[Histonet] OIL RED O - Mounting Media

[mpaula]

I mounted my fat slide with a mounting medium specific for Oil Red O

The Protocol is:

Apathy's Mouting Media

50 g sucrose PA
50 g Acacia (gum arabic) PA
150 ml Distilled Water
0.1 g Thymol
Mix Acacia and distilled water in a Becker. Place the Becker in a pan of
boiling water until the Acacia is dissolved. Than mix sucrose until dissolved and
put in a Thymol. Refrigerate to remove air bubbles.

Carefful with bubbles.

Thanks

MariaPaula Montiani Scandar
Department of Pathology
Labory of Neuropathology
School of Medicine of Ribeirão Preto
University of São Paulo
Brazil



----- Original Message -----
From: "Anita Jennings" <ajennings <@t> unmc.edu>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 22, 2005 10:42 AM
Subject: [Histonet] Oil Red O mountant


>
>
>
>
> Andi
> I had the same problem. initially with my fat stain.....Poly Scientific
kit
> K043 contains the glycerine jelly mounting medium specific for Oil Red
> coverslipping, havent had a problem since I started ordering this kit
> 800-645-5825
>
>
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