[Histonet] B-gal staining of frozen mouse tissue

[Jessica.Vaughn <@t> UCHSC.edu]

I am at wit's end when it comes to staining my frozen tissue of mouse lung (late stage embryo and newborn) for B-gal (w/ the enzyme, not Ab).  Actually, I know that the staining works, the problem I am having is that whenever I go through the staining procedure, my tissue falls off of the slides.  I have tried slides coated with gelatin, poly-l-lysine, and silane, and it never fails - the tissue falls off.  I don't know if it is some sort of chemical reaction that is taking place with the staining solution or what it happening.  I was just wondering if anyone else has had this problem and if anyone has any tips or tricks on how to overcome this issue.  Please let me know if you do or if you have any protocols I could try that work for you.

Thanks so much - I hope someone has some good advice for me before I lose my sanity!



Jessica J. Vaughn
Professional Research Assistant
Dept. of Pediatrics
University of Colorado Health Sciences Center