[Histonet] Fluorescence staining

[Eva C Anderson]

Hi,
We are looking into trying some Fluorescence staining. We want to try a 
TSA Fluorescence System from PerkinElmer. The IHC protocol does however 
leave me with some questions.
We always dehydrate our samples after staining. The protocol however 
doesn't mention this. It only says that the samples can be 
counterstained and mounted after incubation with the Fluorophore 
Tyramide. Should we dehydrate and is there a particular mounting media 
we should use to prolong the time that fluorescence can be detected? Any 
help would be greatly appreciated.
Thanks in advance for all your help,
Eva
Georgetown University
Research Assistant