[Histonet] Xylene Substitutes

[hymclab]

We have used Histosolve from Thermo Electron (formerly Thermo Shandon) for
over 10 years for all of our processing and staining.  We have had no
troubles with any applications including our immunos.
The only thing we use real xylene for anymore is the purge cycles on our
processors.

Dawn

-----Original Message-----
From: Randolph-Habecker, Julie [mailto:jhabecke <@t> seattlecca.org] 
Sent: Thursday, January 13, 2005 3:45 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Xylene Substitutes

Histonetters,

My lab is considering switching to a xylene substitute for tissue processing
and other routine use. Someone had mentioned that they tried to switch to a
xylene substitute a number of years ago and they had trouble getting a few
of their antibodies to work. Has anyone else had a similar problem? If so,
what substitute did you try and what antibodies were a problem?

Thanks,
Julie

Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org <mailto:jhabecke <@t> fhcrc.org>



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