[Histonet] Frozen sections for IF

[Catherine Stanecki]

Hi Histonetters,

  So, I'm pretty new to histology and I have several questions about frozen
sections.  I would really appreciate your help!  First of all, how long are
frozen tissues good for after freezing in tissue tek OTC?  Is it better to
store at -20 C or -80 C?  Also, how long are sections on slides good for
before you have stained and how should you store them (i.e. in a slide box
in the freezer)?  How far in advance can you cut your sections before
staining them?

  Thanks so much for you help!

Cathy