[Histonet] endogenous peroxidase blues

[Gayle Callis]

Glucose oxidase endogenous peroxidase and pseudoperoxidase block, I will 
send to you privately.  It works on cells, frozen sections AND paraffin 
sections.

At 03:09 PM 1/27/2005, you wrote:
>Is there a sure fire way of iliminating endogenous peroxidase from
>granulecytes in acetone fixed cytospins for hrp IHC?  Can I solve this
>problem without changing to a non-hrp detection enzyme?  I am trying to
>stain T lymphocytes in cytospins of sputum.  I do get cd3 positive
>lymphocyte looking cells, but agressive efforts has not completely blocked
>endogenous peroxidase in some of the larger cells with granules.
>Thanks for your help,
>Patsy
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)