[Histonet] different antigens need different methods for antigen retrieval?

[pex]

Hello, all,
 
I feel confused for immunofluorescence.
I am doing immunofluorescence in vertebra sections. I use the same protocol for immunostaining, but the results are very different, the one is very nice, but the other is weak, I do not know the reasons. Do different antigens need different methods for antigen retrieval? 
Generally, which methods for antigen retrieval in bone sections are better? (Enzyme digestion? or HIER? or both?)
Any suggestions will be helpful for me!
 
Thank you!
 
 
 

		
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