[Histonet] Tissue microarrays use?. .

[Luis Chiriboga]

Katri, your correct, "sampling" is one of the major issues of the technique.
Most importantly is to design the microarray such that you have multiple
cores from different areas of the same block.  Design and adequate sampling
are the key see the following references:

Nocito, A. et al 2001: Microarrays of bladder cancer tissue are highly
representative of proliferation index and histological grade. J Pathol
194(3) 349-57

Rubin, M.A. et al 2002: Tissue microarray sampling strategy for prostate
cancer biomarker analysis. Am J Surg Path 26(3) 312-9

we routinely put 4 cores/block, and if indicated we have put as many as 8
from the same block. Even if the requires creating additional blocks, you
still get the cost benefit from the reduced volume of samples...
LC>>>

____________________________________
Luis Chiriboga Ph.D.
NYU Cancer Institute and
Bellevue Hospital Center
New York University School Of Medicine
Department Of Pathology 4W27
462 First Avenue
New York, N.Y. 10016
W(212) 562-4667.
F(212) 263-2041






-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Henry,
Charlene
Sent: Monday, June 06, 2005 11:22 AM
To: Katri Tuomala; Madary, Joseph; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Tissue microarrays use?. .


Our pathologist review H&E slides of each block to be used in a tissue
array and they determine which blocks are grouped together for each
tissue array block. They also mark on the H&E slide the exact area they
want punched.

Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100


-----Original Message-----
From: Katri Tuomala [mailto:katri <@t> cogeco.ca]
Sent: Friday, June 03, 2005 5:12 PM
To: Henry, Charlene; Madary, Joseph; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue microarrays use?. .


Hi Charlene,
Knowing that so many tumors can give a very heterogenous staining
patterns
( some areas strongly positive and others weak or even negative ), how
do
you overcome this problem with microarrays? I would have a hard time
trusting the results with such small samples.
Katri

Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message -----
From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
To: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 03, 2005 4:43 PM
Subject: RE: [Histonet] Tissue microarrays use?. .


They are greatly instrumental in research. Working at a research
facility, we were able to pay for the purchase of the instrument with
only 1 research project. Example: A pathologist has a research project
that he/she is doing say on Neuroblastoma. They have 150 blocks that
they need a total of 10 IHC tests on each block. You take the 150 blocks
and prepare 2 tissue micro array blocks and then run your 10 IHC test on
the 2 TMA blocks. You have saved a great deal of money because you have
20 IHC tests instead of 1500 IHC tests that would have been needed
without the tissue array blocks. At approximately $15 for each IHC test,
you can see that $300 is much better than $22,500.

Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madary,
Joseph
Sent: Friday, June 03, 2005 3:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tissue microarrays use?. .


Can someone explain to me what the big deal is on tissue microarrays?  I
understand that they are many perfect circles of known diseased or
normal tissues than can be used as a control for various applications.
What are the applications for this stuff on routine or research that
would make it worth it for us to either do it ourselves or contract it
out?  Can it be done in house cheap?

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