[Histonet] Trouble Adhering Post-Fixed Frozen Cartilage to Slides...

[Geoff McAuliffe]

    Many years ago (1985?) there was a paper in Stain Technology, now 
known as Biotechnique and Histochemistry, called "hot melt corner point 
method" in which the authors used hot melt glue to hold down sections of 
vertebral bodies (or intervertebral disks). The glue was applied at the 
corners of the sections but I think the sections were embedded in 
celloidin. Also, you might want to try staining the sections 
free-floating in watch glasses (or a similar shallow container) and only 
mounting the sections when staining is finished.

Geoff

tsai0058 wrote:

>Hello, 
>
>   I'm trying to adhere frozen intervertebral discs to slides for H&E
>staining as well as immunohistochemistry, and am having a hard time getting
>the tissues to stick to the slides throughout the process.  The tissues
>were obtained fresh, then fixed for 24 hours in Lanas solution (4%
>paraformaldehyde, 14% picric acid in phosphate buffer solution), and then
>stored in a cryopreservative for approximately 1 week, after which they
>were quick frozen in liquid nitrogen.  Does anyone have any protocols that
>they use for post-fixed frozen cartilage?
>
>   Also, I have a colleague who is working with paraffin embedded Temporal
>Mandibular Discs (connective tissue, synomium, muscle, cartilage), and was
>trying to do immunohistochemistry, and he was having some difficulty
>getting the tissue to stick to the slides as well.  Does anyone have any
>thoughts regarding this problem?
>
>   Thanks for your input!
>
>Phil Tsai
>University of Minnesota
>  
>
>
>
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>


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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