[Histonet] Brain fixation
mary johnson
beingmary53 <@t> sbcglobal.net
Sun Jun 19 12:47:20 CDT 2005
Hi. Dorothy we fix our brain section in 20% formalin, and if your section is
thin enough in two to three days they are ready for prosses oh yes the 20%
for formalin is what we make up.
Good luck
mary Johnson HT ascp
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, June 16, 2005 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 19, Issue 23
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Today's Topics:
1. Brain fixative (Webb, Dorothy L)
2. thanks (Kaye Ryan)
3. RE: Brain fixative[Scanned] (Rogerson Kemlo (ELHT) Pathology)
4. Processing of research/mouse tissues (was delayed processing)
(Johnson, Teri)
5. Re: Cryostat sections with small bubbles (Kathleen Spencer)
6. Negatives for immunos using polyclonal antibody
(andrew.macduff <@t> ed.ac.uk)
7. RE: Schiff's reagent in microwave? (Bonner, Janet)
8. Microwave use in Pharmaceutical/CRO histo labs
(Stephen.Eyres <@t> sanofi-aventis.com)
9. Required CEU's (Dianne Holmes)
10. Muscle Referrals @ Baylor - Houston (Nita Searcy)
11. One of our own in need ! Please Read (Vinnie Della Speranza)
12. RE: Schiff's reagent in microwave? (GUTIERREZ, JUAN)
13. RE: Brain fixative (GUTIERREZ, JUAN)
14. RE: BCIP/NBT ready-to-use (Edmondson David (RBV) NHS Christie Tr)
15. RE: Schiff's reagent in microwave? (mucram11 <@t> comcast.net)
----------------------------------------------------------------------
Message: 1
Date: Thu, 16 Jun 2005 09:52:51 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Brain fixative
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<0E394B648E5284478A6CCB78E5AFDA2718C368 <@t> hpes1.HealthPartners.int>
Content-Type: text/plain; charset="US-ASCII"
Does anyone fix their brain tissue from autopsies in anything other than
10% buffered formalin? The PA I am working with seems to feel they used
a 4% solution of lightly buffered formalin from Mallincroft. Can anyone
help me with this, as I am only used to using 10% formalin, as we use
for all of our routine histology fixation. Any ideas would be greatly
appreciated and thanks ahead of time to all of my fellow
histonetters..what a great group you are!!
+______________________________________+
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Message: 2
Date: Thu, 16 Jun 2005 10:58:17 -0400
From: "Kaye Ryan" <ryakay <@t> shands.ufl.edu>
Subject: [Histonet] thanks
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s2b15b60.012 <@t> GW-FS1.SHANDS.UFL.EDU>
Content-Type: text/plain; charset=US-ASCII
Thanks to everyone who responded to my inquiry concerning decal
solutions for bone marrows.
Kaye
Kaye Ryan
Histology Manager/Educational Coordinator
Rocky Point Laboratories
265-0111, 72093
------------------------------
Message: 3
Date: Thu, 16 Jun 2005 16:00:08 +0100
From: "Rogerson Kemlo (ELHT) Pathology" <Kemlo.Rogerson <@t> elht.nhs.uk>
Subject: RE: [Histonet] Brain fixative[Scanned]
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<53A22BBB01D6184EBF7A4DC18A021E9E5AD2D1 <@t> elht-exch1.xelht.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
Aren't they the same? We've had this debate; 4% formaldehyde is 10%
formalin. 'Lightly buffered' is that like lightly salted?
-----Original Message-----
From: Webb, Dorothy L [mailto:Dorothy.L.Webb <@t> HealthPartners.Com]
Sent: 16 June 2005 15:53
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Brain fixative[Scanned]
Does anyone fix their brain tissue from autopsies in anything other than
10% buffered formalin? The PA I am working with seems to feel they used
a 4% solution of lightly buffered formalin from Mallincroft. Can anyone
help me with this, as I am only used to using 10% formalin, as we use
for all of our routine histology fixation. Any ideas would be greatly
appreciated and thanks ahead of time to all of my fellow
histonetters..what a great group you are!!
+______________________________________+
________________________________________
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is strictly prohibited.
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------------------------------
Message: 4
Date: Thu, 16 Jun 2005 10:00:51 -0500
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Processing of research/mouse tissues (was delayed
processing)
To: <sjchtascp <@t> yahoo.com>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1118941200.20804.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="US-ASCII"
Steve, welcome to research! It is a different world here. My advice
would be to buy a hydrometer, and test your alcohols. When your last
100% starts to show some water, rotate it up to the position just past
the 95% alcohol and make the later changes fresh. Also invest in the
animal processing manual from the NSH. You'll find some very helpful
schedules in there for all kinds of animal tissues.
Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
tjj <@t> stowers-institute.org
------------------------------
Message: 5
Date: Thu, 16 Jun 2005 10:05:24 -0500
From: Kathleen Spencer <kspencer <@t> utmem.edu>
Subject: Re: [Histonet] Cryostat sections with small bubbles
To: Alan Bright <abright <@t> brightinstruments.com>
Cc: histonet <@t> lists.utsouthwestern.edu, Katri Tuomala
<katri <@t> cogeco.ca>, Emily Jane Wiesner-Camm
<Emily.Wiesner <@t> medecine.unige.ch>
Message-ID: <0ff76615670407b429351b22aca29125 <@t> utmem.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Yes Alan, but when I put my finger on the block face to warm it, there
is a kimwipe or gauze between my finger and the tissue. It works
really well, so I guess the Ark is still around.
As the owner of a company that sells cryostats, you need to be aware
that not everyone has or can obtain such devices.
Many of us, especially in research, work with old and crippled
equipment, thus we need all the help we can get from our fellow
technicians.
Kathleen Spencer, HT ASCP
Lab Manager/LCM Supervisor
UTHSC
On Jun 16, 2005, at 5:11 AM, Alan Bright wrote:
> Emily,
>
> Basically you need to section brain at -8 to -12:C as colder
> temperatures will cause what you are getting plus cracking. However to
> achieve good quality sections at these temperatures you will need to
> maintain the knife and anti-roll temperatures at -20:C or lower to
> allow the section to slide in between the knife and the anti-roll
> plate unimpeded. Which is what Kathleen is doing when she warms the
> block face with her finger, a technique that went out with the Ark and
> should not be used as firstly you could have an infection issue and
> secondly it is not a very scientific approach in this day and age when
> there are devices fitted to some cryostats such as independent
> specimen temperature control to perform this task with full and
> repeatable control and of most importance, ease.
>
> Best Regards
>
> Alan Bright
>
> Bright Instrument Co.Ltd.
> St Margaret's Way
> Huntingdon
> Cambridgeshire
> PE29 6EU
> England
>
> Tel No:+44 (0)1480 454528
> Fax No:+44 (0)1480 456031
> Email: abright <@t> brightinstruments.com
> Web Site: www.brightinstruments.com
>
>
>
> -----Original Message-----
> From: Kathleen Spencer [mailto:kspencer <@t> utmem.edu]
> Sent: 15 June 2005 16:50
> To: Katri Tuomala
> Cc: histonet <@t> lists.utsouthwestern.edu; Emily Jane Wiesner-Camm
> Subject: Re: [Histonet] Cryostat sections with small bubbles
>
>
> Hi Emily,
>
> I cut rat brain sections at -18 to -20 and often have to warm the block
> face with my finger before each section. I also have better luck if I
> cut sections at 10u. These are fresh frozen brains. If I cut them at
> 20u I get bubbles under the sections. I keep them in the cryostat, and
> then fix the sections in cold fix, buffer wash, water wash, then air
> dry them in the hood.
> When I cut rat brain that has been perfused, I cut 20u floating
> sections. They never adhere well to slides, even if I use a hot plate.
> I always have bubbles under the section. That is why in this case we
> use floating sections.
>
> I hope this is helpful.
>
> Kathleen Spencer
>
>
> On Jun 14, 2005, at 8:39 PM, Katri Tuomala wrote:
>
>> Hi Emily,
>> I have never heard of hot plating cryostat sections. You may be
>> boiling the moisture under the section forming bubbles or do you air
>> dry them well first? Also -35 C seems too cold for brain sections, you
>> may be getting some cutting artifacts.
>> I'm sure there'll be others with helpful information for you.
>> Katri
>>
>> Katri Tuomala
>> Hamilton, Ontario, Canada
>> ----- Original Message ----- From: "Emily Jane Wiesner-Camm"
>> <Emily.Wiesner <@t> medecine.unige.ch>
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Tuesday, June 14, 2005 10:36 AM
>> Subject: [Histonet] Cryostat sections with small bubbles
>>
>>
>>> Hi All,
>>> I was wondering whether anyone can let me know why small bubbles
>>> appear underneath my rat brain sections when I cut them on a cryostat
>>> (at -35) when they appear perfectly flat before placing them on a
>>> hotplate. How they can be avoided?
>>> Thanks,
>>> Emily
>>>
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _______________________________________________
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>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
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------------------------------
Message: 6
Date: Thu, 16 Jun 2005 16:31:22 +0100
From: andrew.macduff <@t> ed.ac.uk
Subject: [Histonet] Negatives for immunos using polyclonal antibody
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1118935882.42b19b4a89a49 <@t> staffmail.ed.ac.uk>
Content-Type: text/plain; charset=ISO-8859-15
Dear all
A quick question-
when doing IHC with a polyclonal Ab is a serum negative sufficient since
there
can be no isotype specific control Ab?
Thanks for the help
Andrew
Andrew MacDuff
Clinical Research Fellow
MRC Centre for Inflammation Research
Medical School
Edinburgh University
------------------------------
Message: 7
Date: Thu, 16 Jun 2005 11:53:56 -0400
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] Schiff's reagent in microwave?
To: "'Jacquie.Farnsworth <@t> cls.ab.ca'" <Jacquie.Farnsworth <@t> cls.ab.ca>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB42CD <@t> fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1
We did this microwaving of the Schiff reagent for awhile, but the step is
only 15 minutes at room temperature so it wasn't worth using the microwave
for this application. The Schiff Reagent last longer, too!! -Janet
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Jacquie.Farnsworth <@t> cls.ab.ca
Sent: Thursday, June 16, 2005 10:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Schiff's reagent in microwave?
Hi Histonetters,
Does anyone have some thoughts on microwaving Schiff's reagent? We
currently microwave Schiff's in our plastics laboratory (for PAS stain on
our renal biopsies). We take the heated reagent out of the microwave, and
place it in a fumehood immediately and remove slides. One of our techs is
still having problems with the fumes. R95 masks are available, but I am
wondering if anyone has any thoughts? Suggestions? Does anyone else
microwave Schiff's reagent?
Thank you in advance,
Jacquie
Jacqueline Farnsworth
Anatomic Pathology Tech III
Calgary Laboratory Services
Jacquie.Farnsworth <@t> CLS.ab.ca
PH: (403) 944-1578
Pager: 212-8223 #1933
CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain
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disclose, copy, retain, distribute, use or modify any of the contents of
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------------------------------
Message: 8
Date: Thu, 16 Jun 2005 16:49:39 +0100
From: Stephen.Eyres <@t> sanofi-aventis.com
Subject: [Histonet] Microwave use in Pharmaceutical/CRO histo labs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF8E68B798.682FBF31-ON80257022.0056B283 <@t> sanofi-aventis.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi All,
Does anyone working in pharmaceutical/CRO's have any experience using
microwave tissue processing? I'd be interested in your experiences,
especially bad ones!!
Cheers
Steve
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------------------------------
Message: 9
Date: Thu, 16 Jun 2005 11:04:04 -0500
From: "Dianne Holmes" <dholmes <@t> anatomy.umsmed.edu>
Subject: [Histonet] Required CEU's
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s2b15caf.039 <@t> GWIA1.umsmed.edu>
Content-Type: text/plain; charset=US-ASCII
I have heard that there is a required number of CEU's for HT's to get
within each year to maintain their certification. Is this so and if
it is - how many?
------------------------------
Message: 10
Date: Thu, 16 Jun 2005 11:15:06 -0500
From: "Nita Searcy" <NSEARCY <@t> swmail.sw.org>
Subject: [Histonet] Muscle Referrals @ Baylor - Houston
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s2b15f47.085 <@t> swmail.sw.org>
Content-Type: text/plain; charset="us-ascii"
Does anyone know the contact person for the above?
Thanks
Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Temple, Texas
Division Manager, Anatomic Pathology
254-724-2438
-------------- next part --------------
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Nita Searcy
TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:NSEARCY <@t> swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
END:VCARD
------------------------------
Message: 11
Date: Thu, 16 Jun 2005 12:01:24 -0400
From: "Vinnie Della Speranza" <dellav <@t> musc.edu>
Subject: [Histonet] One of our own in need ! Please Read
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: carrie <@t> nsh.org, bali02 <@t> cox.net
Message-ID: <s2b16a18.030 <@t> cl.musc.edu>
Content-Type: text/plain; charset=US-ASCII
I am taking the liberty of sharing a correspondence I've had with a fellow
Histotech who is facing some very difficult circumstances involving her
baby's serious illness. I know that histotechs have big hearts and I would
just like to make it possible for you to be aware of Lisa's circumstances. I
am also hopeful that some of you may be able to offer her additional advice
on where she might obtain financial support.
you will need to scroll to the very bottom to see Lisa's first
correspondence with me.
I apologize in advance to anyone who may object to what may appear as
fundraising on the list. I am simply making Lisa's circumstances known to
you on her behalf.
also, please forgive the lengthy message. her original message to me had a
photo of her child attached but I know the list does not allow for
attachments so it has been removed. I imagine that you can obtain the photo
if you wish directly from Lisa. I have removed her home phone number from
her original message at her request.
It is circumstances like hers that remind me of what is most important in
life . I'm sure that your prayers and good wishes would be most welcomed
sincerely
Vinnie Della Speranza
>>> "lisa barbarisi" <bali02 <@t> cox.net> 06/16/05 01:56AM >>>
Hello The address that I gave you for the Funds for Lisa was incomplete I
corrected it below.
Thanks again,
Lisa
----- Original Message -----
From: "lisa barbarisi" <bali02 <@t> cox.net>
To: "Vinnie Della Speranza" <dellav <@t> musc.edu>
Sent: Wednesday, June 15, 2005 9:54 PM
Subject: Re: Fw: Fund raising at the national convention
> Dear Vinnie,
>
> Thank you for your response. I did not think of posting on the Histonet.
> It sounds like a good idea. I would however like to keep my home phone
> number out of the letter and please do add the web site
> www.alysa-anne.com it is now up and is just wonderful. I was told that
> it is still being worked on as they are going to add a guest book for
> people to sign in on. Also, I would appreciate it if you mentioned the
> "Funds for Lisa" account #44699 any donations may be sent to:
>
> Funds for Lisa Account 44699
> Research Administration
> Mayo Clinic Scottsdale
13400 East Shea Blvd.
> Scottsdale, AZ 85259
>
> All donations are tax deductible and a donation receipt will be sent as
> long as the name and address are included either on the check or the
> envelope.
>
> I do appreciate all of the information that you sent there are some that I
> have not called and I will do so. It is so hard to take the time needed to
> research and call people so it is wonderful when others offer a hand.
> Thank you for your wonderful suggestion.
>
> Please forward what you post on the Histonet and the link. I have trouble
> keeping track of things so having them in a e-mail helps me keep track. I
> appreciate your help with this.
>
> Thank you so much for your help I look forward to hearing from you again.
>
> Lisa & Alysa Anne
>
>
> ----- Original Message -----
> From: "Vinnie Della Speranza" <dellav <@t> musc.edu>
> To: <rstaskiewicz <@t> agr.state.il.us>; <ski3247 <@t> aol.com>;
> <gideon <@t> battelle.org>; <bali02 <@t> cox.net>; <jburrill <@t> criver.com>;
> <janet.tunnicliffe <@t> fraserhealth.ca>; <rose.clarke <@t> fraserhealth.ca>;
> <lenaspencer <@t> insightbb.com>; <mjones4 <@t> isuhsc.edu>; <jalvare <@t> jhmi.edu>;
> <mmckown <@t> kih.net>; <patpatterson <@t> mhd.com>; <pruegg <@t> msn.com>;
> <kzeitner <@t> nebraskamed.com>; <ssumida <@t> whidbey.com>
> Cc: <mbarbarisi <@t> cox.net>; <carrie <@t> nsh.org>
> Sent: Monday, June 13, 2005 12:41 PM
> Subject: Re: Fw: Fund raising at the national convention
>
>
> Dear Lisa,
> I cannot tell you how sad I am to learn of your circumstances and of
> course your baby's illness. I have no doubt that this is a very difficult
> time for you and I imagine that many could not begin to imagine the
> challenges you are facing as a result. I know that you have been a member
> of NSH for the last few years and everyone understands your lack of recent
> participation. I will pray that yours and Alysa's circumstances begin to
> improve.
>
> to be quite frank, I am struggling with figuring out how we might help
> you. I would probably have to consult with the society's attorney due to
> our non-profit status. fund raising of any sort could create unforeseen
> problems for the society. given this concern, I don't believe that the
> society could sanction or conduct formal fundraising on your behalf.
>
> I do not know if you have considered placing your letter on Histonet. I
> would be happy to do this for you if you indicate that you would like me
> to do so. The Histonet list reaches histotechs from around the globe and
> is an independent group not constrained by tax and corporate laws. I don't
> speak for that group but I've found that histotechs have big hearts and
> at the least someone more knowledgeable than I may be a source of valuable
> information that might ease your circumstances. I hope you will consider
> this avenue and as I've said, I would be happy to post your letter to me
> on that list.
>
> our Executive Director, Carrie Diamond, has pulled some information from
> the American Cancer Society website that addresses avenues for financial
> assistance for circumstances like yours. I apologize if you are already
> aware of this information but here it is:
>
> from American Cancer Society website.
> Where can families get help financially?
>
> Most families find it difficult to turn to others or to agencies and
> funds for financial help. Families generally take pride in being
> self-sufficient and providing for their own needs. The extra expenses of
> a child's cancer may be for the first time with problems with money.
> They should remember that their problems in such a situation are
> temporary and not unique. In the future, they may be the ones in a
> position to offer financial help to others.
>
> There are several possible sources of help for families in financial need:
>
> * income assistance for low-income families through Supplemental
> Security Income (SSI) benefits
> * income assistance for needy families from the Aid to Families with
> Dependent Children (AFDC) program
> * help with travel, meals and lodging from public and private programs
> * assistance with basic living costs (rent, mortgage, insurance
> premiums, utilities, telephone) from public and private programs
> * help from church, civic, social, and fraternal groups in the
> community
> * general help from special funds in the medical center or community
> * assistance from targeted fundraising for an individual patient or
> family.
>
> Organizations
>
> American Cancer Society, Inc. 800-227-2345
> Candlelighters Childhood Cancer Foundation 800-366-2223
> Corporate Angel Network, Inc. 866-328-1313
> National Children's Cancer Society 800-532-6459
> National Marrow Donor Program 800-627-7692
> National Patient Air Transport Hotline 800-296-1217
> Ronald McDonald House Charities 630-623-7048
> The Leukemia & Lymphoma Society 800-955-4572
>
> Organizations (Government):
>
> U.S. Department of Labor (COBRA information)
> Pension and Welfare Benefits Administration 800-998-7542 (Publications
> only) 202-219-8776
> Americans with Disabilities Act, Civil Right Division, Disability Rights
> Section 800-514-0301
> Hill-Burton Program 800-638-0742
> Internal Revenue Service (publications) 800-829-3676 800-829-1040
> (Assistance and Information)
> National Cancer Institute 800-422-6237
> Social Security Administration (SSI information) 800-772-1213
>
> Other Resources:
>
> American Brain Tumor Association 800-886-2282
> American Kidney Fund 800-638-8299
> Bone Marrow Transplant Information Network 888-597-7674
> Brain Tumor Foundation for Children, Inc. 770-458-5554
> Children's Organ Transplant Association, Inc. 800-366-2682
> Cancervive 310-203-9232
> Curesearch National Childhood Cancer Foundation 800-458-6223
> Lymphoma Research Foundation of America 800-500-9976
> National Association of Hospital Hospitality Houses, Inc. 800-542-9730
> National Association of Personal Financial Advisors 800-366-2732
> National Bone Marrow Transplant Link 800-546-5268
> National Brain Tumor Foundation 800-934-2873
> National Children's Cancer Society 800-532-6459
> National Coalition for Cancer Survivorship 877-622-7937
> National Foundation for Credit Counseling 800-388-2227
> National Information Center for Children and
> Youth with Disabilities 800-695-0285
> National Insurance Consumer Helpline 800-942-4242
> National Organization for Rare Disorders, Inc. 800-999-6673
> National Self-Help Clearinghouse 212-817-1822
> National Transplant Assistance Fund 800-642-8399
> Oley Foundation, Inc. (nutritional information) 800-776-6539
> Patient Advocate Foundation 800-532-5274
>
> Other Organizations (Government):
>
> National Cancer Institute (NCI)
> Cancer Information Service 800-422-6237
> Centers for Disease Control and Prevention 800-311-3435
> Department of Health and Human Services 877-696-6775
> Medicare Hotline 800-633-4227
> National Health Information Center 800-336-4797
> Veterans Benefits Administration 800-827-1000
> U.S. Department of Veterans Affairs
>
> I hope that you will let us know when the website you are planning is up
> and running so it will be easier for people to offer donations.
>
> God bless you both.
> sincerely
> Vinnie Della Speranza
> President, National Society for Histotechnology
>
>
>>>> "lisa barbarisi" <bali02 <@t> cox.net> 06/10/05 02:37AM >>>
> Please read. I was not sure who to send this to so I tried to include
> everyone.
>
> Thank you in advance for your time. Prayers are very welcome also.
>
> Lisa A. Barbarisi HT(ASCP)
>
> ----- Original Message -----
> From: lisa barbarisi
> To: histo <@t> nsh.org
> Cc: bali02 <@t> cox.net
> Sent: Thursday, June 09, 2005 11:17 PM
> Subject: Fund raising at the national convention
>
>
> Hello All,
>
> I am writing to ask for help in the form of a fundraiser at the national
> convention. I am an HT( ASCP) certified Histotech who has worked in the
> field of histotechnology for over eighteen years. I worked as the
> coordinator of the histology research facility at the Mayo Clinic I am
> currently on unpaid leave.
>
> After almost three years of fertility treatments and
> invitro-fertilizations I spent all of my savings and took out a second
> mortgage on my home and I was finally blessed to become pregnant. I gave
> birth to a beautiful baby girl on August 30th 2004.
>
> I am a single first time mom with one income of which is zero at the
> moment since I am on an unpaid leave from my current position at the Mayo
> Clinic research facility as a Histotech. I am looking for financial help.
> My beautiful baby girl Alysa Anne was diagnosed with stage four
> neuroblastoma on March 25th 2004 she was not yet seven months old. She has
> had surgery to try and remove the primary tumor with no luck. She is
> currently undergoing chemotherapy to try and shrink the primary tumor
> which is located on her right adrenal gland and there are tumors present
> in her liver (to many to count), bone marrow, her bones base of her skull,
> chest bone, around both of her eyes and her pathology is unfavorable. I
> have been told by her oncologist that this is going to be a long hard road
> for both my daughter and I. Between the chemotherapy there are doctors
> visits twice and sometimes three times a week to check her progress and
> also her blood counts. She is on daily shots of Neupogen and multiple
> medications. How is such a small body going to take so much? I just can't
> believe this is happening.
>
> I'm sorry, I could go on, but you may not be interested in helping me and
> I will understand. I am at the end of my rope. I know that many people may
> ask for help, but I just had to try. My daughter is my life and I will do
> whatever I have to to help her fight this horrible disease . I was unable
> to attend the NSH convention last year in Canada as my daughter had
> finally blessed me with her arrival (she was over a week late and after a
> long hard labor I finally had to have a c-section). I will not be able to
> attend this year either since my daughter is having chemotherapy she just
> made it through her third round. I feel helpless watching the poison being
> pumped inter her little body thru a port in her chest.
>
> I wonder if there is anyone who would like to take on this huge
> undertaking of either a fundraiser or maybe just taking donations to the
> "Funds for Lisa" a not for profit account set up by a co-worker who wanted
> to help me.
>
> If anyone is interested I would be so happy. Please e-mail me at
bali02 <@t> cox.net . There will be a
> website that is not as far as I know up and running it will tell more
> about me and my daughter. It will also have updates on her progress. Once
> I get word that it is up and running I can e-mail the site.
>
> I would appreciate a response even if it is negative.
>
> I have to beat this MONSTER called NEUROBLASTOMA to save my daughter .
>
> Sincerely,
>
> Lisa A. Barbarisi
>
> Picture of Alysa Anne at six months old three weeks before diagnosis is
> attached.
>
> I
>
------------------------------
Message: 12
Date: Thu, 16 Jun 2005 11:26:11 -0500
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] Schiff's reagent in microwave?
To: <Jacquie.Farnsworth <@t> cls.ab.ca>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A61F23E937E4DA488E0C0F60093843D901E4989F <@t> ccetxm030.echristus.net>
Content-Type: text/plain; charset="iso-8859-1"
Do you have room in the fume hood for the microwave?
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533
My opinions are my own and do not reflect those of my employer. Long live
free speech!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Jacquie.Farnsworth <@t> cls.ab.ca
Sent: Thursday, June 16, 2005 9:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Schiff's reagent in microwave?
Hi Histonetters,
Does anyone have some thoughts on microwaving Schiff's reagent? We
currently microwave Schiff's in our plastics laboratory (for PAS stain on
our renal biopsies). We take the heated reagent out of the microwave, and
place it in a fumehood immediately and remove slides. One of our techs is
still having problems with the fumes. R95 masks are available, but I am
wondering if anyone has any thoughts? Suggestions? Does anyone else
microwave Schiff's reagent?
Thank you in advance,
Jacquie
Jacqueline Farnsworth
Anatomic Pathology Tech III
Calgary Laboratory Services
Jacquie.Farnsworth <@t> CLS.ab.ca
PH: (403) 944-1578
Pager: 212-8223 #1933
CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain
confidential, personal and/or privileged information intended for a specific
purpose and recipient. If you are not the intended recipient do not
disclose, copy, retain, distribute, use or modify any of the contents of
this transmission. If you received this transmission in error please notify
me immediately by return e-mail or telephone and destroy the entire
transmission and any copies produced. Thank you.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Thu, 16 Jun 2005 11:45:10 -0500
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] Brain fixative
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A61F23E937E4DA488E0C0F60093843D901E498A0 <@t> ccetxm030.echristus.net>
Content-Type: text/plain; charset="iso-8859-1"
We use 20% formalin, and leave it in there for about a month.
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533
My opinions are my own and do not reflect those of my employer. Long live
free speech!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Thursday, June 16, 2005 9:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Brain fixative
Does anyone fix their brain tissue from autopsies in anything other than
10% buffered formalin? The PA I am working with seems to feel they used
a 4% solution of lightly buffered formalin from Mallincroft. Can anyone
help me with this, as I am only used to using 10% formalin, as we use
for all of our routine histology fixation. Any ideas would be greatly
appreciated and thanks ahead of time to all of my fellow
histonetters..what a great group you are!!
+______________________________________+
________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 16 Jun 2005 17:49:46 +0100
From: "Edmondson David \(RBV\) NHS Christie Tr"
<David.Edmondson <@t> christie-tr.nwest.nhs.uk>
Subject: RE: [Histonet] BCIP/NBT ready-to-use
To: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Cc: "Histonet \(E-mail 2\)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C11617242B6CBF4E87F9E70937893BB439A940 <@t> cht-mail-2k.xchristie.nhs.uk>
Content-Type: text/plain; charset="iso-8859-1"
DAKO do complete reagent in one, and the BCIP/NBT/INT that we use, its all
used within date so can not help with long term stability. No ppt formed
that we have seen
Dave
Christie Hosp
Manchester UK
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Johnson,
Teri
Sent: 15 June 2005 23:22
To: Histonet
Subject: [Histonet] BCIP/NBT ready-to-use
We are currently using a substrate system which has two separate
components for BCIP and NBT, and then have to combine them just before
use. I'm looking into alternate systems and have seen several vendors
who offer what appears to be ready-to-use substrate. Is anybody using
them and can you comment on stability with regard to expiration date,
length of use, and lack of problems after the slides have been stained
(I recall a recent thread regarding horrible overstaining after the
slides had been coverslipped). Thanks muchly!
Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
tjj <@t> stowers-institute.org
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 16 Jun 2005 16:54:47 +0000
From: mucram11 <@t> comcast.net
Subject: RE: [Histonet] Schiff's reagent in microwave?
To: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>,
<Jacquie.Farnsworth <@t> cls.ab.ca>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<061620051654.18290.42B1AED7000002C9000047722200748184CECE030E9D0C9A03 <@t> comc
ast.net>
Content-Type: text/plain
Technically we should all have the oven under the hood and it is not always
possible if we need to use the hood for anything else. I know I was told to
look into the cost of a laboratory microwave and thought the people I worked
for at the time would have heart attack when they saw the quote. I used the
Target MW as close to the hood as I could get it and that allowed me to move
the Schiff's and anything else very quickly under the hood. I could hold my
breathe and get it there. Otherwise I don't what would stop the fumes
completely for you.
In an ideal world we would all have large hoods and lab MWs for safety.
Pam Marcum
-------------- Original message --------------
> Do you have room in the fume hood for the microwave?
>
> Juan C. Gutierrez, HT(ASCP)
> Histology Laboratory Supervisor
> (210)704-2533
>
> My opinions are my own and do not reflect those of my employer. Long live
free
> speech!
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Jacquie.Farnsworth <@t> cls.ab.ca
> Sent: Thursday, June 16, 2005 9:52 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Schiff's reagent in microwave?
>
> Hi Histonetters,
> Does anyone have some thoughts on microwaving Schiff's reagent? We
currently
> microwave Schiff's in our plastics laboratory (for PAS stain on our renal
> biopsies). We take the heated reagent out of the microwave, and place it
in a
> fumehood immediately and remove slides. One of our techs is still having
> problems with the fumes. R95 masks are available, but I am wondering if
anyone
> has any thoughts? Suggestions? Does anyone else microwave Schiff's
reagent?
>
> Thank you in advance,
> Jacquie
>
> Jacqueline Farnsworth
> Anatomic Pathology Tech III
> Calgary Laboratory Services
> Jacquie.Farnsworth <@t> CLS.ab.ca
> PH: (403) 944-1578
> Pager: 212-8223 #1933
>
>
>
>
> CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain
> confidential, personal and/or privileged information intended for a
specific
> purpose and recipient. If you are not the intended recipient do not
disclose,
> copy, retain, distribute, use or modify any of the contents of this
> transmission. If you received this transmission in error please notify me
> immediately by return e-mail or telephone and destroy the entire
transmission
> and any copies produced. Thank you.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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