[Histonet] CDX2

[Riesen, Rebecca]

	We have had no trouble at all with this antibody from BioGenex.  I use Benchmark XT.  We do Mild pretreatment with CC1 for 30 minutes and incubate the primary at 42 degrees for 16 min, with a standard detection kit.  Good Luck!
	
	Message: 12
	Date:	Wed, 22 Jun 2005 13:57:21 -0500
	From:	"Bauer, Karen" <Bauer.Karen <@t> mayo.edu>
	Subject:	[Histonet] CDX2
	To:	<histonet <@t> lists.utsouthwestern.edu>
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		<FAEEA161EC98C6439D4892E161BCE18AD320EF <@t> LMMAILVS1.ad.lmmhs.org>
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	Does anyone in histoland have a working protocol for CDX2?  I have a
	pre-dilute from BioGenex that I'm trying to use on our BenchMark, but
	nothing seems to work.  I've called Ventana and BioGenex and have tried
	their suggestions, but with no luck.  Anybody willing to share their
	protocol?  I'd love to give it a try.

	Thanks much!!
	Karen Bauer HT(ASCP)
	Histology Supervisor
	Luther Hospital 
	Eau Claire, WI
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