[Histonet] melanin renoval

[Jackie M O'Connor]

Or how about a red or purple DAB replacement?  Biocare has a couple of 
permanent chromogens that can be used for HRP.  I plan to do exactly this 
for some melanin-loaded xenografts.

Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL
Jackie.OConnor <@t> abbott.com




"Elizabeth Chlipala" <liz <@t> premierlab.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/13/2005 02:58 PM

 
        To:     "'Jane Moose'" <jane.moose <@t> newberryhospital.org>, 
<Histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        RE: [Histonet] melanin renoval


Jane

Rather than perform a melanin bleach why don't you switch to a alkaline
phosphatase detection system and use fast red as your chromagen.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jane
Moose
Sent: Monday, June 13, 2005 2:53 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] melanin renoval


Does anyone have a procedure to bleach unstained slides (from FFPE
tissue) in order to remove melanin
in order to stain with DAB for  PECAM?




Jane Moose
LIS Coordinator/Histology
Newberry County Memorial Hospital
Newberry,  SC
803-405-7129

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