[Histonet] Cryostat sections with small bubbles

[cindipqr <@t> wowway.com]

I agree, -35 seems way too cold.  I cut rat brains around -18


---------- Original Message -----------
From: "Katri Tuomala" <katri <@t> cogeco.ca>
To: "Emily Jane Wiesner-Camm" <Emily.Wiesner <@t> medecine.unige.ch>, 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, 14 Jun 2005 21:39:51 -0400
Subject: Re: [Histonet] Cryostat sections with small bubbles

> Hi Emily,
> I have never heard of hot plating cryostat sections. You may be 
> boiling the moisture under the section forming bubbles or do you air 
> dry them well first? Also -35 C seems too cold for brain sections, 
> you may be getting some cutting artifacts. I'm sure there'll be 
> others with helpful information for you. Katri
> 
> Katri Tuomala
> Hamilton, Ontario, Canada
> ----- Original Message ----- 
> From: "Emily Jane Wiesner-Camm" <Emily.Wiesner <@t> medecine.unige.ch>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Tuesday, June 14, 2005 10:36 AM
> Subject: [Histonet] Cryostat sections with small bubbles
> 
> > Hi All,
> > I was wondering whether anyone can let me know why small bubbles appear 
> > underneath my rat brain sections when I cut them on a cryostat  (at -35) 
> > when they appear perfectly flat before placing them on a hotplate. How 
> > they can be avoided?
> > Thanks,
> > Emily
> >
> >
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------- End of Original Message -------