[Histonet] Formalin vapor fixation

[John Kiernan]

Daniel's 2003 paper in J Histochem Cytochem has
excellent coloured photomicrographs that show
what can be achieved by vapour fixation of
cryosections. It's worth looking up the full
text as well as the abstract.
                              John Kiernan
                              London, Canada
------------------------------------------------      
Daniel Eberhard wrote:
> 
> Cynthia,
> perhaps - 
http://www.jhc.org/cgi/content/abstract/51/3/401 
> - might help.
> Best,
> Daniel
> 
> Favara, Cynthia (NIH/NIAID) wrote:
> 
> >Has anyone ever fixed frozen sections with formaldehyde vapor? I have looked
> >in Bancroft and Stevens and they mention it as a suitable fixation. I can
> >not find a protocol. My intention is to place a cotton ball soaked with
> >0.5ml of NBF in the bottom of a coplin jar, put slides in cover a leave @RT
> >for 15 minutes.
> >
> >Sound good?
> >
> >C
> >
> >Cynthia Favara
> >NIAID/NIH/RML/LPVD
> >903 South 4th Street
> >Hamilton, MT 59840
> >406-363-9317
> >
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> Dr. Daniel Eberhard          =V=
> 
> Developmental Biology
> & Molecular Pathology
> 
> Graduate Programe on Pattern Formation
> 
> University of Bielefeld
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