[Histonet] Sialidase digestion

[Geoff McAuliffe]

Hi Laurie:

    I have not done CI with sialidase but I have used hyaluronaidase 
many years ago. Keep in mind that there are two kinds of hyaluronidase, 
Bovine testicular and Streptococcyl, I think the latter is more 
specific. You need a book on mucin/connective tissue histochemistry, a 
lot has been done using gut mucins as models but I forget the details. 
See a histochemistry text for the correct path to follow.

Geoff

Laurie Colbert wrote:

>Has anyone ever done (or heard of) a colloidal iron stain with sialidase digestion?  Our pathologists have a mucinous skin tumor and are trying to differentiate between a primary breast CA and a primary eyelid tumor.  It may be run in conjunction with hyaluronidase digestion.  Any references or procedures??
>
>Laurie Colbert
>Huntington Hospital
>(626) 397-8620  
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
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mcauliff <@t> umdnj.edu
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