[Histonet] Re: S100 Staining

[Brian Chelack]

Hello Laura;

Regarding the Ventana secondaries in veterinary species, If
I assume that you are trying this staining on canine
tissues, our experience has been similar. The Ventana
secondaries do have a tendancy to crossreact with tissue
components (immunoglobulin)from some small animal species.
It does limit the usefulness of this platform for small
animal tumour markers, you should see how much fun it is for
B-cell markers...

regards,

Brian Chelack
Prairie Diagnostic Services


> Date: Fri, 24 Jun 2005 13:50:18 -0400
> From: Laura Fidgen <lfidgen <@t> vt.edu>
> Subject: [Histonet] S100
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <6.0.0.22.0.20050624134204.0271bec0 <@t> pop.vt.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
> 
> We are a Veterinary Hospital evaluating a Ventana Benchmark LT.  I am
> having a lot of problems staining for S100.  It is very dark with
> background.  The people at Ventana think the secondary antibody is
> cross-reacting and suggest using a different secondary (our secondary
> currently is goat/rabbit).  Can anyone in veterinary medicine help me out
> with this?  So far my experience with this machine has not been
> favorable.  Any advice would be appreciated.
> 
> Laura L. Fidgen, MScF, BSc, HT(ASCP)
> Laboratory and Research Practioner
> VMRCVM, Histopatholgy
> Blacksburg, VA 24060-0443
> 
***