[Histonet] Adipocyte staining... ORO Churukian Style...

[Due, Brice]

Hello Atoska, your protocol should explain the mixing. I use Churukian's ORO as
printed in Bancroft 5th ed. The dextrin is a stabilizer of some sort, not a
differentiator. The procedure is one step plus counterstain. For Churukian's
procedure, the working solution is a combination of the ORO-isopropanol and the
aqueous dextrin, so the literal answer to your questions is: mix the dextrin in
water, then combine before use. Filtering or letting stand may be needed
depending on which variant of the procedure you are following. In the variant I
use (p. 208 in Bancroft 5ed.) the solutions are mixed and then remain stable for
years -- until they stop working. I use this every week for muscle bxs and it
never fails and is never fickle. 

-brice
Neuropathology Lab
Brigham & Women's Hospital
Boston

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Atoska S.
Gentry
Sent: Wednesday, June 08, 2005 12:20 PM
To: Histonet
Subject: [Histonet] Adipocyte staining


Hello, please is anyone out there familiar with the Oil Red O protocol for 
adipocytes which requires differentiation in 1% Dextrin? A colleague of 
mine needs to know if the Dextrin has to made in H2O or Isopropanol ? The 
Oil Red O is made in Isopropanol. Your prompt replies will be much 
appreciated. Atoska


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