[Histonet] Frozen TMA's

[CHRISTIE GOWAN]

Thomas,
Do you build your TMA's in a cryostat or on dry ice. Sometimes this has an 
effect on the tissue interface. Also, we use the cryojane tape system by 
Intrumedics. This allows us to get consistent sections each time. If you 
build your TMA's on dry ice let me know and I will be happy to send you our 
protocol.
Christie Gowan

Thomas Crowell wrote...

>Hello all,
>
>I'm hoping that there are some experts among the histonetters who have
>mastered the technique of creating frozen tissue microarray blocks, and
>would be willing to share some of the details with our laboratory.  We are
>currently using a 2.5mm Harris uni-core to make a 4X5 template, and using
>the same core size to sample tissues - the cores fit nicely into the
>templates, however the interface between the tissue core and the OCT block
>is creating much havoc in trying to obtain reproducible sequential
>cryosections (sections curl up, drag along the blade, compress).  Smearing
>a layer of OCT onto the block surface does help to fill in the interface
>irregularities, but only alleviates the problem for 3- 5 sections.
>
>Any suggestions for improvement would be greatly appreciated!
>
>Tom Crowell
>BiogenIdec
>Cambridge, MA
>
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