[Histonet] LFB with Vector ABC kits

[Elizabeth Chlipala]

Nick

I have done Immunohistochemical staining with DAB as a chromagen and
then performed an acid fast stain on top of it.  It worked just fine. If
you want to see an image there is on our web page.  I would not think
that staining with LFB after the immuno would be a problem as long as
you use DAB for a chromagen.  There is a nice article in Histologic (I
don't know what issue, maybe Vinnie will know) which demonstrated a
method for immunohistochemistry and PAS staining in kidney sections.
You just need to go the sakurausa.com and search the older issues of
that publication.

Liz   

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Donin,
Nick (NIH/NCI)
Sent: Monday, June 06, 2005 1:19 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] LFB with Vector ABC kits

I am trying to stain using LFB in combination with the Vector ABC
Staining
kits.  For the Vector Kit staining, I am using a biotin-conjugated
secondary
antibody, which then conjugates with an avidin-peroxidase molecule which
reacts with a DAB substrate.  Does anyone know if these two methods are
compatible?  If so, is it preferable to do either one first?
Additionally,
my lab has run out of Lithium Carbonate for the LFB protocol, can I
substitute with Lithium Chloride?  I'm trying to stain formalin fixed,
paraffin embedded mouse brain.  Thanks for your help.  Email me any
questions or suggestions you have.  Thanks.   

 

Nick Donin

CRTA

Neuro-Oncology Branch

National Cancer Institute

National Institutes of Health

9000 Rockville Pike

Building 35, Room 2B-203

Bethesda, MD 20892

 

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