakura/Allegiance/Cardinal (whatever they're called now) with the industrial pen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonnie Whitaker Sent: Friday, March 18, 2005 2:19 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] .."Sharpie" marker pens It didn't work for me either! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Friday, March 18, 2005 1:01 PM To: Bonner, Janet; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Caveat about true "Sharpie" marker pens fortissue cassettes Re:Colored Cassettes for multi institution study The Sharpie "industrial" did not work in our processor. LuAnn At 11:37 AM 3/18/05, Bonner, Janet wrote: >The Sharpie "industrial" survives the processing chemicals but we use >the Statmark pen for the slides. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle >Callis >Sent: Friday, March 18, 2005 10:51 AM >To: John Robertson; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue >cassettes Re:Colored Cassettes for multi institution study > > >Caveat: True Sharpie marker, per se, ink is washed off by processing >solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost >Marker II pen that LOOK like "sharpie" markers but have special ink >that stays on plastic cassettes? > > At 02:34 PM 3/17/2005, you wrote: > > >Hal- You might consider a cassette bearing a large letter for each > >institution. A sequentially bar coded set would permit accurate > >sample logging and tracking. These markings could be put on the side > >to permit traditional sharpie markings on the face. Alternatively the > >face could be marked with large letters leaving room for the sharpie. > > > >See a picture at > >http://www.vialabel.com/bigletter.jpg > > > >Hope this helps Hal. > >John > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Mon Mar 21 10:21:02 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Immunofluorescence in paraffin sections Message-ID: <20050321162103.66846.qmail@web15504.mail.cnb.yahoo.com> Hello, all, I am doing immunofluorescence in paraffin sections. I have some difficulties in it: 1: For pretreatment of tissue sections, I plan to incubate sections with trypsin solution, but I do not know :what kind of concentration should I choose ? (0.01% trypsin, 0.05% or o.1%)how long does it should last? (10min,20min,30 min) 2: Recently, I read a protocol about double immunofluorescence, it shows :Antibodies derived from different animal can be mixed and incubated as a cocktail (example: rabbit anti-A and mouse anti-B). The same is valid for secondary antibodies (example: goat anti-rabbit Texas Red conjugated and goat anti-mouse fluorescein conjugated). but If secondary antibodies cross-react, what should I do? My friend told me that I could use depletion method. but I am not sure. Can anybody do me a favor? Thank you! Guofeng --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From gcallis <@t> montana.edu Mon Mar 21 10:50:33 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re:Mouse histology books In-Reply-To: References: Message-ID: <6.0.0.22.1.20050321093939.01b66d38@gemini.msu.montana.edu> Tim, The only mouse histology book I know of that has color photos is Histological Atlas of the Laboratory Mouse, Gude, Cosgrove and Hirsch. 1982, ISBN 0-306-40686-1, and probably long out of print (and should be returned to the fold!) The photos are marginal at times (low power) or not the best quality, but it is better than nothing at all. There is another atlas, A colour atlas of Anatomy of Small Laboratory Animals, Volume 2: rat, Mouse Hamster Popesko, Rajtova and Horak from SaundersISBN 0 7020 2703 0, Reprint 2002. This is pricey and will NOT have photos for what you are looking for - everything is in diagram form and more for locating organs, structures, etc. There are websites with some photographs but you have to do some searching on line. Otherwise, there really is not much out there in terms of this species nor even a rat. Good luck on finding the first book. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Luis.Chiriboga <@t> med.nyu.edu Mon Mar 21 09:45:05 2005 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] NYSHS Call For Nominations 2005 (NYSHS Members Only) Message-ID: TO: All New York State Histotechnology Society Members RE: Award nominations for 2005 The NYSHS Awards committee is asking for nominations from its membership for this years awards cycle. In addition to recognition by the society, recipients will also be awarded a small financial grant to further their knowledge and education in the discipline of histotechnology. Applications are due by April 10th 2004 1.Thermo Electron Student Scholarship Award: Is awarded to a histology student or a histotech who wishes to attend a professional meeting. This award is sponsored by thermo Electron inc. and must be used to defray educational expenses.Please send us: 1.. A letter from you, showing evidence of your commitment to continuing education. 2.. Two letters of recommendation from supervisor, pathologists, or histotechnologist 3.. Name and address of your current employer or school, and your current address 2. Dominic Europa Award: Awarded to a long standing NYSHS member (for use towards an educational meeting) who serves as an inspiration to others in the field. Candidate can be a bench tech or a supervisor (preferably not in the limelight). Please submit a nomination and recommendation letter, detailing the nominees contributions . 3.Biogenex Excellence in Education Award: Awarded to any member in good standing, to be used to fund an educational endeavor. This endeavor could be tuition for a class, educational materials or payment for a meeting that is not funded by an employer. To apply for this award, please send a letter outlining how you would benefit educationally from this award, and how it will help you to better serve the profession. This award is sponsored by Biogenex. We encourage e-mail submission of applications and letters. Please submit applications to: Cindy Kosuda 4997 Henderson Street Whitesboro, NY 13492 Fax (315)624-4919 cindyk@centrexlabs.com From David.Edmondson <@t> christie-tr.nwest.nhs.uk Mon Mar 21 11:13:27 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Immunofluorescence in paraffin sections Message-ID: Hello, 1. I have no experience of using fluoresence BUT the pretreatments that = you use to reveal antigens should be analagous to other detection = systems. SINCE IT IS THE ANTIGENS THAT ARE IMPORTANT. So IF trypsin is the business then around 0.05% in 0.1% Calcium = Chloride, for say ten minutes woks for various antigens by = immunoperoxidase methods. If the fluoresence is a sensitive as one = imagines then times and concentrations may be shorter. The time that is best is what you find works best rather than what I = say. What about other pretreatments?=20 2. Can not help here Dave Histology Christie Hospital Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of = pex0220@yahoo.com.cn Sent: 21 March 2005 16:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence in paraffin sections Hello, all, =20 I am doing immunofluorescence in paraffin sections. I have some = difficulties in it: 1: For pretreatment of tissue sections, I plan to incubate sections with = trypsin solution, but I do not know :what kind of concentration should I = choose ? (0.01% trypsin, 0.05% or o.1%)how long does it should last? = (10min,20min,30 min) 2: Recently, I read a protocol about double immunofluorescence, it shows = :Antibodies derived from different animal can be mixed and incubated as = a cocktail (example: rabbit anti-A and mouse anti-B). The same is valid = for secondary antibodies (example: goat anti-rabbit Texas Red conjugated = and goat anti-mouse fluorescein conjugated). but If secondary antibodies = cross-react, what should I do? My friend told me that I could use = depletion method. but I am not sure. =20 Can anybody do me a favor? Thank you! =20 Guofeng =20 --------------------------------- Do You Yahoo!? =D7=A2=B2=E1=CA=C0=BD=E7=D2=BB=C1=F7=C6=B7=D6=CA=B5=C4=D1=C5=BB=A2=C3=E2=B7= =D1=B5=E7=D3=CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Mar 21 11:18:01 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Immunofluorescence in paraffin sections In-Reply-To: <20050321162103.66846.qmail@web15504.mail.cnb.yahoo.com> References: <20050321162103.66846.qmail@web15504.mail.cnb.yahoo.com> Message-ID: <6.0.0.22.1.20050321101213.01b0cb50@gemini.msu.montana.edu> Goat is not the only host for seconday antibodies. You could use Donkey antimouse - FITC. Try Jackson Immunoreasearch for other antibodies that will NOT cross react. Jackson has a website. At 09:21 AM 3/21/2005, you wrote: >Hello, all, > >I am doing immunofluorescence in paraffin sections. I have some >difficulties in it: >1: For pretreatment of tissue sections, I plan to incubate sections with >trypsin solution, but I do not know :what kind of concentration should I >choose ? (0.01% trypsin, 0.05% or o.1%)how long does it should last? >(10min,20min,30 min) >2: Recently, I read a protocol about double immunofluorescence, it shows >:Antibodies derived from different animal can be mixed and incubated as a >cocktail (example: rabbit anti-A and mouse anti-B). The same is valid for >secondary antibodies (example: goat anti-rabbit Texas Red conjugated and >goat anti-mouse fluorescein conjugated). but If secondary antibodies >cross-react, what should I do? My friend told me that I could use >depletion method. but I am not sure. > >Can anybody do me a favor? >Thank you! > >Guofeng > > > > >--------------------------------- >Do You Yahoo!? >?????????????????????????????? >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From sharon.osborn <@t> dnax.org Mon Mar 21 11:42:40 2005 From: sharon.osborn <@t> dnax.org (Osborn, Sharon) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re: Are Gloves a CAP regulation? Message-ID: <29B25753F6B1D51196110002A589D44402397F1D@PALMSG30.us.schp.com> As many of you, I have worked several lifetimes in histology including using bare hands to lift specimens out of formalin. However, I began wearing gloves in the early 1970's because I developed sensitivity to the formalin--fingers being numb, stinging sensations, etc. In these later years, I notice sensitivity to xylene--yes, it is slow going doing coverslipping with gloves when need to do hand 'slipping. I wear gloves for embedding because there is still some carryover of the xylene in the paraffins, even in the #3 paraffin. As we have become more educated about our hazardous, we know that xylene travels through our fatty tissue and lodges in the liver where it remains.. Nitrile gloves also resist xylene longer than latex. I don't wear gloves when microtoming paraffin sections unless it is for RNAse studies--then it is a requirement to keep my RNAse from contaminating the study material. I do wear gloves while using the cryostat. I had not noticed anyone mentioning sensitivity problems to the chemicals or the possible health hazards to us with absorbing the chemicals through our skin. We have plenty of people telling us about the smell of chemicals when they come by and that tends to be addressed more quickly. Sharon Osborn DNAX Palo Alto, CA ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From anthony.rosalia <@t> citigroup.com Mon Mar 21 12:14:41 2005 From: anthony.rosalia <@t> citigroup.com (Rosalia, Anthony [IIG]) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] H&E Stainers Message-ID: I really appreciate everyone's help so far, it has made our research on automating our lab much more efficient. I began looking at several H&E stainers and don't quit understand Ventana's Symphony product. It is very expensive ($150k) and I'm not sure why it is so much more than all the other comparable products out there. Am I missing something? Anthony Rosalia From je22r <@t> udcf.gla.ac.uk Mon Mar 21 12:20:52 2005 From: je22r <@t> udcf.gla.ac.uk (Julia Edgar) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re: Histonet Digest, Vol 16, Issue 34 References: Message-ID: <003001c52e42$b70d5270$cdead182@vet.gla.ac.uk> Prior to de-waxing, prepare a solution of 0.1% trypsin (e.g. Sigma-T-4799) in 0.1% Calcium chloride and warm to 37oC. It is important to make this solution fresh, on the day of use. Incubate sections for 20 minutes (or whatever works for your particular antibody/antigen). Wash thoroughly in PBS then proceed with blocking. Julia > I am doing immunofluorescence in paraffin sections. I have some difficulties in it: > 1: For pretreatment of tissue sections, I plan to incubate sections with trypsin solution, but I do not know :what kind of concentration should I choose ? (0.01% trypsin, 0.05% or o.1%)how long does it should last? (10min,20min,30 min) > 2: Recently, I read a protocol about double immunofluorescence, it shows :Antibodies derived from different animal can be mixed and incubated as a cocktail (example: rabbit anti-A and mouse anti-B). The same is valid for secondary antibodies (example: goat anti-rabbit Texas Red conjugated and goat anti-mouse fluorescein conjugated). but If secondary antibodies cross-react, what should I do? My friend told me that I could use depletion method. but I am not sure. > > Can anybody do me a favor? > Thank you! > > Guofeng From lhotaks <@t> mcmaster.ca Mon Mar 21 12:29:58 2005 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re: Immunofluorescence on paraffin sections Message-ID: Guofeng, For double immunofluorescence (IF) I first troubleshoot each antibody with peroxidase, and then use the same conditions (pretreatment, dilution) for IF. The trick is that both antibodies have to work well with the same pretreatment. This is not always possible. I would strongly recommend Alexa fluorochromes from Molecular probes instead of Texas Red, FITC etc. They are very stable and strong. Molecular probes carries all kind of colors so that you can match them to what filters you have available on your microscope. Also, they have them conjugated to immunoglobulins from various species. You can get donkey anti-mouse,-rabbit,-goat, etc. Then you can use normal donkey serum to block. Good luck, Sarka Lhotak McMaster University Hamilton, Ontario, Canada From ja.mitchell <@t> hosp.wisc.edu Mon Mar 21 13:13:47 2005 From: ja.mitchell <@t> hosp.wisc.edu (Mitchell Jean A.) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] 2005 Tri-State Meeting - Rochester, MN Message-ID: <583D3E9A1E843445BD54E461D1A2F6F30F83F559@uwhis-xchng2.hosp.wisc.edu> Come join the histology societies of Iowa, Minnesota & Wisconsin as they sponsor the: 2005 Tri-State Spring Histology Symposium Rockin', Rollin' & Histo-Twistin' in Rochester April 27-29, 2005 (Wednesday-Friday) Two Day Registration - $100 (includes all seminars, workshop fees, lunches, banquet) Banquet Theme - "Let's Go To the Histo-Hop" Hotel Rate - $79 + tax, Radisson Plaza Hotel, Rochester, Minnesota 8 Workshops offered: Preparing for the IHC Qualification Exam, Mystery Science Theater II; The Sequel, Troubleshooting Special Stains, An Enchanting Historical Survey of the Dyes We Use, Non-Isotopic In Situ PCR for Technologists by Technologists, What Every Histotech Should Know About Water, Conflict Management; The Courage to Confront, Application of Basic IHC, Let's Take the Bug Out of the Biels. 7 Seminars offered: How Does Quality Systems & Validation Slide into Histology, Hazardous Waste-Generation to Destruction, Preparing & Shipping Medical Specimens, The Importance of the Histotech in Forensic Medicine, Creuztfeld-Jacob Disease in the United Kingdom, Transmissible Spongiform Encepthalopathies: A Brief Prospective from the Animal World, Moh's Surgery; The Quintessential Margin Check For further information contact any of the following state chairpersons: Iowa - Judi Stasko; jstasko@nadc.ars.usda.gov Minnesota - Colleen Forster; cforster@tc.umn.edu Wisconsin - Jean Mitchell; ja.mitchell@hosp.wisc.edu From mclarke <@t> allsaintshealthcare.org Mon Mar 21 13:26:57 2005 From: mclarke <@t> allsaintshealthcare.org (Clarke, Mary) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] certification Message-ID: <3959B2255CA51443928A90517C973D6418EC58@WFEXBE04.wfsi.priv> I have student that has completed her two years of on the job training and is now ready to get certified. Unfortunately she has missed the cut off for taking the test, now that ASCP has changed the ways of taking the test. Does any one know of any ways that she can get certified. We would also be interested in any online courses. Thanks Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, March 21, 2005 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 34 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Thin-Prep[Scanned] (Kemlo Rogerson) 2. RE: Thin-Prep[Scanned] (Weems, Joyce) 3. RE: AW: Automated Image analysis (Smith, Allen) 4. RE: Industrial Sharpie good news (Bonner, Janet) 5. RE: .."Sharpie" marker pens (Bonner, Janet) 6. RE: .."Sharpie" marker pens (Rebecca Barnhart) 7. Immunofluorescence in paraffin sections (=?gb2312?q?=CC=EC=20=D0=C1?=) 8. Re:Mouse histology books (Gayle Callis) 9. NYSHS Call For Nominations 2005 (NYSHS Members Only) (Luis Chiriboga) 10. RE: Immunofluorescence in paraffin sections (Edmondson David (RBV) NHS Christie Tr) 11. Re: Immunofluorescence in paraffin sections (Gayle Callis) 12. Re: Are Gloves a CAP regulation? (Osborn, Sharon) ---------------------------------------------------------------------- Message: 1 Date: Mon, 21 Mar 2005 09:01:51 -0000 From: Kemlo Rogerson Subject: RE: [Histonet] Thin-Prep[Scanned] To: 'Dave Low' , "Scholz, Stephen J." , histonet@lists.utsouthwestern.edu Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0BB@bhrv-nt-11.bhrv.nwest.nhs.uk> Content-Type: text/plain I don't think you use the ThinPrep vial, I believe you use the one that is a preservative and lyses red blood cells, is it Cytolyse? It's in the manual. If you use the ThinPrep vial then you have problems with rbc's which become fixed. -----Original Message----- From: Dave Low [mailto:lowman034@yahoo.com] Sent: 18 March 2005 19:55 To: Scholz, Stephen J.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Thin-Prep[Scanned] Stephen, Yes, you can use the ThinPrep vial solution with your Non-Gyns. Here is the contact number: Cytyc Corporation Customer Service 85 Swanson Road Boxborough, MA 01719 1-800-442-9892 option 5 Good Luck! Dave Low Malcolm Grow Med Ctr --- "Scholz, Stephen J." wrote: > Hello all; > > I was instructed by a Pathologist that I work for to > investigate using the ThinPrep technology in a > non-gyne capacity. Is this done? I know that it is > used for gyne specimens but I have never heard of > using the technology for non-gyne. I'm a Histo-tech > and my experience with cytology is limited so any > information that I could get would be appreciated. > (where to get the instrument, cost, is it > applicable) > > Thank you, > > Stephen J. Scholz HT(ASCP) > > 815-395-5410 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 21 Mar 2005 08:48:12 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Thin-Prep[Scanned] To: "Kemlo Rogerson" , "Dave Low" , "Scholz, Stephen J." , Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D21C@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" There are non-gyn supplies - Cytolyt is that name. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kemlo Rogerson Sent: Mon 3/21/2005 4:01 AM To: 'Dave Low'; Scholz, Stephen J.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thin-Prep[Scanned] I don't think you use the ThinPrep vial, I believe you use the one that is a preservative and lyses red blood cells, is it Cytolyse? It's in the manual. If you use the ThinPrep vial then you have problems with rbc's which become fixed. -----Original Message----- From: Dave Low [mailto:lowman034@yahoo.com] Sent: 18 March 2005 19:55 To: Scholz, Stephen J.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Thin-Prep[Scanned] Stephen, Yes, you can use the ThinPrep vial solution with your Non-Gyns. Here is the contact number: Cytyc Corporation Customer Service 85 Swanson Road Boxborough, MA 01719 1-800-442-9892 option 5 Good Luck! Dave Low Malcolm Grow Med Ctr --- "Scholz, Stephen J." wrote: > Hello all; > > I was instructed by a Pathologist that I work for to > investigate using the ThinPrep technology in a > non-gyne capacity. Is this done? I know that it is > used for gyne specimens but I have never heard of > using the technology for non-gyne. I'm a Histo-tech > and my experience with cytology is limited so any > information that I could get would be appreciated. > (where to get the instrument, cost, is it > applicable) > > Thank you, > > Stephen J. Scholz HT(ASCP) > > 815-395-5410 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 3 Date: Mon, 21 Mar 2005 10:35:14 -0500 From: "Smith, Allen" Subject: RE: [Histonet] AW: Automated Image analysis To: "Tony Henwood" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3CA3@exchsrv01.barrynet.barry.edu> Content-Type: text/plain; charset="iso-8859-1" When someone asks if a product exists, a discreet advertisement, such as Christof Krug's, is an appropriate response. In the case of a new product, it is the only possible response. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, March 15, 2005 4:02 PM To: 'Christof Krug' Cc: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] AW: Automated Image analysis This seems to be a blatant addvertisement! Christof this is not allowed on histonet Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Christof Krug [mailto:Christof.Krug@t-online.de] Sent: Wednesday, 16 March 2005 1:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AW: Automated Image analysis Hi Yan I have developed an automatic image analysis software (www.cvistec.com ) for complex biomedical images. It is best suited for high throughput, high content image analysis and tissue microarrays. Customized measurement solutions for your specific images can be add. If you are interested, please get in touch with me. Kind regards Chistof Krug Informatikb?ro Christof Krug Dominikstr.21 D-81929 M?nchen Phone +49 89 99341972 Mobil +49 170 5836734 Fax +49 89 99341974 Email info@cvistec.com WWW www.cvistec.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) ------------------------------ Message: 4 Date: Mon, 21 Mar 2005 10:44:55 -0500 From: "Bonner, Janet" Subject: [Histonet] RE: Industrial Sharpie good news To: "'Gayle Callis'" , "Bonner, Janet" , Histonet@lists.utsouthwestern.edu Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41EB@fh2k093.fhmis.net> Content-Type: text/plain; charset="iso-8859-1" Gayle, We purchase the industrial sharpie from Office Depot. We only use them on the cassettes, not slides. We haven't had the problem of anyone grabbing the wrong pen, but we keep them in a separate place - pencil holder or drawer - depending on the area. We also use a cassette printer from TBS so we don't have to use the pen all of the time. For slides we use the Statmark because the industrial sharpie has been known to come off - for example, when we circle slides to indicate where we put the gelblock section, and it is put through the stainer and the Statmark pen has survived. Same story with labeling the slides - the industrial sharpie faded. Let me know if you need the Statmark information- Janet -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Friday, March 18, 2005 3:43 PM To: Bonner, Janet; Histonet@lists.utsouthwestern.edu Subject: Industrial Sharpie good news Dear Janet, Thank you for info about Industrial Sharpie ink staying on in solvents. Where are you purchasing these special Sharpies? Staples, or some other vendor? I was worried that someone inadvertently grabs a run of the mill, everyday Sharpie marker used for labeling bottles, tape, and label their tissue cassettes or slides! We have had this happen with disasterous results when a whole project's labeling is dissolved away because the unaware used the wrong Sharpie marker. I plan to give the industrial Sharpies a try though. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 5 Date: Mon, 21 Mar 2005 11:05:35 -0500 From: "Bonner, Janet" Subject: RE: [Histonet] .."Sharpie" marker pens To: "'Bonnie Whitaker'" , Histonet@lists.utsouthwestern.edu Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41EC@fh2k093.fhmis.net> Content-Type: text/plain; charset="iso-8859-1" I wonder if it has something with the cassette manufacturer - maybe there is a coating - like oil? We use Sakura/Allegiance/Cardinal (whatever they're called now) with the industrial pen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonnie Whitaker Sent: Friday, March 18, 2005 2:19 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] .."Sharpie" marker pens It didn't work for me either! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Friday, March 18, 2005 1:01 PM To: Bonner, Janet; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Caveat about true "Sharpie" marker pens fortissue cassettes Re:Colored Cassettes for multi institution study The Sharpie "industrial" did not work in our processor. LuAnn At 11:37 AM 3/18/05, Bonner, Janet wrote: >The Sharpie "industrial" survives the processing chemicals but we use >the Statmark pen for the slides. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle >Callis >Sent: Friday, March 18, 2005 10:51 AM >To: John Robertson; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue >cassettes Re:Colored Cassettes for multi institution study > > >Caveat: True Sharpie marker, per se, ink is washed off by processing >solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost >Marker II pen that LOOK like "sharpie" markers but have special ink >that stays on plastic cassettes? > > At 02:34 PM 3/17/2005, you wrote: > > >Hal- You might consider a cassette bearing a large letter for each > >institution. A sequentially bar coded set would permit accurate > >sample logging and tracking. These markings could be put on the side > >to permit traditional sharpie markings on the face. Alternatively the > >face could be marked with large letters leaving room for the sharpie. > > > >See a picture at > >http://www.vialabel.com/bigletter.jpg > > > >Hope this helps Hal. > >John > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 21 Mar 2005 11:14:38 -0500 From: "Rebecca Barnhart" Subject: RE: [Histonet] .."Sharpie" marker pens To: Message-ID: Content-Type: text/plain; charset=US-ASCII We have been able to use a sharpie industrial pen with Sakura, Ted Pella and SurgiPath cassettes. We don't use it on slides because it fades just enough to passably cause problems. >>> "Bonner, Janet" 3/21/2005 11:05:35 AM >>> I wonder if it has something with the cassette manufacturer - maybe there is a coating - like oil? We use Sakura/Allegiance/Cardinal (whatever they're called now) with the industrial pen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonnie Whitaker Sent: Friday, March 18, 2005 2:19 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] .."Sharpie" marker pens It didn't work for me either! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Friday, March 18, 2005 1:01 PM To: Bonner, Janet; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Caveat about true "Sharpie" marker pens fortissue cassettes Re:Colored Cassettes for multi institution study The Sharpie "industrial" did not work in our processor. LuAnn At 11:37 AM 3/18/05, Bonner, Janet wrote: >The Sharpie "industrial" survives the processing chemicals but we use >the Statmark pen for the slides. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle >Callis >Sent: Friday, March 18, 2005 10:51 AM >To: John Robertson; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Caveat about true "Sharpie" marker pens for tissue >cassettes Re:Colored Cassettes for multi institution study > > >Caveat: True Sharpie marker, per se, ink is washed off by processing >solvents. Are you refering to the STATMARK PEN (StatLab) or Superfrost >Marker II pen that LOOK like "sharpie" markers but have special ink >that stays on plastic cassettes? > > At 02:34 PM 3/17/2005, you wrote: > > >Hal- You might consider a cassette bearing a large letter for each > >institution. A sequentially bar coded set would permit accurate > >sample logging and tracking. These markings could be put on the side > >to permit traditional sharpie markings on the face. Alternatively the > >face could be marked with large letters leaving room for the sharpie. > > > >See a picture at > >http://www.vialabel.com/bigletter.jpg > > > >Hope this helps Hal. > >John > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 22 Mar 2005 00:21:02 +0800 (CST) From: =?gb2312?q?=CC=EC=20=D0=C1?= Subject: [Histonet] Immunofluorescence in paraffin sections To: histonet@lists.utsouthwestern.edu Message-ID: <20050321162103.66846.qmail@web15504.mail.cnb.yahoo.com> Content-Type: text/plain; charset=gb2312 Hello, all, I am doing immunofluorescence in paraffin sections. I have some difficulties in it: 1: For pretreatment of tissue sections, I plan to incubate sections with trypsin solution, but I do not know :what kind of concentration should I choose ? (0.01% trypsin, 0.05% or o.1%)how long does it should last? (10min,20min,30 min) 2: Recently, I read a protocol about double immunofluorescence, it shows :Antibodies derived from different animal can be mixed and incubated as a cocktail (example: rabbit anti-A and mouse anti-B). The same is valid for secondary antibodies (example: goat anti-rabbit Texas Red conjugated and goat anti-mouse fluorescein conjugated). but If secondary antibodies cross-react, what should I do? My friend told me that I could use depletion method. but I am not sure. Can anybody do me a favor? Thank you! Guofeng --------------------------------- Do You Yahoo!? ?????????????????????????????? ------------------------------ Message: 8 Date: Mon, 21 Mar 2005 09:50:33 -0700 From: Gayle Callis Subject: [Histonet] Re:Mouse histology books To: Timothy Macatee , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050321093939.01b66d38@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Tim, The only mouse histology book I know of that has color photos is Histological Atlas of the Laboratory Mouse, Gude, Cosgrove and Hirsch. 1982, ISBN 0-306-40686-1, and probably long out of print (and should be returned to the fold!) The photos are marginal at times (low power) or not the best quality, but it is better than nothing at all. There is another atlas, A colour atlas of Anatomy of Small Laboratory Animals, Volume 2: rat, Mouse Hamster Popesko, Rajtova and Horak from SaundersISBN 0 7020 2703 0, Reprint 2002. This is pricey and will NOT have photos for what you are looking for - everything is in diagram form and more for locating organs, structures, etc. There are websites with some photographs but you have to do some searching on line. Otherwise, there really is not much out there in terms of this species nor even a rat. Good luck on finding the first book. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 9 Date: Mon, 21 Mar 2005 10:45:05 -0500 From: Luis Chiriboga Subject: [Histonet] NYSHS Call For Nominations 2005 (NYSHS Members Only) To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" TO: All New York State Histotechnology Society Members RE: Award nominations for 2005 The NYSHS Awards committee is asking for nominations from its membership for this years awards cycle. In addition to recognition by the society, recipients will also be awarded a small financial grant to further their knowledge and education in the discipline of histotechnology. Applications are due by April 10th 2004 1.Thermo Electron Student Scholarship Award: Is awarded to a histology student or a histotech who wishes to attend a professional meeting. This award is sponsored by thermo Electron inc. and must be used to defray educational expenses.Please send us: 1.. A letter from you, showing evidence of your commitment to continuing education. 2.. Two letters of recommendation from supervisor, pathologists, or histotechnologist 3.. Name and address of your current employer or school, and your current address 2. Dominic Europa Award: Awarded to a long standing NYSHS member (for use towards an educational meeting) who serves as an inspiration to others in the field. Candidate can be a bench tech or a supervisor (preferably not in the limelight). Please submit a nomination and recommendation letter, detailing the nominees contributions . 3.Biogenex Excellence in Education Award: Awarded to any member in good standing, to be used to fund an educational endeavor. This endeavor could be tuition for a class, educational materials or payment for a meeting that is not funded by an employer. To apply for this award, please send a letter outlining how you would benefit educationally from this award, and how it will help you to better serve the profession. This award is sponsored by Biogenex. We encourage e-mail submission of applications and letters. Please submit applications to: Cindy Kosuda 4997 Henderson Street Whitesboro, NY 13492 Fax (315)624-4919 cindyk@centrexlabs.com ------------------------------ Message: 10 Date: Mon, 21 Mar 2005 17:13:27 -0000 From: "Edmondson David \(RBV\) NHS Christie Tr" Subject: RE: [Histonet] Immunofluorescence in paraffin sections To: "? ?" Cc: "Histonet \(E-mail 2\)" Message-ID: Content-Type: text/plain; charset="gb2312" Hello, 1. I have no experience of using fluoresence BUT the pretreatments that you use to reveal antigens should be analagous to other detection systems. SINCE IT IS THE ANTIGENS THAT ARE IMPORTANT. So IF trypsin is the business then around 0.05% in 0.1% Calcium Chloride, for say ten minutes woks for various antigens by immunoperoxidase methods. If the fluoresence is a sensitive as one imagines then times and concentrations may be shorter. The time that is best is what you find works best rather than what I say. What about other pretreatments? 2. Can not help here Dave Histology Christie Hospital Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of pex0220@yahoo.com.cn Sent: 21 March 2005 16:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence in paraffin sections Hello, all, I am doing immunofluorescence in paraffin sections. I have some difficulties in it: 1: For pretreatment of tissue sections, I plan to incubate sections with trypsin solution, but I do not know :what kind of concentration should I choose ? (0.01% trypsin, 0.05% or o.1%)how long does it should last? (10min,20min,30 min) 2: Recently, I read a protocol about double immunofluorescence, it shows :Antibodies derived from different animal can be mixed and incubated as a cocktail (example: rabbit anti-A and mouse anti-B). The same is valid for secondary antibodies (example: goat anti-rabbit Texas Red conjugated and goat anti-mouse fluorescein conjugated). but If secondary antibodies cross-react, what should I do? My friend told me that I could use depletion method. but I am not sure. Can anybody do me a favor? Thank you! Guofeng --------------------------------- Do You Yahoo!? ?????????????????????????????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 21 Mar 2005 10:18:01 -0700 From: Gayle Callis Subject: Re: [Histonet] Immunofluorescence in paraffin sections To: =?gb2312?q?=CC=EC=20=D0=C1?= , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050321101213.01b0cb50@gemini.msu.montana.edu> Content-Type: text/plain; charset="iso-8859-1"; format=flowed Goat is not the only host for seconday antibodies. You could use Donkey antimouse - FITC. Try Jackson Immunoreasearch for other antibodies that will NOT cross react. Jackson has a website. At 09:21 AM 3/21/2005, you wrote: >Hello, all, > >I am doing immunofluorescence in paraffin sections. I have some >difficulties in it: >1: For pretreatment of tissue sections, I plan to incubate sections with >trypsin solution, but I do not know :what kind of concentration should I >choose ? (0.01% trypsin, 0.05% or o.1%)how long does it should last? >(10min,20min,30 min) >2: Recently, I read a protocol about double immunofluorescence, it shows >:Antibodies derived from different animal can be mixed and incubated as a >cocktail (example: rabbit anti-A and mouse anti-B). The same is valid for >secondary antibodies (example: goat anti-rabbit Texas Red conjugated and >goat anti-mouse fluorescein conjugated). but If secondary antibodies >cross-react, what should I do? My friend told me that I could use >depletion method. but I am not sure. > >Can anybody do me a favor? >Thank you! > >Guofeng > > > > >--------------------------------- >Do You Yahoo!? >?????????????????????????????? >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 12 Date: Mon, 21 Mar 2005 12:42:40 -0500 From: "Osborn, Sharon" Subject: [Histonet] Re: Are Gloves a CAP regulation? To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <29B25753F6B1D51196110002A589D44402397F1D@PALMSG30.us.schp.com> Content-Type: text/plain As many of you, I have worked several lifetimes in histology including using bare hands to lift specimens out of formalin. However, I began wearing gloves in the early 1970's because I developed sensitivity to the formalin--fingers being numb, stinging sensations, etc. In these later years, I notice sensitivity to xylene--yes, it is slow going doing coverslipping with gloves when need to do hand 'slipping. I wear gloves for embedding because there is still some carryover of the xylene in the paraffins, even in the #3 paraffin. As we have become more educated about our hazardous, we know that xylene travels through our fatty tissue and lodges in the liver where it remains.. Nitrile gloves also resist xylene longer than latex. I don't wear gloves when microtoming paraffin sections unless it is for RNAse studies--then it is a requirement to keep my RNAse from contaminating the study material. I do wear gloves while using the cryostat. I had not noticed anyone mentioning sensitivity problems to the chemicals or the possible health hazards to us with absorbing the chemicals through our skin. We have plenty of people telling us about the smell of chemicals when they come by and that tends to be addressed more quickly. Sharon Osborn DNAX Palo Alto, CA ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 34 **************************************** From eshields <@t> bhset.org Mon Mar 21 13:47:05 2005 From: eshields <@t> bhset.org (E Sharon Shields) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re: sudan black Message-ID: does any one have a staining procedure for bone marrow aspirates for sudan black to distinguish acute myelocytic leukemia from other leukemia? Thank you for your help. Pathology Special Procedures Lab Baptist Hospital of East Tennessee Knoxville,TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail message, including any attachments, may contain confidential and privileged information that is protected by law. It is intended for the sole use of the recipient named above. If you are not the intended recipient or the agent responsible for delivering it to the intended recipient, you are hereby notified that any unauthorized review, use, dissemination or copying is strictly prohibited. If you have received this electronic mail transmission in error please notify us immediately at bellis@bhset.org and delete any copies from your system. <<<>>> From JMacDonald <@t> mtsac.edu Mon Mar 21 13:59:03 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] certification In-Reply-To: <3959B2255CA51443928A90517C973D6418EC58@WFEXBE04.wfsi.priv> Message-ID: She can enroll in a NAACLS accredited program. There are some programs that are certificate, others are degree programs. The certificate programs usually have minimum qualifications. See http://www.nsh.org/education/schools.html Her other option is to obtain an Associate Degree. See below for ASCP requirements To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Successful completion of a NAACLS accredited Histotechnician program. Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology within the last ten years under the supervision of a pathologist ( certified by the American Board of Pathology in Anatomic Pathology, or eligible), or an appropriately certified medical scientist. From GDawson <@t> dynacaremilwaukee.com Mon Mar 21 14:12:24 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Are Gloves a CAP regulation? Message-ID: Noooooo, Don't even speak of gloves and CAP regulation in the same breath. I can already see the CAP wheels turning. Soon we'll have to document that each glove does, in fact, have 5 fingers. I'm sure we'll need to document how many tear while putting them on or if they have excessive amounts of powder in them. A detailed pie chart on how rumpled up they are when they are taken off will be an absolute requirement to keep the laboratory walls from coming down. A new kit to measure exact purity of latex (or non-latex) content of each discarded glove will be available shortly. Long story short, every time I think it isn't possible to find more things to document for CAP, more pop up on their own so we don't need to throw out suggestions. Glen Dawson Milwaukee, WI From frosscis <@t> usc.edu Mon Mar 21 15:00:18 2005 From: frosscis <@t> usc.edu (Fred Ross-Cisneros) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Immunoperoxidase with DAB (Single Labeling) and Histofluorescence (Double labeling) Protocol Message-ID: <000001c52e58$fcd03370$7f607d80@sadunlab> Dear All, Could someone be so kind to share or advise me on a protocol for both immunoperoxidase using DAB (single labeling) and immunofluorescence (using double labels) on formalin-fixed paraffin-embedded (FFPE) human brain and optic nerve tissues? I need to identify myelin basic protein (MBP), glial fibrillary acidic protein (GFAP), neurofilaments (NF), and ED-1 for macrophages. I will do the immunofluorescence on a confocal microscope. Thank you. Fred Fred N. Ross-Cisneros Neuro-Ophthalmology Lab USC Keck School of Medicine and Doheny Eye Institute 1355 San Pablo Street, DVRC 311 Los Angeles, CA 90033-1026 Tel.: (323) 442-6667 Fax: (323) 442-6688 From kaleid11 <@t> yahoo.com Mon Mar 21 15:03:29 2005 From: kaleid11 <@t> yahoo.com (Adam Perry) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re: Problems with Gold-immunolabeling and silver enhancement in brain tissue In-Reply-To: <6.0.0.22.1.20050321090528.01b576d8@gemini.msu.montana.edu> Message-ID: <20050321210329.39197.qmail@web31614.mail.mud.yahoo.com> Thanks Gayle for the protocol and suggestions...looking it (and other protocols) over I had two more questions: 1) why are gold conjugated Abs light sensitive? Your protocol has the incubation in secondary in the dark...I can understand why some silver developers are light sensitive...but what's the reasoning behind keeping the secondary incubation in the dark? 2) are gold conjugated Abs more sensitive to detergents? Your protocol has both Ab incubations in PBS (no detergent)...and another protocol for immunogold had the primary in PBS with detergent..and then specified (!!!) no detergent in incubation with the gold-labeled secondary...For all my other ICC (usually avidin-biotin-peroxidase detection) I have detergent in every incubation step... I'm planning on doing another run to test the effects of light exposure versus foil wrapping and detergent versus no detergent, so maybe that will shed some more light on the source of my immunogold problems...I'm also going to take up your suggestion of contacting Chris van der loos, I just wanted to broaden my base for answers by including my questions to the Histonet subscribers... and if anyone has encountered any problems with immunogold labeling for light microscopy please let me know what they were and how you solved them... Thanks again for all the help... Adam Department of Physiology and Biophysics University of Illinois Chicago, IL --- Gayle Callis wrote: > Enhanced cold particles were aways very black and I > used a nuclear fast red > counterstain. I do not recall ever getting the > colors you describe. I > purchased some of my BBI gold conjugates (1 nm size > particles) from Vector > but they do not seem to have these anymore. I do > not do the kind of > immunostaining you do ( transcription factor in > brain) so I couldn't > comment on any of that in terms of immunogold. > > If you have an epiillumination microscope, take a > look at your sections. I > strongly suggest you visit with Chris van der Loos > on this, I think he can > help you with this more than I can - it really has > been a long time ago > since I did it. I have attached my IGGS protocol, > but if you need silver > enhancing reagents, I would have to retype these. I > do not have these on > file as of now. Mine was a silver acetate/ citrate > buffer, and very dicey > to work with, if the enhancement needed longer time, > I would have to go to > another fresh enhancing solution, as it broke down > rapidly, and often three > changes. These reagents were very fresh before > use. I used o close and > lock my door plus never answer a phone when I did > this step. > > Attached is an old IGGS protocol I used, just for > comparison sake. I > remember having stringent requirements for the > buffers I used. > > A tough call, but talk to Chris - he had great > success with his IGGS, and > he combined it with a double stain, for very nice > colocalization purposes, > spectacular. > > > > > At 02:44 PM 3/18/2005, you wrote: > >I actually started with in house prepared > >reagents...tried every protocol I could get my > hands > >on and none of them really worked well and all were > >time consuming to prepare...so I broke down and > bought > >a kit (from Aurion) and it was much easier to use, > but > >didn't seem to work. I haven't tried the > >epi-illumination scope yet though. > > > >What did your tissue sections look like after IGGS? > >In the original lab I trained in, the sections > would > >go through a color change from yellow, to brown, to > >greenish brown and then we'd stop the silver > >enhancement. The antigen I'm staining for is CREB > >(transcription factor found all throughout the > brain), > >so that may be why the entire section went through > the > >color change...but the staining I did with the > Aurion > >kit...the tissue looks amazingly clean, no > background > >staining...or anything...is that typical with IGGS? > > > >Thanks again, > >Adam > >Department of Physiology and Biophysics > >University of Illinois > >Chicago, IL > >--- Gayle Callis wrote: > > > How are you doing the silver enhancement, from > > > inhouse prepared > > > reagents? We found making up enhancement > reagents > > > inhouse a lot of work, > > > then it was hard to control the enhancement, a > > > painstaking procedure. > > > > > > Dr. Chris van der Loos uses a silver enhancement > kit > > > from > > > http://www.aurion.nl . When I visited his lab, > this > > > was a snap to use the > > > Aurion kit and we had excellent results. For > > > visualizing IGGS staining, > > > epi illumination is superior than trying to find > the > > > black enhanced > > > particles via standard transmitted light > microscopy. > > > You may have > > > staining, but can't see it very well - however > with > > > epi-illumination, the > > > enhanced gold particles show up like little > light > > > bulbs!! > > > > > > You can also ask him about his immunogold > methods at > > > > > > . > > > > > > At 12:16 PM 3/18/2005, you wrote: > > > >I'm trying to perform silver enhancement of my > gold > > > >labeled secondary antibody (1.4nm gold) in an > > > >immunocytochemistry (ICC) protocol in rodent > brain > > > >tissue. I know that the primary antibody and > > > initial > > > >steps are working because when I use an > > > >avidin-biotinylated peroxidase kit to visualize > a > > > >biotinylated secondary antibody (as opposed to > > > using > > > >the gold conjugated secondary antibody)I get > great > > > >labeling of my antigen. I've done a dot blot > with > > > the > > > >gold labeled antibody and I seem to get silver > > > >enhancement of particles only in the dot blot > with > > > the > > > >gold-labeled antibody (as opposed to unlabeled > > > >antibodies)- so I'm pretty sure there are gold > > > >particles attached to the IgG. > > > > > > > >Are there any obvious differences that need to > be > > > >considered when working with a gold-conjugated > > > >antibody as opposed to antibodies that are > > > >biotinylated or conjugated to enzymes?? > > > > > > > >Any insights would be greatly appreciated.. > > > >Thanks, > > > >Adam Perry > > > >Department of Physiology and Biophysics > > > >University of Illinois > > > >Chicago, IL > > > > > > > > >__________________________________________________ > > > >Do You Yahoo!? > > > >Tired of spam? Yahoo! Mail has the best spam > > > protection around > > > >http://mail.yahoo.com > > > > > > > >_______________________________________________ > > > >Histonet mailing list > > > >Histonet@lists.utsouthwestern.edu > > > > > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > Gayle Callis > > > MT,HT,HTL(ASCP) > > > Research Histopathology Supervisor > > > Veterinary Molecular Biology > > > Montana State University - Bozeman > > > PO Box 173610 > > > Bozeman MT 59717-3610 > > > 406 994-6367 (lab with voice mail) > > > 406 994-4303 (FAX) > > > > > > > > > > > > > > > > >__________________________________ > >Do you Yahoo!? > >Make Yahoo! your home page > >http://www.yahoo.com/r/hs > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > __________________________________ Do you Yahoo!? Make Yahoo! your home page http://www.yahoo.com/r/hs From kelly.mcqueeney <@t> bms.com Mon Mar 21 15:24:59 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Dako CSAII vs Elite ABC method, which is more sensitive? Message-ID: <423F3BAB.6060906@bms.com> I have been using Elite ABC kit for c-fos IHC in brain. We are getting pretty good staining and want to improve sensitivity. I compared the Elite ABC kit, Dako's CSA tyramide amplification kit, and Dako's Envison + kit. The sections are slide-mounted, 20 micron (I increased Dako's incubation times to 45 minutes). Dako claims that the tyramide and Envision kit are much more sensitive than Elite ABC. I have used the tyramide system in the past and was really impressed with the increased signal. Unfortuantely, Dako's CSA and Envision kit exhibit very weak staining and the Elite ABC kit exhibits dark and specific staining in the brain. Has anyone else noticed a change in either product? Should I order HRP-conjugated c-fos and amplify the signal? Any suggestions (besides floating sections)? Method: Quench w/Dako's H2O2, block 1 hour 3% BSA in PBT, c-fos O/N, wash buffer is PBS + 0.01% Tween-20 (PBT), nickel-DAB from Vector. Thanks, Kelly Bristol-Myers Squibb From lowman034 <@t> yahoo.com Mon Mar 21 17:07:36 2005 From: lowman034 <@t> yahoo.com (Dave Low) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Thin-Prep[Scanned] In-Reply-To: 6667 Message-ID: <20050321230737.35686.qmail@web40902.mail.yahoo.com> Cytolyt is correct! Thank you Joyce and Kemlo Rogerson is correct in his e-mail below. --- "Weems, Joyce" wrote: > There are non-gyn supplies - Cytolyt is that name. j > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on > behalf of Kemlo Rogerson > Sent: Mon 3/21/2005 4:01 AM > To: 'Dave Low'; Scholz, Stephen J.; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Thin-Prep[Scanned] > > I don't think you use the ThinPrep vial, I believe > you use the one that is a > preservative and lyses red blood cells, is it > Cytolyse? It's in the manual. > If you use the ThinPrep vial then you have problems > with rbc's which become > fixed. > > -----Original Message----- > From: Dave Low [mailto:lowman034@yahoo.com] > Sent: 18 March 2005 19:55 > To: Scholz, Stephen J.; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Thin-Prep[Scanned] > > Stephen, > > Yes, you can use the ThinPrep vial solution with > your > Non-Gyns. Here is the contact number: > > Cytyc Corporation > Customer Service > 85 Swanson Road > Boxborough, MA 01719 > 1-800-442-9892 option 5 > > Good Luck! > > Dave Low > Malcolm Grow Med Ctr > > > --- "Scholz, Stephen J." > wrote: > > Hello all; > > > > I was instructed by a Pathologist that I work for > to > > investigate using the ThinPrep technology in a > > non-gyne capacity. Is this done? I know that it > is > > used for gyne specimens but I have never heard of > > using the technology for non-gyne. I'm a > Histo-tech > > and my experience with cytology is limited so any > > information that I could get would be appreciated. > > > (where to get the instrument, cost, is it > > applicable) > > > > Thank you, > > > > Stephen J. Scholz HT(ASCP) > > > > 815-395-5410 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > __________________________________ > Do you Yahoo!? > Yahoo! Small Business - Try our new resources site! > http://smallbusiness.yahoo.com/resources/ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice ** The information contained > in this message may be privileged and is > confidential information intended for the use of the > addressee listed above. If you are neither the > intended recipient nor the employee or agent > responsible for delivering this message to the > intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of > any action in reliance on the contents of this > information is strictly prohibited. If you have > received this communication in error, please notify > us immediately by replying to the message and > deleting it from your computer. > Thank you. Saint Josephs Health System, Inc. > > __________________________________ Do you Yahoo!? Make Yahoo! your home page http://www.yahoo.com/r/hs From gcallis <@t> montana.edu Mon Mar 21 17:45:59 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Using Tween 20 with my IGSS method In-Reply-To: <20050321210329.39197.qmail@web31614.mail.mud.yahoo.com> References: <6.0.0.22.1.20050321090528.01b576d8@gemini.msu.montana.edu> <20050321210329.39197.qmail@web31614.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20050321151009.01b61480@gemini.msu.montana.edu> Adam, I went back and reviewed my IGSS methods, and why I had used Tween in some steps and then not with secondary and rest of protocol. At 02:03 PM 3/21/2005, you wrote: >Thanks Gayle for the protocol and >suggestions...looking it (and other protocols) over I >had two more questions: > >1) why are gold conjugated Abs light sensitive? Your >protocol has the incubation in secondary in the >dark...I can understand why some silver developers are >light sensitive...but what's the reasoning behind >keeping the secondary incubation in the dark? **** I think that is a typo error when I typed up that protocol flow sheet. Protection from light was when silver enhancer was made up and subsequently used. Somehow, I don't recall ever protecting gold conjugated secondary antibodies from the light but if I did, it would not have hurt what I did. >2) are gold conjugated Abs more sensitive to >detergents? Your protocol has both Ab incubations in >PBS (no detergent)...and another protocol for >immunogold had the primary in PBS with detergent..and >then specified (!!!) no detergent in incubation with >the gold-labeled secondary...For all my other ICC >(usually avidin-biotin-peroxidase detection) I have >detergent in every incubation step... I was working with the Microprobe system (mentioned in Step 1 of protocol) to do primary incubations, and all steps before and with primary antibody application had to contain Tween 20 in order to make the capillary gap work. After rinsing (via wicking, etc) primary away with buffer containing Tween 20, I got rid of the detergent to finish out the protocol without it. The secondary diluent did not contain Tween nor did the rest of the protocol. It is important to reduce ionic interactions protein protein with IGSS to prevent background but my use of detergents was not for that purpose. If I had to do all this over again, I think I would go purist, and do IGSS without detergent involved, just stringent buffer/diluents/gelatin/BSA additives seen with IGSS staining methods. My hard copy file folder floweth over with information! I have this article by van der Loos CM and Becker, J Histochem Cytochem 42(3):289-295, 1994 (a superb article about epi illumination with a double stain using IGSS and IHC alk phos methods). Stirling did a large study on detergents with IGSS, published in J Histotechnology. If you have a chance and can find it, there is a whole special issue on doing immunogold staining in J Histotechnology. Not sure if Tween really makes a difference in success of IGSS, maybe it does. I would like to try Aurion enhancing kit for 5 to 15 minutes versus the long, painfully (closed doors, never answer phone!) tedious silver enhancement method I did using inhouse reagents. I keep encouraging my immunologist to try IGSS, but he doesn't have epi illumination which is truly the ideal way to view these stained sections over standard light microscopy. >I'm planning on doing another run to test the effects >of light exposure versus foil wrapping and detergent >versus no detergent, so maybe that will shed some more >light on the source of my immunogold problems...I'm >also going to take up your suggestion of contacting >Chris van der loos, I just wanted to broaden my base >for answers by including my questions to the Histonet >subscribers... > >and if anyone has encountered any problems with >immunogold labeling for light microscopy please let me >know what they were and how you solved them... > >Thanks again for all the help... >Adam >Department of Physiology and Biophysics >University of Illinois >Chicago, IL > >- Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Kharazia <@t> egcrc.net Mon Mar 21 20:13:00 2005 From: Kharazia <@t> egcrc.net (Viktor Kharazia) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] static on slides & Cryostat Message-ID: we observed that cryosections (~10um) would stop jumping onto (or away from) the approaching microscopic slide once that slide has been treated in liquid nitrogen prior sectioning. Dipping of factory-coated microscope slides (Silane-Prep, Sigma) in liquid nitrogen and holding (with forceps) the slide <10 sec (until bubbling will stop) appear to take away most of annoying static charges from otherwise good slides from Sigma. After dipping, slides should be quickly placed inside of the Cryostat (Leica) and used for collecting sections. *Our slides are used for LCM or in situ hybridization. This "super-conductivity magic" did not seem to affect tissue adhesion and it is RNAse-free... Viktor Kharazia & Francesco Giorgetti, Gallo Center, UCSF; kharazia@egcrc.net From ravishankar.nagarajan <@t> ranbaxy.com Mon Mar 21 21:45:12 2005 From: ravishankar.nagarajan <@t> ranbaxy.com (Ravishankar Nagarajan) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Mouse histology books Message-ID: <2533F91DF0FC3F48AE4919B3197765ED2E9433@GUR-PR-EXA1.Ranbaxy.com> Hi Tim, This is for your information. Atlas of Mouse Development Genetic Variants and Strains of the Laboratory Mouse Histological Atlas of the Laboratory Mouse Mouse Histopathology Pathobiology of the Aging Mouse Pathology of Genetically Engineered Mice Pathology of the Mouse: Reference and AtlasAtlas of Mouse Development Genetic Variants and Strains of the Laboratory Mouse All these books are for mice. Atlas of Tumor Pathology of the Fischer Rat Pathobiology of the Aging Rat Pathology of the Fischer Rat Postmortem Change in the Rat Rat Histopathology And these books for rats. All the best for a good reading. Regards, Dr.N.Ravishankar -----Original Message----- From: Timothy Macatee [mailto:timothy.macatee@med.nyu.edu] Sent: Saturday, March 19, 2005 3:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse histology books Hello everyone, Long time reader, first time writer. Since there has been a discussion about books, I was wondering if there is a good adult mouse histology book out there. This way I can see what things are 'suppose' to look like, and then I can test my abilities to orient and embed and cut and stain and ... Thanks Tim Macatee NYU Medical Center -- (i) The information contained in this e-mail message is intended only for the confidential use of the recipient(s) named above. This message is privileged and confidential. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. (ii) The sender confirms that Ranbaxy shall not be responsible if this email message is used for any indecent, unsolicited or illegal purposes, which are in violation of any existing laws and the same shall solely be the responsibility of the sender and that Ranbaxy shall at all times be indemnified of any civil and/ or criminal liabilities or consequences there. From mab70 <@t> medschl.cam.ac.uk Tue Mar 22 02:38:14 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Mouse histology books Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114B7@mius2.medlan.cam.ac.uk> Publishers' details or ISBN references for these books would be very useful if you have time. Thamks Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Ravishankar Nagarajan [mailto:ravishankar.nagarajan@ranbaxy.com] Sent: Tuesday, March 22, 2005 3:45 AM To: Timothy Macatee Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mouse histology books Hi Tim, This is for your information. Atlas of Mouse Development Genetic Variants and Strains of the Laboratory Mouse Histological Atlas of the Laboratory Mouse Mouse Histopathology Pathobiology of the Aging Mouse Pathology of Genetically Engineered Mice Pathology of the Mouse: Reference and AtlasAtlas of Mouse Development Genetic Variants and Strains of the Laboratory Mouse All these books are for mice. Atlas of Tumor Pathology of the Fischer Rat Pathobiology of the Aging Rat Pathology of the Fischer Rat Postmortem Change in the Rat Rat Histopathology And these books for rats. All the best for a good reading. Regards, Dr.N.Ravishankar -----Original Message----- From: Timothy Macatee [mailto:timothy.macatee@med.nyu.edu] Sent: Saturday, March 19, 2005 3:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse histology books Hello everyone, Long time reader, first time writer. Since there has been a discussion about books, I was wondering if there is a good adult mouse histology book out there. This way I can see what things are 'suppose' to look like, and then I can test my abilities to orient and embed and cut and stain and ... Thanks Tim Macatee NYU Medical Center -- (i) The information contained in this e-mail message is intended only for the confidential use of the recipient(s) named above. This message is privileged and confidential. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. (ii) The sender confirms that Ranbaxy shall not be responsible if this email message is used for any indecent, unsolicited or illegal purposes, which are in violation of any existing laws and the same shall solely be the responsibility of the sender and that Ranbaxy shall at all times be indemnified of any civil and/ or criminal liabilities or consequences there. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tanni.Ahmed <@t> intervet.com Tue Mar 22 02:45:00 2005 From: Tanni.Ahmed <@t> intervet.com (Ahmed, T (Tanni)) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] decalcification of avian hock joints Message-ID: <09E7875E806DFB4BBECBADBF966BDBB6917D92@mksn79.d50.intra> Dear Histonetters, I am looking for a workable decalcification procedure for decalcifying samples of avian hock joints (fixed with 10% buffered formalin). I have used Surgipath's decal 1 solution- placing the embedded blocks on soaked tissue paper for 30 mins..is this the normal length of time? Any comments/suggestions would be gratefully appreciated. Thanks everso, Tanni Tanni S Ahmed Histopathology, R&D Intervet UK Ltd. Walton Manor, Walton, Milton Keynes, Buckinghamshire, UK E-mail: tanni.ahmed@intervet.com -------------------------------------- This message, including attachments, is confidential and may be privileged. If you are not an intended recipient, please notify the sender then delete and destroy the original message and all copies. You should not copy, forward and/or disclose this message, in whole or in part, without permission of the sender. -------------------------------------- From ckeith71 <@t> hotmail.com Tue Mar 22 05:22:19 2005 From: ckeith71 <@t> hotmail.com (cindy keith) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] microwave processing Message-ID: Can anyone give me some benefits to microwave processing? Good and bad experiences? Thank You From Rachael_Emerson <@t> URMC.Rochester.edu Tue Mar 22 07:55:10 2005 From: Rachael_Emerson <@t> URMC.Rochester.edu (Emerson, Rachael) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] vectashield Message-ID: Hello, I am using Vectashield Mounting Media which does not seem to harden. Any suggestions on how to seal the slides for long term storage? Thanks Rachael Emerson From Virginia.Ross <@t> nrc-cnrc.gc.ca Tue Mar 22 10:06:59 2005 From: Virginia.Ross <@t> nrc-cnrc.gc.ca (Ross, Virginia) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] RE: Histonet Digest, Vol 16, Issue 35 Message-ID: <10C94843061E094A98C02EB77CFC32870A73DF1E@nrcmrdex1d.imsb.nrc.ca> Somethings wrong with this one. I was trying to read the one you list as "8" about gloves, Glen Dawson and could only find one from the previous mailing from Sharon Osbourne. 8 is about Mouse. Virginia From vazquezr <@t> ohsu.edu Tue Mar 22 10:07:00 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Gloves ... a question Message-ID: I have never worn gloves and I have never had a pathologist complain of skin cells on my slide either. Robyn OHSU >>> "Terry Murphy" 03/18/05 6:50 PM >>> I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? Terry Murphy HTL(ASCP) >From: "Angela Bitting" >To: , >Subject: Re: [Histonet] Gloves ... a question >Date: Fri, 18 Mar 2005 12:16:38 -0500 > >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. >I intentionally did not mention which side of the war I was on because I >wanted evryone to respond without feeling threatened (Histonet is such a >dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > >>> Paul Bradbury 03/18/05 10:31 AM >>> > >From your e-mail I am not sure if you are you waging a battle for or >against the wearing of gloves during embedding and cutting? > >In all the labs I have ever worked in ( and that's quite a few), nobody >has ever worn gloves for embedding/cutting. I don't see what the benefit > >would be. > >Paul > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >IMPORTANT WARNING: The information in this message (and the documents >attached to it, if any) is confidential and may be legally privileged. It >is intended solely for the addressee. Access to this message by anyone else >is unauthorized. If you are not the intended recipient, any disclosure, >copying, distribution or any action taken, or omitted to be taken, in >reliance on it is prohibited and may be unlawful. If you have received this >message in error, please delete all electronic copies of this message (and >the documents attached to it, if any), destroy any hard copies you may have >created and notify me immediately by replying to this email. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ On the road to retirement? Check out MSN Life Events for advice on how to get there! http://lifeevents.msn.com/category.aspx?cid=Retirement _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Tue Mar 22 10:22:29 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Gloves ... a question Message-ID: Cornfield cells can come from a variety of places, including the initial slide labeling. Also, they sometimes fall from your hair onto a slide. Even simply picking up a previously cut slide can sometimes leave cornfield cells. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Tuesday, March 22, 2005 11:07 AM To: akbitting@geisinger.edu; lubbockcat@hotmail.com; histonet@pathology.swmed.edu; histology.bc@shaw.ca Subject: Re: [Histonet] Gloves ... a question I have never worn gloves and I have never had a pathologist complain of skin cells on my slide either. Robyn OHSU >>> "Terry Murphy" 03/18/05 6:50 PM >>> I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? Terry Murphy HTL(ASCP) >From: "Angela Bitting" >To: , >Subject: Re: [Histonet] Gloves ... a question >Date: Fri, 18 Mar 2005 12:16:38 -0500 > >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. I intentionally did not mention >which side of the war I was on because I wanted evryone to respond >without feeling threatened (Histonet is such a dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > >>> Paul Bradbury 03/18/05 10:31 AM >>> > >From your e-mail I am not sure if you are you waging a battle for or >against the wearing of gloves during embedding and cutting? > >In all the labs I have ever worked in ( and that's quite a few), nobody >has ever worn gloves for embedding/cutting. I don't see what the >benefit > >would be. > >Paul > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >IMPORTANT WARNING: The information in this message (and the documents >attached to it, if any) is confidential and may be legally privileged. It >is intended solely for the addressee. Access to this message by anyone else >is unauthorized. If you are not the intended recipient, any disclosure, >copying, distribution or any action taken, or omitted to be taken, in >reliance on it is prohibited and may be unlawful. If you have received this >message in error, please delete all electronic copies of this message (and >the documents attached to it, if any), destroy any hard copies you may have >created and notify me immediately by replying to this email. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ On the road to retirement? Check out MSN Life Events for advice on how to get there! http://lifeevents.msn.com/category.aspx?cid=Retirement _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Mar 22 10:40:29 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] vectashield In-Reply-To: References: Message-ID: <6.0.0.22.1.20050322093133.01b5ba20@gemini.msu.montana.edu> Buy VectaShield Hard Set instead. Otherwise you can seal the edges with diluted permanent mounting media. Xylene and toluene based mounting medias diluted to the consistency of thin nail polish is not miscible with aqueous mounting medias. We avoid nail polish containing isopropyl alcohol as the alcohol can leach under a slide and cause fading of some fluorescent molecules i.e. GFP. Unfortunately, we still store sealed slides flat. At 06:55 AM 3/22/2005, you wrote: >Hello, I am using Vectashield Mounting Media which does not seem to harden. >Any suggestions on how to seal the slides for long term storage? > >Thanks >Rachael Emerson > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From akbitting <@t> geisinger.edu Tue Mar 22 10:42:50 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Ventana/ CoPath interface Message-ID: Has anyone heard anything about how Ventana is coming along with having the capability to interface with Cerner's CoPath system? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From akbitting <@t> geisinger.edu Tue Mar 22 10:44:42 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] DIF on BenchmarkXT Message-ID: Are folks out there doing direct immunofluorescent staining with the BenchmarkXT? And how do you like the results? Are you using your own concentrated antibodies or do you use Ventana predilutes? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From JEllin <@t> yumaregional.org Tue Mar 22 11:02:42 2005 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Ventana/ CoPath interface Message-ID: >From what I understand that they already have an interface with the Tamtron PowerPath system. Jesus Ellin >>> "Angela Bitting" 03/22/05 09:42AM >>> Has anyone heard anything about how Ventana is coming along with having the capability to interface with Cerner's CoPath system? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Tue Mar 22 11:36:35 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Gloves ... a question Message-ID: Hah! That was my spell-check "correcting" something that wasn't incorrect. I typed "cornified" and I guess my auto-check changed it to "cornfield". I will make sure it doesn't happen with this email...I hope! -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Tuesday, March 22, 2005 11:51 AM To: Bartlett, Jeanine Subject: RE: [Histonet] Gloves ... a question origin of "cornfield cells"???, we call them "squams" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bartlett, Jeanine Sent: 22 March 2005 16:22 To: Robyn Vazquez; akbitting@geisinger.edu; lubbockcat@hotmail.com; histonet@pathology.swmed.edu; histology.bc@shaw.ca Subject: RE: [Histonet] Gloves ... a question Cornfield cells can come from a variety of places, including the initial slide labeling. Also, they sometimes fall from your hair onto a slide. Even simply picking up a previously cut slide can sometimes leave cornfield cells. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Tuesday, March 22, 2005 11:07 AM To: akbitting@geisinger.edu; lubbockcat@hotmail.com; histonet@pathology.swmed.edu; histology.bc@shaw.ca Subject: Re: [Histonet] Gloves ... a question I have never worn gloves and I have never had a pathologist complain of skin cells on my slide either. Robyn OHSU >>> "Terry Murphy" 03/18/05 6:50 PM >>> I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? Terry Murphy HTL(ASCP) >From: "Angela Bitting" >To: , >Subject: Re: [Histonet] Gloves ... a question >Date: Fri, 18 Mar 2005 12:16:38 -0500 > >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. I intentionally did not mention >which side of the war I was on because I wanted evryone to respond >without feeling threatened (Histonet is such a dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > >>> Paul Bradbury 03/18/05 10:31 AM >>> > >From your e-mail I am not sure if you are you waging a battle for or >against the wearing of gloves during embedding and cutting? > >In all the labs I have ever worked in ( and that's quite a few), nobody >has ever worn gloves for embedding/cutting. I don't see what the >benefit > >would be. > >Paul > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >IMPORTANT WARNING: The information in this message (and the documents >attached to it, if any) is confidential and may be legally privileged. It >is intended solely for the addressee. Access to this message by anyone else >is unauthorized. If you are not the intended recipient, any disclosure, >copying, distribution or any action taken, or omitted to be taken, in >reliance on it is prohibited and may be unlawful. If you have received this >message in error, please delete all electronic copies of this message (and >the documents attached to it, if any), destroy any hard copies you may have >created and notify me immediately by replying to this email. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ On the road to retirement? Check out MSN Life Events for advice on how to get there! http://lifeevents.msn.com/category.aspx?cid=Retirement _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Tue Mar 22 11:58:47 2005 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Ventana stainer, Symphony Message-ID: <000001c52f08$cc3beb90$1d2a14ac@wchsys.org> The Ventana Symphony can stain at the same time, non-gyn,paps and H&E slides. The stainer squirts the stains onto each individual slide instead of diping into a solution. This provides the ability to prevent cross contamination between Histo & Cyto slides and between non-gyn & paps. Of course robotics and programming are the expensive part. Question: I really appreciate everyone's help so far, it has made our research on automating our lab much more efficient. I began looking at several H&E stainers and don't quit understand Ventana's Symphony product. It is very expensive ($150k) and I'm not sure why it is so much more than all the other comparable products out there. Am I missing something? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 22 12:09:48 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Ventana stainer, Symphony Message-ID: $150K for a machine that does something better done by hand? I think not. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Joyce Cline [mailto:jcline@wchsys.org] Sent: 22 March 2005 17:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana stainer, Symphony The Ventana Symphony can stain at the same time, non-gyn,paps and H&E slides. The stainer squirts the stains onto each individual slide instead of diping into a solution. This provides the ability to prevent cross contamination between Histo & Cyto slides and between non-gyn & paps. Of course robotics and programming are the expensive part. Question: I really appreciate everyone's help so far, it has made our research on automating our lab much more efficient. I began looking at several H&E stainers and don't quit understand Ventana's Symphony product. It is very expensive ($150k) and I'm not sure why it is so much more than all the other comparable products out there. Am I missing something? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Tue Mar 22 12:47:28 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] antibody Message-ID: Does anyone know where to purchase a Cytokeratin for wide Spectrum screening that is a polyclonal antibody? Thanks ahead of time for your responses! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Dorothy.L.Webb <@t> HealthPartners.Com Tue Mar 22 13:08:23 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] antibody Message-ID: Does anyone know where we can purchase a Cytokeratin wide spectrum polyclonal antibody? Thanks ahead of time for the help!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From liz <@t> premierlab.com Tue Mar 22 13:21:09 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] antibody In-Reply-To: Message-ID: <000201c52f14$4dd2f710$76d48a80@AMY> Dako has a wide spectrum screening keratin that is a polycolonal its catalog number is Z0622. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy.L.Webb@HealthPartners.Com Sent: Tuesday, March 22, 2005 11:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody Does anyone know where to purchase a Cytokeratin for wide Spectrum screening that is a polyclonal antibody? Thanks ahead of time for your responses! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Tue Mar 22 14:18:36 2005 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] (no subject) Message-ID: <63B8B599DE283148B92E83C78B32C15D8B49C9@cmhexbe2.childrensmemorial.org> Hello, all, I am going to do Immunostaining of Frozen Sections . I have some questions: 1. What to do with slides after remove them from --80 o C freezer? 2. How long the slides have to come to room temperature? 3. How the OCT compound has to be dissolved? 4. What is general protocol for frozen sections? 5. Am I have to apply Avidin/biotin? My appreciation for any suggestion, Naira Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. From marktarango <@t> earthlink.net Tue Mar 22 14:52:22 2005 From: marktarango <@t> earthlink.net (Mark Adam Tarango) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] H&E slides for HT practical exam Message-ID: <28509852.1111524742645.JavaMail.root@thecount.psp.pas.earthlink.net> Hi everyone. I have a co-worker who insists that she must stain all her H&E and slides for her practical exam together at once. My supervisor and I haven't ever heard this, and I'm hoping someone can give a reply as to whether or not this is something that is required. She is conviced that if she doesn't stain them all together (her H&E slides), someone will know and fail her again. If Peggy Wenk or Jennifer MacDonald are watching, we'd like to her from you especially. Thanks, Mark Tarango From marktarango <@t> earthlink.net Tue Mar 22 15:19:06 2005 From: marktarango <@t> earthlink.net (Mark Adam Tarango) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] H&E slides for HT practical exam Message-ID: <2864801.1111526346689.JavaMail.root@gonzo.psp.pas.earthlink.net> Hi everyone. I have a co-worker who insists that she must stain all her H&E and slides for her practical exam together at once. My supervisor and I haven't ever heard this, and I'm hoping someone can give a reply as to whether or not this is something that is required. She is conviced that if she doesn't stain them all together (her H&E slides), someone will know and fail her again. If Peggy Wenk or Jennifer MacDonald are watching, we'd like to her from you especially. Thanks, Mark Tarango From kbradshaw <@t> lcpath.com Tue Mar 22 15:01:29 2005 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Ventana Benchmark HPV In-Reply-To: <813FB33DA405334F947F8BFC6EBD0B2AE69E@bbplsrv1.bbpl> Message-ID: WE have experienced the same problem from time to time. There is a shot glass size reservoir within the Benchmark where there is solution(buffer) exchange. Sometimes depending on what type of run you have been doing (ISH vs. IHC) there can be a pH problem. To remedy this you can run 2 different function tests. 1. SSC purge, empties the shot glass 2. EX prep purge, corrects pH If you run these two tests before your HPV run you should eradicate your "blue Haze" problems. :) Kari Bradshaw, HT(ASCP) Laboratory Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 (360)425-5620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pat Zeitlow Sent: Friday, March 18, 2005 12:06 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Ventana Benchmark HPV Has anyone doing HPV ISH on Benchmark (not XT) experienced background problems on slides that prohibits evaluation? We are seeing random problems with this "Blue haze" and cannot seem to get it resolved. I realize I haven't provided much detail, but if anyone is having this same issue, they will recognize my description. Thanks in advance for any input! Pat Z Department of Molecular Pathology Boyce and Bynum Pathology Labs, P.C. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Wed Mar 23 01:33:45 2005 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re gloves and squames Message-ID: <000801c52f7a$a560d770$4900fe0a@Carlos> I recall a Pathologist demanding that I cut my hair( many moons ago when I had long hair) because he insisted that it was shedding squames onto his sections! Interestingly, the two females in the lab, both of whom had equally long hair, in a similar style, were not commmanded to do the same.......needless to say, I refused. I put a glove over my head instead..... Only kidding. Washing my hands before section cutting and careful handling always minimised squames' shedding onto sections, for me. From mab70 <@t> medschl.cam.ac.uk Wed Mar 23 02:01:01 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] vectashield Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114B8@mius2.medlan.cam.ac.uk> Nail varnish is the traditional way, but we use a small paint brush dipped in our normal permanent mountant, e.g. DPX, XAM or whatever your usual one is. It works just as well but isn't so colourful! Regards Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Emerson, Rachael [mailto:Rachael_Emerson@URMC.Rochester.edu] Sent: Tuesday, March 22, 2005 1:55 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] vectashield Hello, I am using Vectashield Mounting Media which does not seem to harden. Any suggestions on how to seal the slides for long term storage? Thanks Rachael Emerson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Wed Mar 23 02:05:42 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] vectashield Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114B9@mius2.medlan.cam.ac.uk> I campaigned for Vectashield hard set for years as their rep will witness. I have now tried it, but find it forms air bubbles on storing, has anyone found the answer to that problem? I know you can remount the slides, but this is an additional chore. Otherwise I just stick to the old version. Margaret -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Tuesday, March 22, 2005 4:40 PM To: Emerson, Rachael; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] vectashield Buy VectaShield Hard Set instead. Otherwise you can seal the edges with diluted permanent mounting media. Xylene and toluene based mounting medias diluted to the consistency of thin nail polish is not miscible with aqueous mounting medias. We avoid nail polish containing isopropyl alcohol as the alcohol can leach under a slide and cause fading of some fluorescent molecules i.e. GFP. Unfortunately, we still store sealed slides flat. At 06:55 AM 3/22/2005, you wrote: >Hello, I am using Vectashield Mounting Media which does not seem to harden. >Any suggestions on how to seal the slides for long term storage? > >Thanks >Rachael Emerson > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Wed Mar 23 02:44:21 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] (no subject) Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114BA@mius2.medlan.cam.ac.uk> Dear Naira, You should air dry your sections for about 1 hour. The OCT will wash off in the wash buffers. Whether you need to do an avidin/biotin block will depend upon the tissue, but you should try with and without to test this. You may need to experiment with fixation, look at the datasheet for the antibody and follow the recommendations. You may need to use paraformaldehyde fixation or even plp for which you should be able to find the formula in a good textbook. I use Tris buffered saline (TBS) for washing, others prefer phosphate buffered saline (PBS). You will find a good protocol in the pack insert in any of Vector's ABC kits, which are excellent. these pack inserts also have a trouble shooting guide to help you. I hope this helps. If you want a copy of my protocol, please contact me. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Margaryan, Naira [mailto:NMargaryan@childrensmemorial.org] Sent: Tuesday, March 22, 2005 8:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello, all, I am going to do Immunostaining of Frozen Sections . I have some questions: 1. What to do with slides after remove them from --80 o C freezer? 2. How long the slides have to come to room temperature? 3. How the OCT compound has to be dissolved? 4. What is general protocol for frozen sections? 5. Am I have to apply Avidin/biotin? My appreciation for any suggestion, Naira Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed Mar 23 03:55:25 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] H&E slides for HT practical exam References: <28509852.1111524742645.JavaMail.root@thecount.psp.pas.earthlink.net> Message-ID: <009601c52f8e$70240f00$04f4ff44@domainnotset.invalid> Slides can be stained all together or one at a time. There is no way anyone can tell. In fact, time in each solution can be different for each tissue stained. What is important is that EACH slide be well stained - good crisp nuclei with chromatin pattern, a minimum of three shades of eosin that does not overwhelm the hematoxylin, etc.. If that takes taking each slide through the different solutions/stains at different time intervals, repeating and tweaking the stain 20 times with different time intervals from what you normally do, and changing to different times for each block depending upon the tissue/length of time it has been fixed/tissue thickness/etc. - then do it. It is more much more important to know what to do to produce a well stained slide, than to rigidly adhere to the same steps/times. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Mark Adam Tarango" To: Sent: Tuesday, March 22, 2005 3:52 PM Subject: [Histonet] H&E slides for HT practical exam > Hi everyone. I have a co-worker who insists that she must stain all her H&E and slides for her practical exam together at once. My supervisor and I haven't ever heard this, and I'm hoping someone can give a reply as to whether or not this is something that is required. She is conviced that if she doesn't stain them all together (her H&E slides), someone will know and fail her again. If Peggy Wenk or Jennifer MacDonald are watching, we'd like to her from you especially. > > > Thanks, > > > Mark Tarango > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Mar 23 06:24:23 2005 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:24:48 2005 Subject: AW: [Histonet] DIF on BenchmarkXT In-Reply-To: Message-ID: I am also interested in this technique on the Benchmark XT. Would you be so kind and share the answers with me? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Angela Bitting Gesendet: Dienstag, 22. M?rz 2005 17:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] DIF on BenchmarkXT Are folks out there doing direct immunofluorescent staining with the BenchmarkXT? And how do you like the results? Are you using your own concentrated antibodies or do you use Ventana predilutes? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jengirl1014 <@t> yahoo.com Wed Mar 23 07:14:59 2005 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] RE: vectashield Message-ID: <20050323131459.4710.qmail@web60602.mail.yahoo.com> I use Permount and have no problems using it. I'd reccommend that. Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From TillRenee <@t> uams.edu Wed Mar 23 07:49:56 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] PCNA on cryosection Message-ID: Can anyone give me a general protocol for what to do with cryosections that will be used for the PCNA stain? I have the PCNA kit from Zymed. They say you can use them on frozens, but that is about all the help they give. I know I asked this question before, but this time I actually have a specific stain in mind. One of my other Zymed kits says to fix in acetone, so I thought I might do that. Should you fix after cutting, or wait until you are going to use the slides? If you are going to wait I would think the slides should be kept in -80? Any suggestions? Renee' Till, HT Arkansas Childrens Nutrition Center 1120 Marshall Little Rock, AR 72202 (501) 364-2774 From kelly.mcqueeney <@t> bms.com Wed Mar 23 08:06:23 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] BrdU protocol on frozen brain tissue Message-ID: <424177DF.6040608@bms.com> Does anyone have a protocol for BrdU IHC on frozen brain tissue? Thanks, Kelly McQueeney From mab70 <@t> medschl.cam.ac.uk Wed Mar 23 08:08:49 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] PCNA on cryosection Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114BE@mius2.medlan.cam.ac.uk> Seal them in an airtight microscope box or wrap them carefully in cling film and store at -80. On thawing allow them to equilibrate at room temperature on a staining tray for about an hour. Then fix in acetone for about 10 minutes, I usually use acetone at room temperature, but it can be used at -20C. If you do the latter, make sure that the equipment you use is spark proof! I haven't done PCNA on frozens so can't give you a protocol, but I would use the protocol you normally use for frozens. Reading your email again, it looks as though the acetone fixation is for another antibody, have Zymed not provided a datasheet for the PCNA kit? If not, you should either visit the website and download the appropriate datasheet or ring them and ask for it. That is the whole point of a kit - it comes with instructions and is optimised, otherwise why pay extra for it?The individual reagents are much cheaper on their own as a rule. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Till, Renee [mailto:TillRenee@uams.edu] Sent: Wednesday, March 23, 2005 1:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PCNA on cryosection Can anyone give me a general protocol for what to do with cryosections that will be used for the PCNA stain? I have the PCNA kit from Zymed. They say you can use them on frozens, but that is about all the help they give. I know I asked this question before, but this time I actually have a specific stain in mind. One of my other Zymed kits says to fix in acetone, so I thought I might do that. Should you fix after cutting, or wait until you are going to use the slides? If you are going to wait I would think the slides should be kept in -80? Any suggestions? Renee' Till, HT Arkansas Childrens Nutrition Center 1120 Marshall Little Rock, AR 72202 (501) 364-2774 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Wed Mar 23 08:19:39 2005 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] H&E slides for HT practical exam In-Reply-To: <009601c52f8e$70240f00$04f4ff44@domainnotset.invalid> References: <28509852.1111524742645.JavaMail.root@thecount.psp.pas.earthlink.net> <009601c52f8e$70240f00$04f4ff44@domainnotset.invalid> Message-ID: <1111587579.42417afbcb4f2@imp.vet.upenn.edu> I agree with Peggy. It is better to learn to be flexible and do excellent work than adhere to policy that only produces a ridgid stain regime. The goal is to produce slides that are of the highest quality for each tissue and stain. Unforutnately we have seen and heard from people taking the test for ASCP license in Histology who cut the same blocks and stianed the slides at the same time when one passed and one failed. I have yet to see anyone on the grading committee address this issue. Sorry to open this can of worms again however, when I took my test in the "70s I was told not to submit any animal tissue (I was doing research on animals only) or I would fail. I know that has changed and I did find a way (pre-HIPPA) to get my tissue and stain but it shows the differences in how we are treated and graded over the years. Other than what you stated for H&E is there a true standard for each stain or does it depend on the person grading and what they like or see. Pam Marcum Quoting lpwenk@sbcglobal.net: > Slides can be stained all together or one at a time. There is no way anyone > can tell. > > In fact, time in each solution can be different for each tissue stained. > > What is important is that EACH slide be well stained - good crisp nuclei > with chromatin pattern, a minimum of three shades of eosin that does not > overwhelm the hematoxylin, etc.. > > If that takes taking each slide through the different solutions/stains at > different time intervals, repeating and tweaking the stain 20 times with > different time intervals from what you normally do, and changing to > different times for each block depending upon the tissue/length of time it > has been fixed/tissue thickness/etc. - then do it. It is more much more > important to know what to do to produce a well stained slide, than to > rigidly adhere to the same steps/times. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > ----- Original Message ----- > From: "Mark Adam Tarango" > To: > Sent: Tuesday, March 22, 2005 3:52 PM > Subject: [Histonet] H&E slides for HT practical exam > > > > Hi everyone. I have a co-worker who insists that she must stain all her > H&E and slides for her practical exam together at once. My supervisor and I > haven't ever heard this, and I'm hoping someone can give a reply as to > whether or not this is something that is required. She is conviced that if > she doesn't stain them all together (her H&E slides), someone will know and > fail her again. If Peggy Wenk or Jennifer MacDonald are watching, we'd > like to her from you especially. > > > > > > Thanks, > > > > > > Mark Tarango > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.j.savage <@t> gsk.com Wed Mar 23 09:27:55 2005 From: tony.j.savage <@t> gsk.com (tony.j.savage@gsk.com) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re:Gloves ..... a question In-Reply-To: <200503221758.j2MHw52U024349@rtpsfimr001.glaxo.com> Message-ID: I also have never worn gloves for embedding or cutting paraffin processed material although I can see the merit of wearing gloves when cutting unfixed frozen material. I was also intrigued by the comments about dead skin cells occurring on slides. It is highly unlikely that you would ever identify the fragments of keratin that might be shed from your hands when cutting sections; you just would not get cornified epithelium shed from the hands or scalp because these sites are heavily keratinised. You are much more likely to get the cornified cells deposited on slides from the mouth, by breathing on the block whilst cutting or onto the slide whilst cleaning. I have also noticed that there are times when deposited cornified cells increase to problem levels and this often coincides with the microtomist having a cold or a sore throat. I strongly feel that the notion of cornified cells from the hand/scalp is a myth that is passed from generation to generation often by practitioners who should have given it more thought. >>>>"Robyn Vazquez" Subject: Re: [Histonet] Gloves ... a question I have never worn gloves and I have never had a pathologist complain of skin cells on my slide either. Robyn OHSU >>> "Terry Murphy" 03/18/05 6:50 PM >>> I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? >>> "Angela Bitting" >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. >I intentionally did not mention which side of the war I was on because I >wanted evryone to respond without feeling threatened (Histonet is such a >dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm From JQB7 <@t> CDC.GOV Wed Mar 23 09:38:21 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re:Gloves ..... a question Message-ID: There was a gentleman that a friend of mine worked with for years that always had many more problems with this than any other in the group. They determined it was due to him excessively scratching his head near the waterbath. Once they brought it to his attention and he stopped, so did the problem. Go figure. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tony.j.savage@gsk.com Sent: Wednesday, March 23, 2005 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:Gloves ..... a question I also have never worn gloves for embedding or cutting paraffin processed material although I can see the merit of wearing gloves when cutting unfixed frozen material. I was also intrigued by the comments about dead skin cells occurring on slides. It is highly unlikely that you would ever identify the fragments of keratin that might be shed from your hands when cutting sections; you just would not get cornified epithelium shed from the hands or scalp because these sites are heavily keratinised. You are much more likely to get the cornified cells deposited on slides from the mouth, by breathing on the block whilst cutting or onto the slide whilst cleaning. I have also noticed that there are times when deposited cornified cells increase to problem levels and this often coincides with the microtomist having a cold or a sore throat. I strongly feel that the notion of cornified cells from the hand/scalp is a myth that is passed from generation to generation often by practitioners who should have given it more thought. >>>>"Robyn Vazquez" Subject: Re: [Histonet] Gloves ... a question I have never worn gloves and I have never had a pathologist complain of skin cells on my slide either. Robyn OHSU >>> "Terry Murphy" 03/18/05 6:50 PM >>> I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? >>> "Angela Bitting" >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. I intentionally did not mention >which side of the war I was on because I wanted evryone to respond >without feeling threatened (Histonet is such a dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcrippen <@t> buckinstitute.org Wed Mar 23 09:46:23 2005 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] BrdU protocol on frozen brain tissue Message-ID: Dear Kelly, We've had great success with the following protocol using the monoclonal BrdU Ab from Roche (1:100) on mouse brain tissue(20um sections)and Vector's Avidin/Biotin blocking kit to block endogenous biotin: Immunocytochemical detection of BrdU-labled nuclei 1. Air dry samples for 20min. 2. cold MetOH fix for 20min @ -20C, air dry for 10min 3. Then incubated at 37C (slide moat) for 30min in 2N HCl .. 4. Sections are rinsed for 10 min at room temp in 0.1 M boric acid pH 8.5. 5. Incubate the sections for 10 min 1x PBS. 6. 15 min in 3% H2O2 in dH2O or MetOH 7. wash in PBS 5 min 8. Block in (1x PBS, 1% NHS, .1% BSA, .3% Triton) +Chicken anti MsIgG (2ug/ml) for 30 mins at RT 9. Sections are then incubated overnight at 4?C in primary antibody diluted in block buffer (PBS with 1% horse serum, 0.1% bovine serum albumin, and 0.3% Triton X-100) at its optimal concentration. 10. After being washed in PBS 3x5min, sections are incubated in biotinylated secondary antibody (Vector)diluted in block buffer for 2 hr at room temperature. 11. Make VECTASTAIN Elite ABC Reagent and allow VECTASTAIN Elite ABC Reagent to stand for about 30 minutes before use. 12. After three 5 min rinses in PBS. 13. Incubate sections for 30 minutes with VECTASTAIN ABC Reagent . 14. Wash slides for 5 minutes in PBS. 15. Incubate sections in peroxidase substrate solution (we use DAB kit from Vector) until desired stain intensity develops. 16. Rinse sections in tap water. 17. Dehydrate sections with 75%, 95%, 100% ethanol (3 min each) and Xylene (5 min). 18. Mount the sections with permanent mounting medium. Danielle Crippen Morphology Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kelly D Mcqueeney Sent: Wednesday, March 23, 2005 6:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BrdU protocol on frozen brain tissue Does anyone have a protocol for BrdU IHC on frozen brain tissue? Thanks, Kelly McQueeney _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Mar 23 10:23:02 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:48 2005 Subject: [Histonet] Re:Gloves ..... a question Message-ID: This post is difficult to understand. What on earth are cornified (I liked cornfield better) cells other than keratinised cells? Where would you most likely get lots of keratinised cells from, a lightly keratinised site or a heavily keratinised site? The mouth is, for the most part non-keratinised, and is therefore not in contention at all as a site of origin. Let me confirm that it is sometimes a problem. I feel sure that there are either shedders and non-shedders, that there are someday shedders and other day non-shedders (if you see what I mean). When I was in NZ a few years ago, it was a really bad problem, solved by getting the tech to wear gloves when labelling the slides. Slides are invariably labelled with the writing right way up, frosted end pointing North. This means the fingers and heel of the hand rest on the slide. That is the source of the squames. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: tony.j.savage@gsk.com [mailto:tony.j.savage@gsk.com] Sent: 23 March 2005 15:28 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:Gloves ..... a question I also have never worn gloves for embedding or cutting paraffin processed material although I can see the merit of wearing gloves when cutting unfixed frozen material. I was also intrigued by the comments about dead skin cells occurring on slides. It is highly unlikely that you would ever identify the fragments of keratin that might be shed from your hands when cutting sections; you just would not get cornified epithelium shed from the hands or scalp because these sites are heavily keratinised. You are much more likely to get the cornified cells deposited on slides from the mouth, by breathing on the block whilst cutting or onto the slide whilst cleaning. I have also noticed that there are times when deposited cornified cells increase to problem levels and this often coincides with the microtomist having a cold or a sore throat. I strongly feel that the notion of cornified cells from the hand/scalp is a myth that is passed from generation to generation often by practitioners who should have given it more thought. >>>>"Robyn Vazquez" Subject: Re: [Histonet] Gloves ... a question I have never worn gloves and I have never had a pathologist complain of skin cells on my slide either. Robyn OHSU >>> "Terry Murphy" 03/18/05 6:50 PM >>> I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? >>> "Angela Bitting" >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. >I intentionally did not mention which side of the war I was on because I >wanted evryone to respond without feeling threatened (Histonet is such a >dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Wed Mar 23 10:51:13 2005 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CB45@usca0082k08.labvision.apogent.com> Sounds to me like the makings of a research project for a histotech student! Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Wednesday, March 23, 2005 8:23 AM To: tony.j.savage@gsk.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:Gloves ..... a question This post is difficult to understand. What on earth are cornified (I liked cornfield better) cells other than keratinised cells? Where would you most likely get lots of keratinised cells from, a lightly keratinised site or a heavily keratinised site? The mouth is, for the most part non-keratinised, and is therefore not in contention at all as a site of origin. Let me confirm that it is sometimes a problem. I feel sure that there are either shedders and non-shedders, that there are someday shedders and other day non-shedders (if you see what I mean). When I was in NZ a few years ago, it was a really bad problem, solved by getting the tech to wear gloves when labelling the slides. Slides are invariably labelled with the writing right way up, frosted end pointing North. This means the fingers and heel of the hand rest on the slide. That is the source of the squames. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: tony.j.savage@gsk.com [mailto:tony.j.savage@gsk.com] Sent: 23 March 2005 15:28 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:Gloves ..... a question I also have never worn gloves for embedding or cutting paraffin processed material although I can see the merit of wearing gloves when cutting unfixed frozen material. I was also intrigued by the comments about dead skin cells occurring on slides. It is highly unlikely that you would ever identify the fragments of keratin that might be shed from your hands when cutting sections; you just would not get cornified epithelium shed from the hands or scalp because these sites are heavily keratinised. You are much more likely to get the cornified cells deposited on slides from the mouth, by breathing on the block whilst cutting or onto the slide whilst cleaning. I have also noticed that there are times when deposited cornified cells increase to problem levels and this often coincides with the microtomist having a cold or a sore throat. I strongly feel that the notion of cornified cells from the hand/scalp is a myth that is passed from generation to generation often by practitioners who should have given it more thought. >>>>"Robyn Vazquez" Subject: Re: [Histonet] Gloves ... a question I have never worn gloves and I have never had a pathologist complain of skin cells on my slide either. Robyn OHSU >>> "Terry Murphy" 03/18/05 6:50 PM >>> I once had a pathologist complain dead skin cells on his slides when he saw that I did not wear gloves when I was cutting. Anyone else ever hear this from a pathologist? >>> "Angela Bitting" >Thank you to everyone who replied to my question about wearing gloves >during embedding and cutting blocks. I intentionally did not mention >which side of the war I was on because I wanted evryone to respond >without feeling threatened (Histonet is such a dangerous place)LOL >Thank you again. >By the way, >I'm on the side of those who DO NOT wear gloves..... Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie2507 <@t> netzero.com Wed Mar 23 11:33:33 2005 From: mickie2507 <@t> netzero.com (mickie2507@netzero.com) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Reprocessing tissues Message-ID: <20050323.093426.29075.34303@webmail03.lax.untd.com> Hello Heather, I am behind in my histonet reading but saw your question about reprocessing. I agree, one should expect specimens to be grossed in the correct thickness. Alas, often this does not happen. I wrote an article in the Histo-logic in 2003 which compares reprocessing by the method of putting the block (with the paraffin melted away) through a purge cycle on the processor versus a technic which we used at Sacred Heart Medical Center for a number of years, which is less harsh and just as simple. Much simpler than reprocessing by hand too. One melts the block down and blots off the excess paraffin and then starts the block off on the next nights run in formalin. The rationale being that the tissue in the block which is infiltrated with paraffin will be protected from over-dehydration. The paraffin will disolve out in Xylene and then the whole block will be reinfiltrated with paraffin. In practice the cytological detail is excellent and the cutting properties are also excellent. Try this and see if it works for you. Best Regards, Mickie Mickie Johnosn, B.A., HT/HTL(ASCP) Mohs Histology Temporary Services Training, Consultation & Vacation Relief for Mohs and Clinical Histology. web-site: www.mohshistotemp.com ______________________________________________________________________ Speed up your surfing with NetZero HiSpeed. Now includes pop-up blocker! Only $14.95/month -visit http://www.netzero.com/surf to sign up today! From BennettW <@t> pac.dfo-mpo.gc.ca Wed Mar 23 11:46:19 2005 From: BennettW <@t> pac.dfo-mpo.gc.ca (BennettW@pac.dfo-mpo.gc.ca) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Embedding Media Message-ID: <7CBBD627E4E688499349A5D11D078316020DBBCF@msgpacpbs.rhq.pac.dfo-mpo.gc.ca> Hi All, Does anyone use Surgipath EM 400 paraffin? If so have you noticed any differences between batches in regards to cracking, tissues shattering when cutting, excessive wrinkling etc.??? We tried one order of EM 400 and everything was O.K. But we seem to be having problems with the second batch. Has anyone noticed these type of problems from batch to batch. Does anyone know if Surgipath has QA/QC problems with paraffin? Thanks in advance. Cheers Bill Bennett Histologist Fisheries and Oceans Canada From GoodwinD <@t> pahosp.com Wed Mar 23 12:04:48 2005 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Wright giemsa on bone marrow core biopsies... Message-ID: <992899E9EC268548AB8DDE246AF88473055F4F0A@PAHEX01.uphs.upenn.edu> ...looking for a good method for tissue as opposed to smears. Thanks, Diana Goodwin Anatomic Pathology Pennsylvania Hospital 215-829-6532 e-mail: goodwind@pahosp.com From mickie2507 <@t> netzero.com Wed Mar 23 12:15:02 2005 From: mickie2507 <@t> netzero.com (mickie2507@netzero.com) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Cutting Fat Message-ID: <20050323.101548.5555.34199@webmail25.lax.untd.com> This is a response to an old question about cutting good sections of fat. I am a traveling Mohs histotech and also train Mohs histotechs. With Mohs frozen sections, getting sections of fat is paramount. If there is a hole in the fat, then the Mohs surgeon cannot evaluate that area for residual tumor. Mohs techs work hard to get good sections of skin with no holes in the fat. In my experience, high quality sections of fat can be achieved using liquid nitrogen to cool the fat sufficiently to allow it to cut. One can use a large swab dipped in liquid nitrogen or a device for freezing warts off commonly used in dermatology offices is very handy. I have found that the temperature of the cryostat is not as important as the temperature of the fat being cut. Sometimes freezing several times is needed before the fat 'gels'. At that point, it doesn't take much more freezing to get the fat to cut very nicely. At times it is also helpful to cut the sections thicker, perhaps up to 10 microns. Once the fat 'gels' the micron setting can be turned down some, if thickness is a problem. Use of sharp disposible blades is a must. When I cut fatty blocks of skin from the scalp, back, chest, etc, I routinely get excellent sections of fat. Hope this helps. Mickie Johnosn, B.A., HT/HTL(ASCP) Mohs Histology Temporary Services Training, Consultation & Vacation Relief for Mohs and Clinical Histology. web-site: www.mohshistotemp.com ______________________________________________________________________ Speed up your surfing with NetZero HiSpeed. Now includes pop-up blocker! Only $14.95/month -visit http://www.netzero.com/surf to sign up today! From c.m.vanderloos <@t> amc.uva.nl Wed Mar 23 12:42:12 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] RE: PCNA on cryosection Message-ID: <21768532179b0c.2179b0c2176853@amc.uva.nl> Hi Renee, PCNA isn't working very well on frozen tissue sections. At least not in my hands some time ago. We use the monoclonal anti-ki67 (LabVision/Neomarkers) for human, mouse and rat instead. If you wish to stain nuclear antigens in a cryostat tissue section you better avoid acetone as a fixative as this will result into a quite diffuse staining. My tip for a crisp nuclear staining on frozens is just a 5 minutes fixation with NBF. No antigen retrieval is needed. Hope this helps. Chris van der Loos, PhD Dept. of Pathology M2-230 Academical Medical Center Meibergdreef 9 NL-1105 AZ Amsterdam, The Netherlands ----- Original Message ----- From "Till, Renee" Date Wed, 23 Mar 2005 07:49:56 -0600 To histonet@lists.utsouthwestern.edu Subject [Histonet] PCNA on cryosection Can anyone give me a general protocol for what to do with cryosections that will be used for the PCNA stain? I have the PCNA kit from Zymed. They say you can use them on frozens, but that is about all the help they give. I know I asked this question before, but this time I actually have a specific stain in mind. One of my other Zymed kits says to fix in acetone, so I thought I might do that. Should you fix after cutting, or wait until you are going to use the slides? If you are going to wait I would think the slides should be kept in -80? Any suggestions? Renee' Till, HT Arkansas Childrens Nutrition Center 1120 Marshall Little Rock, AR From c.m.vanderloos <@t> amc.uva.nl Wed Mar 23 12:53:54 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] RE: Dako CSAII vs Elite ABC method, which is more sensitive? Message-ID: <2176fec217c21c.217c21c2176fec@amc.uva.nl> Hi Kelly, When it comes the question which of the two techniques is more sensitive I can predict that very certainly the CSA II kit will beat the Elite ABC method for. It's just a matter of choices what do you prefer and what is technically possible. Personally, I use the CSA II kit as my last resort in case the signal with other detection techniques is too weak: Start using the Elite ABC kit (or EnVision, or other "simple" detection system) and titrate your primary with appropriate pretreatment over a wide range at a proven positive control. If it works fine, you're done. If the signal is too weak, testing with the CSA II kit will be step 2. Again, perform a titration experiment over a wide range at the same positive control tissue. You should also consider that CSA II kit signal is less crisp than with other IHC detection systems. Usually this isn't too disturbing. The dotty appearance of the reaction product is due to the tyramide reaction and can therefore not be improved. Hope this helps. Chris van der Loos, PhD Dept. of Pathology M2-230 Academical Medical Center Meibergdreef 9 NL-1105 AZ Amsterdam, The Netherlands ----- Original Message ----- From Kelly D Mcqueeney Date Mon, 21 Mar 2005 16:24:59 -0500 To histonet@lists.utsouthwestern.edu Subject [Histonet] Dako CSAII vs Elite ABC method, which is more sensitive? I have been using Elite ABC kit for c-fos IHC in brain. We are getting pretty good staining and want to improve sensitivity. I compared the Elite ABC kit, Dako's CSA tyramide amplification kit, and Dako's Envison + kit. The sections are slide-mounted, 20 micron (I increased Dako's incubation times to 45 minutes). Dako claims that the tyramide and Envision kit are much more sensitive than Elite ABC. I have used the tyramide system in the past and was really impressed with the increased signal. Unfortuantely, Dako's CSA and Envision kit exhibit very weak staining and the Elite ABC kit exhibits dark and specific staining in the brain. Has anyone else noticed a change in either product? Should I order HRP-conjugated c-fos and amplify the signal? Any suggestions (besides floating sections)? Method: Quench w/Dako's H2O2, block 1 hour 3% BSA in PBT, c-fos O/N, wash buffer is PBS +! 0.01% Tw From Vickroy.Jim <@t> mhsil.com Wed Mar 23 13:13:10 2005 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] copper stain for liver biopsies Message-ID: We are looking for a procedure for staining for copper. In the past we have used an aldehyde fuchsin stain but our pathologist would like to use a rhodanine stain. Does anybody have a good procedure for staining copper and any suggestions where to get a kit or reagents? Jim Vickroy Memorial Medical Stain Springfield, Illinois From BDUE <@t> PARTNERS.ORG Wed Mar 23 13:32:07 2005 From: BDUE <@t> PARTNERS.ORG (Due, Brice) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Cutting Fat... Thanks! Message-ID: <59C772E2D8EDF345AE1601F5B60B3CB5027966@PHSXMB7.partners.org> Thank You Mickie! I posted earlier my dry ice tricks for cutting fatty human muscles. Wouldn't you know I was just now struggling with a bx laced with veins of fat... I sat down for a moment and saw your post. A big ob-gyn swab soaked in LN2 turned out to be more convenient than several dry ice pellets. A little more difficult to control the temperature, but between my thumb on the bx and the LN2 swab everywhere else, I was able to get the sections I needed. Static seems worse than with dry ice, but I don't mind picking sections up off the roll plate instead of the stage. I'll have to play with this more in the future! Thanks Again! -brice Brigham & Women's Neuropathology Boston, MA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of mickie2507@netzero.com Sent: Wednesday, March 23, 2005 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting Fat This is a response to an old question about cutting good sections of fat. I am a traveling Mohs histotech and also train Mohs histotechs. With Mohs frozen sections, getting sections of fat is paramount. If there is a hole in the fat, then the Mohs surgeon cannot evaluate that area for residual tumor. Mohs techs work hard to get good sections of skin with no holes in the fat. In my experience, high quality sections of fat can be achieved using liquid nitrogen to cool the fat sufficiently to allow it to cut. One can use a large swab dipped in liquid nitrogen or a device for freezing warts off commonly used in dermatology offices is very handy. I have found that the temperature of the cryostat is not as important as the temperature of the fat being cut. Sometimes freezing several times is needed before the fat 'gels'. At that point, it doesn't take much more freezing to get the fat to cut very nicely. At times it is also helpful to cut the sections thicker, perhaps up to 10 microns. Once the fat 'gels' the micron setting can be turned down some, if thickness is a problem. Use of sharp disposible blades is a must. When I cut fatty blocks of skin from the scalp, back, chest, etc, I routinely get excellent sections of fat. Hope this helps. Mickie Johnosn, B.A., HT/HTL(ASCP) Mohs Histology Temporary Services Training, Consultation & Vacation Relief for Mohs and Clinical Histology. web-site: www.mohshistotemp.com ______________________________________________________________________ Speed up your surfing with NetZero HiSpeed. Now includes pop-up blocker! Only $14.95/month -visit http://www.netzero.com/surf to sign up today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sa.drew <@t> hosp.wisc.edu Wed Mar 23 15:37:42 2005 From: sa.drew <@t> hosp.wisc.edu (Drew Sally A.) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Pax-5 Message-ID: Would anyone care to share their experiences with Pax-5 antibody on the Ventana immunostainers? I'm puzzled by my initial negative results using Hodgkin's disease positive lymph nodes and tonsil...Are there any known quirks to this antibody? Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From jkiernan <@t> uwo.ca Wed Mar 23 16:42:14 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] copper stain for liver biopsies References: Message-ID: <4241F0C6.C59FE020@uwo.ca> The reagent is p-dimethylaminobenzylidenerhodanine (pDMBR). Don't buy rhodanine; it's a different compound and it won't work. Also don't confuse with rhodamine and similarly named substances, which are completely unrelated. pDMBR 24 mg 100% ethanol 6 ml Before using, filter this solution into 45 ml of 0.065M sodium acetate (= 1.6% anhydrous, or 2% trihydrate) Put hydrated slides in the working solution either overnight at 37C or 11 min at 80C. Wash X6 in distilled water. Counterstain nuclei lightly with haemalum. Wash. Aqueous mountant. Cu-containing deposits brownish red. Sometimes it's necessary to release "masked" copper before doing the method. Before doing a histochemical method of this kind, read up about its mechanism, limitations and relations to other methods: Pearse's Histochemistry 4th ed Vol 2 (the 2nd or 3rd ed also OK). C. Chrukian's "Manual of the Special Stains Laboratory" 1997 or later edition. Irons et al 1977 Arch Path Lab Med 101,298-301 (This paper compares different methods for Cu.) -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ "Vickroy, Jim" wrote: > > We are looking for a procedure for staining for copper. In the past we > have used an aldehyde fuchsin stain but our pathologist would like to > use a rhodanine stain. Does anybody have a good procedure for staining > copper and any suggestions where to get a kit or reagents? > > Jim Vickroy > > Memorial Medical Stain > > Springfield, Illinois > _______________________________________________ From jkiernan <@t> uwo.ca Wed Mar 23 16:42:59 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question References: Message-ID: <4241F0F3.188652CD@uwo.ca> "Marshall Terry Dr, Consultant Histopathologist" wrote: > ... Slides are invariably labelled with the writing right > way up, frosted end pointing North. This means the fingers > and heel of the hand rest on the slide. That is the > source of the squames. Now that's interesting. I've only ever written on the frosted end with the section end pointing north. Why would anyone want to do it the other way round? -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ From cmconway <@t> usgs.gov Wed Mar 23 16:48:07 2005 From: cmconway <@t> usgs.gov (Carla M Conway) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] problem with melanin staining reddish-brown by AEC chromogen Message-ID: Hello all, I am optimizing my protocol for a new antibody against the nucleocapsid protein of a fish rhabdovirus. Unfortunately, melanin pigment (especially large concentrations of it) is reddish-brown after staining with the AEC chromogen. This occurs on controls and test tissue, regardless of primary antibody dilution. Determining actual positive areas (if any) is problematic. I am using Dako's EnVision+ system and have been pleased with results using other antibodies. Switching to DAB then staining with Azure B is an option (this system uses either AEC or DAB), but I prefer to stay with AEC if at all possible. Comparing an H&E stained slide to its IHC counterpart is time consuming, but helpful. I have thought about reducing the chromogen incubation time from 10 min to 5, but otherwise am at a loss for ideas. Any and all suggestions will be greatly appreciated. Also, thanks so much for your answers to my recent questions. Sincerely, Carla Conway Western Fisheries Research Center 6505 NE 65th St Seattle, WA 98115 ph: 206-526-6282 fax: 206-526-6654 From godsgirlnow <@t> MSN.COM Wed Mar 23 17:17:54 2005 From: godsgirlnow <@t> MSN.COM (Roxanne Soto) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] copper stain for liver biopsies References: Message-ID: Jim, I do a few copper stains myself. I do the Lindquist Method (kit from Poly-Sci). This was a challenge for me to work up to do rapidly, and as you know rapid results are required if they are requesting a copper stain for a liver biopsy (Wilsons Disease). But, I finally worked out all the quirks and the Pathologists love it. But, make sure they know what they are looking for, I have had a few call me in and ask me to show them where the copper is in the control. By the way control sides also available from above. Any questions email me direct and I will help however I can. Roxanne ----- Original Message ----- From: Vickroy, Jim To: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 23, 2005 2:13 PM Subject: [Histonet] copper stain for liver biopsies We are looking for a procedure for staining for copper. In the past we have used an aldehyde fuchsin stain but our pathologist would like to use a rhodanine stain. Does anybody have a good procedure for staining copper and any suggestions where to get a kit or reagents? Jim Vickroy Memorial Medical Stain Springfield, Illinois _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgirlnow <@t> MSN.COM Wed Mar 23 17:20:43 2005 From: godsgirlnow <@t> MSN.COM (Roxanne Soto) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] copper stain for liver biopsies References: <4241F0C6.C59FE020@uwo.ca> Message-ID: I beg to differ with you John------ I use rhodanine as part of the lindquist method and it works beautifully. I think it is a matter of working it through until you get the results ---after going through several very expensive control slides I have it down to a perfect slide in a few hours instead of a few days. Roxanne ----- Original Message ----- From: John Kiernan To: Vickroy, Jim Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] copper stain for liver biopsies The reagent is p-dimethylaminobenzylidenerhodanine (pDMBR). Don't buy rhodanine; it's a different compound and it won't work. Also don't confuse with rhodamine and similarly named substances, which are completely unrelated. pDMBR 24 mg 100% ethanol 6 ml Before using, filter this solution into 45 ml of 0.065M sodium acetate (= 1.6% anhydrous, or 2% trihydrate) Put hydrated slides in the working solution either overnight at 37C or 11 min at 80C. Wash X6 in distilled water. Counterstain nuclei lightly with haemalum. Wash. Aqueous mountant. Cu-containing deposits brownish red. Sometimes it's necessary to release "masked" copper before doing the method. Before doing a histochemical method of this kind, read up about its mechanism, limitations and relations to other methods: Pearse's Histochemistry 4th ed Vol 2 (the 2nd or 3rd ed also OK). C. Chrukian's "Manual of the Special Stains Laboratory" 1997 or later edition. Irons et al 1977 Arch Path Lab Med 101,298-301 (This paper compares different methods for Cu.) -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ "Vickroy, Jim" wrote: > > We are looking for a procedure for staining for copper. In the past we > have used an aldehyde fuchsin stain but our pathologist would like to > use a rhodanine stain. Does anybody have a good procedure for staining > copper and any suggestions where to get a kit or reagents? > > Jim Vickroy > > Memorial Medical Stain > > Springfield, Illinois > _______________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Mar 23 17:56:51 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] problem with melanin staining reddish-brown by AEC chromogen In-Reply-To: Message-ID: What about bleaching the melanin? Carla M Conway Sent by: histonet-bounces@lists.utsouthwestern.edu 03/23/2005 02:48 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] problem with melanin staining reddish-brown by AEC chromogen Hello all, I am optimizing my protocol for a new antibody against the nucleocapsid protein of a fish rhabdovirus. Unfortunately, melanin pigment (especially large concentrations of it) is reddish-brown after staining with the AEC chromogen. This occurs on controls and test tissue, regardless of primary antibody dilution. Determining actual positive areas (if any) is problematic. I am using Dako's EnVision+ system and have been pleased with results using other antibodies. Switching to DAB then staining with Azure B is an option (this system uses either AEC or DAB), but I prefer to stay with AEC if at all possible. Comparing an H&E stained slide to its IHC counterpart is time consuming, but helpful. I have thought about reducing the chromogen incubation time from 10 min to 5, but otherwise am at a loss for ideas. Any and all suggestions will be greatly appreciated. Also, thanks so much for your answers to my recent questions. Sincerely, Carla Conway Western Fisheries Research Center 6505 NE 65th St Seattle, WA 98115 ph: 206-526-6282 fax: 206-526-6654 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katri <@t> cogeco.ca Wed Mar 23 19:15:38 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question References: <4241F0F3.188652CD@uwo.ca> Message-ID: <005c01c5300e$fdad36f0$6a9a9618@Katri> We write the numbers with the frosted end pointing north, since that's the way we file them standing up, the number easily to be read... Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "John Kiernan" To: "Histonet" Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] Re:Gloves ..... a question > "Marshall Terry Dr, Consultant Histopathologist" wrote: >> ... Slides are invariably labelled with the writing right >> way up, frosted end pointing North. This means the fingers >> and heel of the hand rest on the slide. That is the >> source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katri <@t> cogeco.ca Wed Mar 23 19:27:05 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] problem with melanin staining reddish-brown byAEC chromogen References: Message-ID: <006701c53010$994b0e10$6a9a9618@Katri> Carla, We actually choose to use AEC chromogen on human melanoma cases, rather than DAB. I don't know about fish, but human melanin is naturally brownish black, not red, so AEC staining is easy to pick out. Dab is not good. I have tried to do melanin bleach step with potassium permanganate before IHC, but it can diminish the antigenic reactivity and you may end up with false negative result. Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Jennifer MacDonald" To: "Carla M Conway" Cc: ; Sent: Wednesday, March 23, 2005 6:56 PM Subject: Re: [Histonet] problem with melanin staining reddish-brown byAEC chromogen > What about bleaching the melanin? > > > > > > Carla M Conway > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/23/2005 02:48 PM > > To > Histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] problem with melanin staining reddish-brown by AEC chromogen > > > > > > > Hello all, > > I am optimizing my protocol for a new antibody against the nucleocapsid > protein of a fish rhabdovirus. Unfortunately, melanin pigment (especially > large concentrations of it) is reddish-brown after staining with the AEC > chromogen. This occurs on controls and test tissue, regardless of primary > antibody dilution. Determining actual positive areas (if any) is > problematic. I am using Dako's EnVision+ system and have been pleased with > results using other antibodies. Switching to DAB then staining with Azure > B > is an option (this system uses either AEC or DAB), but I prefer to stay > with AEC if at all possible. Comparing an H&E stained slide to its IHC > counterpart is time consuming, but helpful. I have thought about reducing > the chromogen incubation time from 10 min to 5, but otherwise am at a loss > for ideas. > > Any and all suggestions will be greatly appreciated. > Also, thanks so much for your answers to my recent questions. > > Sincerely, > > Carla Conway > Western Fisheries Research Center > 6505 NE 65th St > Seattle, WA 98115 > ph: 206-526-6282 > fax: 206-526-6654 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dw18 <@t> uchicago.edu Wed Mar 23 20:48:38 2005 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re: BrdU, Histonet Digest, Vol 16, Issue 37 17 & 22 Message-ID: <16b60c2b.e1d870cb.81f5d00@m4500-00.uchicago.edu> Hi Kelly, Danielle and the Histonet RE: BrdU, Histonet Digest, Vol 16, Issue 37 17. BrdU protocol on frozen brain tissue (Kelly D Mcqueeney) 22. RE: BrdU protocol on frozen brain tissue (Danielle Crippen) I routinely do BrdU detection on 40um sections of PFA- perfused rat brain cut on a freezing sliding microtome. We all like to stick with an antibody that works, but I switched to Chemicon's MAB3518 a while ago. As it says in the product info, this doesn't require the harsh treatment with 2N HCl (and I also used to use Formamide/SSC at 65C to separate the strands of the histone-stripped DNA for use with Sigma- Aldrich's MAb). Advantages: a) it's faster b) you use your normal protocol and reagents **there's much less background and tissue shrinkage. (If you see how nasty - white & opaque - the sections look after 30' in 2N HCl at 37C, and which doesn't all go away with the borate neutralization, you'll understand why). c) it's economical. Chemicon recommends 1:5000 -10,000 for acid alcohol fixed cells. I use it at 10,000 (& 1:20,000 worked too!) overnight followed by Vector's biotinylated anti-mouse and ABC detection with Nickel & Cobalt intensification. I have no connection with Chemicon other than being a loyal customer. Good staining! -David A. Wright Section of Neurosurgery University of Chicago PS: I routinely spin my Ab tubes on arrival and immediately aliquot for freezing at -80C. Even with freshly calibrated pipettors, I find that there's always 5-10% less volume than the product info promises. This is with several suppliers - Chemicon, AbCam etc. Has anyone else noticed this annoying feature. Maybe they hope people will use straight from the tube and not notice??? -DAW ------------------------------ Message: 22 Date: Wed, 23 Mar 2005 07:46:23 -0800 From: "Danielle Crippen" Subject: RE: [Histonet] BrdU protocol on frozen brain tissue To: Dear Kelly, We've had great success with the following protocol using the monoclonal BrdU Ab from Roche (1:100) on mouse brain tissue (20um sections)and Vector's Avidin/Biotin blocking kit to block endogenous biotin: Immunocytochemical detection of BrdU-labled nuclei 1. Air dry samples for 20min. 2. cold MetOH fix for 20min @ -20C, air dry for 10min 3. Then incubated at 37C (slide moat) for 30min in 2N HCl .. 4. Sections are rinsed for 10 min at room temp in 0.1 M boric acid pH 8.5. 5. Incubate the sections for 10 min 1x PBS. 6. 15 min in 3% H2O2 in dH2O or MetOH 7. wash in PBS 5 min 8. Block in (1x PBS, 1% NHS, .1% BSA, .3% Triton) +Chicken anti MsIgG (2ug/ml) for 30 mins at RT 9. Sections are then incubated overnight at 4?C in primary antibody diluted in block buffer (PBS with 1% horse serum, 0.1% bovine serum albumin, and 0.3% Triton X-100) at its optimal concentration. 10. After being washed in PBS 3x5min, sections are incubated in biotinylated secondary antibody (Vector)diluted in block buffer for 2 hr at room temperature. 11. Make VECTASTAIN Elite ABC Reagent and allow VECTASTAIN Elite ABC Reagent to stand for about 30 minutes before use. 12. After three 5 min rinses in PBS. 13. Incubate sections for 30 minutes with VECTASTAIN ABC Reagent . 14. Wash slides for 5 minutes in PBS. 15. Incubate sections in peroxidase substrate solution (we use DAB kit from Vector) until desired stain intensity develops. 16. Rinse sections in tap water. 17. Dehydrate sections with 75%, 95%, 100% ethanol (3 min each) and Xylene (5 min). 18. Mount the sections with permanent mounting medium. Danielle Crippen Morphology Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org -----Original Message----- To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BrdU protocol on frozen brain tissue Does anyone have a protocol for BrdU IHC on frozen brain tissue? Thanks, Kelly McQueeney From JMacDonald <@t> mtsac.edu Wed Mar 23 21:31:19 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] H&E slides for HT practical exam In-Reply-To: <1111587579.42417afbcb4f2@imp.vet.upenn.edu> Message-ID: The fact that two people from one facility cut and stain their slides at the same time does not guarantee that they are of equal quality. The ASCP has criteria for grading the slides that all graders adhere too. There is a very fair system in place to ensure that all applicants receive fair grading. Jennifer MacDonald pmarcum@vet.upenn.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 03/23/2005 06:19 AM To lpwenk@sbcglobal.net cc histonet@lists.utsouthwestern.edu, Mark Adam Tarango Subject Re: [Histonet] H&E slides for HT practical exam I agree with Peggy. It is better to learn to be flexible and do excellent work than adhere to policy that only produces a ridgid stain regime. The goal is to produce slides that are of the highest quality for each tissue and stain. Unforutnately we have seen and heard from people taking the test for ASCP license in Histology who cut the same blocks and stianed the slides at the same time when one passed and one failed. I have yet to see anyone on the grading committee address this issue. Sorry to open this can of worms again however, when I took my test in the "70s I was told not to submit any animal tissue (I was doing research on animals only) or I would fail. I know that has changed and I did find a way (pre-HIPPA) to get my tissue and stain but it shows the differences in how we are treated and graded over the years. Other than what you stated for H&E is there a true standard for each stain or does it depend on the person grading and what they like or see. Pam Marcum Quoting lpwenk@sbcglobal.net: > Slides can be stained all together or one at a time. There is no way anyone > can tell. > > In fact, time in each solution can be different for each tissue stained. > > What is important is that EACH slide be well stained - good crisp nuclei > with chromatin pattern, a minimum of three shades of eosin that does not > overwhelm the hematoxylin, etc.. > > If that takes taking each slide through the different solutions/stains at > different time intervals, repeating and tweaking the stain 20 times with > different time intervals from what you normally do, and changing to > different times for each block depending upon the tissue/length of time it > has been fixed/tissue thickness/etc. - then do it. It is more much more > important to know what to do to produce a well stained slide, than to > rigidly adhere to the same steps/times. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > ----- Original Message ----- > From: "Mark Adam Tarango" > To: > Sent: Tuesday, March 22, 2005 3:52 PM > Subject: [Histonet] H&E slides for HT practical exam > > > > Hi everyone. I have a co-worker who insists that she must stain all her > H&E and slides for her practical exam together at once. My supervisor and I > haven't ever heard this, and I'm hoping someone can give a reply as to > whether or not this is something that is required. She is conviced that if > she doesn't stain them all together (her H&E slides), someone will know and > fail her again. If Peggy Wenk or Jennifer MacDonald are watching, we'd > like to her from you especially. > > > > > > Thanks, > > > > > > Mark Tarango > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Megan.Clarke <@t> hnehealth.nsw.gov.au Thu Mar 24 01:33:34 2005 From: Megan.Clarke <@t> hnehealth.nsw.gov.au (Megan Clarke) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] fixatives Message-ID: Hi to all We have had a problem with accessing the histonet website...but we're on track again. Whoop dee doo! I require some information regarding these fixatives. If anyone out in Histonetland is is using any of these fixatives or has a reference for me , I would be very happy and thankful. CBA formalin JB fixative Excell Thank you for your help in advance with my query. HAPPY EASTER and HAPPY HOLIDAYS Zenobia Haffajee Hunter Area New England Health IHC dept Newcastle Australia From ree3 <@t> leicester.ac.uk Thu Mar 24 04:08:53 2005 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] apoptosis mice Message-ID: Which is the best marker for apoptosis in the mouse using paraffin sections, have used cleaved caspase 3 some time ago and wonder what else is available these days. Thanks Richard Edwards MRC TOXICOLOGY UNIT...U.K..... From Terry.Marshall <@t> rothgen.nhs.uk Thu Mar 24 06:21:57 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question Message-ID: Yes - I'm sure that is the reason. Otherwise, it would make more sense to do it upside down. Of course since there are usually 2 labelling events, pencil then paper label, there is no reason why the first shouldn't be upside down and the second, done on the stained and coverslipped slide, the right way up (for storage). How do you cope with reading labels if your slides are stored with upside down writing John? Or is upside-down reading just another skill you have? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Katri Tuomala [mailto:katri@cogeco.ca] Sent: 24 March 2005 01:16 To: John Kiernan; Histonet Subject: Re: [Histonet] Re:Gloves ..... a question We write the numbers with the frosted end pointing north, since that's the way we file them standing up, the number easily to be read... Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "John Kiernan" To: "Histonet" Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] Re:Gloves ..... a question > "Marshall Terry Dr, Consultant Histopathologist" wrote: >> ... Slides are invariably labelled with the writing right >> way up, frosted end pointing North. This means the fingers >> and heel of the hand rest on the slide. That is the >> source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Thu Mar 24 06:30:12 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question Message-ID: Not all slides are filed with a paper label. In our lab we routinely cut multiple unstained sections for future testing. We usually use an automatic slide etcher/labeler, but when they are not working properly we write manually. I lay a Kaydry towel across the lower part of my slides so my hand does not directly rest on the slide when I must label a large quantity of slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 24, 2005 7:22 AM To: Katri Tuomala; John Kiernan; Histonet Subject: RE: [Histonet] Re:Gloves ..... a question Yes - I'm sure that is the reason. Otherwise, it would make more sense to do it upside down. Of course since there are usually 2 labelling events, pencil then paper label, there is no reason why the first shouldn't be upside down and the second, done on the stained and coverslipped slide, the right way up (for storage). How do you cope with reading labels if your slides are stored with upside down writing John? Or is upside-down reading just another skill you have? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Katri Tuomala [mailto:katri@cogeco.ca] Sent: 24 March 2005 01:16 To: John Kiernan; Histonet Subject: Re: [Histonet] Re:Gloves ..... a question We write the numbers with the frosted end pointing north, since that's the way we file them standing up, the number easily to be read... Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "John Kiernan" To: "Histonet" Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] Re:Gloves ..... a question > "Marshall Terry Dr, Consultant Histopathologist" wrote: >> ... Slides are invariably labelled with the writing right way up, >> frosted end pointing North. This means the fingers and heel of the >> hand rest on the slide. That is the source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Mar 24 07:08:37 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] apoptosis mice Message-ID: I'm using cleaved caspase 3 routinely for murine FFPE sections. Biocare has a good antibody. Jacqueline M. O'Connor HT(ASCP) Assistant Scientist GPRD Cancer Research Abbott Laboratories, Abbott Park, IL Jackie.OConnor@abbott.com "Edwards, R.E." Sent by: histonet-bounces@lists.utsouthwestern.edu 03/24/2005 04:08 AM To: cc: Subject: [Histonet] apoptosis mice Which is the best marker for apoptosis in the mouse using paraffin sections, have used cleaved caspase 3 some time ago and wonder what else is available these days. Thanks Richard Edwards MRC TOXICOLOGY UNIT...U.K..... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Thu Mar 24 07:50:24 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0F0@bhrv-nt-11.bhrv.nwest.nhs.uk> But surely you should write on the frosted end pointing south, so you don't contaminate the slide and then label on the other end? That way you can be sure that the writing 'down south' matches the label 'up north' (or is it the other way round)? If you stuck the label over the writing then how can the Pathologist be sure it is the correct slide? Using a mirror or X-Ray vision? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Marshall Terry Dr,Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 24 March 2005 12:22 To: Katri Tuomala; John Kiernan; Histonet Subject: RE: [Histonet] Re:Gloves ..... a question[Scanned] Yes - I'm sure that is the reason. Otherwise, it would make more sense to do it upside down. Of course since there are usually 2 labelling events, pencil then paper label, there is no reason why the first shouldn't be upside down and the second, done on the stained and coverslipped slide, the right way up (for storage). How do you cope with reading labels if your slides are stored with upside down writing John? Or is upside-down reading just another skill you have? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Katri Tuomala [mailto:katri@cogeco.ca] Sent: 24 March 2005 01:16 To: John Kiernan; Histonet Subject: Re: [Histonet] Re:Gloves ..... a question We write the numbers with the frosted end pointing north, since that's the way we file them standing up, the number easily to be read... Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "John Kiernan" To: "Histonet" Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] Re:Gloves ..... a question > "Marshall Terry Dr, Consultant Histopathologist" wrote: >> ... Slides are invariably labelled with the writing right >> way up, frosted end pointing North. This means the fingers >> and heel of the hand rest on the slide. That is the >> source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Thu Mar 24 08:22:03 2005 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] H&E slides for HT practical exam In-Reply-To: References: Message-ID: <1111674123.4242cd0bc4955@imp.vet.upenn.edu> Jennifer, I understand the comment about two people sectioning differently etc and staining can look different even when sections are all stained together on a stainer. However, when the pathologists who reviews (for students) the same sets of slides and see no difference it is not as clear cut as you see it. I have been seeing and hearing the same story for years and ASCP does not clarify beyond a description what they want. What I have never understood is why the guidelines for histology registry have not been clearly defined and with the number of people grading given a standard of stain/slides to work from. I am sorry that in my opinion. ASCP takes our money and wants us to register and doesn't take as much time for a critical to care area as they do for other areas of the clinical and research laboratories. I have not ASCP to either responsive or caring about our field over the almost 30 years I have been registered. They only get my money yearly because they are the only current body with a national register and I have always done CEU so that is not an issue for me. Pam Marcum Quoting Jennifer MacDonald : > The fact that two people from one facility cut and stain their slides at > the same time does not guarantee that they are of equal quality. The ASCP > has criteria for grading the slides that all graders adhere too. There is > a very fair system in place to ensure that all applicants receive fair > grading. > > Jennifer MacDonald > > > > > pmarcum@vet.upenn.edu > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/23/2005 06:19 AM > > To > lpwenk@sbcglobal.net > cc > histonet@lists.utsouthwestern.edu, Mark Adam Tarango > > Subject > Re: [Histonet] H&E slides for HT practical exam > > > > > > > I agree with Peggy. It is better to learn to be flexible and > do excellent work than adhere to policy that only produces a ridgid stain > regime. > The goal is to produce slides that are of the highest quality for each > tissue > and stain. > > Unforutnately we have seen and heard from people taking the test for ASCP > license > in Histology who cut the same blocks and stianed the slides at the same > time > when one passed > and one failed. I have yet to see anyone on the grading committee address > this > issue. > > Sorry to open this can of worms again however, when I took my test in the > "70s I > was told > not to submit any animal tissue (I was doing research on animals only) or > I > would fail. I know > that has changed and I did find a way (pre-HIPPA) to get my tissue and > stain but > it shows the differences > in how we are treated and graded over the years. Other than what you > stated > for H&E is there a true > standard for each stain or does it depend on the person grading and what > they > like or see. > > Pam Marcum > > Quoting lpwenk@sbcglobal.net: > > > Slides can be stained all together or one at a time. There is no way > anyone > > can tell. > > > > In fact, time in each solution can be different for each tissue stained. > > > > What is important is that EACH slide be well stained - good crisp nuclei > > with chromatin pattern, a minimum of three shades of eosin that does not > > overwhelm the hematoxylin, etc.. > > > > If that takes taking each slide through the different solutions/stains > at > > different time intervals, repeating and tweaking the stain 20 times > with > > different time intervals from what you normally do, and changing to > > different times for each block depending upon the tissue/length of time > it > > has been fixed/tissue thickness/etc. - then do it. It is more much more > > important to know what to do to produce a well stained slide, than to > > rigidly adhere to the same steps/times. > > > > Peggy A. Wenk, HTL(ASCP)SLS > > William Beaumont Hospital > > Royal Oak, MI 48073 > > > > ----- Original Message ----- > > From: "Mark Adam Tarango" > > To: > > Sent: Tuesday, March 22, 2005 3:52 PM > > Subject: [Histonet] H&E slides for HT practical exam > > > > > > > Hi everyone. I have a co-worker who insists that she must stain all > her > > H&E and slides for her practical exam together at once. My supervisor > and I > > haven't ever heard this, and I'm hoping someone can give a reply as to > > whether or not this is something that is required. She is conviced that > if > > she doesn't stain them all together (her H&E slides), someone will know > and > > fail her again. If Peggy Wenk or Jennifer MacDonald are watching, > we'd > > like to her from you especially. > > > > > > > > > Thanks, > > > > > > > > > Mark Tarango > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Terry.Marshall <@t> rothgen.nhs.uk Thu Mar 24 08:23:53 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question[Scanned] Message-ID: We trust you implicitly of course. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 24 March 2005 13:50 To: Marshall Terry Dr, Consultant Histopathologist; Katri Tuomala; John Kiernan; Histonet Subject: RE: [Histonet] Re:Gloves ..... a question[Scanned] But surely you should write on the frosted end pointing south, so you don't contaminate the slide and then label on the other end? That way you can be sure that the writing 'down south' matches the label 'up north' (or is it the other way round)? If you stuck the label over the writing then how can the Pathologist be sure it is the correct slide? Using a mirror or X-Ray vision? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Marshall Terry Dr,Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 24 March 2005 12:22 To: Katri Tuomala; John Kiernan; Histonet Subject: RE: [Histonet] Re:Gloves ..... a question[Scanned] Yes - I'm sure that is the reason. Otherwise, it would make more sense to do it upside down. Of course since there are usually 2 labelling events, pencil then paper label, there is no reason why the first shouldn't be upside down and the second, done on the stained and coverslipped slide, the right way up (for storage). How do you cope with reading labels if your slides are stored with upside down writing John? Or is upside-down reading just another skill you have? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Katri Tuomala [mailto:katri@cogeco.ca] Sent: 24 March 2005 01:16 To: John Kiernan; Histonet Subject: Re: [Histonet] Re:Gloves ..... a question We write the numbers with the frosted end pointing north, since that's the way we file them standing up, the number easily to be read... Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "John Kiernan" To: "Histonet" Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] Re:Gloves ..... a question > "Marshall Terry Dr, Consultant Histopathologist" wrote: >> ... Slides are invariably labelled with the writing right >> way up, frosted end pointing North. This means the fingers >> and heel of the hand rest on the slide. That is the >> source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fijikaiviti <@t> hotmail.com Thu Mar 24 08:37:45 2005 From: fijikaiviti <@t> hotmail.com (Anurag Sharma) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Natural Dyes: Beet Juice Message-ID: Hi, I am a first year Med Lab Science student and am doing a project on "Natural Dyes". Has anyone here ever tried using Beet juice to stain tissues? If yes, could you please tell me something about it (like the extraction procedure, mordant used, pH and what does it stain). Is there any other Natural dye you would suggest me to use? Dev From pwg1 <@t> cdc.gov Thu Mar 24 08:37:48 2005 From: pwg1 <@t> cdc.gov (Greer, Patricia) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] fixatives Message-ID: Megan, Here is the "recipe" for the JB fixative which we have used for fixation of mouse tissues in which we wanted to detect CD3, CD4 and CD8. JB Fixative 0.1 M Tris Buffer, pH 7.4 1000ml Calcium acetate 0.5 g Zinc Acetate 5.0 g Zinc Chloride 5.0 g Mix to dissolve. The final pH will be approximately 6.8 (6.5-7.0). Do not readjust the pH, as this will cause the zinc to come out of solution. Small amounts of insoluable zinc oxychloride often contaminates zinc chloride. This may result in a very small amount of white precipitate. This will not cause any problems but can be removed by filtering the solution. Fix tissue for 24-48 hours, then transfer tissues to 70% ethanol. Reference: Beckstead JH. A Simple Technique for Preservation of Fixative-sensitive Antigens in Paraffin-embedded Tissues: Addendum (Letter to the Editor). J Histochem Cytochem 1994; 43:345 Pat Greer Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS G-32 Atlanta, GA 30333 I require some information regarding these fixatives. If anyone out in Histonetland is is using any of these fixatives or has a reference for me , I would be very happy and thankful. CBA formalin JB fixative Excell Thank you for your help in advance with my query. HAPPY EASTER and HAPPY HOLIDAYS Zenobia Haffajee Hunter Area New England Health IHC dept Newcastle Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sluhisto <@t> yahoo.com Thu Mar 24 08:51:37 2005 From: sluhisto <@t> yahoo.com (Histology SLU) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Alcian Green Dye Message-ID: <20050324145137.83325.qmail@web51005.mail.yahoo.com> Hello All: Just got off of the phone with sigma and they no longer carry alcian green and do not have another source to suggest. Can anyone out there share their source that is not sigma? Thanks, in advance. Have a blessed Easter. Susan __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From vazquezr <@t> ohsu.edu Thu Mar 24 09:04:54 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question Message-ID: Yep!! That's the way we do it and any other place I have ever worked... Robyn OHSU >>> "Katri Tuomala" 03/23/05 5:15 PM >>> We write the numbers with the frosted end pointing north, since that's the way we file them standing up, the number easily to be read... Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "John Kiernan" To: "Histonet" Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] Re:Gloves ..... a question > "Marshall Terry Dr, Consultant Histopathologist" wrote: >> ... Slides are invariably labelled with the writing right >> way up, frosted end pointing North. This means the fingers >> and heel of the hand rest on the slide. That is the >> source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Thu Mar 24 09:11:11 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question[Scanned] Message-ID: Kemlo, The pathologist needs to be confident in their histotech to make sure that it is the correct patient, but still have a mirror handy just in case one slips by the histo crew. Generally there is more than one tech and you should all watch out for each other and make sure everything is in order before it even gets to the doc. Just my humble opinion Robyn OHSU >>> Kemlo Rogerson 03/24/05 5:50 AM >>> But surely you should write on the frosted end pointing south, so you don't contaminate the slide and then label on the other end? That way you can be sure that the writing 'down south' matches the label 'up north' (or is it the other way round)? If you stuck the label over the writing then how can the Pathologist be sure it is the correct slide? Using a mirror or X-Ray vision? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Marshall Terry Dr,Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 24 March 2005 12:22 To: Katri Tuomala; John Kiernan; Histonet Subject: RE: [Histonet] Re:Gloves ..... a question[Scanned] Yes - I'm sure that is the reason. Otherwise, it would make more sense to do it upside down. Of course since there are usually 2 labelling events, pencil then paper label, there is no reason why the first shouldn't be upside down and the second, done on the stained and coverslipped slide, the right way up (for storage). How do you cope with reading labels if your slides are stored with upside down writing John? Or is upside-down reading just another skill you have? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Katri Tuomala [mailto:katri@cogeco.ca] Sent: 24 March 2005 01:16 To: John Kiernan; Histonet Subject: Re: [Histonet] Re:Gloves ..... a question We write the numbers with the frosted end pointing north, since that's the way we file them standing up, the number easily to be read... Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "John Kiernan" To: "Histonet" Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] Re:Gloves ..... a question > "Marshall Terry Dr, Consultant Histopathologist" wrote: >> ... Slides are invariably labelled with the writing right >> way up, frosted end pointing North. This means the fingers >> and heel of the hand rest on the slide. That is the >> source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Mar 24 12:15:16 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Natural Dyes: Beet Juice In-Reply-To: References: Message-ID: <424303B4.9070609@umdnj.edu> Hi Anurag: Get yourself a copy of "Natural dyes and home dyeing" by Rita Adrosko. It is published by Dover and is not expensive. Lots of recipes and a pretty good bibliography. Geoff Anurag Sharma wrote: >Hi, > >I am a first year Med Lab Science student and am doing a project on "Natural Dyes". Has anyone here ever tried using Beet juice to stain tissues? If yes, could you please tell me something about it (like the extraction procedure, mordant used, pH and what does it stain). Is there any other Natural dye you would suggest me to use? > >Dev >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From NMargaryan <@t> childrensmemorial.org Thu Mar 24 09:38:38 2005 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] FAK-576 antibody on FFPE Message-ID: <63B8B599DE283148B92E83C78B32C15D8B4AE1@cmhexbe2.childrensmemorial.org> Would anyone care to share their experiences and protocol with FAK-576 antibody on FFPE tissue? I have protocol for FAK 397, it working well. I will apretiate for any response :-) Naira Naira V. Margaryan, Ph.D, D.V.M. Research Associate II Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-880-4000/5-6740 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. From mcauliff <@t> umdnj.edu Thu Mar 24 12:53:05 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] which new cryostat? In-Reply-To: References: Message-ID: <42430C91.9070402@umdnj.edu> So yesterday one of my colleagues calls me up and says "Which new cryostat should I buy?". My reply was "I'll ask Histonet!." So what is the collective wisdom? The new cryostat will be used for mouse and rat brain and spinal cord for the most part. It should be easy to use and maintain since it will be used by facutly, post-docs, graduate students, technicians and medical students doing summer research projects. Disposable knives will be used in most cases. Replys by vendors will be welcomed. Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Janet.Bonner <@t> FLHOSP.ORG Thu Mar 24 09:59:59 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question[Scanned] Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41EF@fh2k093.fhmis.net> Actually, we look at the writing THROUGH the slide from the back when there is a label on it. This is interesting. All this time we thought the waterbath was to blame! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: 'Marshall Terry Dr,Consultant Histopathologist'; Katri Tuomala; John Kiernan; Histonet Sent: 3/24/2005 8:50 AM Subject: RE: [Histonet] Re:Gloves ..... a question[Scanned] But surely you should write on the frosted end pointing south, so you don't contaminate the slide and then label on the other end? That way you can be sure that the writing 'down south' matches the label 'up north' (or is it the other way round)? If you stuck the label over the writing then how can the Pathologist be sure it is the correct slide? Using a mirror or X-Ray vision? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Marshall Terry Dr,Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 24 March 2005 12:22 To: Katri Tuomala; John Kiernan; Histonet Subject: RE: [Histonet] Re:Gloves ..... a question[Scanned] Yes - I'm sure that is the reason. Otherwise, it would make more sense to do it upside down. Of course since there are usually 2 labelling events, pencil then paper label, there is no reason why the first shouldn't be upside down and the second, done on the stained and coverslipped slide, the right way up (for storage). How do you cope with reading labels if your slides are stored with upside down writing John? Or is upside-down reading just another skill you have? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Katri Tuomala [mailto:katri@cogeco.ca] Sent: 24 March 2005 01:16 To: John Kiernan; Histonet Subject: Re: [Histonet] Re:Gloves ..... a question We write the numbers with the frosted end pointing north, since that's the way we file them standing up, the number easily to be read... Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "John Kiernan" To: "Histonet" Sent: Wednesday, March 23, 2005 5:42 PM Subject: Re: [Histonet] Re:Gloves ..... a question > "Marshall Terry Dr, Consultant Histopathologist" wrote: >> ... Slides are invariably labelled with the writing right >> way up, frosted end pointing North. This means the fingers >> and heel of the hand rest on the slide. That is the >> source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelly.mcqueeney <@t> bms.com Thu Mar 24 10:02:45 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] which new cryostat? In-Reply-To: <42430C91.9070402@umdnj.edu> References: <42430C91.9070402@umdnj.edu> Message-ID: <4242E4A5.4030109@bms.com> I loved the high-end Vibratome cryostat. It has all of the same bells and whistles as Richard Allan, but was $15,000 cheaper. The roll plate is more secure and there is a lot of room in the cryostat, you can even store a slide box over the microtome. Good luck, Kelly Geoff McAuliffe wrote: > So yesterday one of my colleagues calls me up and says "Which new > cryostat should I buy?". My reply was "I'll ask Histonet!." So what is > the collective wisdom? The new cryostat will be used for mouse and rat > brain and spinal cord for the most part. It should be easy to use and > maintain since it will be used by facutly, post-docs, graduate > students, technicians and medical students doing summer research > projects. Disposable knives will be used in most cases. Replys by > vendors will be welcomed. > > Geoff > -- > > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu > ********************************************** > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Matthew_Frank <@t> URMC.Rochester.edu Thu Mar 24 10:11:19 2005 From: Matthew_Frank <@t> URMC.Rochester.edu (Frank, Matthew) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Pb staining Message-ID: Chuck Churukian has asked me to post the following for him. Does anyone out there have experience with Lead staining? Currently he is working with the sodium rhodizonate method. Are there other methods that anyone would recommend? Also, control blocks seem to be hard to come by, any sources or suggestions. Thank you all in advance for your input. From jessica_butler <@t> oz.ped.emory.edu Thu Mar 24 10:17:34 2005 From: jessica_butler <@t> oz.ped.emory.edu (Jessica Butler) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] (no subject) Message-ID: Hi- I need some information on staining cytospun cells. The cells in question are alveolar macrophages, and I am using the Prussian Blue method for staining iron. First, should the cells be treated with Trition X, and after staining do the cells need to be dehydrated to coverslip? Finally, what type of coverslipping medium should be used? We are currently using Permount with poor results. Thank you for any help in advance, Jessica From n.cragg <@t> epistem.co.uk Thu Mar 24 10:14:24 2005 From: n.cragg <@t> epistem.co.uk (ncragg) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Stability of Phosphorylated Antigens in Paraffin Sections Message-ID: <01C5308C.8C560710.n.cragg@epistem.co.uk> Hello, Does anyone have information, knowledge, experience or publications on the stability of phosphorylated antigens / epitopes in FFPE sections? Is it important to carry out any phospho-specific IHC asap after sectioning? Does the phosphorylated sites decrease in antigenicity while being stored in sections or should they be preserved if sections are stored at 4degC as is the case with other (unphosphorylated) antigens? Not sure whether this has been discussed before, have searched the archives, but maybe not using the correct search terms. Any comments or thoughts would be greatly appreciated. Nicola Cragg Manchester, UK From Terry.Marshall <@t> rothgen.nhs.uk Thu Mar 24 10:28:06 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Dark ground illumination for silver Message-ID: I don't know why, but all my best stories are against myself. This is the latest. I recently had a skin in which there was finely particulate pigment, rather deep, and slightly but definitely favouring sweat gland basement membranes. "Ah" thought I - "got it - silver". The next port of call was microbiology for a darkground microscope. Delighted I was when the material shone through like the stars in the desert. As readers may anticipate, that is exactly what silver was supposed to do. I sent it to the local centre of excellence for EDAX, having already spoken to the recipient and telling him that he would be receiving it, but I was going to do an iron and melanin stain first. It got neglected, and I suddenly refound it and in a hurry sent it off without doing these stain. Yes readers, the report came back that they had stained it for iron and it was positive. I have implored the recipient to not tell anyone in exchange for good money, but I'm sure it will be too late. So, what is the specificity/range of things that shine through darkground illumination? I can't find the answer. From the fact that this test was mentioned for silver, there seemed to me to be an implication that other common things like iron would not so behave. Can anyone put me right? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From padewo <@t> optonline.net Thu Mar 24 10:52:43 2005 From: padewo <@t> optonline.net (padewo@optonline.net) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Ventana Symphony and Cost analysis Message-ID: <114da44114fb4c.114fb4c114da44@optonline.net> I recently noted somebody mentioned a new Ventana product for H&E staining called the Symphony, I'm having some trouble getting information on it from the web. Would love to hear any info about it. I am also looking for any cost analysis programs to determine what volume we need to be at to make automating our H&E staining a profitable decision. Frank Rindez From KJohnson <@t> med.miami.edu Thu Mar 24 11:15:57 2005 From: KJohnson <@t> med.miami.edu (Johnson, Kevin) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question Message-ID: <3CE8FC83E1E9A44EB0652CAD2F328C096B428E@MEDEX08.ad.med.miami.edu> I label my slides by first placing them in a cardboard 20-slide folder with the cover removed, then placing an open folder over the slides, exposing only the frosted portion. The writing hand rests on the cardboard rather than on the slide, and a single finger on the edge of the frosted portion stabilizes the slide for labeling. Kevin Johnson University of Miami Diabetes Research Institute Miami, FL > -----Original Message----- > From: John Kiernan [mailto:jkiernan@uwo.ca] > Sent: Wednesday, March 23, 2005 5:43 PM > To: Histonet > Subject: Re: [Histonet] Re:Gloves ..... a question > > "Marshall Terry Dr, Consultant Histopathologist" wrote: > > ... Slides are invariably labelled with the writing right > > way up, frosted end pointing North. This means the fingers > > and heel of the hand rest on the slide. That is the > > source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > From Barry.R.Rittman <@t> uth.tmc.edu Thu Mar 24 11:29:47 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Dark ground illumination for silver Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0015BFC43@UTHEVS3.mail.uthouston.edu> Terry There are a lot of material that will show up under darkfield microscopy, either due to their normal refractile nature or to the staining process. Silver granules will show beautifully but darkfield is not specific for silver or for spirochaetes which also show up well. The only requirement is that materials are able to refract the light sufficiently so that it will enter the most peripheral portions of the darkfield condenser, preferably a cardioid condenser. I often use darkfield for looking at H and E slides. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 24, 2005 10:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Dark ground illumination for silver I don't know why, but all my best stories are against myself. This is the latest. I recently had a skin in which there was finely particulate pigment, rather deep, and slightly but definitely favouring sweat gland basement membranes. "Ah" thought I - "got it - silver". The next port of call was microbiology for a darkground microscope. Delighted I was when the material shone through like the stars in the desert. As readers may anticipate, that is exactly what silver was supposed to do. I sent it to the local centre of excellence for EDAX, having already spoken to the recipient and telling him that he would be receiving it, but I was going to do an iron and melanin stain first. It got neglected, and I suddenly refound it and in a hurry sent it off without doing these stain. Yes readers, the report came back that they had stained it for iron and it was positive. I have implored the recipient to not tell anyone in exchange for good money, but I'm sure it will be too late. So, what is the specificity/range of things that shine through darkground illumination? I can't find the answer. From the fact that this test was mentioned for silver, there seemed to me to be an implication that other common things like iron would not so behave. Can anyone put me right? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peoshel <@t> wisc.edu Thu Mar 24 11:53:30 2005 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Dark ground illumination for silver In-Reply-To: References: Message-ID: Hum. I wonder if this is a case of Wilde's "two people separated by a common language"? By "darkground" microscope, do you mean a darkfield microscope? If not, then ignore what I'm writing, if yes ... "So, what is the specificity/range of things that shine through darkground illumination?" There are 2 basic answers. For opaque objects like many mineral deposits, anything that diffracts light well enough will be bright against a black background. For objects that transmit light, then anything that has a refractive index sufficiently different from the surrounding material will be bright against a black background. "Sufficiently different" just means being different enough to scatter the light, so ordinary biological samples are good for darkfield microscopy. The same principle works for darkfield transmission electron microscopy. The basic principle is a hollow cone of light, of a diameter exceeding the NA of the objective, is projected through the sample. Undeflected -- by diffraction or refraction -- light is not collected, and so the field is black. Diffracted or refracted light is directed into the objective and so imaged. This also allows detection (although not resolution) of sub-resolution structures, such as bacterial flagellae. Darkfield microscopy was developed mostly for biological samples, I believe. It's great for cells, bacteria, protozoans, tiny critters in pond water ... But useless for identifying minerals or metals beyond "Yep, somthing's there." Here's my chance to plug one of my favorite books I don't see mentioned on Histonet: E.M. Slayter and H.S. Slayter, "Light and Electron Microscopy". No how-to, but excellent discussions of how and why microscopes do what they do. If you meant some other kind of microscope, like one used to search for metal ores in black loam soil, then all that doesn't apply. Phil >I don't know why, but all my best stories are against myself. This >is the latest. > >I recently had a skin in which there was finely particulate pigment, >rather deep, and slightly but definitely favouring sweat gland >basement membranes. "Ah" thought I - "got it - silver". >The next port of call was microbiology for a darkground microscope. >Delighted I was when the material shone through like the stars in >the desert. As readers may anticipate, that is exactly what silver >was supposed to do. > >I sent it to the local centre of excellence for EDAX, having already >spoken to the recipient and telling him that he would be receiving >it, but I was going to do an iron and melanin stain first. >It got neglected, and I suddenly refound it and in a hurry sent it >off without doing these stain. >Yes readers, the report came back that they had stained it for iron >and it was positive. >I have implored the recipient to not tell anyone in exchange for >good money, but I'm sure it will be too late. > >So, what is the specificity/range of things that shine through >darkground illumination? I can't find the answer. From the fact that >this test was mentioned for silver, there seemed to me to be an >implication that other common things like iron would not so behave. > >Can anyone put me right? > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From gcallis <@t> montana.edu Thu Mar 24 12:01:49 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] upside down labels and glove issues In-Reply-To: References: Message-ID: <6.0.0.22.1.20050324082524.01b08f40@gemini.msu.montana.edu> Hmmm never had a problem reading upside down, backwards labels! My paraffin blocks, trimmed and cooling on ice block have numbers upside down. I also stack or line up slides with labels upside down (northern labels facing south??) for my style of section pickup. Hoever, slides are top labeled for easy reading/retrieving from slide drawer files. Cut blocks end up with labels upside down, backwards all the time. My other tech does most impeccable pencil ("eastern" or sideways for slide box filing) labeling AFTER she picks up her sections (literally hundreds of sections!) AND is required to wear gloves 100% of time for ALL phases of paraffin rodent prion reserach project from processing through coverslipping. Her sections/slides are spotless - no graphite blobs - an equivalent to squamous cells. She is the neatest, most disciplined microtomist I have had the pleasure to mentor. I back her up occasionally, and gloves never interfere with any dexterity we need for prion project paraffin work. All my other paraffin projects from embedding to sectioning is done gloveless although an occasional escapee sq cell or two from a mystery source shows up. Fingers never go swimming (seen some immerse half their fist into waterbath!) and "touchy feely" all over a glass slide is forbidden - students learn latter rule very quickly from this Old Mother HenTech!! We wear gloves that fit our hands smoothly, unwrinkled over fingertips, and not too tight to cut circulation. We did take time to find flexible nitrile gloves rather than experience "floppy glove syndrome" on fingertips. Optional, washable silk liners (for R and L hands www.wintersilks.com) soak up in-glove perspiration with bonus of keeping hands warm for hours of cryosectioning. Interesting how we perform our technics, gloveless or not, but it has been entertaining - plus learned some things Thanks folks Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From benardsolomona <@t> yahoo.com Thu Mar 24 12:02:17 2005 From: benardsolomona <@t> yahoo.com (Solomon Adebayo) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re: 'Natural Dyes) Message-ID: <20050324180217.5973.qmail@web50904.mail.yahoo.com> You can try the (aq) extract of hibiscus sabdariffa i.e red leaves of sorel plant at 56oC. Benard Solomon, Pathology Dept., University of Ilorin Teaching Hospital, Kwara State, Nigeria. benardsolomona@yahoo.com __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From jkiernan <@t> uwo.ca Thu Mar 24 12:21:46 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Upside down labels (was Re:Gloves ... a question) References: Message-ID: <4243053A.303D3EE4@uwo.ca> "Marshall Terry Dr, Consultant Histopathologist" wrote: > ... How do you cope with reading labels if your slides are > stored with upside down writing John? Or is upside-down > reading just another skill you have? --------------------- I keep stained slides horizontally - in slotted boxes that hold 100, with lines for notes on the inside of the lid. For long-term archiving I store slides quite tightly packed, in the cardboard boxes that new slides come in. Either way, it's necessary to take a slide out to read the label, so it doesn't matter which way up it is. Slides that I'm working with are usually in flat trays, so again it's easy to read the labels. I was taught to use plain slides and label with a diamond pencil (very permanent) before staining, and did this for several years. It's hard work when doing a big batch, and technicians didn't like it. Occasionally bits of ground glass would get on the sections. Pencil on a frosted end is less effort, doesn't often dump graphite on the sections, and resists anything but rubbing. A smear of diluted mounting medium makes the label permanent. I've never used paper labels. Can't see the point. Our slides for teaching histology and neuroanatomy have paper labels; they get dirty and tatty with the passage of years. No, I don't do upside-down reading, but 10 years ago almost to the day I wrote a little program called UPSI.EXE for inverting small ASCII files. It was useful when making diagrams etc with the line, box & block characters, but that's not a thing people do any more. The program can also upsify (reverse the order of) the lines of a list. I don't know another way to do that job, except by multiple cutting and pasting. John Kiernan London, Canada. ---------------------------------------------------------- From SHargrove <@t> urhcs.org Thu Mar 24 12:25:23 2005 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Numbering slides Message-ID: I am always amazed when I take the time to read this. There are so many great ideas out there. I do not understand why intelligent people still forward all the mail with a reply. I just want to add .5 cents to the "writing on the slides" issue". We put the number on the edge of the west side. If the north is the top . On a clean counter top, wearing gloves, take them out just as they are packaged in the box, push them back , exposing the edges and write away, a box of 75 or so is numbered quickly. Then push the last one back towards the front and return to box. Works for all of us. The additional letters and numbers go below this. They are easy to read without a mirror. S Hargrove URHCS Wichita Falls, Texas The documents inside this electronic transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled, unless otherwise required by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you received this electronic transmission in error, please notify the sender immediately to arrange for return. From kmcarr0 <@t> uky.edu Thu Mar 24 12:20:21 2005 From: kmcarr0 <@t> uky.edu (Kim Carrico) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] p0 antibody Message-ID: <200503241815.j2OIFTx03246@mr1.uky.edu> Does anyone know of a vendor for a p0 antibody? I am trying to stain rat spinal cord sections and have been looking for over a month for a commercially available p0 antibody, but haven't been able to find any. The papers I've found where it has been used usually state that it was a gift from someone. I only need enough to stain 2 slides, but I can't find anyone that has it. Any suggestions? Kim Carrico University of Kentucky SCoBIRC MS 274 859-323-5359 From jkiernan <@t> uwo.ca Thu Mar 24 12:38:45 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Natural Dyes: Beet Juice References: Message-ID: <42430935.E4804FB8@uwo.ca> Chapter 17 in the 9th (1977) edition of Conn's Biological Stains, by RD Lillie, has several references to use of flower and fruit extracts, alone and with aluminium salts, usually producing nuclear stains. He doesn't mention beetroot juice. John Kiernan London, Canada. ------------------------------------------------ Anurag Sharma wrote: > > Hi, > > I am a first year Med Lab Science student and am doing a project on "Natural Dyes". Has anyone here ever tried using Beet juice to stain tissues? If yes, could you please tell me something about it (like the extraction procedure, mordant used, pH and what does it stain). Is there any other Natural dye you would suggest me to use? > > Dev > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Mar 24 12:53:12 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] JB Fixative In-Reply-To: References: Message-ID: <6.0.0.22.1.20050324110353.01b364f0@gemini.msu.montana.edu> Is there someone selling this fixative under the name, JB fixative? After Jay Beckstead, this fixative was later used by Nitta et al, Cell Vision, Improved in situ immunodetection of leukocytes on paraffin embedded mouse spleen 4(1):73-80, 1997 (a defunct journal) to avoid any kind of formalin fixation for murine CD markers. Nitta never called it JB, but I really like this acronym/name!!! This is an excellent publication to read as it also refers to antibodies they use, vendor, along with tissue processing and IHC method used. We repeated IHC results nicely using Alk Phos IHC method. I have not seen this fixative referred to as JB fixative. BD Pharmingen refers to and sells it under the name Zinc Fixative but do us (or have done in the past) no favors with their name for this "JB" fixative. People tend to immediately confuse it with Zinc formalin - a case of similar names but very dissimilar fixatives, particularly when formalin is NOT wanted for these murine CD markers. Maybe BD Pharmingen is looking in, and will do us a favor with a distinct renaming - JB fixative!! It is still called Zinc fixative in their online catalog although they did add "formalin free". Ho Hum! Our lab always called it ZincTRIS fixative (ZnTRIS for short) to (hopefully) not confuse it with zinc formalin, and I plan to change it to JB - so tidy! ***Nitta indicated fixation of murine tissues could be as long as 72 hours - rather than just 48 hours. Although JB/PE tissues are touted to give tissue morphology comparable to FFPE tissues the fixative can present some crunchy tissue blocks, rather dry and friable noticed at sectioning. We had to do long ice water soaking before sectioning. It gives adequate morphology but not comparable per previous comment from our experience. Also, be careful about placing too large a tissue in this fixative. If the tissue is NOT totally fixed by JB, then 70% alcohol and processing alcohols will finish the fixation for you, not ideal for IHC purposes - this happened to a colleague of mine when she tried JB fixative. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 At 07:37 AM 3/24/2005, you wrote: >Megan, >Here is the "recipe" for the JB fixative which we have used for fixation >of mouse tissues in which we wanted to detect CD3, CD4 and CD8. > >JB Fixative > >0.1 M Tris Buffer, pH 7.4 1000ml >Calcium acetate 0.5 g >Zinc Acetate 5.0 g >Zinc Chloride 5.0 g > >Mix to dissolve. The final pH will be approximately 6.8 (6.5-7.0). Do >not readjust the pH, as this will cause the zinc to come out of >solution. Small amounts of insoluable zinc oxychloride often >contaminates zinc chloride. This may result in a very small amount of >white precipitate. This will not cause any problems but can be removed >by filtering the solution. > >Fix tissue for 24-48 hours, then transfer tissues to 70% ethanol. > >Reference: >Beckstead JH. A Simple Technique for Preservation of Fixative-sensitive >Antigens in Paraffin-embedded Tissues: Addendum (Letter to the Editor). >J Histochem Cytochem 1994; 43:345 > > >Pat Greer >Centers for Disease Control and Prevention >Infectious Disease Pathology Activity >1600 Clifton Road, MS G-32 >Atlanta, GA 30333 > > >I require some information regarding these fixatives. If anyone out in >Histonetland is is using any of these fixatives or has a reference for >me , I would be very happy and thankful. > >CBA formalin >JB fixative >Excell > >Thank you for your help in advance with my query. > >HAPPY EASTER and HAPPY HOLIDAYS > >Zenobia Haffajee >Hunter Area New England Health >IHC dept >Newcastle >Australia > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From petepath <@t> yahoo.com Thu Mar 24 12:55:15 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] RE: which new cryostat Message-ID: <20050324185515.3958.qmail@web30404.mail.mud.yahoo.com> Dear Geoff, We have had 4 Leica 1850s at HUMC in a very busy surg path practice for about 10 years. I originally chose this cryostat, because it was the most comfortable and easy for me to cut using brush technique. My hands were not twisted in a painful knot. I felt the stage was the most accessable for retrieving. I also found the controls very easy and in no time I could function at my usual pace. It seemed to have a very solid microtome and was considerably more spacious than our old tissue teks. I compared the Leica to the other brands at this clinical level and found this to be my choice for all of the above reasons. . I am very happy with the choice I made. They have been reliable work horses and have required very little more than it was the routine maintenence. I have been quite satisfied with the quality of the preparations I am capable of making and I find the work space accomodating. You have 360 degree chuck rotation and a single knob for X-Y axis adjustment. I have kept up with most of the new designs of the other brands. I would still buy another 1850 tomorrow. Trust me none of them are perfect, or even close, but of what is out there this is my choice. I must mention that I do have a relationship with Leica for a about a year. They have been helping me promote an embedding system that I developed while practicing in these cryostats. I thought about whether to write this at all, but everything I have to say came long before. I have had great success in a very busy practice with these cryostats I am happy to recommend them. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From JMacDonald <@t> mtsac.edu Thu Mar 24 13:27:52 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] H&E slides for HT practical exam In-Reply-To: <1111674123.4242cd0bc4955@imp.vet.upenn.edu> Message-ID: Pam, When a pathologist reviews slides he is considering whether or not a slide is diagnositic. The ASCP is looking for the best slide possible. Many pathologists that review slides do not look at them with the ASCP criteria in front of them, but scan the slide to look at the overall picture. The pathologist may not be aware that epithelium must cover one entire surface, or that the minimum size requirements. When I review slides with my students we go over the entire slide looking for any problems. Each applicant receives a complete list of the grading criteria that the ASCP will be using. Jennifer From NLemke <@t> asterand.com Thu Mar 24 13:41:57 2005 From: NLemke <@t> asterand.com (Nancy Lemke) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] CD1a Message-ID: <56E63440ABF1FC4897947D5BA4CFA44F04B2DACB@ATL1VEXC005.usdom004.tco.tc> Hello Histonetters, Do any of you have suggestions for staining Langerhans cells in FFPE skin? I have been wrestling with CD1a and have not had great success. I would cherish any words of wisdom. Thanks, Nancy Lemke Asterand, Inc. TechOne Suite 501 440 Burroughs Detroit, MI 48202 This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. From DNolan <@t> evanhospital.com Thu Mar 24 13:52:00 2005 From: DNolan <@t> evanhospital.com (Donna M. Nolan) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Tissue Tek Xpress Message-ID: <2C7B7A4D7DE62A46BC2EFB70D3ABF9326A492A@EVANXSCL.evanhospital.net> Has any one purchased the Tissue Tek Xpress processor? What on the pros and cons of this instrument? We routinely process overnight, in the morning block, cut etc. What is the workflow in a lab with this instrument? From la.sebree <@t> hosp.wisc.edu Thu Mar 24 14:10:07 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] CD1a Message-ID: Nancy, We use Biocare's CD1a at 1:10 with 3" HIER in Biocare's decloaking chamber in Biocare's BORG retrieval buffer and incubate for 32" OR use mild CC1 cell conditioner on Ventana's Benchmark or Benchmark XT immunostainers again with a 32" incubation. Hope this helps. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nancy Lemke Sent: Thursday, March 24, 2005 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD1a Hello Histonetters, Do any of you have suggestions for staining Langerhans cells in FFPE skin? I have been wrestling with CD1a and have not had great success. I would cherish any words of wisdom. Thanks, Nancy Lemke Asterand, Inc. TechOne Suite 501 440 Burroughs Detroit, MI 48202 This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ljones <@t> pathology.umsmed.edu Thu Mar 24 14:22:49 2005 From: ljones <@t> pathology.umsmed.edu (Linda Jones) Date: Fri Sep 16 15:24:49 2005 Subject: Fwd: [Histonet] Tissue Tek Xpress Message-ID: I would like to know about the Tissue Tek Xpress? Linda Harper-Jones BS.,HT/ HTL(ASCP) University of Mississippi Medical Center Department of Pathology Chief Histotechnologist Supervisor (601) 984-1576 (601) 984-4968 Fax ljones@pathology.umsmed.edu >>> "Donna M. Nolan" 3/24/2005 1:52:00 PM >>> Has any one purchased the Tissue Tek Xpress processor? What on the pros and cons of this instrument? We routinely process overnight, in the morning block, cut etc. What is the workflow in a lab with this instrument? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Mar 24 14:33:12 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] CD1a Message-ID: Hi Linda: A 1:10 dilution sounds "very expensive"! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sebree Linda A." 03/24/05 03:10PM >>> Nancy, We use Biocare's CD1a at 1:10 with 3" HIER in Biocare's decloaking chamber in Biocare's BORG retrieval buffer and incubate for 32" OR use mild CC1 cell conditioner on Ventana's Benchmark or Benchmark XT immunostainers again with a 32" incubation. Hope this helps. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nancy Lemke Sent: Thursday, March 24, 2005 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD1a Hello Histonetters, Do any of you have suggestions for staining Langerhans cells in FFPE skin? I have been wrestling with CD1a and have not had great success. I would cherish any words of wisdom. Thanks, Nancy Lemke Asterand, Inc. TechOne Suite 501 440 Burroughs Detroit, MI 48202 This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Thu Mar 24 14:43:11 2005 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] CD1a Message-ID: <5CB39BCA5724F349BCB748675C6CA1A202C7569E@SJMEMXMB02.stjude.sjcrh.local> We use Ventana CD1a on the Benchmark and ES and it always works great. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Lemke Sent: Thursday, March 24, 2005 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD1a Hello Histonetters, Do any of you have suggestions for staining Langerhans cells in FFPE skin? I have been wrestling with CD1a and have not had great success. I would cherish any words of wisdom. Thanks, Nancy Lemke Asterand, Inc. TechOne Suite 501 440 Burroughs Detroit, MI 48202 This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Thu Mar 24 15:00:38 2005 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] JB Fixative In-Reply-To: <6.0.0.22.1.20050324110353.01b364f0@gemini.msu.montana.edu> References: <6.0.0.22.1.20050324110353.01b364f0@gemini.msu.montana.edu> Message-ID: <42432A76.9080601@bitstream.net> Gayle Callis wrote: > Is there someone selling this fixative under the name, JB fixative? > .... > > Nitta never called it JB, but I really like this acronym/name!!! ... > Not to be critical Gayle & others, but... I think the correct name is J&B ...as in J&B Rare Scotch Whiskey.... And I agree... it is a fine fixative indeed. After a fifth of the stuff last New Year's Eve, I was pretty well fixed for the next three days. ~ Ford Ford M. Royer, MT(ASCP) Minneapolis, MN From emerald_lake77 <@t> yahoo.com Thu Mar 24 15:40:53 2005 From: emerald_lake77 <@t> yahoo.com (- -) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] B5 Fixative - postfixation?? Message-ID: <20050324214053.59086.qmail@web42303.mail.yahoo.com> Hello all, Regarding B5 fixative (not the substitutes), does it have the same effect on nuclear staining after samples has been fixed in 10% NBF and processed? The question was posed to me during a meeting and honestly, I have never really known. I know before even fixing, the tissue can be dropped into B5 for a few hours and then switched into 10% NBF to hold until processing. But for sections that have just been processed routinely and brought to water, can one place them into B5 and hope to get better than usual nuclear staining?? Thank you in advance for your assistance. --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From pruegg <@t> ihctech.net Thu Mar 24 15:46:06 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Colorado meeting April 22 & 23 Message-ID: <200503242146.j2OLk3df023196@chip.viawest.net> DON'T MISS OUT ON ANOTHER GREAT MEETING IN THE BEAUTIFUL COLORADO MOUNTAINS! 2005 Colorado Society of Histotechnology & NSH Region VII Meeting www.coloradohisto.org/2005) Dates: April 22 & 23, 2005 Location: ----------------- Beaver Run Resort 620 Village Rd. Breckenridge, CO 80424-2115 Ph: (800) 525-2253 (970) 453-6000 Fx: (970) 453-4284 www.beaverrun.com Registration: -------------------- Friday: 12:00 - 1:00 Saturday: 7:00 - 8:00 Exhibitors: -------------------- Friday: 9:30 - 6:00 Saturday: 9:30 - 5:00 Schedule: Friday, April 23rd ----------------------------- 12:00 - 1:00 Registration 1:00 - 2:30 Class 1-A or 1-B 2:30 - 3:00 Break 3:00 - 4:30 Class 2-A or 2-B 4:30 - 6:00 Social Saturday, April 24th -------------------- 7:00 - 8:00 Registration and Breakfast 8:00 - 9:30 Class 3-A or 3-B 9:30 - 10:00 Break 10:00 -11:30 Class 4-A or 4-C 11:30 - 1:00 Lunch and CSH Business Meeting 1:00 - 2:30 Class 5-A or 5-B 2:30 - 3:00 Break 3:00 - 4:30 Class 6-A or 6-B 4:30 - 5:00 Vendo drawing Deadlines: ---------- Hotel Reservations - March 8, 2005 CSH Pre-registration - April 2, 2005 **************************************************************************** SESSION 1: FRIDAY AFTERNOON, APRIL 22, 2005 1:00 PM - 2:30 PM, Presentations 1-A. The History of Automation in Histology Mr. H. Skip Brown, B.A., HT (ASCP) Lab Management Consultants - St. Louis, MO CEU credit hours: 1.5 ---------------------------------------------------------------------------- 1-B. Update for Markers in Breast Cancer Dr. Meenakshi Singh, M.D. Associate Director of Surgical Pathology, UCHSC - Aurora, CO This presentation will cover the clinical relevance of estrogen receptor, progesterone receptor and Her 2neu analysis by immunohistochemistry, image analysis and fluorescent in situ hybridization studies in invasive breast cancer. The expression profile of a patient's tumor for these markers is used in tailoring treatment decisions and assessing prognosis for individual patients. Recently the scope of ER/PR IHC has been expanded to also being incorporate these into the clinical work up of cases of non-invasive breast cancer (ductal carcinoma in situ). The potential uses of newer markers in breast cancer and metastatic breast cancer will also be discussed; these shall include clinical research done in Dr. Singh's laboratory at the University of Colorado Health Sciences Center in recent years. CEU credit hours: 1.5 **************************************************************************** 3:00 PM - 4:30 PM, Presentations 2-A. The Challenges of Change Mr. H. Skip Brown, B.A., HT (ASCP) Lab Management Consultants - St. Louis, MO CEU credit hours: 1.5 ---------------------------------------------------------------------------- 2-B. Changes in the QIHC Ms. Patsy Ruegg, HT(ASCP) QIHC IHCtech, FitzSimmons Bioscience Park - Aurora, CO., www.ihctech.net This presentation can help prepare potential examinees for the new written format for the QIHC exam by going over the projects required in the past and their application to the written questions anticipated on the new exam. The subject categories asked about on this written exam will be covered in this presentation. A list of IHC theory books and handbooks to use to prepare for the exam will be provided. CEU credit hours: 1.5 **************************************************************************** SESSION 2: SATURDAY MORNING, APRIL 23, 2005 8:00 AM - 9:30 AM, Presentations 3-A. Contrasting Sharp and Blunt Force Trauma Dr. Stephen Cina, M.D. Weld County Coroner Pathologist, Mckee Medical Center - Loveland, CO Following this presentation, the participant will be able to: 1. Discriminate between sharp force and blunt force injuries. 2. Recognize the significance of "pattern injuries." 3. Associate the types of injuries caused by various weapons types. A cutaneous injury may be the result of blunt or sharp force trauma. Even to the trained eye, it may be difficult to discriminate between lacerations, cuts, stabs or gunshot wounds. This presentation will focus on the diagnostic features of blunt force and sharp force injuries. Mechanisms of these types of injuries will be discussed. Due to the nature of the topic, this presentation will utilize many graphic images to illustrate salient points. CEU credit hours: 1.5 ---------------------------------------------------------------------------- 3-B. Localization of Mycobacterium tuberculosis in diseased tissue utilizing in situ hybridization in combination with immunohistochemistry Ms. Liz Chlipala, B.S., HTL (ASCP) QIHC, Premier Lab, LLC. - Boulder, CO, www.premierhistology.com Ms. Allison L. St. Amand, M.S., University of Colorado at Boulder Diagnosis of tuberculosis generally relies on a combination of acid fast staining of histological specimens, and culture methods. AFB staining is unable to speciate different mycobacteria. In addition mycobacterial acid fastness can be partly or completely lost at some stages of growth of the organisms. Alternatively in situ hybridization can be used to detect bacterial distribution and morphology in the context of histopathology of the tissue. In situ hybridization utilizing 16S ribosomal RNA oligonulceotide probes allows for rapid speciation of mycobacteria. This technique used in combination with immunohistochemistry allows for better understanding of the disease process. This lecture will demonstrate the use of ISH, IHC, and IF in animal models of Mycobacterium tuberculosis infections. CEU credit hours: 1.5 SESSION 2: SATURDAY MORNING, APRIL 23, 2005 10:00 AM - 11:30 AM, Presentations 4-A. Theory and Practice of Microwave Technology Mr. H. Skip Brown, B.A., HT (ASCP) Lab Management Consultants - St. Louis, MO CEU credit hours: 1.5 ---------------------------------------------------------------------------- ---- 4-B. The Human Genome Project Ms. Donna Lawson, HT (ASCP) QIHC Technical Trainer, Ventana Medical Systems Tuscon, AZ, www.ventanamed.com The Human Genome Project was officially started in 1990; the involvement became a world wide project of scientists and organizations. The goal was to generate a high-quality reference DNA sequence for the human genome's 3 billion base pairs and to identify all human genes. The project completed in 2003. Even though it was a federally funded project there was great effort to make the information discovered available to the private sector. Grants were funded for innovative research which in turn created a multibillion dollar biotechnology industry. What does this all mean to the medical profession? Because of the project a new world of drug discovery, genetic testing, and bioethics were created. This workshop will cover the inception of the Project and the terminology of genetics in a simple format. It will also discuss the areas the project has touch in medicine, agriculture and industry. The questions of bioethics that arose during the project will be covered along with some possible answers. CEU credit hours: 1.5 SESSION 3: SATURDAY AFTERNOON, APRIL 23, 2005 ============================================= 1:00 PM - 2:30 PM, Presentations 5-A. Great Cases from Small Places (I) Dr. Thomas Haas, D.O. Pathologist, Mercy Health System - Janesville, WI In many hospitals, there is no communication or connection between the work done by histotechnologists, the interpretations and recommendations made by pathologists, and the final diagnosis and treatment of the patient by clinicians. Oftentimes, the histotechnologist is not encouraged to attend "Tumor Boards" or other peer-review situations involving patient care. This can be frustrating to those involved in the "behind the scenes" work of the laboratory, and can tend to squelch understanding, interest, and learning on the part of the histotechnologist, when no feedback or integration of "why" certain stains and investigative laboratory techniques were ordered. This seminar will explore a number of different pathology cases and their final diagnoses, beginning with patient information, progressing through the reasoning behind ordering special stains, immunohistochemistry, flow cytometry, and other pertinent laboratory methods, with an explanation of the disease process, diagnostic methods, and final treatment of the patient. These cases range from those seen everyday in the hospital pathology laboratory, to those requiring outside expert interpretation. CEU credit hours: 1.5 ---------------------------------------------------------------------------- ---- 5-B. Laser Capture Microdissection (LCM) - HT Perspective Ms. Ann Bennett Lossing, Field Application Scientist, Arcturus, Inc. - Mountain View, CA, www.arctur.com Gene Expression profiling of specific cell types in both frozen and formalin-fixed breast cancer biopsy samples. Microarrays are valuable tools for studying normal and induced variations in gene expression. Microgram amounts of total RNA are required for target preparation for most microarray platforms. Consequently, whole tissue biopsies are typically used for these studies. However, distinct differences have been shown between gene expression data obtained from whole tissue biopsies, which are essentially mixed cell populations, and that obtained from homogenous populations of cells. Microdissection enables the profiling of native expression levels of thousands of genes, in a selected cell population. In this presentation we will show how microdissection, combined with RNA amplification to produce the amounts of aRNA needed for microarray analysis, have allowed us to generate expression profiles in specific cell populations obtained from breast cancer biopsy samples. As a result of recent technological advances, we are able to isolate RNA from both frozen and formalin fixed tissue samples, and used this RNA to obtain expression profiles. These highly reproducible expression profiles have been used to generate molecular signatures for different stages of breast cancer using frozen biopsy tissues and microarray analysis. Our presentation will focus on the importance of developing histological methods for providing excellent visualization of cell types, while at the same time, preserving preserve RNA quality. Topics to be discussed:- Laser Capture Microdissection Technology Microgenomics Reagent Systems Paradise T Formalin-Fixed Tissue - Expression Analysis System Application Review - Arcturus LCM-based Publications (over 550 available to date!) CEU credit hours: 1.5 3:00 PM - 4:30 PM, Presentations 6-A. Great Cases from Small Places (II) Dr. Thomas Haas, D.O. Pathologist, Mercy Health System - Janesville, WI See presentation 5-A for abstract CEU credit hours: 1.5 ---------------------------------------------------------------------------- ---- 6-B. Dewax, Rehydration, and Antigen Retrieval the Easy Way Ms. Patricia Piatti & Mr. Allen Younger Biogenex, San Ramon, CA How do you conduct Dewax, Rehydration, and Antigen Retrieval for your formalin-fixed, paraffin-embedded tissue sections? Do you baby-sit every tedious step of toxic xylene and alcohol dewax and rehydration pretreatment? Do you have difficulty in preserving tissue sample morphology and obtaining consistent results when you using traditional pressure cookers, water baths, and microwaves for antigen retrieval? Do you need a lot of guess work and training? Now, with BioGenex's EZ Retrieval System, the sample pretreatment has never been as easy as today. EZ RetrieverT allows you to shorten multiple steps of traditional tissue sample pretreatment from 85min to less than 30 mins. With a throughput as high as 96 slides/run, you may even save more than 4 hours of work and you can almost walk away from the whole process. With the ease of use digital interface and temperature probe, you can standardize the procedure with little training. By using eco-friendly ZEN-AR solutions that have been optimized for each antibody, you can preserves the tissue morphology and gain high quality and reproducible results. To learn more about the ZEN Retrieval System, please join the ZEN Retrieval System work shop that will be held in the following locations or visit our website at www.biogenex.com. CEU credit hours: 1.5 **************************************************************************** Online registration and additonal information regarding the meeting can be found at www.coloradohisto.org/2005 From ploykasek <@t> phenopath.com Thu Mar 24 15:52:32 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] CD1a In-Reply-To: <56E63440ABF1FC4897947D5BA4CFA44F04B2DACB@ATL1VEXC005.usdom004.tco.tc> Message-ID: We are using CD1a clone 010 from Immunotech. We use a heat citrate antigen retrieval method. With a polymer detection system. The primary antibody is titered at 1:25 (this is sold as a prediluted antibody). Please contact me if you need more info. Patti Loykasek PhenoPath Laboratories Hello Histonetters, > > Do any of you have suggestions for staining Langerhans cells in FFPE > skin? I have been wrestling with CD1a and have not had great success. > I would cherish any words of wisdom. > > Thanks, > > Nancy Lemke > > Asterand, Inc. > > TechOne Suite 501 > > 440 Burroughs > > Detroit, MI 48202 > > > > This e-mail message, together with any attachments, contains information > belonging to Asterand, Inc. that may be confidential, proprietary, > copyrighted and/or legally protected or privileged, and is intended for > the sole use of the individual or entity named on this message. If you > are not the intended recipient, and have received this e-mail message in > error, please immediately return this message to its original sender and > permanently delete it from your electronic and paper records. Thank > You. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Thu Mar 24 16:23:06 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Re:Gloves ..... a question Message-ID: Very clever!!! Robyn OHSU >>> "Johnson, Kevin" 03/24/05 9:15 AM >>> I label my slides by first placing them in a cardboard 20-slide folder with the cover removed, then placing an open folder over the slides, exposing only the frosted portion. The writing hand rests on the cardboard rather than on the slide, and a single finger on the edge of the frosted portion stabilizes the slide for labeling. Kevin Johnson University of Miami Diabetes Research Institute Miami, FL > -----Original Message----- > From: John Kiernan [mailto:jkiernan@uwo.ca] > Sent: Wednesday, March 23, 2005 5:43 PM > To: Histonet > Subject: Re: [Histonet] Re:Gloves ..... a question > > "Marshall Terry Dr, Consultant Histopathologist" wrote: > > ... Slides are invariably labelled with the writing right > > way up, frosted end pointing North. This means the fingers > > and heel of the hand rest on the slide. That is the > > source of the squames. > > Now that's interesting. I've only ever written > on the frosted end with the section end pointing > north. Why would anyone want to do it the other > way round? > > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Thu Mar 24 17:04:19 2005 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] JB Fixative Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CB5F@usca0082k08.labvision.apogent.com> Ford, that reminds me of the"alcohol fixation" seminar I took at the NSH meeting in Las Vegas many years ago...! Tim Morken Not to be critical Gayle & others, but... I think the correct name is J&B ...as in J&B Rare Scotch Whiskey.... And I agree... it is a fine fixative indeed. After a fifth of the stuff last New Year's Eve, I was pretty well fixed for the next three days. ~ Ford Ford M. Royer, MT(ASCP) Minneapolis, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Thu Mar 24 18:47:10 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Tissue Tek Xpress Message-ID: While our lab does not have one, I had the pleasure of visiting the lab in Miami where the unit was created. This is a huge teaching hospital and they have multiple residents constantly grossing all day. With this intrument they were able to load specimens every 15 minutes (there are 4, 15 minute solutions) and techs were constantly removing processed specimens, embedding, cutting, and passing along stained slides to the pathologists throughout the day.The pathologists were then able to get reports out steadily all day long. Turn-around time was amazing, the need for a night shift was removed, and the quality was amazing. Jeanine Bartlett CDC/Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Donna M. Nolan Sent: Thu 3/24/2005 2:52 PM To: Histonet Cc: Subject: [Histonet] Tissue Tek Xpress Has any one purchased the Tissue Tek Xpress processor? What on the pros and cons of this instrument? We routinely process overnight, in the morning block, cut etc. What is the workflow in a lab with this instrument? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.bublava <@t> meduniwien.ac.at Fri Mar 25 01:26:02 2005 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Alcian Green Dye References: <20050324145137.83325.qmail@web51005.mail.yahoo.com> Message-ID: <002601c5310b$e8e5e930$dd01a8c0@GERICHTS9XOZZ8> Its a far way, but at http://www.chroma.de/ you can find it. In German it is called Alciangr?n - they have 3BX and 2GX, but they do have an english catalogue too. They are specialiced in dyes and have nearly everything Hope that helps Barbara ----- Original Message ----- From: "Histology SLU" To: Sent: Thursday, March 24, 2005 3:51 PM Subject: [Histonet] Alcian Green Dye > Hello All: > > Just got off of the phone with sigma and they no longer carry alcian green and do not have another source to suggest. Can anyone out there share their source that is not sigma? Thanks, in advance. Have a blessed Easter. > > Susan > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Mar 25 02:43:48 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Alcian Green Dye References: <20050324145137.83325.qmail@web51005.mail.yahoo.com> Message-ID: <007d01c53116$c46ac3e0$b4d1d445@domainnotset.invalid> I'm going to guess - probably not. It was explained to me (long time ago) that Alcian green is a mixture of Alcian yellow and Alcian blue. Since Alcian yellow is no longer being made, then I would guess that Alcian green will no longer be made either. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Histology SLU" To: Sent: Thursday, March 24, 2005 9:51 AM Subject: [Histonet] Alcian Green Dye > Hello All: > > Just got off of the phone with sigma and they no longer carry alcian green and do not have another source to suggest. Can anyone out there share their source that is not sigma? Thanks, in advance. Have a blessed Easter. > > Susan > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Mar 25 02:46:01 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Wright giemsa on bone marrow core biopsies... References: <992899E9EC268548AB8DDE246AF88473055F4F0A@PAHEX01.uphs.upenn.edu> Message-ID: <008601c53117$12fce6a0$b4d1d445@domainnotset.invalid> Smears can be stained with Wright Giemsa or with Leishmann Giemsa. Fixed tissue require a different formulation, such as Wolbach Giemsa or Jenner Giemsa. I've included our version of Jenner Giemsa. JENNER GIEMSA REAGENTS: STOCK JENNER SOLUTION Jenner dye 1.0 g Absolute methanol (CH3OH) 400.0 mL Dissolve together. Store at room temperature. Stable for months. WORKING JENNER SOLUTION Stock Jenner solution 25.0 mL Distilled water 25.0 mL JUST BEFORE USE, mix together. Good for 1 day only. STOCK GIEMSA SOLUTION Giemsa dye 1.0 g Glycerin (C3H8O3) 66.0 mL Absolute methanol (CH3OH) 66.0 mL Mix together glycerin and Giemsa dye. Place in 60o C. oven for 30 minutes to 2 hours, until dissolved. Add methanol and mix. Store at room temperature in a closed, dark brown bottle. Keep away from heat and sunlight, which will shorten the life of the solution. Stable for about 1 year. WORKING GIEMSA SOLUTIONS Stock Giemsa solution 1.5 mL Distilled water 50.0 mL JUST BEFORE USE, mix together. Good for one day only. 0.5% ACETIC ACID WATER Acetic acid, conc. (CH3COOH) 0.5 mL Distilled water 99.5 mL If this stain is done infrequently, make JUST BEFORE USE, mix together. Stable for one day only. PROCEDURE - Jenner-Giemsa: 1. Deparaffinize and hydrate slides through graded alcohol to distilled water. 2. Place in 2 changes of absolute methanol 3 minutes each 3. Stain in WORKING Jenner solution 6 minutes 4. Transfer directly into WORKING Giemsa solution 45 minutes THE FOLLOWING STEPS WILL DONE ONE SLIDE AT A TIME: 5. Rinse quickly in distilled water 3 seconds 6. Differentiate in 0.5% acetic acid, until can begin to see chromatin pattern in the nuclei. Check with microscope 1-3 seconds 7. Rinse in distilled water 2-3 seconds 8. Complete differentiation of chromatin pattern until crisp and clear in 2 changes of 95% ethanol. Check with microscope 1-10 seconds each 9. Dehydrate in absolute ethanol, 2 changes 5-10 seconds each 10. Clear in xylene 10 seconds each 11. Coverslip using a synthetic mounting media. RESULTS: Nuclei - blue Cytoplasm, collagen, muscle, bone spicules - pink/blue/gray Eosinophilic granules - pink Basophilic granules - blue Bacteria, parasitic protozoa cytoplasm - blue Red blood cells - yellowish-pink PROCEDURAL NOTES: 1. Chromatin material of nuclei should be readily distinguished for good differentiation. 2. If overdifferentiated, slides may be placed back into the Giemsa solution and restained. 3. When differentiating, either in acetic acid or 95% ethanol, proceed one slide at a time. ----- Original Message ----- From: "Goodwin, Diana" To: "Histonet (E-mail)" Sent: Wednesday, March 23, 2005 1:04 PM Subject: [Histonet] Wright giemsa on bone marrow core biopsies... > ...looking for a good method for tissue as opposed to smears. > > Thanks, > Diana Goodwin > Anatomic Pathology > Pennsylvania Hospital > 215-829-6532 > e-mail: goodwind@pahosp.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sakima <@t> bigpond.net.au Fri Mar 25 04:40:28 2005 From: sakima <@t> bigpond.net.au (Satoshi Akima) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] anti-neutrophil elastase antibody Message-ID: <104b44e620f24b81d1aebb665af5c8fd@bigpond.net.au> Dear all does anyone have any experience with getting the Dako anti-neutrophil elastase antibody to work in IHC using paraffin sections? If anyone would be willing to share a protocol I would be greatly indebted! Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia From hoye <@t> tarleton.edu Tue Mar 22 15:38:54 2005 From: hoye <@t> tarleton.edu (Glenda Hoye) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] H&E slides for HT practical exam References: <28509852.1111524742645.JavaMail.root@thecount.psp.pas.earthlink.net> Message-ID: <015801c52f27$8c203c40$04895fa5@tarleton.edu> Well, I'm not Peggy or Jennifer, but I think I know the answer to your quandary... The ASCP graders don't know, or care, how the slides were stained - as long as the outcome is wonderful H&E's. That's what they look for - the results, not fine details like were they all stained together. Some tissues take up stain differently from others, so staining them all together might not give the best results. Staining each one separately might be the best way to get excellent stains. As for your co-worker's comment that 'someone will know' -- the graders are experienced histotechs and pathologists, but I've never met one who had the powers to 'know' what went on in the examinee's lab with each specific slide (as to single or batch staining)! She gives us a lot of credit! ;) I hope this helps solve the dilemma in your lab, and please pass along to your co-worker my best wishes on passing the practical exam this time! Glenda Hoye (previously on the HT exam committtee) Glenda Hoye, HT(ASCP) Histotechnology Program Director Tarleton State University Fort Worth, TX 76104 817-926-1101 x234 ----- Original Message ----- From: "Mark Adam Tarango" To: Sent: Tuesday, March 22, 2005 2:52 PM Subject: [Histonet] H&E slides for HT practical exam > Hi everyone. I have a co-worker who insists that she must stain all her H&E and slides for her practical exam together at once. My supervisor and I haven't ever heard this, and I'm hoping someone can give a reply as to whether or not this is something that is required. She is conviced that if she doesn't stain them all together (her H&E slides), someone will know and fail her again. If Peggy Wenk or Jennifer MacDonald are watching, we'd like to her from you especially. > > > Thanks, > > > Mark Tarango > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ajennings <@t> unmc.edu Fri Mar 25 07:54:33 2005 From: ajennings <@t> unmc.edu (ajennings@unmc.edu) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] labeling slides Lefties Unite :) Message-ID: All I can say about the labeling slides issue.... Just another benefit to being left handed (if you aren?t the kind that turns their whole hand around to write) I am beginning to think left handed might need to go under "preferred" in the next job posting for a histotech (teasing) btw this is said tongue in cheek because for so many years I 'made due' with things that were designed for righties so it is kind of nice to see I chose a career that seems to subliminally be made for lefties From pathrm35 <@t> adelphia.net Fri Mar 25 08:24:55 2005 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Fed Ex transportation of tissue biopsies Message-ID: <3138834.1111760695267.JavaMail.root@web5.mail.adelphia.net> Fellow tech's, I was wondering how some of you receive and transport tissue biopsies using outside delivery companies?(in particular Fed Ex). Do you use any special packaging and or just what they supply? Thanks, Ron From rgrow <@t> bmnet.com Fri Mar 25 08:38:02 2005 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Automated Microtomes Message-ID: Are there Histotechs who actually use automated microtomes daily? Not the ones you have purchased, then seldom use or use in a manual mode. I need real-time guidance from techs who use them daily and what likes/dislikes you have with the particular brand bought. Any response is appreciated. Thanks. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax From RizoC <@t> chi.osu.edu Fri Mar 25 08:48:12 2005 From: RizoC <@t> chi.osu.edu (Rizo, Christian) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Sliding Microtomes Message-ID: <979FF5962E234F45B06CF0DB7C1AABB201FEA549@chi2k3ms01.columbuschildrens.net> Hi Histonetters, I was just curious but does anyone know any other brand of a sliding microtome other than the Leica Microtome SM2000R sliding microtome. Your help is greatly appreciated. I'll buy dinner in Columbus, OH Chris Christian M. Rizo MBA, HTL (ASCP) Manager, Anatomic Pathology Childrens Hospital 700 Childrens Drive Columbus, Ohio 43205 614.722.5465 RizoC@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From cgfields <@t> lexhealth.org Fri Mar 25 08:57:10 2005 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Automated Microtomes Message-ID: We use the Microm HM 355 S and we love them. I bought 6 of them. The automation takes the pressure off of your wrist and shoulder. We liked the Microm better than the Lieca because you can use it manual (when you need to) and automatic. If you have a tiny piece of tissue and you feel more comfortable using the manual mode you can. Also it is not as bulky as the Leica. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 (803) 936-8214 -----Original Message----- From: rgrow@bmnet.com [mailto:rgrow@bmnet.com] Sent: Friday, March 25, 2005 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Microtomes Are there Histotechs who actually use automated microtomes daily? Not the ones you have purchased, then seldom use or use in a manual mode. I need real-time guidance from techs who use them daily and what likes/dislikes you have with the particular brand bought. Any response is appreciated. Thanks. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From Don.Birgerson <@t> leica-microsystems.com Fri Mar 25 09:10:55 2005 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Sliding Microtomes Message-ID: Hi Chris, Leica-Microsystems has discontinued our AO 860 and our Reichert OME but we still have the SM2400 Sledge microtome and the SM2500 Poly Cut. The SM2500 is capable of large ( 8" x 10" ) blocks with a automatic cutting range of 1 micron to 999 micron. Using those model numbers you can search these easily on our web site. If you have further questions , pleas call. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 "Rizo, Christian" To: Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] Sliding Microtomes western.edu 03/25/2005 08:48 AM Hi Histonetters, I was just curious but does anyone know any other brand of a sliding microtome other than the Leica Microtome SM2000R sliding microtome. Your help is greatly appreciated. I'll buy dinner in Columbus, OH Chris Christian M. Rizo MBA, HTL (ASCP) Manager, Anatomic Pathology Childrens Hospital 700 Childrens Drive Columbus, Ohio 43205 614.722.5465 RizoC@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From s.liabeuf <@t> wanadoo.fr Fri Mar 25 09:28:05 2005 From: s.liabeuf <@t> wanadoo.fr (Sylvie LIABEUF) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] (no subject) Message-ID: <30187791.1111764485084.JavaMail.www@wwinf0102> Hello, Do anyone know if agarose used for vibratome section is a problem for immunostaining? thank you very much for your help sylvie From Sue.Kapoor <@t> uhsi.org Fri Mar 25 09:35:52 2005 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Energy Beam microwave fixation Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0AB@khmcexch.uhsi.org> Hi, is anyone using an Enegy Beam microwave to fix fatty tissue. I found an article in the AJCP that says for fixation, to microwave for 10 minutes at 70 degrees in formalin. Although it says all tissues should be nuked like this, I'm trying to find something that works well for really fatty tissue. thanks in advance, Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 From NMargaryan <@t> childrensmemorial.org Fri Mar 25 09:44:58 2005 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] (no subject) Message-ID: <63B8B599DE283148B92E83C78B32C15D8B4B4F@cmhexbe2.childrensmemorial.org> Other question for vibratome: I have to do vibratome sections on frozen tissue- mouse breast with skin. Tissue defrosting during the vibritiming and looks like fresh tissue. It is very hard to cut. Can you give me any recommendation about frequency, amplitude and speed? I am going to make 4-10 ? think sections. Any suggestion will be appreciate, Naira -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvie LIABEUF Sent: Friday, March 25, 2005 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello, Do anyone know if agarose used for vibratome section is a problem for immunostaining? thank you very much for your help sylvie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. From liz <@t> premierlab.com Fri Mar 25 10:29:23 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] (no subject) In-Reply-To: <63B8B599DE283148B92E83C78B32C15D8B4B4F@cmhexbe2.childrensmemorial.org> Message-ID: <000001c53157$cdddd430$76d48a80@AMY> Sylvie We frequently embed our tissue in agarose for vibratome sections. We cut 30-50 micron sections of fixed mouse spleen and liver. After the sectioning you can carefully remove the agarose that is around the tissue. We then stain the 50 micron sections free floating for IF and evaluate them with the confocal microscope. My question to Naira is why are you trying to cut 4 - 10 micron frozen sections on a vibratome, why are you not cutting the frozen sections in a cryostat. The thinnest we can cut on the vibratome we have here is 30 microns. Sectioning in a cryostat would be your best bet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, March 25, 2005 8:45 AM To: s.liabeuf@wanadoo.fr; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Other question for vibratome: I have to do vibratome sections on frozen tissue- mouse breast with skin. Tissue defrosting during the vibritiming and looks like fresh tissue. It is very hard to cut. Can you give me any recommendation about frequency, amplitude and speed? I am going to make 4-10 ? think sections. Any suggestion will be appreciate, Naira -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvie LIABEUF Sent: Friday, March 25, 2005 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello, Do anyone know if agarose used for vibratome section is a problem for immunostaining? thank you very much for your help sylvie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. From liz <@t> premierlab.com Fri Mar 25 10:32:54 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:49 2005 Subject: [Histonet] Fed Ex transportation of tissue biopsies In-Reply-To: <3138834.1111760695267.JavaMail.root@web5.mail.adelphia.net> Message-ID: <000101c53158$4bed4090$76d48a80@AMY> We receive most of our samples via fed ex since we are contract lab. The specimens are usually doubled wrapped in plastic bags with some absorbent material packed around them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pathrm35@adelphia.net Sent: Friday, March 25, 2005 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fed Ex transportation of tissue biopsies Fellow tech's, I was wondering how some of you receive and transport tissue biopsies using outside delivery companies?(in particular Fed Ex). Do you use any special packaging and or just what they supply? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Rush <@t> sjmcmn.org Fri Mar 25 10:36:52 2005 From: Joyce.Rush <@t> sjmcmn.org (Rush, Joyce) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] GYN Report Disclaimer Message-ID: <2337362E8548BE4C85EE5DD5D526B08519422C@sjw3smail2.SJMCMN.ORG> I know that most of you are Histology folks but hope that you will pass this on to any clinical Cytology co-workers. We are going from JCAHO to CAP for our surveys and need to add a report disclaimer to our GYN reports. I'd appreciate as many samples as I can get so I don't have to reinvent the wheel. Thanks so much! Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 From victor <@t> pathology.washington.edu Fri Mar 25 10:43:39 2005 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] GYN Report Disclaimer In-Reply-To: <2337362E8548BE4C85EE5DD5D526B08519422C@sjw3smail2.SJMCMN.ORG> References: <2337362E8548BE4C85EE5DD5D526B08519422C@sjw3smail2.SJMCMN.ORG> Message-ID: <42443FBB.6060701@pathology.washington.edu> We are JCAHO and CAP accreditted and have no disclaimer on our reports. Is there something new that we need to be aware of? Victor Rush, Joyce wrote: >I know that most of you are Histology folks but hope that you will pass this on to any clinical Cytology co-workers. We are going from JCAHO to CAP for our surveys and need to add a report disclaimer to our GYN reports. I'd appreciate as many samples as I can get so I don't have to reinvent the wheel. > >Thanks so much! > >Joyce > >Joyce A. Rush, BS, MT(ASCP) >Laboratory Manager >St Joseph's Medical Center >523 North Third Street >Brainerd, MN 56401 >Office:218-828-7500 Fax: 218-828-7510 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From sharon.osborn <@t> dnax.org Fri Mar 25 10:59:29 2005 From: sharon.osborn <@t> dnax.org (Osborn, Sharon) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] RE: Sakura Xpress Message-ID: <29B25753F6B1D51196110002A589D44402397F2C@PALMSG30.us.schp.com> Recently, I visited Good Samuel Hospital in Portland, OR. where they have the Sakura Xpress. The pathologists state it needs tweaking for not all the tissues are fully fixed or processed during the cycles. They did state that the training to cut the tissues is very precise and necessary for good results. Some of the pathologists do not wish to change their ways of grossing so that could present a problem. It may also need the times adjusted although Sakura has worked out times in the Florida test pilot. Their rationale for getting this was histotech shortage to do the work so needing to have more efficient methods/means of production. The impression I have is it may take a commitment of time to do the training, adjusting (of equipment and people attitudes) and experimentation to get the instrument doing what it is designed optimally to do. If you are considering this equipment, it is imperative that your pathologists or PA or whomever is grossing (possibly all of them) are onboard the commitment. These are the crucial ones to getting the optimal processing through the machine. Plus, there is a second component that does the automatic sectioning of the blocks--this would definitely require the grossing personnel to be diligent in cutting and placing the tissue into the cassette for it is WYSIWYG--no chance to change it later. I remember that 20 years ago I made a statement to the effect that embedding and cutting of tissues could not be automated. Now, there is a machine that is doing pretty much that! Cheers! so sharon osborn DNAX Palo Alto, CA Message: 13 Date: Thu, 24 Mar 2005 14:22:49 -0600 From: "Linda Jones" Subject: Fwd: [Histonet] Tissue Tek Xpress To: Message-ID: Content-Type: text/plain; charset=US-ASCII I would like to know about the Tissue Tek Xpress? Linda Harper-Jones BS.,HT/ HTL(ASCP) University of Mississippi Medical Center Department of Pathology Chief Histotechnologist Supervisor (601) 984-1576 (601) 984-4968 Fax ljones@pathology.umsmed.edu >>> "Donna M. Nolan" 3/24/2005 1:52:00 PM >>> Has any one purchased the Tissue Tek Xpress processor? What on the pros and cons of this instrument? We routinely process overnight, in the morning block, cut etc. What is the workflow in a lab with this instrument? ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From BriggsK <@t> drmc.drhsi.org Fri Mar 25 11:01:14 2005 From: BriggsK <@t> drmc.drhsi.org (Kevin Briggs) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] GYN Report Disclaimer Message-ID: <7C1D5BE1ADF06E488AEB7A6247214A86015FB38C@HORNET.drmc.drhsi.org> The CAP Checklist item is as follows: CYP.07582 Phase I Is there a policy to educate providers of cervicovaginal specimens that the Pap test is a screening test for cervical cancer with inherent false negative results? NOTE: The preferred mechanism is an educational note on all negative Pap test reports. Other mechanisms include sending periodic educational information to providers, conference presentations, etc. Wherefore, the educational note, though the simplest, is only one of many ways to satisfy this requirement. The note attached to our GYN reports reads: "The gynecological Pap test is a screening test designed to aid in the detection of cervical cancer and its precursors. Interpretation of the Pap test should not be used as the sole means of detecting cervical cancer." I hope this helps. Kevin D. Briggs, MS, CT(ASCP) Team Leader- Cytopathology/ Histopathology Services Danville Regional Medical Center 142 South Main Street Danville, VA 24541 Telephone: (434) 799-4470 ext.5451 Fax: (434) 799-2118 E-mail: briggsk@drhsi.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Victor Tobias Sent: Friday, March 25, 2005 11:44 AM To: Rush, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GYN Report Disclaimer We are JCAHO and CAP accreditted and have no disclaimer on our reports. Is there something new that we need to be aware of? Victor Rush, Joyce wrote: >I know that most of you are Histology folks but hope that you will pass this on to any clinical Cytology co-workers. We are going from JCAHO to CAP for our surveys and need to add a report disclaimer to our GYN reports. I'd appreciate as many samples as I can get so I don't have to reinvent the wheel. > >Thanks so much! > >Joyce > >Joyce A. Rush, BS, MT(ASCP) >Laboratory Manager >St Joseph's Medical Center >523 North Third Street >Brainerd, MN 56401 >Office:218-828-7500 Fax: 218-828-7510 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Mar 25 11:08:06 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Automated Microtomes In-Reply-To: References: Message-ID: <6.0.0.22.1.20050325100359.01b56fa8@gemini.msu.montana.edu> Our Leica 2155 can be operated either manually or in automated mode. I am sure the newer model out is the same, 2255. At 07:57 AM 3/25/2005, you wrote: >We use the Microm HM 355 S and we love them. I bought 6 of them. The >automation takes the pressure off of your wrist and shoulder. We liked the >Microm better than the Lieca because you can use it manual (when you need >to) and automatic. If you have a tiny piece of tissue and you feel more >comfortable using the manual mode you can. Also it is not as bulky as the >Leica. > >Carole Fields, HT,ASCP >Pathology Supervisor >Lexington Medical Center >2720 Sunset Blvd. >W. Columbia, SC 29169 > >(803) 936-8214 Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From JWEEMS <@t> sjha.org Fri Mar 25 11:27:14 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] GYN Report Disclaimer Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA45749@sjhaexc02.sjha.org> We have this statement at the end of our reports: **Interpretation of Cytologic preparations is subject to both false negative and false positive results as evidenced by published data. Test results should be interpreted in context, along with history and clinical findings.** Is this what you are talking about or is there something I don't know?! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rush, Joyce Sent: Friday, March 25, 2005 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GYN Report Disclaimer I know that most of you are Histology folks but hope that you will pass this on to any clinical Cytology co-workers. We are going from JCAHO to CAP for our surveys and need to add a report disclaimer to our GYN reports. I'd appreciate as many samples as I can get so I don't have to reinvent the wheel. Thanks so much! Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From JQB7 <@t> CDC.GOV Fri Mar 25 11:46:47 2005 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] RE: Sakura Xpress Message-ID: The machine comes with a grossing board that guarantees proper grossing technique. Those pathologists that refuse to comply may simply have to use an old processor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Osborn, Sharon Sent: Fri 3/25/2005 11:59 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Subject: [Histonet] RE: Sakura Xpress Recently, I visited Good Samuel Hospital in Portland, OR. where they have the Sakura Xpress. The pathologists state it needs tweaking for not all the tissues are fully fixed or processed during the cycles. They did state that the training to cut the tissues is very precise and necessary for good results. Some of the pathologists do not wish to change their ways of grossing so that could present a problem. It may also need the times adjusted although Sakura has worked out times in the Florida test pilot. Their rationale for getting this was histotech shortage to do the work so needing to have more efficient methods/means of production. The impression I have is it may take a commitment of time to do the training, adjusting (of equipment and people attitudes) and experimentation to get the instrument doing what it is designed optimally to do. If you are considering this equipment, it is imperative that your pathologists or PA or whomever is grossing (possibly all of them) are onboard the commitment. These are the crucial ones to getting the optimal processing through the machine. Plus, there is a second component that does the automatic sectioning of the blocks--this would definitely require the grossing personnel to be diligent in cutting and placing the tissue into the cassette for it is WYSIWYG--no chance to change it later. I remember that 20 years ago I made a statement to the effect that embedding and cutting of tissues could not be automated. Now, there is a machine that is doing pretty much that! Cheers! so sharon osborn DNAX Palo Alto, CA Message: 13 Date: Thu, 24 Mar 2005 14:22:49 -0600 From: "Linda Jones" Subject: Fwd: [Histonet] Tissue Tek Xpress To: Message-ID: Content-Type: text/plain; charset=US-ASCII I would like to know about the Tissue Tek Xpress? Linda Harper-Jones BS.,HT/ HTL(ASCP) University of Mississippi Medical Center Department of Pathology Chief Histotechnologist Supervisor (601) 984-1576 (601) 984-4968 Fax ljones@pathology.umsmed.edu >>> "Donna M. Nolan" 3/24/2005 1:52:00 PM >>> Has any one purchased the Tissue Tek Xpress processor? What on the pros and cons of this instrument? We routinely process overnight, in the morning block, cut etc. What is the workflow in a lab with this instrument? ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Mar 25 12:16:19 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] B5 Fixative - postfixation?? References: <20050324214053.59086.qmail@web42303.mail.yahoo.com> Message-ID: <42445573.CB48CDE6@uwo.ca> B5 is a near-neutral fixative (pH 5.8-6.0) contrived to combine the best properties of neutral formaldehyde and mercuric chloride. Formaldehyde penetrates and stops autolysis rapidly, but its reactions with proteins are slow. Structural stabilization (hardening) takes days. Tissue thoroughly fixed (cross-linked) by formaldehyde often shows differential shrinkage artifacts - abnormal spaces around cells etc - that occur during processing into paraffin. Mercuric chloride penetrates the tissue slowly, coagulating proteins instantaneously as it moves in. The coagulation has a finer "grain" than that of proteins coagulated by alcohol or picric acid, and cytoplasmic organelles (secretory granules, and even mitochondria) are preserved well enough to be seen by light microscopy. B5 (unlike most mercuric chloride & formaldehyde mixtures) does not contain acetic acid to coagulate chromatin into textures characteristic of particular cell-types. B5 is primarily a fixative of cytoplasm that provides crisp and informative staining. It doesn't make sense to move a specimen from phosphate-buffered formaldehyde into a mercuric & formaldehyde mixture such as B5. If the tissue is already fixed (cross-linked) by formaldehyde, later reactions of structural proteins with HgCl2 are unlikely to improve anything structurally. Some staining properties might be changed for the better, but it's easier to treat hydrated slides with HgCl2 before staining. Treatment with picric acid has much the same effect, and is commonly done prior to staining sections of formaldehyde-fixed material by trichrome methods. A specimen fixed in B5 is not going to be improved by moving it into an aqueous formaldehyde solution. John Kiernan London, Canada ----------------------------------------------- An anonymous person (- - ) wrote: > > Hello all, > > Regarding B5 fixative (not the substitutes), does it have the same effect on nuclear staining after samples has been fixed in 10% NBF and processed? The question was posed to me during a meeting and honestly, I have never really known. I know before even fixing, the tissue can be dropped into B5 for a few hours and then switched into 10% NBF to hold until processing. But for sections that have just been processed routinely and brought to water, can one place them into B5 and hope to get better than usual nuclear staining?? > > Thank you in advance for your assistance. --------------------------------------------------------- From c.m.vanderloos <@t> amc.uva.nl Fri Mar 25 12:49:17 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] RE: Natural Dyes: Beet Juice Message-ID: <17fa69d17ff1f0.17ff1f017fa69d@amc.uva.nl> Hi, I am sure if it's true or not, but once I heard a story that in Louis Pasteur's days they used red Port wine for staining tissues. Definitely, more attractive than beet juice.... Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- From "Anurag Sharma" Date Thu, 24 Mar 2005 06:37:45 -0800 To Subject [Histonet] Natural Dyes: Beet Juice Hi, I am a first year Med Lab Science student and am doing a project on "Natural Dyes". Has anyone here ever tried using Beet juice to stain tissues? If yes, could you please tell me something about it (like the extraction procedure, mordant used, pH and what does it stain). Is there any other Natural dye you would suggest me to use? Dev From cwscouten <@t> myneurolab.com Fri Mar 25 13:11:41 2005 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Sliding Microtomes Message-ID: <5784D843593D874C93E9BADCB87342AB44F7A0@tpiserver03.Coretech-holdings.com> Yes, the Vibratome sliding microtome is very versatile for any size tissue. The tissue stage moves, the blade is immobile for safety http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=476301&catdesc=Histology+Equipment&CatThreeID=597&CatOneID=4&subcatdesc=Sliding+Microtomes&idsubcategory=184 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rizo, Christian Sent: Friday, March 25, 2005 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sliding Microtomes Hi Histonetters, I was just curious but does anyone know any other brand of a sliding microtome other than the Leica Microtome SM2000R sliding microtome. Your help is greatly appreciated. I'll buy dinner in Columbus, OH Chris Christian M. Rizo MBA, HTL (ASCP) Manager, Anatomic Pathology Childrens Hospital 700 Childrens Drive Columbus, Ohio 43205 614.722.5465 RizoC@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Mar 25 13:36:00 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] RE: Natural Dyes: Beet Juice Message-ID: I'd like to meet the person willing to part with his red port wine collection in the interest of science. now that is dedication! can't think of any other use for beet juice. might definitely be worth a try. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "C.M. van der Loos" 03/25/05 01:49PM >>> Hi, I am sure if it's true or not, but once I heard a story that in Louis Pasteur's days they used red Port wine for staining tissues. Definitely, more attractive than beet juice.... Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- From "Anurag Sharma" Date Thu, 24 Mar 2005 06:37:45 -0800 To Subject [Histonet] Natural Dyes: Beet Juice Hi, I am a first year Med Lab Science student and am doing a project on "Natural Dyes". Has anyone here ever tried using Beet juice to stain tissues? If yes, could you please tell me something about it (like the extraction procedure, mordant used, pH and what does it stain). Is there any other Natural dye you would suggest me to use? Dev _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mrsgbd2001 <@t> yahoo.com Fri Mar 25 14:14:05 2005 From: mrsgbd2001 <@t> yahoo.com (Gareth Davis) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Re: Sliding Microtomes Message-ID: <20050325201406.22933.qmail@web52710.mail.yahoo.com> Microm makes a great sliding microtome. Gareth __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From victor <@t> pathology.washington.edu Fri Mar 25 14:29:51 2005 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] GYN Report Disclaimer In-Reply-To: <7C1D5BE1ADF06E488AEB7A6247214A86015FB38C@HORNET.drmc.drhsi.org> References: <7C1D5BE1ADF06E488AEB7A6247214A86015FB38C@HORNET.drmc.drhsi.org> Message-ID: <424474BF.7040501@pathology.washington.edu> I stand corrected as we do have a disclaimer on our reports. I rarely look at a printed version, only an online version which doesn't show the disclaimer. Here's our disclaimer; The Pap smear is a screening test designed to aid in the detection of the premalignant and maglinant conditions of the uterine cervix. It is not a diagnostic procedure and should not be used as the sole means of detecting cervical cancer. Both false-positive and false-negative reports do occur. Victor Kevin Briggs wrote: >The CAP Checklist item is as follows: > >CYP.07582 Phase I > >Is there a policy to educate providers of cervicovaginal specimens that the Pap test is a screening test for cervical cancer with inherent false negative results? > >NOTE: The preferred mechanism is an educational note on all negative Pap test reports. Other mechanisms include sending periodic educational information to providers, conference presentations, etc. > >Wherefore, the educational note, though the simplest, is only one of many ways to satisfy this requirement. The note attached to our GYN reports reads: > >"The gynecological Pap test is a screening test designed to aid in the detection of cervical cancer and its precursors. Interpretation of the Pap test should not be used as the sole means of detecting cervical cancer." > > I hope this helps. > >Kevin D. Briggs, MS, CT(ASCP) >Team Leader- Cytopathology/ Histopathology Services >Danville Regional Medical Center >142 South Main Street >Danville, VA 24541 >Telephone: (434) 799-4470 ext.5451 >Fax: (434) 799-2118 >E-mail: briggsk@drhsi.org > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Victor >Tobias >Sent: Friday, March 25, 2005 11:44 AM >To: Rush, Joyce >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] GYN Report Disclaimer > > >We are JCAHO and CAP accreditted and have no disclaimer on our reports. >Is there something new that we need to be aware of? > >Victor > >Rush, Joyce wrote: > > > >>I know that most of you are Histology folks but hope that you will pass this on to any clinical Cytology co-workers. We are going from JCAHO to CAP for our surveys and need to add a report disclaimer to our GYN reports. I'd appreciate as many samples as I can get so I don't have to reinvent the wheel. >> >>Thanks so much! >> >>Joyce >> >>Joyce A. Rush, BS, MT(ASCP) >>Laboratory Manager >>St Joseph's Medical Center >>523 North Third Street >>Brainerd, MN 56401 >>Office:218-828-7500 Fax: 218-828-7510 >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From dellav <@t> musc.edu Fri Mar 25 14:31:08 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] GYN Report Disclaimer Message-ID: here is ours. Pap smear is a test that helps in early detection of some cancers of female organs. This test has a low, but not negligible, rate of error. In average women, there is a 1 in 1000 chance that abnormal cells will be missed by this test. There is a higher chance of missing abnormal cells in patients with some types of cancers or pre-cancerous conditions. Pap smear offers no guarantee for the detection of all cancers. This test must be performed annually for optimal detection. In high-risk patients, Pap smear testing must be performed more frequently Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Rush, Joyce" 03/25/05 11:36AM >>> I know that most of you are Histology folks but hope that you will pass this on to any clinical Cytology co-workers. We are going from JCAHO to CAP for our surveys and need to add a report disclaimer to our GYN reports. I'd appreciate as many samples as I can get so I don't have to reinvent the wheel. Thanks so much! Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Traczyk7 <@t> aol.com Fri Mar 25 17:20:27 2005 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Sliding vs. Sledge Microtome Message-ID: <100.1009573e.2f75f6bb@aol.com> Help me out here. What is the generally accepted distinction between a sliding and a sledge microtome? It seems to me that the terms get used interchangeably. In my book, a "sliding" microtome has a fixed specimen holder and the knife slides back and forth on a slide way. It is used for routine or frozen sectioning of samples. A "sledge" microtome has a fixed blade and the specimen is moved, either manually or motorized. The general application is for hard samples such as bone and in some material science applications. Any comments can be sent to me directly if you would like. Thanks, Dorothy Murphy Traczyk Hacker Instruments & Industries Inc. PO Box 1176 Winnsboro, SC 29180 From matt <@t> ategra.com Fri Mar 25 17:10:39 2005 From: matt <@t> ategra.com (Matt Hawes (extension 224)) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Histology Jobs Update PT and FT jobs Message-ID: Hello Histonetters, My name is Matt Hawes and I am the Senior AP Recruiter here at Ategra Systems and I have received New Histology Job Opportunities since I last mailed you. Here are some of my HOTTEST Fulltime & Parttime Permanent Histo Tech positions: 1. Illinois, Chicago- Histo Tech-Day Shift 2. Northern Michigan - Histo Day Shift 3. Los Angeles, CA- Imunohistochemistry Supervisor Day Shift 4. Long Island, NY-Part Time Histo Tech 20-30hrs wk 5. Boston Burbs, MA- MOHS Histo Tech Day Shift 6. Tampa, FL- Histo Tech all shifts 7. Las Vegas, NV- Histo Tech Days 8. Kansas City, MO- Histo Tech Day and Night Shift These positions are as direct empployees of our clients. They all offer full employee benefits: excellent healthcare, life insurance, dental, vision, 401K/retirement - as well as sign-on bonuses and relocation bonuses (where applic). If you are interested in these jobs, please CALL ME ASAP at (800)466 9919 x224 or e-mail me at Matt@ategra.com . To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well as I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who might be interested as well, if you could pass my query & name on to them I'd be very grateful. However, if you are interested in any of the jobs above, please call me. Thank You !! Matt Hawes Senior Laboratory Recruiter Ategra Systems (800) 466-9919 Ext 234 Matt@Ategra.com --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing VOICE: 407-671-5800 ext 224 TOLLFREE: 800-466-9919 Ext 224 Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu To Learn More About Ategra: http://www.ategra.com -------------------------------------------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. -------------------------------------------------------------------------------------------------- From Tom_Wells <@t> bcit.ca Fri Mar 25 23:10:17 2005 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Autopsy video Message-ID: Some of my students are doing a project on aut with this project I have tried to arrange for&nbs autopsy at a couple of our local hospitals, another it hasn't worked out. I was wondering if an any autopsy videos. The ones that I have seen are a littl hollywood. I want something that accurately reflects an autopsy. Any suggestions would be appreciated. Thanks. From teresajharris <@t> msn.com Sat Mar 26 13:48:54 2005 From: teresajharris <@t> msn.com (teresajharris@msn.com) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] copper stain for liver biopsies References: Message-ID: Hi Jim, I have performed this stain with good results. I purchased my stain kit and control slides from Poly Scientific. Phone number 800-645-5825. Just tell them what you want to stain and they will help you choose the right kit. Teresa Harris, HTL VA Medical Center ----- Original Message ----- From: Vickroy, Jim To: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 23, 2005 1:13 PM Subject: [Histonet] copper stain for liver biopsies We are looking for a procedure for staining for copper. In the past we have used an aldehyde fuchsin stain but our pathologist would like to use a rhodanine stain. Does anybody have a good procedure for staining copper and any suggestions where to get a kit or reagents? Jim Vickroy Memorial Medical Stain Springfield, Illinois _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Sun Mar 27 10:15:06 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] CK903 w/Feulgen Message-ID: FYI - CK903 should be referred to as "high molecular weight cytokeratin" or CK-34BetaE12 (the actual clone designation), not as CK903. The "903" came from the old Enzo catalog. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Carole Fields 03/16/05 08:05AM >>> A fellow Histo tech at the VA in Columbia, SC is looking for a vendor for CK903 w/Feulgen (34BE12 w/Feulgen). He is having a problem finding this particular antibody. Any help is appreciated. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 (803) 936-8214 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Mon Mar 28 10:40:50 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Automated Microtomes Message-ID: Renee, I have 2 Leica's 2255. I love them. I use them daily they have saved my wrist and shoulder. You can use them in automatic or manual mode. It is easy to go between manual and automatic. I have a foot pedal and I find it to be very efficient. I am strictly a research facility and work primarily on rodents infected with scrapie. We are working on numerous transgenic mice with deer/elk and other PrP genes. I wanted a microtome that I could use in a bio-safety hood so I could trap all the paraffin scraps when I am cutting. If you have ever cut mouse tissue you will appreciate that even in the best of circumstances it is very dry. I use the automatic mode on the tiniest of samples. Maybe not quite a tiny as biopsies but mouse lymph nodes are pretty small. Some of my work requires me to cut brain with precision so that we can sample multiple animals in a specific anatomic region. I have never had any difficulty doing this in the automatic mode. Can't say enough wonderful things about this microtome! Happy Shopping! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: rgrow@bmnet.com [mailto:rgrow@bmnet.com] Sent: Friday, March 25, 2005 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Microtomes Are there Histotechs who actually use automated microtomes daily? Not the ones you have purchased, then seldom use or use in a manual mode. I need real-time guidance from techs who use them daily and what likes/dislikes you have with the particular brand bought. Any response is appreciated. Thanks. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Mon Mar 28 10:42:28 2005 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Tech to Path Ratio Message-ID: <1DB04B57B04C5747B87C3B39F3E605AA02E450E9@harrier> Dear All, Just wondering, in a clinical/hospital service setting if there is a "rule of thumb" on tech to path ratio? Seems to me I was told it was 1:1.5 at one time, that being one pathologist supported by 1.5 technicians. I can't recall according to who/whom this ratio was determined. I have kept this ratio in mind for most of my career (basically working in a short staff mode even when fully staffed). It seems this question was previously addressed, not clearly to my recollection. I also wouldn't mind getting some opinions about this as we have of course gone through much change over the years. Automation and expansion of responsibilities factor into this equation, although in some respects it may be a wash as automation brings relief yet expansion creates more tasks. Thank you for responses and input. Thomas Jasper HT(ASCP)BAS Anatomic Pathology Coordinator SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From Tiffany.L.Sheffield <@t> uth.tmc.edu Mon Mar 28 11:12:11 2005 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Sheffield, Tiffany L) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Prostaglandin Ab Question Message-ID: <88137A668436CA4B87DCEFF4D695AA42CC4F5A@UTHEVS4.mail.uthouston.edu> Hello Fellow Histonetters! I was wondering if anyone could suggest a company that offers Ab's for Prostaglandin D2, E2, and F for IHC testing in paraffin embedded tissue. I have performed assays measuring the quantity in tissue but I was wondering if there are Ab's for IHC use? Thank you for your time. Tiffany Tiffany Sheffield-Lopez, HT(ASCP) Supervisor, Bone Histomorphometry & Biomaterials Lab Department of Orthopaedic Surgery UTHSC-Houston Medical School 6431 Fannin, Suite 6.144 MSB Houston , TX 77030 713-500-6803 WK 713-500-0729 Fax From petepath <@t> yahoo.com Mon Mar 28 11:46:10 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] re:Tech to Path Ratio Message-ID: <20050328174610.3356.qmail@web30403.mail.mud.yahoo.com> Hi Tom, A few years back when was struggling to get our administration to hire additional histotech I did an informal study of the staffing for histotechs in hospitals in the NY metropolitan area. I called about a dozen histology supervisors and simply asked how many histotechs they had and how many surgical specimens they processed. These were mostly large busy acadmic or affiliated hospitals. Excluding us, my results ranged from of one histotech per 2000 sugicals up to one pre 3500 surgicals. The average was one pre 2600 sugicals. We were at one per 4500. Putting this information in front my administrators, I told them the choice was to add personel or to start reducing the number of sections and special studies which would certainly lessen our quality of care. I was able to convince them add three histotechs and a supervisor. I am not sure how these simple ratios would apply to other types of hospitals but it was a pretty good indication that we were understaffed. With a little effort, a telephone and generous colleagues, I was able to compile some useful data. The moral of this story is that compiling such data can be a fairly easy task and you can present factual data which makes it much harder for your administration to wiggle away. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From cwscouten <@t> myneurolab.com Mon Mar 28 11:48:47 2005 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Sliding vs. Sledge Microtome Message-ID: <5784D843593D874C93E9BADCB87342AB44F7A9@tpiserver03.Coretech-holdings.com> Your definition conforms to what I have seen, that sliding means moving blade, and sledge means moving tissue. However, I do not think there is any functional difference. They can be used for all the same applications, are both grouped under the category "sliding microtome". Does anybody in histoland who has used both feel they have different applications? There is a clear safety difference, but the tissue doesn't care which moves. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Traczyk7@aol.com Sent: Friday, March 25, 2005 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sliding vs. Sledge Microtome Help me out here. What is the generally accepted distinction between a sliding and a sledge microtome? It seems to me that the terms get used interchangeably. In my book, a "sliding" microtome has a fixed specimen holder and the knife slides back and forth on a slide way. It is used for routine or frozen sectioning of samples. A "sledge" microtome has a fixed blade and the specimen is moved, either manually or motorized. The general application is for hard samples such as bone and in some material science applications. Any comments can be sent to me directly if you would like. Thanks, Dorothy Murphy Traczyk Hacker Instruments & Industries Inc. PO Box 1176 Winnsboro, SC 29180 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Mon Mar 28 11:50:48 2005 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Flammable Regulations for Formalin Message-ID: Hi Histonetters, Formalin has a flammable rating of 1. EPA says that more than 10 gallons of flammable and combustible liquids should be stored in a flammables cabinet or specially designed room. Storage in flammable cabinets must not exceed 60 gallons. How is everyone storing their saved tissues? We currently store ours in a vented cabinet(not a flammable cabinet). Do flammables with a rating of 1 have to be stored the same as flammables with ratings of 3? Nancy Levin Safety Officer Luther Pathology Lab Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From jkiernan <@t> uwo.ca Mon Mar 28 12:01:52 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Prostaglandin Ab Question References: <88137A668436CA4B87DCEFF4D695AA42CC4F5A@UTHEVS4.mail.uthouston.edu> Message-ID: <42484690.68DE6009@uwo.ca> Prostaglandins are lipids. Is there any chance of them surviving in paraffin sections? John Kiernan. --------------------------------------- "Sheffield, Tiffany L" wrote: > > Hello Fellow Histonetters! > > I was wondering if anyone could suggest a company that offers Ab's > for Prostaglandin D2, E2, and F for IHC testing in paraffin embedded > tissue. I have performed assays measuring the quantity in tissue but I > was wondering if there are Ab's for IHC use? Thank you for your time. > Tiffany > > Tiffany Sheffield-Lopez, HT(ASCP) > Supervisor, Bone Histomorphometry & Biomaterials Lab > Department of Orthopaedic Surgery > UTHSC-Houston Medical School > 6431 Fannin, Suite 6.144 MSB > Houston , TX 77030 > 713-500-6803 WK > 713-500-0729 Fax > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Mon Mar 28 12:18:51 2005 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Prostaglandin Ab Question Message-ID: Try Novus Biologicals or Abcam. Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheffield, Tiffany L Sent: Monday, March 28, 2005 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostaglandin Ab Question Hello Fellow Histonetters! I was wondering if anyone could suggest a company that offers Ab's for Prostaglandin D2, E2, and F for IHC testing in paraffin embedded tissue. I have performed assays measuring the quantity in tissue but I was wondering if there are Ab's for IHC use? Thank you for your time. Tiffany Tiffany Sheffield-Lopez, HT(ASCP) Supervisor, Bone Histomorphometry & Biomaterials Lab Department of Orthopaedic Surgery UTHSC-Houston Medical School 6431 Fannin, Suite 6.144 MSB Houston , TX 77030 713-500-6803 WK 713-500-0729 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From gliuygao <@t> hotmail.com Mon Mar 28 12:24:28 2005 From: gliuygao <@t> hotmail.com (yan gao) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] ERK antibody Message-ID: Dear Histonet, Does anyone done phospho-ERK antibody? I am working on the RAF pathway need to stain pERK IHC in human tumor paraffin section. Can anyone give some tips? THank a lot. Yan Gao Novartis From Tiffany.L.Sheffield <@t> uth.tmc.edu Mon Mar 28 12:39:28 2005 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Sheffield, Tiffany L) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Prostaglandin Ab Question Message-ID: <88137A668436CA4B87DCEFF4D695AA42CC4F79@UTHEVS4.mail.uthouston.edu> I'm sure the probably is very minuscule. I just thought I would try but you are right. -----Original Message----- From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Monday, March 28, 2005 12:02 PM To: Sheffield, Tiffany L Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Prostaglandin Ab Question Prostaglandins are lipids. Is there any chance of them surviving in paraffin sections? John Kiernan. --------------------------------------- "Sheffield, Tiffany L" wrote: > > Hello Fellow Histonetters! > > I was wondering if anyone could suggest a company that offers > Ab's for Prostaglandin D2, E2, and F for IHC testing in paraffin > embedded tissue. I have performed assays measuring the quantity in > tissue but I was wondering if there are Ab's for IHC use? Thank you for your time. > Tiffany > > Tiffany Sheffield-Lopez, HT(ASCP) > Supervisor, Bone Histomorphometry & Biomaterials Lab Department of > Orthopaedic Surgery UTHSC-Houston Medical School > 6431 Fannin, Suite 6.144 MSB > Houston , TX 77030 > 713-500-6803 WK > 713-500-0729 Fax > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Mar 28 13:53:04 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Sliding vs. Sledge Microtome In-Reply-To: <5784D843593D874C93E9BADCB87342AB44F7A9@tpiserver03.Coretec h-holdings.com> References: <5784D843593D874C93E9BADCB87342AB44F7A9@tpiserver03.Coretech-holdings.com> Message-ID: <6.0.0.22.1.20050328113700.01b36bc0@gemini.msu.montana.edu> Took a look at Leica's sliding microtomes, they use the terms sliding and sledge interchangeably with these LARGE sliding microtomes. One can use these for large whole specimen sectioning i.e. prostates, eyes, or bone work, and in case of the SM2400 - wood products industry. It seems the sledge (aka sled) can either hold a blade and go sliding to a block OR the sledge can hold a block to go sliding to the blade. The manually operated model Leitz 1500 now known as an Leica SM2400 sliding microtome. On the latter they talk about a sledge locking device on the "sled" (definition of sledge!) part of the microtome where the specimen is clamped. The Leica SM2500 (aka Polycut) sliding microtome, in conjunction with its use with an an ultramiller, also referred to as a sledge microtome in their description of this instrument. Once again it is the sample (commonly a methylmethacrylate embedded calcified bone block clamped onto a sledge for sectioning by a stationary tungsten carbide blade OR a milled by a stationary ultramiller.). I have seen Polycuts inside cryochambers and there are even bigger, pricier cryostats available - one we considered for whole mouse cryosectioning but the $200,000 plus price tag and huge size were limiting factors. Leica has a third sliding microtome with blade moving to the sample, or at least that is what I could deduce from reading their description of instrument operation. Go to LeicaMicrosystem website, specifically http://www.histo-solutions.com/website/sc_hbu.nsf and look at their sliding/sledge microtomes. We never felt any one of our sliding microtomes was any safer than the other. They required great concentration to operate preferrably behind closed doors and no sudden startle factors/distractions. Both our sliding microtomes were superbly capable of finger amputation. At 10:48 AM 3/28/2005, you wrote: >Your definition conforms to what I have seen, that sliding means moving >blade, and sledge means moving tissue. However, I do not think there is >any functional difference. They can be used for all the same >applications, are both grouped under the category "sliding >microtome". Does anybody in histoland who has used both feel they have >different applications? There is a clear safety difference, but the >tissue doesn't care which moves. > > >Cordially, >Charles W. Scouten, Ph.D. >myNeuroLab.com >5918 Evergreen Blvd. >St. Louis, MO 63134 >Ph: 314 522 0300 >FAX 314 522 0377 >cwscouten@myneurolab.com >http://www.myneurolab.com > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Traczyk7@aol.com >Sent: Friday, March 25, 2005 5:20 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Sliding vs. Sledge Microtome > >Help me out here. >What is the generally accepted distinction between a sliding and a sledge >microtome? It seems to me that the terms get used interchangeably. In my >book, a "sliding" microtome has a fixed specimen holder and the >knife slides back and forth on a slide way. It is used for routine or >frozen sectioning of samples. A "sledge" microtome has a fixed blade and >the specimen is moved, either manually or motorized. The general >application is for hard samples such as bone and in some material >science applications. >Any comments can be sent to me directly if you would like. >Thanks, >Dorothy Murphy Traczyk >Hacker Instruments & Industries Inc. From Sue.Kapoor <@t> uhsi.org Mon Mar 28 14:49:45 2005 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Need used hood for Tissue-Tek DRS-601 stainer Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0B0@khmcexch.uhsi.org> To all Vendors - I'm looking for a used/refurbished hood for this stainer. Please email off line with quotes to: Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 Thank you! From NSEARCY <@t> swmail.sw.org Mon Mar 28 15:20:28 2005 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Tissue Cassettes Message-ID: Are there any new "biopsy" cassettes o the market? Know of multi-chambered with tiny holes- as well as "biopsy" cassettes. Have had some "lost" "small" specimens lately and chief pathologist over histology does not want to use papers or bags. Any help would be appreciated. Nita From Tiffany.L.Sheffield <@t> uth.tmc.edu Mon Mar 28 15:28:10 2005 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Sheffield, Tiffany L) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Prostaglandin Ab Question Message-ID: <88137A668436CA4B87DCEFF4D695AA42CC4FCC@UTHEVS4.mail.uthouston.edu> Thanks to all who responded to my questions. You gave me some great leads and possible solutions. The HistoNet rocks! -----Original Message----- From: Connolly, Brett M [mailto:brett_connolly@merck.com] Sent: Monday, March 28, 2005 12:19 PM To: Sheffield, Tiffany L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Prostaglandin Ab Question Try Novus Biologicals or Abcam. Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheffield, Tiffany L Sent: Monday, March 28, 2005 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostaglandin Ab Question Hello Fellow Histonetters! I was wondering if anyone could suggest a company that offers Ab's for Prostaglandin D2, E2, and F for IHC testing in paraffin embedded tissue. I have performed assays measuring the quantity in tissue but I was wondering if there are Ab's for IHC use? Thank you for your time. Tiffany Tiffany Sheffield-Lopez, HT(ASCP) Supervisor, Bone Histomorphometry & Biomaterials Lab Department of Orthopaedic Surgery UTHSC-Houston Medical School 6431 Fannin, Suite 6.144 MSB Houston , TX 77030 713-500-6803 WK 713-500-0729 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------ ------ From JWEEMS <@t> sjha.org Mon Mar 28 15:32:41 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Tissue Cassettes Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA4576F@sjhaexc02.sjha.org> Surgipath has a really good one. 800-225-3035. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nita Searcy Sent: Monday, March 28, 2005 4:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Cassettes Are there any new "biopsy" cassettes o the market? Know of multi-chambered with tiny holes- as well as "biopsy" cassettes. Have had some "lost" "small" specimens lately and chief pathologist over histology does not want to use papers or bags. Any help would be appreciated. Nita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From karenharmon <@t> comcast.net Mon Mar 28 16:25:33 2005 From: karenharmon <@t> comcast.net (Karen Harmon) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Pneumocystis carnii staining Message-ID: <000601c533e5$0f9c0e20$12dca643@CPQ20882111713> We use a GMS stain to demonstrate pneuomcyctis with successful results about 50% of the time. We have taken this procedure apart step by step, solution by solution and are at our wits end to determine the cause for failure. I can assure you that over the past 3 weeks, there is no variable that we have not tried. The ironic part is we never have a problem demonstrating fungi with the GMS, in fact, we never had any problems at all with other silver procedures. Can anyone help with our hit and miss problem with pneumocystis? Karen Harmon HT (ASCP) From jqb7 <@t> cdc.gov Mon Mar 28 16:36:26 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Pneumocystis carnii staining Message-ID: Does your Pneumoscystis carinii control stain properly or is it the diagnostic tissue and the control? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Karen Harmon Sent: Mon 3/28/2005 5:25 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Pneumocystis carnii staining We use a GMS stain to demonstrate pneuomcyctis with successful results about 50% of the time. We have taken this procedure apart step by step, solution by solution and are at our wits end to determine the cause for failure. I can assure you that over the past 3 weeks, there is no variable that we have not tried. The ironic part is we never have a problem demonstrating fungi with the GMS, in fact, we never had any problems at all with other silver procedures. Can anyone help with our hit and miss problem with pneumocystis? Karen Harmon HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Mar 28 16:41:37 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] BiopsyTissue Cassette In-Reply-To: References: Message-ID: <6.0.0.22.1.20050328152643.01b30498@gemini.msu.montana.edu> Nita, We have gone to the All in One Cassette, from Histoplex. A small triangular area on one corner of cassette has tiny holes and a tight fitting lid in that area for biopsies. For all other tissues, you use the large area with standard size slots. They come in 11 colors. They are working very well for us - no longer have to keep two kinds of cassettes around!! Very efficient and saves space and the techs grossing in research tissues LOVE them!!! On embedding and microtomy end, we like them too. Available from Fisher white are Cat # 221464420. Our samples came from American MasterTech Scientific, ph # 800 860-4073 go to www.mastertechs.com to take a look at these. They will send samples so you can try before investing. Their prices are competitive. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From llewllew <@t> shaw.ca Mon Mar 28 16:44:10 2005 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Sliding vs. Sledge Microtome References: <5784D843593D874C93E9BADCB87342AB44F7A9@tpiserver03.Coretech-holdings.com> Message-ID: <000a01c533e7$a8bb8250$7e034246@yourlk4rlmsu> I learned to section on an MSE base sledge microtome many years ago, as did most British technologists of the time. Not only is the knife fixed it is also clamped in place. The tissue is also clamped onto a stage which could move back/forward by pushing, and up/down on a screw thread keyed to the back/forward movement or by releasing a spring closed clamping nut. The sliding microtomes which I have used had fixed, clamped tissue on a fixed stage keyed to the knife movement and also up/down by a screw thread. The significant difference is in the knife. It was clamped into a holder with an offset V-shaped bottom which rested in a complementary shaped groove by weight alone with no clamping. If a hard tissue was struck (calcified tissue), the knife holder would jump since it was not clamped to the main body, but held in place by gravity and perhaps a small lip on top of the groove. The microtomes were operated differently. The sledge usually was used with a wedge shaped knife with a fairly short distance between edge and back so that it was very rigid. A small slant was used on the knife, but mostly a small area was used by pushing a paraffin block across the edge and peeling the section off. One of the hazards was pushing the block too far too fast and nicking the fingers and nuckles, as the scars on my right hand testify. The sliding microtome usually used an extra plano-concave knife which was fairly wide and very thin along the cutting edge. It vibrated very easily and could not take too much force applied to it before it chattered. It was not usually used with paraffin blocks because they chattered too easily. It was more commonly used for celloidin sectioning at 15-20 microns. The knife was slanted enough so that the whole edge was used to cut each section, and it was sliced off the block instead of peeled. It took a lot of practice to use this microtome effectively, and I never did enough of it to become really adept. Thanks for the trip down memory lane. Bryan Llewellyn ----- Original Message ----- From: "Charles Scouten" To: ; Sent: Monday, March 28, 2005 9:48 AM Subject: RE: [Histonet] Sliding vs. Sledge Microtome Your definition conforms to what I have seen, that sliding means moving blade, and sledge means moving tissue. However, I do not think there is any functional difference. They can be used for all the same applications, are both grouped under the category "sliding microtome". Does anybody in histoland who has used both feel they have different applications? There is a clear safety difference, but the tissue doesn't care which moves. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Traczyk7@aol.com Sent: Friday, March 25, 2005 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sliding vs. Sledge Microtome Help me out here. What is the generally accepted distinction between a sliding and a sledge microtome? It seems to me that the terms get used interchangeably. In my book, a "sliding" microtome has a fixed specimen holder and the knife slides back and forth on a slide way. It is used for routine or frozen sectioning of samples. A "sledge" microtome has a fixed blade and the specimen is moved, either manually or motorized. The general application is for hard samples such as bone and in some material science applications. Any comments can be sent to me directly if you would like. Thanks, Dorothy Murphy Traczyk Hacker Instruments & Industries Inc. PO Box 1176 Winnsboro, SC 29180 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.reilly <@t> jcu.edu.au Mon Mar 28 18:35:10 2005 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Natural Dyes: Beet Juice In-Reply-To: <424303B4.9070609@umdnj.edu> References: Message-ID: <5.1.0.14.0.20050329102833.00a4e1d0@mail.jcu.edu.au> Dev and Histonetters, Mulberry juice works well as a nuclear stain, if you add a mordant such as aluminium ammonium sulphate. Haematoxylin is a natural dye. If you take some sawdust from the heartwood of Heamatoxylum campechianum, mix it with water to give a blood red solution,filter, then add a mordant you will have a solution that will stain nucleii. Regards, Laurie. At 10:15 AM 03/24/05 -0800, Geoff McAuliffe wrote: >Hi Anurag: > > Get yourself a copy of "Natural dyes and home dyeing" by Rita Adrosko. > It is published by Dover and is not expensive. Lots of recipes and a > pretty good bibliography. > >Geoff > >Anurag Sharma wrote: > >>Hi, >> >>I am a first year Med Lab Science student and am doing a project on >>"Natural Dyes". Has anyone here ever tried using Beet juice to stain >>tissues? If yes, could you please tell me something about it (like the >>extraction procedure, mordant used, pH and what does it stain). Is there >>any other Natural dye you would suggest me to use? >> >>Dev >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > >-- >-- >********************************************** >Geoff McAuliffe, Ph.D. >Neuroscience and Cell Biology >Robert Wood Johnson Medical School >675 Hoes Lane, Piscataway, NJ 08854 >voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu >********************************************** > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly Ph 07 4781 4468 School of Veterinary & Biomedical Science Fax 07 4779 1526 James Cook University Townsville Qld. 4811 laurie.reilly@jcu.edu.au Australia. From lpwenk <@t> sbcglobal.net Mon Mar 28 19:26:38 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Flammable Regulations for Formalin References: Message-ID: <003a01c533fe$5e294580$e5cdd545@domainnotset.invalid> Formaldehyde, which is 37-40% formaldehyde, has a NFPA flammability rating of 2. It has a flash point somewhere between 50 to 60 degrees C. (122 to 140 degrees C.) (different MSDS have different numbers). It is the formaldehyde gas vapors which, when mixed with air, that are flammable. Formalin, which is usually 10% formalin and thus only 3.7-4.0% formaldehyde, is 1/10 the concentration of the above formaldehyde, or 90% more water. It has a flammability rating of 0 to 1, again depending upon which company's MSDS you are reading. It has a flash point of 85-90 degrees C (185 to greater than 195 degrees F). Being more dilute, the formaldehyde in the 10% formalin is more difficult to ignite. By definition, flammable means the chemical has a flashpoint below 100 degrees F. Combustible means it has a flashpoint between 100 and 200 degrees F. So both formalin and formaldehyde gases, when mixed with air, are combustible, but not flammable. I'm not at work, so I don't have the regs in front of me, but maybe some kind Histonetter can find them. I believe the provision is that formaldehyde/formalin can be stored in a cool, well ventilated area away from all sources of heat, sparks and flames, and away from incompatible chemicals (such as oxidizers). That the regs don't specify flammable cabinets. Let me know if my memory and interpretation are correct. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Bauer, Karen" To: Sent: Monday, March 28, 2005 12:50 PM Subject: [Histonet] Flammable Regulations for Formalin Hi Histonetters, Formalin has a flammable rating of 1. EPA says that more than 10 gallons of flammable and combustible liquids should be stored in a flammables cabinet or specially designed room. Storage in flammable cabinets must not exceed 60 gallons. How is everyone storing their saved tissues? We currently store ours in a vented cabinet(not a flammable cabinet). Do flammables with a rating of 1 have to be stored the same as flammables with ratings of 3? Nancy Levin Safety Officer Luther Pathology Lab Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From IKirbis <@t> onko-i.si Tue Mar 29 02:47:02 2005 From: IKirbis <@t> onko-i.si (=?iso-8859-2?Q?Kirbi=B9_Srebotnik_Irena?=) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] LIS in cytology labs Message-ID: Dear netters, I wonder how do other cyto/patho labs use the LIS in their workflow, especially at the sample arrival and accessioning, how do you generate sample numbers, do you have to enter patient data first to get new number, do you have PC on the dirty desk where the samples are receiving, do you have two technicians to access the samples, one for the data entry and one for the sample accessioning? your responses would be very appreciated Irena Srebotnik Kirbi?, MSc Institute of Oncology Dept. of Cytopathology Zalo?ka 2 1000 Ljubljana Slovenia phone +386 1 522 3826 fax +38615879400 From gu.lang <@t> gmx.at Tue Mar 29 05:41:30 2005 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:24:50 2005 Subject: AW: [Histonet] Sliding vs. Sledge Microtome In-Reply-To: <5784D843593D874C93E9BADCB87342AB44F7A9@tpiserver03.Coretech-holdings.com> Message-ID: We use sliding microtomes (Fa. Microm) for our routine histology, like most of you probably use the rotary microtomes. The bigger sledge microtomes, I consider, are mostly used with resinembeddings of hard material. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Charles Scouten Gesendet: Montag, 28. M?rz 2005 19:49 An: Traczyk7@aol.com; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Sliding vs. Sledge Microtome Your definition conforms to what I have seen, that sliding means moving blade, and sledge means moving tissue. However, I do not think there is any functional difference. They can be used for all the same applications, are both grouped under the category "sliding microtome". Does anybody in histoland who has used both feel they have different applications? There is a clear safety difference, but the tissue doesn't care which moves. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Traczyk7@aol.com Sent: Friday, March 25, 2005 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sliding vs. Sledge Microtome Help me out here. What is the generally accepted distinction between a sliding and a sledge microtome? It seems to me that the terms get used interchangeably. In my book, a "sliding" microtome has a fixed specimen holder and the knife slides back and forth on a slide way. It is used for routine or frozen sectioning of samples. A "sledge" microtome has a fixed blade and the specimen is moved, either manually or motorized. The general application is for hard samples such as bone and in some material science applications. Any comments can be sent to me directly if you would like. Thanks, Dorothy Murphy Traczyk Hacker Instruments & Industries Inc. PO Box 1176 Winnsboro, SC 29180 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Mar 29 07:18:43 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] LIS in cytology labs Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA4577F@sjhaexc02.sjha.org> We have a specimen accessioning area in both Histology and Cytology. Specimens are accessioned as they are received - into LIS with patient info. The patients or outpatient specimens are first registered into the hospital IS. A medical record number, name or social security number pulls the patient info into the LIS from the hospital system. The requisitions and specimen containers are then labeled with a bar code label and moved the grossing/ThinPrep processing area. Hope this helps! j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kirbi? Srebotnik Irena Sent: Tuesday, March 29, 2005 3:47 AM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] LIS in cytology labs Dear netters, I wonder how do other cyto/patho labs use the LIS in their workflow, especially at the sample arrival and accessioning, how do you generate sample numbers, do you have to enter patient data first to get new number, do you have PC on the dirty desk where the samples are receiving, do you have two technicians to access the samples, one for the data entry and one for the sample accessioning? your responses would be very appreciated Irena Srebotnik Kirbi?, MSc Institute of Oncology Dept. of Cytopathology Zalo?ka 2 1000 Ljubljana Slovenia phone +386 1 522 3826 fax +38615879400 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From KDwyer3322 <@t> aol.com Tue Mar 29 07:58:13 2005 From: KDwyer3322 <@t> aol.com (KDwyer3322@aol.com) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Texas Society For Histotechnology 2005 Symposium/Convention Message-ID: <1e3.387a1082.2f7ab8f5@aol.com> It's not to late to join the Texas Society for Histotechnology 2005 Symposium/Convention attached is the program or go to www.txsh.org "Not Just a Plano Meeting - Histology 2005" Texas Society for Histotechnology 2005 Symposium/Convention Marriott Dallas/Plano at Legacy Town Center 7120 Dallas Parkway, Plano, Texas Thursday, April 21, 2005 - 11:00 a.m. - Golf Outing Tribute Golf Course Friday, April 22, 2005 2:00-5:30 p.m. Workshop 1) HT (ASCP) Examination Readiness - Glenda Hoye Saturday April 23, 2005 8-11:30 A.M. Morning Workshops 2) Quality Issues in Immunohistochemistry - Bryan Hewlett 3) Microwave: The Whole Enchilada - Donna Willis and Jan Minshew 4) Advanced Excellence with Routine H&E Staining and Immunochemical Staining Of Bone Specimens and Bone Marrow Biopsies Following Decalcifications - James Biesecker, M.D. PhD 5) Do You Know What Your Laboratory Workflow Is? - Ms. Ritu Ward Morning Symposiums 1) Immunohistochemistry in the Analysis of Forensic Evidence from a Double Homicide in New Zealand: The Lundy Case - Rodney Miller, M.D. 2) Antibodies As Diagnostic Tools And Their Utility Beyond Diagnostics: Discussion using GI Stromal tumors/KIT as an example - Saime Aksoy, M.D. 3) Keys to Understanding and Applying Academic Articles in the Histopathology Laboratory - Mark Bailey Saturday April 23, 2005 1:00-4:30p.m. Afternoon Workshops 6) Technical Immunohistochemistry: Achieving Reliability and Reproducibility of Immunostains - Rodney Miller, M.D. 7) Is Your Tissue Processor Fighting You? Fight Back - Pam Marcum 8) Preparing for a CAP Inspection - Hector Hernandez and Joe Nocito 9) Color Your World - an Overview of Special Stains - Kim Rhatigan-Drexler Afternoon Symposiums Student Presentations- Texas Histology Schools 4a) - Cancer: A Collection of Diseases - Olukayode Awotesu, Sujatha Kakuru, and Umadevi Narra - MD Anderson Cancer Center and Mary Anderson - Houston Community College 4b) - Special Histologic Techniques - Michael Scabeto - St. Phillips College and Yvonne Koening University of Texas Health Science Center in San Antonio Saturday, April 23, 2005 - 6:30-7:30 p.m. Guest Speaker - Beck Weathers, M.D. Sunday April 24, 2005 8-11:30 A.M. Workshops 10) IHC Mathematics in the Laboratory - Joel Martinez 11) Shipping Diagnostic Specimens- Linda Durbin 12) Quality Standards for Staining - Bryan Hewlett 13) Quality Assurance in the Histology Laboratory - Kathy Dwyer and Debbie Siena Symposiums 5) Team Building 101 - Evelyn Sandberg 6) Pathologists Looks at the Passion & Death of Jesus Christ- Kevin J. McQuaid, M.D. Program Information - Veronica Davis - 972-579-8291 Vendor Information - Judy Webb - 817-927-1024 Golf Outing Information - Donna Willis -817-878-5644 From Kemlo.Rogerson <@t> elht.nhs.uk Tue Mar 29 08:15:58 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Sliding vs. Sledge Microtome[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0F4@bhrv-nt-11.bhrv.nwest.nhs.uk> Oddly, in the UK, and I may be wrong on this, but...... There is a North/ South divide. From my experience the South tends to use sledges (sliding microtomes) whilst the North use rotaries (except for the hard stuff that needs a sledge). From my experience there is a tendency for ribbon sections to be 'thick' and 'thin' (alternate ones that is) when cut on a sledge and it has been suggested that rotaries can lead to 'chatters'. >From my own experience I would not choose a sledge for biopsies, especially if ribbons or levels are to be cut. Why there is a 'North'/ South' divide eludes, maybe we Northerners eat more bacon. Have you ever seen bacon cut on a sledge!!!!! -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: 29 March 2005 12:42 To: Histonetliste (Histonetliste) Subject: AW: [Histonet] Sliding vs. Sledge Microtome[Scanned] We use sliding microtomes (Fa. Microm) for our routine histology, like most of you probably use the rotary microtomes. The bigger sledge microtomes, I consider, are mostly used with resinembeddings of hard material. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Charles Scouten Gesendet: Montag, 28. M?rz 2005 19:49 An: Traczyk7@aol.com; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Sliding vs. Sledge Microtome Your definition conforms to what I have seen, that sliding means moving blade, and sledge means moving tissue. However, I do not think there is any functional difference. They can be used for all the same applications, are both grouped under the category "sliding microtome". Does anybody in histoland who has used both feel they have different applications? There is a clear safety difference, but the tissue doesn't care which moves. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Traczyk7@aol.com Sent: Friday, March 25, 2005 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sliding vs. Sledge Microtome Help me out here. What is the generally accepted distinction between a sliding and a sledge microtome? It seems to me that the terms get used interchangeably. In my book, a "sliding" microtome has a fixed specimen holder and the knife slides back and forth on a slide way. It is used for routine or frozen sectioning of samples. A "sledge" microtome has a fixed blade and the specimen is moved, either manually or motorized. The general application is for hard samples such as bone and in some material science applications. Any comments can be sent to me directly if you would like. Thanks, Dorothy Murphy Traczyk Hacker Instruments & Industries Inc. PO Box 1176 Winnsboro, SC 29180 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Mar 29 08:28:35 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Tissue Cassettes Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41F2@fh2k093.fhmis.net> Nita, We use "mesh" cassettes from Allegiance with good results - mostly for brain bx's. There is a fine "screen mesh" instead of slitted openings. Janet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: 3/28/2005 4:20 PM Subject: [Histonet] Tissue Cassettes Are there any new "biopsy" cassettes o the market? Know of multi-chambered with tiny holes- as well as "biopsy" cassettes. Have had some "lost" "small" specimens lately and chief pathologist over histology does not want to use papers or bags. Any help would be appreciated. Nita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Mar 29 08:33:39 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Pneumocystis carnii staining Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41F3@fh2k093.fhmis.net> We had this problem until we stopped using Periodic Acid instead of Chromic Acid. Use the 4% Chromic Acid at 60 degrees C for an hour (we use a deep waterbath) -or with a microwave and let sit for 20 minutes. We also had a problem with our control not being good enough (too old, I guess). We now purchase our controls from 'Newcomer' and they are excellent. Janet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: 3/28/2005 5:25 PM Subject: [Histonet] Pneumocystis carnii staining We use a GMS stain to demonstrate pneuomcyctis with successful results about 50% of the time. We have taken this procedure apart step by step, solution by solution and are at our wits end to determine the cause for failure. I can assure you that over the past 3 weeks, there is no variable that we have not tried. The ironic part is we never have a problem demonstrating fungi with the GMS, in fact, we never had any problems at all with other silver procedures. Can anyone help with our hit and miss problem with pneumocystis? Karen Harmon HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abright <@t> brightinstruments.com Tue Mar 29 08:59:26 2005 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Sliding vs. Sledge Microtome Message-ID: A sliding microtome has the knife moving over the specimen to section, the sledge microtome has the specimen moving under the knife to section. Not much difference either way you would think, but there is, as with a sliding microtome the knife is only held on one end, on a sledge microtome the knife is mainly clamped on both ends, but can also be held on one end too. Therefore the sledge is more conventional in its cutting action and its knife clamping arrangement, and much more robust for a wider range of specimens hard and soft. My last point is that I do not think sliding microtomes have retraction. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Traczyk7@aol.com [mailto:Traczyk7@aol.com] Sent: 25 March 2005 23:20 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sliding vs. Sledge Microtome Help me out here. What is the generally accepted distinction between a sliding and a sledge microtome? It seems to me that the terms get used interchangeably. In my book, a "sliding" microtome has a fixed specimen holder and the knife slides back and forth on a slide way. It is used for routine or frozen sectioning of samples. A "sledge" microtome has a fixed blade and the specimen is moved, either manually or motorized. The general application is for hard samples such as bone and in some material science applications. Any comments can be sent to me directly if you would like. Thanks, Dorothy Murphy Traczyk Hacker Instruments & Industries Inc. PO Box 1176 Winnsboro, SC 29180 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David_S._Ahmed/HOU/UTMDACC <@t> mdanderson.org Tue Mar 29 09:14:26 2005 From: David_S._Ahmed/HOU/UTMDACC <@t> mdanderson.org (David_S._Ahmed/HOU/UTMDACC@mdanderson.org) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Metal Embedding Molds Message-ID: For those labs using the metal embedding molds, how often do you clean them with xylene and alcohol? David Ahmed From juan.gutierrez <@t> christushealth.org Tue Mar 29 09:29:55 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Metal Embedding Molds Message-ID: We boil them in soapy water every day. After boiling let them cool and the paraffin and junk floats to the surface and solidifies. Remove the paraffin and rinse in running water. Dry in the oven. After the dry step you can spray mold release agents or use them without. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David_S._Ahmed/HOU/UTMDACC@mdanderson.org Sent: Tuesday, March 29, 2005 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal Embedding Molds For those labs using the metal embedding molds, how often do you clean them with xylene and alcohol? David Ahmed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Tue Mar 29 09:39:49 2005 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Metal Embedding Molds Message-ID: I run them through the purge cycle on the processor about once a week. I prefer not to use mold release. It seems to affect the ribboning quality of the blocks. Fred >>> 03/29/05 10:14AM >>> For those labs using the metal embedding molds, how often do you clean them with xylene and alcohol? David Ahmed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PatPatterson <@t> mhd.com Tue Mar 29 09:50:50 2005 From: PatPatterson <@t> mhd.com (Patterson, Pat) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Biopsy Cassettes Message-ID: <293C7C19EFF7D611AE1A0002A53F81140FA63497@omega.mhd.com> There was talk today about the different biopsy cassettes that are on the market. Have any of the users had any problems with processing problems related to them -- such as having trapped reagent begin carried over to the next processing step? Using 2 different types biopsy cassettes we've had several cases where several biopsies out of 50 appear not to be infiltrated - looking like those unfixed breast cases where they over-stuff the cassettes. Thanks Pat Patterson *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. From David.Edmondson <@t> christie-tr.nwest.nhs.uk Tue Mar 29 10:11:56 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Pb staining Message-ID: Hello, I mailed a collegue at the University who has lots of experience with metals, one John Denton. He replies thus "Staining for lead in tissues is somewhat old hat as the technique has been superseded by electron microprobe , laser ablation plasma coupled mass spectrometry and other beam techniques that allow ultra localisation with spatial distribution. We use both of these techniques for other metals but I have to beg the instrument time from others. It is many, many years since I stained for lead and have forgot anything useful. " Which may or may not be helpful Best wishes David Edmondson Christie Hosp Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Frank, Matthew Sent: 24 March 2005 16:11 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Pb staining Chuck Churukian has asked me to post the following for him. Does anyone out there have experience with Lead staining? Currently he is working with the sodium rhodizonate method. Are there other methods that anyone would recommend? Also, control blocks seem to be hard to come by, any sources or suggestions. Thank you all in advance for your input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Tue Mar 29 10:26:45 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Target retrieval solution Message-ID: <20050329162645.87101.qmail@web52602.mail.yahoo.com> Dako makes a 10x target retrieval solution that a lot of people use in the decloaker but I've heard so many different times being used; ie; 20 min, 10 min, 2 min. or does it matter? suggestions. Larry W. --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From jqb7 <@t> cdc.gov Tue Mar 29 10:47:24 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Pneumocystis carnii staining Message-ID: We always use chromic for our GMS. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Tuesday, March 29, 2005 9:34 AM To: 'Karen Harmon '; 'histonet-bounces@lists.utsouthwestern.edu '; 'histonet@lists.utsouthwestern.edu ' Subject: RE: [Histonet] Pneumocystis carnii staining We had this problem until we stopped using Periodic Acid instead of Chromic Acid. Use the 4% Chromic Acid at 60 degrees C for an hour (we use a deep waterbath) -or with a microwave and let sit for 20 minutes. We also had a problem with our control not being good enough (too old, I guess). We now purchase our controls from 'Newcomer' and they are excellent. Janet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: 3/28/2005 5:25 PM Subject: [Histonet] Pneumocystis carnii staining We use a GMS stain to demonstrate pneuomcyctis with successful results about 50% of the time. We have taken this procedure apart step by step, solution by solution and are at our wits end to determine the cause for failure. I can assure you that over the past 3 weeks, there is no variable that we have not tried. The ironic part is we never have a problem demonstrating fungi with the GMS, in fact, we never had any problems at all with other silver procedures. Can anyone help with our hit and miss problem with pneumocystis? Karen Harmon HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Thweatt <@t> med.va.gov Tue Mar 29 10:52:24 2005 From: John.Thweatt <@t> med.va.gov (John.Thweatt@med.va.gov) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Cutting Skin Lesions with keratotic areas Message-ID: <7A00E2723D55D311AC240000F831866802606F1C@vhaamaexc1.amarillo.med.va.gov> Does anyone have any suggestions as to how to successfully cut sections from skin lesions with keratotic areas (from the paraffin block)? Our pathologist has concerns that is not cutting right, therefore it is not staining correctly. We have tried everything we can think of. Any suggestions? We use low profile disposible blades, cutting at 5 micrometers using Microm microtomes. Thanks From shive003 <@t> umn.edu Tue Mar 29 11:16:04 2005 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Target retrieval solution References: <20050329162645.87101.qmail@web52602.mail.yahoo.com> Message-ID: <005101c53482$fd0a7140$41065486@auxs.umn.edu> In my experience, the time in Target Retrieval solution (of any type/brand) varies, depending upon the difficulty of exposing specific antigenic sites. Each antigen will need to be worked up individually, by comparing staining results of different time lengths in HIER with TR. I run mine at 10, 15, or 20 minutes.. depending upon the antigen in question, if you want a general answer. Jan Shivers U of MN ----- Original Message ----- From: "Larry Woody" To: Sent: Tuesday, March 29, 2005 10:26 AM Subject: [Histonet] Target retrieval solution > Dako makes a 10x target retrieval solution that a lot of people use in the decloaker but I've heard so many different times being used; ie; 20 min, 10 min, 2 min. or does it matter? suggestions. Larry W. > > > --------------------------------- > Do you Yahoo!? > Yahoo! Small Business - Try our new resources site! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lynne.Bell <@t> hitchcock.org Tue Mar 29 11:31:12 2005 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Metal Embedding Molds Message-ID: We clean our molds weekly in the clean cycle of the tissue processor and then we spray lightly with mold release. Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 From katri <@t> cogeco.ca Tue Mar 29 12:00:33 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] ERK antibody References: Message-ID: <000401c53489$34095d40$6a9a9618@Katri> Hi Yan, I have done some phospho-ERK IHC on FFPE human lung tumours. My antibody is from Cell Signaling Technology; rabbit monoclonal phospho-p44/42 MAPK (p44 is Erk1 and P42 is Erk2 according to the specification sheet). If your antibody is from another company, my protocol may not work on yours. I do HIER in citrate buffer after blocking endogenous peroxidases. After normal serum blocking, incubation with primary antibody (dilution 1:100) is done overnight at 4C. My secondary system is Vector Elite ABC kit. Chromogen DAB. Some lung tumour cells show nuclear positivity, but a variety of normal cells are positive also. I hope this helps a bit... Katri Katri Tuomala Hamilton, Ontario, Canada t From katri <@t> cogeco.ca Tue Mar 29 12:21:26 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Target retrieval solution References: <20050329162645.87101.qmail@web52602.mail.yahoo.com> <005101c53482$fd0a7140$41065486@auxs.umn.edu> Message-ID: <003001c5348c$1e95b280$6a9a9618@Katri> Jan and everyone else using the decloaker: The Biocare decloaker has two stages: SP1 and SP2. When you say running time is 10, 15 or 20 minutes, is it total of two stages or just the SP2 stage? If I remember correctly SP1 can only be set maximum 5 minutes. Could someone clarify this for me? Katri Katri Tuomala Hamilton, Ontario, Canada From mrsgbd2001 <@t> yahoo.com Tue Mar 29 13:22:13 2005 From: mrsgbd2001 <@t> yahoo.com (Gareth Davis) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Metal Embedding Molds In-Reply-To: 6667 Message-ID: <20050329192213.14976.qmail@web52707.mail.yahoo.com> We actually place all the molds in our oven and let the paraffin melt off, then we run them through the purge cycle. We do this everyday. Makes for better release, but we have never used mold release. Gareth Davis Histotech PathGroup, Nashville, Tn --- David_S._Ahmed/HOU/UTMDACC@mdanderson.org wrote: > For those labs using the metal embedding molds, how > often do you clean > them with xylene and alcohol? > > David Ahmed > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From shive003 <@t> umn.edu Tue Mar 29 13:23:59 2005 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Target retrieval solution References: <20050329162645.87101.qmail@web52602.mail.yahoo.com> <005101c53482$fd0a7140$41065486@auxs.umn.edu> <003001c5348c$1e95b280$6a9a9618@Katri> Message-ID: <006501c53494$dc000480$41065486@auxs.umn.edu> Sorry... I was writing solely about Target Retrieval solution, and not about the Decloaker in particular, in my previous email. I perform HIER in a microwave, where I can set any time I want. The 10-15-20 minutes pertains to the heating time only. There is also the 20 minute cooldown in solution afterward, independent of the timing of the heating length. I also have a Biocare Decloaker which I use for CWD/Scrapie tissues only. The SP1 is the heating time length at high pressure; once the decloaker reaches the set temperature/pressure, the time countdown starts; the time can be adjusted up or down. The SP2 is the depressurizing step. My decloaker is set for 20 minutes at SP1, and 25 minutes at SP2. Jan Shivers U of MN ----- Original Message ----- From: "Katri Tuomala" To: "Jan Shivers" ; "Larry Woody" Cc: "histonet" Sent: Tuesday, March 29, 2005 12:21 PM Subject: Re: [Histonet] Target retrieval solution > Jan and everyone else using the decloaker: > The Biocare decloaker has two stages: SP1 and SP2. When you say running time > is 10, 15 or 20 minutes, is it total of two stages or just the SP2 stage? If > I remember correctly SP1 can only be set maximum 5 minutes. Could someone > clarify this for me? > Katri > > Katri Tuomala > Hamilton, Ontario, Canada > > From jqb7 <@t> cdc.gov Tue Mar 29 13:29:45 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Metal Embedding Molds Message-ID: We have been told that we cannot place our base molds in the processor (and we only do it twice a year) because it supposedly causes problems when using these cleaning reagents for recycling. Has anyone else heard of this or had this problem? We have a B/R recycler. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Davis Sent: Tuesday, March 29, 2005 2:22 PM To: David_S._Ahmed/HOU/UTMDACC@mdanderson.org; Histonet Subject: Re: [Histonet] Metal Embedding Molds We actually place all the molds in our oven and let the paraffin melt off, then we run them through the purge cycle. We do this everyday. Makes for better release, but we have never used mold release. Gareth Davis Histotech PathGroup, Nashville, Tn --- David_S._Ahmed/HOU/UTMDACC@mdanderson.org wrote: > For those labs using the metal embedding molds, how > often do you clean > them with xylene and alcohol? > > David Ahmed > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Tue Mar 29 13:32:26 2005 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Job Change Completed Message-ID: <1112124746.4249ad4aaabcb@imp.vet.upenn.edu> Hi All, Well, I made the change and left Polysciences at the beginning of this month to return to research at the University of Pennsylvania. I am now in an orthopedic and general histology research laboratory. I am doing MMA, GMA, epoxy (for light and EM) and paraffin processing, embedding and sectioning for routine staining and IHC. We do mineralized and/or decal bone, bio-structures and soft tissues for internal groups and outside clients. When I started we had a number of studies in-house to work on and finish. I am busy!! However, we are always looking for more to do and challenge us with studies outside the University. Partnering with other laboratories for collaboration or consulting would be welcome and we are open to new ideas. Over the next few months we will be binging in students from the University and (we hope) looking for additional histologists to learn plastics and grow with us. This is really exciting to me. If anyone needs any of the services we are offering for overflow or new projects let me know and we will be happy to talk to you and attempt to work them in. Pam Marcum Senior Research Technican University of Pennsylvania School of Veterinary Medicine R S Reynolds Jr Comparative Orthopedic Research Laboratory New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 From vazquezr <@t> ohsu.edu Tue Mar 29 13:33:20 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Metal Embedding Molds Message-ID: David, We use to just throw them in the clean cycle of the processor... Robyn OHSU >>> 03/29/05 7:14 AM >>> For those labs using the metal embedding molds, how often do you clean them with xylene and alcohol? David Ahmed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From psrice <@t> u.arizona.edu Tue Mar 29 13:40:19 2005 From: psrice <@t> u.arizona.edu (psrice@u.arizona.edu) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Grinding MMA Message-ID: <20050329124019.l8qosgskc4g4k0cg@www.email.arizona.edu> I am working with MMA and I am aware that it is toxic in certain forms, however when grinding polymerized blocks are the fumes toxic? Should I even be smelling fumes? Thanks Faith Rice From Charles.Embrey <@t> carle.com Tue Mar 29 14:07:43 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] New PA info from BOR Message-ID: There is now new info for PA's on the BOR site. I did notice that we were not given a separate category but have been lumped in with "specialists" http://www.ascp.org/bor/application/procedures/index.asp . The application requirements are now also listed http://www.ascp.org/bor/application/procedures/pa.asp . I did notice that there is no mention of OJT phase out so I don't know if this will come later if ever. Chuck From Charles.Embrey <@t> carle.com Tue Mar 29 14:24:56 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] New PA info from BOR Message-ID: I need to make a quick correction. The OJT window is closing. Sorry, I missed the note at the bottom of the page. "NOTE: Route 2 will expire effective December 31, 2007. After that date, all candidates will be required to complete a NAACLS accredited Pathologists' Assistant Program." Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Tuesday, March 29, 2005 2:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New PA info from BOR There is now new info for PA's on the BOR site. I did notice that we were not given a separate category but have been lumped in with "specialists" http://www.ascp.org/bor/application/procedures/index.asp . The application requirements are now also listed http://www.ascp.org/bor/application/procedures/pa.asp . I did notice that there is no mention of OJT phase out so I don't know if this will come later if ever. Chuck _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Thomas.Amparano <@t> hcs.maricopa.gov Tue Mar 29 14:27:25 2005 From: Thomas.Amparano <@t> hcs.maricopa.gov (Thomas Amparan) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] proper disposal of specimen transport biohazard bags Message-ID: <88BCF05B75BAEB4FB121C66E6484F0515D7B76@app-msg02> I read the comments by Gervaip@aol.com on 13 Aug 2004 19:28:50 EDT, regarding this issue, but I was wondering if there is any documentation which states that specimen bags with a biohazard symbol my be disposed if in regular trash. I do understand that the by definition an empty transport bag does not meet the criteria for Regulated Medical Waste. The OSHA standard 1910.1030 does not spell this out clearly. Tommy Amparano From azdudley <@t> hotmail.com Tue Mar 29 13:16:00 2005 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Pneumocystis carnii staining In-Reply-To: <000601c533e5$0f9c0e20$12dca643@CPQ20882111713> Message-ID: karen, in our lab we have found that we have to over stain the gms to get the pneumo to demonstrate. hope this helps. anita dudley, providence hosp. mobile alabama >From: "Karen Harmon" >To: >Subject: [Histonet] Pneumocystis carnii staining >Date: Mon, 28 Mar 2005 16:25:33 -0600 > >We use a GMS stain to demonstrate pneuomcyctis with successful results >about 50% of the time. We have taken this procedure apart step by step, >solution by solution and are at our wits end to determine the cause for >failure. I can assure you that over the past 3 weeks, there is no variable >that we have not tried. The ironic part is we never have a problem >demonstrating fungi with the GMS, in fact, we never had any problems at all >with other silver procedures. > >Can anyone help with our hit and miss problem with pneumocystis? > >Karen Harmon >HT (ASCP) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Mar 29 14:33:42 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Re: Grinding MMA In-Reply-To: <20050329124019.l8qosgskc4g4k0cg@www.email.arizona.edu> References: <20050329124019.l8qosgskc4g4k0cg@www.email.arizona.edu> Message-ID: <6.0.0.22.1.20050329133127.01b2b620@gemini.msu.montana.edu> How are you grinding the blocks, on a water cooled grinder/polisher? The fumes of methyl methacrylate monomer are toxic particularly to the central nervous systemAt 12:40 PM 3/29/2005, you wrote: >I am working with MMA and I am aware that it is toxic in certain >forms, however >when grinding polymerized blocks are the fumes toxic? Should I even be >smelling >fumes? >Thanks > Faith Rice > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From SCheasty <@t> memorialcare.org Tue Mar 29 14:38:11 2005 From: SCheasty <@t> memorialcare.org (Sandy Cheasty) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Pneumocystis carnii staining Message-ID: <59FE4C55358C6A42B84BAEC6092CEDBC05ACF5F6@sbnt7> Karen, We recently had the same problem and solved it using the SIGMA Meth Silver Kit but used Chromic Acid instead of periodic acid. (We microwave the meth-silver step) Delicate, clear, reliable staining with zero back ground. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, March 29, 2005 11:16 AM To: karenharmon@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pneumocystis carnii staining karen, in our lab we have found that we have to over stain the gms to get the pneumo to demonstrate. hope this helps. anita dudley, providence hosp. mobile alabama >From: "Karen Harmon" >To: >Subject: [Histonet] Pneumocystis carnii staining >Date: Mon, 28 Mar 2005 16:25:33 -0600 > >We use a GMS stain to demonstrate pneuomcyctis with successful results >about 50% of the time. We have taken this procedure apart step by step, >solution by solution and are at our wits end to determine the cause for >failure. I can assure you that over the past 3 weeks, there is no variable >that we have not tried. The ironic part is we never have a problem >demonstrating fungi with the GMS, in fact, we never had any problems at all >with other silver procedures. > >Can anyone help with our hit and miss problem with pneumocystis? > >Karen Harmon >HT (ASCP) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Sign up for your free MemorialCare Medical Information and Access Card at http://www.memorialcare.com/apps/AccessCard/AboutCard.cfm From weneng2004 <@t> yahoo.com Tue Mar 29 15:50:22 2005 From: weneng2004 <@t> yahoo.com (wen eng) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Orcein Message-ID: <20050329215023.64894.qmail@web53402.mail.yahoo.com> Hi, I was asked to do Orcein stain for elastic fibres. I couldn't find method. Could anybody tell me where I can find it or send me a copy? I really apperciate any input. Wen --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From jnocito <@t> satx.rr.com Tue Mar 29 19:26:53 2005 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Job Change Completed References: <1112124746.4249ad4aaabcb@imp.vet.upenn.edu> Message-ID: <001901c534c7$8fc7f630$7929f318@yourxhtr8hvc4p> Great Pam, I won't make any bones about it though. Joe "The Toe" ----- Original Message ----- From: To: Sent: Tuesday, March 29, 2005 1:32 PM Subject: [Histonet] Job Change Completed > Hi All, > > Well, I made the change and left Polysciences at the beginning of this > month to > return to research at the University of Pennsylvania. I am now in an > orthopedic > and general histology research laboratory. I am doing MMA, GMA, epoxy > (for > light and EM) > and paraffin processing, embedding and sectioning for routine staining and > IHC. > We do mineralized > and/or decal bone, bio-structures and soft tissues for internal groups and > outside > clients. > > When I started we had a number of studies in-house to work on and finish. > I am > busy!! However, we are > always looking for more to do and challenge us with studies outside the > University. Partnering > with other laboratories for collaboration or consulting would be welcome > and we > are open to new ideas. > Over the next few months we will be binging in students from the > University and > (we hope) looking for > additional histologists to learn plastics and grow with us. This is > really > exciting to me. > > If anyone needs any of the services we are offering for overflow or new > projects > let me > know and we will be happy to talk to you and attempt to work them in. > > Pam Marcum > Senior Research Technican > University of Pennsylvania > School of Veterinary Medicine > R S Reynolds Jr Comparative Orthopedic Research Laboratory > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > Phone - 610-925-6278 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.8.4 - Release Date: 3/27/2005 > > From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 30 01:39:16 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Cutting Skin Lesions with keratotic areas[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F0F8@bhrv-nt-11.bhrv.nwest.nhs.uk> Processing is the answer; most things will eventually cut if they are adequately processed. You may like to try 'double embedding' or other similar techniques; if you wish for further advice then don't hesitate. -----Original Message----- From: John.Thweatt@med.va.gov [mailto:John.Thweatt@med.va.gov] Sent: 29 March 2005 17:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting Skin Lesions with keratotic areas[Scanned] Importance: High Does anyone have any suggestions as to how to successfully cut sections from skin lesions with keratotic areas (from the paraffin block)? Our pathologist has concerns that is not cutting right, therefore it is not staining correctly. We have tried everything we can think of. Any suggestions? We use low profile disposible blades, cutting at 5 micrometers using Microm microtomes. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jerry.lyons <@t> ucd.ie Wed Mar 30 03:51:58 2005 From: jerry.lyons <@t> ucd.ie (Jerry Lyons) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Immunofluorescence and background Message-ID: <8362520.1112176318135.JavaMail.jerry.lyons@ucd.ie> Hi Histonetters, My project is based on breaking immune tolerance to the self-antigen flk-1 for tumour therapy. I am now trying to detect antibodies to flk-1 in whole mouse serum by immunofluorescence. The Flk-1 receptor is expressed on transfected BHK cells and I incubate the mouse serum at dilutions of 1:50, 1:100, and 1:200 with the cells overnight at 4 degrees celcius. There are positive cells but the background is really obscuring the whole picture. I would very much appreciate if anyone felt they could offer suggestions on eliminating the background on the immunofluorescence. Thanking you in advance. Jerry Lyons From arvind <@t> nbrc.res.in Wed Mar 30 05:38:32 2005 From: arvind <@t> nbrc.res.in (Arvind Pundir) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] gelatin embedding Message-ID: <7DE0291A47C4BC4D9CDEF65D5017CEF0A729@mail.nbrc.res.in> can any one please give detail of how to embed tissue block in gelatin arvind singh pundir NBRC , Deptt. of Neuroanatomy, Haryana, INDIA From mab70 <@t> medschl.cam.ac.uk Wed Mar 30 05:48:34 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Orcein Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114CA@mius2.medlan.cam.ac.uk> Dear Wen, Here is the method from my old favourite staining book, reference; Manual of Histological Demonstration Techniques H.C. Cook, Butterworths, 1974, ISBN 0 407 74700 1. Note that Orcein dye batches vary in quality and as a general rule, synthetic orcein is better than natural orcein. Dissolve 1g orceing in 100ml of 70% ethanol with the aid of gentle heat (waterbath!). Cool, filter and add 1ml of concentrated hydrochloric acid. Method: 1. take sections to 70% ethanol 2. Stain with orcein for 1 to 2 housr at 37C 3. Rinse with 70% ethanol and differentiate in 1% acid alcoholif necessary then wash well in water 4. Counterstain as required, suitable counterstains are Haematoxylin, 0.1% aqueous azure A or methylene blue for 1 to 2 minutes. 5. Dehydrate, clear and mount. Results Elastin dark brown Background according to the counterstain. NOTE: when I used this method the elastin gave a weak pale brown stain and I prefer the method in the following reference: Roman et al. (1967) Orcein-Haematoxyoin in iodides ferric chloride as a stain for elastic fibres with metanil yellow counterstaining. Stain Technology 42: 199. I ave attached a copy of my nethod card below. This is a nice method and works very well, picking up very fine elastic fibres. Good luck Margaret -----Original Message----- From: wen eng [mailto:weneng2004@yahoo.com] Sent: Tuesday, March 29, 2005 10:50 PM To: histonet Subject: [Histonet] Orcein Hi, I was asked to do Orcein stain for elastic fibres. I couldn't find method. Could anybody tell me where I can find it or send me a copy? I really apperciate any input. Wen --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.A.Harper <@t> pcola.med.navy.mil Wed Mar 30 06:27:30 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Microtome Adjuster Message-ID: <807FE48C5A7CC940B973B58D32E7014318F93AA9@nhpens-exch1.pcola.med.navy.mil> I have an old RMC MT-910 microtome. It seriously needs aligning and no service dept. will do it because it is such an old microtome. I have seen a lot of catalogs selling these aligners, and would like to know if this would help my old microtome or if I just need to go fight to get a new microtome. Any information on the microtome aligner will be deeply appreciated. The costs of this small metal gadget is pricey...whopping $775. Thank you in advance. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL From BMolinari <@t> heart.thi.tmc.edu Wed Mar 30 06:58:56 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Liz Wilson Message-ID: Hi , I am trying to contact Liz Wilson. At one time she worked at the UT Path Dept. If anyone has contact info for her would you let me know. Thanks, Betsy Molinari , HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, Texas 77030 832-355-6524 832-355-6812 (fax) From Kemlo.Rogerson <@t> elht.nhs.uk Wed Mar 30 07:04:14 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] gelatin embedding[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F106@bhrv-nt-11.bhrv.nwest.nhs.uk> Make up gelatine: Gelatine 16g Glycerine 15 ml Dist water 70 ml Thymol 1 crystal Heat gelatine in warm water (not too hot) until it dissolves, add glycerine then thymol when cool. Fix tissue in 10 per cent formalin (buffered) then wash in running tap water overnight (the fixative will fix the gelatine otherwise), place in fresh gelatine/ glycerine mixture at 37 degrees for 6 hours. Finally place in fresh gelatine/ glycerine mixture and cool in refrigerator. Trim block fix in 10 per cent buffered formalin overnight and store in fixative. Cut sections on a freezing microtome; really good fun. I used to enjoy fiddling with these things as a pup. -----Original Message----- From: Arvind Pundir [mailto:arvind@nbrc.res.in] Sent: 30 March 2005 12:39 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gelatin embedding[Scanned] can any one please give detail of how to embed tissue block in gelatin arvind singh pundir NBRC , Deptt. of Neuroanatomy, Haryana, INDIA From Heather.A.Harper <@t> pcola.med.navy.mil Wed Mar 30 08:05:25 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Tissue Tek Auto Tec Message-ID: <807FE48C5A7CC940B973B58D32E7014318F93ACC@nhpens-exch1.pcola.med.navy.mil> I have a Tissue Tek VIP 5 and was looking on their website. Granted histology has come a long ways as far as automation, but has anybody heard of this new automatic embedding center (robotic) called Tissue Tek Auto Tec by Sakura? I would love to see how and if embedding can truly be replaced by human hands. I can only imagine the cost. Supposedly it can embed 120 specimens per hour, can differentiate between, biopsy, standard and orientation. Now I find this extremely fascinating and would love to observe this machine. http://www.sakura-americas.com/readallabout.html It is the third link down on page if anyone wants to check this out. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL. From JNocito <@t> Pathreflab.com Wed Mar 30 08:12:28 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX From jqb7 <@t> cdc.gov Wed Mar 30 08:14:38 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Tissue Tek Auto Tec Message-ID: Contact your rep. and have them send out literature. I have seen it and it is great. The pathologist orients the tissue when grossing and places it in a special cassette that clamps down and holds it in place. This plastic screen is "cuttable" so you section right through it. I was given some of these special cassettes and hand embedded them just to see how they would cut and it was great. Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Wednesday, March 30, 2005 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Tek Auto Tec I have a Tissue Tek VIP 5 and was looking on their website. Granted histology has come a long ways as far as automation, but has anybody heard of this new automatic embedding center (robotic) called Tissue Tek Auto Tec by Sakura? I would love to see how and if embedding can truly be replaced by human hands. I can only imagine the cost. Supposedly it can embed 120 specimens per hour, can differentiate between, biopsy, standard and orientation. Now I find this extremely fascinating and would love to observe this machine. http://www.sakura-americas.com/readallabout.html It is the third link down on page if anyone wants to check this out. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Mar 30 08:26:12 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Jobs in Oz - for Oz members only Message-ID: I'm sure I asked a couple of weeks ago, but ............. one of ours techs has a mind to emigrate but has no idea how to go about finding a job there. Where are jobs advertised? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From dellav <@t> musc.edu Wed Mar 30 08:34:32 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Microtome Adjuster Message-ID: Heather, I'm not sure if the 'aligner' I have is the device you need since it isn't clear from your message exactly what your problem is. I purchased a block face aligner from Newcomer Supply. it allows us to keep the block faces of seven machines all the same so that a block cut by one individual can easily be re-cut by someone else. I believe the price we paid is what you quoted in your message or close to it. I don't understand what your machine being old has to do with adjusting the block face, which is what prompts me to question whether we are talking about the same device. in any event, the device I have works great and does what we need. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> 03/30/05 07:27AM >>> I have an old RMC MT-910 microtome. It seriously needs aligning and no service dept. will do it because it is such an old microtome. I have seen a lot of catalogs selling these aligners, and would like to know if this would help my old microtome or if I just need to go fight to get a new microtome. Any information on the microtome aligner will be deeply appreciated. The costs of this small metal gadget is pricey...whopping $775. Thank you in advance. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Wed Mar 30 08:46:55 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BRaiford <@t> nctr.fda.gov Wed Mar 30 09:17:35 2005 From: BRaiford <@t> nctr.fda.gov (Raiford, Betty*) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Opportunity for Histology Laboratory Manager Message-ID: HISTOLOGY LABORATORY MANAGER Toxicologic Pathology Associates, at the National Center for Toxicological Research in Jefferson, AR is recruiting for a Histology Laboratory Manager. The Histology Laboratory Manager will work directly with the Program Director and the rest of the Pathology Services Project team in our veterinary biomedical research laboratory. The selected candidate must have HT (ASCP certification), at least 5 years of hands-on histologic supervisory experience, familiarity in all aspects of the operation and management of a histology laboratory and staff and knowledge and experience with current technologies. Experience in Good Laboratory Practice (GLP) laboratory environment is highly desirable. BS degree preferred. We offer competitive salary, good benefits package, generous paid time off plan and much more! Interested candidates can submit their resume to: Dr. Paul Mellick, Program Director, Toxicologic Pathology Associates, 3900 NCTR Drive, Jefferson, AR 72079, Phone: (870)543-7022; Fax: (870)543-7401 Email: pmellick@nctr.fda.gov . EOE M/F/DV Betty Raiford Program Manager Toxicologic Pathology Associates 3900 NCTR Rd. Jefferson, AR 72079 phone (870)543-7035 fax (870)543-7401 braiford@nctr.fda.gov CONFIDENTIALITY NOTICE - This email is ONLY for the person(s) named in the message header. Unless otherwise indicated, it contains information that is confidential, privileged or exempt from disclosure under applicable law. If you have received this email in error, please notify the sender of the error and delete the entire message. Thank you. From Allison_Scott <@t> hchd.tmc.edu Wed Mar 30 09:57:53 2005 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] Broken glass and Paraffin Block Disposal Message-ID: If anyone has a procedure for the disposal of used glass and paraffin blocks that they are willing to share, I would greatly appreciate it. We are being surveyed by CAP in 2 weeks. This is in regards to ANP.27150 on the AP checklist. Thanks in advance. Allison Scott HT(ASCP) LBJ Hospital 5656 Kelley Houston, Texas 77026 fax: 7135665285 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From vazquezr <@t> ohsu.edu Wed Mar 30 09:59:42 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:50 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Wed Mar 30 10:02:50 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: No it is not. Texians is what the heroes of the Alamo called themselves. It was probably a combination of Texan and Mexican. Yes there was some Mexicans fighting for Texas freedom. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: Wednesday, March 30, 2005 10:00 AM To: GUTIERREZ, JUAN; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Mar 30 10:04:36 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA457CF@sjhaexc02.sjha.org> It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From krat18 <@t> aol.com Wed Mar 30 10:10:14 2005 From: krat18 <@t> aol.com (krat18@aol.com) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese In-Reply-To: References: Message-ID: <8C7035A17B0CA5F-880-2231A@mblk-r36.sysops.aol.com> Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com From Barry.R.Rittman <@t> uth.tmc.edu Wed Mar 30 10:15:05 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0015C05C2@UTHEVS3.mail.uthouston.edu> Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From JWEEMS <@t> sjha.org Wed Mar 30 10:18:55 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA457D0@sjhaexc02.sjha.org> I'll have to hear it so I can be accepted if I ever go there! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Wednesday, March 30, 2005 11:15 AM To: histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From mauger <@t> email.chop.edu Wed Mar 30 10:20:39 2005 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ERK antibody Message-ID: Hi Yan, I have had success with the Phospho-p44/42 Map Kinase antibody from Cell Signaling Technology-www.cellsignal.com. It stains both Erk1 and Erk2. Order # is 9101 I use it at 1:50 dilution for 1 hour. For antigen retrieval, use pH 10 buffer. Good luck, Jo >>> "yan gao" 03/28/05 1:24 PM >>> Dear Histonet, Does anyone done phospho-ERK antibody? I am working on the RAF pathway need to stain pERK IHC in human tumor paraffin section. Can anyone give some tips? THank a lot. Yan Gao Novartis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Mar 30 10:24:16 2005 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3CAC@exchsrv01.barrynet.barry.edu> Col. Travis, the Texian commander at the Alamo, insisted that he was fighting only for the restoration of constitutional government in Mexico (i.e. the Constitution of 1824). After his death, the war became a war for independence. [This is similar to the U.S. and Haitian wars of independence, which started out as wars to secure constitutional rights and became wars of independence only when the colonial power escalated its efforts to deny those rights.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From pedro.louro <@t> spcorp.com Wed Mar 30 07:59:51 2005 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Leder Stain Message-ID: <4508920F80C0D411B90200508BF9A9F4062B48DE@LAFMSG30.us.schp.com> I'm looking for information on "Leder Stain" (chloracetate esterase activity)...looking to stain neutrophils on FFPE mouse tissue If someone can supply me with the recipe or the ingredients would be great Thanks Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From pedro.louro <@t> spcorp.com Wed Mar 30 08:06:33 2005 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] CD15 Ab question Message-ID: <4508920F80C0D411B90200508BF9A9F4062B48DF@LAFMSG30.us.schp.com> I'm trying to find a CD15 antibody that will work on FFPE mouse tissue. Looked all over without any success. Would appreciate any information on this matter. Thanks in advance, Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From krat18 <@t> aol.com Wed Mar 30 10:36:33 2005 From: krat18 <@t> aol.com (krat18@aol.com) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Charging for immunos In-Reply-To: <8C7035A17B0CA5F-880-2231A@mblk-r36.sysops.aol.com> References: <8C7035A17B0CA5F-880-2231A@mblk-r36.sysops.aol.com> Message-ID: <8C7035DC46E333B-594-DD33@mblk-r16.sysops.aol.com> -----Original Message----- From: krat18@aol.com To: histonet@pathology.swmed.edu Sent: Wed, 30 Mar 2005 11:10:14 -0500 Subject: Re: [Histonet] ASCP whine. Please pass the cheese Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Wed Mar 30 10:39:24 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: Amen brother. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, March 30, 2005 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Texians, OT Col. Travis, the Texian commander at the Alamo, insisted that he was fighting only for the restoration of constitutional government in Mexico (i.e. the Constitution of 1824). After his death, the war became a war for independence. [This is similar to the U.S. and Haitian wars of independence, which started out as wars to secure constitutional rights and became wars of independence only when the colonial power escalated its efforts to deny those rights.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Mar 30 10:58:51 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] re: Texas Message-ID: <20050330165851.78704.qmail@web52610.mail.yahoo.com> If you look at a map of North America in an anatomical way you get a good view of where Texas stands in the big picture. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From David.Edmondson <@t> christie-tr.nwest.nhs.uk Wed Mar 30 10:59:08 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:51 2005 Subject: FW: [Histonet] Leder Stain Message-ID: Hi Have no idea whose name should be attributed to this but.. Slides to water. We have 30mg of Fast Blue BB base and 6mg of alpha Naphthyl Chloroacetate The first dissolved in 0.2M pH 7.6 TrisMalate buffer, the second in a few drops of Dimethyl Formamide and then added to the buffer. All is mixed and the slides immersed for 20-30mins. 37deg C is fine but go for a shorter time to avoid background. Counterstain say Neutral Red, rinse then dehydrate rapidly and mount. Take too long in alcohol or waiting to be mounted may abolish the staining. Check hazards. Do risk assessment To save calculations, could look in Bancroft and Stevens appendix for buffer table.where dissolve 2.42g TRIS + 2.32g Maleic acid in 100ml of water then 0.8g NaOH in 100ml of water 25ml of first plus 29ml of alkali give pH7.6 Dave Christie Hosp Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Louro, Pedro Sent: 30 March 2005 15:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leder Stain I'm looking for information on "Leder Stain" (chloracetate esterase activity)...looking to stain neutrophils on FFPE mouse tissue If someone can supply me with the recipe or the ingredients would be great Thanks Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Wed Mar 30 11:02:28 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] RE:Charging for immunos Message-ID: <20050330170228.81384.qmail@web30402.mail.mud.yahoo.com> Hi karen, My understanding of this reg is that you can only bill once for each specimen part. For example if you have a case such in multiple parts (i.e. a colon cancer with the colon as part one, a liver biopsy as part two and an abdominal wall met as part three) if you order S 100 on blocks A, B and C of part one you can only bill for one. But if you order an s 100 on a colon slide from part one and and s 100 on the liver biopsy from part two you can bill for each. I belive the same is true for special stains like AFB and fungal stains. ect. If anyone has different info I would appreciate your interpretation. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From juan.gutierrez <@t> christushealth.org Wed Mar 30 11:06:50 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] re: Texas Message-ID: That's right we got all the b.... DON'T MESS WITH TEXAS! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Wednesday, March 30, 2005 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: Texas If you look at a map of North America in an anatomical way you get a good view of where Texas stands in the big picture. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Wed Mar 30 11:19:28 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese In-Reply-To: Message-ID: Juan, I've lived in Texas longer than I've lived up north. Joe -----Original Message----- From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] Sent: Wednesday, March 30, 2005 8:47 AM To: Joe Nocito; Histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Wed Mar 30 11:22:15 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese In-Reply-To: <8C7035A17B0CA5F-880-2231A@mblk-r36.sysops.aol.com> Message-ID: Karen, I don't think it is a CAP requirement, I think it has something to do with Medicare/ Medicaid and the insurance companies for reimbursement. Hold on, I have to check my ICD-9 code book Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of krat18@aol.com Sent: Wednesday, March 30, 2005 10:10 AM To: histonet@pathology.swmed.edu Subject: Re: [Histonet] ASCP whine. Please pass the cheese Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Wed Mar 30 11:22:45 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese In-Reply-To: <566FB0B522443D43AF02D2ADBE35A6F0015C05C2@UTHEVS3.mail.uthouston.edu> Message-ID: Thank you Barry Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Wednesday, March 30, 2005 10:15 AM To: histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Wed Mar 30 11:30:11 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] re: Texas In-Reply-To: <20050330165851.78704.qmail@web52610.mail.yahoo.com> Message-ID: Hey Larry, where you from? I didn't see it on your email. If you look at Florida, anatomically speaking, you see the same result> Remember the Alamo!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Wednesday, March 30, 2005 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: Texas If you look at a map of North America in an anatomical way you get a good view of where Texas stands in the big picture. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Wed Mar 30 11:29:56 2005 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Re: TREATING B5 FIXED TISSUE BEFORE IHC'S Message-ID: <20050330.093055.1109.140913@webmail23.nyc.untd.com> Can someone share their procedure for treating B5 fixed tissue before performing Immunos please. Appreciate it. Marsha Price From juan.gutierrez <@t> christushealth.org Wed Mar 30 11:38:46 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Re: TREATING B5 FIXED TISSUE BEFORE IHC'S Message-ID: If you're doing HIER, you don't have to do anything to the slides. If not, de-mercurize as usual. Shouldn't you be switching away from mercury by now? Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mprice26@juno.com Sent: Wednesday, March 30, 2005 11:30 AM To: Allison_Scott@hchd.tmc.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: TREATING B5 FIXED TISSUE BEFORE IHC'S Can someone share their procedure for treating B5 fixed tissue before performing Immunos please. Appreciate it. Marsha Price _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Wed Mar 30 11:42:34 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Immunofluorescence in bone sections Message-ID: <20050330174234.68138.qmail@web15510.mail.cnb.yahoo.com> Hello,all, I am doing immunofluorescence in bone sections, but the results are not good, I do not know the reasons, can anybody do me a favor? My questions: 1. In bone sections, I found that some positions are stained, but some positions are not stained, I do not know why it appears. 2. In addition, this protein should be expressed strongly in bone marrow, but sometimes it is very weak, sometimes it is not stained. why? Thank you! Guofeng --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From kelly.mcqueeney <@t> bms.com Wed Mar 30 11:46:01 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese In-Reply-To: References: Message-ID: <424AE5D9.8060501@bms.com> I also love living up north. Believe it or not, the only time I hear about those pesky Yankees are when we are talking baseball...... Kelly Joe Nocito wrote: >Thank you Barry > >Joe > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, >Barry R >Sent: Wednesday, March 30, 2005 10:15 AM >To: histonet >Subject: RE: [Histonet] ASCP whine. Please pass the cheese > > >Joyce >Texian and Texicans are words used here, just like y'all and y'all >y'all. >These words have arisen because many of the northerners are unable to >understand the culture here and be accepted. Once you understand how to >pronounce these words and actually use them everyday then you understand >something about the culture here and you are accepted. >I can say this as I migrated from the north (Iowa) 15 years ago and, >like Joe, have loved every minute. >Barry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, >Joyce >Sent: Wednesday, March 30, 2005 10:05 AM >To: Robyn Vazquez; juan.gutierrez@christushealth.org; >histonet@pathology.swmed.edu; JNocito@Pathreflab.com >Subject: RE: [Histonet] ASCP whine. Please pass the cheese > >It just became one - hasn't had time to make it to publication yet, j:>) > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn >Vazquez >Sent: Wednesday, March 30, 2005 11:00 AM >To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; >JNocito@Pathreflab.com >Subject: RE: [Histonet] ASCP whine. Please pass the cheese > > >Juan, >Is Texians a new word? For the life of me can't find it in any >dictionary up North here!!! > >Robyn Vazquez >OHSU > > > >>>>"GUTIERREZ, JUAN" 03/30/05 6:46 >>>> >>>> >AM >>> >You know Joe, we Texians were annexed unconstitutionally. We should >just take up arms and re-revolt against the yankee oppressors( I know >you're a transplanted yankee, but we're willing to overlook it). If Key >West can secede why can't we? We were never formally asked to join the >United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER >THE ALAMO! > >Juan C. Gutierrez, HT(ASCP) >Histology Laboratory Supervisor >(210)704-2533 > >My opinions are my own and do not reflect those of my employer. Long >live free speech and the Republic of Texas! > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe >Nocito >Sent: Wednesday, March 30, 2005 8:12 AM >To: Histonet >Subject: [Histonet] ASCP whine. Please pass the cheese > >you might want to hit the delete button on this one. I'm prepared to get >flamed and have my registries revoked, but I have to get something off >my >chest. > As some of you may know, ASCP is now in charge of handling the >Pathologists' Assistant exam, of which I am partaking in. Not only did >they >raise the exam fee from $150 to $450, we now have to know ICD-9 codes, >not >to mention Robbin's Pathology book (which is heavy enough to break a >vase. I >know this because it fell out of my hand the other night and shattered a >vase.) Have you seen the ICD-9 book? Rather extension reading if I do >say so >myself. > Moving on, after January 2008, one has to be a graduate of an >accredited >program. Well, that's just dandy because we in the South do not have any >programs to attend because they are all up North or on the East Coast. >Are >we going to have to repeat the 1860's again? Are we in the South going >to >have to resort to revolting and have demonstration marches? Do I sense >some >discrimination here? Am I too sensitive? (I think not) > It took us two years here just to get a Histology program up and >going at >the local community college. How long is it going to take to start a >master's level program? > There, I've said my piece. thank you for letting me get thing >off my chest. >Oh yeah, I'm collecting money to replace the vase I broke. Until then, >I'll >be sleeping with my dogs. Which is okay, but the fleas are killing me. > One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). > as always, the opinion of this author does not reflect the >opinions of the >employees, their children, mothers, lawyers, etc, etc, etc. > > >Joe Nocito, BS, HT(ASCP) QIHC >Histology Manager >Pathology Reference Lab >San Antonio, TX > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message may >be privileged and is confidential information intended for the use of >the addressee listed above. If you are neither the intended recipient >nor the employee or agent responsible for delivering this message to the >intended recipient, you are hereby notified that any disclosure, >copying, distribution or the taking of any action in reliance on the >contents of this information is strictly prohibited. If you have >received this communication in error, please notify us immediately by >replying to the message and deleting it from your computer. >Thank you. Saint Josephs Health System, Inc. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Lynne.Bell <@t> hitchcock.org Wed Mar 30 12:02:11 2005 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: Pesky Yankees indeed!! GO RED SOX!!!! Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 From JNocito <@t> Pathreflab.com Wed Mar 30 12:14:04 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese In-Reply-To: Message-ID: okay Lynne, I have to go with you on that one. Also GO PATRIOTS!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bell, Lynne Sent: Wednesday, March 30, 2005 12:02 PM To: Kelly D Mcqueeney Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP whine. Please pass the cheese Pesky Yankees indeed!! GO RED SOX!!!! Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Wed Mar 30 12:15:02 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese In-Reply-To: Message-ID: See, I broke the Histonet, I'm getting duplicate responses. Only me, only me. Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 11:19 AM To: GUTIERREZ, JUAN; Histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, I've lived in Texas longer than I've lived up north. Joe -----Original Message----- From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] Sent: Wednesday, March 30, 2005 8:47 AM To: Joe Nocito; Histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbroomal <@t> NEMOURS.ORG Wed Mar 30 12:18:40 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Cryosectioning for LMD POL Slides Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A7970@wlmmsx01.nemours.org> One of the other techs here is cutting snap frozen muscle for LMD and is having quite a time getting the sections to pick up on the POL membrane of the slide. She's tried warming the membrane with her finger, and I'm sure a couple other tricks, but barely any tissue with stick to it. Certainly not enough to constitute a good section. We're using Leica POL Membrane, metal framed slides. Any suggestions? Thanks in advance! Kristen Broomall, HT (ASCP) A.I. DuPont Hospital for Children kbroomal@nemours.org > NOTICE...This electronic transmission is intended only for the person(s) > named. It may contain information that is (i) proprietary to the sender, > and/or (ii) privileged, confidential and/or otherwise exempt from > disclosure under applicable State and Federal law, including, but not > limited to, privacy standards imposed pursuant to the federal Health > Insurance Portability and Accountability Act of 1996 (HIPAA). Receipt by > anyone other than the named recipient(s) is not a waiver of any applicable > privilege. If you received this confidential communication in error, > please notify the sender immediately by reply e-mail message and > permanently delete the original message from your system. > > > From Julie.Sanders <@t> med.va.gov Wed Mar 30 12:19:07 2005 From: Julie.Sanders <@t> med.va.gov (Julie.Sanders@med.va.gov) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] CAP Inspection Message-ID: <457381D92B01BD44B21CF37CC02EBDFD029275BD@vhacinexc2.v10.med.va.gov> We are in the midst of our CAP interim inspection and I have some questions about new things on the checklist. 1. Procedure for validation of ISH probes? ANP.22956 2. Procedure for scoring results of ISH? ANP.22974 3. Accuracy of pipettes (a procedure and excatly how is this done?) ANP.23085 4. A procedure for checking thermometers (a thermometric device?) ANP.23090 5. A policy for batch controls review for IHC? ANP.22660 Any advice/help/procedures would be greatly appreciated. Thanks, Julie Julie Sanders Supervisor, Anatomic Pathology Cincinnati VAMC From jqb7 <@t> cdc.gov Wed Mar 30 12:15:02 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: They're only pesky when they come to the south. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Wednesday, March 30, 2005 1:02 PM To: Kelly D Mcqueeney Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP whine. Please pass the cheese Pesky Yankees indeed!! GO RED SOX!!!! Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurenerobinson <@t> hotmail.com Wed Mar 30 12:33:21 2005 From: laurenerobinson <@t> hotmail.com (Lauren Robinson) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] unsubscribe In-Reply-To: Message-ID: >From: "Joe Nocito" >To: "Histonet" >Subject: [Histonet] ASCP whine. Please pass the cheese >Date: Wed, 30 Mar 2005 08:12:28 -0600 > >you might want to hit the delete button on this one. I'm prepared to get >flamed and have my registries revoked, but I have to get something off my >chest. > As some of you may know, ASCP is now in charge of handling the >Pathologists' Assistant exam, of which I am partaking in. Not only did they >raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not >to mention Robbin's Pathology book (which is heavy enough to break a vase. I >know this because it fell out of my hand the other night and shattered a >vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so >myself. > Moving on, after January 2008, one has to be a graduate of an accredited >program. Well, that's just dandy because we in the South do not have any >programs to attend because they are all up North or on the East Coast. Are >we going to have to repeat the 1860's again? Are we in the South going to >have to resort to revolting and have demonstration marches? Do I sense some >discrimination here? Am I too sensitive? (I think not) > It took us two years here just to get a Histology program up and going at >the local community college. How long is it going to take to start a >master's level program? > There, I've said my piece. thank you for letting me get thing off my chest. >Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll >be sleeping with my dogs. Which is okay, but the fleas are killing me. > One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). > as always, the opinion of this author does not reflect the opinions of the >employees, their children, mothers, lawyers, etc, etc, etc. > > >Joe Nocito, BS, HT(ASCP) QIHC >Histology Manager >Pathology Reference Lab >San Antonio, TX > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STEGTM <@t> samcstl.org Wed Mar 30 11:38:38 2005 From: STEGTM <@t> samcstl.org (Therersa Stegall) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. From jqb7 <@t> cdc.gov Wed Mar 30 12:41:46 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: I've lived in the south all of my life and the only person I ever heard say "warsh" was from Minnesota! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therersa Stegall Sent: Wednesday, March 30, 2005 12:39 PM To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: Re: [Histonet] Texians, OT Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Mar 30 13:00:48 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO I LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting emails), I really liked this one. Before all the Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind that thumping one's chest about how great a region/state is sounds great to those who live there but gets old quite quickly to "us northerners/yankies/etc...". The whole "yankies" thing always did astound me since it implies lasting unrest over the civil war (which the south, by the way, lost). Time to drop that one. I'll hold off on saying how wonderful "the north" is or on the experience I have had with "the south" since I have no desire to start the Civil War II. One nation/indivisible sounds good to me. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Therersa Stegall [mailto:STEGTM@samcstl.org] Sent: Wednesday, March 30, 2005 11:39 AM To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: Re: [Histonet] Texians, OT Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RCHIOVETTI <@t> aol.com Wed Mar 30 13:11:46 2005 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Cryosectioning for LMD POL Slides Message-ID: <1e8.382a2902.2f7c53f2@aol.com> In a message dated 3/30/2005 11:19:40 AM US Mountain Standard Time, kbroomal@NEMOURS.ORG writes: > One of the other techs here is cutting snap frozen muscle for LMD and is > having quite a time getting the sections to pick up on the POL membrane of > the slide. She's tried warming the membrane with her finger, and I'm sure a > couple other tricks, but barely any tissue with stick to it. Certainly not > enough to constitute a good section. > > We're using Leica POL Membrane, metal framed slides. > > Kristen, A colleague of mine had exactly the same problem, with the same metal-framed slides. As I recall she tried coating the membranes with polylysine, and she also tried treating with a silanization agent (Dow Z-6040). I know there was some improvement after coating, but the process required some modifications because the film is so hydrophobic. I do believe she had to add some ethanol to the polylysine to get the film to wet properly. Details and concentrations are a little hazy...this was about a year ago. Contact me off-list if you need additional info on the exact procedure. I can put you in touch with the person who did the work. Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Independent Consultant for The Science, Technology and Industrial Sectors 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Member, Arizona Small Business Association - ASBA From Gururaj_Kalkeri <@t> vrtx.com Wed Mar 30 13:24:02 2005 From: Gururaj_Kalkeri <@t> vrtx.com (Gururaj_Kalkeri@vrtx.com) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Question about lipid quantitation in either frozen sections/paraffin blocks Message-ID: Dear All, I have some fatty liver tissue samples. They are already embedded in paraffin and OCT. They show some nice staining by Oil Red O. However, I want to quantitate the lipid in these tissue samples. I came across a paper- J.Histochem & Cytochem. 1988. Vol36, pp 1471-1474. However it uses fresh tissue blocks to quantitate fat by extraction and staining with Sudan IV and Fast Green. Can anybody let me know, how I can extract fat from either the paraffin/OCT embedded frozen blocks and quantitate fat spectophotometrically? I appreciate your help. Regards. Raj Kalkeri, PhD Infectious Diseases Biology 617-444-6275 From vazquezr <@t> ohsu.edu Wed Mar 30 13:30:03 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: I am from Oregon, and was in Texas in the late 80's at Fort Sam H and I loved it down there. I know "y'all" have some funny accents...:0) Robyn Vazquez OHSU >>> "Rittman, Barry R" 03/30/05 8:15 AM >>> Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Wed Mar 30 13:29:33 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: OK but I have found very few people here in Texas that can pronounce "Massachusetts" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Wednesday, March 30, 2005 12:42 PM To: Therersa Stegall; histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: RE: [Histonet] Texians, OT I've lived in the south all of my life and the only person I ever heard say "warsh" was from Minnesota! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therersa Stegall Sent: Wednesday, March 30, 2005 12:39 PM To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: Re: [Histonet] Texians, OT Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Wed Mar 30 13:29:57 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: -----Original Message----- From: Molinari, Betsy Sent: Wednesday, March 30, 2005 1:26 PM To: 'Bell, Lynne' Subject: RE: [Histonet] ASCP whine. Please pass the cheese I'll be at my home too(in Spring Texas, I am a Yankee and a damn Yankee at that!) Latest news is that Fenway is here to stay!!! Now that is something to WUHU about. Betsy MolinariHT (ASCP) Texas Heart Institute Houston,Texas -----Original Message----- From: Bell, Lynne [mailto:Lynne.Bell@hitchcock.org] Sent: Wednesday, March 30, 2005 12:17 PM To: Molinari, Betsy Subject: RE: [Histonet] ASCP whine. Please pass the cheese Home opener, banner raising and ring ceremony - gotta love it! I plan on leaving work early that day to be home to watch all the festivities - couldn't mortgage the house enough to get tickets to be at Fenway............. Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 From BMolinari <@t> heart.thi.tmc.edu Wed Mar 30 13:30:31 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: -----Original Message----- From: Molinari, Betsy Sent: Wednesday, March 30, 2005 12:08 PM To: 'Bell, Lynne' Subject: RE: [Histonet] ASCP whine. Please pass the cheese Lets not forget the NE Patriots!!!! Sox home opener..April 11th!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Wednesday, March 30, 2005 12:02 PM To: Kelly D Mcqueeney Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP whine. Please pass the cheese Pesky Yankees indeed!! GO RED SOX!!!! Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbroomal <@t> NEMOURS.ORG Wed Mar 30 13:35:54 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A7973@wlmmsx01.nemours.org> Good grief, how can one mispronounce Massachusetts? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Molinari, Betsy Sent: Wednesday, March 30, 2005 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Texians, OT OK but I have found very few people here in Texas that can pronounce "Massachusetts" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Wednesday, March 30, 2005 12:42 PM To: Therersa Stegall; histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: RE: [Histonet] Texians, OT I've lived in the south all of my life and the only person I ever heard say "warsh" was from Minnesota! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therersa Stegall Sent: Wednesday, March 30, 2005 12:39 PM To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: Re: [Histonet] Texians, OT Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Wed Mar 30 13:37:12 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: Juan, Theres nothing wrong with living up North, you just grow what we refer to is "web feet" They (feet) help us get around on the streets during the winter and on the lakes and beaches during the summer. Robyn OHSU >>> "Joe Nocito" 03/30/05 9:19 AM >>> Juan, I've lived in Texas longer than I've lived up north. Joe -----Original Message----- From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] Sent: Wednesday, March 30, 2005 8:47 AM To: Joe Nocito; Histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Wed Mar 30 13:40:53 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: West Virginian's say warsh, my grandma used to say it and she would also say y'll youngins'. Robyn >>> "Bartlett, Jeanine" 03/30/05 10:41 AM >>> I've lived in the south all of my life and the only person I ever heard say "warsh" was from Minnesota! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therersa Stegall Sent: Wednesday, March 30, 2005 12:39 PM To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: Re: [Histonet] Texians, OT Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Mar 30 16:54:26 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, even farther OT In-Reply-To: References: Message-ID: <424B2E22.5010209@umdnj.edu> In the South, it is not the Civil War, it is the War of Northern Aggression. Geoff (a yankee with some roots in western Tennessee) Dawson, Glen wrote: >Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO I >LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting >emails), I really liked this one. Before all the >Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind that >thumping one's chest about how great a region/state is sounds great to those >who live there but gets old quite quickly to "us >northerners/yankies/etc...". The whole "yankies" thing always did astound >me since it implies lasting unrest over the civil war (which the south, by >the way, lost). Time to drop that one. I'll hold off on saying how >wonderful "the north" is or on the experience I have had with "the south" >since I have no desire to start the Civil War II. One nation/indivisible >sounds good to me. > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: Therersa Stegall [mailto:STEGTM@samcstl.org] >Sent: Wednesday, March 30, 2005 11:39 AM >To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu >Subject: Re: [Histonet] Texians, OT > > >Sounds good to me. I've lived in Texas, and we should let them have >their independence, or return to Mexico, or whatever else the Texans, >Texians, or Texicans want to do. The place is too flat (except for the >rattlesnake infested mountains in the south), and too prone to tornados. > I can't stand local football fanatics, and cheerleaders, well, yuk. >I'm a Yankee who hasn't lived in the northeast since 1976, but I still >speak understandable english. There is no "r" in wash, and crouch is a >position, not a location. Enough, I'm starting to rant (again). St >Louis isalright, but I'd rather be in the Tetons. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From peoshel <@t> wisc.edu Wed Mar 30 13:53:13 2005 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT In-Reply-To: References: Message-ID: The Civil War is only over to us northerners. There are plenty in South who are still fighting it again. I imagine the whole exchange has been amusing to the non-US folks. To whom we are *all* Yankees, no matter how many syllables we put in "Mississippi". Phil >Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO I >LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting >emails), I really liked this one. Before all the >Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind that >thumping one's chest about how great a region/state is sounds great to those >who live there but gets old quite quickly to "us >northerners/yankies/etc...". The whole "yankies" thing always did astound >me since it implies lasting unrest over the civil war (which the south, by >the way, lost). Time to drop that one. I'll hold off on saying how >wonderful "the north" is or on the experience I have had with "the south" >since I have no desire to start the Civil War II. One nation/indivisible >sounds good to me. > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: Therersa Stegall [mailto:STEGTM@samcstl.org] >Sent: Wednesday, March 30, 2005 11:39 AM >To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu >Subject: Re: [Histonet] Texians, OT > > >Sounds good to me. I've lived in Texas, and we should let them have >their independence, or return to Mexico, or whatever else the Texans, >Texians, or Texicans want to do. The place is too flat (except for the >rattlesnake infested mountains in the south), and too prone to tornados. > I can't stand local football fanatics, and cheerleaders, well, yuk. >I'm a Yankee who hasn't lived in the northeast since 1976, but I still >speak understandable english. There is no "r" in wash, and crouch is a >position, not a location. Enough, I'm starting to rant (again). St >Louis isalright, but I'd rather be in the Tetons. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From froyer <@t> bitstream.net Wed Mar 30 14:13:36 2005 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese In-Reply-To: References: Message-ID: <424B0870.4060805@bitstream.net> Naw whole 'on dare jus a dogue gone minet... nuttin's wrng wid libbin up 'ear in da nord. 40 below kep da riff rraff out, done 'cha no now? Ohh 'ya u bet'cha.. ~ Ford Ford M. Royer, MT(ASCP) Minneapolis, MN ...where 'ya can't take a bath in the winter... water's too hard. Robyn Vazquez wrote: >Juan, >Theres nothing wrong with living up North, you just grow what we refer to is "web feet" They (feet) help us get around on the streets during the winter and on the lakes and beaches during the summer. > >Robyn >OHSU > > > >>>>"Joe Nocito" 03/30/05 9:19 AM >>> >>>> >>>> >Juan, >I've lived in Texas longer than I've lived up north. > >Joe > >-----Original Message----- >From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] >Sent: Wednesday, March 30, 2005 8:47 AM >To: Joe Nocito; Histonet >Subject: RE: [Histonet] ASCP whine. Please pass the cheese > > >You know Joe, we Texians were annexed unconstitutionally. We should just >take up arms and re-revolt against the yankee oppressors( I know you're a >transplanted yankee, but we're willing to overlook it). If Key West can >secede why can't we? We were never formally asked to join the United States >of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! > >Juan C. Gutierrez, HT(ASCP) >Histology Laboratory Supervisor >(210)704-2533 > >My opinions are my own and do not reflect those of my employer. Long live >free speech and the Republic of Texas! > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito >Sent: Wednesday, March 30, 2005 8:12 AM >To: Histonet >Subject: [Histonet] ASCP whine. Please pass the cheese > >you might want to hit the delete button on this one. I'm prepared to get >flamed and have my registries revoked, but I have to get something off my >chest. > As some of you may know, ASCP is now in charge of handling the >Pathologists' Assistant exam, of which I am partaking in. Not only did they >raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not >to mention Robbin's Pathology book (which is heavy enough to break a vase. I >know this because it fell out of my hand the other night and shattered a >vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so >myself. > Moving on, after January 2008, one has to be a graduate of an accredited >program. Well, that's just dandy because we in the South do not have any >programs to attend because they are all up North or on the East Coast. Are >we going to have to repeat the 1860's again? Are we in the South going to >have to resort to revolting and have demonstration marches? Do I sense some >discrimination here? Am I too sensitive? (I think not) > It took us two years here just to get a Histology program up and going at >the local community college. How long is it going to take to start a >master's level program? > There, I've said my piece. thank you for letting me get thing off my chest. >Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll >be sleeping with my dogs. Which is okay, but the fleas are killing me. > One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). > as always, the opinion of this author does not reflect the opinions of the >employees, their children, mothers, lawyers, etc, etc, etc. > > >Joe Nocito, BS, HT(ASCP) QIHC >Histology Manager >Pathology Reference Lab >San Antonio, TX > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From sbreeden <@t> nmda.nmsu.edu Wed Mar 30 14:03:31 2005 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians Message-ID: Only problem is, Texians don't know what CHILE is... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 30, 2005 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 49 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Opportunity for Histology Laboratory Manager (Raiford, Betty*) 2. Broken glass and Paraffin Block Disposal (Scott, Allison D) 3. RE: ASCP whine. Please pass the cheese (Robyn Vazquez) 4. RE: ASCP whine. Please pass the cheese (GUTIERREZ, JUAN) 5. RE: ASCP whine. Please pass the cheese (Weems, Joyce) 6. Re: ASCP whine. Please pass the cheese (krat18@aol.com) 7. RE: ASCP whine. Please pass the cheese (Rittman, Barry R) 8. RE: ASCP whine. Please pass the cheese (Weems, Joyce) 9. Re: ERK antibody (Joanne Mauger) 10. Texians, OT (Smith, Allen) 11. Leder Stain (Louro, Pedro) 12. CD15 Ab question (Louro, Pedro) 13. Charging for immunos (krat18@aol.com) 14. RE: Texians, OT (GUTIERREZ, JUAN) 15. re: Texas (Larry Woody) 16. FW: [Histonet] Leder Stain (Edmondson David (RBV) NHS Christie Tr) 17. RE:Charging for immunos (Stephen Peters M.D.) 18. RE: re: Texas (GUTIERREZ, JUAN) 19. RE: ASCP whine. Please pass the cheese (Joe Nocito) 20. RE: ASCP whine. Please pass the cheese (Joe Nocito) 21. RE: ASCP whine. Please pass the cheese (Joe Nocito) ---------------------------------------------------------------------- Message: 1 Date: Wed, 30 Mar 2005 09:17:35 -0600 From: "Raiford, Betty*" Subject: [Histonet] Opportunity for Histology Laboratory Manager To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain HISTOLOGY LABORATORY MANAGER Toxicologic Pathology Associates, at the National Center for Toxicological Research in Jefferson, AR is recruiting for a Histology Laboratory Manager. The Histology Laboratory Manager will work directly with the Program Director and the rest of the Pathology Services Project team in our veterinary biomedical research laboratory. The selected candidate must have HT (ASCP certification), at least 5 years of hands-on histologic supervisory experience, familiarity in all aspects of the operation and management of a histology laboratory and staff and knowledge and experience with current technologies. Experience in Good Laboratory Practice (GLP) laboratory environment is highly desirable. BS degree preferred. We offer competitive salary, good benefits package, generous paid time off plan and much more! Interested candidates can submit their resume to: Dr. Paul Mellick, Program Director, Toxicologic Pathology Associates, 3900 NCTR Drive, Jefferson, AR 72079, Phone: (870)543-7022; Fax: (870)543-7401 Email: pmellick@nctr.fda.gov . EOE M/F/DV Betty Raiford Program Manager Toxicologic Pathology Associates 3900 NCTR Rd. Jefferson, AR 72079 phone (870)543-7035 fax (870)543-7401 braiford@nctr.fda.gov CONFIDENTIALITY NOTICE - This email is ONLY for the person(s) named in the message header. Unless otherwise indicated, it contains information that is confidential, privileged or exempt from disclosure under applicable law. If you have received this email in error, please notify the sender of the error and delete the entire message. Thank you. ------------------------------ Message: 2 Date: Wed, 30 Mar 2005 09:57:53 -0600 From: "Scott, Allison D" Subject: [Histonet] Broken glass and Paraffin Block Disposal To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" If anyone has a procedure for the disposal of used glass and paraffin blocks that they are willing to share, I would greatly appreciate it. We are being surveyed by CAP in 2 weeks. This is in regards to ANP.27150 on the AP checklist. Thanks in advance. Allison Scott HT(ASCP) LBJ Hospital 5656 Kelley Houston, Texas 77026 fax: 7135665285 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 3 Date: Wed, 30 Mar 2005 07:59:42 -0800 From: "Robyn Vazquez" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: juan.gutierrez@christushealth.org, histonet@pathology.swmed.edu, JNocito@Pathreflab.com Message-ID: Content-Type: text/plain; charset=us-ascii Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 30 Mar 2005 10:02:50 -0600 From: "GUTIERREZ, JUAN" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "Robyn Vazquez" , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" No it is not. Texians is what the heroes of the Alamo called themselves. It was probably a combination of Texan and Mexican. Yes there was some Mexicans fighting for Texas freedom. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: Wednesday, March 30, 2005 10:00 AM To: GUTIERREZ, JUAN; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 30 Mar 2005 11:04:36 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "Robyn Vazquez" , , , Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA457CF@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 6 Date: Wed, 30 Mar 2005 11:10:14 -0500 From: krat18@aol.com Subject: Re: [Histonet] ASCP whine. Please pass the cheese To: histonet@pathology.swmed.edu Message-ID: <8C7035A17B0CA5F-880-2231A@mblk-r36.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com ------------------------------ Message: 7 Date: Wed, 30 Mar 2005 10:15:05 -0600 From: "Rittman, Barry R" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "histonet" Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0015C05C2@UTHEVS3.mail.uthouston.edu> Content-Type: text/plain; charset="us-ascii" Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 8 Date: Wed, 30 Mar 2005 11:18:55 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "Rittman, Barry R" , "histonet" Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA457D0@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" I'll have to hear it so I can be accepted if I ever go there! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Wednesday, March 30, 2005 11:15 AM To: histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 9 Date: Wed, 30 Mar 2005 11:20:39 -0500 From: "Joanne Mauger" Subject: Re: [Histonet] ERK antibody To: gliuygao@hotmail.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi Yan, I have had success with the Phospho-p44/42 Map Kinase antibody from Cell Signaling Technology-www.cellsignal.com. It stains both Erk1 and Erk2. Order # is 9101 I use it at 1:50 dilution for 1 hour. For antigen retrieval, use pH 10 buffer. Good luck, Jo >>> "yan gao" 03/28/05 1:24 PM >>> Dear Histonet, Does anyone done phospho-ERK antibody? I am working on the RAF pathway need to stain pERK IHC in human tumor paraffin section. Can anyone give some tips? THank a lot. Yan Gao Novartis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 30 Mar 2005 11:24:16 -0500 From: "Smith, Allen" Subject: [Histonet] Texians, OT To: Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3CAC@exchsrv01.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" Col. Travis, the Texian commander at the Alamo, insisted that he was fighting only for the restoration of constitutional government in Mexico (i.e. the Constitution of 1824). After his death, the war became a war for independence. [This is similar to the U.S. and Haitian wars of independence, which started out as wars to secure constitutional rights and became wars of independence only when the colonial power escalated its efforts to deny those rights.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) ------------------------------ Message: 11 Date: Wed, 30 Mar 2005 08:59:51 -0500 From: "Louro, Pedro" Subject: [Histonet] Leder Stain To: histonet@lists.utsouthwestern.edu Message-ID: <4508920F80C0D411B90200508BF9A9F4062B48DE@LAFMSG30.us.schp.com> Content-Type: text/plain I'm looking for information on "Leder Stain" (chloracetate esterase activity)...looking to stain neutrophils on FFPE mouse tissue If someone can supply me with the recipe or the ingredients would be great Thanks Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ Message: 12 Date: Wed, 30 Mar 2005 09:06:33 -0500 From: "Louro, Pedro" Subject: [Histonet] CD15 Ab question To: histonet@lists.utsouthwestern.edu Message-ID: <4508920F80C0D411B90200508BF9A9F4062B48DF@LAFMSG30.us.schp.com> Content-Type: text/plain I'm trying to find a CD15 antibody that will work on FFPE mouse tissue. Looked all over without any success. Would appreciate any information on this matter. Thanks in advance, Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ Message: 13 Date: Wed, 30 Mar 2005 11:36:33 -0500 From: krat18@aol.com Subject: [Histonet] Charging for immunos To: histonet@pathology.swmed.edu Message-ID: <8C7035DC46E333B-594-DD33@mblk-r16.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: krat18@aol.com To: histonet@pathology.swmed.edu Sent: Wed, 30 Mar 2005 11:10:14 -0500 Subject: Re: [Histonet] ASCP whine. Please pass the cheese Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 30 Mar 2005 10:39:24 -0600 From: "GUTIERREZ, JUAN" Subject: RE: [Histonet] Texians, OT To: "Smith, Allen" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Amen brother. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, March 30, 2005 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Texians, OT Col. Travis, the Texian commander at the Alamo, insisted that he was fighting only for the restoration of constitutional government in Mexico (i.e. the Constitution of 1824). After his death, the war became a war for independence. [This is similar to the U.S. and Haitian wars of independence, which started out as wars to secure constitutional rights and became wars of independence only when the colonial power escalated its efforts to deny those rights.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 30 Mar 2005 08:58:51 -0800 (PST) From: Larry Woody Subject: [Histonet] re: Texas To: histonet@lists.utsouthwestern.edu Message-ID: <20050330165851.78704.qmail@web52610.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii If you look at a map of North America in an anatomical way you get a good view of where Texas stands in the big picture. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! ------------------------------ Message: 16 Date: Wed, 30 Mar 2005 17:59:08 +0100 From: "Edmondson David \(RBV\) NHS Christie Tr" Subject: FW: [Histonet] Leder Stain To: "Histonet \(E-mail 2\)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Have no idea whose name should be attributed to this but.. Slides to water. We have 30mg of Fast Blue BB base and 6mg of alpha Naphthyl Chloroacetate The first dissolved in 0.2M pH 7.6 TrisMalate buffer, the second in a few drops of Dimethyl Formamide and then added to the buffer. All is mixed and the slides immersed for 20-30mins. 37deg C is fine but go for a shorter time to avoid background. Counterstain say Neutral Red, rinse then dehydrate rapidly and mount. Take too long in alcohol or waiting to be mounted may abolish the staining. Check hazards. Do risk assessment To save calculations, could look in Bancroft and Stevens appendix for buffer table.where dissolve 2.42g TRIS + 2.32g Maleic acid in 100ml of water then 0.8g NaOH in 100ml of water 25ml of first plus 29ml of alkali give pH7.6 Dave Christie Hosp Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Louro, Pedro Sent: 30 March 2005 15:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leder Stain I'm looking for information on "Leder Stain" (chloracetate esterase activity)...looking to stain neutrophils on FFPE mouse tissue If someone can supply me with the recipe or the ingredients would be great Thanks Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 30 Mar 2005 09:02:28 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] RE:Charging for immunos To: Histonet@lists.utsouthwestern.edu Message-ID: <20050330170228.81384.qmail@web30402.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi karen, My understanding of this reg is that you can only bill once for each specimen part. For example if you have a case such in multiple parts (i.e. a colon cancer with the colon as part one, a liver biopsy as part two and an abdominal wall met as part three) if you order S 100 on blocks A, B and C of part one you can only bill for one. But if you order an s 100 on a colon slide from part one and and s 100 on the liver biopsy from part two you can bill for each. I belive the same is true for special stains like AFB and fungal stains. ect. If anyone has different info I would appreciate your interpretation. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 ------------------------------ Message: 18 Date: Wed, 30 Mar 2005 11:06:50 -0600 From: "GUTIERREZ, JUAN" Subject: RE: [Histonet] re: Texas To: "Larry Woody" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" That's right we got all the b.... DON'T MESS WITH TEXAS! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Wednesday, March 30, 2005 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: Texas If you look at a map of North America in an anatomical way you get a good view of where Texas stands in the big picture. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 30 Mar 2005 11:19:28 -0600 From: "Joe Nocito" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "GUTIERREZ, JUAN" , "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Juan, I've lived in Texas longer than I've lived up north. Joe -----Original Message----- From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] Sent: Wednesday, March 30, 2005 8:47 AM To: Joe Nocito; Histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 30 Mar 2005 11:22:15 -0600 From: "Joe Nocito" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Karen, I don't think it is a CAP requirement, I think it has something to do with Medicare/ Medicaid and the insurance companies for reimbursement. Hold on, I have to check my ICD-9 code book Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of krat18@aol.com Sent: Wednesday, March 30, 2005 10:10 AM To: histonet@pathology.swmed.edu Subject: Re: [Histonet] ASCP whine. Please pass the cheese Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 30 Mar 2005 11:22:45 -0600 From: "Joe Nocito" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "Rittman, Barry R" , "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Thank you Barry Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Wednesday, March 30, 2005 10:15 AM To: histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 49 **************************************** From JWEEMS <@t> sjha.org Wed Mar 30 14:04:12 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA457E4@sjhaexc02.sjha.org> In east TN it's worsh and youins.. what a diverse group we are! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 2:41 PM To: jqb7@cdc.gov; histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu; STEGTM@samcstl.org Subject: RE: [Histonet] Texians, OT West Virginian's say warsh, my grandma used to say it and she would also say y'll youngins'. Robyn >>> "Bartlett, Jeanine" 03/30/05 10:41 AM >>> I've lived in the south all of my life and the only person I ever heard say "warsh" was from Minnesota! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therersa Stegall Sent: Wednesday, March 30, 2005 12:39 PM To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: Re: [Histonet] Texians, OT Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From peoshel <@t> wisc.edu Wed Mar 30 14:12:54 2005 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians In-Reply-To: References: Message-ID: Most North Americans don't know what Chile is -- or where. Or did you mean "chili", widely known for destroying gastric mucosa? Mine, anyway -- I prefer to save it for cheese and beer. Phil >Only problem is, Texians don't know what CHILE is... > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >histonet-request@lists.utsouthwestern.edu >Sent: Wednesday, March 30, 2005 10:28 AM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 16, Issue 49 > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From BMolinari <@t> heart.thi.tmc.edu Wed Mar 30 14:13:33 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: Match-achew-chets. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen Broomall Sent: Wednesday, March 30, 2005 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Texians, OT Good grief, how can one mispronounce Massachusetts? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Molinari, Betsy Sent: Wednesday, March 30, 2005 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Texians, OT OK but I have found very few people here in Texas that can pronounce "Massachusetts" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Wednesday, March 30, 2005 12:42 PM To: Therersa Stegall; histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: RE: [Histonet] Texians, OT I've lived in the south all of my life and the only person I ever heard say "warsh" was from Minnesota! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therersa Stegall Sent: Wednesday, March 30, 2005 12:39 PM To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: Re: [Histonet] Texians, OT Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Mar 30 14:01:55 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: I love these tongue-in-cheek comments! However, as we all know, the difference between true Southerners and true Northerners is simply in demeanor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Wednesday, March 30, 2005 2:53 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Texians, OT The Civil War is only over to us northerners. There are plenty in South who are still fighting it again. I imagine the whole exchange has been amusing to the non-US folks. To whom we are *all* Yankees, no matter how many syllables we put in "Mississippi". Phil >Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO >I LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting >emails), I really liked this one. Before all the >Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind >that thumping one's chest about how great a region/state is sounds >great to those who live there but gets old quite quickly to "us >northerners/yankies/etc...". The whole "yankies" thing always did >astound me since it implies lasting unrest over the civil war (which >the south, by the way, lost). Time to drop that one. I'll hold off on >saying how wonderful "the north" is or on the experience I have had >with "the south" since I have no desire to start the Civil War II. One >nation/indivisible sounds good to me. > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: Therersa Stegall [mailto:STEGTM@samcstl.org] >Sent: Wednesday, March 30, 2005 11:39 AM >To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu >Subject: Re: [Histonet] Texians, OT > > >Sounds good to me. I've lived in Texas, and we should let them have >their independence, or return to Mexico, or whatever else the Texans, >Texians, or Texicans want to do. The place is too flat (except for the >rattlesnake infested mountains in the south), and too prone to >tornados. > I can't stand local football fanatics, and cheerleaders, well, yuk. >I'm a Yankee who hasn't lived in the northeast since 1976, but I still >speak understandable english. There is no "r" in wash, and crouch is a >position, not a location. Enough, I'm starting to rant (again). St >Louis isalright, but I'd rather be in the Tetons. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Wed Mar 30 14:16:15 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: There was talk over the last few years of tearing down the Park and building a new ballpark. -----Original Message----- From: Bartlett, Jeanine [mailto:jqb7@cdc.gov] Sent: Wednesday, March 30, 2005 1:39 PM To: Molinari, Betsy Subject: RE: [Histonet] ASCP whine. Please pass the cheese Was Fenway going somewhere? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Wednesday, March 30, 2005 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP whine. Please pass the cheese -----Original Message----- From: Molinari, Betsy Sent: Wednesday, March 30, 2005 1:26 PM To: 'Bell, Lynne' Subject: RE: [Histonet] ASCP whine. Please pass the cheese I'll be at my home too(in Spring Texas, I am a Yankee and a damn Yankee at that!) Latest news is that Fenway is here to stay!!! Now that is something to WUHU about. Betsy MolinariHT (ASCP) Texas Heart Institute Houston,Texas -----Original Message----- From: Bell, Lynne [mailto:Lynne.Bell@hitchcock.org] Sent: Wednesday, March 30, 2005 12:17 PM To: Molinari, Betsy Subject: RE: [Histonet] ASCP whine. Please pass the cheese Home opener, banner raising and ring ceremony - gotta love it! I plan on leaving work early that day to be home to watch all the festivities - couldn't mortgage the house enough to get tickets to be at Fenway............. Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Mar 30 14:10:24 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] ASCP whine. Please pass the cheese Message-ID: No, no, no! It's a "dawg gone minit"! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Wednesday, March 30, 2005 3:14 PM To: histonet@pathology.swmed.edu Subject: Re: [Histonet] ASCP whine. Please pass the cheese Naw whole 'on dare jus a dogue gone minet... nuttin's wrng wid libbin up 'ear in da nord. 40 below kep da riff rraff out, done 'cha no now? Ohh 'ya u bet'cha.. ~ Ford Ford M. Royer, MT(ASCP) Minneapolis, MN ...where 'ya can't take a bath in the winter... water's too hard. Robyn Vazquez wrote: >Juan, >Theres nothing wrong with living up North, you just grow what we refer >to is "web feet" They (feet) help us get around on the streets during >the winter and on the lakes and beaches during the summer. > >Robyn >OHSU > > > >>>>"Joe Nocito" 03/30/05 9:19 AM >>> >>>> >>>> >Juan, >I've lived in Texas longer than I've lived up north. > >Joe > >-----Original Message----- >From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] >Sent: Wednesday, March 30, 2005 8:47 AM >To: Joe Nocito; Histonet >Subject: RE: [Histonet] ASCP whine. Please pass the cheese > > >You know Joe, we Texians were annexed unconstitutionally. We should >just take up arms and re-revolt against the yankee oppressors( I know >you're a transplanted yankee, but we're willing to overlook it). If >Key West can secede why can't we? We were never formally asked to join >the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! >REMEMBER THE ALAMO! > >Juan C. Gutierrez, HT(ASCP) >Histology Laboratory Supervisor >(210)704-2533 > >My opinions are my own and do not reflect those of my employer. Long >live free speech and the Republic of Texas! > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito >Sent: Wednesday, March 30, 2005 8:12 AM >To: Histonet >Subject: [Histonet] ASCP whine. Please pass the cheese > >you might want to hit the delete button on this one. I'm prepared to >get flamed and have my registries revoked, but I have to get something >off my chest. > As some of you may know, ASCP is now in charge of handling the >Pathologists' Assistant exam, of which I am partaking in. Not only did >they raise the exam fee from $150 to $450, we now have to know ICD-9 >codes, not to mention Robbin's Pathology book (which is heavy enough to >break a vase. I know this because it fell out of my hand the other >night and shattered a >vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so >myself. > Moving on, after January 2008, one has to be a graduate of an accredited >program. Well, that's just dandy because we in the South do not have any >programs to attend because they are all up North or on the East Coast. Are >we going to have to repeat the 1860's again? Are we in the South going to >have to resort to revolting and have demonstration marches? Do I sense some >discrimination here? Am I too sensitive? (I think not) > It took us two years here just to get a Histology program up and going at >the local community college. How long is it going to take to start a >master's level program? > There, I've said my piece. thank you for letting me get thing off my chest. >Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll >be sleeping with my dogs. Which is okay, but the fleas are killing me. > One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). > as always, the opinion of this author does not reflect the opinions of the >employees, their children, mothers, lawyers, etc, etc, etc. > > >Joe Nocito, BS, HT(ASCP) QIHC >Histology Manager >Pathology Reference Lab >San Antonio, TX > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Wed Mar 30 14:33:12 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:51 2005 Subject: FW: [Histonet] Texians, OT Message-ID: -----Original Message----- From: Charles.Embrey Sent: Wednesday, March 30, 2005 2:32 PM To: 'Bartlett, Jeanine' Subject: RE: [Histonet] Texians, OT Yeah, when someone disparages the old South demeanor we get. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Wednesday, March 30, 2005 2:02 PM To: Philip Oshel; Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Texians, OT I love these tongue-in-cheek comments! However, as we all know, the difference between true Southerners and true Northerners is simply in demeanor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Wednesday, March 30, 2005 2:53 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Texians, OT The Civil War is only over to us northerners. There are plenty in South who are still fighting it again. I imagine the whole exchange has been amusing to the non-US folks. To whom we are *all* Yankees, no matter how many syllables we put in "Mississippi". Phil >Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO >I LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting >emails), I really liked this one. Before all the >Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind >that thumping one's chest about how great a region/state is sounds >great to those who live there but gets old quite quickly to "us >northerners/yankies/etc...". The whole "yankies" thing always did >astound me since it implies lasting unrest over the civil war (which >the south, by the way, lost). Time to drop that one. I'll hold off on >saying how wonderful "the north" is or on the experience I have had >with "the south" since I have no desire to start the Civil War II. One >nation/indivisible sounds good to me. > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: Therersa Stegall [mailto:STEGTM@samcstl.org] >Sent: Wednesday, March 30, 2005 11:39 AM >To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu >Subject: Re: [Histonet] Texians, OT > > >Sounds good to me. I've lived in Texas, and we should let them have >their independence, or return to Mexico, or whatever else the Texans, >Texians, or Texicans want to do. The place is too flat (except for the >rattlesnake infested mountains in the south), and too prone to >tornados. > I can't stand local football fanatics, and cheerleaders, well, yuk. >I'm a Yankee who hasn't lived in the northeast since 1976, but I still >speak understandable english. There is no "r" in wash, and crouch is a >position, not a location. Enough, I'm starting to rant (again). St >Louis isalright, but I'd rather be in the Tetons. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Mar 30 14:36:34 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: <20050330203635.70681.qmail@web52602.mail.yahoo.com> Where a double digit IQ will take you all the way to the WH. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Janet.Bonner <@t> FLHOSP.ORG Wed Mar 30 14:40:11 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] re: Texas Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41FE@fh2k093.fhmis.net> ALRIGHT, ALREADY!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 12:30 PM To: Larry Woody; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] re: Texas Hey Larry, where you from? I didn't see it on your email. If you look at Florida, anatomically speaking, you see the same result> Remember the Alamo!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Wednesday, March 30, 2005 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: Texas If you look at a map of North America in an anatomical way you get a good view of where Texas stands in the big picture. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbroomal <@t> NEMOURS.ORG Wed Mar 30 14:43:07 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A7975@wlmmsx01.nemours.org> Bless you. Would you like a tissue? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Molinari, Betsy Sent: Wednesday, March 30, 2005 3:14 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Texians, OT Match-achew-chets. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen Broomall Sent: Wednesday, March 30, 2005 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Texians, OT Good grief, how can one mispronounce Massachusetts? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Molinari, Betsy Sent: Wednesday, March 30, 2005 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Texians, OT OK but I have found very few people here in Texas that can pronounce "Massachusetts" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Wednesday, March 30, 2005 12:42 PM To: Therersa Stegall; histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: RE: [Histonet] Texians, OT I've lived in the south all of my life and the only person I ever heard say "warsh" was from Minnesota! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Therersa Stegall Sent: Wednesday, March 30, 2005 12:39 PM To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu Subject: Re: [Histonet] Texians, OT Sounds good to me. I've lived in Texas, and we should let them have their independence, or return to Mexico, or whatever else the Texans, Texians, or Texicans want to do. The place is too flat (except for the rattlesnake infested mountains in the south), and too prone to tornados. I can't stand local football fanatics, and cheerleaders, well, yuk. I'm a Yankee who hasn't lived in the northeast since 1976, but I still speak understandable english. There is no "r" in wash, and crouch is a position, not a location. Enough, I'm starting to rant (again). St Louis isalright, but I'd rather be in the Tetons. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Wed Mar 30 14:44:00 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Texians, OT Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB41FF@fh2k093.fhmis.net> Y'all need to come down to Florida WHERE ALL THE NEW YORKERS (and other assorted Northerners) are!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Wednesday, March 30, 2005 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Texians, OT -----Original Message----- From: Charles.Embrey Sent: Wednesday, March 30, 2005 2:32 PM To: 'Bartlett, Jeanine' Subject: RE: [Histonet] Texians, OT Yeah, when someone disparages the old South demeanor we get. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Wednesday, March 30, 2005 2:02 PM To: Philip Oshel; Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Texians, OT I love these tongue-in-cheek comments! However, as we all know, the difference between true Southerners and true Northerners is simply in demeanor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Wednesday, March 30, 2005 2:53 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Texians, OT The Civil War is only over to us northerners. There are plenty in South who are still fighting it again. I imagine the whole exchange has been amusing to the non-US folks. To whom we are *all* Yankees, no matter how many syllables we put in "Mississippi". Phil >Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO >I LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting >emails), I really liked this one. Before all the >Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind >that thumping one's chest about how great a region/state is sounds >great to those who live there but gets old quite quickly to "us >northerners/yankies/etc...". The whole "yankies" thing always did >astound me since it implies lasting unrest over the civil war (which >the south, by the way, lost). Time to drop that one. I'll hold off on >saying how wonderful "the north" is or on the experience I have had >with "the south" since I have no desire to start the Civil War II. One >nation/indivisible sounds good to me. > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: Therersa Stegall [mailto:STEGTM@samcstl.org] >Sent: Wednesday, March 30, 2005 11:39 AM >To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu >Subject: Re: [Histonet] Texians, OT > > >Sounds good to me. I've lived in Texas, and we should let them have >their independence, or return to Mexico, or whatever else the Texans, >Texians, or Texicans want to do. The place is too flat (except for the >rattlesnake infested mountains in the south), and too prone to >tornados. > I can't stand local football fanatics, and cheerleaders, well, yuk. >I'm a Yankee who hasn't lived in the northeast since 1976, but I still >speak understandable english. There is no "r" in wash, and crouch is a >position, not a location. Enough, I'm starting to rant (again). St >Louis isalright, but I'd rather be in the Tetons. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Wed Mar 30 14:44:18 2005 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Fri Sep 16 15:24:51 2005 Subject: Fwd: RE: [Histonet] re: Texas Message-ID: <20050330204419.96256.qmail@web40904.mail.yahoo.com> Thank you!!!! Note: forwarded message attached. Jill Cox HT (ASCP) --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From krat18 <@t> aol.com Wed Mar 30 14:45:42 2005 From: krat18 <@t> aol.com (krat18@aol.com) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] RE:Charging for immunos In-Reply-To: <20050330170228.81384.qmail@web30402.mail.mud.yahoo.com> References: <20050330170228.81384.qmail@web30402.mail.mud.yahoo.com> Message-ID: <8C70380930B994F-C60-E9AB@mblk-r04.sysops.aol.com> We have one additional question about this issue: in a case where there are several sentinel node blocks on the same case and the docs want to order the same immuno on all these blocks, can you still charge for only ONE immuno, or since they are all from different locations can you charge for them all? Karen_Raterman@ssmhc.com krat18@aol.com From CMCCOLLOUGH <@t> dnr.state.md.us Wed Mar 30 14:51:41 2005 From: CMCCOLLOUGH <@t> dnr.state.md.us (McCollough, Carol) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] was Texians now Merliners Message-ID: Hi Y'all - With all this commentary about the 'south' and the 'north' I thought y'all might appreciate hearing from a border state - the great state of Merlin. We're below the Mason-Dixon Line, but were occupied by the Union during the Civil War, which I believe is over in many parts of Merlin, but not all. Most folks think our capital is Ballamer, but it's really Naplis. That's where George Warshington resigned his commission in the Continental Army. Our nation's capital, Warshington DC, is named for him Go Navy! We still have at least 5 distinct regional dialects within state boundaries, and probably more. Here on the Shore (the Eastern Shore, east of the Chesapeake Bay, God's Country, where there is no life west of, immortalized by former Governor William Donald Schaefer as the S--thouse of Merlin), your county of origin can be determined by the pronunciation of 'sink'; 'zinc' = Dorchester County. Folks from Dorchester and Somerset 'drudge fer arsters', those tasty bivalves. Baltimorese is an entire language unto itself: 'When you go downy oweshun, stay on the payment or you'll get hit by a car' = 'In Ocean City stay on the sidewalk or you'll get hit by a car'. TTFN - Carol ********************** Carol B. McCollough Aquatic Animal Research Pathologist Oyster Disease Research Project Fisheries Service Maryland Department of Natural Resources Cooperative Oxford Laboratory 904 S. Morris Street Oxford, Maryland 21654 410-226-5193 x124 From SAllen <@t> exchange.hsc.mb.ca Wed Mar 30 14:51:22 2005 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Fri Sep 16 15:24:51 2005 Subject: [Histonet] Varicella-Zoster virus Message-ID: <304B5A264EC9974E8121B0EA14A9C3936619F3@hsc01mx1.hsc.mb.ca> Hi, Does anyone have any positive material for Varicella-Zoster virus? I need 10 unstained formalin-fixed, paraffin-embedded slides for immunohistochemistry. I would appreciate any help or suggestions you can provide, although we don't have the facilities to culture the tissue. Debbie, HSC, Winnipeg sallen@hsc.mb.ca This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. From juan.gutierrez <@t> christushealth.org Wed Mar 30 15:14:56 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texians Message-ID: I lived in Albuquerque for a spell, so I know what chile is and miss it terribly. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, March 30, 2005 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Texians Only problem is, Texians don't know what CHILE is... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 30, 2005 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 16, Issue 49 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Opportunity for Histology Laboratory Manager (Raiford, Betty*) 2. Broken glass and Paraffin Block Disposal (Scott, Allison D) 3. RE: ASCP whine. Please pass the cheese (Robyn Vazquez) 4. RE: ASCP whine. Please pass the cheese (GUTIERREZ, JUAN) 5. RE: ASCP whine. Please pass the cheese (Weems, Joyce) 6. Re: ASCP whine. Please pass the cheese (krat18@aol.com) 7. RE: ASCP whine. Please pass the cheese (Rittman, Barry R) 8. RE: ASCP whine. Please pass the cheese (Weems, Joyce) 9. Re: ERK antibody (Joanne Mauger) 10. Texians, OT (Smith, Allen) 11. Leder Stain (Louro, Pedro) 12. CD15 Ab question (Louro, Pedro) 13. Charging for immunos (krat18@aol.com) 14. RE: Texians, OT (GUTIERREZ, JUAN) 15. re: Texas (Larry Woody) 16. FW: [Histonet] Leder Stain (Edmondson David (RBV) NHS Christie Tr) 17. RE:Charging for immunos (Stephen Peters M.D.) 18. RE: re: Texas (GUTIERREZ, JUAN) 19. RE: ASCP whine. Please pass the cheese (Joe Nocito) 20. RE: ASCP whine. Please pass the cheese (Joe Nocito) 21. RE: ASCP whine. Please pass the cheese (Joe Nocito) ---------------------------------------------------------------------- Message: 1 Date: Wed, 30 Mar 2005 09:17:35 -0600 From: "Raiford, Betty*" Subject: [Histonet] Opportunity for Histology Laboratory Manager To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain HISTOLOGY LABORATORY MANAGER Toxicologic Pathology Associates, at the National Center for Toxicological Research in Jefferson, AR is recruiting for a Histology Laboratory Manager. The Histology Laboratory Manager will work directly with the Program Director and the rest of the Pathology Services Project team in our veterinary biomedical research laboratory. The selected candidate must have HT (ASCP certification), at least 5 years of hands-on histologic supervisory experience, familiarity in all aspects of the operation and management of a histology laboratory and staff and knowledge and experience with current technologies. Experience in Good Laboratory Practice (GLP) laboratory environment is highly desirable. BS degree preferred. We offer competitive salary, good benefits package, generous paid time off plan and much more! Interested candidates can submit their resume to: Dr. Paul Mellick, Program Director, Toxicologic Pathology Associates, 3900 NCTR Drive, Jefferson, AR 72079, Phone: (870)543-7022; Fax: (870)543-7401 Email: pmellick@nctr.fda.gov . EOE M/F/DV Betty Raiford Program Manager Toxicologic Pathology Associates 3900 NCTR Rd. Jefferson, AR 72079 phone (870)543-7035 fax (870)543-7401 braiford@nctr.fda.gov CONFIDENTIALITY NOTICE - This email is ONLY for the person(s) named in the message header. Unless otherwise indicated, it contains information that is confidential, privileged or exempt from disclosure under applicable law. If you have received this email in error, please notify the sender of the error and delete the entire message. Thank you. ------------------------------ Message: 2 Date: Wed, 30 Mar 2005 09:57:53 -0600 From: "Scott, Allison D" Subject: [Histonet] Broken glass and Paraffin Block Disposal To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" If anyone has a procedure for the disposal of used glass and paraffin blocks that they are willing to share, I would greatly appreciate it. We are being surveyed by CAP in 2 weeks. This is in regards to ANP.27150 on the AP checklist. Thanks in advance. Allison Scott HT(ASCP) LBJ Hospital 5656 Kelley Houston, Texas 77026 fax: 7135665285 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 3 Date: Wed, 30 Mar 2005 07:59:42 -0800 From: "Robyn Vazquez" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: juan.gutierrez@christushealth.org, histonet@pathology.swmed.edu, JNocito@Pathreflab.com Message-ID: Content-Type: text/plain; charset=us-ascii Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 30 Mar 2005 10:02:50 -0600 From: "GUTIERREZ, JUAN" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "Robyn Vazquez" , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" No it is not. Texians is what the heroes of the Alamo called themselves. It was probably a combination of Texan and Mexican. Yes there was some Mexicans fighting for Texas freedom. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: Wednesday, March 30, 2005 10:00 AM To: GUTIERREZ, JUAN; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 30 Mar 2005 11:04:36 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "Robyn Vazquez" , , , Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA457CF@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 6 Date: Wed, 30 Mar 2005 11:10:14 -0500 From: krat18@aol.com Subject: Re: [Histonet] ASCP whine. Please pass the cheese To: histonet@pathology.swmed.edu Message-ID: <8C7035A17B0CA5F-880-2231A@mblk-r36.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com ------------------------------ Message: 7 Date: Wed, 30 Mar 2005 10:15:05 -0600 From: "Rittman, Barry R" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "histonet" Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0015C05C2@UTHEVS3.mail.uthouston.edu> Content-Type: text/plain; charset="us-ascii" Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 8 Date: Wed, 30 Mar 2005 11:18:55 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "Rittman, Barry R" , "histonet" Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA457D0@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" I'll have to hear it so I can be accepted if I ever go there! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Wednesday, March 30, 2005 11:15 AM To: histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 9 Date: Wed, 30 Mar 2005 11:20:39 -0500 From: "Joanne Mauger" Subject: Re: [Histonet] ERK antibody To: gliuygao@hotmail.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi Yan, I have had success with the Phospho-p44/42 Map Kinase antibody from Cell Signaling Technology-www.cellsignal.com. It stains both Erk1 and Erk2. Order # is 9101 I use it at 1:50 dilution for 1 hour. For antigen retrieval, use pH 10 buffer. Good luck, Jo >>> "yan gao" 03/28/05 1:24 PM >>> Dear Histonet, Does anyone done phospho-ERK antibody? I am working on the RAF pathway need to stain pERK IHC in human tumor paraffin section. Can anyone give some tips? THank a lot. Yan Gao Novartis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 30 Mar 2005 11:24:16 -0500 From: "Smith, Allen" Subject: [Histonet] Texians, OT To: Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3CAC@exchsrv01.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" Col. Travis, the Texian commander at the Alamo, insisted that he was fighting only for the restoration of constitutional government in Mexico (i.e. the Constitution of 1824). After his death, the war became a war for independence. [This is similar to the U.S. and Haitian wars of independence, which started out as wars to secure constitutional rights and became wars of independence only when the colonial power escalated its efforts to deny those rights.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) ------------------------------ Message: 11 Date: Wed, 30 Mar 2005 08:59:51 -0500 From: "Louro, Pedro" Subject: [Histonet] Leder Stain To: histonet@lists.utsouthwestern.edu Message-ID: <4508920F80C0D411B90200508BF9A9F4062B48DE@LAFMSG30.us.schp.com> Content-Type: text/plain I'm looking for information on "Leder Stain" (chloracetate esterase activity)...looking to stain neutrophils on FFPE mouse tissue If someone can supply me with the recipe or the ingredients would be great Thanks Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ Message: 12 Date: Wed, 30 Mar 2005 09:06:33 -0500 From: "Louro, Pedro" Subject: [Histonet] CD15 Ab question To: histonet@lists.utsouthwestern.edu Message-ID: <4508920F80C0D411B90200508BF9A9F4062B48DF@LAFMSG30.us.schp.com> Content-Type: text/plain I'm trying to find a CD15 antibody that will work on FFPE mouse tissue. Looked all over without any success. Would appreciate any information on this matter. Thanks in advance, Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ Message: 13 Date: Wed, 30 Mar 2005 11:36:33 -0500 From: krat18@aol.com Subject: [Histonet] Charging for immunos To: histonet@pathology.swmed.edu Message-ID: <8C7035DC46E333B-594-DD33@mblk-r16.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: krat18@aol.com To: histonet@pathology.swmed.edu Sent: Wed, 30 Mar 2005 11:10:14 -0500 Subject: Re: [Histonet] ASCP whine. Please pass the cheese Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 30 Mar 2005 10:39:24 -0600 From: "GUTIERREZ, JUAN" Subject: RE: [Histonet] Texians, OT To: "Smith, Allen" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Amen brother. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Wednesday, March 30, 2005 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Texians, OT Col. Travis, the Texian commander at the Alamo, insisted that he was fighting only for the restoration of constitutional government in Mexico (i.e. the Constitution of 1824). After his death, the war became a war for independence. [This is similar to the U.S. and Haitian wars of independence, which started out as wars to secure constitutional rights and became wars of independence only when the colonial power escalated its efforts to deny those rights.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 30 Mar 2005 08:58:51 -0800 (PST) From: Larry Woody Subject: [Histonet] re: Texas To: histonet@lists.utsouthwestern.edu Message-ID: <20050330165851.78704.qmail@web52610.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii If you look at a map of North America in an anatomical way you get a good view of where Texas stands in the big picture. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! ------------------------------ Message: 16 Date: Wed, 30 Mar 2005 17:59:08 +0100 From: "Edmondson David \(RBV\) NHS Christie Tr" Subject: FW: [Histonet] Leder Stain To: "Histonet \(E-mail 2\)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Have no idea whose name should be attributed to this but.. Slides to water. We have 30mg of Fast Blue BB base and 6mg of alpha Naphthyl Chloroacetate The first dissolved in 0.2M pH 7.6 TrisMalate buffer, the second in a few drops of Dimethyl Formamide and then added to the buffer. All is mixed and the slides immersed for 20-30mins. 37deg C is fine but go for a shorter time to avoid background. Counterstain say Neutral Red, rinse then dehydrate rapidly and mount. Take too long in alcohol or waiting to be mounted may abolish the staining. Check hazards. Do risk assessment To save calculations, could look in Bancroft and Stevens appendix for buffer table.where dissolve 2.42g TRIS + 2.32g Maleic acid in 100ml of water then 0.8g NaOH in 100ml of water 25ml of first plus 29ml of alkali give pH7.6 Dave Christie Hosp Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Louro, Pedro Sent: 30 March 2005 15:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leder Stain I'm looking for information on "Leder Stain" (chloracetate esterase activity)...looking to stain neutrophils on FFPE mouse tissue If someone can supply me with the recipe or the ingredients would be great Thanks Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 30 Mar 2005 09:02:28 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] RE:Charging for immunos To: Histonet@lists.utsouthwestern.edu Message-ID: <20050330170228.81384.qmail@web30402.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi karen, My understanding of this reg is that you can only bill once for each specimen part. For example if you have a case such in multiple parts (i.e. a colon cancer with the colon as part one, a liver biopsy as part two and an abdominal wall met as part three) if you order S 100 on blocks A, B and C of part one you can only bill for one. But if you order an s 100 on a colon slide from part one and and s 100 on the liver biopsy from part two you can bill for each. I belive the same is true for special stains like AFB and fungal stains. ect. If anyone has different info I would appreciate your interpretation. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 ------------------------------ Message: 18 Date: Wed, 30 Mar 2005 11:06:50 -0600 From: "GUTIERREZ, JUAN" Subject: RE: [Histonet] re: Texas To: "Larry Woody" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" That's right we got all the b.... DON'T MESS WITH TEXAS! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Wednesday, March 30, 2005 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: Texas If you look at a map of North America in an anatomical way you get a good view of where Texas stands in the big picture. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 30 Mar 2005 11:19:28 -0600 From: "Joe Nocito" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "GUTIERREZ, JUAN" , "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Juan, I've lived in Texas longer than I've lived up north. Joe -----Original Message----- From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] Sent: Wednesday, March 30, 2005 8:47 AM To: Joe Nocito; Histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 30 Mar 2005 11:22:15 -0600 From: "Joe Nocito" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Karen, I don't think it is a CAP requirement, I think it has something to do with Medicare/ Medicaid and the insurance companies for reimbursement. Hold on, I have to check my ICD-9 code book Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of krat18@aol.com Sent: Wednesday, March 30, 2005 10:10 AM To: histonet@pathology.swmed.edu Subject: Re: [Histonet] ASCP whine. Please pass the cheese Didn't I read that CAP has a regulation that a lab cannot charge for more than one of each immuno on the same case, e.g., cannot charge for two S-100's on the same case, even though they're on different blocks? Does anyone know where to find the justification for that? Karen_Raterman@ssmhc.com krat18@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 30 Mar 2005 11:22:45 -0600 From: "Joe Nocito" Subject: RE: [Histonet] ASCP whine. Please pass the cheese To: "Rittman, Barry R" , "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Thank you Barry Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rittman, Barry R Sent: Wednesday, March 30, 2005 10:15 AM To: histonet Subject: RE: [Histonet] ASCP whine. Please pass the cheese Joyce Texian and Texicans are words used here, just like y'all and y'all y'all. These words have arisen because many of the northerners are unable to understand the culture here and be accepted. Once you understand how to pronounce these words and actually use them everyday then you understand something about the culture here and you are accepted. I can say this as I migrated from the north (Iowa) 15 years ago and, like Joe, have loved every minute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, March 30, 2005 10:05 AM To: Robyn Vazquez; juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese It just became one - hasn't had time to make it to publication yet, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Wednesday, March 30, 2005 11:00 AM To: juan.gutierrez@christushealth.org; histonet@pathology.swmed.edu; JNocito@Pathreflab.com Subject: RE: [Histonet] ASCP whine. Please pass the cheese Juan, Is Texians a new word? For the life of me can't find it in any dictionary up North here!!! Robyn Vazquez OHSU >>> "GUTIERREZ, JUAN" 03/30/05 6:46 AM >>> You know Joe, we Texians were annexed unconstitutionally. We should just take up arms and re-revolt against the yankee oppressors( I know you're a transplanted yankee, but we're willing to overlook it). If Key West can secede why can't we? We were never formally asked to join the United States of Aggression. LONG LIVE THE REPUBLIC OF TEXAS! REMEMBER THE ALAMO! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech and the Republic of Texas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 30, 2005 8:12 AM To: Histonet Subject: [Histonet] ASCP whine. Please pass the cheese you might want to hit the delete button on this one. I'm prepared to get flamed and have my registries revoked, but I have to get something off my chest. As some of you may know, ASCP is now in charge of handling the Pathologists' Assistant exam, of which I am partaking in. Not only did they raise the exam fee from $150 to $450, we now have to know ICD-9 codes, not to mention Robbin's Pathology book (which is heavy enough to break a vase. I know this because it fell out of my hand the other night and shattered a vase.) Have you seen the ICD-9 book? Rather extension reading if I do say so myself. Moving on, after January 2008, one has to be a graduate of an accredited program. Well, that's just dandy because we in the South do not have any programs to attend because they are all up North or on the East Coast. Are we going to have to repeat the 1860's again? Are we in the South going to have to resort to revolting and have demonstration marches? Do I sense some discrimination here? Am I too sensitive? (I think not) It took us two years here just to get a Histology program up and going at the local community college. How long is it going to take to start a master's level program? There, I've said my piece. thank you for letting me get thing off my chest. Oh yeah, I'm collecting money to replace the vase I broke. Until then, I'll be sleeping with my dogs. Which is okay, but the fleas are killing me. One other thing, I like Swiss, Muenster, Colby, Cheddar (sharp). as always, the opinion of this author does not reflect the opinions of the employees, their children, mothers, lawyers, etc, etc, etc. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. 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Saint Josephs Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 16, Issue 49 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Wed Mar 30 15:24:37 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] was Texians now Merliners In-Reply-To: Message-ID: I may not get to heaven, but Texas is as close as I'll get -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of McCollough, Carol Sent: Wednesday, March 30, 2005 2:52 PM To: Histonet (E-mail 2) Subject: [Histonet] was Texians now Merliners Hi Y'all - With all this commentary about the 'south' and the 'north' I thought y'all might appreciate hearing from a border state - the great state of Merlin. We're below the Mason-Dixon Line, but were occupied by the Union during the Civil War, which I believe is over in many parts of Merlin, but not all. Most folks think our capital is Ballamer, but it's really Naplis. That's where George Warshington resigned his commission in the Continental Army. Our nation's capital, Warshington DC, is named for him Go Navy! We still have at least 5 distinct regional dialects within state boundaries, and probably more. Here on the Shore (the Eastern Shore, east of the Chesapeake Bay, God's Country, where there is no life west of, immortalized by former Governor William Donald Schaefer as the S--thouse of Merlin), your county of origin can be determined by the pronunciation of 'sink'; 'zinc' = Dorchester County. Folks from Dorchester and Somerset 'drudge fer arsters', those tasty bivalves. Baltimorese is an entire language unto itself: 'When you go downy oweshun, stay on the payment or you'll get hit by a car' = 'In Ocean City stay on the sidewalk or you'll get hit by a car'. TTFN - Carol ********************** Carol B. McCollough Aquatic Animal Research Pathologist Oyster Disease Research Project Fisheries Service Maryland Department of Natural Resources Cooperative Oxford Laboratory 904 S. Morris Street Oxford, Maryland 21654 410-226-5193 x124 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Wed Mar 30 15:27:22 2005 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texians, OT In-Reply-To: References: Message-ID: <4.3.2.7.2.20050330142306.00ca3b20@algranth.inbox.email.arizona.edu> We just had our yearly re-enactment of the farthest west Civil War battle which was fought at Picacho Peak, somewhere between Phoenix and Tucson on I-10. There are some who think they are still fighting the war. Andi At 01:53 PM 3/30/2005 -0600, Philip Oshel wrote: >The Civil War is only over to us northerners. There are plenty in South >who are still fighting it again. >I imagine the whole exchange has been amusing to the non-US folks. To whom >we are *all* Yankees, no matter how many syllables we put in "Mississippi". > >Phil > >>Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO I >>LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting >>emails), I really liked this one. Before all the >>Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind that >>thumping one's chest about how great a region/state is sounds great to those >>who live there but gets old quite quickly to "us >>northerners/yankies/etc...". The whole "yankies" thing always did astound >>me since it implies lasting unrest over the civil war (which the south, by >>the way, lost). Time to drop that one. I'll hold off on saying how >>wonderful "the north" is or on the experience I have had with "the south" >>since I have no desire to start the Civil War II. One nation/indivisible >>sounds good to me. >> >>Glen Dawson BS, HT & QIHC (ASCP) >>IHC Manager >>Milwaukee, WI >> >>-----Original Message----- >>From: Therersa Stegall [mailto:STEGTM@samcstl.org] >>Sent: Wednesday, March 30, 2005 11:39 AM >>To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu >>Subject: Re: [Histonet] Texians, OT >> >> >>Sounds good to me. I've lived in Texas, and we should let them have >>their independence, or return to Mexico, or whatever else the Texans, >>Texians, or Texicans want to do. The place is too flat (except for the >>rattlesnake infested mountains in the south), and too prone to tornados. >> I can't stand local football fanatics, and cheerleaders, well, yuk. >>I'm a Yankee who hasn't lived in the northeast since 1976, but I still >>speak understandable english. There is no "r" in wash, and crouch is a >>position, not a location. Enough, I'm starting to rant (again). St >>Louis isalright, but I'd rather be in the Tetons. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >-- >Philip Oshel >Supervisor, BBPIC microscopy facility >Department of Animal Sciences >University of Wisconsin >1675 Observatory Drive >Madison, WI 53706 >voice: (608) 263-4162 >fax: (608) 262-5157 (dept. fax) > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From vazquezr <@t> ohsu.edu Wed Mar 30 15:43:32 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texians Message-ID: Oh now hold on, us northerns do know where it is...it is in Alaska where it is realllly cold. Robyn >>> Philip Oshel 03/30/05 12:12 PM >>> Most North Americans don't know what Chile is -- or where. Or did you mean "chili", widely known for destroying gastric mucosa? Mine, anyway -- I prefer to save it for cheese and beer. Phil >Only problem is, Texians don't know what CHILE is... > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >histonet-request@lists.utsouthwestern.edu >Sent: Wednesday, March 30, 2005 10:28 AM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 16, Issue 49 > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Wed Mar 30 15:49:07 2005 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texians, OT In-Reply-To: Message-ID: <01LMIC6GPT5E8WXXXE@Macon2.Mercer.edu> And I was thinking it was geography. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Wednesday, March 30, 2005 3:02 PM To: Philip Oshel; Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Texians, OT I love these tongue-in-cheek comments! However, as we all know, the difference between true Southerners and true Northerners is simply in demeanor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Wednesday, March 30, 2005 2:53 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Texians, OT The Civil War is only over to us northerners. There are plenty in South who are still fighting it again. I imagine the whole exchange has been amusing to the non-US folks. To whom we are *all* Yankees, no matter how many syllables we put in "Mississippi". Phil >Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO >I LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting >emails), I really liked this one. Before all the >Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind >that thumping one's chest about how great a region/state is sounds >great to those who live there but gets old quite quickly to "us >northerners/yankies/etc...". The whole "yankies" thing always did >astound me since it implies lasting unrest over the civil war (which >the south, by the way, lost). Time to drop that one. I'll hold off on >saying how wonderful "the north" is or on the experience I have had >with "the south" since I have no desire to start the Civil War II. One >nation/indivisible sounds good to me. > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: Therersa Stegall [mailto:STEGTM@samcstl.org] >Sent: Wednesday, March 30, 2005 11:39 AM >To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu >Subject: Re: [Histonet] Texians, OT > > >Sounds good to me. I've lived in Texas, and we should let them have >their independence, or return to Mexico, or whatever else the Texans, >Texians, or Texicans want to do. The place is too flat (except for the >rattlesnake infested mountains in the south), and too prone to >tornados. > I can't stand local football fanatics, and cheerleaders, well, yuk. >I'm a Yankee who hasn't lived in the northeast since 1976, but I still >speak understandable english. There is no "r" in wash, and crouch is a >position, not a location. Enough, I'm starting to rant (again). St >Louis isalright, but I'd rather be in the Tetons. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgrow <@t> bmnet.com Wed Mar 30 16:26:00 2005 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Re: Broken glass and Paraffin Block Disposal In-Reply-To: <200503301720.j2UHKcJc000517@dns1.bmnet.com> Message-ID: SUBJECT: USED SLIDES AND BLOCKS DISPOSAL INTRODUCTION The laboratory must dispose of its waste in a responsible manner, consistent with hospital policy, municipal, state and federal regulations. Therefore, to protect the safety of others and to meet regulatory requirements, laboratory waste disposal must be accomplished with care. PROCEDURE 1. Used glass slides: a. Are to be disposed of as regulated medical waste. b. Dispose of used glass slides from the histology laboratory into approved red sharps containers. c. Do not overfill container. d. Close securely. e. Call environmental services for pick-up. f. Environmental Services will follow hospital policy for disposal of regulated medical waste. 2. Used paraffin blocks: a. The Director of Laboratories must approve of any blocks for disposal. g. Blocks are to be disposed of as regulated medical waste. b. Dispose of blocks in approved sturdy biomedical waste container, double lined with red bags. c. Do not exceed 30 pounds weight per box. d. Close securely. e. Label Clearly: ? Pathology Waste for Incineration Only.? f. Call environmental services for pick-up. g. Environmental Services will follow hospital policy for incineration of regulated medical waste. Adjust the verbage to comply with your hospital/pathologist/CAP requirements. Good luck. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 2 Date: Wed, 30 Mar 2005 09:57:53 -0600 From: "Scott, Allison D" Subject: [Histonet] Broken glass and Paraffin Block Disposal To: "'histonet@lists.utsouthwestern.edu'" If anyone has a procedure for the disposal of used glass and paraffin blocks that they are willing to share, I would greatly appreciate it. We are being surveyed by CAP in 2 weeks. This is in regards to ANP.27150 on the AP checklist. Thanks in advance. Allison Scott HT(ASCP) LBJ Hospital 5656 Kelley Houston, Texas 77026 fax: 7135665285 From jfish <@t> gladstone.ucsf.edu Wed Mar 30 16:30:43 2005 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Frozen Spleen w/bubbles In-Reply-To: <1112124746.4249ad4aaabcb@imp.vet.upenn.edu> References: <1112124746.4249ad4aaabcb@imp.vet.upenn.edu> Message-ID: Hello histonetters, I am cryosectioning unfixed fresh frozen spleens and when they are sectioned I can see a billion bubbles under the section. Can anyone explain- 1. What is happening? 2. How can I avoid them? 3. Is there a fixation procedure that can help? The sections are falling off of the slide during staining. Thank you for your help, Jo Dee ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-5766 Fax: (415) 355-0230 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 From timothy.macatee <@t> med.nyu.edu Wed Mar 30 16:36:23 2005 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texas our texas Message-ID: Now for my two cents. After spending twelve years in Houston and two years in Dallas, I don't miss much. If Houston isn't hell it's not a fer' piece. Who ever said, "Let's live here!" back in the 1700's must moved in in winter. The interesting thing about Houston is everyone there is from somewhere else, mostly the north east. As a matter of fact I met my wife in Houston even though we were born in the same hospital in Philadelphia. Don't even get me started on Dallas. I like it here in the North east, except for the toll roads, the prices, the commute, the lack of good Mexican food the... -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From kbroomal <@t> NEMOURS.ORG Wed Mar 30 16:43:14 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Frozen Spleen w/bubbles Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A797C@wlmmsx01.nemours.org> I'm sure someone more knowledgeable will come along soon, but could it be freezing artifact? How are you freezing the samples? Kristen Broomall -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jo Dee Fish Sent: Wednesday, March 30, 2005 5:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Spleen w/bubbles Hello histonetters, I am cryosectioning unfixed fresh frozen spleens and when they are sectioned I can see a billion bubbles under the section. Can anyone explain- 1. What is happening? 2. How can I avoid them? 3. Is there a fixation procedure that can help? The sections are falling off of the slide during staining. Thank you for your help, Jo Dee ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-5766 Fax: (415) 355-0230 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfish <@t> gladstone.ucsf.edu Wed Mar 30 16:50:30 2005 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Frozen Spleen w/bubbles In-Reply-To: <9BCAC308B27CAD4196D8AC9ADF037AAA110A797C@wlmmsx01.nemours.org> References: <9BCAC308B27CAD4196D8AC9ADF037AAA110A797C@wlmmsx01.nemours.org> Message-ID: Hi Kristen, Actually, the tissue looks okay, the bubbles look like they are between the section and the glass. There are lots and lots of tiny bubbles... Jo Dee At 5:43 PM -0500 3/30/05, Kristen Broomall wrote: >I'm sure someone more knowledgeable will come along soon, but could it be >freezing artifact? How are you freezing the samples? > >Kristen Broomall ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-5766 Fax: (415) 355-0230 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 From Barry.R.Rittman <@t> uth.tmc.edu Wed Mar 30 16:51:03 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texas our texas Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0015C07D8@UTHEVS3.mail.uthouston.edu> As we all seem to be for or against Tejas, I should point out that it is not where you live so much as how you live. I have been happy in England (although I did complain about the weather), happy in Iowa (although I did complain about the weather) and happy in Houston (although I still complain about the weather). If I could I would have a mixture of all three places, the food in Houston, the educational resources in Iowa and the history (and English usage)in England. It seems to me that while the people in all those places were a lot different from each other they were all basically nice when given the chance. I think that to get along in any place you have to accept its positive and negatives. It is fine to complain as it has been shown that those that complain at least care, however lets complain about the big things we are able to change. Hope my wife doesn't see this as she will say I should complain less especially about the traffic here. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy Macatee Sent: Wednesday, March 30, 2005 4:36 PM To: Histonet@Pathology.swmed.edu Subject: [Histonet] Texas our texas Now for my two cents. After spending twelve years in Houston and two years in Dallas, I don't miss much. If Houston isn't hell it's not a fer' piece. Who ever said, "Let's live here!" back in the 1700's must moved in in winter. The interesting thing about Houston is everyone there is from somewhere else, mostly the north east. As a matter of fact I met my wife in Houston even though we were born in the same hospital in Philadelphia. Don't even get me started on Dallas. I like it here in the North east, except for the toll roads, the prices, the commute, the lack of good Mexican food the... -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Wed Mar 30 17:04:42 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Frozen Spleen w/bubbles Message-ID: Jo Dee, Are you using a cryogun? That could be a cultprit. Also, are putting the slides directly into your fixative? What kind of stainer are you using? If your using a chain, put it in the heated section for 5 to 30 seconds. I used to have this problem and when we put it in the heated section for just a few seconds>30sec that solved the problem. OR you could have a bad lot of slides. Robyn OHSU >>> Jo Dee Fish 03/30/05 2:30 PM >>> Hello histonetters, I am cryosectioning unfixed fresh frozen spleens and when they are sectioned I can see a billion bubbles under the section. Can anyone explain- 1. What is happening? 2. How can I avoid them? 3. Is there a fixation procedure that can help? The sections are falling off of the slide during staining. Thank you for your help, Jo Dee ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-5766 Fax: (415) 355-0230 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Wed Mar 30 19:16:13 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] RE:Frozen Spleen w/bubbles Message-ID: <20050331011613.55158.qmail@web30407.mail.mud.yahoo.com> Could you be looking at bubbles in the coverslipping medium. For any reason did the medium get agitated? Such as in combining the contents of an almost empty bottle. From Joanholtz <@t> aol.com Wed Mar 30 22:23:36 2005 From: Joanholtz <@t> aol.com (Joanholtz@aol.com) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] gravity for levity Message-ID: <11.424a5bf0.2f7cd548@aol.com> Greetings to all, Why is it that when the ASCP is mentioned in the subject matter, questions die quickly on the vine? Speaking of the ASCP is like talking with the Wizard of Oz; you know, "ignore the man behind the curtain." The ASCP is the governing body of our livelihood. How they wield the power of their monopoly has effects on each and every one of us. Joe has raised questions many times about whether they make decisions before contemplating their effects. I don't think it is a minor oversight that they would place criteria for certification that defies the geography of available resources. Previous posts have indicated serious inconsistencies with the certification process. There seems to be no verifiable insight into the actual workings of this organization. I find this lack of transparency and accountability troubling. The ASCP must have something more to offer than a trial by fire. It bothers me as well as others that we don't know how they make their decisions and now it has gone farther as to why they make these decisions. I would like to see our profession grow and get the respect it deserves but I think that would be by helping each and every one of us; allowing all the boats to rise in the tide. Regards, Joan From ree3 <@t> leicester.ac.uk Thu Mar 31 02:40:20 2005 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texas our texas Message-ID: The UNITED States of America eh what !? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Timothy Macatee Sent: 30 March 2005 23:36 To: Histonet@Pathology.swmed.edu Subject: [Histonet] Texas our texas Now for my two cents. After spending twelve years in Houston and two years in Dallas, I don't miss much. If Houston isn't hell it's not a fer' piece. Who ever said, "Let's live here!" back in the 1700's must moved in in winter. The interesting thing about Houston is everyone there is from somewhere else, mostly the north east. As a matter of fact I met my wife in Houston even though we were born in the same hospital in Philadelphia. Don't even get me started on Dallas. I like it here in the North east, except for the toll roads, the prices, the commute, the lack of good Mexican food the... -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Sheppard <@t> Health-Partners.org Thu Mar 31 05:44:15 2005 From: John.Sheppard <@t> Health-Partners.org (John.Sheppard@Health-Partners.org) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] New ASCP charge for HT's Message-ID: Has anyone else noticed that ASCP now charges a $75 practical exam fee. I believe this to be on top of the $125 application/examination fee for the written test. Just thought I would put it out there, for anyone that cares. Anyone else want to leave ASCP and charter the Confederated Histology and Pathology Associates of America? John Sheppard HT (ASCP) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mucram11 <@t> comcast.net Thu Mar 31 05:50:51 2005 From: mucram11 <@t> comcast.net (pam marcum) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] gravity for levity References: <11.424a5bf0.2f7cd548@aol.com> Message-ID: <424BE41B.000001.03312@YOUR-4DI1S53IME> AMEN -------Original Message------- From: Joanholtz@aol.com Date: 03/30/05 23:25:42 To: histonet@lists.utsouthwestern.edu Cc: shapegirl26@hotmail.com Subject: RE: [Histonet] gravity for levity Greetings to all, Why is it that when the ASCP is mentioned in the subject matter, questions die quickly on the vine? Speaking of the ASCP is like talking with the Wizard of Oz; you know, "ignore the man behind the curtain." The ASCP is the governing body of our livelihood. How they wield the power of their monopoly has effects on each and every one of us. Joe has raised questions many times about whether they make decisions before contemplating their effects. I don't think it is a minor oversight that they would place criteria for certification that defies the geography of available resources. Previous posts have indicated serious inconsistencies with the certification process. There seems to be no verifiable insight into the actual workings of this organization. I find this lack of transparency and accountability troubling. The ASCP must have something more to offer than a trial by fire. It bothers me as well as others that we don't know how they make their decisions and now it has gone farther as to why they make these decisions. I would like to see our profession grow and get the respect it deserves but I think that would be by helping each and every one of us; allowing all the boats to rise in the tide. Regards, Joan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jengirl1014 <@t> yahoo.com Thu Mar 31 07:07:13 2005 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] RE: was Texians now Merlins Message-ID: <20050331130713.62181.qmail@web60606.mail.yahoo.com> You forgot to mention how here in Ballamer we reed books in da lieberry sos we can becomes educated. We also stay outta da way of da po-leece and always, always root for de Oryuls! We now root for de Ravens, too! Dis here is a die hard sports are and we's are dag gone proud of it! Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From gradice <@t> richmond.edu Thu Mar 31 08:23:44 2005 From: gradice <@t> richmond.edu (Gary Radice) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] protozoan stains at neutral pH Message-ID: I have a colleague who is studying a calcified protozoan. She is specifically interested in calcification, and she is looking for stains she can use that are not acidic and that don't require acid differentiation steps, so that she doesn't inadvertently decalcify the specimens. Any thoughts about nuclear or counterstains that might work in this situation? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice From JNocito <@t> Pathreflab.com Thu Mar 31 08:33:56 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] gravity for levity In-Reply-To: <11.424a5bf0.2f7cd548@aol.com> Message-ID: Thanks Joan for the support. What we need is another governing board to compete with ASCP, but I know that I am wearing ruby slippers and walking with the tin man, scarecrow and lion because I know that it will never happen. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joanholtz@aol.com Sent: Wednesday, March 30, 2005 10:24 PM To: histonet@lists.utsouthwestern.edu Cc: shapegirl26@hotmail.com Subject: RE: [Histonet] gravity for levity Greetings to all, Why is it that when the ASCP is mentioned in the subject matter, questions die quickly on the vine? Speaking of the ASCP is like talking with the Wizard of Oz; you know, "ignore the man behind the curtain." The ASCP is the governing body of our livelihood. How they wield the power of their monopoly has effects on each and every one of us. Joe has raised questions many times about whether they make decisions before contemplating their effects. I don't think it is a minor oversight that they would place criteria for certification that defies the geography of available resources. Previous posts have indicated serious inconsistencies with the certification process. There seems to be no verifiable insight into the actual workings of this organization. I find this lack of transparency and accountability troubling. The ASCP must have something more to offer than a trial by fire. It bothers me as well as others that we don't know how they make their decisions and now it has gone farther as to why they make these decisions. I would like to see our profession grow and get the respect it deserves but I think that would be by helping each and every one of us; allowing all the boats to rise in the tide. Regards, Joan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Thu Mar 31 08:34:36 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] New ASCP charge for HT's In-Reply-To: Message-ID: I'm there Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of John.Sheppard@Health-Partners.org Sent: Thursday, March 31, 2005 5:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New ASCP charge for HT's Has anyone else noticed that ASCP now charges a $75 practical exam fee. I believe this to be on top of the $125 application/examination fee for the written test. Just thought I would put it out there, for anyone that cares. Anyone else want to leave ASCP and charter the Confederated Histology and Pathology Associates of America? John Sheppard HT (ASCP) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Thu Mar 31 08:08:37 2005 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texians Message-ID: It's that Hendrix classic. Voodoo Chile >>> Philip Oshel 03/30/05 03:12PM >>> Most North Americans don't know what Chile is -- or where. Or did you mean "chili", widely known for destroying gastric mucosa? Mine, anyway -- I prefer to save it for cheese and beer. Phil >Only problem is, Texians don't know what CHILE is... > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >histonet-request@lists.utsouthwestern.edu >Sent: Wednesday, March 30, 2005 10:28 AM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 16, Issue 49 > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Mar 31 08:35:27 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] gravity for levity Message-ID: Joan, My dealings with the ASCP are much like my dealings with police officers, postal carriers, and people cooking my food. Complaints to the latter 3 can result in tickets, late bill payments, and large amounts of spit in your food (if you are lucky). Complaints with the first can result in non-certification or non-renewal of certifications/qualifications so it is best to sacrifice a chicken at the ASCP alter and keep your head down since their approval/disapproval appears to be about as arbitrary as the direction of the wind. Todays lesson: don't mess with people/entities that can mess with you on a grand scale. Glen Dawson -----Original Message----- From: Joanholtz@aol.com [mailto:Joanholtz@aol.com] Sent: Wednesday, March 30, 2005 10:24 PM To: histonet@lists.utsouthwestern.edu Cc: shapegirl26@hotmail.com Subject: RE: [Histonet] gravity for levity Greetings to all, Why is it that when the ASCP is mentioned in the subject matter, questions die quickly on the vine? Speaking of the ASCP is like talking with the Wizard of Oz; you know, "ignore the man behind the curtain." The ASCP is the governing body of our livelihood. How they wield the power of their monopoly has effects on each and every one of us. Joe has raised questions many times about whether they make decisions before contemplating their effects. I don't think it is a minor oversight that they would place criteria for certification that defies the geography of available resources. Previous posts have indicated serious inconsistencies with the certification process. There seems to be no verifiable insight into the actual workings of this organization. I find this lack of transparency and accountability troubling. The ASCP must have something more to offer than a trial by fire. It bothers me as well as others that we don't know how they make their decisions and now it has gone farther as to why they make these decisions. I would like to see our profession grow and get the respect it deserves but I think that would be by helping each and every one of us; allowing all the boats to rise in the tide. Regards, Joan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Thu Mar 31 08:48:50 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] request for data Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0015C0852@UTHEVS3.mail.uthouston.edu> I am trying to get some information from y'all regarding work loads. In the past couple of years we have seen numerous discussions on Histonet re number of blocks and numbers of histotechs preparing these. What have often been missing are other factors such as.... Are there any laboratory attendants? Who does the grossing? What record keeping is necessary? What administrative support is available, e.g. who send out the mailing containers etc.? What proportions of the total numbers require decalcification other than for small bone biopsies? How is tissue processed, type of machine (old Technicon, versus newer VIP as an example)? How is tissue stained, by this I refer to manual versus machine.? If machine staining, are there separate machines for routine stains, special stains and IHC? Is this lab also responsible for preparing slides for research investigations? This is of course not really a survey and I do not expect direct responses for this. What I would very much appreciate is if anyone can direct me to any documented resources that deal with this. My next question will be simpler and will deal with "The meaning of Life" Thanks Barry Telephone: 713-500-4134 From gradice <@t> richmond.edu Thu Mar 31 08:47:11 2005 From: gradice <@t> richmond.edu (Gary Radice) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] neutral stains for protozoa Message-ID: I have a colleague who is studying a calcified protozoan. She is specifically interested in calcification, and she is looking for stains she can use that are not acidic and that don't require acid differentiation steps, so that she doesn't inadvertently decalcify the specimens. Any thoughts about nuclear or counterstains that might work in this situation? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice From juan.gutierrez <@t> christushealth.org Thu Mar 31 08:55:32 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] New ASCP charge for HT's Message-ID: The first shot has been fired. C'mon y'all lets give 'em a hell of a fite. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John.Sheppard@Health-Partners.org Sent: Thursday, March 31, 2005 5:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New ASCP charge for HT's Has anyone else noticed that ASCP now charges a $75 practical exam fee. I believe this to be on top of the $125 application/examination fee for the written test. Just thought I would put it out there, for anyone that cares. Anyone else want to leave ASCP and charter the Confederated Histology and Pathology Associates of America? John Sheppard HT (ASCP) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Thu Mar 31 09:01:44 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] New ASCP charge for HT's Message-ID: <20050331150144.4812.qmail@web52604.mail.yahoo.com> Count me in also, I've been paying my dues for 25 yrs. and the ASCP has done absolutely nothing to advance the profession. Larry --------------------------------- Do you Yahoo!? Make Yahoo! your home page From kelly.mcqueeney <@t> bms.com Thu Mar 31 09:10:57 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Storage after perfusion of brain tissue, what is the best method? Message-ID: <424C1301.10304@bms.com> Dear Histonetters, I have perfused rat brain with 4% paraformaldehyde, placed in fix O/N and then added a 10% and 30% sucrose solution. What is the next step toward long-term storage of this tissue if I plan on using a cryostat to section? How would you recommend storing this tissue and what is the next step after sucrose. I will not be sectioning for a few weeks. Also, how do I store the slides after sectioning? Do I let them dry for a few hours like I do with fresh tissue? Thanks for your help, Kelly McQueeney From amy_vanko <@t> merck.com Thu Mar 31 09:26:53 2005 From: amy_vanko <@t> merck.com (Vanko, Amy) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] RE: Storage after perfusion of brain tissue, what is the best met hod? Message-ID: Hi Kelly, You should store them in the minus 20 freezer. I don't think that sucrose can cryoprotect much colder than that. As far as saving sections, I have only done free-floating immuno on perfused sections so we saved our sections in a cryoprotectant solution in the minus 20 freezer. This was for stereology, so we actually saved one section/well in 96 well plates. Amy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D Mcqueeney Sent: Thursday, March 31, 2005 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage after perfusion of brain tissue, what is the best method? Dear Histonetters, I have perfused rat brain with 4% paraformaldehyde, placed in fix O/N and then added a 10% and 30% sucrose solution. What is the next step toward long-term storage of this tissue if I plan on using a cryostat to section? How would you recommend storing this tissue and what is the next step after sucrose. I will not be sectioning for a few weeks. Also, how do I store the slides after sectioning? Do I let them dry for a few hours like I do with fresh tissue? Thanks for your help, Kelly McQueeney _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From pex0220 <@t> yahoo.com.cn Thu Mar 31 09:33:39 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Immunofluorescence Message-ID: <20050331153339.40988.qmail@web15502.mail.cnb.yahoo.com> Hello,all, I am doing immunofluorescence in bone sections, but the results are not good, I do not know the reasons, can anybody do me a favor? My questions: 1. In bone sections, I found that some positions are stained, but some positions are not stained, I do not know why it appears. 2. In addition, this protein should be expressed strongly in bone marrow, but sometimes it is very weak, sometimes it is not stained. why? Thank you! Guofeng --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From mprice26 <@t> juno.com Thu Mar 31 09:43:57 2005 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] RE: PA Programs Message-ID: <20050331.074358.25501.157442@webmail04.nyc.untd.com> How can I get a list of all the PA Programs/Schools in the United States? Thank you. Marsha Price From leswes <@t> shaw.ca Thu Mar 31 09:55:29 2005 From: leswes <@t> shaw.ca (Lesley Weston) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texians, OT In-Reply-To: <07AB60D5D7B9754EBF56F360F98D083DEB41FF@fh2k093.fhmis.net> Message-ID: If you can find them among all the Canadians there! -- Lesley Weston > From: "Bonner, Janet" > Date: Wed, 30 Mar 2005 15:44:00 -0500 > To: "'Charles.Embrey'" , > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Texians, OT > > Y'all need to come down to Florida WHERE ALL THE NEW YORKERS (and other > assorted Northerners) are!! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > Charles.Embrey > Sent: Wednesday, March 30, 2005 3:33 PM > To: histonet@lists.utsouthwestern.edu > Subject: FW: [Histonet] Texians, OT > > > > > -----Original Message----- > From: Charles.Embrey > Sent: Wednesday, March 30, 2005 2:32 PM > To: 'Bartlett, Jeanine' > Subject: RE: [Histonet] Texians, OT > > Yeah, when someone disparages the old South demeanor we get. > > Chuck > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, Jeanine > Sent: Wednesday, March 30, 2005 2:02 PM > To: Philip Oshel; Histonet@Pathology.swmed.edu > Subject: RE: [Histonet] Texians, OT > > I love these tongue-in-cheek comments! However, as we all know, the > difference between true Southerners and true Northerners is simply in > demeanor. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip > Oshel > Sent: Wednesday, March 30, 2005 2:53 PM > To: Histonet@Pathology.swmed.edu > Subject: RE: [Histonet] Texians, OT > > > The Civil War is only over to us northerners. There are plenty in > South who are still fighting it again. > I imagine the whole exchange has been amusing to the non-US folks. To > whom we are *all* Yankees, no matter how many syllables we put in > "Mississippi". > > Phil > >> Although I'm not sure what all this WAHOOOO I LIVE IN TEXAS or WAHOOOO >> I LIVE IN "THE SOUTH" is all about (I really need to stop mass deleting > >> emails), I really liked this one. Before all the >> Texans/Texians/Texicans/Rebs hop all over Ms. Stegall, keep in mind >> that thumping one's chest about how great a region/state is sounds >> great to those who live there but gets old quite quickly to "us >> northerners/yankies/etc...". The whole "yankies" thing always did >> astound me since it implies lasting unrest over the civil war (which >> the south, by the way, lost). Time to drop that one. I'll hold off on >> saying how wonderful "the north" is or on the experience I have had >> with "the south" since I have no desire to start the Civil War II. One >> nation/indivisible sounds good to me. >> >> Glen Dawson BS, HT & QIHC (ASCP) >> IHC Manager >> Milwaukee, WI >> >> -----Original Message----- >> From: Therersa Stegall [mailto:STEGTM@samcstl.org] >> Sent: Wednesday, March 30, 2005 11:39 AM >> To: histonet@lists.utsouthwestern.edu; asmith@mail.barry.edu >> Subject: Re: [Histonet] Texians, OT >> >> >> Sounds good to me. I've lived in Texas, and we should let them have >> their independence, or return to Mexico, or whatever else the Texans, >> Texians, or Texicans want to do. The place is too flat (except for the > >> rattlesnake infested mountains in the south), and too prone to >> tornados. >> I can't stand local football fanatics, and cheerleaders, well, yuk. >> I'm a Yankee who hasn't lived in the northeast since 1976, but I still >> speak understandable english. There is no "r" in wash, and crouch is a >> position, not a location. Enough, I'm starting to rant (again). St >> Louis isalright, but I'd rather be in the Tetons. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > Philip Oshel > Supervisor, BBPIC microscopy facility > Department of Animal Sciences > University of Wisconsin > 1675 Observatory Drive > Madison, WI 53706 > voice: (608) 263-4162 > fax: (608) 262-5157 (dept. fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Thu Mar 31 10:10:27 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] RE: PA Programs Message-ID: http://www.pathologistsassistants.org/Default.aspx?m=1012&s=145 Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mprice26@juno.com Sent: Thursday, March 31, 2005 9:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: PA Programs How can I get a list of all the PA Programs/Schools in the United States? Thank you. Marsha Price _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Thu Mar 31 10:17:54 2005 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Storage after perfusion of brain tissue, what is the best method? Message-ID: <5784D843593D874C93E9BADCB87342AB44F7CD@tpiserver03.Coretech-holdings.com> They are fixed after overnight exposure. 30% sucrose is the last step. They can be stored indefinitely. They may be somewhat overfixed for some antibody studies, fine for anything else. Store in sucrose on a shelf. Yes, after sectioning, dry the slides, at least a few hours, overnight is ok. Store for some time that way, but stain and coverslip for permanent storage. Be sure the tissue is frozen as fast as possible, immersion in isopentane at -80C is best, on a cold pedestal and covered with powdered dry ice is second. Freezing in the crystat on a pedastal is unacceptable, will get swiss cheese freezing artifact damage in the tissue. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D Mcqueeney Sent: Thursday, March 31, 2005 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage after perfusion of brain tissue,what is the best method? Dear Histonetters, I have perfused rat brain with 4% paraformaldehyde, placed in fix O/N and then added a 10% and 30% sucrose solution. What is the next step toward long-term storage of this tissue if I plan on using a cryostat to section? How would you recommend storing this tissue and what is the next step after sucrose. I will not be sectioning for a few weeks. Also, how do I store the slides after sectioning? Do I let them dry for a few hours like I do with fresh tissue? Thanks for your help, Kelly McQueeney _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhawkins <@t> UTMB.EDU Thu Mar 31 10:28:02 2005 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Texians and chili Message-ID: <8D6F233E2A5D574B929F3944F3316FD01A5921@EXCH2K3.utmb.edu> Surprisingly, Texas chili isn't quite as old as the independent nation of Texas. Here are a couple of links on the history and construction of chili and the origin of the word. Chili peppers, of course, were immortalized by the Red Hot Chili Peppers band. http://www.texascooking.com/features/oct2000raven.htm http://www.bigbruce.com/chiliinfo.cfm?refid=4 http://www.redhotchilipeppers.com/ Hal Hawkins, UT Medical Branch, Galveston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Thursday, March 31, 2005 8:09 AM To: Histonet@Pathology.swmed.edu; peoshel@wisc.edu Subject: Re: [Histonet] Texians It's that Hendrix classic. Voodoo Chile >>> Philip Oshel 03/30/05 03:12PM >>> Most North Americans don't know what Chile is -- or where. Or did you mean "chili", widely known for destroying gastric mucosa? Mine, anyway -- I prefer to save it for cheese and beer. Phil >Only problem is, Texians don't know what CHILE is... > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >histonet-request@lists.utsouthwestern.edu >Sent: Wednesday, March 30, 2005 10:28 AM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 16, Issue 49 > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Mar 31 10:27:49 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] New ASCP charge for HT's Message-ID: I'm all for succeeding from that union if we can still get jobs. Glen Dawson -----Original Message----- From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] Sent: Thursday, March 31, 2005 8:56 AM To: John.Sheppard@Health-Partners.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New ASCP charge for HT's The first shot has been fired. C'mon y'all lets give 'em a hell of a fite. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John.Sheppard@Health-Partners.org Sent: Thursday, March 31, 2005 5:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New ASCP charge for HT's Has anyone else noticed that ASCP now charges a $75 practical exam fee. I believe this to be on top of the $125 application/examination fee for the written test. Just thought I would put it out there, for anyone that cares. Anyone else want to leave ASCP and charter the Confederated Histology and Pathology Associates of America? John Sheppard HT (ASCP) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eearle <@t> abrazohealth.com Thu Mar 31 11:03:35 2005 From: eearle <@t> abrazohealth.com (Earle, Elizabeth) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] CD2 Message-ID: <4727B24D9A8575479222635B57E3B3DBCB1EB8@mail.vhswest.local> Does anyone know of a CD2 that works in FFPE tissue? Thanks E Earle From failm <@t> musc.edu Thu Mar 31 11:15:30 2005 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] neutral stains for protozoa Message-ID: I use an aqueous 1% light green to counterstain in an Alizarin Red S Rena Fail >> Gary Radice 03/31/05 09:47AM >>> I have a colleague who is studying a calcified protozoan. She is specifically interested in calcification, and she is looking for stains she can use that are not acidic and that don't require acid differentiation steps, so that she doesn't inadvertently decalcify the specimens. Any thoughts about nuclear or counterstains that might work in this situation? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJones <@t> cvm.tamu.edu Thu Mar 31 11:20:43 2005 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Tissue Cassettes Message-ID: Hi Nita, Surgipath has mesh cassettes that don't trap the air. I just recently saw a sample. I would recommend them. Sarah Sarah Jones, HT(ASCP) Histology Lab Dept. of Veterinary Integrative Biosciences College of Veterinary Medicine Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> "Nita Searcy" 03/28/05 3:20 PM >>> Are there any new "biopsy" cassettes o the market? Know of multi-chambered with tiny holes- as well as "biopsy" cassettes. Have had some "lost" "small" specimens lately and chief pathologist over histology does not want to use papers or bags. Any help would be appreciated. Nita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Debbiejsiena <@t> aol.com Thu Mar 31 11:35:26 2005 From: Debbiejsiena <@t> aol.com (Debbiejsiena@aol.com) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Wanted Histotechnician Message-ID: Tissue Techniques Pathology Labs in Dallas, Texas has two (2) positions open. The first position is a lead technician, this person must be able to lead by example, must know special stains and immuno's they must be able to communicate both verbally and written, must be able to complete all paperwork to keep up with CLIA regulations. The second position is a general tech, this person must be able to keep up with daily pace, must be able to embed, cut and stain. Both positions will report to the Owner/ President (Debbie Siena). To be considered for either position please submit your qualifications by Fax: 972-241-4747, e-mail: tissuetech@juno.com or mail: Tissue Techniques 13016 Bee Street Suite # 100 Dallas, Texas 75234-6158. Phone: 972-241-6277. Thanks for your time. Sincerely: Debbie From John.Sheppard <@t> Health-Partners.org Thu Mar 31 11:36:38 2005 From: John.Sheppard <@t> Health-Partners.org (John.Sheppard@Health-Partners.org) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] RE: Tissue Cassetes Message-ID: We have used the microbiopsy cassettes from Surgipath for skin shave biopsies for the past year and they work great! I would recomend them strongly if you can afford them. John Sheppard HT(ASCP) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Janet.Bonner <@t> FLHOSP.ORG Thu Mar 31 11:50:59 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] New ASCP charge for HT's Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4203@fh2k093.fhmis.net> HAS ANYONE SENT THEM THE ORIGINAL eMAIL!!?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: John.Sheppard@Health-Partners.org; histonet@lists.utsouthwestern.edu Sent: 3/31/2005 9:55 AM Subject: RE: [Histonet] New ASCP charge for HT's The first shot has been fired. C'mon y'all lets give 'em a hell of a fite. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John.Sheppard@Health-Partners.org Sent: Thursday, March 31, 2005 5:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New ASCP charge for HT's Has anyone else noticed that ASCP now charges a $75 practical exam fee. I believe this to be on top of the $125 application/examination fee for the written test. Just thought I would put it out there, for anyone that cares. Anyone else want to leave ASCP and charter the Confederated Histology and Pathology Associates of America? John Sheppard HT (ASCP) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Thu Mar 31 11:55:22 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] CD2 In-Reply-To: <4727B24D9A8575479222635B57E3B3DBCB1EB8@mail.vhswest.local> Message-ID: Elizabeth, We use a CD2 clone MT910 on formalin fixed paraffin embedded (FFPE) tissue. It is a bit tricky. We use a long heat retrieval with 10mM citrate, pH6.0. We microwave pressure cook with this antibody, having the slides under pressure for 15 minutes. Also, the antibody requires a tyramide amplification detection system for FFPE. We use the CSAII from Dakocytomation. Hope this helps. Good luck with it. Patti Loykasek PhenoPath Laboratories Seattle, WA> Does anyone know of a CD2 that works in FFPE tissue? > > Thanks > > E Earle > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Mar 31 12:14:06 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] CD2 Message-ID: Novacastra CD2 works on FFPE tissues (Cat. # NCL-CD2-271). Vector is the US distributer. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Earle, Elizabeth [mailto:eearle@abrazohealth.com] Sent: Thursday, March 31, 2005 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD2 Does anyone know of a CD2 that works in FFPE tissue? Thanks E Earle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Salvacion.DeLovino <@t> cshs.org Thu Mar 31 12:31:23 2005 From: Salvacion.DeLovino <@t> cshs.org (DeLovino, Salvacion S.) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] GMS Message-ID: Is your deionized water still good? We had the same problem one time and the culprit was the water. Salve ------------------------------ >From: "Karen Harmon" >To: >Subj>From: "Karen Harmon" >To: >Subject: [Histonet] Pneumocystis carnii staining >Date: Mon, 28 Mar 2005 16:25:33 -0600 > >We use a GMS stain to demonstrate pneuomcyctis with successful results >about 50% of the time. We have taken this procedure apart step by step, >solution by solution and are at our wits end to determine the cause for >failure. I can assure you that over the past 3 weeks, there is no variable >that we have not tried. The ironic part is we never have a problem >demonstrating fungi with the GMS, in fact, we never had any problems at all >with other silver procedures. > >Can anyone help with our hit and miss problem with pneumocystis? > ------------------------------ IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From settembr <@t> umdnj.edu Thu Mar 31 12:33:59 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] CD2 Message-ID: Hello Elizabeth, I use CD2 on FFPE human tissue. It's from DakoCytomation cat.# M0720. I use it with a tonsil control and retrieve in a steamer for 40 minutes in Dako's TRS solution. My dilution is 1:100 for 15 minute incubation and the detection kit I use is Dako's Envision + mouse. Works nicely. Dana Settembre University Hospital - UMDNJ >>> "Earle, Elizabeth" 3/31/2005 12:03:35 PM >>> Does anyone know of a CD2 that works in FFPE tissue? Thanks E Earle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Thu Mar 31 12:52:06 2005 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR)) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] GMS Message-ID: <8F3AB322628548428A992EFB0E80F5D315D630AC@nihexchange8.nih.gov> I always use Ultra Pure water for all my Silver Staining. Getting beautiful results. Armed Forces Institute of Pathology, Neuropathology gave me this hint. Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Pain and Neurosensory Mechanism Branch Drug Discovery > ---------- > From: DeLovino, Salvacion S. > Sent: Thursday, March 31, 2005 12:31 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] GMS > > Is your deionized water still good? We had the same problem one time and > the > culprit was the water. > > Salve > > > > > ------------------------------ > > >From: "Karen Harmon" > >To: > >Subj>From: "Karen Harmon" > >To: > >Subject: [Histonet] Pneumocystis carnii staining > >Date: Mon, 28 Mar 2005 16:25:33 -0600 > > > >We use a GMS stain to demonstrate pneuomcyctis with successful results > >about 50% of the time. We have taken this procedure apart step by step, > > >solution by solution and are at our wits end to determine the cause for > >failure. I can assure you that over the past 3 weeks, there is no > variable > > >that we have not tried. The ironic part is we never have a problem > >demonstrating fungi with the GMS, in fact, we never had any problems at > all > > >with other silver procedures. > > > >Can anyone help with our hit and miss problem with pneumocystis? > > > ------------------------------ > > > > > IMPORTANT WARNING: This message is intended for the use of the person or > entity to which it is addressed and may contain information that is > privileged and confidential, the disclosure of which is governed by > applicable law. If the reader of this message is not the intended > recipient, or the employee or agent responsible for delivering it to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this information is STRICTLY PROHIBITED. > > If you have received this message in error, please notify us immediately > by calling (310) 423-6428 and destroy the related message. Thank You for > your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gcallis <@t> montana.edu Thu Mar 31 13:04:08 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Re:Frozen Spleen w/bubbles In-Reply-To: References: <1112124746.4249ad4aaabcb@imp.vet.upenn.edu> Message-ID: <6.0.0.22.1.20050331115056.01b70438@gemini.msu.montana.edu> Jo Dee, I have questions first. What species is the spleen from? How thick are the sections? What temperature are you sectioning at? i.e. knife temperature? Block/tissue temperature? How to you pick up a FS onto the slide? Slide to section so it comes onto glass surface, another method? What kind of blades are you using? Are you sections perfectly flat under antiroll device or by brush method, whichever you use? What kind of slides are you using? Plus Charge? Before IHC do you air dry your sections at RT or overnight before fixation with what???? How are you fixing your spleens? Do you use a peroxidase blocker that has a high concentration of hydrogen peroxide? At 03:30 PM 3/30/2005, you wrote: >I am cryosectioning unfixed fresh frozen spleens and when they are >sectioned I can see a billion bubbles under the section. Can anyone explain- >1. What is happening? >2. How can I avoid them? >3. Is there a fixation procedure that can help? >The sections are falling off of the slide during staining. >Thank you for your help, >Jo Dee >********************************************************************** >********** > >Jo Dee Fish >Research Technologist III >Gladstone Institute of Cardiovascular Disease Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Thu Mar 31 13:08:55 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Mounting media bubbles RE:Frozen Spleen w/bubbles In-Reply-To: <20050331011613.55158.qmail@web30407.mail.mud.yahoo.com> References: <20050331011613.55158.qmail@web30407.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20050331120709.01b6e480@gemini.msu.montana.edu> When I have coverslipping bubbles on stained frozen sections of spleen, the mounting media bubbles are on TOP of the section and not under the sections. At 06:16 PM 3/30/2005, you wrote: >Could you be looking at bubbles in the coverslipping medium. For any >reason did the medium get agitated? Such as in combining the contents of >an almost empty bottle. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From JNocito <@t> Pathreflab.com Thu Mar 31 13:14:32 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] New ASCP charge for HT's In-Reply-To: <07AB60D5D7B9754EBF56F360F98D083DEB4203@fh2k093.fhmis.net> Message-ID: I know someone has to be listening because my comment about them raising the PA fee from $150 to $450 appeared to be answered by some one on the inside. Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonner, Janet Sent: Thursday, March 31, 2005 11:51 AM To: 'GUTIERREZ, JUAN '; 'histonet-bounces@lists.utsouthwestern.edu '; 'John.Sheppard@Health-Partners.org '; 'histonet@lists.utsouthwestern.edu ' Subject: RE: [Histonet] New ASCP charge for HT's HAS ANYONE SENT THEM THE ORIGINAL eMAIL!!?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: John.Sheppard@Health-Partners.org; histonet@lists.utsouthwestern.edu Sent: 3/31/2005 9:55 AM Subject: RE: [Histonet] New ASCP charge for HT's The first shot has been fired. C'mon y'all lets give 'em a hell of a fite. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John.Sheppard@Health-Partners.org Sent: Thursday, March 31, 2005 5:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New ASCP charge for HT's Has anyone else noticed that ASCP now charges a $75 practical exam fee. I believe this to be on top of the $125 application/examination fee for the written test. Just thought I would put it out there, for anyone that cares. Anyone else want to leave ASCP and charter the Confederated Histology and Pathology Associates of America? John Sheppard HT (ASCP) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Thu Mar 31 13:18:40 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] gravity for levity In-Reply-To: Message-ID: Sorry Glen, I have to disagree, it gets to a point when someone has to stand up to this totalitarian state. I'll do it, but I need a guarantee that I'll have some support. They can mess with as much as they want, but it wouldn't be wise to get this Italian backed into a corner. So, how do y'all think my anger and stress management classes are going? Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawson, Glen Sent: Thursday, March 31, 2005 8:35 AM To: Joanholtz@aol.com; histonet@lists.utsouthwestern.edu Cc: shapegirl26@hotmail.com Subject: RE: [Histonet] gravity for levity Joan, My dealings with the ASCP are much like my dealings with police officers, postal carriers, and people cooking my food. Complaints to the latter 3 can result in tickets, late bill payments, and large amounts of spit in your food (if you are lucky). Complaints with the first can result in non-certification or non-renewal of certifications/qualifications so it is best to sacrifice a chicken at the ASCP alter and keep your head down since their approval/disapproval appears to be about as arbitrary as the direction of the wind. Todays lesson: don't mess with people/entities that can mess with you on a grand scale. Glen Dawson -----Original Message----- From: Joanholtz@aol.com [mailto:Joanholtz@aol.com] Sent: Wednesday, March 30, 2005 10:24 PM To: histonet@lists.utsouthwestern.edu Cc: shapegirl26@hotmail.com Subject: RE: [Histonet] gravity for levity Greetings to all, Why is it that when the ASCP is mentioned in the subject matter, questions die quickly on the vine? Speaking of the ASCP is like talking with the Wizard of Oz; you know, "ignore the man behind the curtain." The ASCP is the governing body of our livelihood. How they wield the power of their monopoly has effects on each and every one of us. Joe has raised questions many times about whether they make decisions before contemplating their effects. I don't think it is a minor oversight that they would place criteria for certification that defies the geography of available resources. Previous posts have indicated serious inconsistencies with the certification process. There seems to be no verifiable insight into the actual workings of this organization. I find this lack of transparency and accountability troubling. The ASCP must have something more to offer than a trial by fire. It bothers me as well as others that we don't know how they make their decisions and now it has gone farther as to why they make these decisions. I would like to see our profession grow and get the respect it deserves but I think that would be by helping each and every one of us; allowing all the boats to rise in the tide. Regards, Joan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Thu Mar 31 13:21:27 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] GMS In-Reply-To: Message-ID: Probably the water, we have dionized tanks and sometimes we have problems from tank to tank. it took a while to figure this out. If you can, purchase dionized water from a vendor. I know this is costly, but if you use it just for GMS stains, you have to figure the cost of repeats, tech time, delays in completing cases. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of DeLovino, Salvacion S. Sent: Thursday, March 31, 2005 12:31 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS Is your deionized water still good? We had the same problem one time and the culprit was the water. Salve ------------------------------ >From: "Karen Harmon" >To: >Subj>From: "Karen Harmon" >To: >Subject: [Histonet] Pneumocystis carnii staining >Date: Mon, 28 Mar 2005 16:25:33 -0600 > >We use a GMS stain to demonstrate pneuomcyctis with successful results >about 50% of the time. We have taken this procedure apart step by step, >solution by solution and are at our wits end to determine the cause for >failure. I can assure you that over the past 3 weeks, there is no variable >that we have not tried. The ironic part is we never have a problem >demonstrating fungi with the GMS, in fact, we never had any problems at all >with other silver procedures. > >Can anyone help with our hit and miss problem with pneumocystis? > ------------------------------ IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Thu Mar 31 13:27:23 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] PTAH Mordant Message-ID: Hey y'all, since we no longer have Zenkers because of the mercury, what is being used as a mordant for the PTAH stain? My pathologists have been hounding me all week, but with my other net postings and replies, I just forgot. Thanks Histoland Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX From jfish <@t> gladstone.ucsf.edu Thu Mar 31 13:50:48 2005 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Re:Frozen Spleen w/bubbles In-Reply-To: <6.0.0.22.1.20050331115056.01b70438@gemini.msu.montana.edu> References: <1112124746.4249ad4aaabcb@imp.vet.upenn.edu> <6.0.0.22.1.20050331115056.01b70438@gemini.msu.montana.edu> Message-ID: >Dear Gayle, Thank you for your response, here are some answers to your questions. In addition, the bubbles appear right after the section is picked up, before any other process is performed, such as staining, IHC, fixation, or coverslipping. I am at a loss. I don't see this problem with any of my other tissues. > >What species is the spleen from? >Mouse >How thick are the sections? >10um >What temperature are you sectioning at? i.e. knife temperature? >Block/tissue temperature? >-20C >How to you pick up a FS onto the slide? Slide to section so it >comes onto glass surface, another method? >Glass to section. >What kind of blades are you using? >ThermoShandon MB35 Premier >Are you sections perfectly flat under antiroll device or by brush >method, whichever you use? >Yes, nice and flat, I tried both methods on two different cryostats. >What kind of slides are you using? Plus Charge? >Colorfrost Plus slides. >Before IHC do you air dry your sections at RT or overnight before >fixation with what???? >I'm not doing the IHC, the investigator is. But anyway, I see the >bubbles before anything is done to the slide, before fixation, >drying, etc. >How are you fixing your spleens? >She has tried 3% paraformaldehyde, cold acetone, and zinc formalin. >Do you use a peroxidase blocker that has a high concentration of >hydrogen peroxide? >I don't know what she is doing. The sections came off of the slide >regardless, whether or not IHC was performed. ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-5766 Fax: (415) 355-0230 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 From victor <@t> pathology.washington.edu Thu Mar 31 14:01:55 2005 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] gravity for levity In-Reply-To: References: Message-ID: <424C5733.8070602@pathology.washington.edu> Joe, You can have my support. In the early days of my Histology career I was proud to pay my dues and get my stickers, looked pretty impressive on the wall. All this time I never attended any sort of ASCP meeting because they offerred nothing that was related to my working position. When I realized that paying my dues had nothing to do with maintaining my HT(ASCP) and I think they stopped sending me stickers also, I stopped renewing. I don't recall how many years it has been but I still get a renewal form from time to time. Maybe they would take notice if everyone boycotted and didn't renew their membership. I lived in San Antonio for 5 months in the early 70's while stationed at Fort Sam Houston. I wasn't crazy about the weather, but enjoyed the town and people. Victor Joe Nocito wrote: >Sorry Glen, I have to disagree, > it gets to a point when someone has to stand up to this totalitarian state. >I'll do it, but I need a guarantee that I'll have some support. They can >mess with as much as they want, but it wouldn't be wise to get this Italian >backed into a corner. > So, how do y'all think my anger and stress management classes are going? > >Joe > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawson, >Glen >Sent: Thursday, March 31, 2005 8:35 AM >To: Joanholtz@aol.com; histonet@lists.utsouthwestern.edu >Cc: shapegirl26@hotmail.com >Subject: RE: [Histonet] gravity for levity > > >Joan, > >My dealings with the ASCP are much like my dealings with police officers, >postal carriers, and people cooking my food. Complaints to the latter 3 can >result in tickets, late bill payments, and large amounts of spit in your >food (if you are lucky). Complaints with the first can result in >non-certification or non-renewal of certifications/qualifications so it is >best to sacrifice a chicken at the ASCP alter and keep your head down since >their approval/disapproval appears to be about as arbitrary as the direction >of the wind. Todays lesson: don't mess with people/entities that can mess >with you on a grand scale. > >Glen Dawson > >-----Original Message----- >From: Joanholtz@aol.com [mailto:Joanholtz@aol.com] >Sent: Wednesday, March 30, 2005 10:24 PM >To: histonet@lists.utsouthwestern.edu >Cc: shapegirl26@hotmail.com >Subject: RE: [Histonet] gravity for levity > > >Greetings to all, > >Why is it that when the ASCP is mentioned in the subject matter, questions >die quickly on the vine? Speaking of the ASCP is like talking with the >Wizard >of Oz; you know, "ignore the man behind the curtain." > >The ASCP is the governing body of our livelihood. How they wield the power >of their monopoly has effects on each and every one of us. Joe has raised >questions many times about whether they make decisions before contemplating >their >effects. I don't think it is a minor oversight that they would place >criteria >for certification that defies the geography of available resources. > >Previous posts have indicated serious inconsistencies with the certification > >process. There seems to be no verifiable insight into the actual workings >of >this organization. I find this lack of transparency and accountability >troubling. The ASCP must have something more to offer than a trial by fire. >It >bothers me as well as others that we don't know how they make their >decisions and >now it has gone farther as to why they make these decisions. I would like >to >see our profession grow and get the respect it deserves but I think that >would be by helping each and every one of us; allowing all the boats to rise >in >the tide. > >Regards, >Joan >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From sv18 <@t> columbia.edu Thu Mar 31 14:05:50 2005 From: sv18 <@t> columbia.edu (sv18@columbia.edu) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Re: Histonet Digest, Vol 16, Issue 48/ orecin In-Reply-To: <200503301452.j2UEpxEn000537@tepin.cc.columbia.edu> References: <200503301452.j2UEpxEn000537@tepin.cc.columbia.edu> Message-ID: <1112299550.424c581e9db8d@cubmail.cc.columbia.edu> > You'll find the procedure on Page 198 of Theory and Practice of Histotechnology, Sheehan and Hrapchak. However, here we use Victoria Blue, it will stain the elastin plus hepatitis B surface antigen, Hemosidderin Bile pigment and copper-associated protein if done correctly. > > > Hi, > > I was asked to do Orcein stain for elastic fibres. I couldn't > find method. Could anybody tell me where I can find it or send me > a copy? I really apperciate any input. > > From slappycraw <@t> yahoo.com Thu Mar 31 14:11:32 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] National Histology Walkout Message-ID: <20050331201132.35979.qmail@web52602.mail.yahoo.com> A few years ago I was at a training seminar at Dako Corp in carpenteria , Ca. and there were Techs there from all over the U.S. A. and I remember one guy from New York saying that if Histotechs across the country all walked out for one week that then maybe things would change as far as respect and wages, ect. who knows it would be very hard to organize but what an impact it would have. Larry __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From DRG <@t> Stowers-Institute.org Thu Mar 31 14:10:54 2005 From: DRG <@t> Stowers-Institute.org (Grant, Debra) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Glass Coverslippers Message-ID: <1099822661-743080970@pathology.swmed.edu> Hi All, We would like to purchase a glass coverslipper very soon, and are interested in positive and negative feedback on all glass coverslippers. Thanks for your time in advance! P.S Is anyone demoing the Leica Coverslipper at the moment? Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org (913)926-4305 From Pantur <@t> aol.com Thu Mar 31 14:19:17 2005 From: Pantur <@t> aol.com (Pantur@aol.com) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Wanted: Old Technicon Tissue Processors, model 2A Message-ID: <7e.66942201.2f7db545@aol.com> We are currently looking for Technicon Tissue Processors, model 2A, monos or duos, working or for parts Best regards, Angelos Panagopoulos Manager Pantur, Inc. 5126 East 5th Street..........P O Box 6471 Austin, TX 78702...............Austin, TX 78762 USA.................................USA Telephone: (512) 385-6232 Fax: (512) 385-6253 e-mail: pantur@aol.com From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Mar 31 14:23:23 2005 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Glass Coverslippers Message-ID: <3D502BBF5356D31184650090275B750D0346C957@MAIL> Debby, We will be demoing the Leica Coverslipper next week. I'll be glad to let you know how it goes. Does anyone have any experience with the Sakura Glas coverslipper? We're trying that one in a few weeks. Thanks. Stacy McLaughlin -----Original Message----- From: Grant, Debra [mailto:DRG@Stowers-Institute.org] Sent: Thursday, March 31, 2005 3:11 PM To: Histonet Subject: [Histonet] Glass Coverslippers Hi All, We would like to purchase a glass coverslipper very soon, and are interested in positive and negative feedback on all glass coverslippers. Thanks for your time in advance! P.S Is anyone demoing the Leica Coverslipper at the moment? Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org (913)926-4305 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. From jqb7 <@t> cdc.gov Thu Mar 31 13:59:52 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] PTAH Mordant Message-ID: We use the Zenker's that has zinc chloride instead of mercury. Works freat and do "de-zenkerization" required! We get ours from Newcomer Supply. Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, March 31, 2005 2:27 PM To: Histonet Subject: [Histonet] PTAH Mordant Hey y'all, since we no longer have Zenkers because of the mercury, what is being used as a mordant for the PTAH stain? My pathologists have been hounding me all week, but with my other net postings and replies, I just forgot. Thanks Histoland Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WWmn916 <@t> aol.com Thu Mar 31 14:56:22 2005 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] National Histology Walkout Message-ID: <1f3.6a9b1fd.2f7dbdf6@aol.com> Wow, I must be living in a bubble in the Sacramento area. I personally don't feel victimized or taken advantage of in this profession. Is it just that I've been lucky for the past 11 years to experience respect as a histotech? I, for one, would NOT join a national walk-out for that reason. It's belief in patient care, the science and the art that I love so much about histology. I know doctors in this area respect their techs. Sincerely, Deborah King, HT(ASCP) Histology Supervisor Sacramento, CA From maria <@t> ski.org Thu Mar 31 14:58:51 2005 From: maria <@t> ski.org (Maria Mejia) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Re:Frozen Spleen w/bubbles References: <1112124746.4249ad4aaabcb@imp.vet.upenn.edu> <6.0.0.22.1.20050331115056.01b70438@gemini.msu.montana.edu> Message-ID: <424C648B.3040003@ski.org> Hello, Jo Dee, I feel for you! I wish I could be there to see first hand this strange (type) of bubbles on freshly cut cryo tissue sections. I'd like ask how the tissue (spleen) was initially frozen? How does the frozen spleen surface look to you as you're cutting into it (before) picking up any sections? Does the surface of the tissue look normal - by that I mean does it look smooth? You've probably have already surmised the reason for the tissue falling off - layer /number of bubbles of air between the tissue and the glass surface of the slide. Maria Bartola Mejia Smith-Kettlewell Eye Research Institute San Francisco, CA 94103 Email: maria@ski.org Phone: (415)-345-2185 Jo Dee Fish wrote: >> Dear Gayle, > > > Thank you for your response, here are some answers to your questions. > In addition, the bubbles appear right after the section is picked up, > before any other process is performed, such as staining, IHC, > fixation, or coverslipping. I am at a loss. I don't see this problem > with any of my other tissues. > >> >> What species is the spleen from? >> Mouse >> How thick are the sections? >> 10um >> What temperature are you sectioning at? i.e. knife temperature? >> Block/tissue temperature? >> -20C >> How to you pick up a FS onto the slide? Slide to section so it comes >> onto glass surface, another method? >> Glass to section. >> What kind of blades are you using? >> ThermoShandon MB35 Premier >> Are you sections perfectly flat under antiroll device or by brush >> method, whichever you use? >> Yes, nice and flat, I tried both methods on two different cryostats. >> What kind of slides are you using? Plus Charge? >> Colorfrost Plus slides. >> Before IHC do you air dry your sections at RT or overnight before >> fixation with what???? >> I'm not doing the IHC, the investigator is. But anyway, I see the >> bubbles before anything is done to the slide, before fixation, >> drying, etc. >> How are you fixing your spleens? >> She has tried 3% paraformaldehyde, cold acetone, and zinc formalin. >> Do you use a peroxidase blocker that has a high concentration of >> hydrogen peroxide? >> I don't know what she is doing. The sections came off of the slide >> regardless, whether or not IHC was performed. > > > ********************************************************************** > ********** > > Jo Dee Fish > Research Technologist III > Gladstone Institute of Cardiovascular Disease > > Telephone: (415) 734-5766 > Fax: (415) 355-0230 > E-mail: jfish@gladstone.ucsf.edu > > Mailing address: > The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Thu Mar 31 15:06:41 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] National Histology Walkout Message-ID: <20050331210641.27944.qmail@web52603.mail.yahoo.com> I too have been lucky enough to work at some places that treated me with respect, ie; the Mayo Clinic, and my current job at Amgen, but the field is under paid and a lot of places you don't get respect or treated fairly. Someone delivering packages for UPS makes more than the average Tech, is that right. Larry --------------------------------- Do you Yahoo!? Yahoo! Mail - You care about security. So do we. From Luis.Chiriboga <@t> med.nyu.edu Thu Mar 31 15:42:26 2005 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] NYSHS 2005 Meeting Message-ID: The New York State Society for Histotechnology Annual meeting will be held May 6th and 7th at the Holiday Inn in Binghamton New York. Lodging: Hotel reservations may be made by contacting the Holiday Inn in Binghamton http://www.vistahotels.com/hotels_binghamton.htm Telephone: 1-607-722-1212 Fax:1-607-722-6063 Email:hiarena@stny.rr.com Be sure to request the special NYSHS rates. Exhibitor/Vendor Information: Please e-mail Linda Chen at KOOK55@HOTMAIL.COM Membership Information: Please e-mail Linda Chen at KOOK55@HOTMAIL.COM Registration Information: To obtain a program directly or to request a registration form please contact Judy LaDuc at jaladuc@capital.net ____________________________________________________________________________ MEETING ITINERARY EXHIBIT HOURS: Friday May 6th ,5:00-7:00 (Wine & Cheese Reception ) Saturday May 7, 8:00-3:00 Exhibit Hours ON SITE MEETING REGISTRATION Friday May 6, 11:30-2:30 Saturday May 7, 7:00-8:00AM Friday May 6, 2005 Afternoon 12:30-1:30 Gross & Microscopic Techniques of Forensic Anthropology: Case Studies Dr. Dawnie Steadman Forensic anthropology is the application of skeletal biological principles to medico-legal problems. In this lecture, case studies will be presented that illustrate the nature of forensic anthropological casework where histological techniques have been helpful. 1:30-2:00 General Membership Meeting 2:00-2:30 Break 2:30-4:30 Forensic Case Studies in Histopathology Dr. James Terzian While many forensic cases are readily explained by crime scene, postmortem and toxicological analyses, there are times when microscopic tissue studies are used to determine the mechanism and /or manner of death. Based on one forensic pathologists experience, this lecture will give examples of the utility of histotechnology to forensic pathology. 5:00-7:00 Wine and Cheese Reception in the Exhibit Hall 7:00-11:00 Dinner and a Show, Enjoy the company of a few ?Folks? WORKSHOP SCHEDULE Saturday, May 7, 2003 8:00-10:00 Workshop A or B 8:00-11:30 Workshop C 10:00-10:30 Break 10:30-11:30 Workshop D, E 11:30-1:00 Awards Luncheon 1:00-4:30 Workshop G, H or I 2:30-3:00 Coffee Break Saturday May 7 2005 Morning Sessions: 8:00-10:00, 8:00-11:30 Select Workshop: A or B or C A. Artifacts Causes and Cures: Fixation, Processing, Sectioning: Peggy Wenk, (Limit 50, Basic, 2 contact hours) This seminar will present the cause of many different artifacts routinely observed in the histology laboratory. The solution to many of them will be demonstrated with the aid of photomicrographs. B. Microtomy: It?s About Technique: Mari Ann Maihoit (Limit 40, Basic, 2 contact hours) The purpose of this seminar is to bring about a better understanding of microtomy and the many factors that directly affect section quality. Instrument care as well as proper technique will be discussed so that techs can produce the best sections possible. Note: This workshop is from 8:00-11:30 C. Pathology for the Histotechnologist: Dr. Tom Haas (Limit 50, Advanced, 3 contact hours) This workshop will familiarize the uninitiated with terminology commonly used by surgical pathologist, and extend the application of these terms to the patterns commonly seen as histological features of tissues. Morning Sessions: 10:30-11:30 Select Workshop: D or E if taking either A or B D. Immunohistochemistry: The Good the Bad and The Tricky: Andrea Hooper (Limit 50, Intermediate, 1 contact hour) The use of ?same species? antibodies is becoming an increasing concern in both research and diagnostic immunopathology laboratories. This lecture will discuss some of the issues and solutions to working with same species antibodies in immunohistochemistry. E. Endless Possibilities for IHC using the Transfer Technique Kim Braczieswki / Melissa McCaffrey (Limit 50, Intermediate, 1 contact hour) Find out in this lecture how the ?Transfer Technique?, a simple and effective method, can be used to maximize limited tissue (including cytology specimens) for multiple applications Awards Luncheon 11:30-1:00 Enjoy a delicious lunch and celebrate the successes of your peers. The awards criteria were published in the ?On Stage? newsletter. We would like to recognize their contributions to the field of Histotechnology. Raffles held at the conclusion of the awards ceremony! Must be present to win. Afternoon Sessions: 1:00-4:30 Select One Workshop: G, H, or I G. Great Cases from Small Places: Dr. Tom Haas (Limit 50, Basic, 3 contact hours) This workshop will explore a number of different pathology cases form diagnoses through patient treatment to give a better understanding of the reasoning and rationale for the overall process. H. Affects of Fixation and Processing on IHC: Jerry Fredenburgh (Limit 50, Advanced, 3 contact hours) Fixation and epitope retrieval may be one of the most important variables in producing high quality Immunohistochemistry staining on paraffin or frozen sections. The effect of these factors will be discussed. I. Creating a Competency Assessment Program: Peggy Wenk Limit 50, Intermediate, 3 contact hours) Regulatory agencies require competency assessment of laboratory personnel but you have no idea where to begin!. This workshop will address writing detailed descriptions of tasks and behavior assessment, determining how competency needs to be addressed, setting-up evaluation systems and much, much more. Exhibitor Information Friday May 6, 2005: 12:00-5:00 Exhibitor Set-up From pruegg <@t> ihctech.net Thu Mar 31 15:38:34 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Tissues ffpe with long fixation time Message-ID: <200503312138.j2VLcVtr007852@chip.viawest.net> What extreme methods would you all recommend for getting at antigen sites on tissue that has been aldehyde fixed for nearly one month? I am working with pig heart samples, trying to do cd31, cd3, mac 387 all of which have been worked out wonderfully on my pig tonsil control tissue aldehyde fixed for a max of 48 hr. These are ffpe by the way. As mentioned the controls are staining beautifully but I get nothing on the samples of interest. Patsy From vazquezr <@t> ohsu.edu Thu Mar 31 15:44:13 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] National Histology Walkout Message-ID: Deborah, Did I miss something? Who said they were going to walk out? From what I have read they are going to boycott not renewing their ASCP stickers. Did I miss a message? Robyn >>> 03/31/05 12:56 PM >>> Wow, I must be living in a bubble in the Sacramento area. I personally don't feel victimized or taken advantage of in this profession. Is it just that I've been lucky for the past 11 years to experience respect as a histotech? I, for one, would NOT join a national walk-out for that reason. It's belief in patient care, the science and the art that I love so much about histology. I know doctors in this area respect their techs. Sincerely, Deborah King, HT(ASCP) Histology Supervisor Sacramento, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Mar 31 16:45:34 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] PTAH Mordant Message-ID: Please ignore "freat" and "do"........it has been a week! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Thu 3/31/2005 2:59 PM To: Joe Nocito; Histonet Cc: Subject: RE: [Histonet] PTAH Mordant We use the Zenker's that has zinc chloride instead of mercury. Works freat and do "de-zenkerization" required! We get ours from Newcomer Supply. Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, March 31, 2005 2:27 PM To: Histonet Subject: [Histonet] PTAH Mordant Hey y'all, since we no longer have Zenkers because of the mercury, what is being used as a mordant for the PTAH stain? My pathologists have been hounding me all week, but with my other net postings and replies, I just forgot. Thanks Histoland Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Mar 31 16:46:12 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Glass Coverslippers Message-ID: Definitely demo the Leica unit! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Grant, Debra Sent: Thu 3/31/2005 3:10 PM To: Histonet Cc: Subject: [Histonet] Glass Coverslippers Hi All, We would like to purchase a glass coverslipper very soon, and are interested in positive and negative feedback on all glass coverslippers. Thanks for your time in advance! P.S Is anyone demoing the Leica Coverslipper at the moment? Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org (913)926-4305 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Mar 31 16:46:56 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Glass Coverslippers Message-ID: We have the old Leica and like it very much. We are also about to demo the new unit. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Stacy McLaughlin Sent: Thu 3/31/2005 3:23 PM To: 'Grant, Debra'; Histonet Cc: Subject: RE: [Histonet] Glass Coverslippers Debby, We will be demoing the Leica Coverslipper next week. I'll be glad to let you know how it goes. Does anyone have any experience with the Sakura Glas coverslipper? We're trying that one in a few weeks. Thanks. Stacy McLaughlin -----Original Message----- From: Grant, Debra [mailto:DRG@Stowers-Institute.org] Sent: Thursday, March 31, 2005 3:11 PM To: Histonet Subject: [Histonet] Glass Coverslippers Hi All, We would like to purchase a glass coverslipper very soon, and are interested in positive and negative feedback on all glass coverslippers. Thanks for your time in advance! P.S Is anyone demoing the Leica Coverslipper at the moment? Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org (913)926-4305 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From M.Donovan <@t> alfred.org.au Thu Mar 31 17:02:18 2005 From: M.Donovan <@t> alfred.org.au (Donovan, Mark) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] CD2 Message-ID: <6B185007F2ABB44183969BCEECFCAED8379F54@alfmx03> Elizabeth, I have just been playing with a sample of CD2 antibody from Novocastra, Clone AB75, Cat NCL-CD2-271. Results have been very good on our routine FFPE tonsil controls using HIER Tris-EDTA pH 8 (Microwave Pressure Cooker) 3 min, 30 min primary incubation, Chemicon Select LSAB detection with Dako DAB+ chromogen. Beautiful membrane staining across a dilution range from 1 in 50 to 1 in 400. Staining was done using the Lab Vision Autostainer. An antibody worth looking at from what I have seen so far. Regards, Mark Donovan The Alfred Hospital Melbourne, Australia Original message Message: 10 Date: Thu, 31 Mar 2005 10:03:35 -0700 From: "Earle, Elizabeth" Subject: [Histonet] CD2 To: Message-ID: <4727B24D9A8575479222635B57E3B3DBCB1EB8@mail.vhswest.local> Content-Type: text/plain; charset="us-ascii" Does anyone know of a CD2 that works in FFPE tissue? Thanks E Earle THIS E-MAIL IS CONFIDENTIAL. If you have received this e-mail in error, please notify us by return e-mail and delete the document. If you are not the intended recipient you are hereby notified that any disclosure, copying, distribution or taking any action in reliance on the contents of this information is strictly prohibited and may be unlawful. Bayside Health is not liable for the proper and complete transmission of the information contained in this communication or for any delay in its receipt. From AnthonyH <@t> chw.edu.au Thu Mar 31 17:03:09 2005 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] PTAH Mordant Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3741269E207@simba.kids> Joe, We use the Cherukian's modification and it works well. Method as follows: Phosphotungstic Acid Haematoxylin Principle: Cherukian's modification employs an eosin solution that stains the erythrocytes red and differentiates them from the blue fibrin. Fixation: 10% buffered formalin. Microtomy: paraffin sections at 5um. Controls: Use brain sections and section of muscle. A good stain will demonstrate the dendrites as blue where as in a bad stain they appear light grey to salmon in colour. Nuclei, fibrin, platelets and muscle will be blue, red cells and collagen appear red. Muscle striations should be well defined. Reagents: 1. Eosin: - Warning: Flammable - see MSDS Eosin Y, water soluble (CI 145380) 0.5g Distilled Water 10ml 80% Ethanol 190ml Working: 10ml stock and Before use add 50ul glacial acetic acid. 2. 1% Periodic Acid 3. PTAH solution Haematoxylin (CI 75290) 0.5g Phosphotungstic Acid 10g Distilled water 500ml Dissolve solid ingredients in separate portions of the water. Use gentle heat for Haematoxylin. Combine solutions when cool. Add 0.088g potassium permanganate to ripen. The stain is ready to use. Procedure: 1. Dewax and hydrate sections to 80% alcohol. 2. Place slides in eosin for 30 seconds. 3. Wash slides in distilled water for a few seconds. 4. Place slides in 1% periodic acid for 20 minutes. 5. Wash slides in water for 3 minutes. 6. Place slides in PTAH for 30-90 minutes in 60oC oven. Check from 30 minutes on. 7. Dehydrate, clear and mount. Results: Dendrites, nuclei, fibrin, platelets and muscle - blue Red blood cells and collagen - red. Notes: Reference: 1. Cherukian, C.J., Histologic. 8(4); 105, (1977). 2. Luna, L., Histologic. 5(2); 66, (1975). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Joe Nocito [mailto:JNocito@Pathreflab.com] Sent: Friday, 1 April 2005 5:27 AM To: Histonet Subject: [Histonet] PTAH Mordant Hey y'all, since we no longer have Zenkers because of the mercury, what is being used as a mordant for the PTAH stain? My pathologists have been hounding me all week, but with my other net postings and replies, I just forgot. Thanks Histoland Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Thu Mar 31 17:43:57 2005 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:24:52 2005 Subject: FW: [Histonet] Orcein Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3741269E20A@simba.kids> -----Original Message----- From: wen eng [mailto:weneng2004@yahoo.com] Sent: Friday, 1 April 2005 9:31 AM To: Tony Henwood Subject: RE: [Histonet] Orcein Thank you so much! I really appreciate your big help! Wen Tony Henwood wrote: Wen, I have attached an article from Biotechnic & Histochemistry 2003, 78(6): 303-308. "Current applications of orcein in histochemistry. A brief review with some new observations concerning influence of dye batch variation and aging of dye solutions on staining" That might be of use Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: wen eng [mailto:weneng2004@yahoo.com] Sent: Wednesday, 30 March 2005 7:50 AM To: histonet Subject: [Histonet] Orcein Hi, I was asked to do Orcein stain for elastic fibres. I couldn't find method. Could anybody tell me where I ! can find it or send me a copy? I really apperciate any input. Wen --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _____ Do you Yahoo!? Yahoo! Mail - Easier than ever with enhanced search. Learn more. From c.gorrie <@t> unsw.edu.au Thu Mar 31 18:08:02 2005 From: c.gorrie <@t> unsw.edu.au (Cath Gorrie) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] 2) Immunofluorescence and background In-Reply-To: <8362520.1112176318135.JavaMail.jerry.lyons@ucd.ie> Message-ID: I have a different but similar problem with Immunofluorescence and background. I'm cutting rat spinal cord sections that have been perfused and post fixed in 4% paraformaldehyde, cryoprotected and cut as frozen sections. The autofluorescence in the tissue is so high I am unable to clearly see fluorescent labelled cells. Is this a problem of the fixation in 4% paraformaldehyde? or a property of spinal cord? or is there something obvious I should or shouldn't be doing? Regards, cath ---------------------------------------------------------------------------- Catherine Gorrie School of Medical Sciences University of New South Wales Sydney, NSW ph: 02 9385 2462 fax: 02 9385 8016 e-mail: c.gorrie@unsw.edu.au ---------------------------------------------------------------------------- From lpwenk <@t> sbcglobal.net Thu Mar 31 18:26:45 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] PTAH Mordant References: Message-ID: <006b01c53651$7d68d3c0$50d7d445@domainnotset.invalid> We use Bouin fixative - 60 degree oven for 1 hour (after deparaffinizing slides and running them down to water). (Can skip the Lugolizing steps) Then wash in running water until yellow disappears from the tissue (1-10 minutes, depending on type of tissue. The more striated muscle there is, the longer it takes.) Then stain as usual in PTAH. We get better, more consistent staining with this method. One of my fellow students found it when we were in training. We had to do a PTAH for our practical. Worked great. I believe it was from the Ann Preece book. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Joe Nocito" To: "Histonet" Sent: Thursday, March 31, 2005 2:27 PM Subject: [Histonet] PTAH Mordant > Hey y'all, > since we no longer have Zenkers because of the mercury, what is being used > as a mordant for the PTAH stain? My pathologists have been hounding me all > week, but with my other net postings and replies, I just forgot. Thanks > Histoland > > > Joe Nocito, BS, HT(ASCP) QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katri <@t> cogeco.ca Thu Mar 31 19:25:51 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] email problem Message-ID: <000301c53659$be592670$6a9a9618@Katri> I'm having problems receiving majority of my emails, particularly from histonet server. Could you send me an email to see if it comes through, please... Katri From lpwenk <@t> sbcglobal.net Thu Mar 31 19:30:39 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] ASCP BOR - answers to questions Message-ID: <00b201c5365a$6a3d83a0$50d7d445@domainnotset.invalid> Going to try to answer several questions in this one email. If not interested in ASCP BOR or in some answers - please push the delete button now. 1. WHO TO TALK TO ABOUT COMPLAINTS ABOUT ASCP BOARD OF REGISTRY (BOR) The NSH appointed representative to ASCP BOR is Marilyn Gamble. There is a link to her email at the NSH web site http://www.nsh.org - click on Who We Are - click on Appointments - click on Marilyn's email link under ASCP BOR The list of histotechs and pathologists on the ASCP BOR Histotechnology exam committee can be found on the ASCP BOR webpage. There are no email links to their names. http://www.ascp.org/bor/about - click on Histotechnology Examination Committee Also, you can contact ASCP BOR directly via phone or email. You could ask them a question, or ask for the phone number on the Histotechnology exam committee. The list of staff people working at the BOR and their roles can be found on: http://www.ascp.org/bor/about/ - at the bottom of the page, click on Contact an ASCP Board of Registry staff member. 2. WHY THE INCREASE IN THE COST OF GRADING HT/HTL PRACTICAL EXAMS? Well, the ASCP BOR staff member was at NSH S/C in Toronto, and talked about it at the booth, and at several committee meetings. I also called ASCP BOR in the fall for additional information, which they gave out. Nothing hidden or secret about it. Ask, and they will tell. The HT and HTL exams are the most expensive technician/technologist exams for ASCP BOR, because HT/HTL/pathologists graders have to be brought in from all other the USA, two weekends a year, to grade all the sets. The fee paid by the HT/HTL candidates did not cover the cost of the ASCP grading the written (computer) and the practical HT/HTL exams. ASCP BOR had made several changes recently, to keep the cost down. Instead of submitting 15 slides, BOR statistically found that 9 slides were sufficient to achieve the same pass/fail rate as 15 slides. (In other words, if someone does a good job on cutting and staining 9, they would have done the same good job on 15. And the same with someone doing a not so good/bad job.) By reducing the number of slides, they reduced the number of people needed to grade the slides, thereby reducing costs. A new change is that the candidate must pass the written/computer portion first, before they can submit their practical. This will reduce the number of practicals being sent in. This again will reduce the number of graders needed, which will keep costs down. However, unfortunately, these changes could not keep the costs down enough, and now ASCP BOR is asking the candidates to help to cover SOME of the costs of grading the practical exams, by paying an additional $75. Notice the word SOME. This additional fee still does not cover all the costs. 3. WHAT DO I, AS A HISTOTECH, GET BY BEING AN ASCP MEMBER? Maybe not as much as being an NSH member, but I still think I get some things. - Tech Samples (for continuing education) - ASCP Teleconferences (at least 3 each quarter are histology. Others on management are also helpful) - "Laboratory Medicine" (which might not have as much histology articles as I might like, but it gives me a better understanding of how all the labs fit in together. Helpful in management issues.) - Representatives from ASCP in Washington DC, working on: laws for payment and reimbursement which effect my pay; getting grants to start new lab schools; etc. - Books like the Frieda Carson textbook, HT/HTL exam study books, Jules Elias book on IHC - Annual wage and salary survey And all of these things cost money to set up and run. Money from ASCP members. All of the above information I obtained by A) calling ASCP BOR and asking them questions I have, B) attending committee meetings at NSH where the ASCP BOR staff are there to answer questions, and C) talking with the ASCP BOR staff that are at the ASCP BOR booth in the exhibit hall that the NSH S/C, D) reading the ASCP BOR web page, "Laboratory Medicine", and anything else I can get. It's not a secret. And calling does help. Sometimes it takes a while, but things do change. I've called the NSH representatives to ASCP BOR and the chair of the ASCP BOR Histotechnology exam committee in the past with concerns, ideas, suggestions, questions. I've gotten answers, and often they have incorporated my suggestions into changing things on the exams (different tissues, stains, checking on a question when my students swear there wasn't a right answer (typo, it turned out, which the BOR QC had already caught so the students' scores were not effected, etc.)). I've raised questions about the wage and salary survey, and that was modified. I've offered suggestions about their flyers about histotechs, and these were changed in the next revision (about 4 years later - slowly, remember?). No, they didn't change these things because it was ME who called. They changed because I offered a good suggestion. There are also things they did not change. So it goes. No, it isn't perfect. But from where I stand, I think they need more constructive input and more people being involved. That's us, people. We care about our field, otherwise we wouldn't still be in the field and on Histonet. So let's be constructive and offer concrete suggestions and ideas for improvement to our representatives - email, call, and attend the NSH Education Committee or Instructors in Histotechnology meeting in Fort Lauderdale. It takes more than 6 people sitting in on a committee, to make changes. It's sad when the meetings are open to everyone of the 1200 people in attendance, and only 6 people show up. (That is my personal opinion, not something gleaned from a book or webpage.) Off my soap box for a while. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From RCHIOVETTI <@t> aol.com Thu Mar 31 20:53:57 2005 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] 2) Immunofluorescence and background Message-ID: <12b.5a05ef28.2f7e11c5@aol.com> In a message dated 3/31/2005 5:11:55 PM US Mountain Standard Time, c.gorrie@unsw.edu.au writes: > have a different but similar problem with Immunofluorescence and > background. I'm cutting rat spinal cord sections that have been perfused and > post fixed in 4% paraformaldehyde, cryoprotected and cut as frozen sections. > > Hi Cath, Yes, it's almost certainly autofluorescence due to the aldehyde fixation...perhaps you can cut down on the fluorescence by shortening the fixation time, or you can try using sodium borohydride treatment on the sections before beginning the staining protocol, or perhaps quenching the reactive groups with amines (like lysine.) The subject has been discussed a few times on Histonet. You can search the archives by going to: Use the search terms "autofluorescence paraformaldehyde" (w/o quote marks) to see what's been said about the problem. Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Independent Consultant for The Science, Technology and Industrial Sectors 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Member, Arizona Small Business Association - ASBA From zumbor <@t> email.cs.nsw.gov.au Thu Mar 31 21:11:41 2005 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] PTAH MORDANT Message-ID: <002e01c53668$8697a130$1e7b4c98@dofm.cs.nsw.gov.au> We use bouins for 1 hour at 60 degrees as mordant for PTAH.Rosalba Zumbo Laboratory Manager Histology Dept. Department of Forensic Medicine 42-50 Parramatta rd Glebe, 2037 Australia Ph: 61 2 85847842 Fax: 61 2 95664573 email: zumbor@email.cs.nsw.gov.au "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From marktarango <@t> earthlink.net Wed Mar 23 15:53:33 2005 From: marktarango <@t> earthlink.net (Mark Tarango) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: Histonet Digest, Vol 17, Issue 1 References: <200504010820.1dhoSHW33NZFpN0@mx-a065b14.pas.sa.earthlink.net> Message-ID: <001a01c52ff2$c2b01d40$29d1df42@TARANGO> I want to know why the ASCP is refusing to tell me what my certification number is. I pass their ridiculous test 1st try. I don't pay their dues every year because it's a ripoff. While I'm here in Alsaka, I need to know my number. I have it written down back home, but I'm not there now. They'll only tell me my number if i pay them their yearly fees. I passed the exam, so why are they bullying me here? Maybe if they worked a little harder on our wages or getting us some respect in the lab, I could afford it or wouldn't mind paying it. Mark Tarango > Message: 9 > Date: Thu, 31 Mar 2005 20:30:39 -0500 > From: > Subject: [Histonet] ASCP BOR - answers to questions > To: "Histonet" > Message-ID: <00b201c5365a$6a3d83a0$50d7d445@domainnotset.invalid> > Content-Type: text/plain; charset="iso-8859-1" > > Going to try to answer several questions in this one email. If not > interested in ASCP BOR or in some answers - please push the delete button > now. > > 1. WHO TO TALK TO ABOUT COMPLAINTS ABOUT ASCP BOARD OF REGISTRY (BOR) > > The NSH appointed representative to ASCP BOR is Marilyn Gamble. There is a > link to her email at the NSH web site > > http://www.nsh.org > - click on Who We Are > - click on Appointments > - click on Marilyn's email link under ASCP BOR > > The list of histotechs and pathologists on the ASCP BOR Histotechnology > exam > committee can be found on the ASCP BOR webpage. There are no email links > to > their names. > > http://www.ascp.org/bor/about > - click on Histotechnology Examination Committee > > Also, you can contact ASCP BOR directly via phone or email. You could ask > them a question, or ask for the phone number on the Histotechnology exam > committee. The list of staff people working at the BOR and their roles > can > be found on: > > http://www.ascp.org/bor/about/ > - at the bottom of the page, click on Contact an ASCP Board of Registry > staff member. > > > 2. WHY THE INCREASE IN THE COST OF GRADING HT/HTL PRACTICAL EXAMS? > > Well, the ASCP BOR staff member was at NSH S/C in Toronto, and talked > about > it at the booth, and at several committee meetings. I also called ASCP BOR > in the fall for additional information, which they gave out. Nothing > hidden > or secret about it. Ask, and they will tell. > > The HT and HTL exams are the most expensive technician/technologist exams > for ASCP BOR, because HT/HTL/pathologists graders have to be brought in > from > all other the USA, two weekends a year, to grade all the sets. > > The fee paid by the HT/HTL candidates did not cover the cost of the ASCP > grading the written (computer) and the practical HT/HTL exams. > > ASCP BOR had made several changes recently, to keep the cost down. > > Instead of submitting 15 slides, BOR statistically found that 9 slides > were > sufficient to achieve the same pass/fail rate as 15 slides. (In other > words, > if someone does a good job on cutting and staining 9, they would have done > the same good job on 15. And the same with someone doing a not so good/bad > job.) By reducing the number of slides, they reduced the number of people > needed to grade the slides, thereby reducing costs. > > A new change is that the candidate must pass the written/computer portion > first, before they can submit their practical. This will reduce the number > of practicals being sent in. This again will reduce the number of graders > needed, which will keep costs down. > > However, unfortunately, these changes could not keep the costs down > enough, > and now ASCP BOR is asking the candidates to help to cover SOME of the > costs > of grading the practical exams, by paying an additional $75. Notice the > word > SOME. This additional fee still does not cover all the costs. > > > 3. WHAT DO I, AS A HISTOTECH, GET BY BEING AN ASCP MEMBER? > > Maybe not as much as being an NSH member, but I still think I get some > things. > - Tech Samples (for continuing education) > - ASCP Teleconferences (at least 3 each quarter are histology. Others on > management are also helpful) > - "Laboratory Medicine" (which might not have as much histology articles > as > I might like, but it gives me a better understanding of how all the labs > fit > in together. Helpful in management issues.) > - Representatives from ASCP in Washington DC, working on: laws for payment > and reimbursement which effect my pay; getting grants to start new lab > schools; etc. > - Books like the Frieda Carson textbook, HT/HTL exam study books, Jules > Elias book on IHC > - Annual wage and salary survey > > And all of these things cost money to set up and run. Money from ASCP > members. > > All of the above information I obtained by A) calling ASCP BOR and asking > them questions I have, B) attending committee meetings at NSH where the > ASCP > BOR staff are there to answer questions, and C) talking with the ASCP BOR > staff that are at the ASCP BOR booth in the exhibit hall that the NSH S/C, > D) reading the ASCP BOR web page, "Laboratory Medicine", and anything else > I > can get. It's not a secret. > > And calling does help. Sometimes it takes a while, but things do change. > I've called the NSH representatives to ASCP BOR and the chair of the ASCP > BOR Histotechnology exam committee in the past with concerns, ideas, > suggestions, questions. I've gotten answers, and often they have > incorporated my suggestions into changing things on the exams (different > tissues, stains, checking on a question when my students swear there > wasn't > a right answer (typo, it turned out, which the BOR QC had already caught > so > the students' scores were not effected, etc.)). I've raised questions > about > the wage and salary survey, and that was modified. I've offered > suggestions > about their flyers about histotechs, and these were changed in the next > revision (about 4 years later - slowly, remember?). No, they didn't change > these things because it was ME who called. They changed because I offered > a > good suggestion. There are also things they did not change. So it goes. > > No, it isn't perfect. But from where I stand, I think they need more > constructive input and more people being involved. That's us, people. We > care about our field, otherwise we wouldn't still be in the field and on > Histonet. So let's be constructive and offer concrete suggestions and > ideas > for improvement to our representatives - email, call, and attend the NSH > Education Committee or Instructors in Histotechnology meeting in Fort > Lauderdale. It takes more than 6 people sitting in on a committee, to > make > changes. It's sad when the meetings are open to everyone of the 1200 > people > in attendance, and only 6 people show up. (That is my personal opinion, > not > something gleaned from a book or webpage.) > > Off my soap box for a while. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital From marktarango <@t> earthlink.net Wed Mar 23 15:56:44 2005 From: marktarango <@t> earthlink.net (Mark Tarango) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Paying the high ASCP fees each year References: <200504020854.1dhLSO6fn3NZFpr0@mx-a065a01.pas.sa.earthlink.net> Message-ID: <000c01c52ff3$3483abd0$29d1df42@TARANGO> Why pay ASCP to bully me around? I pay NSH, and CSH(California) every year because its worth it, and they don't try and force you into it. I like reading the JOH, that alone makes the NSH dues worthwhile. ASCP, I won't pay. They're not even friendly on the phone. Why can't the NSH just start certifying people? Mark Tarango >Message: 23 >Date: Sat, 2 Apr 2005 09:52:14 -0700 >From: "Patsy Ruegg" >Subject: [Histonet] participation >To: "'Mark Tarango'" , > >Message-ID: <200504021652.j32GqscS088263@pro12.abac.com> >Content-Type: text/plain; charset="us-ascii". >I must chime in here a bit. Those who do not participate in the their >professional organizations (ASCP, NSH, state societies, etc.) are missing >out on resources that they of course don't know anything about because they >are not engaged. Even when you do pay ASCP dues each year the benefits are >not always transparent. ASCP is the Certifying agency for our profession, >without them none of us would have documentation that we are >"professionals". I know that there are plenty of people out there who may >have been working in the field for a long time and can perform the required >tasks, but the fact that they have not gotten certified says something >about >them that frankly would not impress me as a perspective employer. With a >few exceptions (ie Jackie O, who is certified I know) I would be willing to >bet that these non-certified, non-engaged people do not read the Journals >or >attend classes in their field, which again would not impress me as their >employer. >It is our job to educate the administrators. They often have no idea that >non-certified people are working for them or even that certification is >available in some cases. NSH has been working for many years to try and >get >CAP to require certified Histotech's be working in the histo lab, we even >asked that they at least require supervisors be certified. CAP still has >no >such requirements. It can be a long tuff battle, but we are making >progress. ASCP, NSH, CAP and State Histology Societies, to mention a few, >are our professional bargaining agencies. Through them we will make the >changes in the field we want to see. Without these agencies we are just >individuals whining about issues we are doing nothing to affect. >Best regards, >Patsy Ruegg