[Histonet] goldners trichrome

[Elizabeth Chlipala]

Hello Everyone
 
I have to perform a goldners trichrome.  The two protocols that I have
do not use bouins as a mordant.  One does state that the trichrome stain
benefits from picric acid fixation but also states that 10% NBF is fine.
Is this correct or should I mordant in bouins prior to staining.  I
figured I would ask the histonet first before I run the stain with and
without using bouins as a mordant.  Any advice would be helpful.
 
Thanks in advance.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
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