[Histonet] bubbles

[Janice A Mahoney]

First be sure there are no bubbles in the glycerol/gelatin.  Then be sure that the sliide is still wet (with distilled water) when you  coverslip.
Hope this helps.
Jan
Omaha

>>> Danielle Jin <zhuxx003 <@t> umn.edu> 01/24/2005 4:05:21 PM >>>
Hello,

I am new to histonet. I hope someone out there can help me solve my
problem.   When I do muscle staining (eg. acid phosphatase), the last
step is use glycerol Gelatin to cover the coverslip.  Somehow it always
trap some bubbles in the tissue section.  How can I get rid of the
bubbles?  Thanks

Danielle Jin



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