[Histonet] Processing with vacuum and/or pressure

[Karen Percival]

Jacqui,

The best process I found for fatty tissues such as breast was to add an
additional absolute alcohol step and to increase the times on each (3 in
total).  The breast tissues process and cut wonderfully, and we rarely
have unfixed tissues or tissues fallilng off of the slides even for IHC
with HIER.

Karen

Karen Percival
Research Scientist I
Wyeth Research
1 Burtt Road
G3025
Andover, MA 01810
888-577-1500 x 4058
kpercival @wyeth.com

>>> Malam Jacqueline <Jacqueline.Malam <@t> rli.mbht.nhs.uk> 6/7/2005
3:38:52 AM >>>
Would tissue processor users please let me know whether it is believed
best
to use vacuum only or both vacuum and pressure when processing,
especially
with blocks of fatty tissues eg breast. We use two Shandon Pathcentres.
I
was recommended to use vacuum only but we do get breast blocks which
still
aren't processed in the centre despite using heat with the processing
reagents.   
Thanks
Jacqui Malam
Royal Lancaster Infirmary
England





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