[Histonet] Brain tissue with GFP: fixing or not

[Ajai Vyas]

Greetings!
I am trying to visualize some GFP +ve neurons in  mouse. I have tried
fresh freezing these by embedding in OCT and putting in slurry of
alcohol+dry ice. I have problem with curling and tissue integrity when
I section these brains in cryostat (-15 and -20 degree C). For the
next lot I was wondering if I should attempt to
1) perfuse animals with fresh PFA, cryoprotect brains with sucrose and
section or
2) try other methods of snap freezing suggested on histonet archives
and/or try to play more with cryostat temperature
Any suggestion and pointers will be greatly appreciated,
Ajai