[Histonet] help for brazilian student

[Flavia Danielski]

Hello...
I would be really glad if someone could help me with
XGal staining for tibia... the protocols that I have
work well for soft tissues, but not for bone. How
should I process the sample? I need to obtain sections
of the bone ...  should I do it before XGal staining
or after??? If after, how should I process the slices
to reveal beta gal? Does the parafine interfire with
the process?
Thank you very much and greetings from Brazil
sorry for my bad english
Flavia

Flávia Helena da Silva
Lab. de Imunogenética, Depto. de Genética, UFRGS 
Av. Bento Gonçalves, 9500 prédio 43323/lab 212C
Bairro Agronomia.
POA-RS-BRAZIL
phone: 0055-(51)33166737


	
	
		
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