[Histonet] iron hematoxylins

[Bryan Llewellyn]

I have blued Weigert's iron hematoxylin in the past, although I didn't do it 
as a routine.  I wondered if the acid in the Weigert's solution affected the 
colour and I tried it to find out.  Blueing does deepen the colour, although 
not as significantly as with alum hematoxylin.  However, the acid solutions 
I used afterwards in Masson's trichrome undid it and the end result was the 
same.  It is useful if the iron hematoxylin is used alone without a 
counterstain, perhaps on smears for amoebae, but otherwise not.  Other iron 
hematoxylin stains of the Heidenhain type do not need blueing.  The mordant 
does that.

Bryan Llewellyn



----- Original Message ----- 
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
To: "'Steven Coakley'" <sjchtascp <@t> yahoo.com>; 
<Histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, June 14, 2005 5:07 PM
Subject: RE: [Histonet] iron hematoxylins


> I've never blued after a Weigert iron hematoxylin stain. Nuclei are either
> black, or they aren't. If they are not, then the iron hematoxylin is old
> (more than a couple days old), and putting the tissue into an alkali
> solution won't bring the color back.
>
> I'm curious, why are you bluing? What is the purpose? Maybe then I can 
> help.
>
> Peggy Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven
> Coakley
> Sent: Tuesday, June 14, 2005 10:49 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] iron hematoxylins
>
> Does anyone know if using a bluing agent after weigerts iron hematoxylin
> will achieve the same results a lengthy tap water rince?
>
> Steve
>
>
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