[Histonet] Problem with CC1 on Benchmark

[White, Lori]

Bill,
We have also been experiencing non-specific staining and do not use CC1
but retrieve offline.  The problem appears to be with specific lot # of
detection kits.  Ventana is aware of this problem and should be working
with you to resolve it.
Good luck!
 
Lori 
 
Message: 5
Date: Tue, 28 Jun 2005 11:16:52 +1000
From: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>
Subject: [Histonet] Problem with CC1 on Benchmark.
To: "histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c57b7f$11f8aca0$0ecd080a <@t> wsahs.nsw.gov.au>
Content-Type: text/plain;     charset="iso-8859-1"
 
 
Dear All,
 
Particularly those using Ventana IHC Stainers.
 
We have a Ventana Benchmark and recently (from the middle of May) we
have a problem with antibodies which have had a pre-treatment in CC1.
 
1     After CC1, whether mild or standard we are getting non-specific
biotin
type staining.  However, when you look at the slides they  look specific
in their staining although for the wrong antibody.  The negative
controls are displaying this phenomenon as well.
 
2.    The staining can be blocked with the Avidin-Biotin block, but this
does
not always remove the staining completely.
 
We are using Batch #'s 48091 & 48127 of CC1.
 
Any advice would be greatly appreciated.
 
Thanks
Jane Milliken,
IHC Benchmark Driver
 
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
 
 
 
Lori White, B.Sc., MLT
Charge Technologist, Histopathology
Lakeridge Health Corporation
Tel   905 576-8711 ext. 4358
Fax  905 905 721-4757