[Histonet] Ventana Benchmark cytology protocols

[Malam Jacqueline]

The few cytology preparations (FNAs and wet preps) we have done here have
been stained using the "non-paraffin, wet slide load" protocol format on our
Benchmark XT, and we chose the best temperature and incubation time for each
antisera tested. 
The FNA slides and preps were previously fixed with our routine cytology
spray fix. As no formalin fixation is used, no antigen retrieval is
necessary. The antisera dilution may have to be altered from that used for
wax sections (if you do them) - you'll just have to see.  
You may have the luxury of being able to try different fixatives rather than
using the one you use routinely, so you could try choosing ones that are
best suited to the antigen you are trying to detect.
Let me know how you get on!
Jacqui Malam
Royal Lancaster Infirmary
England



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