[Histonet] CD 31

[Dana Settembre]

Kim,
I use Dako's CD31 at an even more potent dilution than Rena does with
Target Retieval Solution from Dako with a uterus as a positive control. 
Trilogy is good.  You should stick with it and make your dilution more
potent.
Dana Settembre
University Hospital-UMDNJ
Newark, NJ

>>> <renafail <@t> bellsouth.net> 7/22/2005 3:18:24 PM >>>
Hi Kim,
   We use CD31 at 1:50, pretreatment HIER using Citrate buffer
Rena Fail

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim
Allred
Sent: Friday, July 22, 2005 3:15 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] CD 31

I am having a problem getting my CD31 to work.  The protocol was for
an
overnight incubation and pepsin pretreatment.  I couldn't get it to
work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab.  Does anyone have any ideas?  This
seemed
to
work well for a while but once again, I'm having problems with it. 
Any
input would be appreciated.  Thanks in advance!  

 

 

 

Kim Allred 

Dermatopathology Associates

Jackson, MS  

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