FW: [Histonet] endogenous alk phos

[Brett, Lawrence]

20%  Acetic Acid for 10 minutes does a good job on paraffins, but I've not
tried it on frozens. Might be too harsh. It also has the advantage that it
blocks ALL alk phos' including intestinal.

Lawrence Brett
Royal Infirmary, 
Edinburgh UK


-----Original Message-----
From: Emerson, Rachael [mailto:Rachael_Emerson <@t> URMC.Rochester.edu]
Sent: 18 January 2005 15:38
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] endogenous alk phos


Hello. Is there another method(s) to block endogenous alkaline phosphotase
in mice, on frozen sections, besides levamisole?

Thanks!!!!!

Rachael L. Emerson
Center for Human Genetics and Molecular Pediatric Diseases
University of Rochester Medical Center
575 Elmwood Avenue MRBX 1.11301
Rochester, NY 14642

Tel (585) 275-5073
Fax (585) 276-0232


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