[Histonet] PLP fixative

[RCHIOVETTI <@t> aol.com]

In a message dated 1/19/2005 5:12:42 PM US Mountain Standard Time, 
cindipqr <@t> wowway.com writes:

> does anyone have the recipe for PLP fixative and how it is used?
> After fixation can the tissue (brain) be processed thru paraffin as usual?
> Cindi Roberts
> Neurology Research
> Henry Ford Hospital
> 
> 


Cindi,

The recipe for PLP (Periodate-Lysine-Paraformaldehyde) fixative and a sample 
protocol (at least for immuno) using perfusion fixation is on the Chemicon 
website at:
http://www.chemicon.com/techsupp/Protocol/perfusion.asp

There are a few instances of using PLP with paraffin embedding, so the 
processing should work OK.  Whether you can retrieve reactivity from paraffin 
sections seems to be related to the antigen.  For example, see the NIEHS website:
http://dir.niehs.nih.gov/dirlep/immuno/protocols.htm

Cheers,

Bob Chiovetti