[Histonet] calling all muscle folks... PHOS coverslipping

[Smith, Allen]

In their original paper, Takeuchi and Kuriaki pointed out that one could
preserve the stain by adding iodine to the alcohols, xylene, and mounting
resin.  Haruo Machida, Edwin Perkins, and I took the trouble to find the
ideal iodine concentrations: 1 mg/ml of alcohol, xylene, or mounting resin.
We found that the best resin for this purpose was Clay-Adams' "Histoclad".
Harleco's HSR was the only other satisfactory mounting resin. (Durable
mounts of the iodine stain for the phosphorylase reaction, Stain Technology
41: 346-347, 1966) I was able to take publishable photographs of
amylo-phosphorylase reactions preserved this way 25 years after the slides
were made.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
Hofecker
Sent: Friday, January 07, 2005 12:22 PM
To: Celebre Julia
Cc: histonet posts
Subject: RE: [Histonet] calling all muscle folks... PHOS coverslipping


Hi Julia,
after applying the iodine and air drying, we use 85%
Karo syrup to put a coverslip on the slide.  It stays
readable for about a weeek, but never gets solid and
of course, is not considered permanent.  We have a big
gooey mess of PHOS slides in a drawer that are
unreadable.
I may try some glycerine.
Thanks for responding,
Jennifer


--- Celebre Julia <celebrej <@t> HHSC.CA> wrote:

> Hi there...
> Just curious, when you do coverslip your
> Phosphorylases is this permanent? 
> I personally don't coverslip mine, I grade them for
> the pathologist. But one
> of my co-workers would use a few drops of iodine
> with glycerine and place a
> coverslip on top...
> 
> Julia Celebre MLT
> Anatomic Pathology
> Hamilton General Hospital
> 905-527-0271  ext46145
> email: celebrej <@t> hhsc.ca
> 
> 
> -----Original Message-----
> From: Jennifer Hofecker [mailto:jhofecker <@t> yahoo.com]
> Sent: Friday, January 07, 2005 11:50 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] calling all muscle folks... PHOS
> coverslipping
> 
> 
> Hello to all muscle pros.
> I was wondering what you are using to coverslip your
> amylo-phosphorylase (PHOS) slides?  Any alternatives
> to 85% Karo syrup? 
> Thanks in advance,
> Jennifer
> 
> 
> 		
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