[Histonet] Myelin basic Protein in mouse

[Marceau, Gabriel (IAF)(LAVAL)]

Ray,

Serotec has a Rat anti-MBP under the catalogue number MCA408S but it is a culture supernatant, is this what you are using at the dilution of 1:2000? Usually supernatant are used pure or nearly-pure this is why I ask. If not can you still order number MCA408 even if no longuer in the catalogue?

Thanks,

Gabriel

------------------------------------------------------------
Gabriel Marceau
INRS-Institut Armand-Frappier
531, boul. des Prairies
Laval (Québec) H7V 1B7
Tél.: (450) 687-5010 poste 4282


From:	"Koelling, Ray"

Cynthia,
for a couple of years we've run rat-antiMBP from Serotec (MCA408) on mouse
brain fixed 24 hours in NBF.  At 1:1000-1:2000 overnight at 4 degrees with
no retrieval what-so-ever. Then biotinylated rabbit anti rat and so forth.
Ray
Raymond Koelling
Research Scientist, Pathology
Amgen Corp.
Seattle, WA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Favara,
Cynthia (NIH/NIAID)
Sent: Friday, January 07, 2005 11:21 AM
To: Histonet
Subject: [Histonet] Myelin basic Protein in mouse


I have run a quick search in the archives and found nothing. Anyone with
experience with Myelin Basic Protein  immunohistochemistry in FFPE murine
tissue?

Thanks,
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

Disclaimer: 
The information in this e-mail and any of its attachments is confidential
and may contain sensitive information. It should not be used by anyone who
is not the original intended recipient. If you have received this e-mail in
error please inform the sender and delete it from your mailbox or any other
storage devices. National Institute of Allergy and Infectious Diseases shall
not accept liability for any statements made that are sender's own and not
expressly made on behalf of the NIAID by one of its representatives