[Histonet] stain for tight junctions

[Walters, Katherine S]

Hi all,

I have kind of an interesting problem.  We want to look at tight
junctions in cells.  We need a small molecule stain that will not be
taken up by the cells.  We want the stain to get between the cells and
be stopped by the tight junctions.  We do not want to use EM and we do
not want to use fluorescence.  Has anybody done this, or have an idea
about how to do this?

Thank you,
Kathy Walters
Central Microscopy Research Facility
University of Iowa