[Histonet] OCT

[Lewis, Sarah]

Hello! 

I use 7% gum Tragacanth that I make up myself using 100ml of distilled water, 7gms of gum Tragacanth, and 5 grains of Thymol. Dissolve by manually stirring at rm temp.(may take several hours) Store half refrigerated(4c) and half frozen  
 (-20c). I work with only muscle and nerve biopsy tissue but this works great for me. I am sure this is a bit "old school", but I have never had any trouble with cracking or stability of the tissue. It also seems to be great for long term storage in -80 freezer. Good luck!!
 

Sarah E. Lewis 
Histotechnician 
Childrens Research 
Center for Gene Therapy 
700 Childrens Dr Rm WA3112 
Columbus OH 43205 
(614)-722-2204 
LewisS <@t> ccri.net 

 

-----Original Message----- 
From: ssq5977 <@t> yahoo.com.cn [mailto:ssq5977 <@t> yahoo.com.cn] 
Sent: Thursday, June 16, 2005 9:30 PM 
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Is there anything better than OCT? 



Hi all: 

We are currently using OCT to embed renal biopsy tissue in cryostat mold. First of all ,we put a drop of OCT on the mold ,then we put the tissue on the top of the OCT and dip the mold into liquid nitrogen for 10 seconds. then the tissue is sent to cryosection. Does anyone know if there is anything better than OCT to embed the tissue? 

Thanks! 

Shuqiong Shen(ssq5977 <@t> yahoo.com.cn) 
Research Institute of Nephrology, Jinling Hospital 
305 East Zhongshan Road, Nanjing 
Jiangsu Province,P.R of China 

Zip code: 210002 
Tel: +86 25 85912177 




Sarah E. Lewis 
Histotechnician 
Childrens Research 
Center for Gene Therapy 
700 Childrens Dr Rm WA3112 
Columbus OH 43205 
(614)-722-2204 
LewisS <@t> ccri.net 

 

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