[Histonet] Help, slide adhesive for wood

[Due, Brice]

Not an adhesive, but along the same lines as the previous post, you could use
what are called "capillary gap" slides. Same idea as using a coverslip. Cap-gap
slides have extra frosted areas at the bottom corners, so when you double them
up they maintain a gap of a known thickness unlike coverslips. Solns get sucked
up into the gap and rinsing would not be so problematic as with coverslips.
There are automated stainers based on cap-gap slides. You can place a cap-gap
slide against a regular charged slide and the gap is roughly half the size. They
cost about twice as much as charged slides, but for this purpose you could reuse
them until the corner frostings get scraped off. To separate two slides, you
must fully immerse the pair. Once submerged they come apart easily -- otherwise
they stick together tightly.

-brice

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bonner,
Janet
Sent: Friday, June 17, 2005 3:21 PM
To: 'Craig Kallsen '; 'histonet-bounces <@t> lists.utsouthwestern.edu ';
'histonet <@t> lists.utsouthwestern.edu '
Subject: RE: [Histonet] Help, slide adhesive for wood


 You could stain these sections by coverslipping with water and using an
absorbant towel to exchange solutions (stains/dyes)by absorption and
effusion. The slide could be cleared this way, but not very well.  Try
putting the fluids on the slide, let sit, and tip onto the towel to remove
the solutions by osmosis.   

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Sent: 6/17/2005 2:14 PM
Subject: [Histonet] Help,  slide adhesive for wood

I am having trouble getting wood specimens embedded in paraplast plus to

stick to glass slides.  The bark and phloem tissues stick fine, but the 
wood made up of the xylem does not.  I have tried charges slides and a 
gelatin adhesive in the water bath.  I am using ethanol based 
Safranin-O, Fast Green, Orange G and Iodine stains.  Does anyone have an

adhesive suggestion?

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