[Histonet] RE: Histonet Digest, Vol 19, Issue 30

[Riesen, Rebecca]

	We use eosin in the processor and find that if it is in anything earlier than the second absolute it washes out too much.  So, I would guess that eosin prior to putting the tissues on the processor wouldn't work.  There are many other dyes that are made for the purpose you are proposing.  We use Bradley products, but there are others out there.  
	Message: 9
	Date:	Mon, 20 Jun 2005 08:47:44 -0700 (PDT)
	From:	Steven Coakley <sjchtascp <@t> yahoo.com>
	Subject:	[Histonet] tissue dye
	To:	histonet <@t> lists.utsouthwestern.edu
		Message-ID: <20050620154744.73767.qmail <@t> web90202.mail.scd.yahoo.com>
		Content-Type: text/plain; charset=iso-8859-1

					Good morning all,
	I have heard of some techs using eosin in an alcohol of the tissue processor to dye tissues.
	I'd like to avoid this, but would like to dye specific tissues for easier orientation.  Would a dilute eosin work if used prior to putting the tissues on the processor?  If so, aqueous or alcoholic?  What percentage?
	Steve
			
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