[Histonet] Re: endogenous peroxidase blocking in acetone fixed preps.

[Brian Chelack]

I have had success using the following treatment:

1% H2O2 
0.1% Na Azide
in PBS 

add to acetone fixed section/cell prep/smear for 10-12
minutes

rinse 3X with PBS 

Carry on with blocking, primary antibody etc.


regards,
Brian Chelack