[Histonet] RE: Blocking serum

[C.M. van der Loos]

   Dear Baowei Peng,

   Since  you  have  a  goat  anti-rabbit  IgG as second step reagent you
   should  use  normal  goat  serum  for pre-blocking step. Otherwise use
   Protein Block, serum-free (DakoCyto X0909).

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389

   Date: Wed, 29 Jun 2005 08:35:04 +0800 (BEIST)
   From: Baowei Peng <pengbw <@t> sjtu.edu.cn>
   Subject: [Histonet] Blocking serum
   To: histonet <@t> lists.utsouthwestern.edu
   Message-ID: <20050629003504.7EDCD11104FD <@t> sjtu.edu.cn>
   Content-Type: text/plain; charset="gb2312"
   Hello, all
   I\'m doing IHC on mouse frozen sections. The first antibody in my hand
   is  Rabbit polyclonal antibody,the second antibody is Goat anti Rabbit
   IgG(H+L).  I\'m wondering what kind of serum I should use to block non
   specific staining.
   Thanks in advance!
   Baowei Peng
   1954 Huashan Road
   Pharmacy School
   Shanghai Jiaotong University
   Shanghai, 200030
   China
   Ph:86-21-62932108
   E-mail:pengbw <@t> sjtu.edu.cn