[Histonet] Re: Histonet Digest, Vol 19, Issue 37

[Lightbown, Angela]

On Tuesday, June 28, 2005, at 08:21 AM,  
histonet-request <@t> lists.utsouthwestern.edu wrote:

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> Today's Topics:
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>    1. S100 (Laura Fidgen)
>    2. PAR staining - Japanese source? (JonesLy <@t> mir.wustl.edu)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 24 Jun 2005 13:50:18 -0400
> From: Laura Fidgen <lfidgen <@t> vt.edu>
> Subject: [Histonet] S100
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <6.0.0.22.0.20050624134204.0271bec0 <@t> pop.vt.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> We are a Veterinary Hospital evaluating a Ventana Benchmark LT.  I am
> having a lot of problems staining for S100.  It is very dark with
> background.  The people at Ventana think the secondary antibody is
> cross-reacting and suggest using a different secondary (our secondary
> currently is goat/rabbit).  Can anyone in veterinary medicine help me  
> out
> with this?  So far my experience with this machine has not been
> favorable.  Any advice would be appreciated.
>
> Laura L. Fidgen, MScF, BSc, HT(ASCP)
> Laboratory and Research Practioner
> VMRCVM, Histopatholgy
> Blacksburg, VA 24060-0443
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 24 Jun 2005 13:13:12 -0500
> From: JonesLy <@t> mir.wustl.edu
> Subject: [Histonet] PAR staining - Japanese source?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<OF758EED14.9D5887D5-ON8625702A.0061CC82- 
> 8625702A.00641606 <@t> msnotes.wustl.edu>
> 	
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>
>
> Hello -
> The person who started this project is currently in China, but my PI  
> asked
> if I could query the list anyway - apologies if my question is vague.   
> I
> collect the tissues we stain, but have little involvement in their
> subsequent staining and am NOT a histologist.
>
> We need to stain formalin-fixed parafin embedded tissue (from
> streptozotocin-treated rats) for PAR, Poly-ADP-ribose - not PARP,
> poly-ADP-ribose polymerase.  When we started this project, we had an
> antibody that gave beautiful results; unfortunately subsequent lot  
> numbers
> show poor staining of our control block.  (The vendor has acknowleded  
> that
> the problem is on their end, but that doesn't help our research.)   
> While at
> a meeting in DC, the PI spoke with someone who was also working with  
> PAR
> and has excellent results using a stain (monoclonal antibody?) from a
> vendor in Japan.  It is apparently expensive, but gives very  
> reproducable
> results.  The person working with this stain was supposed to e-mail  
> our PI
> with the details, but he hasn't heard from her.
>
> Even if no-one has worked with the stain, a list of Japanese suppliers  
> of
> antibodies would help.  (Again, we need to stain for PAR, not PARP.)
> Thanks in advance,
> Lynne Jones
>
> Senior Research Technician
> Dept. of Radiological Sciences
> Washington University Medical School
> St. Louis, MO
>
> ------------------------------
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> End of Histonet Digest, Vol 19, Issue 37
> ****************************************
>
>
Angela Lightbown
Clinical Applications/IHC
Cell Signaling Technology
166B Cummings Center
Beverly, MA 01915

phone: (978) 867-2440
email:  alightbown <@t> cellsignal.com