[Histonet] endothelial cell marker other than PECAM

[Patsy Ruegg]

I use DAKO F8 on rat ffpe tissue using proteinase k digestion for 5 min.
with labelled polymer detection.
Patsy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Margaret
Horne
Sent: Thursday, January 13, 2005 3:10 AM
To: wen eng
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] endothelial cell marker other than PECAM

Hi , 
   I haven't started yet , but am about to try von Willebrand's factor ( 
Factor VIII  ).   I'll be doing it on rat,  first in FFPE to see if it
works, 
and then on osmium fixed Epon embedded.   Wish me luck!

              I chose Factor 8 because of a paper by Ulger et al , Anat 
Histol Embryol. 2002 Feb:31(1):31-5   where he compares various 
rat endothelial cell markers : Factor 8 , RECA-1, PECAM-1 , ICAM-
1, OX-43 and ZO-1.   

I am buying my anti body from  Biocare Medical and their tech support
suggests trying a pepsin treatment as they use it on the FFPE tissue at 37 C
for 5 min.  

               Hope this helps , don't hesitate to email me if you have any
more questions, 


       Margaret
Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I.,  C1A 4P3 
Canada


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet