[Histonet] S100

[Laura Fidgen]

We are a Veterinary Hospital evaluating a Ventana Benchmark LT.  I am 
having a lot of problems staining for S100.  It is very dark with 
background.  The people at Ventana think the secondary antibody is 
cross-reacting and suggest using a different secondary (our secondary 
currently is goat/rabbit).  Can anyone in veterinary medicine help me out 
with this?  So far my experience with this machine has not been 
favorable.  Any advice would be appreciated.

Laura L. Fidgen, MScF, BSc, HT(ASCP)
Laboratory and Research Practioner
VMRCVM, Histopatholgy
Blacksburg, VA 24060-0443