[Histonet] Bcl10

[Megan Clarke]

Hello Histonetters,
We would like to see if anyone can help us with an antibody optimisation for Bcl10?
We have the Zymed Bcl 10 ,clone 151.
We have tried HIER using citrate ph 6.0,citrate EDTA ph 8.0,and Dako TUR both on the Ventana and the Bond Max.Our dilution ranges have been from a 1 in 50 up to 1 in 2000.
Our slides look "muddy" with distorted morphology.With no specific staining.We are using a reactive tonsil as our control.
If anyone is familiar using this and can offer us some help we would really appreciate it.
Thank you very much
Megan Clarke and Zenobia Haffajee
Immunohistochemisty Lab
HAPS>JHH
Newcastle
AUSTRALIA