[Histonet] Controls used for Muscle biopsies

[cfockler <@t> mail1.vcu.edu]

-------------------
> Hi Julia, 
> The lab where I did muscle biopsy stains never used controls, but
you really should. There are a bunch of techs on the histonet that
helped me when I needed information so I'm sure they'll see your
question and help you too! I'd like to learn what to use as controls
as well. Thanks for asking!
>  
> Jessica Piche-Grocki, HT
> 
> Celebre Julia <celebrej <@t> HHSC.CA> wrote: 
> Hello Histonetters...
> This is a question for all those labs out there that do enzyme
> histochemistry on muscle biopsies.
> Does anyone run controls along with there test muscle biopsies? If
your
> honest answer is yes (not like mine was), what do you use? 
> 
> Julia Celebre MLT
> Anatomic Pathology
> Hamilton General Hospital
> 905-527-0271 ext46145
> email: celebrej <@t> hhsc.ca
> 
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> We have experimented using controls. We get a lot of research, and
have used fresh rat muscle (quad muscle) and the controls have worked
beautifully.  The only problem is the tissue must be fresh and you can
only store cut controls for so long (in a -80 deep freezer) so it can
be difficult to keep up with.  
Candyce Fockler
Histotechnician
Anatomic Pathology