[Histonet] filtering hematoxylin

[Rogerson Kemlo (ELHT) Pathology]

Interesting question. Do you filter to remove haematoxylin/ mordant
precipitant or cells/ tissue? As a recovering Cytologist I would suggest
you filter at least daily, if not 2x daily, to remove cells/ tissue that
can cause 'carry over'. 

Cells from 'fatty organs' such as breast or small celled undiff., from
my experience can do this damage. It's easier to filter than for the
'sword of Damocles' to hang over the Patient. 

-----Original Message-----
From: Diana McCaig [mailto:dmccaig <@t> ckha.on.ca] 
Sent: 01 June 2005 17:19
To: Histonet (E-mail)
Subject: [Histonet] filtering hematoxylin

How often do you filter commercially prepared Harris Hematoxylin (used
on
autostainer)?

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