[Histonet] RE: Confusing IHC results

[C.M. van der Loos]

   Dusco,

   Patty is completely right about this! I have observed the same problem
   with FFPE kidney sections that were pronase pretreated.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Wed, 20 Jul 2005 16:33:04 -0600
   From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
   Subject: RE: [Histonet] Confusing IHC results
   To: "'Trajkovic, Dusko'" <dusko.trajkovic <@t> pfizer.com>,
   <histonet <@t> lists.utsouthwestern.edu>
   In  my  experience the endogenous biotin in liver cannot be completely
   removed
   even with the most aggressive AB blocking, this is why I do not use an
   avidin/biotin detection system, I use labeled polymer instead.
   Patsy