[Histonet] RE: endogenous peroxidase blues

[C.M. van der Loos]

   Hi all,

   Before everybody is trying to solve their problem of endogenous
   peroxidase activity with Dual Enzyme Blocking Reagent as Veronique
   wrote us, I would like to make a comment. This stuff works great
   indeed in many cases, that's true. But I also found some antigens
   that didn't survive this treatement at all. So if you are going to
   test Dual Enzyme Blocking Reagent, keep this in mind and be sure to
   have the right controls involved.

   Chris van der Loos, PhD

   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389
   e-mail: [1]c.m.vanderloos <@t> amc.uva.nl


   ----- Original Message -----
   From  "Veronique Andriessen" <vandries <@t> vub.ac.be>
   Date  Fri, 28 Jan 2005 11:50:40 +0100
   To  "Histonet" <histonet <@t> lists.utsouthwestern.edu>, "Patsy Ruegg"
   <pruegg <@t> ihctech.net>
   Subject  RE: [Histonet] endogenous peroxidase blues

   Hi Patsy,
   I  have  encountered  somewhat  of  the  same  problem with endogenous
   peroxidase
   in  acetone  fixed  frozen  sections  of  acute pancreatitis, which is
   invaded
   with  white  blood cells. I tested several ways of blocking, home made
   and
   commercial including  glucose oxidase. I never got it to work properly
   (believe  me,  I've  tried!).  What worked for me in the end was a new
   product
   by  DAKO  called  "Dual  Endogenous  Enzyme  Blocking Reagent" (S2003)
   (Thank you
   Mr.  van der Loos!) It should work on cell preparations, frozen tissue
   and
   FFPE tissue (according to the manual). I only have tested it on frozen
   tissue.
   Just 10 minutes incubation at RT.
   This is just my honest opinion, I hope this can help you.
   Good luck
   Veronique Andriessen BAS
   Lab. Molecular Liver Cell Biology
   Free University Brussels (VUB), Belgium
   -----Original Message-----
   From: histonet-bounces <@t> lists.utsouthwestern.edu[

References

   1. mailto:c.m.vanderloos <@t> amc.uva.nl