[Histonet] mouse endothelial cells in brain

[Corazon D. Bucana]

The only time we got good staining is on frozen sections using anti-mouse 
CD31 from either Pharmingen or Serotec in both immunofluorescence and DAB 
or AEC staining.


At 11:54 AM 5/31/2005 -0400, you wrote:
>Histonetters,
>
>This is my first posting, so I'm excited that someone out there might be
>able to help me.
>
>
>I am trying to stain endothelial cells in brain tumors in formalin fixed
>paraffin embedded mouse tissues.  I have tried several antibodies, and none
>of them seem to work.  I am consistently getting high background and no true
>staining.  I have used the following antibodies:
>
>
>
>BD Pharmigen anti-mouse CD31 (PECAM-1) cat #557355
>
>Santa Cruz CD31 (PECAM-1) cat # sc-1506 (I have seen postings that this
>works, but my protocol didn't produce good results)
>
>Cedarlane mouse CD31 cat#CL8930 AP
>
>Cymbus Biotechnology anti-ms CD31 cat# CBL1337
>
>
>
>The protocol I use is shown below.
>
>
>
>
>
>I'm confident that out of these antibodies, at least one will be able to
>stain the endothelial cells in these tumors, but I think that my protocol
>may be the problem.  Could someone possibly suggest and antibody or protocol
>that would work for me?  Thanks so much for your help, much appreciated.
>
>
>
>Nick Donin
>
>CRTA
>
>Neuro-Oncology Branch
>
>National Cancer Institute
>
>National Institutes of Health
>
>9000 Rockville Pike
>
>Building 35, Room 2B-203
>
>Bethesda, MD 20892
>
>
>
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