[Histonet] question on stains for plant histology

[Andrea Grantham]

Good Monday morning!
I'm asked to do several stains on plant tissue this week and I have the 
protocols from Ruzin's book but have a few questions.
A Safranin and Hematoxylin stain calls for differentiation in acidified 50% 
ETOH. Can somebody tell me what acid do you use to do this and do you just 
pH until the ETOH is acidic? The stain also calls for slightly acidified DI 
(doesn't say but I'm assuming DI water) to wash the slides after staining 
in Hematoxylin. Again - do I just add a few drops of acid - like glacial 
acetic to the DI water?
Last - if anybody has ever done Flemming's Triple Stain can you contact me?
Thanks.
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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