[Histonet] Mercurochrome in IBF--replacement?

[Luck, Greg D.]

Jacquie,

We use the orange dye that comes with the set of Davidson's tissue dyes.  To
set the ink better we soak our small biopsies briefly (after we wrap them in
lens paper) in Bouin's solution. Although this is a bit messy it is very
effective, has improved the visualizing for both embedding and cutting and
the pathologists are happy as well.  Good luck,  Greg

Greg Luck, BS, HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Medical Center
800 W. 5th Ave
Spokane, WA 99204
Phone 509.473.7077
Fax 509.473.7133
luckg <@t> empirehealth.org
www.deaconessmedicalcenter.org


-----Original Message-----
From: Jacquie.Farnsworth <@t> cls.ab.ca [mailto:Jacquie.Farnsworth <@t> cls.ab.ca] 
Sent: Thursday, June 09, 2005 10:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mercurochrome in IBF--replacement? 

I've read many articles in the archives about replacing mercurochrome with
eosin, etc.  We are looking for a dye solution to use in IBF fixative for
our prostate biopsies.  (In order to see the tissue easier at embedding).
We are currently trying Mrs. Stewart's bluing solution. With this, the
pathologists have noticed a blue halo around the sections. Generally it does
not interfere with diagnosis, but am wondering if there's something better
out there?

Thank you in advance,
Jacquie

Jacqueline Farnsworth
Anatomic Pathology Tech III
Calgary Laboratory Services
Jacquie.Farnsworth <@t> CLS.ab.ca
PH: (403) 944-1578
Pager: 212-8223 #1933




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